Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology最新文献

{"title":"Two new species of Fusarium in the F. incarnatum-equiseti species complex from Oryza sativa in Iran","authors":"Saeid Afzalinia,&nbsp;Mehdi Mehrabi-Koushki,&nbsp;Reza Farokhinejad","doi":"10.1007/s10482-024-02019-y","DOIUrl":"10.1007/s10482-024-02019-y","url":null,"abstract":"<div><p>Identification of <i>Fusarium</i> species associated with diseases symptoms in plants is an important step toward understanding the ecology of plant–fungus associations. In this study, four <i>Fusarium</i> isolates were obtained from root rot of <i>Oryza sativa</i> L. in Izeh (southwest of Iran) and identified based on phylogenetic analyses combined with morphology. Phylogenetic analyses based on combined translation elongation factor 1-α, calmodulin, RNA polymerase II second largest subunit, and Beta-tubulin (<i>tub2</i>) sequence data delimited two new species, namely <i>F. khuzestanicum</i> and <i>F. oryzicola</i> spp. nov., from previously known species of <i>Fusarium</i> within <i>F. incarnatum</i>-<i>equiseti</i> species complex (FIESC). Morphologically, <i>F. khuzestanicum</i> produces the macroconidia with distinctly notched to foot-shaped basal cells, while basal cells in the macroconidia of <i>F. oryzicola</i> are more extended and distinctly elongated foot shape. Furthermore, these two new species are distinguished by the size of their sporodochial phialides and macroconidia. The results of the present show that the FIESC species complex represent more cryptic species.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142254698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yunchengibacter salinarum gen. nov., sp. nov., a novel bacterium of the family Kordiimonadaceae isolated from sediment in Yuncheng salt lake","authors":"Hao-Ran Guo,&nbsp;Fan Wang,&nbsp;Hui-Ying Yu,&nbsp;Chuan-Xu Wang,&nbsp;Zhuo Wang,&nbsp;Bao-Zhu Fang,&nbsp;Xin Li,&nbsp;Wen-Jun Li","doi":"10.1007/s10482-024-02011-6","DOIUrl":"10.1007/s10482-024-02011-6","url":null,"abstract":"<div><p>A Gram-stain-negative, aerobic, motile and rod-shaped bacterium, the color of the bacterial colony ranges from light yellow to yellow, designated YC-2023-2T, was isolated from sediment sample of Yuncheng salt lake. Growth occurred at 15–45℃ (optimum 37℃), pH 6.0-9.0 (optimum pH 7.0-8.0) and with 0–8.0% NaCl (w/v, optimum 2.0%). The phylogenetic analysis based on 16S rRNA gene sequences showed that strain YC-2023-2T belonged to the family Kordiimonadaceae. The closely related members were Gimibacter soli 6D33T (92.38%), Kordiimonas lipolytica M41T (91.88%), Eilatimonas milleporae DSM 25217T (91.88%) and Kordiimonas gwangyangensis JCM 12864T (91.84%). The genome of strain YC-2023-2T was 2957513 bp, and the genomic DNA G+C content was 63.91%. The main respiratory quinone was Q-10 and the major fatty acids (&gt;10%) were iso-C15:0, C16:0, C19:0 cyclo ω8c, Summed Feature 8 (C18:1 ω6c or C18:1 ω7c) and Summed Feature 9 (iso-C17:1 ω9c or C16:0 10-methyl). The major polar lipids consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, unidentified glycolipid, unidentified lipid, and two unidentified aminolipids. Based on the phylogenetic, phenotypic and chemotaxonomic characteristics, strain YC-2023-2T is proposed to represent a novel species of a novel genus named Yunchengibacter salinarum gen. nov., sp. nov., within the family Kordiimonadaceae. The type strain is YC-2023-2T (= GDMCC 1.4502T = KCTC 8546T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gallium: a decisive “Trojan Horse” against microorganisms","authors":"Amanda Stefanie Jabur de Assis,&nbsp;Guilherme Manassés Pegoraro,&nbsp;Iolanda Cristina Silveira Duarte,&nbsp;Tiago Palladino Delforno","doi":"10.1007/s10482-024-02015-2","DOIUrl":"10.1007/s10482-024-02015-2","url":null,"abstract":"<div><p>Controlling multidrug-resistant microorganisms (MRM) has a long history with the extensive and inappropriate use of antibiotics. At the cost of these drugs being scarce, new possibilities have to be explored to inhibit the growth of microorganisms. Thus, metallic compounds have shown to be promising as a viable alternative to contain pathogens resistant to conventional antimicrobials. Gallium (Ga³⁺) can be highlighted, which is an antimicrobial agent capable of disrupting the essential activities of microorganisms, such as metabolism, cellular respiration and DNA synthesis. It was observed that this occurs due to the similar properties between Ga³⁺ and iron (Fe³⁺), which is a fundamental ion for the correct functioning of bacterial activities. The mimetic effect performed by Ga³⁺ prevents iron transporters from distinguishing both ions and results in the substitution of Fe³⁺ for Ga³⁺ and in adverse metabolic disturbances in rapidly growing cells. This review focuses on analyzing the development of research involving Ga³⁺, elucidating the intracellular incorporation of the “Trojan Horse”, summarizing the mechanism of interaction between gallium and iron and comparing the most recent and broad-spectrum studies using gallium-based compounds with antimicrobial scope.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tapping into haloalkaliphilic bacteria for sustainable agriculture in treated wastewater: insights into genomic fitness and environmental adaptation","authors":"Yosra Amara,&nbsp;Mouna Mahjoubi,&nbsp;Yasmine Souissi,&nbsp;Hanene Cherif,&nbsp;Islem Naili,&nbsp;Darine ElHidri,&nbsp;Imen Kadidi,&nbsp;Amor Mosbah,&nbsp;Ahmed S Masmoudi,&nbsp;Ameur Cherif","doi":"10.1007/s10482-024-02012-5","DOIUrl":"10.1007/s10482-024-02012-5","url":null,"abstract":"<div><p>The increasing salinity and alkalinity of soils pose a global challenge, particularly in arid regions such as Tunisia, where about 50% of lands are sensitive to soil salinization. Anthropogenic activities, including the use of treated wastewater (TWW) for irrigation, exacerbate these issues. Haloalkaliphilic bacteria, adapted to TWW conditions and exhibiting plant-growth promotion (PGP) and biocontrol traits, could offer solutions. In this study, 24 haloalkaliphilic bacterial strains were isolated from rhizosphere sample of olive tree irrigated with TWW for more than 20 years. The bacterial identification using 16S rRNA gene sequencing showed that the haloalkaliphilic isolates, capable of thriving in high salinity and alkaline pH, were primarily affiliated to <i>Bacillota</i> (<i>Oceanobacillus</i> and <i>Staphylococcus</i>). Notably, these strains exhibited biofertilization and enzyme production under both normal and saline conditions. Traits such as phosphate solubilization, and the production of exopolysaccharide, siderophore, ammonia, and hydrogen cyanide were observed. The strains also demonstrated enzymatic activities, including protease, amylase, and esterase. Four selected haloalkaliphilic PGPR strains displayed antifungal activity against <i>Alternaria terricola</i>, with three showing tolerances to heavy metals and pesticides. The strain <i>Oceanobacillus picturea</i> M4W.A2 was selected for genome sequencing. Phylogenomic analyses indicated that the extreme environmental conditions probably influenced the development of specific adaptations in M4W.A2 strain, differentiating it from other <i>Oceanobacillus picturae</i> strains. The presence of the key genes associated with plant growth promotion, osmotic and oxidative stress tolerance, antibiotic and heavy metals resistance hinted the functional capabilities might help the strain M4W.A2 to thrive in TWW-irrigated soils. By demonstrating this connection, we aim to improve our understanding of genomic fitness to stressed environments. Moreover, the identification of gene duplication and horizontal gene transfer events through mobile genetic elements allow the comprehension of these adaptation dynamics. This study reveals that haloalkaliphilc bacteria from TWW-irrigated rhizosphere exhibit plant-growth promotion and biocontrol traits, with genomic adaptations enabling their survival in high salinity and alkaline conditions, offering potential solutions for soil salinization issues.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbacterium rhizophilus sp. nov., an indole acetic acid-producing actinobacterium isolated from rhizosphere soil","authors":"Haifei Liu,&nbsp;Quan Yang,&nbsp;Jiawei Li,&nbsp;Lifang Yang,&nbsp;Aolin Zhao,&nbsp;Ying Huang,&nbsp;Hongcun Liu,&nbsp;Shujing Wu,&nbsp;Mingguo Jiang","doi":"10.1007/s10482-024-02014-3","DOIUrl":"10.1007/s10482-024-02014-3","url":null,"abstract":"<div><p>A novel gram-stain-positive, short rod, aerobic, non-motile and non-spore-forming actinobacterial strain, designated GXG1230^T was isolated from the rhizosphere soil of a coastal mangrove forest in Beihai city, Guangxi Zhuang Autonomous Region, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GXG1230^T was affiliated with the genus <i>Microbacterium</i>. Additionally, it demonstrated a high degree of similarity to <i>Microbacterium paludicola</i> US15^T (97.9%) and <i>Microbacterium marinilacus</i> YM11-607^T (97.3%). Chemotaxonomic characteristics showed that the whole-cell sugars were glucose, xylose, rhamnose and galactose. Menaquinones MK-11 and MK-12 were detected as respiratory quinones. Lysine was found in the peptidoglycan hydrolysate and the polar lipids were diphosphatidylglycerol, one phospholipid and two unidentified glycolipid. The major fatty acids were <i>anteiso</i>-C_15:0, <i>iso</i>-C_16:0 and <i>anteiso</i>-C_17:0. The strain GXG1230^T exhibited a genomic DNA G + C content of 71.7%. Furthermore, the average nucleotide identity values of GXG1230^T with the reference strains were 75.4% and 81.9%, respectively, while the digital DNA-DNA hybridization values were 20.1% and 25.0%. Based on physiological, chemotaxonomic and phylogenetic information, strain GXG1230^T is considered to represent a novel species of the genus <i>Microbacterium</i>, for which the name <i>Microbacterium rhizophilus</i> sp.nov is proposed, with GXG1230^T (= MCCC 1K09302^T = KCTC 59252^T) as the type strain.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10482-024-02014-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial community diversity analysis of kiwifruit pollen and identification of potential pathogens","authors":"Jiaqi Yang,&nbsp;Manli Yao,&nbsp;Dan Zhang,&nbsp;Yu Zhao,&nbsp;Guitian Gao","doi":"10.1007/s10482-024-02013-4","DOIUrl":"10.1007/s10482-024-02013-4","url":null,"abstract":"<div><p>The kiwifruit industry typically uses commercial pollen for artificial pollination. However, during the collection of male flowers and pollen production, pollen can be easily contaminated by pathogenic bacteria that cause diseases such as canker and flower rot. Consequently, it is crucial to understand the structure of the pollen microbial community. This study employed Illumina high-throughput sequencing technology to analyze the fungal and bacterial composition in pollen samples from various regions in Shaanxi Province. Concurrently, potential pathogenic strains were isolated using traditional microbial isolation and cultivation techniques, and their molecular identification was performed through 16S rDNA sequence analysis. A tieback test was conducted on healthy branches to verify the pathogenicity of the strains. The results revealed a rich diversity of fungi and bacteria in kiwifruit pollen. At the phylum level, pollen fungi were mainly distributed in Ascomycota, and bacteria were mainly distributed in Proteobacteria and Firmicutes. The dominant fungal genera were <i>Mycosphaerella</i>, <i>Aspergillus</i>, and <i>Cladosporium;</i> the dominant bacterial genera were <i>Weissella</i>, <i>Pantoea</i>, <i>Enterobacter</i>, and <i>Pseudomonas</i>, respectively. Additionally, both <i>Erwinia persicina</i> and <i>Pseudomonas fluorescens</i>, isolated from pollen, exhibited high pathogenicity toward healthy kiwifruit branches. These findings contribute to a deeper understanding of the microbial diversity in commercial kiwifruit pollen used for mass pollination.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142009918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genus Coelastrella (Chlorophyceae, Chlorophyta): molecular species delimitation, biotechnological potential, and description of a new species Coelastrella affinis sp. nov., based on an integrative taxonomic approach","authors":"E. Krivina,&nbsp;M. Sinetova,&nbsp;E. Zadneprovskaya,&nbsp;M. Ivanova,&nbsp;A. Starikov,&nbsp;K. Shibzukhova,&nbsp;E. Lobakova,&nbsp;Yu. Bukin,&nbsp;A. Portnov,&nbsp;A. Temraleeva","doi":"10.1007/s10482-024-02008-1","DOIUrl":"10.1007/s10482-024-02008-1","url":null,"abstract":"<div><p>Despite the long research history on the genus <i>Coelastrella</i>, its species diversity and biotechnological potential have not been fully explored. For the first time, cluster analysis of morphological characteristics was done in the representatives of the said genus. The results obtained have shown that morphological similarity does not necessarily indicate a molecular genetic relationship. It the light of it, the taxonomic status of species can reliably be determined using specific DNA region, such as 18S–ITS1–5.8S–ITS2. The V4 and V9 regions of gene 18S rRNA are relatively conservative fragments which are not suitable for species identification. The ITS2 can be used as a “short barcode”. Among the advanced machine methods for delimitation species, the most effective algorithm for distinguishing <i>Coelastrella</i> species was the Generalized Mixed Yule Coalescent (GMYC) method. This paper represented for the first time our comprehensive review of the works devoted to the analysis of the biotechnological potential of representatives of the genus <i>Coelastrella</i> and shows that fatty acid composition of the three main chemogroups within the studied genus differs. In the future, this may form the basis for predicting the composition of the fatty acid profile of new strains, which is important while searching for organisms with specified biotechnological properties. In conclusion, an integrative approach was employed to describe <i>Coelastrella affinis</i> sp. nov., a new species of the genus <i>Coelastrella</i> with high biotechnological potential. Also, a new description of <i>C. thermophila</i> var. <i>astaxanthina</i> comb. nov. was proposed.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142001264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peteryoungia algae sp. nov. isolated from seaweeds of Gouqi Island, China, and its unique genetic features among Peteryoungia strains","authors":"Jun Yin,&nbsp;Min He,&nbsp;Xiao-Xiao Liu,&nbsp;Chang-Bin Ren,&nbsp;Hou-Hong Liu,&nbsp;Hai Luo,&nbsp;Gen Chen,&nbsp;Ze-Fei Wang,&nbsp;Sanjit Chandra Debnath,&nbsp;Pin-Mei Wang,&nbsp;Hai-Xin Chen,&nbsp;Dao-Qiong Zheng","doi":"10.1007/s10482-024-02010-7","DOIUrl":"10.1007/s10482-024-02010-7","url":null,"abstract":"<div><p>A Gram-stain-negative, light khaki, strictly aerobic, rod-shaped, motile via multiple flagella, and catalase- and oxidase-positive bacterium, designated as SSM4.3^T, was isolated from the seaweed of Gouqi Island in the East China Sea. The novel isolate grows at 0–5.0% NaCl concentrations (w/v) (optimum 1%), pH 5.0–9.0 (optimum pH 7.0), and 15–37 °C (optimum 30 °C). The 16S rRNA gene sequences-based phylogeny indicates that the novel marine isolate belongs to the family <i>Rhizobiaceae</i> and that it shared the greatest sequence similarity (98.9%) with <i>Peteryoungia rhizophila</i> CGMCC 1.15691^T. This classification was also supported by phylogenetic analysis using core genes. The predominant fatty acids (≥ 10%) of the strain were identified as C_18:1 ω7c/C_18:1 ω6c. Q-10 was identified as the major isoprenoid quinone, with trace levels of Q-9 present. The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The complete genome size of strain SSM4.3^T is 4.39 Mb with a DNA G+C content of 61.3%. The average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity values between the genomes of strain SSM4.3^T and its closely related representatives were 74.80–86.93%, 20.00–32.30%, and 70.30–91.52%, respectively. Phylogenetic analysis, grounded on the core genes, reveals the evolutionary relationship between SSM4.3^T and other <i>Peteryoungia</i> strains. Pan-genomics analysis of 8 previously classified <i>Peteryoungia</i> species and SSM4.3^T revealed their unique genetic features and functions. Overall, strain SSM4.3^T was considered to be a new species of the <i>Peteryoungia</i> genus; the name <i>Peteryoungia algae</i> sp. nov. has been proposed, with type strain SSM4.3^T (= LMG 32561 = MCCC 1K07170).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141917989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional genomics and taxonomic insights into heavy metal tolerant novel bacterium Brevibacterium metallidurans sp. nov. NCCP-602T isolated from tannery effluent in Pakistan","authors":"Sadia Manzoor,&nbsp;Saira Abbas,&nbsp;Sobia Zulfiqar,&nbsp;Hong-Chuan Wang,&nbsp;Min Xiao,&nbsp;Wen-Jun Li,&nbsp;Muhammad Arshad,&nbsp;Iftikhar Ahmed","doi":"10.1007/s10482-024-02006-3","DOIUrl":"10.1007/s10482-024-02006-3","url":null,"abstract":"<div><p>The strain designated NCCP-602^T was isolated from tannery effluent, and displayed aerobic, gram-positive, rod-shaped cells that were characterized by oxidase negative, catalase positive, and non-motile features. The most favourable growth conditions were observed at a temperature of 30°C, pH 7.0, and NaCl concentration of 1% (w/v). It tolerated heavy metals at high concentrations of chromium (3600 ppm), copper (3300 ppm), cadmium (3000 ppm), arsenic (1200 ppm) and lead (1500 ppm). The results of phylogenetic analysis, derived from sequences of the 16S rRNA gene, indicated the position of strain NCCP-602^T within genus <i>Brevibacterium</i> and showed that it was closely related to <i>Brevibacterium ammoniilyticum</i> JCM 17537^T. Strain NCCP-602^T formed a robust branch that was clearly separate from closely related taxa. A comparison of 16S rRNA gene sequence similarity and dDDH values between the closely related type strains and strain NCCP-602^T provided additional evidence supporting the classification of strain NCCP-602^T as a distinct novel genospecies. The polar lipid profile included diphosphatidylglycerol, glycolipid, phospholipids and amino lipids. MK-7 and MK-8 were found as the respiratory quinones, while anteiso-C_15:0, iso-C_15:0, iso-C_16:0, iso-C_17:0, and anteiso-C_17:0 were identified as the predominant cellular fatty acids (&gt; 10%). Considering the convergence of phylogenetic, phenotypic, chemotaxonomic, and genotypic traits, it is suggested that strain NCCP-602^T be classified as a distinct species <i>Brevibacterium metallidurans</i> sp. nov. within genus <i>Brevibacterium</i> with type strain NCCP-602^T (JCM 18882^T = CGMCC1.62055^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141894812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Additional new species of Xenodidymella from pasture-medicinal plants in Iran","authors":"Maryam Karimi,&nbsp;Mehdi Mehrabi-Koushki,&nbsp;Reza Farokhinejad,&nbsp;Siamak Beigi","doi":"10.1007/s10482-024-02007-2","DOIUrl":"10.1007/s10482-024-02007-2","url":null,"abstract":"<div><p><i>Xenodidymella</i> species have a wide range of hosts and can be found as pathogens and saprobes. In this study, two new species of <i>Xenodidymella</i> were found from leaf diseases of three pasture-medicinal plants in Ilam Province, in the west of Iran, and proposed here as <i>X. ilamica</i> and <i>X. scandicis</i> spp. nov. These species were identified based on morphological features and phylogenetic analyses of the internal transcribed spacer regions 1 &amp; 2 and 5.8S nrDNA (ITS), partial beta-tubulin gene (<i>tub2</i>), and partial RNA polymerase II second largest subunit (<i>rpb2</i>) gene. The four <i>Xenodidymella</i> strains isolated in this study were delimited into two sister clades, with the two isolates of <i>X. ilamica</i> from the leaf spot of <i>Colchicum speciosum</i> and <i>Ficaria kochii</i> and two isolates of <i>X. scandicis</i> from leaf blight of <i>Scandix pecten-veneris</i>. Morphologically, <i>X. scandicis</i> produces larger, ostiolate or poroid pycnidia in vitro, while pycnidia in the cultures of <i>X. ilamica</i> are non-ostiolate and smaller. Some pycnidia in old cultures of <i>X. scandicis</i> produce a neck, but a distinct neck in <i>X. ilamica</i> has not been observed. Moreover, three plants under study are new hosts for the genus <i>Xenodidymella</i>.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141861500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}