Roseobacter sinensis sp. nov., a marine bacterium capable to synthesize arachidonic acid

IF 1.8 3区生物学 Q4 MICROBIOLOGY

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology Pub Date : 2024-10-29 DOI:10.1007/s10482-024-02034-z

Hong-Chuan Wang, Meng-Han Huang, Dan-Yuan Guo, Wen-Li Liu, Zi-Wen Yang, Dao-Feng Zhang, Wen-Jun Li

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Abstract

Strain WL0113^T was isolated from surface seawater of the coast of Lianyungang, Jiangsu province, PR China. Strain WL0113^T shared highest 16S rRNA gene sequence similarity with Roseobacter insulae YSTF-M11^T (98.8%), followed by R. cerasinus AI77^T (98.8%), R. ponti MM-7^T (98.0%). Strain WL0113^T was Gram-stain-negative, cream, aerobic, non-motile and coccoid- to oval-shaped, and able to grow at pH 6.5–9.0 (optimum, pH 7.0–8.0), at 10–37 °C (optimum, 28 °C) and in the presence of 1–5% (w/v; optimum, 2.5%) NaCl. Ubiquinone-10 was detected as dominant. The main fatty acids (> 5%) of the strain WL0113^T were C_16:0, iso-C_17:0 3OH, C_20:4ω6,9,12,15c (arachidonic acid), and summed feature 8 (C_18:1ω7c and/or C_18:1ω6c). The major polar lipids include phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, glycophospholipid, unknown aminolipid, unknown phospholipid, and two unknown polar lipids. The ANI and dDDH values between strain WL0113^T and Roseobacter cerasinus were 80.4% and 23.0%, respectively. The genomic DNA G + C content of strain WL0113^T was 63.1%. Based on these data, it is proposed that strain WL0113^T represent novel species of the genus Roseobacter, for which the name Roseobacter sinensis sp. nov. is proposed. The type strain is WL0113^T (= GDMCC 1.3082^T = JCM 35567^T).

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一种能够合成花生四烯酸的海洋细菌--Roseobacter sinensis sp.

菌株 WL0113T 分离自中国江苏省连云港沿海的表层海水。菌株 WL0113T 与胰岛玫瑰杆菌 YSTF-M11T 的 16S rRNA 基因序列相似度最高（98.8%），其次是 R. cerasinus AI77T（98.8%）和 R. ponti MM-7 T（98.0%）。WL0113T 菌株为革兰氏阴性，乳白色，需氧，无运动性，茧状至椭圆形，能在 pH 6.5-9.0（最适 pH 7.0-8.0）、10-37 °C（最适 28 °C）和 1-5%（w/v；最适 2.5%）NaCl 的条件下生长。检测到的主要成分是泛醌-10。菌株 WL0113T 的主要脂肪酸（> 5%）为 C16:0、异 C17:0 3OH、C20:4ω6,9,12,15c（花生四烯酸）和总特征 8（C18:1ω7c 和/或 C18:1ω6c）。主要极性脂类包括磷脂酰甘油、二磷脂酰甘油、磷脂酰胆碱、糖磷脂、未知氨基脂、未知磷脂和两种未知极性脂类。WL0113T 菌株与 cerasinus 玫瑰杆菌的 ANI 值和 dDDH 值分别为 80.4% 和 23.0%。菌株 WL0113T 的基因组 DNA G + C 含量为 63.1%。基于这些数据，本文认为 WL0113T 菌株代表了玫瑰细菌属的新种，并将其命名为 Roseobacter sinensis sp.模式菌株为 WL0113T（= GDMCC 1.3082T = JCM 35567T）。

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来源期刊

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology 生物-微生物学

CiteScore

5.60

自引率

11.50%

发文量

104

审稿时长

3 months

期刊介绍： Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.