Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology最新文献

{"title":"Genomic analysis of Massilia antarctica subsp. insulae subsp. nov., a violacein- and aminochelin-producing bacterium isolated from Horseshoe Island, Antarctica","authors":"Sibel Melisa Sahin,&nbsp;Izzet Burcin Saticioglu,&nbsp;Muhammed Duman,&nbsp;Sabriye Sel,&nbsp;Hilal Ay","doi":"10.1007/s10482-025-02107-7","DOIUrl":"10.1007/s10482-025-02107-7","url":null,"abstract":"<div><p>Microorganisms isolated from polar regions are recognized for their considerable capability to encode unique proteins and metabolic pathways with importance for biotechnological applications. Specifically, microorganisms capable of generating pigments possessing antioxidant and antimicrobial properties as a strategy for survival amidst challenges like high ultraviolet radiation, low temperature, oxidative stress and microbial competition are noteworthy. In this research, we discovered a new subspecies of the species <i>Massilia antarctica</i>, designated as H27-R4^T, which was isolated from a soil sample collected at Horseshoe Island in Antarctica. Strain H27-R4^T is a purple-pigmented, rod-shaped, Gram-negative, and aerobic bacterium. Pairwise comparison of the 16S rRNA genes showed that the strain shares 100% identity with the type strain of <i>Massilia antarctica</i> P8398^T and <i>Massilia frigida</i> CCM 8695^T, while the highest average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of 97.1% and 73.7%, respectively, were observed between strain H27-R4^T and <i>M. antarctica</i> P8398^T. The whole-genome analysis revealed a metabolic potential in terms of secondary metabolism, resistance to antimicrobials and heavy metals, adaptation to cold environments, stress response mechanisms and metabolism of aromatic compounds. In addition, the strain exhibited pathways for violacein and aminochelin biosynthesis. Phylogenomic analyses confirmed that strain H27-R4^T represents a novel subspecies for which the name <i>Massilia antarctica</i> subsp. <i>insulae</i> subsp. nov. is proposed.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144477738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological interactions and soil properties shape bacterial and fungal communities during restoration of Pinus thunbergii shelter in saline soils","authors":"Zhugui Wen,&nbsp;Xinyu Liu,&nbsp;Wei Zhou,&nbsp;Xinke Liu,&nbsp;Zhaohan Fan,&nbsp;Yuxuan Wang,&nbsp;Boping Tang,&nbsp;Baoming Ge","doi":"10.1007/s10482-025-02108-6","DOIUrl":"10.1007/s10482-025-02108-6","url":null,"abstract":"<div><p>As a practical management approach to combat global warming, reforestation may affect microbial communities. However, the effects of <i>Pinus thunbergii</i> forest restoration on microbial colonisation and community structures in saline areas are poorly documented. Herein, microbial communities were investigated in two different pine stand age forests, in addition to bare sites, through Illumina MiSeq high-throughput sequencing technology based on bacterial 16S rRNA and fungal ITS rRNA, and predicted using the PICRUSt2 and FUNGuild databases. The restoration of <i>P. thunbergii</i> shelter in saline areas caused significant changes in soil properties and microbial community characteristics. NMDS analysis exhibited significant differences in fungal and bacterial composition, as confirmed by the Adonis test (<i>p</i> = 0.04 and <i>p</i> &lt; 0.01). Microbial communities were significantly affected by soil properties, with fungal and bacterial diversity being positively affected by soil’s rapidly available-K (R² = 0.15 and 0.22) and hydrolysable-N (R² = 0.16 and 0.11). Of all guilds, endophytes and mycorrhizal fungi had a marked effect on specific bacterial compositions. The topologies of microbial networks showed that microbial network complexity was lower in low-growth forests, while stable below-ground microbial community structures could form under long-term <i>P. thunbergii</i> vegetation. During the restoration process, soil fungi were less susceptible to the vegetation successional stage, while soil bacteria were more sensitive. Over time, the diversity of bacteria and fungi was mainly driven by soil characteristics as well as the restoration of <i>P. thunbergii</i>. Further, forest restoration and mycorrhizal fungi could shape specific bacterial community compositions in saline soils.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-vitro assessment of probiotic potential of bacteria isolated from chicken gastrointestinal tract","authors":"Fatima Shahbaz,&nbsp;Fatima Muccee,&nbsp;Afreen Fatima Mirza,&nbsp;Muhammad Abdullah Tanveer,&nbsp;Amal H. I. Al Haddad,&nbsp;Nadia Hussain","doi":"10.1007/s10482-025-02104-w","DOIUrl":"10.1007/s10482-025-02104-w","url":null,"abstract":"<div><p>Broilers farming has been suffering from multiple challenges, including extensive application of antibiotics. Probiotics-supplemented feed might serve as an efficient alternative to these growth stimulants. We designed the present study to isolate bacteria from chickens’ gut and analyze their probiotic characteristics. For isolation, the serial dilution method and De Man–Rogosa–Sharpe agar (MRS) medium were used. Isolates were characterized via biochemical and ribotyping analysis, growth study, antimicrobial and antibiotic sensitivity profiling, acid and bile salt tolerance, intestinal adhesion and cholesterol degradation assays. Four isolates were identified as <i>Enterococcus durans</i> strain PUPro5, <i>Lactobacillus acidophilus</i> strain PUPro6, <i>Enterococcus durans</i> strain PUPro7 and <i>Ligilactobacillus salivarius</i> strain PUPro8. The highest growth rate was observed in <i>E. durans</i> strain PUPro7 with OD₆₀₀ = 1.929 ± 0.001 and start of log phase after 3 h. All isolates showed maximum intestinal cell adhesion at 90 min, ranging between 423 and 588.67 CFUs/ml. All isolates demonstrated maximum tolerance potential at 2% concentration of NaCl with <i>L. salivarius</i> strain PUPro8 being the most efficient among all isolates. The highest resistance to bile salts was observed in <i>L. acidophilus</i> strain PUPro6 with an optimum OD₆₀₀ of 1.748 at log phase. All bacteria demonstrated antimicrobial resistance against <i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i>, <i>Bacillus subtilis</i> and <i>B. proteus</i>. Maximum tolerance was observed in <i>L. acidophilus</i> strain PUPro6 (OD₆₀₀ = 1.344 ± 0.017) against <i>P. aeruginosa</i>, <i>L. salivarius</i> strain PUPro8 (OD₆₀₀ = 1.304 ± 0.003) against <i>B. subtilis</i>, <i>E. durans</i> strain PUPro5 (OD₆₀₀ = 1.294 ± 0.066) against <i>S. aureus</i> and <i>E. durans</i> strain PUPro7 (OD₆₀₀ = 1.216 ± 0.002) against <i>B. proteus</i>. All isolates were sensitive against all the antibiotics, with maximum zone of inhibition against velosef and cefadroxil in the <i>E. durans</i> strain PUPro5. Cholesterol degradation efficiency of isolates ranged between 195.2 and 204.6 mg/dl. Bacteria characterized in the present study exhibit probiotic potential and can be recommended for testing their effect on chickens’ growth and meat quality through broiler diet supplementation.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144334385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Siderophores produced by marine microorganisms","authors":"Lei Chen,&nbsp;Zhuo-Yue Li,&nbsp;Guang-Yu Wang","doi":"10.1007/s10482-025-02098-5","DOIUrl":"10.1007/s10482-025-02098-5","url":null,"abstract":"<div><p>Siderophores are iron-chelating, low-molecular-weight compounds secreted by microorganisms in iron-deficient environments that enhance iron uptake. When compared to the siderophores produced by terrestrial microorganisms, marine siderophores have been shown to have diverse structures and physiological functions. Fifty-seven strains of marine microorganisms have been identified as siderophore producers so far. Among them, 54 bacterial strains belong to 23 genera of 19 families in five phyla, and three fungal strains belong to three genera of three families in two phyla. The most common source of siderophores is seawater, which has a wide depth distribution. A total of 105 siderophores from marine microorganisms had been reported by 2024. These siderophores are mainly divided into five types depending on their functional groups and hydrophobicity: hydroxamates, catecholates, carboxylates, mixed, and other types. The most common marine siderophore is hydroxamate. The amphiphilicity of marine microbial siderophores distinguishes them from those produced by terrestrial microorganisms. This review discusses the recent findings in marine siderophore biology and highlights the potential of siderophores in medicine, fish disease control, bioremediation, and biosensors.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrons: the hidden architects of bacterial adaptation, evolution, and the challenges of antimicrobial resistance","authors":"Harjeet Singh,&nbsp;Shivani Pandya,&nbsp;Srushti Jasani,&nbsp;Mitesh Patel,&nbsp;Tanvir Kaur,&nbsp;Sarvesh Rustagi,&nbsp;Sheikh Shreaz,&nbsp;Ajar Nath Yadav","doi":"10.1007/s10482-025-02103-x","DOIUrl":"10.1007/s10482-025-02103-x","url":null,"abstract":"<div><p>Integrons, a diverse group of genetic elements, have emerged as key players in bacterial adaptation and evolution. These elements, commonly found in both environmental as well as clinical settings, facilitate the acquisition, exchange, and expression of integron cassettes, allowing bacteria to rapidly adapt to changing environments and acquire antibiotic resistance. This review provides an in-depth exploration of the various classes of clinical integrons, including class 1, 2, and 3, highlighting their origins, distribution, and associated mobile elements. We delve into the astonishing success of “class 1 integrons”, emphasizing their ability to recognize diverse attachment sites known as “<i>attC</i> sites” and getting integrated within many different integron cassettes from diverse sources. Class 1 integrons are able to propagate widely among bacterial hosts due to their lack of host specificity, interaction with transposons, and broad host range plasmids. Moreover, we discuss the substantial impact of class 1 integrons in antimicrobial resistance, as they accumulate an array of resistance genes through strong positive selection. Additionally, we address the challenging issue regarding the evolution and function of integrons and integron cassettes, including the role of promoters, origins of integron cassettes, and the abundance of unknown proteins encoded within them. The future prospects of integron research are also explored, highlighting the need to understand cassette expression patterns, assess the contribution of chromosomal/superintegron arrays to host fitness, unravel the mechanisms of cassette generation, and investigate the connection between the SOS induction and horizontal gene transfer. Overall, this review underlines the significance of integrons as hidden architects driving bacterial adaptation and evolution, providing valuable insights into their ecological and evolutionary dynamics, and shaping the future direction of research in this field.</p><h3>Graphical abstract</h3><p><b>A</b> Antibiotics-mediated cascade activation; <b>B</b> Reshuffling of integron cassettes in sedentary chromosomal integrons and mobile integron cassettes transfer between bacteria for antimicrobial resistance</p>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of microbiota in fish spoilage: biochemical mechanisms and innovative preservation strategies","authors":"Yongzhou Chi,&nbsp;Meilin Luo,&nbsp;Chengcheng Ding","doi":"10.1007/s10482-025-02101-z","DOIUrl":"10.1007/s10482-025-02101-z","url":null,"abstract":"<div><p>Fish spoilage is a microbially-mediated biochemical process resulting in quality deterioration, economic losses, and food safety risks. Studies have indicated that spoilage microbiota are phylogenetically diverse, with Gram-negative bacteria (<i>Pseudomonas</i>, <i>Shewanella</i>, <i>Photobacterium</i>) representing primary spoilage organisms, and Gram-positive bacteria (<i>Lactobacillus</i>, <i>Brochothrix</i>) causing spoilage only under specific conditions. Microorganisms cause spoilage through the utilization of three main metabolic processes: (i) proteolytic degradation of muscle proteins, (ii) lipolytic breakdown of triglycerides, and (iii) production of volatile bioactive organic compounds and biogenic amines. By combining high-throughput sequencing with metabolomics, researchers have been uncovering strain-specific metabolic networks and how they are influenced by environmental factors such as temperature, pH, and packaging. This review systematically examines: (1) patterns of taxonomic succession in spoilage microbiota, (2) enzymatic and biochemical pathways involved in spoilage, and (3) innovative preservation strategies targeting spoilage consortia. Emerging technologies, such as bacteriocin-mediated biopreservation, phage therapy, and modified atmosphere packaging, show considerable promise in inhibiting spoilage organisms while maintaining the sensory qualities of the fish. Microbiome-directed interventions combined with predictive modeling and precision storage systems also represent a novel approach to fish preservation. There is a critical need to integrate traditional microbiology with the use of multi-omic technologies for the development of sustainable, microbiota-based preservation strategies that address global seafood security challenges.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144250594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of endophytic bacteria in enhancing plant growth and health for sustainable agriculture","authors":"Tairine Graziella Ercole,&nbsp;Daniela Romani Bonotto,&nbsp;Mariangela Hungria,&nbsp;Vanessa Merlo Kava,&nbsp;Lygia Vitoria Galli","doi":"10.1007/s10482-025-02100-0","DOIUrl":"10.1007/s10482-025-02100-0","url":null,"abstract":"<div><p>Given the impact of abiotic and biotic factors on agriculture, it is crucial to adopt sustainable and environmentally friendly practices to enhance yields. One approach that is gaining prominence is the utilization of plant-growth-promoting bacteria (PGPB) in mainstream agriculture. Interactions between plants and microbes in the rhizosphere play a vital role in plant health, productivity, and soil fertility. PGPB, particularly endophytes that establish close associations with plants, have shown success in promoting plant growth and protecting plants against diseases and abiotic stresses through various mechanisms, including biological nitrogen fixation, phosphate solubilization, siderophore production, phytohormone production, ACC-deaminase activity, induced systemic resistance, quorum sensing, hydrogen cyanide synthesis, hydrolytic enzyme and colonization niche specialization. While there is no one-size-fits-all strategy for promoting the growth of every plant under every condition, certain strategies discussed in this review already demonstrate significant promise. This review provides an overview of the current understanding of the fundamental mechanisms employed by PGPB, and emphasizes innovative methods for identifying and developing promising strains to serve as bio-inputs. Likewise, the aim of this review is to present a summary of the crucial role that soil–plant-microorganisms interactions play in the development of efficient bioinoculants, as well as to explore biotechnological advances. Bacteria from genera such as <i>Azospirillum, Bacillus, Bradyrhizobium,</i> and <i>Pseudomonas</i> have already been widely studied and applied for these purposes. However, further research is needed to improve strain performance and enhance the potential of multifunctional microorganisms. Deepening the genetic and biochemical understanding of these mechanisms and expanding their applications is essential to foster safe and sustainable agronomic practices.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144250593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoeradication of exopolysaccharide-producing Leuconostoc mesenteroides as a harmful microorganism in the sugarcane processing industry","authors":"Esmaeil Darabpour,&nbsp;Mahya Mohammadi Zeynallou,&nbsp;Mohammad Roayaei Ardakani","doi":"10.1007/s10482-025-02099-4","DOIUrl":"10.1007/s10482-025-02099-4","url":null,"abstract":"<div><p>The production of exopolysaccharides (EPS) by <i>Leuconostoc mesenteroides</i> causes some serious problems in the cane sugar refining process. So, it is necessary to eliminate <i>L. mesenteroides</i> as an undesirable microorganism in the cane sugar refinery. Photodynamic inactivation (PDI) is a novel and attractive strategy to eradicate undesirable microorganisms. In this study, we aimed to evaluate PDI of <i>L. mesenteroides</i> using methylene blue (MB). Suspensions of <i>L. mesenteroides</i> PTCC 1591 was treated by different concentration of methylene blue (6.25–100 µM) and subsequently subjected to laser light (650 nm). Effect of sub-lethal photodynamic inactivation on the production of EPS by <i>L. mesenteroides</i> was also assessed. Meanwhile, the effects of PDI (at lethal and sub-lethal levels) on <i>L. mesenteroides</i> cells were visualized using scanning electron microscopy (SEM). PDI mediated by MB (50 and 100 µM) caused a significant reduction in the number of viable <i>L. mesenteroides</i> cells (&gt; 3 log₁₀ CFU reduction). <i>L. mesenteroides</i> exposed to sub-lethal photodynamic inactivation produced significantly less EPS than untreated cells. Scanning electron microscopy analysis clearly confirmed the potent ability of PDI to kill <i>L. mesenteroides</i> cells. In conclusion, PDI mediated by methylene blue offer a new modality for fast and efficient elimination of <i>L. mesenteroides</i> cells, indicating its potential use in the sugarcane industry.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of climate, weather, seasonal transitions, and diurnal rhythms on gut microbiota and immune homeostasis","authors":"B. Aswinanand,&nbsp;B. Haridevamuthu,&nbsp;Ajay Guru,&nbsp;Jesu Arockiaraj","doi":"10.1007/s10482-025-02097-6","DOIUrl":"10.1007/s10482-025-02097-6","url":null,"abstract":"<div><p>The influence of meteorological environmental factors such as climate change, weather alterations, seasonal transitions, and diurnal cycles on human health is becoming increasingly evident, particularly through their modulation of the gut microbiome and immune homeostasis. The human gut microbiota maintains immune homeostasis and regulates various physiological and metabolic functions by producing metabolites like short-chain fatty acids (SCFAs), bile acids (BAs), indole derivatives (IDs), choline metabolites (CMs), and bacterial vitamins (BVs). Climate change disrupts this gut microbiome's impact through the gut-liver axis, which has been linked to the occurrence of fatty liver diseases. Temperature fluctuations, such as cold exposure, impair the gut microbiota's ability to respond to adaptive thermogenesis by disrupting the <i>Firmicutes</i> to <i>Bacteroides</i> ratio, affecting insulin sensitivity. In contrast, high temperatures support the increase in <i>Proteobacterial</i> populations, leading to greater gut permeability, allowing pathogens to trigger inflammation, oxidative stress, and immune dysregulation. In summer, an increase in <i>Firmicutes</i> contributes to weight gain and obesity, and in contrast, in winter, a rise in <i>Bacteroides</i> exacerbates conditions like rheumatoid arthritis (RA). High humidity also affects the <i>Bacteroides</i>/<i>Firmicutes</i> ratio by increasing and contributing to <i>Bacteroides</i>-driven inflammation. Conversely, lower humidity levels impair the mucosal defense mechanisms, increasing infection susceptibility and gut barrier dysfunction. Alterations in diurnal cycles are also linked to conditions like type 2 diabetes (T2D) and inflammatory bowel disease (IBD), by affecting the gut microbiome. Overall, the review emphasizes the crucial role of these factors in maintaining immune homeostasis through gut microbiome composition, highlighting the importance of understanding these influences for targeted interventions that restore gut health, particularly in relation to meteorologically driven environmental factors.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overview of the Q fever vaccine development: current status and future prospects","authors":"Samira Karimaei,&nbsp;Safoura Moradkasani,&nbsp;Saber Esmaeili","doi":"10.1007/s10482-025-02094-9","DOIUrl":"10.1007/s10482-025-02094-9","url":null,"abstract":"<div><p><i>Coxiella burnetii</i>, the causative agent of Q fever, is responsible for a globally significant zoonotic disease, characterized by flu-like symptoms. The primary reservoirs of <i>C. burnetii</i> are ruminant livestock, particularly goats, sheep, and cattle, which shed the bacterium through birth products, such as the placenta, amniotic fluid, and other secretions. Human infections typically occur via the inhalation of contaminated aerosols during direct or indirect contact with infected animals or their birthing materials. Consequently, individuals living in or working near livestock environments are at elevated risk, making Q fever both a location- and occupation-related disease. Owing to its remarkable environmental resilience and extremely low infectious dose, <i>C. burnetii</i> is classified as a Category B bioterrorism agent by the U.S. Centers for Disease Control and Prevention (CDC). These characteristics significantly complicate efforts to eradicate the bacterium and position vaccination as a key strategy for preventing human transmission. Although whole-cell vaccines (WCVs) are currently licensed for use in Australia, their widespread implementation has been hindered by their strong reactogenic responses in individuals with prior exposure to <i>C. burnetii</i>. This review provides an overview of past and current efforts to develop non-reactogenic <i>C. burnetii</i> vaccines and discusses possible approaches to enhance the efficiency and safety of these vaccines.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}