Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology最新文献

{"title":"Unveiling the influence of autochthonous yeasts selected from spontaneous fermentation of cachaça on ethanol, ester and fermentation kinetics","authors":"Luanna Maia,&nbsp;Levi Felizardo,&nbsp;Maria Clara De-Barros,&nbsp;Júlia Bizerra-Santos,&nbsp;Flávio Luiz Honorato da Silva,&nbsp;Normando Ribeiro-Filho","doi":"10.1007/s10482-025-02123-7","DOIUrl":"10.1007/s10482-025-02123-7","url":null,"abstract":"<div><p>Autochthonous yeasts from cachaça productions are not well known compared to industrial <i>Saccharomyces cerevisiae</i> strains. Although yeast diversity some in spontaneous sugarcane fermentations have been examined, their metabolic roles, adaptation mechanisms, and effects on ethanol and aroma formation remain largely unexplored. This study aims to investigate the influence of autochthonous yeasts selected from the spontaneous fermentations of cachaça on ethanol, ester and fermentation kinetics, evaluating their metabolic diversity and potential contribution to the aromatic complexity of the beverage. Fermentations were conducted in synthetic must (15°Brix) with yeast concentration (YC) levels of 90 × 10⁷, 120 × 10⁷, and 150 × 10⁷ cells/mL at 30 °C for 24 h. Higher YC accelerated fermentation but reduced ethanol and ester formation, while lower YC enhanced ethanol and ester production with lower acidity. PCA revealed significant correlations between physicochemical and kinetic parameters, indicating that alkaline conditions favor alcohol and ester production. These findings improve fermentation control, contribute to yeast strain databases, and support the sugar-alcohol industry as part of Paraíba’s and Brazil’s cultural and economic heritage.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipolytic activity of carotenogenic yeast isolated from the polish ecosystem","authors":"Anna M. Kot,&nbsp;Paweł Buczyński,&nbsp;Katarzyna Pobiega,&nbsp;Iwona Gientka,&nbsp;Stanisław Błażejak","doi":"10.1007/s10482-025-02115-7","DOIUrl":"10.1007/s10482-025-02115-7","url":null,"abstract":"<div><p>The production of lipolytic enzymes by 130 red yeast strains isolated from various environments in Poland was assessed. Based on two screening tests on Petri dishes, 12 yeast strains with the highest potential lipolytic activity were selected. All strains belonged to the genus <i>Rhodotorula</i>. Further analysis showed that the most efficient producer of extracellular lipases was <i>Rhodotorula babjevae</i> A-110 (CMIFS 163), with lipolytic activity ranging from 39.11 to 50.35 U/mL during batch cultures in flasks. After cultivating this strain in a 5-L laboratory bioreactor, the highest lipase activity (137.25 U/mL) was achieved after 48 h in a medium with sunflower oil. The Michaelis–Menten constant of the lipases was 4.55 × 10⁻⁴ M. Maximum activity was observed at pH 7.5–8.0, with an optimal temperature of 37 °C at pH 8.0. Lipolytic enzymes were stable at 20, 30, and 40 °C, but butanol completely inactivated <i>R. babjevae</i> lipases. Following protein precipitation from the postculture liquid with ammonium sulfate, lipolytic activity reached 352.52 U/mL, 2.5-fold higher than in the supernatant after cultivation of <i>R. babjevae</i> in a bioreactor.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12228664/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144568045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isoptericola rhizosphaerae sp. nov. and Isoptericola aurantiacus sp. nov. two novel bacteria isolated from rhizosphere soil","authors":"Haifei Liu,&nbsp;Quan Yang,&nbsp;Jiawei Li,&nbsp;Fei Yang,&nbsp;Caifei Li,&nbsp;Ding Li,&nbsp;Ying Sun,&nbsp;Lifang Yang,&nbsp;Mingguo Jiang","doi":"10.1007/s10482-025-02105-9","DOIUrl":"10.1007/s10482-025-02105-9","url":null,"abstract":"<div><p>Two actinobacterial strains, designated GXG3202^T and GXG9306^T, were isolated from the rhizosphere soil of <i>Avicennia marina</i> in Beihai, Guangxi Zhuang Autonomous Region, China. Phylogenetic analysis of the 16S rRNA gene sequence revealed that both strains clustered in distinct subclades in the genus <i>Isoptericola</i>, sharing high sequence similarity with <i>Isoptericola halotolerans</i> YIM 70177^T (98.8% and 97.9%) and <i>Isoptericola chiayiensis</i> 06182M-1^T (98.4% and 98.6%). Chemotaxonomic analysis indicated that the predominant fatty acids are <i>anteiso</i>-C_15:0, C_16:0, and <i>anteiso</i>-C_17:0. The peptidoglycan hydrolysate contained lysine and aspartate, with the menaquinone MK-9(H₂) and MK-9(H₄) identified as the major respiratory quinone. Polar lipid analysis identified DPG, PGL, PI, and GL as core components in both strains, with GXG3202^T uniquely containing two PLs and PG, whereas GXG9306^T contained one PL. The genomic DNA G + C content of GXG3202^T and GXG9306^T were determined to be 72.4% and 73.1%, respectively. Digital DNA-DNA hybridization (dDDH) values between the novel strains and species of genus <i>Isoptericola</i> ranged from 22.2 to 44.1%, while average nucleotide identity (ANI) and amino acid identity (AAI) values were 71.0–83.6% and 74.3–83.8%, respectively. Our collective taxonomic evidence supports the classification of these strains as two novel species within the genus <i>Isoptericola</i>, for which the names <i>Isoptericola rhizosphaerae</i> sp. nov.(type strain GXG3202^T = MCCC 1K09564^T = KCTC 59469^T) and <i>Isoptericola aurantiacus</i> sp. nov. (type strain GXG9306^T = MCCC 1K09565^T = KCTC59470^T) are proposed, respectively.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144545924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flavobacterium aestuarii sp. nov., a novel species isolated from the Pearl River Estuary","authors":"Qi Li,&nbsp;Zi-Qi Peng,&nbsp;Hong-Chuan Wang,&nbsp;Lei Dong,&nbsp;Dan-Yuan Guo,&nbsp;Pan-Deng Wang,&nbsp;Xiao-Qing Luo,&nbsp;Zhen-Hao Luo,&nbsp;Zi-Wen Yang,&nbsp;Wen-Jun Li","doi":"10.1007/s10482-025-02109-5","DOIUrl":"10.1007/s10482-025-02109-5","url":null,"abstract":"<div><p>Strain SYSU M80003^T, a Gram-stain-negative and aerobic bacterium, was isolated from water sample of the Pearl River Estuary, Guangzhou, Guangdong, PR China. The strain could grow at 4–37 °C (optimal: 28 °C), pH 6.0–8.0 (optimal: 7.0), and with 0–1% NaCl (w/v, optimal: 0%). The main fatty acids were anteiso-C_14:0, Summed Feature 3 (C_16:1 <i>ω</i>7<i>c</i> and/or C_16:1 <i>ω</i>6<i>c</i>) and iso-C_15:0. The predominant respiratory quinone was MK-6. The polar lipids contained phosphatidylethanolamine, three unidentified aminolipids, two unidentified aminophospholipids, and two unidentified polar lipids. The genomic DNA G + C content was 35.06%. Phylogenetic analysis based on the 16S rRNA gene revealed that strain SYSU M80003^T was closely related to <i>Flavobacterium aquicola</i> JCM 30987^T with similarity of 97.28%. Furthermore, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain SYSU M80003^T and <i>F. aquicola</i> JCM 30987^T were 83.8% and 28.1%, respectively. Based upon the consensus of phylogenetic and ANI analyses, we conclude that strain SYSU M80003^T represents a novel species within the genus <i>Flavobacterium</i>, for which the name <i>Flavobacterium aestuarii</i> sp. nov. is proposed. The type strain is SYSU M80003^T (= GDMCC 1.4521^T = KCTC 102267^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144545922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stress-induced proteins enhance thermal endurance in soil bacterium Priestia aryabhattai strain PSK.N2","authors":"Nagarjuna Prakash Dalbanjan,&nbsp;Arihant Jayawant Kadapure,&nbsp;S. K. Praveen Kumar","doi":"10.1007/s10482-025-02118-4","DOIUrl":"10.1007/s10482-025-02118-4","url":null,"abstract":"<div><p>Soil microbial communities play vital roles in ecological stability and offer significant potential for biotechnological innovations. These microorganisms frequently encounter abiotic stressors such as heat, which can activate protective mechanisms including the overexpression of heat shock proteins (HSPs). In this study, a resilient soil bacterium, <i>Priestia aryabhattai</i> strain PSK.N2, was isolated from the Western Ghats of India and identified through a polyphasic approach. Growth parameters were optimized before subjecting the strain to heat stress, both alone and in the presence of sub-lethal concentrations of antibiotics that target distinct cellular processes; DNA replication, RNA transcription, protein synthesis, and cell wall biosynthesis. Comparative protein profiling (via SDS-PAGE), protein aggregation assays, and survival assessments (single spot dilution and CFU enumeration) revealed that heat-stressed cells showed enhanced thermal tolerance compared to those grown under optimal conditions. This increased endurance was associated with the transient overexpression of ~ 17–90 kDa stress-related proteins. In contrast, cells treated with transcription and translation-inhibiting antibiotics (rifampicin and chloramphenicol) showed diminished tolerance, likely due to impaired synthesis of stress-response proteins. These findings suggest that pre-exposure to stress enhances cellular preparedness through selective protein induction, offering insights for developing stress-resilient microbial strains for applications in bioprocessing, environmental remediation, and microbial therapeutics.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144545925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and characterization of Polaribacter sargassicola sp. nov. and Algibacter ulvanivorans sp. nov.: two novel species with ability to degrade algal polysaccharides","authors":"Jin Li,&nbsp;Aihua Mao,&nbsp;Chao Peng,&nbsp;Linhui Li,&nbsp;Binghan Li,&nbsp;Lijuan Zhou,&nbsp;Yan Xu,&nbsp;Mingqi Zhong,&nbsp;Zhong Hu","doi":"10.1007/s10482-025-02117-5","DOIUrl":"10.1007/s10482-025-02117-5","url":null,"abstract":"<div><p>The genera <i>Polaribacter</i> and <i>Algibacter</i>, which are members of the Bacteroidota family Flavobacteriaceae, are well known for their arsenal of carbohydrate-active enzymes (CAZymes) and specialized polysaccharide utilization loci (PULs). In this study, two aerobic, gram-negative, and rod-shaped bacterial strains, designated strains DS7-9^T and PT7-4^T, were isolated from the surface of seaweed collected from the coastal zone of Fujian Province, China. The two strains exhibited broad tolerance ranges to salinity (0–4%), temperature (10–40 °C) and pH (5.0–10.0). The predominant fatty acids in strain DS7-9^T were iso-C_15:0 (53.7%) and iso-C_15:0 3-OH (10.9%), whereas those in strain PT7-4^T were iso-G-C_15:1 (33.2%) and iso-C_15:0 (27.1%). Additionally, the predominant menaquinone was MK-6. The 16S rRNA gene of strain DS7-9^T shares 97.5% similarity with that of <i>Polaribacter haliotis</i> KCTC 52418^T, whereas that of strain PT7-4^T is 97.0% sequence similarity with that of <i>Algibacter mikhailovii</i> KCTC 12710^T. Phylogenomic analysis revealed that the highest average nucleotide identity (ANI) value between strain DS7-9^T and its closest relative reached 84.7%, whereas the corresponding value for strain PT7-4^T was 84.2%. Genomic characterization revealed DNA G + C contents of 29.5% and 31.9% for strains DS7-9^T and PT7-4^T, respectively. Both strains possessed complete circular chromosomes, with genome sizes of 3.7 Mb (DS7-9^T) and 3.5 Mb (PT7-4^T). Bioinformatic analysis revealed that strain DS7-9^T harbors 5 PULs putatively associated with laminarin, ulvan, fucoidan, and alginate catabolism, whereas strain PT7-4^T possesses 7 PULs predicted to target ulvan, alginate, and starch metabolism. On the basis of their distinct physiological characteristics, two strains are suggested for classification into novel species: <i>Polaribacter sargassicola</i> sp. nov. (type strain DS7-9^T = MCCC 1K08670^T = KCTC 102076^T) and <i>Algibacter ulvanivorans</i> sp. nov. (type strain PT7-4^T = MCCC 1K08669^T = KCTC 102077^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144545923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of Paenibacillus jiagnxiensis sp. nov., a mesotrione-degrading bacterium isolated from farmland and cloning of a novel mesotrione nitroreductase gene","authors":"Luyao Ruan,&nbsp;Leyun Dong,&nbsp;Jiale Ma,&nbsp;Ye Song,&nbsp;Minglai Wan,&nbsp;Jian He,&nbsp;Jiandong Jiang","doi":"10.1007/s10482-025-02114-8","DOIUrl":"10.1007/s10482-025-02114-8","url":null,"abstract":"<div><p>A Gram positive, aerobic, cream-white, motile, and rod-shaped bacterium, designated as P8^T, was isolated from the soil collected from a farmland in JiangXi Province, China. Strain P8^T efficiently transformed herbicide mesotrione to 2-amino-4-methylsulfonylbenzoic acid (AMBA). Strain P8^T showed the highest 16S rRNA gene sequence similarity with <i>Paenibacillus terreus</i> D33^T (97.4%). Phylogenetic trees based on 16S rRNA gene and genome sequences indicated that strain P8^T formed a distinct clade within the genus <i>Paenibacillus</i>. The major cellular fatty acids were anteiso-C_15:0, C_16:0, anteiso-C_17:0 and iso-C_16:0, the predominant menaquinone was menaquinone-7 (MK-7), the polar lipids profile consisted of phosphatidyl ethanolamine (PE), phosphatidyl monomethyl ethanolamine (PME), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), one unidentified aminolipid (AL), one unidentified aminophospholipid (APL), two unidentified glycolipids (GL1-2), two unidentified phospholipids (PL1-2), and two unidentified polar lipids (L1-2). The DNA G + C content was 49.9%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between P8^T and closely related type strains in genus <i>Paenibacillus</i> were 70.4–93.0% and 13.0–48.3%, respectively. Based on the phenotypic, phylogenetic and genomic results, strain P8^T represents a novel species of the genus <i>Paenibacillus</i>, for which the name <i>Paenibacillus jiagnxiensis</i> sp. nov. is proposed, with strain P8^T (= KCTC 43704^T = MCCC 1K09190^T) as the type strain. Furthermore, a nitroreductase gene (WP355320791), designated as <i>mstnr</i>, that efficiently transformed mesotrione to AMBA was identified in strain P8^T. Mstnr consists of 219 amino acids, and shares low similarities (less than 32%) with reported mesotrione nitroreductases.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144530906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification and characterization of cellulase produced by Bacillus sp. HMM","authors":"Lanqian Huang,&nbsp;Lei Mo,&nbsp;Jiaming Jiang,&nbsp;Yu Huo,&nbsp;Shisong Yu,&nbsp;Jianmei Ou,&nbsp;Siyu Liu,&nbsp;Xinyu Xie,&nbsp;Guyu Yan,&nbsp;Chuzun Liao,&nbsp;Shangxin Wu,&nbsp;Huiqing Zhang,&nbsp;Shiru Huang,&nbsp;Qing Wang,&nbsp;Changhua Shang","doi":"10.1007/s10482-025-02112-w","DOIUrl":"10.1007/s10482-025-02112-w","url":null,"abstract":"<div><p>Cellulose is long-chain glucose polymer, which is linked by β-1,4-glycosidic bond. Its degradation relies on the synergistic action of multi-component cellulase system, including endoglucanase, exoglucanase and β-glucosidase. Cellulase hydrolyzes cellulose to produce glucose through the sequential reactions. While the biodegradation of lignocellulosic wastes has been extensively studied, researches on cellulase production with the mixed agricultural wastes as carbon sources remain limited. To address this gap, this study innovatively employed wheat bran (WB), sugarcane bagasse (SB) and rice hull (RH) to prepare the mixed carbon sources for cellulase production by <i>Bacillus</i> sp. HMM. <i>Bacillus</i> sp. HMM in culture medium with WB showed the highest cellulase activity (3.93 ± 0.13 U/mL) and reducing sugar content (0.97 ± 0.02 mg/mL) at 12 h. Cellulase was purified by (NH₄)₂SO₄ and DEAE-52 column, and investigated by SDS-PAGE. Mw of purified cellulase was between 50 and 70 kDa with purification fold of 23.10. This cellulase had endoglucanase, exoglucanase and β-glucosidase activities, and the wide substrate specificity, which was conducive to the complete degradation of lignocellulose. CMCase activities showed the high resistance under stress conditions such as metal ions, surfactants and inhibitors. CMCase activities still retained over 90% under the treatment of most additives for 4 h, especially DTT greatly promoted CMCase activity, reaching 129.2% of control. <i>Bacillus</i> sp. HMM could efficiently utilize wheat bran to produce cellulase for the conversion of lignocellulosic biomass.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vibrio iocasae sp. nov., a bacterium isolated from the gill homogenate of bivalve lucinids Indoaustriella scarlatoi in intertidal mangrove sediments","authors":"Jia Luo,&nbsp;Huan Zhang,&nbsp;Ziyun Ye,&nbsp;Fei Li,&nbsp;Zhaoshan Zhong,&nbsp;Jiacheng Wang,&nbsp;Minxiao Wang,&nbsp;Chaolun Li","doi":"10.1007/s10482-025-02110-y","DOIUrl":"10.1007/s10482-025-02110-y","url":null,"abstract":"<div><p>A novel <i>Vibrio</i> strain, HN007^T, was isolated from the gill homogenate of the bivalve lucinid <i>Indoaustriella scarlatoi</i> in intertidal mangrove sediments in Huiwen town, Hainan province, PR China. Cells of strain HN007^T were Gram-stain-negative, facultatively anaerobic, motile, rod-shaped, and positive for oxidase and catalase activities. Growth occurred at 16–39 °C, at pH 6.0–11.0 and with 0–7% (w/v) NaCl. Molecular phylogenetic analysis based on 16S rRNA gene sequences affiliated the strain to the members of the genus <i>Vibrio</i>, with the closest species being <i>Vibrio hannami</i> 168GH5-2-16^T (96.9% similarity), <i>Vibrio variabilis</i> R-40492^T (96.1%), <i>Vibrio aestivus</i> M22^T (95.8%), <i>Vibrio maritimus</i> R-40493^T (95.4%), <i>Vibrio mexicanus</i> CAIM 1540^T (95.4%), and <i>Vibrio profundi</i> TP187^T (95.3%). The genome size of strain HN007^T was 5,834,927 bp, with a DNA G + C content of 43.5%. The average nucleotide identity, digital DNA-DNA hybridisation, and average amino acid identity values between strain HN007^T and the most related strains, were all below the thresholds typically used to define genomic species. Further genomic analysis clustered strain HN007^T with the reference strain <i>V. hannami</i> LMG 30079^T within the Albus clade. The major cellular fatty acids of strain HN007^T were summed feature 3 (C_16:1<i>ω</i>6<i>c</i> and/or C_16:1<i>ω</i>7<i>c</i>, 40.9%) and summed feature 8 (C_18:1 <i>ω</i>7<i>c</i> and/or C_18:1 <i>ω</i>6<i>c</i>, 29.4%). The predominant polar lipid was phosphatidylethanolamine, while ubiquinone-8 was the predominant quinone. Based on genomic, chemotaxonomic, and physiological analysis, strain HN007^T represents a novel species within the genus <i>Vibrio</i>, proposed to be named <i>Vibrio iocasae</i> sp. nov. The type strain is HN007^T (= MCCC 1K08504^T = KCTC 92899^T = JCM 35938^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable forest soil management for remediation of heavy metals contamination: integrating technologies and ecosystem health","authors":"Abiodun Yusuff Moshood,&nbsp;Mukhtar Iderawumi Abdulraheem,&nbsp;Linze Li,&nbsp;Yanyan Zhang,&nbsp;Wei Zhang,&nbsp;Kuan Gen,&nbsp;Yiheng Zang,&nbsp;Vijaya Raghavan,&nbsp;Jiandong Hu","doi":"10.1007/s10482-025-02111-x","DOIUrl":"10.1007/s10482-025-02111-x","url":null,"abstract":"<div><p>Due to its persistence and non-biodegradability, heavy metal pollution in forest soils poses an ecological disaster and a severe environmental concern, necessitating sustainable mitigation solutions. Forest soils exhibit physicochemical and biological properties, such as high organic matter content, abundant clay minerals, and diverse microbial interactions, which facilitate key processes such as adsorption, precipitation, and microbial-mediated immobilization of heavy metals. These attributes collectively enhance their capacity for sustainable remediation. Despite extensive research efforts in environmental remediation, there remains a gap in synthesizing the diverse approaches and technologies used for addressing forest soil contamination by heavy metals. Hence, this review aims to bridge this gap by consolidating existing knowledge and highlighting areas that require further investigation by providing an overview of research progress, prospects, and trends in soil remediation of heavy metal contamination. We discuss various remediation methods (e.g., phytoremediation, microbial interactions, and soil amendments), evaluating their effectiveness in reducing heavy metal concentrations and their impact on ecosystem health. A detailed analysis of these methods is presented, including their limitations, applicability, and the need to consider site-specific factors, cost-effectiveness, sustainability, and long-term efficacy. Additionally, we stress the importance of continuous innovation and collaboration among researchers, policymakers, and industry stakeholders to develop multidisciplinary, technology-driven solutions for restoring contaminated soils and safeguarding human and ecosystem health. By integrating multidisciplinary approaches and leveraging emerging technologies, sustainable solutions can be developed to restore contaminated soils and protect human health and ecosystems. Future research should focus on exploring the long-term impacts of forest soil management practices on heavy metal bioavailability and mobility, as well as optimizing existing techniques, exploring novel approaches, assessing ecosystem responses, and integrating forest soil management into broader environmental restoration strategies.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144477739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}