Development Growth & Differentiation最新文献_第3页

A Simple Method for RNA-Seq of Manually Isolated Chromatophores in Oryzias Fishes 一种人工分离米子鱼色素体的简易RNA-Seq方法。

IF 1 4区生物学

Development Growth & Differentiation Pub Date : 2026-03-10 DOI: 10.1111/dgd.70044

Makoto Goda, Asuka Miyagi, Keisuke Sugiwaka, Masakatsu Watanabe, Manabu Bessho-Uehara, Masahiko Hibi, Atsushi Toyoda, Rieko Tanaka, Kawilarang W. A. Masengi, Kazunori Yamahira, Satoshi Ansai, Hisashi Hashimoto

{"title":"A Simple Method for RNA-Seq of Manually Isolated Chromatophores in Oryzias Fishes","authors":"Makoto Goda, Asuka Miyagi, Keisuke Sugiwaka, Masakatsu Watanabe, Manabu Bessho-Uehara, Masahiko Hibi, Atsushi Toyoda, Rieko Tanaka, Kawilarang W. A. Masengi, Kazunori Yamahira, Satoshi Ansai, Hisashi Hashimoto","doi":"10.1111/dgd.70044","DOIUrl":"10.1111/dgd.70044","url":null,"abstract":"RNA sequencing (RNA-seq) has become an essential tool for analyzing gene expression and exploring cell type–specific transcriptomes. However, sample preparation and quality control remain challenging, as current approaches typically rely on dissecting tissues containing mixed cell populations or using flow cytometry to isolate fluorescently labeled cells. Here we present a simple and reliable method for RNA-seq of chromatophores (pigment cells) by manually isolating cells based on their natural pigmentation. We analyzed four chromatophore types—melanophores, xanthophores, iridophores, and leucophores—in medaka (Oryzias latipes). Remarkably, as few as 100 cells per type yielded reasonably high-quality transcriptomes sufficient to identify differentially expressed genes (DEGs). Furthermore, this method was successfully applied to a non-model medaka species, O. woworae, which shares the same four chromatophore types. Our approach enables efficient, low-cost, and cross-species transcriptome analysis of chromatophores without requiring transgenic markers or flow cytometry.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 3","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12973483/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147391681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome Comparison Between the Cultured and In Vivo Chick Primordial Germ Cells by SMART-Seq-Based Single-Cell RNA Sequencing 基于smart - seq单细胞RNA测序的培养和体内鸡原始生殖细胞转录组比较

IF 1 4区生物学

Development Growth & Differentiation Pub Date : 2026-03-03 DOI: 10.1111/dgd.70049

Yoshiki Hayashi, Atsushi Doi, Hiroko Iikawa, Hirokazu Kimijima, Yutaka Suzuki, Akinori Kanai, Hideki Hirakawa, Daisuke Saito

{"title":"Transcriptome Comparison Between the Cultured and In Vivo Chick Primordial Germ Cells by SMART-Seq-Based Single-Cell RNA Sequencing","authors":"Yoshiki Hayashi, Atsushi Doi, Hiroko Iikawa, Hirokazu Kimijima, Yutaka Suzuki, Akinori Kanai, Hideki Hirakawa, Daisuke Saito","doi":"10.1111/dgd.70049","DOIUrl":"10.1111/dgd.70049","url":null,"abstract":"Primordial germ cells (PGCs), the precursors of the germline, have unique cellular characteristics to undergo long-distance migration to the embryonic gonads and have the potential to differentiate into somatic cells. Among the animal models studying PGC development, the chicken PGCs are an ideal model, since it is a rare model in which long-term PGC cultivation is applicable. Although the cultural applicability of chicken PGC makes it attractive for revealing the PGC character and its developmental processes, some differences from in vivo PGCs are known, such as the remarkable upregulation of cell proliferation and a lesser ability to reach the gonads. Understanding these differences at the molecular level is crucial. To this end, we first performed SMART-seq-based single-cell RNA sequencing to compare transcriptomes between in vivo PGCs and cultured PGCs. Our results revealed that PGC cultivation causes a shift from a MYC-dependent to a MYCN-dependent gene regulatory network (GRN) in PGCs, suggesting that this reprogramming contributes to the acquisition of proliferation ability and stem cell characteristics in cultured PGCs. Additionally, our results suggest that the MYCN-dependent GRN increases the risk of somatic differentiation, particularly in neural fate, in cultured PGCs. In addition, our transcriptome analysis identified a new cell population that shows molecular characteristics of germline-biased undifferentiated cells. Thus, our study provides fundamental molecular information to understand both the effects of PGC cultivation and the developmental process of chicken PGCs.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 3","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13001537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147349721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Heat Shock Protein Expression and Its Application to Temporal Gene Expression in the Nematode Pristionchus pacificus 太平洋竖线虫热休克蛋白表达特征及其在时间基因表达中的应用。

IF 1 4区生物学

Development Growth & Differentiation Pub Date : 2026-02-15 DOI: 10.1111/dgd.70045

Yuuki Ishita, Hirokuni Hiraga, Takahiro Chihara, Misako Okumura

{"title":"Characterization of Heat Shock Protein Expression and Its Application to Temporal Gene Expression in the Nematode Pristionchus pacificus","authors":"Yuuki Ishita, Hirokuni Hiraga, Takahiro Chihara, Misako Okumura","doi":"10.1111/dgd.70045","DOIUrl":"10.1111/dgd.70045","url":null,"abstract":"Temporal gene expression systems are widely used to examine gene functions at specific developmental stages. The heat-inducible gene expression system, which uses a heat shock promoter with evolutionarily conserved heat shock elements, is used in temporal gene expression systems in many organisms. The nematode, Pristionchus pacificus, is a satellite model system comparable to Caenorhabditis elegans, with unique developmental traits but lacking genetic tools for assessing temporal gene expression. To establish a temporal gene expression system in P. pacificus, we investigated the genes that were highly induced by heat shock. RNA-sequencing analysis revealed many differentially expressed genes after a 2-h heat shock event. One of the highly induced genes, PPA12242, is an ortholog of C. elegans hsp-16.41, and transgenic animals harboring a reporter system have shown that the genomic fragment upstream of this gene can induce gene expression in response to heat shock. Using the PPA12242 promoter, gene expression can be induced at all larval stages, and some phenotypes appear to be vulnerable to heat stress. Taken together, we identified a potential heat shock promoter in P. pacificus that is applicable to the temporal gene expression system of this species.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 2","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12907538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146202926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heat-Shock-Inducible Gene Expression Systems in Amphibians: Utility in Regeneration and Metamorphosis Studies 两栖动物热休克诱导基因表达系统：在再生和变态研究中的应用。

IF 1 4区生物学

Development Growth & Differentiation Pub Date : 2026-02-15 DOI: 10.1111/dgd.70048

Ayumi Yamato, Koga Fujita, Mana Yoshida, Chikato Yamamoto, Koudai Abe, Makoto Suzuki, Aiko Kawasumi-Kita, Joe Sakamoto, Yasuhiro Kamei, Hajime Ogino, Yasutaka Hanada, Hitoshi Yokoyama

{"title":"Heat-Shock-Inducible Gene Expression Systems in Amphibians: Utility in Regeneration and Metamorphosis Studies","authors":"Ayumi Yamato, Koga Fujita, Mana Yoshida, Chikato Yamamoto, Koudai Abe, Makoto Suzuki, Aiko Kawasumi-Kita, Joe Sakamoto, Yasuhiro Kamei, Hajime Ogino, Yasutaka Hanada, Hitoshi Yokoyama","doi":"10.1111/dgd.70048","DOIUrl":"10.1111/dgd.70048","url":null,"abstract":"<div>\u0000 \u0000 Amphibians exhibit two remarkable biological phenomena: regeneration and metamorphosis. The ability to regenerate damaged body parts, such as the limbs, and to remodel organs—such as tail resorption during metamorphosis—is both fascinating and enigmatic. However, until recently, it has been difficult to manipulate gene expression in amphibians after embryogenesis, hindering molecular studies of these processes. Over the past two decades, the development of a simple and reproducible gene expression method—the heat-shock-inducible system—has helped overcome this limitation. This system involves generating transgenic animals carrying gene(s) of interest under the control of a heat shock promoter, typically the hsp70 promoter, followed by heat shock treatment to induce expression. Recent advancements have enabled not only the application of heat shock to the whole body but also spatially restricted gene induction in specific cell populations. In particular, laser irradiation allows for highly precise gene activation and lineage tracing, even at the single-cell level. One current limitation of this system is unintended “leaky” gene expression in the absence of heat shock. The recent availability of an alternative inducible system, Tet-on, in amphibians holds promise for overcoming this drawback and achieving tighter control of gene expression. In this review, we discuss potential refinements to the heat-shock-inducible system—including improvements in laser irradiation techniques and optimization of heat shock promoters (e.g., hsp70 promoter)—to address current limitations, and explore how this system may become an even more powerful tool for studying regeneration and metamorphosis in amphibians.\u0000 </div>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 2","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146203401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Thyroid Hormone Receptor α Gene Knockout on Hindlimb and Intestinal Development in Pre-Metamorphic Xenopus tropicalis Larvae 甲状腺激素受体α基因敲除对热带爪蟾后肢和肠道发育的影响。

IF 1 4区生物学

Development Growth & Differentiation Pub Date : 2026-02-05 DOI: 10.1111/dgd.70043

Shuhei Ogawa, Keisuke Nakajima, Nobuaki Furuno, Ichiro Tazawa

{"title":"Effects of Thyroid Hormone Receptor α Gene Knockout on Hindlimb and Intestinal Development in Pre-Metamorphic Xenopus tropicalis Larvae","authors":"Shuhei Ogawa, Keisuke Nakajima, Nobuaki Furuno, Ichiro Tazawa","doi":"10.1111/dgd.70043","DOIUrl":"10.1111/dgd.70043","url":null,"abstract":"<div>\u0000 \u0000 During anuran metamorphosis, rapid and extensive morphological transformations occur throughout the body, and these changes are triggered by the thyroid hormone. The thyroid hormone receptor (TR) is a nuclear receptor that is present in all vertebrates. TR binds specific DNA sequences to repress target genes in the absence of ligand and to activate them when ligand-bound. This dual regulatory function has been proposed to underlie the rapid pace of anuran metamorphosis. One TR subtype, TRα, suppresses hindlimb (HL) development in pre-metamorphic Xenopus tropicalis. However, the genes repressed by TRα remain unidentified, and it is unclear whether TRα-mediated developmental suppression occurs in specific organs. This study aimed to identify HL genes regulated by TRα during developmental suppression and to determine whether this suppression is widespread in the pre-metamorphic tadpole. We analyzed temporal changes in morphology and gene expression in the HL buds and intestines of TRα-knockout (KO) pre-metamorphic X. tropicalis tadpoles. HL buds appeared earlier in KO tadpoles than in the wild type. Whole-mount in situ hybridization showed that the interval from fertilization to initial expression of shh was shorter in KO HLs. However, the expression pattern in HL buds with comparable morphology was essentially identical between genotypes. On the other hand, there was no significant acceleration in the growth of the intestine or body (from snout to vent) of the KO tadpoles. Our findings suggest that unliganded TRα delays the onset of HL development prior to metamorphosis.\u0000 </div>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 2","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146127320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Maternal Organ Growth: How the Adult Intestine Remodels During Pregnancy and Lactation” 更正“母体器官生长：妊娠和哺乳期成人肠道如何重塑”。

IF 1 4区生物学

Development Growth & Differentiation Pub Date : 2026-02-05 DOI: 10.1111/dgd.70046

引用次数: 0

Conserved Interhemispheric Morphogenesis in Amniotes Preceded the Evolution of the Corpus Callosum 羊膜动物保守的半球间形态发生先于胼胝体的进化。

IF 1 4区生物学

Development Growth & Differentiation Pub Date : 2026-01-20 DOI: 10.1111/dgd.70041

Ryota Noji, Mari Kaneko, Takaya Abe, Hiroshi Kiyonari, Yukihiro Nishikawa, Takuma Kumamoto, Hitoshi Gotoh, Chiaki Ohtaka-Maruyama, Katsuhiko Ono, Tatsuya Yoshizawa, Tadashi Nomura

{"title":"Conserved Interhemispheric Morphogenesis in Amniotes Preceded the Evolution of the Corpus Callosum","authors":"Ryota Noji, Mari Kaneko, Takaya Abe, Hiroshi Kiyonari, Yukihiro Nishikawa, Takuma Kumamoto, Hitoshi Gotoh, Chiaki Ohtaka-Maruyama, Katsuhiko Ono, Tatsuya Yoshizawa, Tadashi Nomura","doi":"10.1111/dgd.70041","DOIUrl":"10.1111/dgd.70041","url":null,"abstract":"<div>\u0000 \u0000 The corpus callosum (CC) is the large axon bundle connecting the telencephalic hemispheres. The CC is formed exclusively in placental mammals, and the lack of comparable structures in other amniotes obscures the evolutionary origin of the CC. We here demonstrate that interhemispheric remodeling, a prior developmental step for CC formation, is highly conserved in nonmammalian amniotes, such as reptiles and birds. In these animal groups, the spatio-temporal dynamics of interhemispheric remodeling are tightly connected with distinct commissural formations. We observed a high degree of similarity between the mammalian CC and reptilian rostral pallial commissure (RPC) and significant modifications in the avian pallial projection. Furthermore, we determined that Satb2 plays crucial roles in interhemispheric remodeling, which is associated with proper formation of both the CC and RPC in mice and geckoes, via the use of Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-mediated gene targeting. Our findings suggest that developmental mechanisms for midline remodeling were already present in the common ancestor of amniotes, which contributed to the evolution of eutherian-specific CC formation.\u0000 </div>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146013399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Zinc Finger Protein Znf281 Is Essential for the Formation of Neural Tissue in Xenopus Embryos 锌指蛋白Znf281对爪蟾胚胎神经组织的形成至关重要。

IF 1 4区生物学

Development Growth & Differentiation Pub Date : 2026-01-14 DOI: 10.1111/dgd.70040

Ryota Koike, Makoto Nakamura, Kimiko Takebayashi-Suzuki, Atsushi Suzuki

{"title":"The Zinc Finger Protein Znf281 Is Essential for the Formation of Neural Tissue in Xenopus Embryos","authors":"Ryota Koike, Makoto Nakamura, Kimiko Takebayashi-Suzuki, Atsushi Suzuki","doi":"10.1111/dgd.70040","DOIUrl":"10.1111/dgd.70040","url":null,"abstract":"<div>\u0000 \u0000 Zinc finger protein 281 (Znf281) plays important roles in human malignancies, stem cell pluripotency, and placental and embryonic development. However, the function of Znf281 during early neural development remains unclear. Here, we investigated the role of Znf281 in the formation of neural tissue in Xenopus embryos. znf281 transcripts are expressed in the animal hemisphere of the embryo at the blastula and gastrula stages and gradually localize in neural tissue after gastrulation. Overexpression of Znf281 induces neural tissue with anterior–posterior patterning and inhibits epidermal differentiation in ectodermal explants and embryos. Mechanistically, Znf281 reduces the levels of phosphorylated Smad1/5/8 proteins, the downstream effectors of bone morphogenetic protein (BMP) signaling, to promote neural development. Moreover, knockdown of Znf281 in embryos results in the reduced expression of neural markers, indicating that Znf281 is required for early neural development. These results suggest that Znf281 plays an important role in the establishment of the central nervous system by modulating BMP signaling during vertebrate embryogenesis.\u0000 </div>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145971489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of cyp19a1a and cyp19a1b Knockout on Germ Cell Kinetics During Gonadal Sex Differentiation in Medaka 敲除cyp19a1a和cyp19a1b对Medaka性腺性别分化过程中生殖细胞动力学的影响。

IF 1 4区生物学

Development Growth & Differentiation Pub Date : 2026-01-10 DOI: 10.1111/dgd.70038

Yuta Sakai-Yamada, Taijun Myosho, Tohru Kobayashi

{"title":"Effects of cyp19a1a and cyp19a1b Knockout on Germ Cell Kinetics During Gonadal Sex Differentiation in Medaka","authors":"Yuta Sakai-Yamada, Taijun Myosho, Tohru Kobayashi","doi":"10.1111/dgd.70038","DOIUrl":"10.1111/dgd.70038","url":null,"abstract":"<div>\u0000 \u0000 In medaka (Oryzias latipes), the first morphological sex difference is germ cell number before hatching, which is determined by the dmy gene on the Y chromosome. This study aimed to clarify whether zygotically synthesized estrogen influences the kinetics of germ cell number during early gonadal sex differentiation. We established disruptive mutants of the estrogen-synthesizing enzyme aromatase by knocking out cyp19a1a (Δcyp19a1a) and cyp19a1b (Δcyp19a1b) as well as double disruptive mutants (Δcyp19a1s DKO) from each individual knockout using CRISPR/Cas9. Δcyp19a1s DKO XY and XX adult fish at 90 days post-hatching (dph) exhibited basal levels of estradiol-17β. At hatching (0 dph: stage 39), WT XX fry had significantly more germ cells than WT XY fry, and gonial cells were the most advanced germ cell stage across both sexes. Germ cell number and gonadal histology in Δcyp19a1s DKO mutants resembled those of WT fry. At 10 dph, germ cell number and gonadal histology were also similar between WT and Δcyp19a1s XY fry. In Δcyp19a1s DKO XX fry, diplotene oocytes and the total number of germ cells were significantly lower compared with WT. Exposure to 17α-ethynylestradiol rescued the reduction in diplotene oocytes in Δcyp19a1s DKO mutants to levels comparable to the control, resulting in the rescue of total germ cell number. Overall, our findings suggest that zygotically synthesized estrogen does not affect sex differences in germ cell number as the initial morphological sex difference but partly facilitates the differentiation from pachytene to diplotene oocytes.\u0000 </div>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145945609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sir John B. Gurdon (1933–2025) 约翰·b·戈登爵士（1933-2025）

IF 1 4区生物学

Development Growth & Differentiation Pub Date : 2026-01-10 DOI: 10.1111/dgd.70039

Makoto Asashima

引用次数: 0