Development Growth & Differentiation最新文献_第10页

Automated contour extraction for light-sheet microscopy images of zebrafish embryos based on object edge detection algorithm 基于目标边缘检测算法的斑马鱼胚胎光片显微图像轮廓自动提取

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-06-23 DOI: 10.1111/dgd.12871

Akiko Kondow, Kiyoshi Ohnuma, Atsushi Taniguchi, Joe Sakamoto, Makoto Asashima, Kagayaki Kato, Yasuhiro Kamei, Shigenori Nonaka

{"title":"Automated contour extraction for light-sheet microscopy images of zebrafish embryos based on object edge detection algorithm","authors":"Akiko Kondow, Kiyoshi Ohnuma, Atsushi Taniguchi, Joe Sakamoto, Makoto Asashima, Kagayaki Kato, Yasuhiro Kamei, Shigenori Nonaka","doi":"10.1111/dgd.12871","DOIUrl":"10.1111/dgd.12871","url":null,"abstract":"Embryo contour extraction is the initial step in the quantitative analysis of embryo morphology, and it is essential for understanding the developmental process. Recent developments in light-sheet microscopy have enabled the in toto time-lapse imaging of embryos, including zebrafish. However, embryo contour extraction from images generated via light-sheet microscopy is challenging owing to the large amount of data and the variable sizes, shapes, and textures of objects. In this report, we provide a workflow for extracting the contours of zebrafish blastula and gastrula without contour labeling of an embryo. This workflow is based on the edge detection method using a change point detection approach. We assessed the performance of the edge detection method and compared it with widely used edge detection and segmentation methods. The results showed that the edge detection accuracy of the proposed method was superior to those of the Sobel, Laplacian of Gaussian, adaptive threshold, Multi Otsu, and k-means clustering-based methods, and the noise robustness of the proposed method was superior to those of the Multi Otsu and k-means clustering-based methods. The proposed workflow was shown to be useful for automating small-scale contour extractions of zebrafish embryos that cannot be specifically labeled owing to constraints, such as the availability of microscopic channels. This workflow may offer an option for contour extraction when deep learning-based approaches or existing non-deep learning-based methods cannot be applied.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"65 6","pages":"311-320"},"PeriodicalIF":2.5,"publicationDate":"2023-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10411491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Involvement of the scalloped gene in morphogenesis of the wing margin via regulating cell growth in a hemimetabolous insect Gryllus bimaculatus 扇贝基因通过调节半代谢昆虫细胞生长参与翼缘形态发生

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-06-13 DOI: 10.1111/dgd.12869

Takahisa Yamashita, Takahiro Ohde, Taro Nakamura, Yoshiyasu Ishimaru, Takahito Watanabe, Sayuri Tomonari, Yuki Nakamura, Sumihare Noji, Taro Mito

{"title":"Involvement of the scalloped gene in morphogenesis of the wing margin via regulating cell growth in a hemimetabolous insect Gryllus bimaculatus","authors":"Takahisa Yamashita, Takahiro Ohde, Taro Nakamura, Yoshiyasu Ishimaru, Takahito Watanabe, Sayuri Tomonari, Yuki Nakamura, Sumihare Noji, Taro Mito","doi":"10.1111/dgd.12869","DOIUrl":"10.1111/dgd.12869","url":null,"abstract":"The acquisition of wings was a key event in insect evolution. As hemimetabolous insects were the first group to acquire functional wings, establishing the mechanisms of wing formation in this group could provide useful insights into their evolution. In this study, we aimed to elucidate the expression and function of the gene scalloped (sd), which is involved in wing formation in Drosophila melanogaster, and in Gryllus bimaculatus mainly during postembryonic development. Expression analysis showed that sd is expressed in the tergal edge, legs, antennae, labrum, and cerci during embryogenesis and in the distal margin of the wing pads from at least the sixth instar in the mid to late stages. Because sd knockout caused early lethality, nymphal RNA interference experiments were performed. Malformations were observed in the wings, ovipositor, and antennae. By analyzing the effects on wing morphology, it was revealed that sd is mainly involved in the formation of the margin, possibly through the regulation of cell proliferation. In conclusion, sd might regulate the local growth of wing pads and influence wing margin morphology in Gryllus.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"65 6","pages":"348-359"},"PeriodicalIF":2.5,"publicationDate":"2023-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10090927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Daughter cells inherit YAP localization from mother cells in early preimplantation embryos 子细胞在早期着床前胚胎中继承母细胞的YAP定位

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-06-13 DOI: 10.1111/dgd.12870

Tomoaki Otsuka, Hiromi Shimojo, Hiroshi Sasaki

{"title":"Daughter cells inherit YAP localization from mother cells in early preimplantation embryos","authors":"Tomoaki Otsuka, Hiromi Shimojo, Hiroshi Sasaki","doi":"10.1111/dgd.12870","DOIUrl":"10.1111/dgd.12870","url":null,"abstract":"The first stage of cell differentiation during mouse development is the differentiation into the trophectoderm and inner cell mass, which occurs during the 8–32-cell stages of preimplantation embryos. This differentiation is regulated by the Hippo signaling pathway. At the 32-cell stage, embryos establish a position-dependent distribution of the Hippo pathway coactivator, Yes-associated protein 1 (YAP, encoded by Yap1). The outer and inner cells showed nuclear and cytoplasmic localization of YAP, respectively. However, the process by which embryos establish position-dependent YAP localization remains elusive. Here, we established a YAP-reporter mouse line, Yap1mScarlet, and examined YAP–mScarlet protein dynamics during the 8–32-cell stages using live imaging. During mitosis, YAP–mScarlet diffused throughout the cells. YAP–mScarlet dynamics in daughter cells varied depending on the cell division patterns. YAP–mScarlet localization in daughter cells at the completion of cell division coincided with that in mother cells. Experimental manipulation of YAP–mScarlet localization in mother cells also altered its localization in daughter cells upon completion of cell division. In daughter cells, YAP–mScarlet localization gradually changed to the final pattern. In some divisions during the 8–16-cell stages, the cytoplasmic YAP–mScarlet localization preceded cell internalization. These results suggest that cell position is not a primary determinant of YAP localization and that the Hippo signaling status of the mother cell is inherited by the daughter cells, which likely contributes to the stabilization of the cell fate specification process beyond cell division.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"65 6","pages":"360-369"},"PeriodicalIF":2.5,"publicationDate":"2023-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10097198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hormonal and pheromonal studies on amphibians with special reference to metamorphosis and reproductive behavior 两栖动物的激素和信息素研究，特别涉及变态和生殖行为

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-05-29 DOI: 10.1111/dgd.12868

Sakae Kikuyama, Kazutoshi Yamamoto, Fumiyo Toyoda, Tom Kouki, Reiko Okada

{"title":"Hormonal and pheromonal studies on amphibians with special reference to metamorphosis and reproductive behavior","authors":"Sakae Kikuyama, Kazutoshi Yamamoto, Fumiyo Toyoda, Tom Kouki, Reiko Okada","doi":"10.1111/dgd.12868","DOIUrl":"10.1111/dgd.12868","url":null,"abstract":"In this article, we review studies which have been conducted to investigate the hormonal influence on metamorphosis in bullfrog (Rana catesbeiana) and Japanese toad (Bufo japonicus) larvae, in addition to studies conducted on the hormonal and pheromonal control of reproductive behavior in red-bellied newts (Cynops pyrrhogaster). Metamorphosis was studied with an emphasis on the roles of prolactin (PRL) and thyrotropin (TSH). The release of PRL was shown to be regulated by thyrotropin-releasing hormone (TRH) and that of TSH was evidenced to be regulated by corticotropin-releasing factor. The significance of the fact that the neuropeptide that controls the secretion of TSH is different from those encountered in mammals is discussed in consideration of the observation that the release of TRH, which stimulates the release of PRL, is enhanced when the animals are subjected to a cold temperature. Findings that were made by using melanin-rich cells of Bufo embryos and larvae, such as the determination of the origin of the adenohypophyseal primordium, identification of the pancreatic chitinase, and involvement of the rostral preoptic recess organ as the hypothalamic inhibitory center of α-melanocyte-stimulating hormone (α-MSH) secretion, are mentioned in this article. In addition, the involvement of hormones in eliciting courtship behavior in male red-bellied newts and the discovery of the peptide sex pheromones and hormonal control of their secretion are also discussed in the present article.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"65 6","pages":"321-336"},"PeriodicalIF":2.5,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10052593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crosstalk between the mTOR and Hippo pathways mTOR和Hippo通路之间的串扰

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-05-20 DOI: 10.1111/dgd.12867

Daichi Honda, Misako Okumura, Takahiro Chihara

引用次数: 3

Ex vivo expansion of primary cells from limb tissue of Pleurodeles waltl 侧耳侧壁肢组织原代细胞的体外扩增

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-05-20 DOI: 10.1111/dgd.12866

Md. Mahmudul Hasan, Reiko Sekiya, Tao-Sheng Li

{"title":"Ex vivo expansion of primary cells from limb tissue of Pleurodeles waltl","authors":"Md. Mahmudul Hasan, Reiko Sekiya, Tao-Sheng Li","doi":"10.1111/dgd.12866","DOIUrl":"10.1111/dgd.12866","url":null,"abstract":"Pleurodeles waltl is coming to light as a model animal, especially in regeneration studies, but deep studies on the molecular mechanisms have been limited due to the absence of primary tissue cells for wide usage. Therefore, we aimed to grow primary cells from limb tissue of P. waltl for in vitro experiments. Limb tissues were cut into small pieces and seeded as “explants” on culture dishes coated with fibronectin and gelatin. Compared to the control without coating, both fibronectin and gelatin supported quicker outgrowth of cells from explants and faster cell adhesion, and fibronectin showed significantly better performance than gelatin. Interestingly, the doubling time of cells on fibronectin- and gelatin-coated surfaces was almost the same (42.39 ± 2.79 h vs. 42.91 ± 3.69 h) and was not significantly different from that on non-coated plates (49.64 ± 3.63 h). The cryopreserved cells were successfully recovered and showed a multiplication capacity that was similar to that of fresh cells. Senescent cells were barely detected even after long-term sub-culture (>15 passages). Moreover, enhanced fluorescence of MitoSOX™ Red in cells under H2O2 exposure confirmed the respondence to chemical stimuli. Collectively, our results show that we are able to grow enough good-quality cells from P. waltl limb tissue for in vitro experiments, and fibronectin coating provides the best biocompatible environment for cell outgrowth and attachment.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"65 5","pages":"255-265"},"PeriodicalIF":2.5,"publicationDate":"2023-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9649283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mathematical modeling for developmental processes 发展过程的数学建模

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-05-16 DOI: 10.1111/dgd.12856

Yoh Iwasa

{"title":"Mathematical modeling for developmental processes","authors":"Yoh Iwasa","doi":"10.1111/dgd.12856","DOIUrl":"10.1111/dgd.12856","url":null,"abstract":"We review several mathematical models and concepts in developmental biology that have been established over the last decade. (1) Feedback vertex set: Ascidian embryos contain cells of seven types, and cell fate is controlled by ~100 interacting genes. The “feedback vertex set” of the directed graph of the gene regulatory network consists of a small number of genes. By experimentally manipulating them, we can differentiate cells into any cell type. (2) Tissue deformation: Describing morphological changes in tissues and relating them to gene expression and other cellular processes is key in understanding morphogenesis. Expansion and anisotropy of the tissue are described by a “deformation tensor” at each location. A study on chick limb bud formation revealed that both the volume growth rate and anisotropy in deformation differed significantly between locations and stages. (3) Mechanobiology: Forces operating on each cell may alter cell shape and gene expression, which may subsequently exert forces on their surroundings. Measurements of force, tissue shape, and gene expression help us understand autonomous tissue deformation. (4) Adaptive design of development: An optimal growth schedule in fluctuating environments explains the growth response to starvation in Drosophila larvae. Adaptive placement of morphogen sources makes development robust to noises.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"65 5","pages":"272-281"},"PeriodicalIF":2.5,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12856","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9642773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A geometrical perspective on development 发展的几何视角

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-05-16 DOI: 10.1111/dgd.12855

Archishman Raju, Eric D. Siggia

引用次数: 3

Step-by-step protocol for alternative injury models in newt cardiac regeneration 蝾螈心脏再生替代损伤模型的分步方案

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-05-08 DOI: 10.1111/dgd.12854

Hiromi Ikuta, Hitoshi Uemasu, Mitsuki Kyakuno, Yukio Satoh, Noriyuki Namba, Takashi Takeuchi, Toshinori Hayashi

{"title":"Step-by-step protocol for alternative injury models in newt cardiac regeneration","authors":"Hiromi Ikuta, Hitoshi Uemasu, Mitsuki Kyakuno, Yukio Satoh, Noriyuki Namba, Takashi Takeuchi, Toshinori Hayashi","doi":"10.1111/dgd.12854","DOIUrl":"10.1111/dgd.12854","url":null,"abstract":"Although the heart is one of the most important organs for animal survival, its regenerative capacity varies among animal species. Notably, adult mammals cannot regenerate their hearts after damage such as acute myocardial infarction. In contrast, some vertebrate animals can regenerate the heart throughout their lives. Cross-species comparative studies are important to understand the full picture of cardiac regeneration in vertebrates. Among the animal species able to regenerate the heart, some urodele amphibians, such as newts, possess a remarkable capacity for this process. Standardized methods of inducing cardiac regeneration in the newt are needed as a platform for studies comparing newts and other animal models. The procedures presented here describe amputation and cryo-injury techniques for the induction of cardiac regeneration in Pleurodeles waltl, an emerging model newt species. Both procedures consist of simplified steps that require no special equipment. We additionally show some examples of the regenerative process obtained using these procedures. This protocol has been developed for P. waltl. However, these methods are also expected to be applicable to other newt and salamander species, facilitating comparative research with other model animals.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"65 5","pages":"266-271"},"PeriodicalIF":2.5,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10006400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Appetizer on soft matter physics concepts in mechanobiology 力学生物学中软物质物理概念的开胃菜

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-05-01 DOI: 10.1111/dgd.12853

Yuting Lou

引用次数: 0