Development Growth & Differentiation最新文献

{"title":"Endothelial cell transitions in zebrafish vascular development","authors":"Li-Kun Phng,&nbsp;Benjamin M. Hogan","doi":"10.1111/dgd.12938","DOIUrl":"10.1111/dgd.12938","url":null,"abstract":"<p>In recent decades, developmental biologists have come to view vascular development as a series of progressive transitions. Mesoderm differentiates into endothelial cells; arteries, veins and lymphatic endothelial cells are specified from early endothelial cells; and vascular networks diversify and invade developing tissues and organs. Our understanding of this elaborate developmental process has benefitted from detailed studies using the zebrafish as a model system. Here, we review a number of key developmental transitions that occur in zebrafish during the formation of the blood and lymphatic vessel networks.</p>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 6","pages":"357-368"},"PeriodicalIF":1.7,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12938","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141769928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zebrafish trpm7 mutants show reduced motility in free movement","authors":"Kenta Watai,&nbsp;Kenichiro Sadamitsu,&nbsp;Seiji Wada,&nbsp;Makoto Kashima,&nbsp;Hiromi Hirata","doi":"10.1111/dgd.12937","DOIUrl":"10.1111/dgd.12937","url":null,"abstract":"<p>Parkinson's disease is a neurological disorder characterized by reduced motility, depression and dementia. Guamanian parkinsonism dementia with amyotrophic sclerosis is a local case of Parkinson's disease reported in the Western Pacific Islands of Guam and Rota as well as in the Kii Peninsula of Japan. A previous genetic study has suggested that Guamanian parkinsonism is attributable to a variant of the <i>TRPM7</i> gene, which encodes for melastatin-related transient receptor potential (TRP) ion channels. But the link between parkinsonism and the <i>TRPM7</i> gene remains elusive. Previous studies have addressed that <i>trpm7</i>-deficient zebrafish embryos showed defects in pigmentation and touch-evoked motor response. In this study, we identified a new viable allele of <i>trpm7</i> mutant causing an I756N amino acid substitution in the first transmembrane domain. Behavioral analyses revealed that <i>trpm7</i> mutants showed compromised motility with their movement distance shorter than wild-type larvae. The velocity of the movement was significantly reduced in <i>trpm7</i> mutants than in wild-type larvae. Along with a previous finding of reduced dopaminergic neurons in zebrafish <i>trpm7</i> mutants, reduced motility of <i>trpm7</i> mutants can suggest another similarity between <i>trpm7</i> phenotypes and Parkinson's disease symptoms.</p>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 6","pages":"349-356"},"PeriodicalIF":1.7,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141545503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential cellular stiffness across tissues that contribute to Xenopus neural tube closure","authors":"Makoto Suzuki,&nbsp;Naoko Yasue,&nbsp;Naoto Ueno","doi":"10.1111/dgd.12936","DOIUrl":"10.1111/dgd.12936","url":null,"abstract":"<p>During the formation of the neural tube, the primordium of the vertebrate central nervous system, the actomyosin activity of cells in different regions drives neural plate bending. However, how the stiffness of the neural plate and surrounding tissues is regulated and mechanically influences neural plate bending has not been elucidated. Here, we used atomic force microscopy to reveal the relationship between the stiffness of the neural plate and the mesoderm during <i>Xenopus</i> neural tube formation. Measurements with intact embryos revealed that the stiffness of the neural plate was consistently higher compared with the non-neural ectoderm and that it increased in an actomyosin activity-dependent manner during neural plate bending. Interestingly, measurements of isolated tissue explants also revealed that the relationship between the stiffness of the apical and basal sides of the neural plate was reversed during bending and that the stiffness of the mesoderm was lower than that of the basal side of the neural plate. The experimental elevation of mesoderm stiffness delayed neural plate bending, suggesting that low mesoderm stiffness mechanically supports neural tube closure. This study provides an example of mechanical interactions between tissues during large-scale morphogenetic movements.</p>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 5","pages":"320-328"},"PeriodicalIF":1.7,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12936","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141460539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel preparation for histological analyses of intraventricular macrophages in the embryonic brain","authors":"Futoshi Murayama,&nbsp;Hisa Asai,&nbsp;Arya Kirone Patra,&nbsp;Hiroaki Wake,&nbsp;Takaki Miyata,&nbsp;Yuki Hattori","doi":"10.1111/dgd.12935","DOIUrl":"10.1111/dgd.12935","url":null,"abstract":"<p>Microglia colonize the brain starting on embryonic day (E) 9.5 in mice, and their population increases with development. We have previously demonstrated that some microglia are derived from intraventricular macrophages, which frequently infiltrate the pallium at E12.5. To address how the infiltration of intraventricular macrophages is spatiotemporally regulated, histological analyses detecting how these cells associate with the surrounding cells at the site of infiltration into the pallial surface are essential. Using two-photon microscopy-based in vivo imaging, we demonstrated that most intraventricular macrophages adhere to the ventricular surface. This is a useful tool for imaging intraventricular macrophages maintaining their original position, but this method cannot be used for observing deeper brain regions. Meanwhile, we found that conventional cryosection-based and naked pallial slice-based observation resulted in unexpected detachment from the ventricular surface of intraventricular macrophages and their mislocation, suggesting that previous histological analyses might have failed to determine their physiological number and location in the ventricular space. To address this, we sought to establish a methodological preparation that enables us to delineate the structure and cellular interactions when intraventricular macrophages infiltrate the pallium. Here, we report that brain slices pretreated with agarose-embedding maintained adequate density and proper positioning of intraventricular macrophages on the ventricular surface. This method also enabled us to perform the immunostaining. We believe that this is helpful for conducting histological analyses to elucidate the mechanisms underlying intraventricular macrophage infiltration into the pallium and their cellular properties, leading to further understanding of the process of microglial colonization into the developing brain.</p>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 5","pages":"329-337"},"PeriodicalIF":1.7,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12935","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141421768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An analysis of semaphorin-mediated cellular interactions in the Caenorhabditis elegans epidermis using the IR-LEGO single-cell gene induction system","authors":"Motoshi Suzuki,&nbsp;Shin Takagi","doi":"10.1111/dgd.12925","DOIUrl":"10.1111/dgd.12925","url":null,"abstract":"<p>One of the major functions of the semaphorin signaling system is the regulation of cell shape. In the nematode <i>Caenorhabditis elegans</i>, membrane-bound semaphorins SMP-1/2 (SMPs) regulate the morphology of epidermal cells via their receptor plexin, PLX-1. In the larval male tail of the SMP-PLX-1 signaling mutants, the border between two epidermal cells, R1.p and R2.p, is displaced anteriorly, resulting in the anterior displacement of the anterior-most ray, ray 1, in the adult male. To elucidate how the intercellular signaling mediated by SMPs regulates the position of the intercellular border, we performed mosaic gene expression analyses by using infrared laser-evoked gene operator (IR-LEGO). We show that PLX-1 expressed in R1.p and SMP-1 expressed in R2.p are required for the proper positioning of ray 1. The result suggests that SMP signaling promotes extension, rather than retraction, of R1.p. This is in contrast to a previous finding that SMPs mediate inhibition of cell extension of vulval precursor cells, another group of epidermal cells of <i>C. elegans</i>, indicating the context dependence of cell shape control via the semaphorin signaling system.</p>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 5","pages":"308-319"},"PeriodicalIF":1.7,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12925","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140960844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assembly of continuous high-resolution draft genome sequence of Hemicentrotus pulcherrimus using long-read sequencing","authors":"Tetsushi Komoto,&nbsp;Kazuho Ikeo,&nbsp;Shunsuke Yaguchi,&nbsp;Takashi Yamamoto,&nbsp;Naoaki Sakamoto,&nbsp;Akinori Awazu","doi":"10.1111/dgd.12924","DOIUrl":"10.1111/dgd.12924","url":null,"abstract":"<p>The update of the draft genome assembly of sea urchin, <i>Hemicentrotus pulcherrimus</i>, which is widely studied in East Asia as a model organism of early development, was performed using Oxford nanopore long-read sequencing. The updated assembly provided ~600-Mb genome sequences divided into 2,163 contigs with N50 = 516 kb. BUSCO completeness score and transcriptome model mapping ratio (TMMR) of the present assembly were obtained as 96.5% and 77.8%, respectively. These results were more continuous with higher resolution than those by the previous version of <i>H. pulcherrimus</i> draft genome, HpulGenome_v1, where the number of scaffolds = 16,251 with a total of ~100 Mb, N50 = 143 kb, BUSCO completeness score = 86.1%, and TMMR = 55.4%. The obtained genome contained 36,055 gene models that were consistent with those in other echinoderms. Additionally, two tandem repeat sequences of early histone gene locus containing 47 copies and 34 copies of all histone genes, and 185 of the homologous sequences of the interspecifically conserved region of the <i>Ars</i> insulator, ArsInsC, were obtained. These results provide further advance for genome-wide research of development, gene regulation, and intranuclear structural dynamics of multicellular organisms using <i>H. pulcherrimus</i>.</p>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 4","pages":"297-304"},"PeriodicalIF":2.5,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12924","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140623871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epithelial–mesenchymal transition contrast in the amputated tail and limb of the northern house gecko, Hemidactylus flaviviridis","authors":"Pooja Raval,&nbsp;Kashmira Khaire,&nbsp;Shashikant Sharma,&nbsp;Suresh Balakrishnan","doi":"10.1111/dgd.12923","DOIUrl":"10.1111/dgd.12923","url":null,"abstract":"<p>The northern house gecko <i>Hemidactylus flaviviridis</i> exhibits appendage-specific responses to injuries. The autotomized tail regenerates, whereas the severed limb fails to regrow. Many site-specific cellular processes influence tail regeneration. Herein, we analyzed the epithelial–mesenchymal transition contrast in the lizard's amputated appendages (tail and limb). Morphological observations in the healing frame indicated the formation of regeneration blastema in the tail and scar formation in limb. Histology of the tail showed that epithelial cells closer to mesenchyme appeared less columnar and loosely packed, with little intercellular matrix. Whereas in the limb, the columnar epithelial cells remained tightly packed. Collagen deposition was seen in the limb at the intersection of wound epithelium and mesenchyme, favoring scarring by blocking the epithelial–mesenchymal transition. Markers for epithelial–mesenchymal transition were assessed at transcript and protein levels. The regenerating tail showed upregulation of N-cadherin, vimentin, and PCNA, favoring epithelial–mesenchymal transition, cell migration, and proliferation, respectively. In contrast, the scarring limb showed persistently elevated levels of E-cadherin and EpCAM, indicating retention of epithelial characteristics. An attempt was made to screen the resident epithelial stem cell population in both appendages to check their potential role in the epithelial–mesenchymal transition (EMT), hence the differential wound healing. Upregulation in transcript and protein levels of Nanog and Sox2 was observed in the regenerating tail. Fluorescence-activated cell sorting (FACS) provided supporting evidence that the epithelial stem cell population in tail remained significantly higher than in limb. Thus, this study focuses on the mechanistic role of the epithelial–mesenchymal transition in wound healing, highlighting the molecular details of regeneration and scarring events.</p>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 4","pages":"285-296"},"PeriodicalIF":2.5,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140566998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptomic characterization of the ovary in the spawning process of the mud crab Scylla paramamosain","authors":"Shisheng Tu,&nbsp;Guohong Yu,&nbsp;Fuqiang Ge,&nbsp;Rui Xu,&nbsp;Zhongwen Jin,&nbsp;Xi Xie,&nbsp;Dongfa Zhu","doi":"10.1111/dgd.12921","DOIUrl":"10.1111/dgd.12921","url":null,"abstract":"<p>Oviposition is induced upon mating in most insects. Spawning is a physiological process that is fundamental for the reproduction of <i>Scylla paramamosain</i>. However, the molecular mechanisms underlying the spawning process in this species are poorly understood. Herein, comprehensive ovary transcriptomic analysis was conducted at the germinal vesicle breakdown stage (GVBD), spawning stage, 0.5 h post-spawning stage, and 24 h post-spawning stage of <i>S. paramamosain</i> for gene discovery. A total of 67,230 unigenes were generated, and 27,975 (41.61%) unigenes were annotated. Meanwhile, the differentially expressed genes (DEGs) between the different groups were identified, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was subsequently conducted. These results suggested that octopamine (OA) and tyramine (TA) could induce oviposition, while dopamine (DA) and serotonin (5-hydroxytryptamine [5-HT]) inhibit oviposition. The 20-hydroxyecdysone (20E) and methyl farnesoate (MF) signal pathways might be positively associated with oviposition. Furthermore, numerous transcripts that encode neuropeptides and their G-protein-coupled receptors (GPCRs), such as CNMamide, RYamide, ecdysis-triggering hormone (ETH), GPA2/GPB5 receptor, and Moody receptor, appear to be differentially expressed during the spawning process. Eleven unigenes were selected for qRT-PCR and the pattern was found to be consistent with the transcriptome expression pattern. Our work is the first spawning-related investigation of <i>S. paramamosain</i> focusing on the ovary at the whole transcriptome level. These findings assist in improving our understanding of spawning regulation in <i>S. paramamosain</i> and provide information for oviposition studies in other crustaceans.</p>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 4","pages":"274-284"},"PeriodicalIF":2.5,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140159399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “maea affects head formation through β-catenin degradation during early Xenopus laevis development”","authors":"","doi":"10.1111/dgd.12920","DOIUrl":"10.1111/dgd.12920","url":null,"abstract":"&lt;p&gt;Goto, T., &amp; Shibuya, H. (2023). &lt;i&gt;maea&lt;/i&gt; affects head formation through β-catenin degradation during early &lt;i&gt;Xenopus laevis&lt;/i&gt; development. &lt;i&gt;Development, Growth &amp; Differentiation&lt;/i&gt;, 65(1), 29–36. https://doi.org/10.1111/dgd.12828&lt;/p&gt;&lt;p&gt;In this article, the German letter “Eszett: ß” was used where the Greek letter “beta: β” should have been used in all cases.&lt;/p&gt;&lt;p&gt;The following points need to be corrected:&lt;/p&gt;&lt;p&gt;In the title,&lt;/p&gt;&lt;p&gt;“&lt;i&gt;maea&lt;/i&gt; affects head formation through β-catenin degradation during early &lt;i&gt;Xenopus laevis&lt;/i&gt; development”&lt;/p&gt;&lt;p&gt;In the Abstract,&lt;/p&gt;&lt;p&gt;“β-Catenin protein stability is a key factor in canonical Wnt signaling.”&lt;/p&gt;&lt;p&gt;“Several E3 ubiquitin ligases contribute to β-catenin degradation through the ubiquitin/proteasome system.”&lt;/p&gt;&lt;p&gt;“The expression levels of the Wnt target genes &lt;i&gt;nodal homolog 3&lt;/i&gt;, &lt;i&gt;gene 1&lt;/i&gt; (&lt;i&gt;nodal3.1&lt;/i&gt;), and &lt;i&gt;siamois homeodomain 1&lt;/i&gt; (&lt;i&gt;sia1&lt;/i&gt;), which were induced by injection with &lt;i&gt;β-catenin&lt;/i&gt; mRNA, were reduced by maea.S mRNA co-injection. maea.S overexpression at the anterior dorsal region enlarged head structures, whereas Maea knockdown interfered with head formation in &lt;i&gt;Xenopus&lt;/i&gt; embryos.”&lt;/p&gt;&lt;p&gt;“Maea.S decreased and ubiquitinated β-catenin protein.”&lt;/p&gt;&lt;p&gt;“β-catenin-4KRs protein, which mutated the four lysine (K) residues known as ubiquitinated sites to arginine (R) residues, was also ubiquitinated and degraded by Maea.S.”&lt;/p&gt;&lt;p&gt;In the KEYWORDS,&lt;/p&gt;&lt;p&gt;“degradation, maea, β-catenin, ubiquitination, &lt;i&gt;Xenopus laevis&lt;/i&gt;”&lt;/p&gt;&lt;p&gt;In the INTRODUCTION (first paragraph),&lt;/p&gt;&lt;p&gt;“The key aspect of Wnt signalling is β-catenin protein stability. Disheveled segment polarity protein (Dvl) is recruited at the cell membrane and prevents β-catenin degradation under the Wnt-on state.”&lt;/p&gt;&lt;p&gt;“Under the Wnt-off state, Axin1, adenomatous polyposis coli (Apc), casein kinase 1 alpha 1 (Csnk1α1), and glycogen synthase kinase 3 beta (Gsk3β) form the destruction complex to phosphorylate β-catenin protein (Liu et al., 2002).”&lt;/p&gt;&lt;p&gt;“Phosphorylated β-catenin is ubiquitinated by E3 ubiquitin ligases, such as beta-transducin repeat-containing E3 ubiquitin-protein ligase (Btrc), and is then degraded by the proteasome system.”&lt;/p&gt;&lt;p&gt;In the INTRODUCTION (third paragraph),&lt;/p&gt;&lt;p&gt;“There are four lysine residues known as ubiquitinated sites in β-catenin protein. Both lysine residues 19 and 49 are ubiquitinated by Btrc (Winer et al., 2006) and jade family PHD finger 1 (Jade1) (Chitalia et al., 2008).”&lt;/p&gt;&lt;p&gt;“Additionally, Siah E3 ubiquitin-protein ligase 1 (Siah1) ubiquitinates β-catenin at lysine residues 666 and 671 (Dimitrova et al., 2010).”&lt;/p&gt;&lt;p&gt;“HECT, UBA, and WWE domain containing E3 ubiquitin protein ligase 1 (Huwe1) and SNF2 histone linker PHD RING helicase, E3 ubiquitin protein ligase (Shprh) are also related to β-catenin protein degradation, but the sites they ubiquitinate have not been identified (Dominguez-Brauer et al., 2017; Qu et al., 2016).”&lt;/p&gt;&lt;p&gt;In the INTRODUCTIO","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 3","pages":"266-270"},"PeriodicalIF":2.5,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12920","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140159400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic screening of fish-specific genes in gnathostomes and their functions in fin development","authors":"Hidehiro Kudoh,&nbsp;Sayuri Yonei-Tamura,&nbsp;Gembu Abe,&nbsp;Junichi Iwakiri,&nbsp;Masahiro Uesaka,&nbsp;Takashi Makino,&nbsp;Koji Tamura","doi":"10.1111/dgd.12918","DOIUrl":"10.1111/dgd.12918","url":null,"abstract":"<p>In this study, we comprehensively searched for fish-specific genes in gnathostomes that contribute to development of the fin, a fish-specific trait. Many previous reports suggested that animal group-specific genes are often important for group-specific traits. Clarifying the roles of fish-specific genes in fin development of gnathostomes, for example, can help elucidate the mechanisms underlying the formation of this trait. We first identified 91 fish-specific genes in gnathostomes by comparing the gene repertoire in 16 fish and 35 tetrapod species. RNA-seq analysis narrowed down the 91 candidates to 33 genes that were expressed in the developing pectoral fin. We analyzed the functions of approximately half of the candidate genes by loss-of-function analysis in zebrafish. We found that some of the fish-specific and fin development-related genes, including <i>fgf24</i> and <i>and1</i>/<i>and2</i>, play roles in fin development. In particular, the newly identified fish-specific gene <i>qkia</i> is expressed in the developing fin muscle and contributes to muscle morphogenesis in the pectoral fin as well as body trunk. These results indicate that the strategy of identifying animal group-specific genes is functional and useful. The methods applied here could be used in future studies to identify trait-associated genes in other animal groups.</p>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 3","pages":"235-247"},"PeriodicalIF":2.5,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12918","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140029426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}