从体外培养的细胞推导出硬核珊瑚(Acropora tenuis)幼虫细胞的特性。

IF 1.7 4区 生物学 Q4 CELL BIOLOGY
Kaz Kawamura, Satoko Sekida, Koki Nishitsuji, Noriyuki Satoh
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引用次数: 0

摘要

在之前的研究中,我们已经建立了大约15个来自小Acropora tenuis的浮藻幼虫的培养细胞系。根据其形态和行为,将这些细胞分为扁平无定形细胞(FAmCs)、液泡贴壁细胞(vadc)和小光滑细胞(SSmCs)三种类型。famc包括成纤维细胞样细胞和球形亮棕色细胞(BBrCs),它们可以相互转化。为了检验这三种细胞类型的幼虫来源,我们分别培养了抗体:识别FAmC的抗atmlrp2,识别BBrC的抗atahnak,识别SSmC的抗atsomp5和抗atendog,以及识别VAdC的抗atgal和抗atfat4。Anti-AtMLRP2抗体在体外外胚层周围的气孔和神经母细胞样细胞中染色。抗atahnak抗体染色神经元样细胞和神经母细胞样细胞,两者也染色神经元特异性微管蛋白β-3抗体。这些结果表明体外BBrCs和体内神经母细胞样细胞具有共同的神经元特性。两种针对SSmCs的抗体,抗atsomp5和抗atendog染色了幼虫外胚层细胞,表明SSmCs具有幼虫外胚层特性。两种针对vadc的抗体(抗atgal和抗atfat4)可染色幼虫内胚层细胞,表明vadc具有幼虫内胚层特性。因此,体外培养的细胞系似乎保留了造口层、神经母细胞、外胚层或内胚层的特性。它们中的每一种都可以用于未来的研究,以揭示珊瑚幼虫细胞类型的细胞和分子特性,例如共生的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The property of larval cells of the scleractinian coral, Acropora tenuis, deduced from in vitro cultured cells

The property of larval cells of the scleractinian coral, Acropora tenuis, deduced from in vitro cultured cells

In previous studies, we have established approximately 15 cultured cell-lines derived from planula larvae of Acropora tenuis. Based on their morphology and behavior, these cells were classified into three types, flattened amorphous cells (FAmCs), vacuolated adherent cells (VAdCs), and small smooth cells (SSmCs). FAmCs include fibroblast-like cells and spherical, brilliant brown cells (BBrCs), which are transformable to each other. To examine the larval origin of the three cell types, we raised antibodies: anti-AtMLRP2 that appears to recognize FAmC, anti-AtAHNAK for BBrC, anti-AtSOMP5 and anti-AtEndoG for SSmC, and anti-AtGal and anti-AtFat4 for VAdC, respectively. Anti-AtMLRP2 antibody stained in vivo stomodeum and neuroblast-like cells embedded in larval ectoderm around the aboral pole. Anti-AtAHNAK antibody stained neuron-like and neuroblast-like cells, both of which were also stained with neuron-specific tubulin β-3 antibody. These results suggest that in vitro BBrCs and in vivo neuroblast-like cells share neuronal properties in common. Two antibodies for SSmCs, anti-AtSOMP5 and anti-AtEndoG, stained larval ectoderm cells, suggesting that SSmCs have larval ectoderm properties. Two antibodies for VAdCs, anti-AtGal and anti-AtFat4, stained larval endoderm cells, suggesting that VAdCs have larval endoderm properties. Therefore, the in vitro cell lines appear to retain properties of the stomodeum, neuroblast, ectoderm, or endoderm. Each of them may be used in future investigations to reveal cellular and molecular properties of cell types of coral larvae, such as the potential for symbiosis.

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来源期刊
Development Growth & Differentiation
Development Growth & Differentiation 生物-发育生物学
CiteScore
4.60
自引率
4.00%
发文量
62
审稿时长
6 months
期刊介绍: Development Growth & Differentiation (DGD) publishes three types of articles: original, resource, and review papers. Original papers are on any subjects having a context in development, growth, and differentiation processes in animals, plants, and microorganisms, dealing with molecular, genetic, cellular and organismal phenomena including metamorphosis and regeneration, while using experimental, theoretical, and bioinformatic approaches. Papers on other related fields are also welcome, such as stem cell biology, genomics, neuroscience, Evodevo, Ecodevo, and medical science as well as related methodology (new or revised techniques) and bioresources. Resource papers describe a dataset, such as whole genome sequences and expressed sequence tags (ESTs), with some biological insights, which should be valuable for studying the subjects as mentioned above. Submission of review papers is also encouraged, especially those providing a new scope based on the authors’ own study, or a summarization of their study series.
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