Parapedobacter flavus sp. nov., isolated from padding of fermentation bed

Abstract

A Gram-stain-negative, aerobic, rod-shaped bacterium strain, designated as 4-7-4^T, was isolated from the padding of a fermentation bed. Phylogenetic analyses based on 16S rRNA gene indicated that it belonged to the genus Parapedobacter and exhibited the closest relationship to P. soli KCTC 12984^T, with a similarity of 96.03%. The average nucleotide identity and digital DNA–DNA hybridization between strain 4-7-4^T and P. soli KCTC 12984^T were 78.6% and 21.6% respectively, both of which were below the thresholds for delineating species, supporting it being a novel species of Parapedobacter. The major polar lipids included phosphatidylethanolamine, unidentified aminolipid and four unidentified lipids. The predominant respiratory quinone was MK-7. The major cellular fatty acids were summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c) and iso-C_15:0. The genome of strain 4-7-4^T was 4890729 bp, and the genomic DNA G + C content was 49.8%. In summary, strain 4-7-4^T was identified as a novel species of Parapedobacter, for which the name Parapedobacter flavus sp. nov. was proposed. The type strain was 4-7-4^T (=CGMCC1.10938^T = KCTC 92351^T).