Enterovibrio qingdaonensis sp. nov. and Enterovibrio gelatinilyticus sp. nov., two marine bacteria isolated from surface seawater of Qingdao offshore

Two Gram-stain-negative, facultatively anaerobic, motile, and rod-shaped marine bacteria, designated strains ZSDZ35^T and ZSDZ42^T, were isolated from surface seawater of Qingdao offshore. Phylogenetic analysis of the 16S rRNA genes and whole genome data placed ZSDZ35^T and ZSDZ42^T within the genus Enterovibrio. Strain ZSDZ35^T was most closely related to Enterovibrio nigricans DSM 22720^T with 97.55% sequence similarity, whereas ZSDZ42^T was most closely related to Enterovibrio calviensis DSM 14347^T with 98.97% sequence similarity. Strain ZSDZ35^T grew with 0–8% (w/v) NaCl (optimum 4%), at 16–37 °C (optimum 28 °C) and pH 6.0–9.0 (optimum pH 8.0); whereas strain ZSDZ42^T grew with 1–7% (w/v) NaCl (5%), at 4–28 °C (8 °C) and pH 6.0–9.0 (pH 7.0). Both strains shared the same major fatty acid components of summed feature 3 (C_16:1ω7c or/and C_16:1ω6c), summed feature 8 (C_18:1ω7c and C_18:1ω6c) and C_16:0, with different proportions. The DNA G + C contents of strains ZSDZ35^T and ZSDZ42^T were 47.2% and 46.7%, respectively. Based on the results of polyphasic analyses, ZSDZ35^T and ZSDZ42^T are considered to represent novel species, for which the names Enterovibrio qingdaonensis sp. nov. (type strain, ZSDZ35^T = MCCC 1K06293^T = KCTC 82887^T) and Enterovibrio gelatinilyticus sp. nov. (type strain, ZSDZ42^T = MCCC 1K06294^T = KCTC 82886^T) are proposed, respectively.