Jaincy N. Jayan, Young-Mi Lee, Ye-ji You, Sathiyaraj Srinivasan, Sang-Seob Lee
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According to the NCBI 16S ribosomal RNA sequences database the strain 3YJGBD-33<sup>T</sup> and 3YJGBD-35<sup>T</sup> showed the highest 16S rRNA gene similarity with <i>Robertkochia marina</i> CC-AMO-30D<sup>T</sup> (97.0% and 98.4%), <i>Robertkochia sediminum</i> 1368<sup>T</sup> (96.4% and 98.3%) and <i>Robertkochia solimangrovi</i> CL23<sup>T</sup> (96.0% and 96.5%). The major respiratory quinone is MK-6. The polar lipid profile consists of phosphatidylethanolamine, three aminolipids and two unidentified lipids. The major cellular fatty acids for strains 3YJGBD-33<sup>T</sup> and 3YJGBD-35<sup>T</sup> were iso-C<sub>15:0,</sub> iso-C<sub>15:1</sub> G and iso-C<sub>17:0</sub> 3-OH. The draft genome of strains 3YJGBD-33<sup>T</sup> and 3YJGBD-35<sup>T</sup> are 3.4 Mbp and 3.5 Mbp in size with 2993 coding genes, 41 tRNAs and 2935 coding genes, 42 tRNAs, respectively. The polyphasic analysis confirms that strains 3YJGBD-33<sup>T</sup> and 3YJGBD-35<sup>T</sup> belong to the genus <i>Robertkochia</i> and represent novel species, for which the names <i>Robertkochia aurantiaca</i> sp. nov. and <i>Robertkochia flava</i> sp. nov. are proposed. The type strains are 3YJGBD-33<sup>T</sup> (= KCTC 82715<sup>T</sup> = TBRC 19393<sup>T</sup>) and 3YJGBD-35<sup>T</sup> (= KCTC 82716<sup>T</sup> = JCM 34784<sup>T</sup>).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Robertkochia aurantiaca sp. nov., and Robertkochia flava sp. nov., two potential quinate- and catechol-degrading bacteria isolated from tidal-flat sediments\",\"authors\":\"Jaincy N. Jayan, Young-Mi Lee, Ye-ji You, Sathiyaraj Srinivasan, Sang-Seob Lee\",\"doi\":\"10.1007/s10482-025-02102-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Two Gram-negative-strains, 3YJGBD-33<sup>T</sup> and 3YJGBD-35<sup>T</sup> were isolated from tidal flat sediment in Daebudo, Republic of Korea. The isolates are non-motile, and long rods. Both strains are oxidase-positive and grew in the range of 15–45 °C (optimum, 40 °C) and pH 7–8 (optimum pH 8.0). Growth was observed in the salinity range of 1–6%, with optimum growth at 4.0% for strain 3YJGBD-33<sup>T</sup> and 3.0% for strain 3YJGBD-35<sup>T</sup>. According to the NCBI 16S ribosomal RNA sequences database the strain 3YJGBD-33<sup>T</sup> and 3YJGBD-35<sup>T</sup> showed the highest 16S rRNA gene similarity with <i>Robertkochia marina</i> CC-AMO-30D<sup>T</sup> (97.0% and 98.4%), <i>Robertkochia sediminum</i> 1368<sup>T</sup> (96.4% and 98.3%) and <i>Robertkochia solimangrovi</i> CL23<sup>T</sup> (96.0% and 96.5%). The major respiratory quinone is MK-6. The polar lipid profile consists of phosphatidylethanolamine, three aminolipids and two unidentified lipids. The major cellular fatty acids for strains 3YJGBD-33<sup>T</sup> and 3YJGBD-35<sup>T</sup> were iso-C<sub>15:0,</sub> iso-C<sub>15:1</sub> G and iso-C<sub>17:0</sub> 3-OH. The draft genome of strains 3YJGBD-33<sup>T</sup> and 3YJGBD-35<sup>T</sup> are 3.4 Mbp and 3.5 Mbp in size with 2993 coding genes, 41 tRNAs and 2935 coding genes, 42 tRNAs, respectively. The polyphasic analysis confirms that strains 3YJGBD-33<sup>T</sup> and 3YJGBD-35<sup>T</sup> belong to the genus <i>Robertkochia</i> and represent novel species, for which the names <i>Robertkochia aurantiaca</i> sp. nov. and <i>Robertkochia flava</i> sp. nov. are proposed. The type strains are 3YJGBD-33<sup>T</sup> (= KCTC 82715<sup>T</sup> = TBRC 19393<sup>T</sup>) and 3YJGBD-35<sup>T</sup> (= KCTC 82716<sup>T</sup> = JCM 34784<sup>T</sup>).</p></div>\",\"PeriodicalId\":50746,\"journal\":{\"name\":\"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology\",\"volume\":\"118 8\",\"pages\":\"\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2025-07-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10482-025-02102-y\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10482-025-02102-y","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Robertkochia aurantiaca sp. nov., and Robertkochia flava sp. nov., two potential quinate- and catechol-degrading bacteria isolated from tidal-flat sediments
Two Gram-negative-strains, 3YJGBD-33T and 3YJGBD-35T were isolated from tidal flat sediment in Daebudo, Republic of Korea. The isolates are non-motile, and long rods. Both strains are oxidase-positive and grew in the range of 15–45 °C (optimum, 40 °C) and pH 7–8 (optimum pH 8.0). Growth was observed in the salinity range of 1–6%, with optimum growth at 4.0% for strain 3YJGBD-33T and 3.0% for strain 3YJGBD-35T. According to the NCBI 16S ribosomal RNA sequences database the strain 3YJGBD-33T and 3YJGBD-35T showed the highest 16S rRNA gene similarity with Robertkochia marina CC-AMO-30DT (97.0% and 98.4%), Robertkochia sediminum 1368T (96.4% and 98.3%) and Robertkochia solimangrovi CL23T (96.0% and 96.5%). The major respiratory quinone is MK-6. The polar lipid profile consists of phosphatidylethanolamine, three aminolipids and two unidentified lipids. The major cellular fatty acids for strains 3YJGBD-33T and 3YJGBD-35T were iso-C15:0, iso-C15:1 G and iso-C17:0 3-OH. The draft genome of strains 3YJGBD-33T and 3YJGBD-35T are 3.4 Mbp and 3.5 Mbp in size with 2993 coding genes, 41 tRNAs and 2935 coding genes, 42 tRNAs, respectively. The polyphasic analysis confirms that strains 3YJGBD-33T and 3YJGBD-35T belong to the genus Robertkochia and represent novel species, for which the names Robertkochia aurantiaca sp. nov. and Robertkochia flava sp. nov. are proposed. The type strains are 3YJGBD-33T (= KCTC 82715T = TBRC 19393T) and 3YJGBD-35T (= KCTC 82716T = JCM 34784T).
期刊介绍：
Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.
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