Development Growth & Differentiation最新文献

Quantitative in toto live imaging analysis of apical nuclear migration in the mouse telencephalic neuroepithelium. 小鼠端脑神经上皮细胞顶端核迁移的定量全图活体成像分析。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2024-11-26 DOI: 10.1111/dgd.12949

Tsukasa Shimamura, Takaki Miyata

{"title":"Quantitative in toto live imaging analysis of apical nuclear migration in the mouse telencephalic neuroepithelium.","authors":"Tsukasa Shimamura, Takaki Miyata","doi":"10.1111/dgd.12949","DOIUrl":"https://doi.org/10.1111/dgd.12949","url":null,"abstract":"In the embryonic neuroepithelium (NE), neural progenitor cells undergo cell cycle-dependent interkinetic nuclear migration (IKNM) along the apicobasal axis. Extensive IKNM supports increasing cell production rates per unit apical surface, as typically observed in the mammalian telencephalic NE. Apical nucleokinesis during the G2 phase is an essential premitotic event, but its occurrence has not yet been quantitatively analyzed at a large 3D-scale with sufficient spatiotemporal resolution. Here, we comprehensively analyzed apically migrating nuclei/somata in reference to their surroundings from embryonic day (E)11 to E13 in the mouse telencephalon. The velocity of apical nucleokinesis decreased, with more frequent nuclear pausing occurring at E12 and E13, whereas the nuclear density in the middle NE zone (20-40-μm deep) increased. This result, together with the results of Shh-mediated overproliferation experiments in which the nuclear density was increased in vivo at E11, suggests that apical nucleokinesis is physically influenced by the surrounding nuclei. Mean square displacement analysis for nuclei being passed by the apically migrating nuclei via horizontal sectioning in toto-recorded movies revealed that the \"tissue fluidity\" or physical permissiveness of the NE to apical nucleokinesis gradually decreased (E11 > E12 > E13). To further investigate the spatial relationship between preexisting mitoses and subsequent premitotic apical nucleokinesis, the horizontal distribution of mitoses was cumulatively (~3 hr) analyzed under in toto monitoring. The four-dimensional cumulative apical mitoses presented a \"random\", not \"clustered\" or \"regular\", distribution pattern throughout the period examined. These methodologies provide a basis for future comparative studies of interspecies differences.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Labeling and sorting of avian primordial germ cells utilizing Lycopersicon Esculentum lectin. 利用Lycopersicon Esculentum凝集素对禽类原始生殖细胞进行标记和分类。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2024-11-09 DOI: 10.1111/dgd.12948

Hiroko Iikawa, Aika Nishina, Mizuki Morita, Yuji Atsuta, Yoshiki Hayashi, Daisuke Saito

引用次数: 0

Transition from fetal to postnatal state in the heart: Crosstalk between metabolism and regeneration. 心脏从胎儿状态向出生后状态的过渡：新陈代谢与再生之间的相互影响

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2024-10-27 DOI: 10.1111/dgd.12947

Tai Sada, Wataru Kimura

{"title":"Transition from fetal to postnatal state in the heart: Crosstalk between metabolism and regeneration.","authors":"Tai Sada, Wataru Kimura","doi":"10.1111/dgd.12947","DOIUrl":"https://doi.org/10.1111/dgd.12947","url":null,"abstract":"Cardiovascular disease is the leading cause of mortality worldwide. Myocardial injury resulting from ischemia can be fatal because of the limited regenerative capacity of adult myocardium. Mammalian cardiomyocytes rapidly lose their proliferative capacities, with only a small fraction of adult myocardium remaining proliferative, which is insufficient to support post-injury recovery. Recent investigations have revealed that this decline in myocardial proliferative capacity is closely linked to perinatal metabolic shifts. Predominantly glycolytic fetal myocardial metabolism transitions towards mitochondrial fatty acid oxidation postnatally, which not only enables efficient production of ATP but also causes a dramatic reduction in cardiomyocyte proliferative capacity. Extensive research has elucidated the mechanisms behind this metabolic shift, as well as methods to modulate these metabolic pathways. Some of these methods have been successfully applied to enhance metabolic reprogramming and myocardial regeneration. This review discusses recently acquired insights into the interplay between metabolism and myocardial proliferation, emphasizing postnatal metabolic transitions.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142512319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial DNA replication is essential for neurogenesis but not gliogenesis in fetal neural stem cells 线粒体DNA复制对胎儿神经干细胞的神经发生（neurogenesis）至关重要，但对神经胶质细胞的发生（gliogenesis）并不重要。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2024-10-22 DOI: 10.1111/dgd.12946

Meri Walter-Manucharyan, Melanie Martin, Julia Pfützner, Franz Markert, Gerhard Rödel, Andreas Deussen, Andreas Hermann, Alexander Storch

{"title":"Mitochondrial DNA replication is essential for neurogenesis but not gliogenesis in fetal neural stem cells","authors":"Meri Walter-Manucharyan, Melanie Martin, Julia Pfützner, Franz Markert, Gerhard Rödel, Andreas Deussen, Andreas Hermann, Alexander Storch","doi":"10.1111/dgd.12946","DOIUrl":"10.1111/dgd.12946","url":null,"abstract":"Mitochondria are unique organelles that have their own genome (mtDNA) and perform various pivotal functions within a cell. Recently, evidence has highlighted the role of mitochondria in the process of stem cell differentiation, including differentiation of neural stem cells (NSCs). Here we studied the importance of mtDNA function in the early differentiation process of NSCs in two cell culture models: the CGR8-NS cell line that was derived from embryonic stem cells by a lineage selection technique, and primary NSCs that were isolated from embryonic day 14 mouse fetal forebrain. We detected a dramatic increase in mtDNA content upon NSC differentiation to adapt their mtDNA levels to their differentiated state, which was not accompanied by changes in mitochondrial transcription factor A expression. As chemical mtDNA depletion by ethidium bromide failed to generate living ρ° cell lines from both NSC types, we used inhibition of mtDNA polymerase-γ by 2′-3′-dideoxycytidine to reduce mtDNA replication and subsequently cellular mtDNA content. Inhibition of mtDNA replication upon NSC differentiation reduced neurogenesis but not gliogenesis. The mtDNA depletion did not change energy production/consumption or cellular reactive oxygen species (ROS) content in the NSC model used. In conclusion, mtDNA replication is essential for neurogenesis but not gliogenesis in fetal NSCs through as yet unknown mechanisms, which, however, are largely independent of energy/ROS metabolism.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 8","pages":"398-413"},"PeriodicalIF":1.7,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12946","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142512320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lineage tracing of Shh+ floor plate cells and dynamics of dorsal–ventral gene expression in the regenerating axolotl spinal cord 再生腋龙脊髓中Shh+底板细胞的系谱追踪和背腹基因表达的动态变化。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2024-10-10 DOI: 10.1111/dgd.12945

Laura I. Arbanas, Emanuel Cura Costa, Osvaldo Chara, Leo Otsuki, Elly M. Tanaka

{"title":"Lineage tracing of Shh+ floor plate cells and dynamics of dorsal–ventral gene expression in the regenerating axolotl spinal cord","authors":"Laura I. Arbanas, Emanuel Cura Costa, Osvaldo Chara, Leo Otsuki, Elly M. Tanaka","doi":"10.1111/dgd.12945","DOIUrl":"10.1111/dgd.12945","url":null,"abstract":"Both development and regeneration depend on signaling centers, which are sources of locally secreted tissue-patterning molecules. As many signaling centers are decommissioned before the end of embryogenesis, a fundamental question is how signaling centers can be re-induced later in life to promote regeneration after injury. Here, we use the axolotl salamander model (Ambystoma mexicanum) to address how the floor plate is assembled for spinal cord regeneration. The floor plate is an archetypal vertebrate signaling center that secretes Shh ligand and patterns neural progenitor cells during embryogenesis. Unlike mammals, axolotls continue to express floor plate genes (including Shh) and downstream dorsal–ventral patterning genes in their spinal cord throughout life, including at steady state. The parsimonious hypothesis that Shh+ cells give rise to functional floor plate cells for regeneration had not been tested. Using HCR in situ hybridization and mathematical modeling, we first quantified the behaviors of dorsal–ventral spinal cord domains, identifying significant increases in gene expression level and floor plate size during regeneration. Next, we established a transgenic axolotl to specifically label and fate map Shh+ cells in vivo. We found that labeled Shh+ cells gave rise to regeneration floor plate, and not to other neural progenitor domains, after tail amputation. Thus, despite changes in domain size and downstream patterning gene expression, Shh+ cells retain their floor plate identity during regeneration, acting as a stable cellular source for this regeneration signaling center in the axolotl spinal cord.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 8","pages":"414-425"},"PeriodicalIF":1.7,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12945","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142479803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment and characterization of mouse lines useful for endogenous protein degradation via an improved auxin-inducible degron system (AID2) 通过改良的辅助素诱导降解子系统（AID2），建立有助于内源性蛋白质降解的小鼠品系并确定其特征。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2024-09-21 DOI: 10.1111/dgd.12942

Hatsune Makino-Itou, Noriko Yamatani, Akemi Okubo, Makoto Kiso, Rieko Ajima, Masato T. Kanemaki, Yumiko Saga

{"title":"Establishment and characterization of mouse lines useful for endogenous protein degradation via an improved auxin-inducible degron system (AID2)","authors":"Hatsune Makino-Itou, Noriko Yamatani, Akemi Okubo, Makoto Kiso, Rieko Ajima, Masato T. Kanemaki, Yumiko Saga","doi":"10.1111/dgd.12942","DOIUrl":"10.1111/dgd.12942","url":null,"abstract":"The development of new technologies opens new avenues in the research field. Gene knockout is a key method for analyzing gene function in mice. Currently, conditional gene knockout strategies are employed to examine temporal and spatial gene function. However, phenotypes are sometimes not observed because of the time required for depletion due to the long half-life of the target proteins. Protein knockdown using an improved auxin-inducible degron system, AID2, overcomes such difficulties owing to rapid and efficient target depletion. We observed depletion of AID-tagged proteins within a few to several hours by a simple intraperitoneal injection of the auxin analog, 5-Ph-IAA, which is much shorter than the time required for target depletion using conditional gene knockout. Importantly, the loss of protein is reversible, making protein knockdown useful to measure the effects of transient loss of protein function. Here, we also established several mouse lines useful for AID2-medicated protein knockdown, which include knock-in mouse lines in the ROSA26 locus; one expresses TIR1(F74G), and the other is the reporter expressing AID-mCherry. We also established a germ-cell-specific TIR1 line and confirmed the protein knockdown specificity. In addition, we introduced an AID tag to an endogenous protein, DCP2 via the CAS9-mediated gene editing method. We confirmed that the protein was effectively eliminated by TIR1(F74G), which resulted in the similar phenotype observed in knockout mouse within 20 h.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 7","pages":"384-393"},"PeriodicalIF":1.7,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12942","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142300049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chicken embryo cultures in the dorsal-upward orientation for the manipulation of epiblasts 鸡胚背向上方培养用于操作上胚层

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2024-09-17 DOI: 10.1111/dgd.12943

Kaho Konya, Yusaku Watanabe, Akihito Kawamura, Kae Nakamura, Hideaki Iida, Koya Yoshihi, Hisato Kondoh

{"title":"Chicken embryo cultures in the dorsal-upward orientation for the manipulation of epiblasts","authors":"Kaho Konya, Yusaku Watanabe, Akihito Kawamura, Kae Nakamura, Hideaki Iida, Koya Yoshihi, Hisato Kondoh","doi":"10.1111/dgd.12943","DOIUrl":"10.1111/dgd.12943","url":null,"abstract":"Chicken embryos have many advantages in the study of amniote embryonic development. In particular, culture techniques developed for early-stage embryos have promoted the advancement of modern developmental studies using chicken embryos. However, the standard technique involves placing chicken embryos in the ventral-upward (ventral-up) orientation, limiting manipulation of the epiblast at the dorsal surface, which is the primary source of ectodermal and mesodermal tissues. To circumvent this limitation, we developed chicken embryo cultures in the dorsal-up orientation and exploited this technique to address diverse issues. In this article, we first review the history of chicken embryo culture techniques to evaluate the advantages and limitations of the current standard technique. Then, the dorsal-up technique is discussed. These technological discussions are followed by three different examples of experimental analyses using dorsal-up cultures to illustrate their advantages: (1) EdU labeling of epiblast cells to assess potential variation in the cell proliferation rate; (2) migration behaviors of N1 enhancer-active epiblast cells revealed by tracking cells with focal fluorescent dye labeling in dorsal-up embryo culture; and (3) neural crest development of mouse neural stem cells in chicken embryos.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 8","pages":"426-434"},"PeriodicalIF":1.7,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12943","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142257501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meeting report of the 57th Annual Meeting of the Japanese Society for Developmental Biologists 日本发育生物学家学会第 57 届年会会议报告

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2024-09-10 DOI: 10.1111/dgd.12941

Yuzuka Takeuchi

引用次数: 0

Development of specialized devices for microbial experimental evolution 开发微生物实验进化专用设备。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2024-08-26 DOI: 10.1111/dgd.12940

Atsushi Shibai, Chikara Furusawa

引用次数: 0

The self-in-the-world map emerged in the primate brain as a basis for Homo sapiens abilities 自我世界地图出现在灵长类大脑中，是智人能力的基础。

IF 1.7 4区生物学

Development Growth & Differentiation Pub Date : 2024-08-08 DOI: 10.1111/dgd.12939

Rafael Bretas, Banty Tia, Atsushi Iriki

{"title":"The self-in-the-world map emerged in the primate brain as a basis for Homo sapiens abilities","authors":"Rafael Bretas, Banty Tia, Atsushi Iriki","doi":"10.1111/dgd.12939","DOIUrl":"10.1111/dgd.12939","url":null,"abstract":"The brain in the genus Homo expanded rapidly during evolution, accelerated by a reciprocated interaction between neural, cognitive, and ecological niches (triadic niche construction, or TNC). This biologically costly expansion incubated latent cognitive capabilities that, with a quick and inexpensive rewiring of brain areas in a second phase of TNC, provided the basis for Homo sapiens specific abilities. The neural demands for perception of the human body in interaction with tools and the environment required highly integrated sensorimotor domains, inducing the parietal lobe expansion seen in humans. These newly expanded brain areas allowed connecting the sensations felt in the body to the actions in the world through the cognitive function of “projection”. In this opinion article, we suggest that as a relationship of equivalence between body parts, tools and their external effects was established, mental mechanisms of self-objectification might have emerged as described previously, grounding notions of spatial organization, idealized objects, and their transformations, as well as socio-emotional states in the sensing agent through a self-in-the-world map. Therefore, human intelligence and its features such as symbolic thought, language, mentalizing, and complex technical and social behaviors could have stemmed from the explicit awareness of the causal relationship between the self and intentional modifications to the environment.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 6","pages":"342-348"},"PeriodicalIF":1.7,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12939","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141903468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0