Development Growth & Differentiation最新文献_第2页

Computer-Aided Sperm Analysis Protocol for Evaluating Sperm Motility in Japanese Medaka 评估日本Medaka地区精子活力的计算机辅助精子分析方案。

IF 1 4区生物学

Development Growth & Differentiation Pub Date : 2026-06-10 DOI: 10.1111/dgd.70061

Miwa Kuroyanagi, Satoshi Ansai, Keigo Okamoto, Ai Kato, Touko Yamazaki, Yasuhiro Kamei, Eiji Watanabe, Kiyoshi Naruse, Yukiko Ogino

{"title":"Computer-Aided Sperm Analysis Protocol for Evaluating Sperm Motility in Japanese Medaka","authors":"Miwa Kuroyanagi, Satoshi Ansai, Keigo Okamoto, Ai Kato, Touko Yamazaki, Yasuhiro Kamei, Eiji Watanabe, Kiyoshi Naruse, Yukiko Ogino","doi":"10.1111/dgd.70061","DOIUrl":"10.1111/dgd.70061","url":null,"abstract":"Changes in sperm motility can serve as an early indicator of reproductive effects caused by environmental chemicals or genetic perturbations. However, sperm motility is highly sensitive to external factors such as osmolarity, ionic composition, and the timing of measurement after activation, making it challenging to obtain consistent and reproducible measurements. Here, we present a standardized protocol for assessing sperm motility in Japanese medaka (Oryzias latipes) using a sperm motility analysis system (SMAS), an application for computer-aided sperm motility analysis (CASA). This protocol details the procedures for sperm collection, activation, and quantitative motility assessment, with particular focus on changes in the percentage of motile sperm post activation and the effects of sperm cryopreservation. We demonstrate time-dependent declines in sperm motility and velocity, and highlight the importance of early post-activation measurements to accurately capture peak motility. Notably, cryopreservation significantly accelerated the decline in sperm motility rate without affecting the initial proportion of motile sperm. To enable reliable comparisons among experimental groups, we recommend standardizing the initiation time after sperm activation by using CASA, and show that measurements should be initiated within 1 min after activation to obtain consistent and reliable data. This standardized SMAS-based protocol provides a robust and reproducible framework for sperm motility analysis in medaka and will be valuable not only for studies in reproductive biology, toxicology, and environmental risk assessment but also for applied research, such as breeding of aquacultural fishes.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 4-5","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.70061","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148220787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Atypical Cadherin Fat2 is Involved in Axogenesis of Cerebellar Granule Cells in Zebrafish 非典型钙粘蛋白Fat2参与斑马鱼小脑颗粒细胞的轴生

IF 1 4区生物学

Development Growth & Differentiation Pub Date : 2026-06-09 DOI: 10.1111/dgd.70060

Jui Chun Wang, Miki Takeuchi, Ryuji Dohaku, Seok-Hyung Kim, Lilianna Solnica-Krezel, Takashi Shimizu, Masahiko Hibi

{"title":"Atypical Cadherin Fat2 is Involved in Axogenesis of Cerebellar Granule Cells in Zebrafish","authors":"Jui Chun Wang, Miki Takeuchi, Ryuji Dohaku, Seok-Hyung Kim, Lilianna Solnica-Krezel, Takashi Shimizu, Masahiko Hibi","doi":"10.1111/dgd.70060","DOIUrl":"https://doi.org/10.1111/dgd.70060","url":null,"abstract":"Fat2 is an atypical cadherin with a large extracellular domain containing multiple cadherin repeats. Although members of the Fat family have been implicated in organ development, the role of Fat2 in neural development remains poorly understood. Here, we investigated the function of Fat2 in zebrafish, focusing on its involvement in the development of cerebellar granule cells. Granule cells in the rostromedial cerebellum project their axons, known as parallel fibers, to Purkinje cell dendrites. In contrast, granule cells in the caudolateral cerebellum project their axons to Purkinje cells and further extend them caudally to innervate crest cells, Purkinje-like neurons located in the dorsal hindbrain region called the medio-octaval lateralis. We found that in fat2 mutants, axons of caudolateral cerebellar granule cells projecting to crest cells exhibit abnormal branching and frequently overshoot their targets. These results indicate that Fat2 is required for proper axon formation in at least a subset of cerebellar granule cells.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 4-5","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.70060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148209012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 1 4区生物学

Development Growth & Differentiation Pub Date : 2026-04-17

引用次数: 0

IF 1 4区生物学

Development Growth & Differentiation Pub Date : 2026-04-17

引用次数: 0

IF 1 4区生物学

Development Growth & Differentiation Pub Date : 2026-04-15

引用次数: 0

IF 1 4区生物学

Development Growth & Differentiation Pub Date : 2026-04-15

引用次数: 0

Derepression of a Subset of Meiotic Proteins in Primordial Germ Cells of max Mutant Zebrafish. 最大突变斑马鱼原始生殖细胞中减数分裂蛋白亚群的表达。

IF 1.5 4区生物学

Development Growth & Differentiation Pub Date : 2026-04-01 DOI: 10.1111/dgd.70053

Yuya Hasegawa, Toshihiro Kawasaki, Yukiko Imai, Yasuhisa Matsui, Noriyoshi Sakai

{"title":"Derepression of a Subset of Meiotic Proteins in Primordial Germ Cells of max Mutant Zebrafish.","authors":"Yuya Hasegawa, Toshihiro Kawasaki, Yukiko Imai, Yasuhisa Matsui, Noriyoshi Sakai","doi":"10.1111/dgd.70053","DOIUrl":"10.1111/dgd.70053","url":null,"abstract":"Germ cells in zebrafish are specified by a preformation mechanism, unlike in mice. Max (Myc associated factor X) and its associated PRC1.6 (polycomb repressive complex 1.6) act as negative regulators of germ cell- and meiosis-related genes in mice; however, their function in zebrafish remains unknown. Here, we generated max mutants in zebrafish using genome-editing techniques to investigate the role of Max in germ cell development and meiosis. No obvious morphological abnormalities were observed in the mutants up to 4 days post fertilization (dpf), likely due to maternally supplied max mRNAs. However, the mutants failed to form an inflated swim bladder and gradually died after 10 dpf. The number of primordial germ cells (PGCs) did not differ between the max mutant and wild-type larvae up to 12 dpf. In contrast, analysis of protein expression levels of germ cell-related and meiosis-related genes in PGCs at 9 dpf revealed that the levels of Sycp2, Sycp3, and Dmc1 proteins were significantly increased in max mutants, whereas the expression of Ddx4 and Piwil1 remained unchanged. Together, these results suggest that zebrafish Max selectively represses the expression of a subset of meiotic genes in PGCs.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 3","pages":"e70053"},"PeriodicalIF":1.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147693677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cooperative Roles of Pds5a and Pds5b Constrain Long-Range Chromatin Interactions in Vertebrate Embryos. Pds5a和Pds5b的合作作用限制了脊椎动物胚胎中染色质的远程相互作用。

IF 1.5 4区生物学

Development Growth & Differentiation Pub Date : 2026-04-01 DOI: 10.1111/dgd.70054

Shinra Ikeda, Hiroyuki Takeda, Ryohei Nakamura

{"title":"Cooperative Roles of Pds5a and Pds5b Constrain Long-Range Chromatin Interactions in Vertebrate Embryos.","authors":"Shinra Ikeda, Hiroyuki Takeda, Ryohei Nakamura","doi":"10.1111/dgd.70054","DOIUrl":"10.1111/dgd.70054","url":null,"abstract":"Three-dimensional (3D) genome organization is dynamically restructured during early vertebrate development, yet how chromatin domains are established remains poorly understood. In particular, the contribution of individual cohesin regulators to this process during embryogenesis is unclear. PDS5 proteins are key modulators of cohesin dynamics, but their depletion has been reported to cause context-dependent and sometimes contrasting architectural effects in cultured cells. Here, we investigated the roles of the cohesin regulators Pds5a and Pds5b during early development using the medaka embryo. Developmental transcriptome analysis revealed distinct but overlapping expression dynamics of pds5a and pds5b around the transition from zygotic genome activation to gastrulation. Morpholino-mediated depletion of either paralog resulted in only mild morphological phenotypes, whereas simultaneous depletion caused more severe developmental defects. In situ Hi-C analysis showed that single depletion of pds5a or pds5b induced only modest changes in 3D genome organization. In contrast, double depletion led to pronounced architectural alterations, including increased long-range chromatin contacts and de novo formation of extended chromatin loops. Transcriptome analysis revealed largely shared, with some condition-specific, gene expression changes in both single- and double-knockdown embryos, indicating that transcriptional effects can occur even in the absence of major architectural disruption. Together, our findings demonstrate that Pds5a and Pds5b act cooperatively to constrain cohesin-mediated long-range interactions during embryogenesis and highlight the importance of analyzing cohesin regulator function within a developmental context to understand how 3D genome organization is established in vivo.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 3","pages":"e70054"},"PeriodicalIF":1.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13088002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147700482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Convergence of Cell Biology and Developmental Biology: A Report of the Joint Meeting of the 77th JSCB and the 58th JSDB 细胞生物学与发育生物学的融合：第77届JSCB和第58届JSDB联席会议报告。

IF 1 4区生物学

Development Growth & Differentiation Pub Date : 2026-03-31 DOI: 10.1111/dgd.70052

Asuka C. Kido, Tatsushi Igaki

引用次数: 0

Anisotropic Cellular Forces Drive Hexagonal-to-Tetragonal Tiling Transitions in the Drosophila Eye 各向异性细胞力驱动果蝇眼六边形到四边形的平铺转换。

IF 1 4区生物学

Development Growth & Differentiation Pub Date : 2026-03-10 DOI: 10.1111/dgd.70050

Ting Zheng, Steven R. Davis, Cuicui Li, Weichao Ren, Makoto Sato

{"title":"Anisotropic Cellular Forces Drive Hexagonal-to-Tetragonal Tiling Transitions in the Drosophila Eye","authors":"Ting Zheng, Steven R. Davis, Cuicui Li, Weichao Ren, Makoto Sato","doi":"10.1111/dgd.70050","DOIUrl":"10.1111/dgd.70050","url":null,"abstract":"Tile patterns are fundamental organizational principles of multicellular epithelial tissues. The Drosophila compound eye provides a striking example, in which ommatidia are arranged in a highly regular hexagonal lattice, while tetragonal patterns emerge in specific small-eye mutants. Although increased dorsoventral tension has been implicated in this hexagonal-to-tetragonal transition, conventional vertex models fail to reproduce the observed pattern transformation, indicating the presence of additional uncharacterized force-generating mechanisms. Here, we demonstrate that anisotropic cellular forces driven by radial actin fibers are a key determinant of ommatidial tiling geometry. By extending the vertex model to incorporate both dorsoventral stretching and anisotropic forces that generate rotational torque at cell boundaries, we successfully recapitulate the hexagonal-to-tetragonal transition observed in mutant eyes. Experimental disruption of radial actin fibers suppressed tetragonal pattern formation and induced irregular tiling, providing in vivo support for the model predictions. Importantly, in silico analyses further revealed that anisotropic forces play a dual role: while they drive tetragonalization under symmetry-breaking conditions in mutant eyes, they stabilize regular hexagonal tiling in the wild-type context. These findings identify anisotropic cellular forces as an essential component of epithelial pattern formation and establish an extended vertex model framework for understanding force-driven morphogenetic transitions during development.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"68 3","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13017196/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147391640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0