Development Growth & Differentiation最新文献

Measuring intraventricular pressure in developing mouse embryos: Uncovering a repetitive mechanical cue for brain development. 测量发育中的小鼠胚胎的脑室内压力：揭示大脑发育的重复机械线索。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2025-05-13 DOI: 10.1111/dgd.70010

Mami Akaike, Jun Hatakeyama, Yuta Nakashima, Kenji Shimamura

{"title":"Measuring intraventricular pressure in developing mouse embryos: Uncovering a repetitive mechanical cue for brain development.","authors":"Mami Akaike, Jun Hatakeyama, Yuta Nakashima, Kenji Shimamura","doi":"10.1111/dgd.70010","DOIUrl":"https://doi.org/10.1111/dgd.70010","url":null,"abstract":"In living organisms, including humans, the developmental processes that construct their morphology from a single fertilized egg are influenced not only by genetic regulation but also by various external factors. One such factor is mechanical stimulation. Although mechanical forces are suggested to contribute to brain formation during development, quantitative information on intraventricular pressure during neurogenesis remains limited. We developed a high time-resolution system efficiently using a piezoresistive sensor to measure brain intraventricular pressure in mouse embryos from E12.5 to E16.5 (embryonic stages in days). Ex utero measurements revealed intraventricular pressure increasing from 53.76 ± 4.16 Pa at E12.5 to 158.10 ± 19.94 Pa by E16.5. In utero analyses uncovered striking periodicity in sync with uterine contractions, reaching up to 1430 ± 195.2 Pa at E12.5, indicating dynamic mechanical stimuli beyond ex utero observations. Additionally, perforation experiments at E9.0-E15.5 showed rapid neuroepithelial thickening and apical surface contraction upon pressure release, indicative of a tensile effect by the positive intraventricular pressure. This effect diminished after E15.5, implying that tension wanes or the neuroepithelium becomes more robust. These results highlight the dynamic nature of embryonic intraventricular pressure, governed by internal fluid production and uterine forces, and emphasize the importance of mechanical cues in neuroepithelial architecture. Our findings provide a steppingstone to clarify how mechanical forces integrate with genetic and molecular processes to shape normal brain development and may render new perspectives on brain evolution.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144052031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The long-term survival of enteroendocrine cells depends on their subtype and is linked to peripheral sensory innervation. 肠内分泌细胞的长期存活取决于其亚型，并与外周感觉神经支配有关。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2025-05-07 DOI: 10.1111/dgd.70009

Salsabila Luthfi Sesotyosari, Masato Kinoshita, Mukhamad Sunardi, Mo Lihan, Akimasa Orii, Takaya Abe, Hiroshi Kiyonari, Tatsuya Nakai, Toshihiro Uesaka, Yuzo Kodama, Hideki Enomoto

{"title":"The long-term survival of enteroendocrine cells depends on their subtype and is linked to peripheral sensory innervation.","authors":"Salsabila Luthfi Sesotyosari, Masato Kinoshita, Mukhamad Sunardi, Mo Lihan, Akimasa Orii, Takaya Abe, Hiroshi Kiyonari, Tatsuya Nakai, Toshihiro Uesaka, Yuzo Kodama, Hideki Enomoto","doi":"10.1111/dgd.70009","DOIUrl":"https://doi.org/10.1111/dgd.70009","url":null,"abstract":"Enteroendocrine cells (EECs) are sensory epithelial cells that sense the gut luminal environment and convey sensory information to the brain via the visceral afferent pathway. Although EECs are a part of gut epithelial cells, which generally undergo rapid turnover, some EECs have been reported to be long-lived. EECs consist of multiple subtypes, each of which displays distinct hormone production and distribution patterns. It remains unknown whether a long lifespan is a characteristic shared by all EEC subtypes. To address this issue, we conducted genetic pulse labeling of three EEC subtypes expressing serotonin (5-HT), peptide YY (PYY), and gastric inhibitory polypeptide (GIP) in mice and tracked their survival. In the proximal small intestine, all labeled GIP+ EECs disappeared completely within 5 days, whereas some PYY+ EECs survived for more than 7 days. In the proximal colon, some labeled 5-HT+ EECs lived for more than 28 days, whereas no PYY+ cells survived beyond 14 days. These long-lived 5-HT+ EECs were almost exclusively found in the upper half of the crypt in the mucosal fold, where visceral sensory fibers were enriched. This study reveals subtype- and region-dependent survival of EECs and suggests that EEC-nerve communication may underlie the long lifespan of certain EECs.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144059600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Waves of innovation and collaboration: Zebrafish research converges in Kyoto. 创新与合作浪潮：斑马鱼研究汇聚京都。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2025-05-05 DOI: 10.1111/dgd.70008

Takuya Kaneko

引用次数: 0

Efficient CRISPR/Cas9-mediated knockin of reporter genes in rats at ROSA26 by pronuclear microinjection. 原核微注射CRISPR/ cas9介导的ROSA26大鼠报告基因敲入

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2025-04-23 DOI: 10.1111/dgd.70007

Takaya Abe, Ken-Ichi Inoue, Hiroshi Kiyonari

引用次数: 0

Integrative approaches in Xenopus-based research: A report from the 1st Asian Xenopus Conference. 基于章鱼研究的综合方法：第一届亚洲爪蟾大会报告。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2025-04-03 DOI: 10.1111/dgd.70006

Dong Gil Jang, Hyo Jung Sim, Keun Yeong Kwon

{"title":"Integrative approaches in Xenopus-based research: A report from the 1st Asian Xenopus Conference.","authors":"Dong Gil Jang, Hyo Jung Sim, Keun Yeong Kwon","doi":"10.1111/dgd.70006","DOIUrl":"https://doi.org/10.1111/dgd.70006","url":null,"abstract":"The first Asian community conference on \"Scientific research using Xenopus as an animal model system\" was held in Osaka, Japan, from November 24 to 26, 2024. This event was organized by the Korean Society for Xenopus Development of KSMCB (Korean Society for the Molecular and Cellular Biology), Xenopus Community in Japan (XCIJ), KNU G-LAMP Project, National BioResource Project-Clawed frogs/Newts (NBRP), and Osaka University. The conference covered various research topics in biological sciences using Xenopus. The ultimate goal of this conference was to promote collaboration and encourage a new and sustainable relationship among researchers in the Asian region who utilize Xenopus in their studies by sharing scientific findings. The conference not only shared research findings from various biological fields in the Asian region but also provided opportunities to invite global experts in the Xenopus research for \"Keynote Lectures\" and rising researchers who use other animal models for the \"Friends of Xenopus\" session to foster interdisciplinary exchange. In addition, organizers planned a luncheon seminar to help trainee students learn how to write a scientific paper and how not to.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and developmental bases for mammalian neocortical evolution 哺乳动物新皮层进化的遗传和发育基础。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2025-03-29 DOI: 10.1111/dgd.70003

Tadashi Nomura, Chiaki Ohtaka-Maruyama

引用次数: 0

Collagen fiber and cellular dynamics of axolotl skin with aging. 老化时蝾螈皮肤的胶原纤维与细胞动力学。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2025-03-24 DOI: 10.1111/dgd.70005

Chisaki Shima, Ayaka Ohashi, Saya Furukawa, Sakiya Yamamoto, Rena Kashimoto, Akira Satoh

{"title":"Collagen fiber and cellular dynamics of axolotl skin with aging.","authors":"Chisaki Shima, Ayaka Ohashi, Saya Furukawa, Sakiya Yamamoto, Rena Kashimoto, Akira Satoh","doi":"10.1111/dgd.70005","DOIUrl":"https://doi.org/10.1111/dgd.70005","url":null,"abstract":"As skin ages, its structure and function undergo significant transformations driven by complex cellular and molecular processes. In this study, we explore these changes using the axolotl, an amphibian model known for its transparent skin, allowing detailed observation of both epidermal and dermal layers. We found that axolotl skin, composed of an epidermis and a collagen-rich dermis with three distinct layers (stratum baladachinum, spongiosum, and compactum), shows clear age-related alterations. These changes include reduced fibroblast numbers, altered lattice-patterned cell morphology, disruption of the lattice patterned collagen fiber pattern, thickening the stratum spongiosum, and thinning of the stratum compactum. Notably, fibroblasts, which play a crucial role in collagen braiding, displayed diminished functionality in older axolotls. This study highlights how aging affects both the structural integrity of dermal collagen and cellular dynamics. Given the similarity between axolotl and mammalian skin, these findings may provide valuable insights into the mechanisms of skin aging and potential avenues for anti-aging therapies. This research offers a foundation for future studies aimed at understanding skin aging and regeneration.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment and characterization of adap1-deficient zebrafish adap1缺陷斑马鱼的建立和特征描述

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2025-03-15 DOI: 10.1111/dgd.70004

Atsuo Kawahara, Sakyo Yasojima, Junko Koiwa, Saori Fujimaki, Hiroaki Ito, Mamiko Yamada, Kenjiro Kosaki, Yuhei Nishimura

{"title":"Establishment and characterization of adap1-deficient zebrafish","authors":"Atsuo Kawahara, Sakyo Yasojima, Junko Koiwa, Saori Fujimaki, Hiroaki Ito, Mamiko Yamada, Kenjiro Kosaki, Yuhei Nishimura","doi":"10.1111/dgd.70004","DOIUrl":"10.1111/dgd.70004","url":null,"abstract":"The adap1 (ADP-ribosylation factor GTPase-activating protein [ArfGAP] with dual pleckstrin homology [PH] domains 1) gene is predominantly expressed in the mouse brain and is important in neural differentiation and development. However, the functions of adap1 in morphogenesis, locomotor activity, and behaviors in vertebrates are not fully understood. Whole-mount in situ hybridization (WISH) analysis revealed that adap1 was widely expressed in the zebrafish brain, including the forebrain, midbrain, and hindbrain, during early embryogenesis. To investigate the physiological function of the adap1 gene, we generated zebrafish adap1 mutants harboring frameshift mutations around codon 120 of adap1. The adap1 mutants containing homozygous mutant alleles exhibited no apparent morphological abnormalities at 1 day postfertilization (dpf), and the spontaneous coiling and touch response of the adap1 mutants were comparable to those of the wild-type fish. In addition, the expression of neural genes, such as emx1, mbx, and huC, was comparable between the wild-type fish and the adap1 mutants at 1 dpf. The adap1 mutants grew to adulthood without exhibiting any apparent swimming defects. The adult adap1 mutants spent more time than the wild type in the center region of the open field test. In the social behavior test, zebrafish containing the adap1 mutant alleles spent more time than the wild type in the regions near the chambers where novel conspecifics swam. These results imply the involvement of the adap1 gene in regulating approach behavior to visual cues from conspecifics.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"67 3","pages":"165-173"},"PeriodicalIF":1.7,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.70004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transforming growth factor-β-mediated regulation of atoh1-expressing neural progenitors is involved in the generation of cerebellar granule cells in larval and adult zebrafish 转化生长因子-β介导的表达atoh1的神经祖细胞的调控参与了幼体和成年斑马鱼小脑颗粒细胞的产生。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2025-02-27 DOI: 10.1111/dgd.70002

Jui Chun Wang, Takashi Shimizu, Masahiko Hibi

{"title":"Transforming growth factor-β-mediated regulation of atoh1-expressing neural progenitors is involved in the generation of cerebellar granule cells in larval and adult zebrafish","authors":"Jui Chun Wang, Takashi Shimizu, Masahiko Hibi","doi":"10.1111/dgd.70002","DOIUrl":"10.1111/dgd.70002","url":null,"abstract":"Granule cells in the cerebellum are the most numerous neurons in the vertebrate brain. They are derived from neural progenitor cells that express the proneural gene atoh1 (atoh1a, b, c in zebrafish) during early neurogenesis. In zebrafish, unlike in mammals, granule cells are continuously produced throughout life, from the larval stage to adulthood. Additionally, granule cells regenerate and replace damaged areas following injury in the adult cerebellum. However, the mechanisms underlying granule cell generation and their role in adult cerebellar regeneration remain largely unclear. In this study, using lineage tracing with the inducible DNA recombinase CreERT2, we found that granule cells differentiated from atoh1c-expressing neural progenitor cells and migrated to their appropriate locations in the adult stage, similar to the processes observed during early embryogenesis. Granule cells that differentiated from atoh1c-expressing neural progenitor cells in adulthood also contributed to cerebellar regeneration. Furthermore, inhibition of transforming growth factor-β (TGF-β) signaling, either via chemical inhibitors or CRISPR/Cas9, suppressed atoh1a/c expression and reduced granule cell numbers in larvae. Chemical inhibition of TGF-β signaling also suppressed neural progenitor cell proliferation, atoh1c expression, and granule cell neurogenesis in the adult cerebellum. These findings demonstrate that TGF-β signaling is essential for granule cell production from progenitor cells throughout the lifespan of zebrafish.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"67 3","pages":"149-164"},"PeriodicalIF":1.7,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.70002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143517256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The property of larval cells of the scleractinian coral, Acropora tenuis, deduced from in vitro cultured cells 从体外培养的细胞推导出硬核珊瑚（Acropora tenuis）幼虫细胞的特性。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2025-02-21 DOI: 10.1111/dgd.70000

Kaz Kawamura, Satoko Sekida, Koki Nishitsuji, Noriyuki Satoh

{"title":"The property of larval cells of the scleractinian coral, Acropora tenuis, deduced from in vitro cultured cells","authors":"Kaz Kawamura, Satoko Sekida, Koki Nishitsuji, Noriyuki Satoh","doi":"10.1111/dgd.70000","DOIUrl":"10.1111/dgd.70000","url":null,"abstract":"In previous studies, we have established approximately 15 cultured cell-lines derived from planula larvae of Acropora tenuis. Based on their morphology and behavior, these cells were classified into three types, flattened amorphous cells (FAmCs), vacuolated adherent cells (VAdCs), and small smooth cells (SSmCs). FAmCs include fibroblast-like cells and spherical, brilliant brown cells (BBrCs), which are transformable to each other. To examine the larval origin of the three cell types, we raised antibodies: anti-AtMLRP2 that appears to recognize FAmC, anti-AtAHNAK for BBrC, anti-AtSOMP5 and anti-AtEndoG for SSmC, and anti-AtGal and anti-AtFat4 for VAdC, respectively. Anti-AtMLRP2 antibody stained in vivo stomodeum and neuroblast-like cells embedded in larval ectoderm around the aboral pole. Anti-AtAHNAK antibody stained neuron-like and neuroblast-like cells, both of which were also stained with neuron-specific tubulin β-3 antibody. These results suggest that in vitro BBrCs and in vivo neuroblast-like cells share neuronal properties in common. Two antibodies for SSmCs, anti-AtSOMP5 and anti-AtEndoG, stained larval ectoderm cells, suggesting that SSmCs have larval ectoderm properties. Two antibodies for VAdCs, anti-AtGal and anti-AtFat4, stained larval endoderm cells, suggesting that VAdCs have larval endoderm properties. Therefore, the in vitro cell lines appear to retain properties of the stomodeum, neuroblast, ectoderm, or endoderm. Each of them may be used in future investigations to reveal cellular and molecular properties of cell types of coral larvae, such as the potential for symbiosis.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"67 3","pages":"119-135"},"PeriodicalIF":1.7,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.70000","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143469952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0