Development Growth & Differentiation最新文献

Regeneration of Lumbriculus variegatus requires post-amputation production of reactive oxygen species. 畸形蚓的再生需要在截肢后产生活性氧。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2025-01-21 DOI: 10.1111/dgd.12961

Freya R Beinart, Kathy Gillen

引用次数: 0

"Fly to New World": Meeting report of the 16th Japanese Drosophila research conference (JDRC16). “飞向新世界”：第16届日本果蝇研究大会（JDRC16）会议报告。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2025-01-18 DOI: 10.1111/dgd.12959

Haruka Yoshizawa, Erina Kuranaga

{"title":"\"Fly to New World\": Meeting report of the 16th Japanese Drosophila research conference (JDRC16).","authors":"Haruka Yoshizawa, Erina Kuranaga","doi":"10.1111/dgd.12959","DOIUrl":"https://doi.org/10.1111/dgd.12959","url":null,"abstract":"The 16th Japanese Drosophila Research Conference (JDRC16) was held at the Sendai International Center from September 17 to 19 2024. It had been 2 years since the last JDRC15 held in Nagoya. The conference brought together 231 researchers, including 22 researchers from overseas, creating a vibrant and diverse platform for scientific exchange. Prof. Shigeo Hayashi of RIKEN BDR delivered a keynote lecture, and his groundbreaking ideas and research captivated the audience. Over the 3 days, the conference featured 53 oral presentations across 11 sessions and 2 special sessions, as well as 128 poster presentations, all of which fostered stimulating discussions and the exchange of innovative ideas. The reception provided an additional opportunity for researchers to engage in meaningful dialogue while enjoying Sendai's renowned specialties. Held under clear autumn skies in a great nature along the river, this conference painted a beautiful contrast to the heated discussions in the venue. Consequently, this conference fully contributed to the mission proffered by Prof. Hayashi, \"Fly to New World,\" expanding the insights gained from flies into new and unexplored scientific areas.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transmembrane protein TMEM196 controls cell proliferation and determines the floor plate cell lineage. 跨膜蛋白TMEM196控制细胞增殖并决定底板细胞谱系。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2025-01-18 DOI: 10.1111/dgd.12960

Yumi Matsumoto, Seiichi Tamaru, Xing Chen, Takuma Shinozuka, Yuichi Sakumura, Noriaki Sasai

{"title":"The transmembrane protein TMEM196 controls cell proliferation and determines the floor plate cell lineage.","authors":"Yumi Matsumoto, Seiichi Tamaru, Xing Chen, Takuma Shinozuka, Yuichi Sakumura, Noriaki Sasai","doi":"10.1111/dgd.12960","DOIUrl":"https://doi.org/10.1111/dgd.12960","url":null,"abstract":"The neural tube, the embryonic precursor to the vertebrate central nervous system, comprises distinct progenitor and neuronal domains, each with specific proliferation programs. In this study, we identified TMEM196, a novel transmembrane protein that plays a crucial role in regulating cell proliferation in the floor plate in chick embryos. TMEM196 is expressed in the floor plate, and its overexpression leads to reduced cell proliferation without affecting the pattern formation of the neural tube. We also established the floor plate differentiation protocol of the mouse embryonic stem cells, and analyzed the function of TMEM196 with this system. Mutating the Tmem196 gene does not alter cell division and overall differentiation remains unchanged within the neural cells. However, TMEM196 inhibits Wnt signaling, and Tmem196 mutant cells exhibit aberrant paraxial mesoderm differentiation, suggesting that TMEM196 selects the floor plate cell fate at the binary decision of the neuromesodermal cells. These findings highlight TMEM196 as a key regulator of both cell proliferation and floor plate determination, contributing to proper regionalization during embryogenesis.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early development of the calcified exoskeleton of the polyplacophoran mollusk, with insight into the evolutionary history of shell plates and spicules. 多placophoran软体动物钙化外骨骼的早期发育，并深入了解壳板和针状体的进化史。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2025-01-10 DOI: 10.1111/dgd.12956

Hiroki Yoshikawa, Yoshiaki Morino, Hiroshi Wada

引用次数: 0

Sonic Hedgehog signaling regulates the optimal differentiation pace from early-stage mesoderm to cardiogenic mesoderm in mice. Sonic Hedgehog信号调控小鼠早期中胚层向心源性中胚层的最佳分化速度。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2025-01-09 DOI: 10.1111/dgd.12955

Satoshi Inoue, Moe Nosetani, Yoshiro Nakajima, Shinichiro Sakaki, Hiroki Kato, Rie Saba, Naoki Takeshita, Kosuke Nishikawa, Atsuko Ueyama, Kazuhiko Matsuo, Masaki Shigeta, Daisuke Kobayashi, Tomoko Iehara, Kenta Yashiro

{"title":"Sonic Hedgehog signaling regulates the optimal differentiation pace from early-stage mesoderm to cardiogenic mesoderm in mice.","authors":"Satoshi Inoue, Moe Nosetani, Yoshiro Nakajima, Shinichiro Sakaki, Hiroki Kato, Rie Saba, Naoki Takeshita, Kosuke Nishikawa, Atsuko Ueyama, Kazuhiko Matsuo, Masaki Shigeta, Daisuke Kobayashi, Tomoko Iehara, Kenta Yashiro","doi":"10.1111/dgd.12955","DOIUrl":"https://doi.org/10.1111/dgd.12955","url":null,"abstract":"Sonic Hedgehog (Shh), encoding an extracellular signaling molecule, is vital for heart development. Shh null mutants show congenital heart disease due to left-right asymmetry defects stemming from functional anomaly in the midline structure in mice. Shh signaling is also known to affect cardiomyocyte differentiation, endocardium development, and heart morphogenesis, particularly in second heart field (SHF) cardiac progenitor cells that contribute to the right ventricle, outflow tract, and parts of the atrium. Despite extensive studies, our understanding remains incomplete. Notably, Shh signaling is suggested to promote cardiac differentiation, while paradoxically preventing premature differentiation of SHF progenitors. In this study, we elucidate the role of Shh signaling in the earliest phase of cardiac differentiation. Our meta-analysis of single-cell RNA sequencing suggests that cardiogenic nascent mesoderm cells expressing the bHLH transcription factor Mesp1 interact with axial mesoderm via Hh signaling. Activation of Hh signaling using a Smoothened agonist delayed or suppressed the differentiation of primitive streak cells expressing T-box transcription factor T to Mesp1+ nascent mesoderm cells both in vitro and ex vivo. Conversely, inhibition of Hh signaling by cyclopamine facilitated cardiac differentiation. The reduction of Eomes, an inducer of Mesp1, by Hh signaling appears to be the underlying mechanism of this phenomenon. Our data suggest that SHH secreted from axial mesoderm inhibits premature differentiation of T+ cells to Mesp1+ nascent mesoderm cells, thereby regulating the pace of cardiac differentiation. These findings enhance our comprehension of Shh signaling in cardiac development, underscoring its crucial role in early cardiac differentiation.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142958326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparative approach to the microstructure in the carpus and tarsus in anurans. anurans腕骨和跗骨微观结构的比较研究。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2025-01-08 DOI: 10.1111/dgd.12957

Marissa Fabrezi, Julio César Cruz

{"title":"A comparative approach to the microstructure in the carpus and tarsus in anurans.","authors":"Marissa Fabrezi, Julio César Cruz","doi":"10.1111/dgd.12957","DOIUrl":"https://doi.org/10.1111/dgd.12957","url":null,"abstract":"Despite the significant literature about morphological features of limb skeletons involved in tetrapod limb evolution, some questions about carpal and tarsal elements remain. In anurans, the ecomorphological and biomechanical approaches studied long hind limbs (to jump) and forelimbs (to land) and emphasized the role of the long bones in locomotion but disregarded what happens with the nodular elements of the carpus and tarsus. Here, we present a comparative study of nodular elements of the carpus and tarsus in anurans based on whole-mount specimens stained with Alcian Blue (cartilage) and Alizarin Red S (bone and calcified cartilage). The sample comprises 113 species belonging to 33 anuran families and postmetamorphic series in selected species. Further, we analyze the histology of the carpus and tarsus in individuals of nine species. In most anurans, the carpal and tarsal elements are cartilaginous in adult stages. The cartilaginous matrix may present different degrees of calcification. Few taxa present truly ossified carpals and tarsals with marrow cavity, blood cells, and hematopoietic cells. Interpretation of the interspecific variation in the carpus and tarsus skeletons on the most recent anuran phylogeny suggests that the delayed ossification of carpals and tarsals has evolved in derived lineages (e.g. Pelobatoidea and Neobatrachia).","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142958284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Cdk1 gene disruption on cell cycle progression in newt cells. Cdk1基因破坏对蝾螈细胞周期进程的影响。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2025-01-08 DOI: 10.1111/dgd.12958

Yuta Nakao, Kazuko Okamoto, Ichiro Tazawa, Tatsuro Nishijima, Nobuaki Furuno, Tetsushi Sakuma, Takashi Yamamoto, Takashi Takeuchi, Toshinori Hayashi

{"title":"Effect of Cdk1 gene disruption on cell cycle progression in newt cells.","authors":"Yuta Nakao, Kazuko Okamoto, Ichiro Tazawa, Tatsuro Nishijima, Nobuaki Furuno, Tetsushi Sakuma, Takashi Yamamoto, Takashi Takeuchi, Toshinori Hayashi","doi":"10.1111/dgd.12958","DOIUrl":"https://doi.org/10.1111/dgd.12958","url":null,"abstract":"Cyclin-dependent kinases (CDKs) are key regulators of cell cycle progression, in conjunction with cyclins. The cyclin-CDK system is highly conserved among eukaryotes, and CDK1 is considered essential for progression through the M phase. However, the extent to which cell cycle progression depends on CDK1 varies between cell types. Therefore, a range of cell types must be analyzed to comprehensively elucidate the role of CDK1. Cdk1-knockout mice exhibit lethality at an early developmental stage, specifically before the differentiation of various cell types. The aim of the present study was to characterize the effects of CDK1 deficiency in amphibian newts. Cdk1 was disrupted by injecting fertilized newt eggs with CRISPR/Cas9, and the resulting effects on embryonic development and cell proliferation were then evaluated. In both wild-type and Cdk1-crispant newt embryos, CDK1 protein was either stored in the egg until late embryogenesis or potentially derived from maternal mRNA, which may also be stored during this period. The embryos survived to the hatching stage, during which the cells responsible for forming the basic organs differentiated. To further characterize the long-term effects of Cdk1 knockout, parabiosis experiments were conducted using wild-type embryos and Cdk1 crispants. The results suggested that an endocycle occurred in the crispant larvae, as evidenced by increases in the size of several types of cells. It is anticipated that studies using newts will provide further insights into the role of Cdk1 in regulating the cell cycle.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142958322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meeting report about self-organization in biology: Freiburg Spemann-Mangold Centennial Symposium. 生物学中自组织的会议报告：弗莱堡Spemann-Mangold百年学术研讨会。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2025-01-08 DOI: 10.1111/dgd.12954

Satoshi Kuwana, Yuuri Yasuoka

{"title":"Meeting report about self-organization in biology: Freiburg Spemann-Mangold Centennial Symposium.","authors":"Satoshi Kuwana, Yuuri Yasuoka","doi":"10.1111/dgd.12954","DOIUrl":"https://doi.org/10.1111/dgd.12954","url":null,"abstract":"From September 16 to 19, 2024, an international symposium to celebrate the centennial of the discovery of the gastrula organizer by Hans Spemann and Hilde Mangold, was held at the University of Freiburg, Germany, where they studied embryology. There were 41 plenary lectures, 11 short talks, and 182 poster presentations, with more than 300 participants from 23 countries. The symposium covered research topics broadly related to developmental, cell, genome, and evolutionary biology, mainly focused on early animal development. In addition to in vivo studies on topics such as gastrulation, embryonic patterning, cell polarity, and morphogenesis, recent studies using gastruloids and organoids, which recapitulate embryogenesis and organogenesis in in vitro cell culture, were also presented at this symposium, entitled Self-Organization in Biology. Most of the reported studies used vertebrate models such as mice, frogs, and zebrafish; however, evolutionary studies involving invertebrate and plant models were also presented. Presentations employing traditional methods such as cell transplantation and phenotype screening, and state-of-the-art technologies such as single-cell omics, high-resolution imaging, and computational analysis showed that experimental embryology has a long history, to which studies of the organizer have contributed significantly. Here we discuss memorable aspects of the symposium in the hope that this report will encourage young scientists to actively participate in face-to-face international conferences.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142958324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel function of Hox13 in regulating outgrowth of the newt hindlimb bud through interaction with Fgf10 and Tbx4. Hox13通过与Fgf10和Tbx4相互作用调控蝾螈后肢芽生长的新功能。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2025-01-01 Epub Date: 2024-12-26 DOI: 10.1111/dgd.12952

Sayo Tozawa, Haruka Matsubara, Fumina Minamitani, Yasuhiro Kamei, Misako Saida, Momoko Asao, Ken-Ichi T Suzuki, Masatoshi Matsunami, Shuji Shigenobu, Toshinori Hayashi, Gembu Abe, Takashi Takeuchi

{"title":"Novel function of Hox13 in regulating outgrowth of the newt hindlimb bud through interaction with Fgf10 and Tbx4.","authors":"Sayo Tozawa, Haruka Matsubara, Fumina Minamitani, Yasuhiro Kamei, Misako Saida, Momoko Asao, Ken-Ichi T Suzuki, Masatoshi Matsunami, Shuji Shigenobu, Toshinori Hayashi, Gembu Abe, Takashi Takeuchi","doi":"10.1111/dgd.12952","DOIUrl":"10.1111/dgd.12952","url":null,"abstract":"5'Hox genes regulate pattern formation along the axes of the limb. Previously, we showed that Hoxa13/Hoxd13 double-mutant newts lacked all digits of the forelimbs during development and regeneration, showing that newt Hox13 is necessary for digit formation in development and regeneration. In addition, we found another unique phenotype. Some of the Hox13 crispant newts showed hindlimb defects, in which whole or almost whole hindlimbs were lost, suggesting a novel function of Hox13 in limb development. Using germline mutants, we showed that mutation in Hox13 led to hindlimb defects. The limb buds of Hox13 crispants formed, however, did not show outgrowth. Expression of Fgf10 and Tbx4, which are involved in limb outgrowth, decreased in the hindlimb buds of Hox13 crispants. In addition, hindlimb defects were observed in both Fgf10 and Tbx4 crispant newts. Finally, Fgf10 and Tbx4 interacted with Hox13 genetically. Our results revealed a novel function of Hox13 in regulating the outgrowth of the newt hindlimb bud through interaction with Fgf10 and Tbx4.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":" ","pages":"10-22"},"PeriodicalIF":1.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142900061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meeting report: Society for Developmental Biology 83rd annual meeting. 会议报告：发育生物学学会第83届年会。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2025-01-01 Epub Date: 2024-12-03 DOI: 10.1111/dgd.12950

Shunsuke Yaguchi

引用次数: 0