Development Growth & Differentiation最新文献

{"title":"The property of larval cells of the scleractinian coral, Acropora tenuis, deduced from in vitro cultured cells.","authors":"Kaz Kawamura, Satoko Sekida, Koki Nishitsuji, Noriyuki Satoh","doi":"10.1111/dgd.70000","DOIUrl":"https://doi.org/10.1111/dgd.70000","url":null,"abstract":"<p><p>In previous studies, we have established approximately 15 cultured cell-lines derived from planula larvae of Acropora tenuis. Based on their morphology and behavior, these cells were classified into three types, flattened amorphous cells (FAmCs), vacuolated adherent cells (VAdCs), and small smooth cells (SSmCs). FAmCs include fibroblast-like cells and spherical, brilliant brown cells (BBrCs), which are transformable to each other. To examine the larval origin of the three cell types, we raised antibodies: anti-AtMLRP2 that appears to recognize FAmC, anti-AtAHNAK for BBrC, anti-AtSOMP5 and anti-AtEndoG for SSmC, and anti-AtGal and anti-AtFat4 for VAdC, respectively. Anti-AtMLRP2 antibody stained in vivo stomodeum and neuroblast-like cells embedded in larval ectoderm around the aboral pole. Anti-AtAHNAK antibody stained neuron-like and neuroblast-like cells, both of which were also stained with neuron-specific tubulin β-3 antibody. These results suggest that in vitro BBrCs and in vivo neuroblast-like cells share neuronal properties in common. Two antibodies for SSmCs, anti-AtSOMP5 and anti-AtEndoG, stained larval ectoderm cells, suggesting that SSmCs have larval ectoderm properties. Two antibodies for VAdCs, anti-AtGal and anti-AtFat4, stained larval endoderm cells, suggesting that VAdCs have larval endoderm properties. Therefore, the in vitro cell lines appear to retain properties of the stomodeum, neuroblast, ectoderm, or endoderm. Each of them may be used in future investigations to reveal cellular and molecular properties of cell types of coral larvae, such as the potential for symbiosis.</p>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143469952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromosomal localization of PHOX2B during M-phase is disrupted in disease-associated mutants.","authors":"Yuki Sato, Shinichi Hayashi, Souichi Oe, Taro Koike, Yousuke Nakano, Ryohei Seki-Omura, Hikaru Iwashita, Yukie Hirahara, Masaaki Kitada","doi":"10.1111/dgd.70001","DOIUrl":"https://doi.org/10.1111/dgd.70001","url":null,"abstract":"<p><p>In the M-phase, the nuclear membrane is broken down, nucleosomes are condensed as mitotic chromosomes, and transcription factors are generally known to be dislocated from their recognition sequences and dispersed to the cytoplasm. However, some transcription factors have recently been reported to remain on mitotic chromosomes and facilitate the rapid re-activation of the target genes in early G1-phase. Paired-like homeobox 2B (PHOX2B) is a transcription factor exhibiting chromosomal localization during M-phase. PHOX2B mutations are associated with congenital central hypoventilation syndrome, Hirschsprung disease, and neuroblastoma. In this study, we investigated PHOX2B chromosomal localization during M-phase through immunostaining and fluorescence recovery after photobleaching analysis to determine whether the chromosomal localization of disease-associated PHOX2B mutants is altered during M-phase. Missense mutations in the homeodomain and the frameshift mutation in the C-terminal domain disrupted the chromosomal localization of PHOX2B in M-phase, leading to its dispersion in the cell. Furthermore, a PHOX2B mutant with polyalanine expansion showed a line-shaped localization to the restricted region of mitotic chromosomes. Our findings suggest an association between the disease-associated mutations and defective chromosomal localization of transcription factors during M-phase. Further investigations of PHOX2B chromosomal localization during M-phase could reveal pathogenic mechanisms of such diseases.</p>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mild cryoinjury in zebrafish fin induces regenerative response without blastema formation.","authors":"Takafumi Yoshida, Atsushi Kawakami","doi":"10.1111/dgd.12962","DOIUrl":"https://doi.org/10.1111/dgd.12962","url":null,"abstract":"<p><p>Previous studies have shown that tissue regeneration induces expression of genes that play important roles in regeneration. Recently, several studies have identified regeneration-response enhancers (RREs) that activate gene expression by tissue injury. Particularly, we showed that RREs contain two transcription factor-binding motifs: a bHLH transcription factor-binding motif, an E-box, and an AP-1/bZIP transcription factor-binding motif, a 12-O-Tetradecanoylphorbol 13-acetate response element (TRE). However, the triggers and subsequent signals generated by injury are still unclear. In this study, we analyzed RRE activation using various injury models. Although inter-ray incisions and skin exfoliation injuries did not activate RREs or regeneration genes, the fin puncture injury activated RREs and several regeneration-response genes. After fin puncture injury, msxc was activated only on the proximal side of the hole where blastema-like tissue was formed, whereas RREs, junbb, and fibronectin 1b (fn1b) were activated on both the proximal and distal sides, implying that activation of RREs, junbb, and fn1b is independent of blastema formation. Here, we also established a mild cryoinjury method. After this injury, transient vascular destruction, an increase in cell death, and an accumulation of myeloid cells were observed; however, no major morphological damage was observed. Importantly, msxc was not induced by cryoinjury, whereas fn1b, junbb, and 1.8 k RRE (-1.8 kb promoter of fn1b) were activated, suggesting that cryoinjury induces the responses of fn1b, junbb, and 1.8 k RRE without forming the blastema. Thus, our study shows that the cryoinjury model and the RRE transgenic (Tg) zebrafish may provide a useful platform for exploring injury signals.</p>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding disorders of the human nervous system: How fish models reveal disease mechanisms from single molecules to behavior (part 2)","authors":"Christina Lillesaar,&nbsp;William Norton,&nbsp;Daniel Liedtke,&nbsp;Sachiko Tsuda","doi":"10.1111/dgd.12951","DOIUrl":"10.1111/dgd.12951","url":null,"abstract":"<p>The usefulness of zebrafish for understanding the human nervous system is exemplified by the articles in part 1. The virtual special issue part 2 not only covers more work using this well-established species, but also highlights that other fish species may serve as alternative or more appropriate models, due to unique biological or evolutionary characteristics, to explore genetic and molecular mechanisms of neurological and psychiatric disorders.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"67 1","pages":"4-5"},"PeriodicalIF":1.7,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143034647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regeneration of Lumbriculus variegatus requires post-amputation production of reactive oxygen species","authors":"Freya R. Beinart,&nbsp;Kathy Gillen","doi":"10.1111/dgd.12961","DOIUrl":"10.1111/dgd.12961","url":null,"abstract":"<p>Animals vary in their ability to replace body parts lost to injury, a phenomenon known as restorative regeneration. Uncovering conserved signaling steps required for regeneration may aid regenerative medicine. Reactive oxygen species (ROS) are necessary for proper regeneration in species across a wide range of taxa, but it is unknown whether ROS are essential for annelid regeneration. As annelids are a widely used and excellent model for regeneration, we sought to determine whether ROS play a role in the regeneration of the highly regenerative annelid, <i>Lumbriculus variegatus</i>. Using a ROS-sensitive fluorescent probe we observed ROS accumulation at the wound site within 15 min after amputation; this ROS burst lessened by 6 h post-amputation. Chemical inhibition of this ROS burst delayed regeneration, an impairment that was partially rescued with exogenous ROS. Our results suggest that similar to other animals, annelid regeneration depends upon ROS signaling, implying a phylogenetically ancient requirement for ROS in regeneration.</p>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"67 2","pages":"104-112"},"PeriodicalIF":1.7,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12961","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"“Fly to New World”: Meeting report of the 16th Japanese Drosophila research conference (JDRC16)","authors":"Haruka Yoshizawa,&nbsp;Erina Kuranaga","doi":"10.1111/dgd.12959","DOIUrl":"10.1111/dgd.12959","url":null,"abstract":"<p>The 16th Japanese <i>Drosophila</i> Research Conference (JDRC16) was held at the Sendai International Center from September 17 to 19 2024. It had been 2 years since the last JDRC15 held in Nagoya. The conference brought together 231 researchers, including 22 researchers from overseas, creating a vibrant and diverse platform for scientific exchange. Prof. Shigeo Hayashi of RIKEN BDR delivered a keynote lecture, and his groundbreaking ideas and research captivated the audience. Over the 3 days, the conference featured 53 oral presentations across 11 sessions and 2 special sessions, as well as 128 poster presentations, all of which fostered stimulating discussions and the exchange of innovative ideas. The reception provided an additional opportunity for researchers to engage in meaningful dialogue while enjoying Sendai's renowned specialties. Held under clear autumn skies in a great nature along the river, this conference painted a beautiful contrast to the heated discussions in the venue. Consequently, this conference fully contributed to the mission proffered by Prof. Hayashi, “Fly to New World,” expanding the insights gained from flies into new and unexplored scientific areas.</p>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"67 2","pages":"43-46"},"PeriodicalIF":1.7,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The transmembrane protein TMEM196 controls cell proliferation and determines the floor plate cell lineage","authors":"Yumi Matsumoto,&nbsp;Seiichi Tamaru,&nbsp;Xing Chen,&nbsp;Takuma Shinozuka,&nbsp;Yuichi Sakumura,&nbsp;Noriaki Sasai","doi":"10.1111/dgd.12960","DOIUrl":"10.1111/dgd.12960","url":null,"abstract":"<p>The neural tube, the embryonic precursor to the vertebrate central nervous system, comprises distinct progenitor and neuronal domains, each with specific proliferation programs. In this study, we identified TMEM196, a novel transmembrane protein that plays a crucial role in regulating cell proliferation in the floor plate in chick embryos. TMEM196 is expressed in the floor plate, and its overexpression leads to reduced cell proliferation without affecting the pattern formation of the neural tube. We also established the floor plate differentiation protocol of the mouse embryonic stem cells, and analyzed the function of TMEM196 with this system. Mutating the <i>Tmem196</i> gene does not alter cell division and overall differentiation remains unchanged within the neural cells. However, TMEM196 inhibits Wnt signaling, and <i>Tmem196</i> mutant cells exhibit aberrant paraxial mesoderm differentiation, suggesting that TMEM196 selects the floor plate cell fate at the binary decision of the neuromesodermal cells. These findings highlight TMEM196 as a key regulator of both cell proliferation and floor plate determination, contributing to proper regionalization during embryogenesis.</p>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"67 2","pages":"94-103"},"PeriodicalIF":1.7,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early development of the calcified exoskeleton of the polyplacophoran mollusk, with insight into the evolutionary history of shell plates and spicules","authors":"Hiroki Yoshikawa,&nbsp;Yoshiaki Morino,&nbsp;Hiroshi Wada","doi":"10.1111/dgd.12956","DOIUrl":"10.1111/dgd.12956","url":null,"abstract":"<p>Recent molecular phylogenetic studies have raised two questions about the evolutionary history of the calcified exoskeleton of mollusks. The first question concerns the homology of the two types of skeleton: whether spicules and shell plates share an evolutionary origin. The second question is the homology of the shell plates between chitons and other mollusks, including gastropods and bivalves. To gain insight into these questions, we examined the early development of shell plates and spicules in chitons. We identified several developmental genes that are involved in both shell plates and spicules, suggesting that spicules and shell plates share a common evolutionary origin. We also found that subpopulations of the dorsal shell field (the ridge and the plate field) have specific gene expression profiles. The differential gene expression of the ridge and plate field is not identical to the profiles of the zones of the gastropod shell field. This observation may suggest an independent evolutionary origin of the shell plates in chitons and gastropods.</p>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"67 2","pages":"47-54"},"PeriodicalIF":1.7,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142958285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sonic Hedgehog signaling regulates the optimal differentiation pace from early-stage mesoderm to cardiogenic mesoderm in mice","authors":"Satoshi Inoue,&nbsp;Moe Nosetani,&nbsp;Yoshiro Nakajima,&nbsp;Shinichiro Sakaki,&nbsp;Hiroki Kato,&nbsp;Rie Saba,&nbsp;Naoki Takeshita,&nbsp;Kosuke Nishikawa,&nbsp;Atsuko Ueyama,&nbsp;Kazuhiko Matsuo,&nbsp;Masaki Shigeta,&nbsp;Daisuke Kobayashi,&nbsp;Tomoko Iehara,&nbsp;Kenta Yashiro","doi":"10.1111/dgd.12955","DOIUrl":"10.1111/dgd.12955","url":null,"abstract":"<p><i>Sonic Hedgehog</i> (<i>Shh</i>), encoding an extracellular signaling molecule, is vital for heart development. <i>Shh</i> null mutants show congenital heart disease due to left–right asymmetry defects stemming from functional anomaly in the midline structure in mice. <i>Shh</i> signaling is also known to affect cardiomyocyte differentiation, endocardium development, and heart morphogenesis, particularly in second heart field (SHF) cardiac progenitor cells that contribute to the right ventricle, outflow tract, and parts of the atrium. Despite extensive studies, our understanding remains incomplete. Notably, <i>Shh</i> signaling is suggested to promote cardiac differentiation, while paradoxically preventing premature differentiation of SHF progenitors. In this study, we elucidate the role of <i>Shh</i> signaling in the earliest phase of cardiac differentiation. Our meta-analysis of single-cell RNA sequencing suggests that cardiogenic nascent mesoderm cells expressing the bHLH transcription factor <i>Mesp1</i> interact with axial mesoderm via Hh signaling. Activation of Hh signaling using a Smoothened agonist delayed or suppressed the differentiation of primitive streak cells expressing T-box transcription factor <i>T</i> to <i>Mesp1</i>⁺ nascent mesoderm cells both in vitro and ex vivo. Conversely, inhibition of Hh signaling by cyclopamine facilitated cardiac differentiation. The reduction of <i>Eomes</i>, an inducer of <i>Mesp1</i>, by Hh signaling appears to be the underlying mechanism of this phenomenon. Our data suggest that SHH secreted from axial mesoderm inhibits premature differentiation of <i>T</i>⁺ cells to <i>Mesp1</i>⁺ nascent mesoderm cells, thereby regulating the pace of cardiac differentiation. These findings enhance our comprehension of <i>Shh</i> signaling in cardiac development, underscoring its crucial role in early cardiac differentiation.</p>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"67 2","pages":"75-84"},"PeriodicalIF":1.7,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12955","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142958326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative approach to the microstructure in the carpus and tarsus in anurans","authors":"Marissa Fabrezi,&nbsp;Julio César Cruz","doi":"10.1111/dgd.12957","DOIUrl":"10.1111/dgd.12957","url":null,"abstract":"<p>Despite the significant literature about morphological features of limb skeletons involved in tetrapod limb evolution, some questions about carpal and tarsal elements remain. In anurans, the ecomorphological and biomechanical approaches studied long hind limbs (to jump) and forelimbs (to land) and emphasized the role of the long bones in locomotion but disregarded what happens with the nodular elements of the carpus and tarsus. Here, we present a comparative study of nodular elements of the carpus and tarsus in anurans based on whole-mount specimens stained with Alcian Blue (cartilage) and Alizarin Red S (bone and calcified cartilage). The sample comprises 113 species belonging to 33 anuran families and postmetamorphic series in selected species. Further, we analyze the histology of the carpus and tarsus in individuals of nine species. In most anurans, the carpal and tarsal elements are cartilaginous in adult stages. The cartilaginous matrix may present different degrees of calcification. Few taxa present truly ossified carpals and tarsals with marrow cavity, blood cells, and hematopoietic cells. Interpretation of the interspecific variation in the carpus and tarsus skeletons on the most recent anuran phylogeny suggests that the delayed ossification of carpals and tarsals has evolved in derived lineages (e.g. Pelobatoidea and Neobatrachia).</p>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"67 2","pages":"55-74"},"PeriodicalIF":1.7,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142958284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}