Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology最新文献

{"title":"Molecular characterization of lumpy skin disease virus in the cattle population of District Lower Chitral, Khyber Pakhtunkhwa, Pakistan","authors":"Almas Faryal Nizam,&nbsp;Iram Maqsood,&nbsp;Hanif Ur Rahman,&nbsp;Saira Awaz,&nbsp;Imran Ullah Shah,&nbsp;Muhammad Ijaz Ali,&nbsp;Baitullah Khan,&nbsp;Gul Muhammad,&nbsp;Asima Azam,&nbsp;Ayesha Hidayat","doi":"10.1007/s10482-025-02137-1","DOIUrl":"10.1007/s10482-025-02137-1","url":null,"abstract":"<div><p>Lumpy skin disease (LSD) is a transboundary viral infection of cattle caused by the lumpy skin disease virus (LSDV), a member of the <i>Poxviridae</i> family in genus <i>Capripox</i>. In Pakistan, after its first outbreak in 2021, the disease caused significant financial damage to the livestock sector. The current study was designed to investigate LSDV and its histopathological lesions in the LSDV infected cattle population of the Lower Chitral district of Khyber Pakhtunkhwa, Pakistan. Overall, 384 samples were collected, including nasal swabs, blood, and skin nodules from LSDV suspected cattle. These samples were subjected to Polymerase Chain Reaction (PCR) by targeting the p32 and ORF036 coding regions in the virus. Out of 384 samples, 71 were positive for both coding regions. The phylogenetic analysis revealed that virus strains from neighboring countries shared a common cluster with PQ067260 from Chitral, indicating the close genetic relationship between them. Seasonal analysis showed the highest prevalence of LSDV in summer (23%). Additionally, our research identified that the virus is more likely to infect females (32%) than males, animals younger than one year (25%) than older than one year, and cross cattle breeds (22%) than other breeds. Our results also found variations in disease prevalence across different regions of Chitral. Subsequent SPSS analysis demonstrated that gender (&lt; 0.0001), age (0.0002), season (0.0004), and breed (&lt; 0.0001) had a significant impact on the prevalence of the disease. Histopathology indicated presence of eosinophils, vacuolar degeneration, necrosis, and leukocytic infiltration, in diseased tissues. Further research needs to be conducted on the isolation of the field virus and local vaccine development to control this highly contagious and economically important viral disease in the country.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144776812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of plant growth-promoting bacteria (PGPB) in enhancing phenolic compounds biosynthesis and its relevance to abiotic stress tolerance in plants: a review","authors":"Zuzanna Jakubowska,&nbsp;Marcin Gradowski,&nbsp;Jakub Dobrzyński","doi":"10.1007/s10482-025-02130-8","DOIUrl":"10.1007/s10482-025-02130-8","url":null,"abstract":"<div><p>Biofortification of plants using Plant Growth-Promoting Bacteria (PGPB) represents a promising strategy in sustainable agriculture. This paper discusses the PGPB action in the context of their impact on phenolic compounds biosynthesis and the prospects for their application in agriculture. So far, no review article has summarized the significance of PGPB in increasing phenolic compounds in plants. PGPB, such as <i>Pseudomonas</i>, <i>Bacillus</i>, and <i>Azospirillum</i>, promote plant growth by producing phytohormones, enhancing nutrient availability, and stimulating the biosynthesis of secondary metabolites through the activation of Induced Systemic Resistance (ISR). The activation of ISR (Induced Systemic Resistance) by PGPB stimulates the phenylpropanoid pathway, which is the primary biosynthetic route for polyphenolic compounds, including phenolic acids and flavonoids, in plants. Studies indicate that PGPB may increase phenolic compounds content from 9% to over 200%, while simultaneously improving antioxidant activity. Through the secretion of phenolic compounds, PGPB also can mitigate abiotic stresses such as drought, salinity and heavy metal contamination. Among the phenolic compounds whose production in various plant parts can be stimulated by PGPB are flavonoids, such as quercetin, procyanidin B1, EGCG, and catechin, and phenolic acids, including caffeic acid, ferulic acid, and chlorogenic acid. Advancements in omics research will enable a more precise investigation of the impact of PGPB, including endophytic bacteria, on the biosynthetic pathways of phenolic compounds. In the future, this will translate into improved efficiency in stimulating the production of these compounds. Nevertheless, even now, the use of PGPB offers a sustainable alternative to genetic engineering, reducing reliance on chemical inputs in agriculture.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12289753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144709756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and taxonomic characterization of Niallia pakistanensis sp. nov. NCCP-28T: a novel antibiotic-resistant and heavy-metal-tolerant bacterium isolated from the legume rhizosphere in Pakistan","authors":"Sadia Manzoor,&nbsp;Saira Abbas,&nbsp;Sobia Zulfiqar,&nbsp;Dildar Wu,&nbsp;Hong-Chuan Wang,&nbsp;Wen-Jun Li,&nbsp;Muhammad Arshad,&nbsp;Iftikhar Ahmed","doi":"10.1007/s10482-025-02132-6","DOIUrl":"10.1007/s10482-025-02132-6","url":null,"abstract":"<div><p>Soil microbes play a crucial role in biogeochemical cycling and are a valuable resource for discovering novel taxa with environmental applications. This study aimed to explore rhizospheric microbial diversity for potential biotechnological and ecological relevance. The taxonomic placement of strain NCCP-28^T was determined through polyphasic characterization. Isolated from the rhizospheric soil of a legume, strain NCCP-28^T exhibited Gram-positive, motile, aerobic, and rod-shaped cells that produced endospores. Additionally, it tested negative for oxidase but positive for catalase. The cells were grown at a pH range of 6‒10 (optimum of 7.0) or over a 20‒45 °C range (optimum of 30 °C) and could thrive in 0‒10% NaCl (optimum of 1% w/v). Strain NCCP-28^T also tolerated heavy metals, including chromium (450 ppm), copper (300 ppm), and zinc (550 ppm). Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain NCCP-28^T is closely related to Niallia nealsonii DSM 15077^T, exhibiting 97.79% sequence similarity. However, strain NCCP-28^T formed a distinct and well-supported clade in the phylogenomic tree, clearly separating it from its nearest relatives. This distinction was further substantiated by whole-genome comparisons, including digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values, which collectively support the classification of strain NCCP-28^T as a novel species within the genus Niallia. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unknown amino phospholipids were included in the polar lipid profile of the strain. The predominant fatty acids (&gt; 10%) included anteiso-C_15:0, iso-C_15:0, and C_16:0. However, MK-7 and MK-8 are respiratory quinones. Strain NCCP-28^T classified as a distinct species within the genus <i>Niallia</i> based on its phylogenetic, phenotypic, chemotaxonomic and genotypic traits and was designated the type strain NCCP-28^T (CGMCC1.62018^T = GDMCC1.4390^T), which was named <i>Niallia pakistanensis</i> sp. nov. These findings emphasize microbial systematics, environmental microbiology, and functional genomics. The ecological origin, genomic insights, and adaptive traits of strain NCCP-28^T support its potential application in environmental sustainability and microbial biotechnology.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144700263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of pure mycelium materials from different mushroom-forming fungi","authors":"Jeroen G. van den Brandhof,&nbsp;Noortje Hansen,&nbsp;Chen Hou,&nbsp;Sander C. Broers,&nbsp;Martin Tegelaar,&nbsp;Han A. B. Wösten","doi":"10.1007/s10482-025-02133-5","DOIUrl":"10.1007/s10482-025-02133-5","url":null,"abstract":"<div><p>Pure mycelium materials (PMMs) are a sustainable alternative for a range of non-sustainable products such as textile, leather and meat. So far, properties of PMMs of different fungi have not been systematically assessed. Here, 11 mushroom-forming fungi, of which 10 isolated from nature, were grown in liquid shaken cultures. <i>Schizophyllum commune, Ganoderma resinaceum</i>, and <i>Trametes betulina</i> produced the highest biomass (8.62, 7.58, and 6.94 g L⁻¹, respectively) when grown as mono-cultures in malt extract broth. Therefore, PMM properties were determined of mono-cultures, mixed-cultures, and co-cultures of these three fungi. The maximum tensile strength of the PMMs of the mono-cultures, mixed-cultures, and co-cultures of <i>S. commune</i>, <i>G. resinaceum</i>, and <i>T. betulina</i> did not show significant differences and ranged between 4.5 to 6.3 MPa. The elongation at break of the different PMMs was generally low and ranged between 0.8 and 1.6%. The Young’s modulus of the PMMs also showed relatively small differences ranging between 408 and 710 MPa. The <i>G. resinaceum</i> PMMs showed the lowest water uptake, while the <i>S. commune</i> mono-, mixed- and co-culture PMMs showed the highest water contact angle. Together, it is concluded that the properties of the mono-, mixed-, and co-cultures of <i>S. commune</i>, <i>G. resinaceum</i>, and <i>T. betulina</i> are not very different. These data suggest that the species of mushroom forming fungi does not have a major impact on PMM properties of biomass from liquid shaken cultures.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12287172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144692325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizosphere microbiota adaptation under blueberry cultivation: modified paddy soil versus acidic red soil","authors":"Guoao Ding,&nbsp;Wenqian Weng,&nbsp;Xianglong Feng,&nbsp;Yue Gao,&nbsp;Yan Zhang","doi":"10.1007/s10482-025-02128-2","DOIUrl":"10.1007/s10482-025-02128-2","url":null,"abstract":"<div><p>Although rhizosphere microbiota play pivotal roles in the adaptation of blueberry to acidic soils, their adaptation to contrasting soil types remains unexplored. Herein, rhizosphere soils from modified paddy and red soil plantations were analysed via 16S sequencing, core microbiome identification and KEGG pathway mapping. These analyses revealed that modified paddy soil exhibited higher α-diversity (Shannon index: <i>p</i> = 0.037) than red soil. The analysis of β-diversity confirmed significant divergence between the red soil rhizosphere communities and their bulk soil counterparts (<i>p</i> &lt; 0.05), whereas the modified paddy soil communities remained structurally stable. Core OTU analysis identified 96 shared taxa (18.2% of total OTUs) across soils. The red soil rhizosphere microbes prioritised energy-yielding pathways (e.g. glucose-1-phosphate and sucrose degradation) that are critical for fruit cell wall synthesis. The modified paddy soil communities favoured glycogen degradation, reflecting the existence of resource competition under acidic stress. This study aimed to characterize the rhizosphere microbiota of blueberries cultivated in two distinct acidic soil types (modified paddy soil and red soil), identify core microbiome members, and elucidate their functional adaptations, thereby emphasising the importance of tailored microbial management during soil modification strategies (e.g. paddy soil acidification) to meet plant growth requirements.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144692327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paenibacillus peoriae: current knowledge and agricultural biotechnology potential of a close relative of P. polymyxa","authors":"Jakub Dobrzyński,&nbsp;Iryna Kulkova","doi":"10.1007/s10482-025-02135-3","DOIUrl":"10.1007/s10482-025-02135-3","url":null,"abstract":"<div><p><i>Paenibacillus peoriae</i>, a member of the genus <i>Paenibacillus</i>, is a gram-positive, spore-forming bacterium closely related to <i>Paenibacillus polymyxa</i>. This species exhibits a wide range of metabolic capabilities, enabling it to thrive in diverse environments and produce bioactive compounds with potential applications in agriculture and biotechnology. Recent studies reveal its capacity to produce biocontrol agents, such as fusaricidins, polymyxins, and tridecaptins, along with hydrolytic enzymes that inhibit phytopathogens including <i>Fusarium</i>, <i>Rhizoctonia</i>, <i>Alternaria</i>, <i>Botrytis</i>, <i>Phytophthora</i> (<i>Oomycota</i> phylum). Additionally, this species was capable of directly promoting plant growth through various mechanisms, such as the production of indole-3-acetic acid (IAA), nitrogen fixation, phosphorus solubilization, and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity. <i>P. peoriae</i> strains also produce enzymes like cellulase and protease, essential for various industrial applications, and extracellular polysaccharides (EPS) demonstrating potential in bioremediation and heavy metal removal. Recent studies highlight its ability to synthesize 2,3-butanediol, a valuable industrial compound, further establishing its biotechnological significance. This review consolidates current knowledge on the genome, metabolites, and applications of <i>P. peoriae</i> while identifying research gaps and future directions for maximizing its potential in sustainable agriculture and biotechnology.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12287138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144692326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial and anticancer activities of rhizobacterial lipopeptides against carcinogenic bacteria","authors":"Nawal E. Al-Hazmi","doi":"10.1007/s10482-025-02131-7","DOIUrl":"10.1007/s10482-025-02131-7","url":null,"abstract":"<div><p>Rhizosphere-derived lipopeptides, including surfactin, iturin, and fengycin from <i>Bacillus</i> spp., exhibit potent antibacterial and anticancer properties. This study evaluated the antibacterial activity, mechanism of action, anti-biofilm effects, and anticancer potential of these lipopeptides individually and in combination. The minimum inhibitory concentration (MIC) against pathogenic Gram-negative bacteria (<i>Bacteroides fragilis</i>, <i>Escherichia coli</i>, <i>Fusobacterium nucleatum</i>, <i>Helicobacter pylori</i>, <i>Neisseria gonorrhoeae</i>, <i>Porphyromonas gingivalis</i>, and <i>Salmonella enterica</i>) was determined using the broth dilution method. Among the single lipopeptides, fengycin demonstrated the strongest antibacterial effect, whereas the combination of all three lipopeptides exhibited the lowest MIC values, reducing bacterial growth by approximately 95% more than chloramphenicol. Mechanistically, lipopeptide treatments increased bacterial membrane permeability, with the three-lipopeptide mixture inducing 60% greater electrolyte leakage than chloramphenicol. Additionally, lipopeptides significantly reduced bacterial DNA content, with the three-lipopeptide combination leading to a 321% greater reduction than chloramphenicol. Biofilm formation was also markedly inhibited, particularly by fengycin and the lipopeptide mixtures. Furthermore, the anticancer activity of lipopeptides was assessed using the MTT assay against gastric, pancreatic, liver, colon, esophageal, and breast cancer cell lines. The three-lipopeptide mixture exhibited the highest cytotoxicity, with IC50 values approximately 82% lower than those of doxorubicin. These findings highlight the potential of rhizosphere lipopeptides as promising antibacterial and anticancer agents, warranting further investigation for therapeutic applications.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zhengella sedimenti sp. nov. and Phycobacter sedimenti sp. nov., two novel bacteria isolated from coastal sediment with genomic and metabolic analysis","authors":"Pei-Ran Lin,&nbsp;Li-Jia-Yu Deng,&nbsp;Han-Zhe Zhang,&nbsp;Le Liu,&nbsp;Tian-He Liu,&nbsp;De-Chen Lu,&nbsp;Zong-Jun Du","doi":"10.1007/s10482-025-02120-w","DOIUrl":"10.1007/s10482-025-02120-w","url":null,"abstract":"<div><p>In this study, two novel Gram-stain-negative bacterial strains, K97^T and ZM62^T, were isolated from sediment samples collected along the coast of Weihai, China, and described using polyphasic taxonomic techniques. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain K97^T exhibited the highest sequence similarity (98.34%) with <i>Phycobacter azelaicus</i> F10^T within the genus <i>Phycobacter</i>, followed by <i>Pseudooceanicola marinus</i> AZO-C^T (97.14%) and <i>Phaeobacter italicus</i> LMG24365^T (96.85%). Strain ZM62^T exhibited the highest sequence similarity (98.53%) with <i>Zhengella mangrovi</i> X9-2-2^T within the genus <i>Zhengella</i>, followed by <i>Phyllobacterium myrsinacearum</i> NBRC 100019^T (96.49%) and <i>Oricola thermophila</i> MEBiC13590^T (96.35%). The respiratory quinone was Q-10 for both strains. The major fatty acid in both strains K97^T and ZM62^T is Summed Feature 8 (C_18:1<i>ω</i>6c/C_18:1<i>ω</i>7c). The main polar lipids for strain K97^T included diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), and phosphatidylglycerol (PG), while for strain ZM62^T, the main polar lipids included diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), and phosphatidylcholine (PC). Based on the polyphasic taxonomic data, strain K97^T is proposed as a novel species within the genus <i>Phycobacter</i>, for which the name <i>Phycobacter sedimenti</i> is proposed, and the type strain is K97^T (= KCTC 8365^T = MCCC 1H01460^T). Strain ZM62^T is proposed as a novel species within the genus <i>Zhengella</i>, for which the name <i>Zhengella sedimenti</i> is proposed, and the type strain is ZM62^T (= KCTC 8813^T = MCCC 1H01495^T). Additionally, genomic and metabolic analyses revealed that the genus <i>Phycobacter</i> possesses DMSP synthesis and metabolism genes and a complete CMP-KDO pathway, indicating potential symbiosis with algae. Metabolic analysis of strain ZM62^T indicates its potential role in the degradation of xenobiotic compounds, supported by the presence of annotated pathways for aminobenzoate (ko00627) and toluene (ko00623) degradation.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144668906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The DR2416/DR2415 two-component system is responsible for the radioresistance of Deinococcus radiodurans","authors":"Wuzhou Wang,&nbsp;Zhenfang Du,&nbsp;Fei Yang,&nbsp;Shuya He","doi":"10.1007/s10482-025-02125-5","DOIUrl":"10.1007/s10482-025-02125-5","url":null,"abstract":"<div><p>Two-component system (TCS) plays a crucial role in translating stimulus signals into specific adaptive responses and aids prokaryotic organisms in withstanding diverse stresses. <i>Deinococcus radiodurans</i> is renowned for its exceptional radioresistance and has become a valuable model for studying DNA repair, bioremediation and planetary protection space missions. TCSs are well-developed by this bacterium, thus the role in its radioresistance can be envisaged. DR2416 was predicted to be a histidine kinase, and DR2415, a response regulator. Our study revealed that deleting <i>dr2416</i> or (and) <i>dr2415</i> reduced <i>D. radiodurans</i>' resistance to various DNA damage agents, including ionizing radiation, hydrogen peroxide, and mitomycin C. The DR2416 protein was situated on the membrane of <i>D. radiodurans</i>. Western blotting analysis revealed that DR2415 was significantly induced by ionizing radiation. The Phos-tag SDS-PAGE assay illustrated that DR2416 could undergo self-phosphorylation at His259 in vitro and subsequently transfer the phosphoryl group to the Asp52 residue of DR2415. The qRT-PCR method showed that the DR2416/DR2415 TCS regulated genes related to DNA double-strand break repair and reactive oxygen species (ROS) removal, including <i>recN</i> (<i>dr1477</i>), <i>sbcC</i> (<i>dr1922</i>), and <i>katE</i> (<i>dra0259</i>). In conclusion, our findings suggested that the DR2416/DR2415 TCS might provide radioresistance to <i>D. radiodurans</i> through DNA damage repair and ROS scavenging mechanisms.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ewingella allii sp. nov. isolated from a diseased onion plant in the Columbia Basin of Washington State, USA","authors":"Fanele Cabangile Mnguni,&nbsp;Gi Yoon Shin,&nbsp;Lindsey J. du Toit,&nbsp;Michael L. Derie,&nbsp;Teresa A. Coutinho","doi":"10.1007/s10482-025-02116-6","DOIUrl":"10.1007/s10482-025-02116-6","url":null,"abstract":"<div><p>Isolation of strain 20WA0182^T from a diseased onion plant grown in the Columbia Basin of Washington State, USA, led to preliminary identification as a member of the genus <i>Ewingella</i>. The strain was characterised as a Gram-stain-negative, facultative anaerobe that is rod-shaped, motile with polar flagella, catalase positive, and oxidase negative. The strain 20WA0182^T isolated was pathogenic to yellow onion bulbs, weakly pathogenic on onion leaves of the cv. Ranchero, and caused a pathogenic response using the red onion bulb scale necrosis assay. Phylogenetic analyses using the 16S rRNA gene and four housekeeping genes, <i>atpD, gyrB, infB,</i> and <i>rpoB,</i> showed that strain 20WA0182^T formed a branch that clustered with <i>E. americana</i> strains, but on a separate node, indicating it is a novel species of this genus. Whole-genome sequencing of strain 20WA0182^T revealed a genome size of 4,604,541 nt, with 25 contigs and a G + C content of 53.8%, strain 20WA0182^T was 99.2% complete. The average nucleotide identity of strain 20WA0182^T compared with <i>E</i>. <i>americana</i> strains scores ranged from 92.85 to 93.96%, below the 95% threshold to classify strains as the same species. Similarly, dDDH scores were 56.0 to 56.2%, less than the 70% threshold required to delineate prokaryotes as the same species. Strain 20WA0182^T and <i>Ewingella</i> sp. CoE-038-23 shared the ANI score above 97.59% and 81.0% dDDH score to be classified as a novel species of <i>Ewingella</i>. As the type strain 20WA0182^T (= BD 3290^T = LMG 33618^T) was pathogenic to onion bulbs and leaves, the name <i>Ewingella allii</i> is proposed. GenBank accession number = JAWUDN000000000.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12267313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144644076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}