Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology最新文献

{"title":"Methylobacterium nigriterrae sp. nov., isolated from black soil","authors":"Le-Bin Chen,&nbsp;Yu-Ting OuYang,&nbsp;Lan Liu,&nbsp;Pin-Jiao Jin,&nbsp;Rong-Rong Huang,&nbsp;Wen-Yi Pan,&nbsp;Ying Wang,&nbsp;Jia-Ying Xing,&nbsp;Ting-Ting She,&nbsp;Jian-Yu Jiao,&nbsp;Shuang Wang,&nbsp;Wen-Jun Li","doi":"10.1007/s10482-024-01981-x","DOIUrl":"10.1007/s10482-024-01981-x","url":null,"abstract":"<div><p>An aerobic, Gram-stain-negative, motile rod bacterium, designated as SYSU BS000021^T, was isolated from a black soil sample in Harbin, Heilongjiang province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus <i>Methylobacterium</i>, and showed the highest sequence similarity to <i>Methylobacterium segetis</i> KCTC 62267^T (98.51%) and <i>Methylobacterium oxalidis</i> DSM 24028^T (97.79%). Growth occurred at 20–37℃ (optimum, 28 °C), pH 6.0–8.0 (optimum, pH 7.0) and in the presence of 0% (w/v) NaCl. Polar lipids comprised of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminolipid and one unidentified polar lipid. The major cellular fatty acids (&gt; 5%) were C_18:0 and C_18:1 <i>ω</i>7<i>c</i> and/or C_18:1 <i>ω</i>6<i>c</i>. The predominant respiratory quinone was Q-10. The genomic G + C content was 68.36% based on the whole genome analysis. The average nucleotide identity (≤ 83.5%) and digital DNA–DNA hybridization (≤ 27.3%) values between strain SYSU BS000021^T and other members of the genus <i>Methylobacterium</i> were all lower than the threshold values recommended for distinguishing novel prokaryotic species. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain SYSU BS000021^T represents a novel species of the genus <i>Methylobacterium</i>, for which the name <i>Methylobacterium nigriterrae</i> sp. nov. is proposed. The type strain of the proposed novel species is SYSU BS000021^T (= GDMCC 1.3814^T = KCTC 8051^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological potential of actinomycetes in the 21st century: a brief review","authors":"Rafael de Souza Rodrigues,&nbsp;Antonia Queiroz Lima de Souza,&nbsp;Maria Divina Oliveira Feitoza,&nbsp;Thalita Caroline Lima Alves,&nbsp;Anderson Nogueira Barbosa,&nbsp;Sarah Raquel Silveira da Silva Santiago,&nbsp;Afonso Duarte Leão de Souza","doi":"10.1007/s10482-024-01964-y","DOIUrl":"10.1007/s10482-024-01964-y","url":null,"abstract":"<div><p>This brief review aims to draw attention to the biotechnological potential of actinomycetes. Their main uses as sources of antibiotics and in agriculture would be enough not to neglect them; however, as we will see, their biotechnological application is much broader. Far from intending to exhaust this issue, we present a short survey of the research involving actinomycetes and their applications published in the last 23 years. We highlight a perspective for the discovery of new active ingredients or new applications for the known metabolites of these microorganisms that, for approximately 80 years, since the discovery of streptomycin, have been the main source of antibiotics. Based on the collected data, we organize the text to show how the cosmopolitanism of actinomycetes and the evolutionary biotic and abiotic ecological relationships of actinomycetes translate into the expression of metabolites in the environment and the richness of biosynthetic gene clusters, many of which remain silenced in traditional laboratory cultures. We also present the main strategies used in the twenty-first century to promote the expression of these silenced genes and obtain new secondary metabolites from known or new strains. Many of these metabolites have biological activities relevant to medicine, agriculture, and biotechnology industries, including candidates for new drugs or drug models against infectious and non-infectious diseases. Below, we present significant examples of the antimicrobial spectrum of actinomycetes, which is the most commonly investigated and best known, as well as their non-antimicrobial spectrum, which is becoming better known and increasingly explored.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141094416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paracoccus benzoatiresistens sp. nov., a benzoate resistance and selenite reduction bacterium isolated from wetland","authors":"Weidong Mu,&nbsp;Haoran Liu,&nbsp;Bai Guo,&nbsp;Kaiyue Wang,&nbsp;Jinhua Hu,&nbsp;Jianjun Song,&nbsp;Xiuyun Li,&nbsp;Shuzhen Wei,&nbsp;Aijv Liu,&nbsp;Hongliang Liu","doi":"10.1007/s10482-024-01969-7","DOIUrl":"10.1007/s10482-024-01969-7","url":null,"abstract":"&lt;div&gt;&lt;p&gt;A Gram-stain-negative, aerobic, non-motile, catalase- and oxidase-positive, pale orange, rod-shaped strain EF6&lt;sup&gt;T&lt;/sup&gt;, was isolated from a natural wetland reserve in Hebei province, China. The strain grew at 25–37 °C (optimum, 30 °C), pH 5–9 (optimum, pH 7), and in the presence of 1.0–4.0% (w/v) NaCl (optimum, 2%). A phylogenetic analysis based on 16S rRNA gene sequence revealed that strain EF6&lt;sup&gt;T&lt;/sup&gt; belongs to the genus &lt;i&gt;Paracoccus&lt;/i&gt;, and the closest members were &lt;i&gt;Paracoccus shandongensis&lt;/i&gt; wg2&lt;sup&gt;T&lt;/sup&gt; with 98.1% similarity, &lt;i&gt;Paracoccus fontiphilus&lt;/i&gt; MVW-1&lt;sup&gt; T&lt;/sup&gt; (97.9%), &lt;i&gt;Paracoccus everestensis&lt;/i&gt; S8-55&lt;sup&gt; T&lt;/sup&gt; (97.7%), &lt;i&gt;Paracoccus subflavus&lt;/i&gt; GY0581&lt;sup&gt;T&lt;/sup&gt; (97.6%), &lt;i&gt;Paracoccus sediminis&lt;/i&gt; CMB17&lt;sup&gt;T&lt;/sup&gt; (97.3%), &lt;i&gt;Paracoccus caeni&lt;/i&gt; MJ17&lt;sup&gt;T&lt;/sup&gt; (97.0%), and &lt;i&gt;Paracoccus angustae&lt;/i&gt; E6&lt;sup&gt;T&lt;/sup&gt; (97.0%). The genome size of strain EF6&lt;sup&gt;T&lt;/sup&gt; was 4.88 Mb, and the DNA G + C content was 65.3%. The digital DNA-DNA hybridization, average nucleotide identity, and average amino acid identity values between strain EF6&lt;sup&gt;T&lt;/sup&gt; and the reference strains were all below the threshold limit for species delineation (&lt; 32.8%, &lt; 88.0%, and &lt; 86.7%, respectively). The major fatty acids (≥ 5.0%) were summed feature 8 (86.3%, C&lt;sub&gt;18:1&lt;/sub&gt; &lt;i&gt;ω&lt;/i&gt;6&lt;i&gt;c&lt;/i&gt; and/or C&lt;sub&gt;18:1&lt;/sub&gt; &lt;i&gt;ω&lt;/i&gt;7&lt;i&gt;c&lt;/i&gt;) and C&lt;sub&gt;18:1&lt;/sub&gt; (5.0%) and the only isoprenoid quinone was Q-10. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids, five unidentified phospholipids, and an unidentified aminolipid. Strain EF6&lt;sup&gt;T&lt;/sup&gt; displays notable resistance to benzoate and selenite, with higher tolerance levels (25 g/L for benzoate and 150 mM for selenite) compared to the closely related species. Genomic analysis identified six benzoate resistance genes (&lt;i&gt;acdA&lt;/i&gt;, &lt;i&gt;pcaF&lt;/i&gt;, &lt;i&gt;fadA&lt;/i&gt;, &lt;i&gt;pcaC&lt;/i&gt;, &lt;i&gt;purB&lt;/i&gt;, and &lt;i&gt;catA&lt;/i&gt;) and twenty selenite resistance and reduction-related genes (&lt;i&gt;iscR&lt;/i&gt;, &lt;i&gt;ssuB&lt;/i&gt;, &lt;i&gt;ssuD&lt;/i&gt;, &lt;i&gt;selA&lt;/i&gt;, &lt;i&gt;selD&lt;/i&gt; and so on). Additionally, EF6&lt;sup&gt;T&lt;/sup&gt; possesses unique genes (&lt;i&gt;catA&lt;/i&gt;, &lt;i&gt;ssuB&lt;/i&gt;, and &lt;i&gt;ssuC&lt;/i&gt;) absent in the closely related species for benzoate and selenite resistance. Its robust resistance to benzoate and selenite, coupled with its genomic makeup, make EF6&lt;sup&gt;T&lt;/sup&gt; a promising candidate for the remediation of both organic and inorganic pollutants. It is worth noting that the specific resistance phenotypes described above were not reported in other novel species in &lt;i&gt;Paracoccus&lt;/i&gt;. Based on the results of biochemical, physiological, phylogenetic, and chemotaxonomic analyses, combined with comparisons of the 16S rRNA gene sequence and the whole genome sequence, strain EF6&lt;sup&gt;T&lt;/sup&gt; is considered to represent a novel species of the genus &lt;i&gt;Paracoccus&lt;/i&gt; within the family &lt;i&gt;Rhodobacteraceae&lt;/i&gt;, for which the name &lt;i&gt;Paracoccus benzoatiresistens&lt;/i&gt; sp. nov. is p","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141080987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adlercreutzia faecimuris sp. nov., producing propionate and acetate isolated from mouse feces","authors":"Min Kuk Suh,&nbsp;Jong- Sik Jin,&nbsp;Hyo Eun Do,&nbsp;Ji-Sun Kim,&nbsp;Mi Kyung Eom,&nbsp;Han Sol Kim,&nbsp;Jung- Sook Lee","doi":"10.1007/s10482-024-01980-y","DOIUrl":"10.1007/s10482-024-01980-y","url":null,"abstract":"<div><p>A novel strictly anaerobic bacterium, strain JBNU-10^T, was isolated from BALB/c mouse feces. Cells of the strain JBNU-10^T were Gram-stain positive, non-motile and rod-shaped. Optimum growth occurred at 37℃, with 1% (w/v) NaCl and at pH 7. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain JBNU-10^T belonged to the genus <i>Adlercreutzia</i> and were closely related to <i>Adlercreutzia muris</i> WCA-131-CoC-2^T (95.90%). The genome sequencing of strain JBNU-10^T revealed a genome size of 2,790,983 bp, a DNA G + C content of 69.4 mol%. It contains a total of 2,266 CDSs, 5 rRNA genes and 49 tRNA genes. According to the data obtained strain JBNU-10^T shared ANI value below 77.6- 67.7%, dDDH value below 23.8% with the closely type species. Strain JBNU-10^T possessed iso-C_16:0 DMA, C_18:1 CIS 9 FAME, and C_18:0 DMA as the major fatty acids and had DMMK-6. The major end products of fermentation is propionate and acetate. Based on phylogenetic, physiological and chemotaxonomic characteristics, strain JBNU-10^T represent a novel species of the genus <i>Adlercreutzia</i>. The type strain is JBNU-10^T (= KCTC 25028^T = CCUG 75610^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141077256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Azotosporobacter soli gen. nov., sp. nov., a novel nitrogen-fixing bacterium isolated from paddy soil","authors":"Cheng-Jie Xie,&nbsp;Ling Yao,&nbsp;Rong Tang,&nbsp;Shuang Han,&nbsp;Shang Yang,&nbsp;Hend Alwathnani,&nbsp;Christopher Rensing,&nbsp;Guo-Hong Liu,&nbsp;Shun-Gui Zhou","doi":"10.1007/s10482-024-01978-6","DOIUrl":"10.1007/s10482-024-01978-6","url":null,"abstract":"<div><p>A nitrogen-fixing strain designated SG130^T was isolated from paddy soil in Fujian Province, China. Strain SG130^T was Gram-staining-negative, rod-shaped, and strictly anaerobic. Strain SG130^T showed the highest 16S rRNA gene sequence similarities with the type strains <i>Dendrosporobacter quercicolus</i> DSM 1736^T (91.7%), <i>Anaeroarcus burkinensis</i> DSM 6283^T (91.0%) and <i>Anaerospora hongkongensis</i> HKU 15^T (90.9%). Furthermore, the phylogenetic and phylogenomic analysis also suggested strain SG130^T clustered with members of the family <i>Sporomusaceae</i> and was distinguished from other genera within this family. Growth of strain SG130^T was observed at 25–45 °C (optimum 30 °C), pH 6.0–9.5 (optimum 7.0) and 0–1% (w/v) NaCl (optimum 0.1%). The quinones were Q-8 and Q-9. The polar lipids were phosphatidylserine (PS), phosphatidylethanolamine (PE), glycolipid (GL), phospholipid (PL) and an unidentified lipid (UL). The major fatty acids (&gt; 10%) were iso-C_13:0 3OH (26.6%), iso-C_17:1 (15.6%) and iso-C_15:1 F (11.4%). The genomic DNA G + C content was 50.7%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain SG130^T and the most closely related type strain <i>D</i>. <i>quercicolus</i> DSM 1736^T (ANI 68.0% and dDDH 20.3%) were both below the cut-off level for species delineation. The average amino acid identity (AAI) between strain SG130^T and the most closely related type strain <i>D</i>. <i>quercicolus</i> DSM 1736^T was 63.2%, which was below the cut-off value for bacterial genus delineation (65%). Strain SG130^T possessed core genes (<i>nifHDK</i>) involved in nitrogen fixation, and nitrogenase activity (106.38 μmol C₂H₄ g⁻¹ protein h⁻¹) was examined using the acetylene reduction assay. Based on the above results, strain SG130^T is confirmed to represent a novel genus of the family <i>Sporomusaceae</i>, for which the name <i>Azotosporobacter soli</i> gen. nov., sp. nov. is proposed. The type strain is SG130^T (= GDMCC 1.3312^T = JCM 35641^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140960139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling antibiofilm potential: proteins from Priestia sp. targeting Staphylococcus aureus biofilm formation","authors":"Nicole Sartori Ribeiro,&nbsp;Deisiane Fernanda da Rosa,&nbsp;Marina Amaral Xavier,&nbsp;Sharon Vieira dos Reis,&nbsp;Walter O. Beys-da-Silva,&nbsp;Lucélia Santi,&nbsp;Cristiano Valim Bizarro,&nbsp;Pedro Ferrari Dalberto,&nbsp;Luiz Augusto Basso,&nbsp;Alexandre José Macedo","doi":"10.1007/s10482-024-01977-7","DOIUrl":"10.1007/s10482-024-01977-7","url":null,"abstract":"<div><p><i>Staphylococcus aureus</i> is the etiologic agent of many nosocomial infections, and its biofilm is frequently isolated from medical devices. Moreover, the dissemination of multidrug-resistant (MDR) strains from this pathogen, such as methicillin-resistant <i>S. aureus</i> (MRSA) strains, is a worldwide public health issue. The inhibition of biofilm formation can be used as a strategy to weaken bacterial resistance. Taking that into account, we analysed the ability of marine sponge-associated bacteria to produce antibiofilm molecules, and we found that marine <i>Priestia</i> sp., isolated from marine sponge <i>Scopalina</i> sp. collected on the Brazilian coast, secretes proteins that impair biofilm development from <i>S. aureus</i>. Partially purified proteins (PPP) secreted after 24 hours of bacterial growth promoted a 92% biofilm mass reduction and 4.0 µg/dL was the minimum concentration to significantly inhibit biofilm formation. This reduction was visually confirmed by light microscopy and Scanning Electron Microscopy (SEM). Furthermore, biochemical assays showed that the antibiofilm activity of PPP was reduced by ethylenediaminetetraacetic acid (EDTA) and 1,10 phenanthroline (PHEN), while it was stimulated by zinc ions, suggesting an active metallopeptidase in PPP. This result agrees with mass spectrometry (MS) identification, which indicated the presence of a metallopeptidase from the M28 family. Additionally, whole-genome sequencing analysis of <i>Priestia</i> sp. shows that gene <i>ywad</i>, a metallopeptidase-encoding gene, was present. Therefore, the results presented herein indicate that PPP secreted by the marine <i>Priestia</i> sp. can be explored as a potential antibiofilm agent and help to treat chronic infections.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140917361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endophytic species of Nigrospora from grasses and shrubs of Shadegan International Wetland, with new species and records from Iran","authors":"Atena Safi,&nbsp;Mehdi Mehrabi-Koushki,&nbsp;Mahdi Arzanlou","doi":"10.1007/s10482-024-01976-8","DOIUrl":"10.1007/s10482-024-01976-8","url":null,"abstract":"<div><p>The “Shadegan International Wetland” (SIW) is one of the wetlands internationally recognized in the Ramsar convention. The vegetation of this wetland ecosystem consists of mostly grasses and shrubs that host a large number of fungi including endophytes. In this study, <i>Nigrospora</i> isolates were obtained from healthy plants of this wetland and its surrounding salt marshes and identified based on morphological features and multilocus phylogenetic analyses based on three DNA loci, namely the internal transcribed spacer regions 1 and 2 including the intervening 5.8S nuclear ribosomal DNA (ITS), β-tubulin (<i>tub2</i>), and elongation factor 1-α (<i>tef1-α</i>). Accordingly, the following <i>Nigrospora</i> species were identified: <i>N. lacticolonia</i>, <i>N. oryzae</i>, <i>N. osmanthi</i>, <i>N. pernambucoensis</i> and a novel taxon <i>N. shadeganensis</i> sp. nov., which is described and illustrated. To the best of our knowledge, 10 new hosts for <i>Nigrospora</i> species are here reported, namely <i>Aeluropus lagopoides</i><i>, </i><i>Allenrolfea occidentalis</i><i>, </i><i>Anthoxanthum monticola</i>, <i>Arthrocnemum macrostachyum</i><i>, </i><i>Cressa cretica</i><i>, </i><i>Halocnemum strobilaceum</i><i>, </i><i>Seidlitzia rosmarinus</i><i>, </i><i>Suaeda vermiculata</i><i>, </i><i>Tamarix passerinoides,</i> and <i>Typha latifolia</i>. Moreover, the species <i>N. lacticolonia</i> and <i>N. pernambucoensis</i> are new records for the mycobiota of Iran.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140877855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative genomics of plant growth promoting phosphobacteria isolated from acidic soils","authors":"Carlos Cortés-Albayay,&nbsp;Mabel Delgado-Torres,&nbsp;Giovanni Larama,&nbsp;Cecilia Paredes-Negron,&nbsp;María de la Luz Mora,&nbsp;Paola Durán,&nbsp;Patricio Javier Barra","doi":"10.1007/s10482-024-01961-1","DOIUrl":"10.1007/s10482-024-01961-1","url":null,"abstract":"<div><p>Despite being one of the most abundant elements in soil, phosphorus (P) often becomes a limiting macronutrient for plants due to its low bioavailability, primarily locked away in insoluble organic and inorganic forms. Phosphate solubilizing and mineralizing bacteria, also called phosphobacteria, isolated from P-deficient soils have emerged as a promising biofertilizer alternative, capable of converting these recalcitrant P forms into plant-available phosphates. Three such phosphobacteria strains—<i>Serratia</i> sp. RJAL6, <i>Klebsiella</i> sp. RCJ4, and <i>Enterobacter</i> sp. 198—previously demonstrated their particular strength as plant growth promoters for wheat, ryegrass, or avocado under abiotic stresses and P deficiency. Comparative genomic analysis of their draft genomes revealed several genes encoding key functionalities, including alkaline phosphatases, isonitrile secondary metabolites, enterobactin biosynthesis and genes associated to the production of indole-3-acetic acid (IAA) and gluconic acid. Moreover, overall genome relatedness indexes (OGRIs) revealed substantial divergence between <i>Serratia</i> sp. RJAL6 and its closest phylogenetic neighbours, <i>Serratia nematodiphila</i> and <i>Serratia bockelmanii</i>. This compelling evidence suggests that RJAL6 merits classification as a novel species. This in silico genomic analysis provides vital insights into the plant growth-promoting capabilities and provenance of these promising PSRB strains. Notably, it paves the way for further characterization and potential application of the newly identified <i>Serratia</i> species as a powerful bioinoculant in future agricultural settings.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140867145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation of biogenic fluorescent pigmented PHB nanoparticles from Rhodanobacter sp. for drug delivery","authors":"Ksheerabdhi Tanaya,&nbsp;Swati Mohapatra,&nbsp;Deviprasad Samantaray","doi":"10.1007/s10482-024-01973-x","DOIUrl":"10.1007/s10482-024-01973-x","url":null,"abstract":"<div><p>Biogenic nanoparticles (NPs) have emerged as promising therapeutic formulations in effective drug delivery. Despite of various positive attributes, these NPs are often conjugated with various cytotoxic organic fluorophores for bioimaging, thereby reducing its effectiveness as a potential carrier. Herein, we aim to formulate biogenic fluorescent pigmented polyhydroxybutyrate (PHB) NPs from <i>Rhodanobacter</i> sp. strain KT31 (OK001852) for drug delivery. The bacterial strain produced 0.5 g L⁻¹ of polyhydroxyalkanoates (PHAs) from 2.04 g L⁻¹ of dry cell weight (DCW) under optimised conditions via submerged fermentation. Further, structural, thermal, and morphological charactersiation of the extracted PHAs was conducted using advance analytical technologies. IR spectra at 1719.25 cm⁻¹ confirmed presence of C = O functional group PHB. NMR and XRD analysis validated the chemical structure and crystallinity of PHB. TG–DTA revealed <i>Tm</i> (168 °C), <i>Td</i> (292 °C), and <i>Xc</i> (35%) of the PHB. FE-SEM imaging indicated rough surface of the PHB film and the biodegradability was confirmed from open windro composting. WST1 assay showed no significant cell death (&gt; 50%) from 100 to 500 µg/mL, endorsing non-cytotoxic nature of PHB. PHB NPs were uniform, smooth and spherical with size distribution and mean zeta potential 44.73 nm and 0.5 mV. IR and XRD peaks obtained at 1721.75 cm⁻¹ and 48.42 Å denoted C = O and crystalline nature of PHB. Cell proliferation rate of PHB NPs was quite significant at 50 µg/mL, establishing the non-cytotoxic nature of NPs. Further, in vitro efficacy of the PHB NPs needs to be evaluated prior to the biomedical applications.</p><h3>Graphical Abstract</h3><p>Schematic diagram depicting synthesis and characterisation of fluorescent PHB NPs</p>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140861327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jeotgalibacillus haloalkalitolerans sp. nov., a novel alkalitolerant and halotolerant bacterium, isolated from the confluence of the Fenhe River and the Yellow River","authors":"Ya-Fei Zhang,&nbsp;Yun-Lin Peng,&nbsp;Yong-Hui Xiao,&nbsp;Bing Yu,&nbsp;Ming-Yue Cao,&nbsp;Zhi-Ling Zhang,&nbsp;Le-Qun Huang,&nbsp;Yi Li","doi":"10.1007/s10482-024-01968-8","DOIUrl":"10.1007/s10482-024-01968-8","url":null,"abstract":"<div><p>A Gram-stain positive, aerobic, alkalitolerant and halotolerant bacterium, designated HH7-29^T, was isolated from the confluence of the Fenhe River and the Yellow River in Shanxi Province, PR China. Growth occurred at pH 6.0–12.0 (optimum, pH 8.0–8.5) and 15–40℃ (optimum, 32℃) with 0.5–24% NaCl (optimum, 2–9%). The predominant fatty acids (&gt; 10.0%) were iso-C_15:0 and anteiso-C_15:0. The major menaquinones were MK-7 and MK-8. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified phospholipids. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that strain HH7-29^T was a member of the genus <i>Jeotgalibacillus</i>, exhibiting high sequence similarity to the 16S rRNA gene sequences of <i>Jeotgalibacillus alkaliphilus</i> JC303^T (98.4%), <i>Jeotgalibacillus salarius</i> ASL-1^T (98.1%) and <i>Jeotgalibacillus alimentarius</i> YKJ-13^T (98.1%). The genomic DNA G + C content was 43.0%. Gene annotation showed that strain HH7-29^T had lower protein isoelectric points (pIs) and possessed genes related to ion transport and organic osmoprotectant uptake, implying its potential tolerance to salt and alkali. The average nucleotide identity, digital DNA–DNA hybridization values, amino acid identity values, and percentage of conserved proteins values between strain HH7-29^T and its related species were 71.1–83.8%, 19.5–27.4%, 66.5–88.4% and 59.8–76.6%, respectively. Based on the analyses of phenotypic, chemotaxonomic, phylogenetic and genomic features, strain HH7-29^T represents a novel species of the genus <i>Jeotgalibacillus</i>, for which the name <i>Jeotgalibacillus haloalkalitolerans</i> sp. nov. is proposed. The type strain is HH7-29^T (= KCTC 43417^T = MCCC 1K07541^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140828357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}