Development Growth & Differentiation最新文献

A Continuous In Vitro Culture Protocol for Mouse Embryos From the Zygote to the Egg Cylinder Stage 小鼠胚胎从受精卵到卵柱期的连续体外培养方案

IF 1.5 4区生物学

Development Growth & Differentiation Pub Date : 2026-10-07 DOI: 10.1111/dgd.70070

Rui Shibata, Hiromi Shimojo, Hiroshi Sasaki

{"title":"A Continuous In Vitro Culture Protocol for Mouse Embryos From the Zygote to the Egg Cylinder Stage","authors":"Rui Shibata, Hiromi Shimojo, Hiroshi Sasaki","doi":"10.1111/dgd.70070","DOIUrl":"https://doi.org/10.1111/dgd.70070","url":null,"abstract":"<div>\u0000 \u0000 Mouse embryos undergo drastic morphological changes during implantation, but analysis of this process has been challenging because it occurs within the uterus. Various in vitro culture systems supporting development beyond implantation have been established since the 1970s. More recently, 3D-geec, which supports embryos from the peri-implantation to egg cylinder stages while preserving their three-dimensional organization, was developed as a useful platform for studying this critical period. Combining 3D-geec with genome editing in zygotes or experimental manipulation of embryos should further facilitate mechanistic studies of these stages. In this study, to enable such approaches, we established an in vitro culture protocol that supports continuous development of mouse embryos from the zygote to the egg cylinder stage. Embryos cultured from the zygote to the late blastocyst stage in KSOMAA medium were not compatible with 3D-geec. These embryos exhibited reduced cell numbers, delayed development, and reduced mTORC1 activity, which regulates cell growth and proliferation. The use of Advanced IVC medium supplemented with 10% fetal bovine serum (FBS) during the blastocyst stage substantially rescued these defects, and the resulting late blastocysts underwent further development into egg cylinders in 3D-geec. Furthermore, genome-edited embryos generated from zygotes developed into egg cylinders using this protocol and were successfully subjected to time-lapse imaging during 3D-geec. Thus, this culture protocol enables continuous in vitro development from the zygote to the egg cylinder stage and provides a platform for studying gene function and cellular dynamics during peri-implantation and early postimplantation development.\u0000 </div>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 8","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149100039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ptbp1 Condensates With Phase Separation-Like Features in Cardiomyocyte Nuclei Regulate Splicing of Proliferative Genes in Neonatal Cardiomyocytes 心肌细胞核中具有相分离样特征的Ptbp1凝聚体调节新生儿心肌细胞中增殖基因的剪接

IF 1.5 4区生物学

Development Growth & Differentiation Pub Date : 2026-10-04 DOI: 10.1111/dgd.70069

Tai Sada, Akane Sakaguchi, Yoichi Shinkai, Reiko Nakagawa, Chiharu Tanegashima, Mitsutaka Kadota, Takefumi Kondo, Wataru Kimura

{"title":"Ptbp1 Condensates With Phase Separation-Like Features in Cardiomyocyte Nuclei Regulate Splicing of Proliferative Genes in Neonatal Cardiomyocytes","authors":"Tai Sada, Akane Sakaguchi, Yoichi Shinkai, Reiko Nakagawa, Chiharu Tanegashima, Mitsutaka Kadota, Takefumi Kondo, Wataru Kimura","doi":"10.1111/dgd.70069","DOIUrl":"https://doi.org/10.1111/dgd.70069","url":null,"abstract":"Cardiovascular diseases remain the leading cause of morbidity and mortality worldwide; however, effective clinical therapies that achieve myocardial regeneration are still lacking, underscoring the urgent need for novel therapeutic strategies. In this study, we identified an RNA-binding protein Ptbp1, a key component of RNA metabolism, as a critical regulator of cardiomyocyte proliferation via the formation of biomolecular condensates. We found that Ptbp1 exhibits granule-like structures in cardiomyocyte nuclei during fetal to early postnatal stages. These Ptbp1 foci are phase-separated liquid droplets formed via its multivalent RNA binding domains. Functional analyses revealed that Ptbp1 is essential for cardiomyocyte proliferation in neonatal mouse primary culture assays. Furthermore, proximity labeling using a primary antibody-based approach combined with RIP-seq in highly purified cardiomyocyte populations demonstrated that these condensates are enriched with multiple proliferation-associated factors. Our findings identify a novel phase-separated condensate in cardiomyocytes and provide a new potential therapeutic target for cardiac regeneration.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 8","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.70069","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149099710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wnt5a Is Critical for the Maintenance of Cell Proliferation and Differentiation in Xenopus Tadpole Tail Regeneration. Wnt5a对维持蝌蚪爪尾再生细胞增殖和分化至关重要。

IF 1.5 4区生物学

Development Growth & Differentiation Pub Date : 2026-10-01 DOI: 10.1111/dgd.70071

Makoto Nakamura, Ryutaro Nishijima, Tatsuya Kyoda, Yuka Moriyama, Hitoshi Yoshida, Ryota Koike, Kimiko Takebayashi-Suzuki, Marko E Horb, Atsushi Suzuki

{"title":"Wnt5a Is Critical for the Maintenance of Cell Proliferation and Differentiation in Xenopus Tadpole Tail Regeneration.","authors":"Makoto Nakamura, Ryutaro Nishijima, Tatsuya Kyoda, Yuka Moriyama, Hitoshi Yoshida, Ryota Koike, Kimiko Takebayashi-Suzuki, Marko E Horb, Atsushi Suzuki","doi":"10.1111/dgd.70071","DOIUrl":"https://doi.org/10.1111/dgd.70071","url":null,"abstract":"Wnt signaling is essential for tissue and organ regeneration in animals such as amphibians, and several Wnt ligands are highly expressed in regenerating tissues. However, little is known about the specific role played by each ligand and their relative contribution to regeneration processes. Here, we show that Wnt5a plays critical roles in cell proliferation and differentiation during Xenopus tadpole tail regeneration. CRISPR/Cas9-mediated knock-out (KO) of the wnt5a gene in Xenopus tadpoles caused a delay in tail regeneration. Expression of wnt5a is induced after wound healing in regenerating tails and is positively regulated by the early injury response genes junb and inhba, which are required for the initiation of regenerative cell proliferation. Interestingly, in wnt5a KO tadpoles, cell proliferation initiated by JunB and InhibinβA was reduced at late stages of tail regeneration, suggesting an essential role of Wnt5a in the maintenance of regenerative cell proliferation. Moreover, differentiation of the notochord and neural tissue was also affected in the regenerating tails of wnt5a KO tadpoles. Our findings demonstrate that Wnt5a acts downstream of JunB and InhibinβA to maintain cell proliferation and that it also promotes differentiation in Xenopus tadpole tail regeneration.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 8","pages":"e70071"},"PeriodicalIF":1.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149220773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PDMS-Based Device for Long-Term Imaging of Gut Peristalsis by Suppressing Tissue Drift in Chicken Embryos 基于pdms的抑制鸡胚胎组织漂移的肠蠕动长期成像装置

IF 1.5 4区生物学

Development Growth & Differentiation Pub Date : 2026-09-25 DOI: 10.1111/dgd.70068

Masafumi Inaba, Koji Kawamura, Yoshiko Takahashi

{"title":"PDMS-Based Device for Long-Term Imaging of Gut Peristalsis by Suppressing Tissue Drift in Chicken Embryos","authors":"Masafumi Inaba, Koji Kawamura, Yoshiko Takahashi","doi":"10.1111/dgd.70068","DOIUrl":"https://doi.org/10.1111/dgd.70068","url":null,"abstract":"<div>\u0000 \u0000 Gut peristalsis is essential for the propulsion of luminal contents in the mature gut. Recent studies have suggested that embryonic peristalsis functions as a morphogenetic signal that regulates gut development. For these studies, long-term time-lapse analyses with ex vivo culture must be powerful to understand the dynamics of gut peristalsis. However, a cultured gut specimen undergoes a physical drifting within the microscopic field of view caused by repetitive contractions, preventing long-term quantitative analysis. Here, we developed a simple and inexpensive platform for long-term imaging of embryonic gut peristalsis using a polydimethylsiloxane (PDMS)-based holding device fabricated from a 3D-printed resin mold, which was prepared with a commercially available masked stereolithography printer. The resulting PDMS device successfully held in place a cultured cecum prepared from an embryonic day 10 chicken embryo for 24 h without interfering with intrinsic peristaltic movements and gut elongation. Long-term recordings enabled quantification of the origins, propagation direction, and propagation velocity of peristaltic waves for 24 h. Markedly, these analyses unveiled previously unrecognized differences in peristaltic activity between proximal and distal halves of cecum: while contractions occurred continuously in the proximal half, the distal half exhibited burst and quiescent phases that alternate with an approximately 30-min periodicity. The PDMS platform developed in this study provides a robust and accessible approach for investigating the long-term spatiotemporal dynamics of embryonic gut peristalsis, which will facilitate studies of peristalsis during gut morphogenesis.\u0000 </div>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 7","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149048337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental Fates of Progenitor/Undifferentiated Cells With Histone Trimethylation Tags in the Stony Coral, Acropora tenuis 带组蛋白三甲基化标签的石珊瑚祖细胞/未分化细胞的发育命运

IF 1.5 4区生物学

Development Growth & Differentiation Pub Date : 2026-09-02 DOI: 10.1111/dgd.70067

Kaz Kawamura, Satoko Sekida, Noriyuki Satoh

{"title":"Developmental Fates of Progenitor/Undifferentiated Cells With Histone Trimethylation Tags in the Stony Coral, Acropora tenuis","authors":"Kaz Kawamura, Satoko Sekida, Noriyuki Satoh","doi":"10.1111/dgd.70067","DOIUrl":"https://doi.org/10.1111/dgd.70067","url":null,"abstract":"The stony coral, Acropora tenuis, possesses progenitor/undifferentiated cells similar to other anthozoans; however, their developmental potential and fate remain obscure. The present study initially showed that nerve progenitors and undifferentiated-like cells were both tagged with histone H3 trimethylation at Lys 4 and Lys 27, suggesting that Acropora progenitor/undifferentiated cells might be multipotent. A rabbit anti-reverse transcriptase (anti-AtRTase) antibody recognized undifferentiated-like cells in the middle region of the ectodermal layer in embryos 60–85 h post-fertilization. At the larval stages, AtRTase signals were concentrated around the oral pole where the larval ectoderm invaginated to form the pharyngeal stomodeum. Undifferentiated-like cells, but not the apical-most glandular epithelium in the ectoderm, appeared to enter the pharynx. Consistent with this hypothesis, AtRTase signals were continuous from undifferentiated-like cells to the stomodeum. Nerve progenitors (neuroblasts) expressed nerve-specific tubulin beta-III (TUBB3). They differentiated into nerve cells and expressed a neuroblast differentiation-associated protein around the aboral pole of larvae. On the other hand, non-aboral neuroblasts expressed the autophagy-related protein 10 in addition to TUBB3 and developed into vacuolated hyaline cells (VHCs) that penetrated through the ectoderm apicobasally. The present study suggests strongly that ectodermal undifferentiated cells in A. tenuis have the potential to form the stomodeum. In contrast, nerve progenitors possess the dual potential to differentiate into nerve cells and VHCs, which appears to depend on aboral and non-aboral positions.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 7","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.70067","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148872068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revisiting Pluripotency Acquisition: A Unified Framework for Reprogramming Strategies 重新审视多能性获得：重编程策略的统一框架

IF 1.5 4区生物学

Development Growth & Differentiation Pub Date : 2026-08-17 DOI: 10.1111/dgd.70066

Anamika Datta, Kunimasa Ohta

{"title":"Revisiting Pluripotency Acquisition: A Unified Framework for Reprogramming Strategies","authors":"Anamika Datta, Kunimasa Ohta","doi":"10.1111/dgd.70066","DOIUrl":"https://doi.org/10.1111/dgd.70066","url":null,"abstract":"The traditional hierarchical view of totipotent cells generating diverse lineages of terminally differentiated cells was challenged by the discovery of induced pluripotent stem cells (iPSCs). This breakthrough demonstrated that the ectopic expression of four transcription factors, Oct4, Sox2, Klf4, and c-Myc (OSKM), can reprogram somatic cells to a pluripotent state. Since then, studies have expanded into diverse reprogramming strategies employing different cell types, factor combinations, delivery methods, and microenvironmental cues, resulting in the generation of diverse pluripotent and multipotent states. iPSCs modeled as canonical pluripotent reprogramming are defined by a core transcriptional network, the capacity for tri-lineage differentiation, and a permissive epigenetic landscape. However, growing evidence has revealed other reprogramming trajectories, including direct lineage reprogramming (or transdifferentiation), atypical pluripotent reprogramming, and noncanonical pluripotent reprogramming—exemplified as bacterial protein-mediated multipotency. Here, we summarize emerging insights of canonical and alternative pluripotent reprogramming strategies to propose a more nuanced framework that views pluripotency as a spectrum of molecular and functional states. These unconventional states often exhibit incomplete erasure of somatic identity, altered regulatory networks, and restricted lineage potential, underscoring the plastic and context-dependent nature of pluripotency. This review aims to reconceptualize fundamental perspectives on the acquisition of pluripotency in mammalian fibroblasts based on recent advances. We delineate the criteria for direct lineage reprogramming, atypical pluripotent reprogramming and noncanonical pluripotent reprogramming, explore novel approaches like bacterial ribosome-mediated cell fate conversion. This study integrates these strategies into a unified resource to enhance conceptual clarity and establishes a platform for advancing regenerative medicine.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 6","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.70066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative In Vivo Imaging of ATP Dynamics in Drosophila Using the Ratiometric Biosensor QUEEN 利用比例生物传感器QUEEN对果蝇体内ATP动态进行定量成像。

IF 1.5 4区生物学

Development Growth & Differentiation Pub Date : 2026-08-03 DOI: 10.1111/dgd.70065

Yuhan Luo, Sayaka Sekine, Naoto Kasahara, Hiroyuki Uechi, Takafumi Toyohara, Takaaki Abe, Erina Kuranaga

{"title":"Quantitative In Vivo Imaging of ATP Dynamics in Drosophila Using the Ratiometric Biosensor QUEEN","authors":"Yuhan Luo, Sayaka Sekine, Naoto Kasahara, Hiroyuki Uechi, Takafumi Toyohara, Takaaki Abe, Erina Kuranaga","doi":"10.1111/dgd.70065","DOIUrl":"10.1111/dgd.70065","url":null,"abstract":"Genetically encoded ATP biosensors enable monitoring of cellular energy status, but their application in multicellular organisms remains limited. QUEEN is a ratiometric ATP biosensor consisting of a bacterial ATP-binding protein fused to a circularly permuted fluorescent protein and has been primarily validated in cultured cells. Here, we generated transgenic Drosophila melanogaster lines expressing QUEEN-7μ, enabling tissue-specific expression through the GAL4/UAS system. We characterized its performance in motor neurons and muscles. QUEEN-7μ responses were validated using pharmacological and genetic perturbations of mitochondrial function. Mitochondrial inhibition decreased the QUEEN-7μ ratio, consistent with reduced ATP levels, whereas acute treatment with mitochonic acid-5 (MA-5), a small molecule that enhances mitochondrial ATP synthesis, increased the QUEEN-7μ ratio, with a larger effect at higher concentration. Consistently, genetic manipulations, including the mitochondrial Complex I knockdown and Mitofilin/MIC60 overexpression, produced corresponding decreases and increases in the QUEEN-7μ ratio. These results establish QUEEN-7μ as a reliable ratiometric ATP reporter for quantitative in vivo analysis in Drosophila. This system provides a versatile platform for investigating energy metabolism and mitochondrial function in Drosophila.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 6","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13433815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148671213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AID-Mediated Protein Knockdown Reveals the Requirement of NANOS2 in Prenatal Gonocytes for Establishing Functional Spermatogonial Stem Cells 艾滋病介导的蛋白敲低揭示了产前性腺细胞中NANOS2对建立功能性精原干细胞的需求。

IF 1.5 4区生物学

Development Growth & Differentiation Pub Date : 2026-08-02 DOI: 10.1111/dgd.70064

Yumiko Saga, Quan Wu

{"title":"AID-Mediated Protein Knockdown Reveals the Requirement of NANOS2 in Prenatal Gonocytes for Establishing Functional Spermatogonial Stem Cells","authors":"Yumiko Saga, Quan Wu","doi":"10.1111/dgd.70064","DOIUrl":"10.1111/dgd.70064","url":null,"abstract":"The RNA-binding protein NANOS2 plays a crucial role in male gonocyte development and the maintenance of spermatogonial stem cells. In the absence of the Nanos2 gene (Nanos2-KO), germ cells fail to enter G0 arrest and initiate the male differentiation program (including DNA methylation and the piRNA pathway), ultimately undergoing apoptosis before birth. Nanos2 transcription begins at embryonic day 12.5 (E12.5) and terminates at E15.5. However, as the NANOS2 protein continues to be stably expressed beyond E15.5, it is important to elucidate the function of NANOS2 during this post-E15.5 period in germ cell fate determination. To address the functional significance of sustained NANOS2 protein expression, we employed an auxin-inducible degron (AID2) system to achieve rapid degradation of NANOS2 after E15.5. Within 24 h of 5-Ph-IAA administration, NANOS2 protein was efficiently depleted. As a result, germ cells resumed the cell cycle, exhibited aberrant gene expression patterns similar to Nanos2-KO gonocytes, and underwent apoptosis if NANOS2 depletion occurred at E15.5 or E16.5. Although some surviving cells expressed undifferentiated spermatogonial markers PLZF and GFRA1 after birth, and entered meiotic division, most germ cells disappeared before completing meiosis. These findings reveal that sustained NANOS2 protein expression during the embryonic stage is essential for establishing functional spermatogonial stem cells, highlighting a previously unrecognized regulatory mechanism in male germ cell development.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 6","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13429811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148665025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Replicative Neuromast Regeneration in Response to Injury in Pleurodeles waltl 胸膜壁损伤后神经肥大再生的研究。

IF 1.5 4区生物学

Development Growth & Differentiation Pub Date : 2026-07-14 DOI: 10.1111/dgd.70063

Shinichi Hayashi, Ryohei Seki-Omura, Yuki Sato, Souichi Oe, Taro Koike, Yousuke Nakano, Hikaru Iwashita, Yukie Hirahara, Masaaki Kitada

{"title":"Replicative Neuromast Regeneration in Response to Injury in Pleurodeles waltl","authors":"Shinichi Hayashi, Ryohei Seki-Omura, Yuki Sato, Souichi Oe, Taro Koike, Yousuke Nakano, Hikaru Iwashita, Yukie Hirahara, Masaaki Kitada","doi":"10.1111/dgd.70063","DOIUrl":"10.1111/dgd.70063","url":null,"abstract":"<div>\u0000 \u0000 The lateral line is a mechanosensory organ found in cyclostomes, teleosts, and amphibians. Hair cells within its neuromasts have kinocilia and stereocilia that sense water flow, and the lateral line nerve transduces this sensory input. Hair cells in the lateral line are analogous to those in the mammalian inner ear. Lateral line development and regeneration have been studied extensively in zebrafish. However, lateral line formation in other species remains poorly understood. The Iberian ribbed newt (Pleurodeles waltl) is a versatile model for regeneration with a short period of sexual maturation and aseasonal/prolific ovulation. Here, we investigated the features, regeneration processes, and additional neuromast formation in the posterior lateral line of Iberian ribbed newts using Brainbow reporters. The lateral line in the Iberian ribbed newts shares common features with other species. Laser ablation of hair cells or transection of the posterior lateral line nerve induced additional neuromast formation, to which epidermal cells contributed at least in part. We refer to it as “replicative neuromast regeneration” to allow distinction from several modes of neuromast formation in normal development. Based on these results, we propose that in response to injury, the posterior lateral line nerve induces epidermal cells to contribute to newly formed neuromasts in a replicative manner. Our results provide insights into lateral line morphogenesis.\u0000 </div>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 6","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148450356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sbno1 and Usp8 Cooperate to Enhance Notch Signaling in Regulating Neural Stem Cells sno1和Usp8协同增强Notch信号在神经干细胞调控中的作用

IF 1.5 4区生物学

Development Growth & Differentiation Pub Date : 2026-06-20 DOI: 10.1111/dgd.70062

Oyunchimeg Naranbaatar, Dai Ihara, Hidenori Tabata, Sunjidmaa Zolzaya, Akio Shimizu, Takako Kikkawa, Masahiko Hibi, Hisakazu Ogita, Yu Katsuyama

{"title":"Sbno1 and Usp8 Cooperate to Enhance Notch Signaling in Regulating Neural Stem Cells","authors":"Oyunchimeg Naranbaatar, Dai Ihara, Hidenori Tabata, Sunjidmaa Zolzaya, Akio Shimizu, Takako Kikkawa, Masahiko Hibi, Hisakazu Ogita, Yu Katsuyama","doi":"10.1111/dgd.70062","DOIUrl":"https://doi.org/10.1111/dgd.70062","url":null,"abstract":"<div>\u0000 \u0000 The Notch signaling pathway is essential for the self-renewal, differentiation, and maintenance of neural stem cells (NSCs). While genetic studies in Drosophila have implicated Strawberry Notch in Notch signaling, the role of its vertebrate homologue, Sbno1, in mammalian NSCs remains largely unexplored. Here we show that Sbno1 interacts with Rbpj, the central transcriptional mediator of Notch, as well as with the deubiquitinating enzyme Usp8. Promoter analyses revealed that Sbno1 and Usp8 synergistically enhance expression of Hes5, a well-known Notch target in NSCs. Using in utero electroporation to overexpress or knock out Usp8 in embryonic cortical NSCs, we demonstrate that Usp8 is essential for NSC maintenance whereas loss of Sbno1 leads to NSC depletion and premature neuronal differentiation. Mechanistically, our results suggest that Sbno1 facilitates Usp8-mediated stabilization of the Notch intracellular domain and resolves R-loops at the Hes5 promoter, thereby promoting target gene expression. Together, these findings identify Sbno1 and Usp8 as critical, previously unrecognized regulators of Notch signaling in NSCs and provide the first in vivo evidence of their physiological roles in mammalian cortical development.\u0000 </div>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 4-5","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148282537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0