Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology最新文献

{"title":"Undibacterium cyanobacteriorum sp. nov., an auxin-producing bacterium isolated from fresh water during cyanobacterial bloom period","authors":"Ve Van Le,&nbsp;So-Ra Ko,&nbsp;Loan Thi Thanh Nguyen,&nbsp;Jin-Cheol Kim,&nbsp;Yuna Shin,&nbsp;Kyunghyun Kim,&nbsp;Chi-Yong Ahn","doi":"10.1007/s10482-024-01992-8","DOIUrl":"10.1007/s10482-024-01992-8","url":null,"abstract":"<div><p>A novel Gram-negative, white-pigmented, and auxin-producing strain, 20NA77.5^T, was isolated from fresh water during cyanobacterial bloom period. Pairwise comparison of the 16S rRNA gene sequences showed that strain 20NA77.5^T belonged to the genus <i>Undibacterium</i> and exhibited the highest sequence similarity to the type strains of <i>Undibacterium danionis</i> (98.00%), <i>Undibacterium baiyunense</i> (97.93%), <i>Undibacterium macrobrachii</i> (97.92%), and <i>Undibacterium fentianense</i> (97.71%)<i>.</i> The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain 20NA77.5^T and its related type strains were below 79.93 and 23.80%, respectively. The predominant fatty acids (&gt; 10% of the total fatty acids) were C_16:0 and summed feature 3 (C_16:1<i>ω</i>7<i>c</i> and/or C_16:1<i>ω</i>6<i>c</i>). The genomic DNA G + C content of strain 20NA77.5^T was found to be 48.61%. Based on the phylogenetic distinctness, chemotaxonomic features, and phenotypic features, strain 20NA77.5^T is considered to represent a novel species of the genus <i>Undibacterium,</i> for which the name <i>Undibacterium cyanobacteriorum</i> sp. nov is proposed. The type strain is 20NA77.5^T (= KCTC 8005^T = LMG 33136^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141565057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sphaerotilus uruguayifluvii sp. nov., a novel filamentous bacterium isolated from river water","authors":"Eliana V. Machin,&nbsp;Diego M. Roldán,&nbsp;Rodolfo Javier Menes","doi":"10.1007/s10482-024-01996-4","DOIUrl":"10.1007/s10482-024-01996-4","url":null,"abstract":"<div><p>Strain C29^T, a Gram-staining-negative, straight rod occurring singly, in pairs or short chains, was isolated from floating filamentous biomass of the Uruguay River. The strain was catalase and oxidase positive, chemoorganotrophic, strictly aerobic, non-motile, and grew at pH 6.0–9.0, 15–45 °C, and 0–0.5% (w/v) NaCl. Polyhydroxybutyrate was accumulated in nutrient-limited conditions. Phylogenetic analysis based on the 16S rRNA gene revealed that strain C29^T had the highest sequence similarity with <i>Leptothrix discophora</i> SS-1^T (97.82%), <i>Ideonella livida</i> TBM-1^T (97.82%), <i>Vitreoscilla filiformis</i> L1401-2^T (97.52%), <i>Sphaerotilus sulfidivorans</i> D-501^T (97.50%) and <i>Sphaerotilus natans</i> DSM 6575^T (97.46%). Other type strains with validly published names had similarities below 97.46%. Further phylogenomic analysis showed that strain C29^T was affiliated to the family <i>Sphaerotilaceae</i>. Average nucleotide identity (ANI) and in silico DNA–DNA hybridization (dDDH) values with its phylogenetic relatives were lower than 91 and 41%, respectively, revealing that strain C29^T represented a new species. The DNA G + C content of strain C29^T was 70.9%. The annotation of the genome of the novel strain shows it possessed genes for the degradation of aromatic compounds. It also contained genes that encode sigma factors involved in response regulation of stress resistance, which is an important function for adaptation and survival in natural niches. Based on the results of the phylogenetic and phenotypic analyses, we propose that strain C29^T represents a novel species, for which the name <i>Sphaerotilus uruguayifluvii</i> sp. nov. is proposed. The type strain is C29^T (= CCM 9043^T = DSM 113250^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141560274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photobacterium pectinilyticum sp. nov., a novel bacterium isolated from surface seawater of Qingdao offshore","authors":"Yunqi Ding,&nbsp;Pingping Zhang,&nbsp;Yi Liu,&nbsp;Mei Liu,&nbsp;Yaru Wang,&nbsp;Ronghua Liu,&nbsp;Shuxian Yu,&nbsp;Xiao-Hua Zhang,&nbsp;Xiaolei Wang","doi":"10.1007/s10482-024-01979-5","DOIUrl":"10.1007/s10482-024-01979-5","url":null,"abstract":"<div><p>A Gram–staining–negative, facultative aerobic, motile strain, designated strain ZSDE20^T, was isolated from the surface seawater of Qingdao offshore. Phylogenetic analysis of the 16S rRNA gene of strain ZSDE20^T, affiliated it to the genus <i>Photobacterium</i>. It was closely related to <i>Photobacterium lutimaris</i> DF-42^T (98.92% 16S rRNA gene sequence similarity). Growth occurred at 4-28ºC (optimum 28ºC), pH 1.0–7.0 (optimum 7.0) and in the presence of 1–7% (w/v) NaCl (optimum 3%). The dominant fatty acids were summed feature 3 (C16:1 <i>ω</i>7<i>c</i> or/and C16:1 <i>ω</i>6<i>c</i>, 34.23%), summed feature 8 (C18:1 <i>ω</i>7<i>c</i> and C18:1 <i>ω</i>6<i>c</i>, 10.36%) and C16:0 (20.05%). The polar lipids of strain ZSDE20^T comprised phosphatidylethanolamine, phosphatidylcholine, lyso-phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol dimannoside, phosphatidylinositol mannosides and two unknown lipids. The major respiratory quinone was ubiquinone-8 (Q-8). The DNA G + C content of strain ZSDE20^T was 45.6 mol%. Average nucleotide identity (ANI) values between ZSDE20^T and its reference species were lower than the threshold for species delineation (95–96%); in silico DNA-DNA hybridization further showed that strain ZSDE20^T had less than 70% similarity to its relatives. Based on the polyphasic evidences, strain ZSDE20^T is proposed as representing a novel species of the genus <i>Photobacterium</i>, for which the name <i>Photobacterium pectinilyticum</i> sp. nov. is proposed. The type strain is ZSDE20^T (= MCCC 1K06283^T = KCTC 82885^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141560263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pedobacter deserti sp. nov., a novel species isolated from desert soil","authors":"Jie Huang,&nbsp;Shuai Li,&nbsp;Ting-Ting She,&nbsp;Jun Liu,&nbsp;Yi-Jun Mo,&nbsp;Wen-Hui Lian,&nbsp;Dong-Ya Zhang,&nbsp;Lei Dong,&nbsp;Wen-Jun Li","doi":"10.1007/s10482-024-01997-3","DOIUrl":"10.1007/s10482-024-01997-3","url":null,"abstract":"<div><p>An aerobic, Gram-stain-negative bacterium, designated as SYSU D00382^T, was sourced from soil of Gurbantunggut Desert, PR China. The strain was short-rod-shaped, oxidase-positive and catalase-negative, with yellow-colored, convex, round, and smooth colonies on TSA plate. Growth and proliferation occurred at 4–37 °C (optimal: 28–30 °C), pH 5.0–8.0 (optimal: pH 6.0–7.0) and NaCl concentration of 0–2.5% (optimal: 0–0.5%). The 16S rRNA gene based phylogenetic assessment showed that SYSU D00382^T belonged to the genus <i>Pedobacter</i>, and was most closely related to <i>Pedobacter ginsengisoli</i> Gsoil 104^T with similarity of 97.7%. The genomic DNA G+C content of SYSU D00382^T was 46.4%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between SYSU D00382^T and <i>P</i>. <i>ginsengisoli</i> Gsoil 104^T were 75.7% and 17.5%, respectively. The main polar lipid was phosphatidylethanolamine. The major fatty acids (&gt; 5%) were iso-C_15:0, iso-C_17:0 3-OH, summed features 3 and 9. The sole respiratory quinone identified was MK-7. The phylogeny based on 16S rRNA gene and whole-genome sequences revealed that SYSU D00382^T formed a robust lineage with <i>P</i>. <i>ginsengisoli</i> Gsoil 104^T. Based on phenotypic, phylogenetic and genotypic data, a novel specie named <i>Pedobacter deserti</i> sp. nov. is proposed. The type strain is SYSU D00382^T (= CGMCC 1.18627^T = MCCC 1K04972^T = KCTC 82279^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141565056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Secondary metabolite profile of Streptomyces spp. changes when grown with the sub-lethal concentration of silver nanoparticles: possible implication in novel compound discovery","authors":"Merajuddin Khan,&nbsp;Hamad Z. Alkhathlan,&nbsp;Syed Farooq Adil,&nbsp;Mohammed Rafi Shaik,&nbsp;Mohammed Rafiq H. Siddiqui,&nbsp;Mujeeb Khan,&nbsp;Shams Tabrez Khan","doi":"10.1007/s10482-024-01991-9","DOIUrl":"10.1007/s10482-024-01991-9","url":null,"abstract":"<div><p>The decline of new antibiotics and the emergence of multidrug resistance in pathogens necessitates a revisit of strategies used for lead compound discovery. This study proposes to induce the production of bioactive compounds with sub-lethal concentrations of silver nanoparticles (Ag-NPs). A total of Forty-two Actinobacteria isolates from four Saudi soil samples were grown with and without sub-lethal concentration of Ag-NPs (50 µg ml⁻¹). The spent broth grown with Ag-NPs, or without Ag-NPs were screened for antimicrobial activity against four bacteria. Interestingly, out of 42 strains, broths of three strains grown with sub-lethal concentration of Ag-NPs exhibit antimicrobial activity against <i>Staphylococcus aureus</i> and <i>Micrococcus luteus</i>. Among these, two strains S4-4 and S4-21 identified as <i>Streptomyces labedae</i> and <i>Streptomyces tirandamycinicus</i> based on 16S rRNA gene sequence were selected for detailed study. The change in the secondary metabolites profile in the presence of Ag-NPs was evaluated using GC–MS and LC–MS analyses. Butanol extracts of spent broth grown with Ag-NPs exhibit strong antimicrobial activity against <i>M</i>. <i>luteus</i> and <i>S</i>. <i>aureus</i>. While the extracts of the controls with the same concentration of Ag-NPs do not show any activity. GC-analysis revealed a clear change in the secondary metabolite profile when grown with Ag-NPs. Similarly, the LC–MS patterns also differ significantly. Results of this study, strongly suggest that sub-lethal concentrations of Ag-NPs influence the production of secondary metabolites by <i>Streptomyces</i>. Besides, LC–MS results identified possible secondary metabolites, associated with oxidative stress and antimicrobial activities. This strategy can be used to possibly induce cryptic biosynthetic gene clusters for the discovery of new lead compounds.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141535859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene-centered metagenome analysis of Vulcano Island soil (Aeolian archipelago, Italy) reveals diverse microbial key players in methane, hydrogen and sulfur cycles","authors":"Federica Angius,&nbsp;Geert Cremers,&nbsp;Jeroen Frank,&nbsp;Caitlyn Witkowski,&nbsp;Arjan Pol,&nbsp;Theo A. van Alen,&nbsp;Mike S. M. Jetten,&nbsp;Huub J. M. Op den Camp,&nbsp;Tom Berben","doi":"10.1007/s10482-024-01995-5","DOIUrl":"10.1007/s10482-024-01995-5","url":null,"abstract":"<div><p>The Aeolian archipelago is known worldwide for its volcanic activity and hydrothermal emissions, of mainly carbon dioxide and hydrogen sulfide. Hydrogen, methane, and carbon monoxide are minor components of these emissions which together can feed large quantities of bacteria and archaea that do contribute to the removal of these notorious greenhouse gases. Here we analyzed the metagenome of samples taken from the Levante bay on Vulcano Island, Italy. Using a gene-centric approach, the hydrothermal vent community appeared to be dominated by <i>Proteobacteria</i>, and <i>Sulfurimonas</i> was the most abundant genus. Metabolic reconstructions highlight a prominent role of formaldehyde oxidation and the reverse TCA cycle in carbon fixation. [NiFe]-hydrogenases seemed to constitute the preferred strategy to oxidize H₂, indicating that besides H₂S, H₂ could be an essential electron donor in this system. Moreover, the sulfur cycle analysis showed a high abundance and diversity of sulfate reduction genes underpinning the H₂S production. This study covers the diversity and metabolic potential of the microbial soil community in Levante bay and adds to our understanding of the biogeochemistry of volcanic ecosystems.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11219375/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141494154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesobacterium hydrothermale sp. nov., isolated from shallow-sea hydrothermal systems off Kueishantao Island","authors":"Zhiyi Su,&nbsp;Yue Xu,&nbsp;Yuhang Xiao,&nbsp;Beihan Chen,&nbsp;Xuanyun Qiu,&nbsp;Jianing Ye,&nbsp;Kai Tang","doi":"10.1007/s10482-024-01994-6","DOIUrl":"10.1007/s10482-024-01994-6","url":null,"abstract":"<div><p>A Gram-negative, rod-shaped, non-motile, aerobic bacterium, designated as strain TK19101^T, was isolated from the intermediate seawater of yellow vent in the shallow-sea hydrothermal system located near Kueishantao Island. The strain was found to grow at 10–40 °C (optimum, 35 °C), at pH 6.0–8.0 (optimum, 7.0), and in 0–5% (w/v) NaCl (optimum, 1%). Strain TK19101^T was catalase-positive and oxidase-positive. The predominant fatty acids (&gt; 10%) in strain TK19101^T cells were C_16:0, summed feature 8 (C_18:1 <i>ω</i>6<i>c</i> and/or C_18:1 <i>ω</i>7<i>c</i>), and C_18:0. The predominant isoprenoid quinone of strain TK19101^T was ubiquinone-10. The polar lipids of strain TK19101^T comprised phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phospholipid, and unknown polar lipid. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TK19101^T belonged to the genus <i>Mesobacterium</i>. Strain TK19101^T exhibited highest 16S rRNA gene sequence similarity value to <i>Mesobacterium pallidum</i> MCCC M24557^T (97.48%). The estimated average nucleotide identity and digital DNA-DNA hybridization values between strain TK19101^T and the closest related species <i>Mesobacterium pallidum</i> MCCC M24557^T were 74.88% and 20.30%, respectively. The DNA G + C content was 63.49 mol%. On the basis of the analysis of 16S rRNA gene sequences, genotypic and phylogenetic data, strain TK19101^T has a unique phylogenetic status and represents a novel species of genus <i>Mesobacterium</i>, for which the name <i>Mesobacterium hydrothermale</i> sp. nov. is proposed. The type strain is TK19101^T (= MCCC 1K08936^T = KCTC 8354^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141494155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol potential of plant growth-promoting rhizobacteria against plant disease and insect pest","authors":"Qinhao Jian,&nbsp;Tongrui Zhang,&nbsp;Yingying Wang,&nbsp;Li Guan,&nbsp;Linlin Li,&nbsp;Longna Wu,&nbsp;Shiyan Chen,&nbsp;Yumei He,&nbsp;Hong Huang,&nbsp;Shugang Tian,&nbsp;Hu Tang,&nbsp;Litang Lu","doi":"10.1007/s10482-024-01975-9","DOIUrl":"10.1007/s10482-024-01975-9","url":null,"abstract":"<div><p>Biological control is a promising approach to enhance pathogen and pest control to ensure high productivity in cash crop production. Therefore, PGPR biofertilizers are very suitable for application in the cultivation of tea plants (<i>Camellia sinensis</i>) and tobacco, but it is rarely reported so far. In this study, production of a consortium of three strains of PGPR were applied to tobacco and tea plants. The results demonstrated that plants treated with PGPR exhibited enhanced resistance against the bacterial pathogen <i>Pseudomonas syringae</i> (PstDC3000). The significant effect in improving the plant's ability to resist pathogen invasion was verified through measurements of oxygen activity, bacterial colony counts, and expression levels of resistance-related genes (<i>NPR1</i>, <i>PR1</i>, <i>JAZ1</i>, <i>POD</i> etc.). Moreover, the application of PGPR in the tea plantation showed significantly reduced population occurrences of tea green leafhoppers (<i>Empoasca onukii Matsuda</i>), tea thrips (<i>Thysanoptera:Thripidae</i>), <i>Aleurocanthus spiniferus</i> (<i>Quaintanca</i>) and alleviated anthracnose disease in tea seedlings. Therefore, PGPR biofertilizers may serve as a viable biological control method to improve tobacco and tea plant yield and quality. Our findings revealed part of the mechanism by which PGPR helped improve plant biostresses resistance, enabling better application in agricultural production.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141472221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Denitrificimonas halotolerans sp. nov., a novel species isolated from UASB sludge treating landfill leachate","authors":"Shanhui Wang,&nbsp;Chu Yuan,&nbsp;Chengbin Xu,&nbsp;Ditian Li,&nbsp;Han Zhang,&nbsp;Jing Wang,&nbsp;Xin Wang,&nbsp;Yan Li,&nbsp;Dian Jiao,&nbsp;Siliang Yuan,&nbsp;Han Chen,&nbsp;Dongru Qiu","doi":"10.1007/s10482-024-01987-5","DOIUrl":"10.1007/s10482-024-01987-5","url":null,"abstract":"<div><p>A Gram-stain-negative, facultative anaerobe, rod-shaped strain JX-1^T was isolated from UASB sludge treating landfill leachate in Wuhan, China. The isolate is capable of growing under conditions of pH 6.0–11.0 (optimum, pH 7.0–8.0), temperature 4–42 ℃ (optimum, 20–30 ℃), 0–8.0% (w/v) NaCl (optimum, 5.0%), and ammonia nitrogen concentration of 200–5000 mg/L (optimum, 500 mg/L) on LB plates. The microorganism can utilize malic acid, D-galactose, L-rhamnose, inosine, and L-glutamic acid as carbon sources, but does not reduce nitrates and nitrites. The major fatty acids are C_18:1ω7c/C_18:1ω6c, iso-C_15:0, and anteiso-C_15:0. The respiratory quinones are Q9 (91.92%) and Q8 (8.08%). Polar lipids include aminolipid, aminophospholipid, diphosphatidylglycerol, glycolipid, phosphatidylethanolamine, phosphatidylglycerol, and phospholipid. Compared with other strains, strain JX-1^T and <i>Denitrificimonas caeni</i> HY-14^T have the highest values in terms of 16S rRNA gene sequence similarity (96.79%), average nucleotide identity (ANI; 76.06%), and average amino acid identity (AAI; 78.89%). Its digital DNA-DNA hybridization (dDDH) result is 20.3%. The genome of strain JX-1^T, with a size of 2.78 Mb and 46.12 mol% G + C content, lacks genes for denitrification and dissimilatory nitrate reduction to ammonium (DNRA), but contains genes for ectoine synthesis as a secondary metabolite. The results of this polyphasic study allow genotypic and phenotypic differentiation of the analysed strain from the closest related species and confirm that the strain represents a novel species within the genus <i>Denitrificimonas</i>, for which the name <i>Denitrificimonas halotolerans</i> sp. nov. is proposed with JX-1^T (= MCCC 1K08958^T = KCTC 8395^T) as the type strain.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141441035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Genome-based classification of genera Halosegnis and Salella, and description of four novel halophilic archaea isolated from a tidal flat","authors":"Yao Hu,&nbsp;Xue Ma,&nbsp;Shun Tan,&nbsp;Xin-Xin Li,&nbsp;Mu Cheng,&nbsp;Jing Hou,&nbsp;Heng-Lin Cui","doi":"10.1007/s10482-024-01983-9","DOIUrl":"10.1007/s10482-024-01983-9","url":null,"abstract":"","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141332433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}