Development Growth & Differentiation最新文献_第3页

Establishment and characterization of mouse lines useful for endogenous protein degradation via an improved auxin-inducible degron system (AID2) 通过改良的辅助素诱导降解子系统（AID2），建立有助于内源性蛋白质降解的小鼠品系并确定其特征。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2024-09-21 DOI: 10.1111/dgd.12942

Hatsune Makino-Itou, Noriko Yamatani, Akemi Okubo, Makoto Kiso, Rieko Ajima, Masato T. Kanemaki, Yumiko Saga

{"title":"Establishment and characterization of mouse lines useful for endogenous protein degradation via an improved auxin-inducible degron system (AID2)","authors":"Hatsune Makino-Itou, Noriko Yamatani, Akemi Okubo, Makoto Kiso, Rieko Ajima, Masato T. Kanemaki, Yumiko Saga","doi":"10.1111/dgd.12942","DOIUrl":"10.1111/dgd.12942","url":null,"abstract":"The development of new technologies opens new avenues in the research field. Gene knockout is a key method for analyzing gene function in mice. Currently, conditional gene knockout strategies are employed to examine temporal and spatial gene function. However, phenotypes are sometimes not observed because of the time required for depletion due to the long half-life of the target proteins. Protein knockdown using an improved auxin-inducible degron system, AID2, overcomes such difficulties owing to rapid and efficient target depletion. We observed depletion of AID-tagged proteins within a few to several hours by a simple intraperitoneal injection of the auxin analog, 5-Ph-IAA, which is much shorter than the time required for target depletion using conditional gene knockout. Importantly, the loss of protein is reversible, making protein knockdown useful to measure the effects of transient loss of protein function. Here, we also established several mouse lines useful for AID2-medicated protein knockdown, which include knock-in mouse lines in the ROSA26 locus; one expresses TIR1(F74G), and the other is the reporter expressing AID-mCherry. We also established a germ-cell-specific TIR1 line and confirmed the protein knockdown specificity. In addition, we introduced an AID tag to an endogenous protein, DCP2 via the CAS9-mediated gene editing method. We confirmed that the protein was effectively eliminated by TIR1(F74G), which resulted in the similar phenotype observed in knockout mouse within 20 h.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 7","pages":"384-393"},"PeriodicalIF":1.7,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12942","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142300049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chicken embryo cultures in the dorsal-upward orientation for the manipulation of epiblasts 鸡胚背向上方培养用于操作上胚层

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2024-09-17 DOI: 10.1111/dgd.12943

Kaho Konya, Yusaku Watanabe, Akihito Kawamura, Kae Nakamura, Hideaki Iida, Koya Yoshihi, Hisato Kondoh

{"title":"Chicken embryo cultures in the dorsal-upward orientation for the manipulation of epiblasts","authors":"Kaho Konya, Yusaku Watanabe, Akihito Kawamura, Kae Nakamura, Hideaki Iida, Koya Yoshihi, Hisato Kondoh","doi":"10.1111/dgd.12943","DOIUrl":"10.1111/dgd.12943","url":null,"abstract":"Chicken embryos have many advantages in the study of amniote embryonic development. In particular, culture techniques developed for early-stage embryos have promoted the advancement of modern developmental studies using chicken embryos. However, the standard technique involves placing chicken embryos in the ventral-upward (ventral-up) orientation, limiting manipulation of the epiblast at the dorsal surface, which is the primary source of ectodermal and mesodermal tissues. To circumvent this limitation, we developed chicken embryo cultures in the dorsal-up orientation and exploited this technique to address diverse issues. In this article, we first review the history of chicken embryo culture techniques to evaluate the advantages and limitations of the current standard technique. Then, the dorsal-up technique is discussed. These technological discussions are followed by three different examples of experimental analyses using dorsal-up cultures to illustrate their advantages: (1) EdU labeling of epiblast cells to assess potential variation in the cell proliferation rate; (2) migration behaviors of N1 enhancer-active epiblast cells revealed by tracking cells with focal fluorescent dye labeling in dorsal-up embryo culture; and (3) neural crest development of mouse neural stem cells in chicken embryos.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 8","pages":"426-434"},"PeriodicalIF":1.7,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12943","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142257501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meeting report of the 57th Annual Meeting of the Japanese Society for Developmental Biologists 日本发育生物学家学会第 57 届年会会议报告

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2024-09-10 DOI: 10.1111/dgd.12941

Yuzuka Takeuchi

引用次数: 0

Development of specialized devices for microbial experimental evolution 开发微生物实验进化专用设备。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2024-08-26 DOI: 10.1111/dgd.12940

Atsushi Shibai, Chikara Furusawa

引用次数: 0

The self-in-the-world map emerged in the primate brain as a basis for Homo sapiens abilities 自我世界地图出现在灵长类大脑中，是智人能力的基础。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2024-08-08 DOI: 10.1111/dgd.12939

Rafael Bretas, Banty Tia, Atsushi Iriki

{"title":"The self-in-the-world map emerged in the primate brain as a basis for Homo sapiens abilities","authors":"Rafael Bretas, Banty Tia, Atsushi Iriki","doi":"10.1111/dgd.12939","DOIUrl":"10.1111/dgd.12939","url":null,"abstract":"The brain in the genus Homo expanded rapidly during evolution, accelerated by a reciprocated interaction between neural, cognitive, and ecological niches (triadic niche construction, or TNC). This biologically costly expansion incubated latent cognitive capabilities that, with a quick and inexpensive rewiring of brain areas in a second phase of TNC, provided the basis for Homo sapiens specific abilities. The neural demands for perception of the human body in interaction with tools and the environment required highly integrated sensorimotor domains, inducing the parietal lobe expansion seen in humans. These newly expanded brain areas allowed connecting the sensations felt in the body to the actions in the world through the cognitive function of “projection”. In this opinion article, we suggest that as a relationship of equivalence between body parts, tools and their external effects was established, mental mechanisms of self-objectification might have emerged as described previously, grounding notions of spatial organization, idealized objects, and their transformations, as well as socio-emotional states in the sensing agent through a self-in-the-world map. Therefore, human intelligence and its features such as symbolic thought, language, mentalizing, and complex technical and social behaviors could have stemmed from the explicit awareness of the causal relationship between the self and intentional modifications to the environment.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 6","pages":"342-348"},"PeriodicalIF":1.7,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12939","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141903468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endothelial cell transitions in zebrafish vascular development 斑马鱼血管发育过程中的内皮细胞转换

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2024-07-27 DOI: 10.1111/dgd.12938

Li-Kun Phng, Benjamin M. Hogan

引用次数: 0

Zebrafish trpm7 mutants show reduced motility in free movement 斑马鱼 trpm7 突变体在自由运动时运动能力下降。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2024-07-06 DOI: 10.1111/dgd.12937

Kenta Watai, Kenichiro Sadamitsu, Seiji Wada, Makoto Kashima, Hiromi Hirata

{"title":"Zebrafish trpm7 mutants show reduced motility in free movement","authors":"Kenta Watai, Kenichiro Sadamitsu, Seiji Wada, Makoto Kashima, Hiromi Hirata","doi":"10.1111/dgd.12937","DOIUrl":"10.1111/dgd.12937","url":null,"abstract":"Parkinson's disease is a neurological disorder characterized by reduced motility, depression and dementia. Guamanian parkinsonism dementia with amyotrophic sclerosis is a local case of Parkinson's disease reported in the Western Pacific Islands of Guam and Rota as well as in the Kii Peninsula of Japan. A previous genetic study has suggested that Guamanian parkinsonism is attributable to a variant of the TRPM7 gene, which encodes for melastatin-related transient receptor potential (TRP) ion channels. But the link between parkinsonism and the TRPM7 gene remains elusive. Previous studies have addressed that trpm7-deficient zebrafish embryos showed defects in pigmentation and touch-evoked motor response. In this study, we identified a new viable allele of trpm7 mutant causing an I756N amino acid substitution in the first transmembrane domain. Behavioral analyses revealed that trpm7 mutants showed compromised motility with their movement distance shorter than wild-type larvae. The velocity of the movement was significantly reduced in trpm7 mutants than in wild-type larvae. Along with a previous finding of reduced dopaminergic neurons in zebrafish trpm7 mutants, reduced motility of trpm7 mutants can suggest another similarity between trpm7 phenotypes and Parkinson's disease symptoms.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 6","pages":"349-356"},"PeriodicalIF":1.7,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141545503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential cellular stiffness across tissues that contribute to Xenopus neural tube closure 造成爪蟾神经管闭合的不同组织细胞硬度的差异。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2024-06-26 DOI: 10.1111/dgd.12936

Makoto Suzuki, Naoko Yasue, Naoto Ueno

{"title":"Differential cellular stiffness across tissues that contribute to Xenopus neural tube closure","authors":"Makoto Suzuki, Naoko Yasue, Naoto Ueno","doi":"10.1111/dgd.12936","DOIUrl":"10.1111/dgd.12936","url":null,"abstract":"During the formation of the neural tube, the primordium of the vertebrate central nervous system, the actomyosin activity of cells in different regions drives neural plate bending. However, how the stiffness of the neural plate and surrounding tissues is regulated and mechanically influences neural plate bending has not been elucidated. Here, we used atomic force microscopy to reveal the relationship between the stiffness of the neural plate and the mesoderm during Xenopus neural tube formation. Measurements with intact embryos revealed that the stiffness of the neural plate was consistently higher compared with the non-neural ectoderm and that it increased in an actomyosin activity-dependent manner during neural plate bending. Interestingly, measurements of isolated tissue explants also revealed that the relationship between the stiffness of the apical and basal sides of the neural plate was reversed during bending and that the stiffness of the mesoderm was lower than that of the basal side of the neural plate. The experimental elevation of mesoderm stiffness delayed neural plate bending, suggesting that low mesoderm stiffness mechanically supports neural tube closure. This study provides an example of mechanical interactions between tissues during large-scale morphogenetic movements.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 5","pages":"320-328"},"PeriodicalIF":1.7,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12936","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141460539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel preparation for histological analyses of intraventricular macrophages in the embryonic brain 用于胚胎脑室内巨噬细胞组织学分析的新型制备方法。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2024-06-19 DOI: 10.1111/dgd.12935

Futoshi Murayama, Hisa Asai, Arya Kirone Patra, Hiroaki Wake, Takaki Miyata, Yuki Hattori

{"title":"A novel preparation for histological analyses of intraventricular macrophages in the embryonic brain","authors":"Futoshi Murayama, Hisa Asai, Arya Kirone Patra, Hiroaki Wake, Takaki Miyata, Yuki Hattori","doi":"10.1111/dgd.12935","DOIUrl":"10.1111/dgd.12935","url":null,"abstract":"Microglia colonize the brain starting on embryonic day (E) 9.5 in mice, and their population increases with development. We have previously demonstrated that some microglia are derived from intraventricular macrophages, which frequently infiltrate the pallium at E12.5. To address how the infiltration of intraventricular macrophages is spatiotemporally regulated, histological analyses detecting how these cells associate with the surrounding cells at the site of infiltration into the pallial surface are essential. Using two-photon microscopy-based in vivo imaging, we demonstrated that most intraventricular macrophages adhere to the ventricular surface. This is a useful tool for imaging intraventricular macrophages maintaining their original position, but this method cannot be used for observing deeper brain regions. Meanwhile, we found that conventional cryosection-based and naked pallial slice-based observation resulted in unexpected detachment from the ventricular surface of intraventricular macrophages and their mislocation, suggesting that previous histological analyses might have failed to determine their physiological number and location in the ventricular space. To address this, we sought to establish a methodological preparation that enables us to delineate the structure and cellular interactions when intraventricular macrophages infiltrate the pallium. Here, we report that brain slices pretreated with agarose-embedding maintained adequate density and proper positioning of intraventricular macrophages on the ventricular surface. This method also enabled us to perform the immunostaining. We believe that this is helpful for conducting histological analyses to elucidate the mechanisms underlying intraventricular macrophage infiltration into the pallium and their cellular properties, leading to further understanding of the process of microglial colonization into the developing brain.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 5","pages":"329-337"},"PeriodicalIF":1.7,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12935","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141421768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An analysis of semaphorin-mediated cellular interactions in the Caenorhabditis elegans epidermis using the IR-LEGO single-cell gene induction system 利用 IR-LEGO 单细胞基因诱导系统分析半角蛋白介导的秀丽隐杆线虫表皮细胞相互作用

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2024-05-18 DOI: 10.1111/dgd.12925

Motoshi Suzuki, Shin Takagi

{"title":"An analysis of semaphorin-mediated cellular interactions in the Caenorhabditis elegans epidermis using the IR-LEGO single-cell gene induction system","authors":"Motoshi Suzuki, Shin Takagi","doi":"10.1111/dgd.12925","DOIUrl":"10.1111/dgd.12925","url":null,"abstract":"One of the major functions of the semaphorin signaling system is the regulation of cell shape. In the nematode Caenorhabditis elegans, membrane-bound semaphorins SMP-1/2 (SMPs) regulate the morphology of epidermal cells via their receptor plexin, PLX-1. In the larval male tail of the SMP-PLX-1 signaling mutants, the border between two epidermal cells, R1.p and R2.p, is displaced anteriorly, resulting in the anterior displacement of the anterior-most ray, ray 1, in the adult male. To elucidate how the intercellular signaling mediated by SMPs regulates the position of the intercellular border, we performed mosaic gene expression analyses by using infrared laser-evoked gene operator (IR-LEGO). We show that PLX-1 expressed in R1.p and SMP-1 expressed in R2.p are required for the proper positioning of ray 1. The result suggests that SMP signaling promotes extension, rather than retraction, of R1.p. This is in contrast to a previous finding that SMPs mediate inhibition of cell extension of vulval precursor cells, another group of epidermal cells of C. elegans, indicating the context dependence of cell shape control via the semaphorin signaling system.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 5","pages":"308-319"},"PeriodicalIF":1.7,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12925","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140960844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0