The long-term survival of enteroendocrine cells depends on their subtype and is linked to peripheral sensory innervation

IF 1.7 4区 生物学 Q4 CELL BIOLOGY
Salsabila Luthfi Sesotyosari, Masato Kinoshita, Mukhamad Sunardi, Mo Lihan, Akimasa Orii, Takaya Abe, Hiroshi Kiyonari, Tatsuya Nakai, Toshihiro Uesaka, Yuzo Kodama, Hideki Enomoto
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Abstract

Enteroendocrine cells (EECs) are sensory epithelial cells that sense the gut luminal environment and convey sensory information to the brain via the visceral afferent pathway. Although EECs are a part of gut epithelial cells, which generally undergo rapid turnover, some EECs have been reported to be long-lived. EECs consist of multiple subtypes, each of which displays distinct hormone production and distribution patterns. It remains unknown whether a long lifespan is a characteristic shared by all EEC subtypes. To address this issue, we conducted genetic pulse labeling of three EEC subtypes expressing serotonin (5-HT), peptide YY (PYY), and gastric inhibitory polypeptide (GIP) in mice and tracked their survival. In the proximal small intestine, all labeled GIP+ EECs disappeared completely within 5 days, whereas some PYY+ EECs survived for more than 7 days. In the proximal colon, some labeled 5-HT+ EECs lived for more than 28 days, whereas no PYY+ cells survived beyond 14 days. These long-lived 5-HT+ EECs were almost exclusively found in the upper half of the crypt in the mucosal fold, where visceral sensory fibers were enriched. This study reveals subtype- and region-dependent survival of EECs and suggests that EEC–nerve communication may underlie the long lifespan of certain EECs.

肠内分泌细胞的长期存活取决于其亚型,并与外周感觉神经支配有关。
肠内分泌细胞(EECs)是一种感觉上皮细胞,它能感知肠道环境,并通过内脏传入途径将感觉信息传递给大脑。虽然EECs是肠道上皮细胞的一部分,通常经历快速的更新,但一些EECs有报道是长寿命的。EECs由多个亚型组成,每个亚型都表现出不同的激素产生和分布模式。长寿命是否是所有EEC亚型共有的特征尚不清楚。为了解决这一问题,我们对小鼠中表达5-羟色胺(5-HT)、YY肽(PYY)和胃抑制多肽(GIP)的三种EEC亚型进行了遗传脉冲标记,并追踪了它们的存活情况。在小肠近端,所有标记的GIP+ EECs在5天内完全消失,而一些标记的PYY+ EECs存活超过7天。在近端结肠中,一些标记的5-HT+ EECs存活超过28天,而PYY+细胞没有存活超过14天。这些长寿命的5-HT+ EECs几乎只存在于粘膜褶隐窝的上半部分,在那里内脏感觉纤维丰富。这项研究揭示了脑脊膜细胞的亚型和区域依赖性存活,并表明脑脊膜细胞-神经通讯可能是某些脑脊膜细胞长寿的基础。
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来源期刊
Development Growth & Differentiation
Development Growth & Differentiation 生物-发育生物学
CiteScore
4.60
自引率
4.00%
发文量
62
审稿时长
6 months
期刊介绍: Development Growth & Differentiation (DGD) publishes three types of articles: original, resource, and review papers. Original papers are on any subjects having a context in development, growth, and differentiation processes in animals, plants, and microorganisms, dealing with molecular, genetic, cellular and organismal phenomena including metamorphosis and regeneration, while using experimental, theoretical, and bioinformatic approaches. Papers on other related fields are also welcome, such as stem cell biology, genomics, neuroscience, Evodevo, Ecodevo, and medical science as well as related methodology (new or revised techniques) and bioresources. Resource papers describe a dataset, such as whole genome sequences and expressed sequence tags (ESTs), with some biological insights, which should be valuable for studying the subjects as mentioned above. Submission of review papers is also encouraged, especially those providing a new scope based on the authors’ own study, or a summarization of their study series.
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