Development Growth & Differentiation最新文献_第4页

Correction to “Mosaic gene expression analysis of semaphorin–plexin interactions in Caenorhabditis elegans using the IR-LEGO single-cell gene induction system” 利用IR-LEGO单细胞基因诱导系统对秀丽隐杆线虫中的semaphorin-plexin相互作用进行镶嵌式基因表达分析 "的更正。

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2024-01-17 DOI: 10.1111/dgd.12903

引用次数: 0

Transcription factor Tcf21 modulates urinary bladder size and differentiation 转录因子 Tcf21 可调节膀胱大小和分化。

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2024-01-10 DOI: 10.1111/dgd.12906

Elizabeth A. Mann, Melissa S. Mogle, Joo-Seop Park, Pramod Reddy

引用次数: 0

Independent mesenchymal progenitor pools respectively produce and maintain osteogenic and chondrogenic cells in zebrafish 独立的间充质祖细胞池分别产生并维持斑马鱼的成骨细胞和软骨细胞。

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2024-01-09 DOI: 10.1111/dgd.12908

Hiroaki Komiya, Yuko Sato, Hiroshi Kimura, Atsushi Kawakami

{"title":"Independent mesenchymal progenitor pools respectively produce and maintain osteogenic and chondrogenic cells in zebrafish","authors":"Hiroaki Komiya, Yuko Sato, Hiroshi Kimura, Atsushi Kawakami","doi":"10.1111/dgd.12908","DOIUrl":"10.1111/dgd.12908","url":null,"abstract":"Skeletal tissues including cartilage and bones are characteristic features of vertebrates that are crucial for supporting body morphology and locomotion. Studies mainly in mice have shown that osteoblasts and chondroblasts are supplied from several progenitors like the sclerotome cells in the embryonic stage, osteo-chondroprogenitors in growing long bones, and skeletal stem cells of bone marrow in the postnatal period. However, the exact origins of progenitor cells, their lineage relationships, and their potential to differentiate into osteoblasts and chondroblasts from embryos to adult tissues are not well understood. In this study, we conducted clonal cell tracking in zebrafish and showed that sox9a+ cells are already committed to either chondrogenic or osteogenic fates during embryonic stages and that respective progenies are independently maintained as mesenchymal progenitor pools. Once committed, they never change their lineage identities throughout animal life, even through regeneration. In addition, we further revealed that only osteogenic mesenchymal cells replenish the osteoblast progenitor cells (OPCs), a population of reserved tissue stem cells found to be involved in the de novo production of osteoblasts during regeneration and homeostasis in zebrafish. Thus, our clonal cell tracking study in zebrafish firstly revealed that the mesenchymal progenitor cells that are fated to develop into either chondroblasts or osteoblasts serve as respective tissue stem cells to maintain skeletal tissue homeostasis. Such mesenchymal progenitors dedicated to producing either chondroblasts or osteoblasts would be important targets for skeletal tissue regeneration.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 2","pages":"161-171"},"PeriodicalIF":2.5,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12908","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139405094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene correction and overexpression of TNNI3 improve impaired relaxation in engineered heart tissue model of pediatric restrictive cardiomyopathy 基因校正和过表达 TNNI3 可改善小儿局限性心肌病工程心脏组织模型中受损的松弛功能。

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2024-01-09 DOI: 10.1111/dgd.12909

Moyu Hasegawa, Kenji Miki, Takuji Kawamura, Ikue Takei Sasozaki, Yuki Higashiyama, Masaru Tsuchida, Kunio Kashino, Masaki Taira, Emiko Ito, Maki Takeda, Hidekazu Ishida, Shuichiro Higo, Yasushi Sakata, Shigeru Miyagawa

{"title":"Gene correction and overexpression of TNNI3 improve impaired relaxation in engineered heart tissue model of pediatric restrictive cardiomyopathy","authors":"Moyu Hasegawa, Kenji Miki, Takuji Kawamura, Ikue Takei Sasozaki, Yuki Higashiyama, Masaru Tsuchida, Kunio Kashino, Masaki Taira, Emiko Ito, Maki Takeda, Hidekazu Ishida, Shuichiro Higo, Yasushi Sakata, Shigeru Miyagawa","doi":"10.1111/dgd.12909","DOIUrl":"10.1111/dgd.12909","url":null,"abstract":"Research on cardiomyopathy models using engineered heart tissue (EHT) created from disease-specific induced pluripotent stem cells (iPSCs) is advancing rapidly. However, the study of restrictive cardiomyopathy (RCM), a rare and intractable cardiomyopathy, remains at the experimental stage because there is currently no established method to replicate the hallmark phenotype of RCM, particularly diastolic dysfunction, in vitro. In this study, we generated iPSCs from a patient with early childhood-onset RCM harboring the TNNI3 R170W mutation (R170W-iPSCs). The properties of R170W-iPSC-derived cardiomyocytes (CMs) and EHTs were evaluated and compared with an isogenic iPSC line in which the mutation was corrected. Our results indicated altered calcium kinetics in R170W-iPSC-CMs, including prolonged tau, and an increased ratio of relaxation force to contractile force in R170W-EHTs. These properties were reversed in the isogenic line, suggesting that our model recapitulates impaired relaxation of RCM, i.e., diastolic dysfunction in clinical practice. Furthermore, overexpression of wild-type TNNI3 in R170W-iPSC-CMs and -EHTs effectively rescued impaired relaxation. These results highlight the potential efficacy of EHT, a modality that can accurately recapitulate diastolic dysfunction in vitro, to elucidate the pathophysiology of RCM, as well as the possible benefits of gene therapies for patients with RCM.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 2","pages":"119-132"},"PeriodicalIF":2.5,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12909","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139405093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of progenitor cell survival by a novel chromatin remodeling factor during neural tube development 一种新的染色质重塑因子在神经管发育过程中对祖细胞存活的调控。

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-11-28 DOI: 10.1111/dgd.12905

Noriaki Sasai, Shogo Tada, Jumi Ohshiro, Chikara Kogiso, Takuma Shinozuka

引用次数: 0

Modeling familial and sporadic Parkinson's disease in small fishes 模拟小鱼的家族性和散发性帕金森病。

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-11-22 DOI: 10.1111/dgd.12904

Tomoyuki Yamanaka, Hideaki Matsui

{"title":"Modeling familial and sporadic Parkinson's disease in small fishes","authors":"Tomoyuki Yamanaka, Hideaki Matsui","doi":"10.1111/dgd.12904","DOIUrl":"10.1111/dgd.12904","url":null,"abstract":"The establishment of animal models for Parkinson's disease (PD) has been challenging. Nevertheless, once established, they will serve as valuable tools for elucidating the causes and pathogenesis of PD, as well as for developing new strategies for its treatment. Following the recent discovery of a series of PD causative genes in familial cases, teleost fishes, including zebrafish and medaka, have often been used to establish genetic PD models because of their ease of breeding and gene manipulation, as well as the high conservation of gene orthologs. Some of the fish lines can recapitulate PD phenotypes, which are often more pronounced than those in rodent genetic models. In addition, a new experimental teleost fish, turquoise killifish, can be used as a sporadic PD model, because it spontaneously manifests age-dependent PD phenotypes. Several PD fish models have already made significant contributions to the discovery of novel PD pathological features, such as cytosolic leakage of mitochondrial DNA and pathogenic phosphorylation in α-synuclein. Therefore, utilizing various PD fish models with distinct degenerative phenotypes will be an effective strategy for identifying emerging facets of PD pathogenesis and therapeutic modalities.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 1","pages":"4-20"},"PeriodicalIF":2.5,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138292325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combinatorial genetic strategies for dissecting cell lineages, cell types, and gene function in the mouse brain 在小鼠脑中解剖细胞系、细胞类型和基因功能的组合遗传策略。

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-11-14 DOI: 10.1111/dgd.12902

Qi Zhang, Xue Liu, Ling Gong, Miao He

引用次数: 0

Distribution of XTdrd6/Xtr protein during oogenesis and early development in Xenopus laevis: Zygotic translation begins only in germ cells that have entered the genital ridge XTdrd6/Xtr蛋白在非洲爪蟾卵子发生和早期发育过程中的分布：合子翻译仅在进入生殖嵴的生殖细胞中开始。

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-11-09 DOI: 10.1111/dgd.12899

Tetsuharu Sugimoto, Chihiro Kanayama, Masateru Hiyoshi, Daisuke Kosumi, Kazufumi Takamune

{"title":"Distribution of XTdrd6/Xtr protein during oogenesis and early development in Xenopus laevis: Zygotic translation begins only in germ cells that have entered the genital ridge","authors":"Tetsuharu Sugimoto, Chihiro Kanayama, Masateru Hiyoshi, Daisuke Kosumi, Kazufumi Takamune","doi":"10.1111/dgd.12899","DOIUrl":"10.1111/dgd.12899","url":null,"abstract":"We previously identified Xenopus tudor domain containing 6/Xenopus tudor repeat (Xtdrd6/Xtr), which was exclusively expressed in the germ cells of adult Xenopus laevis. Western blot analysis showed that the XTdrd6/Xtr protein was translated in St. I/II oocytes and persisted as a maternal factor until the tailbud stage. XTdrd6/Xtr has been reported to be essential for the translation of maternal mRNA involved in oocyte meiosis. In the present study, we examined the distribution of the XTdrd6/Xtr protein during oogenesis and early development, to predict the time point of its action during development. First, we showed that XTdrd6/Xtr is localized to germinal granules in the germplasm by electron microscopy. XTdrd6/Xtr was found to be localized to the origin of the germplasm, the mitochondrial cloud of St. I oocytes, during oogenesis. Notably, XTdrd6/Xtr was also found to be localized around the nuclear membrane of St. I oocytes. This suggests that XTdrd6/Xtr may immediately interact with some mRNAs that emerge from the nucleus and translocate to the mitochondrial cloud. XTdrd6/Xtr was also detected in primordial germ cells and germ cells throughout development. Using transgenic Xenopus expressing XTdrd6/Xtr with a C-terminal FLAG tag produced by homology-directed repair, we found that the zygotic translation of the XTdrd6/Xtr protein began at St. 47/48. As germ cells are surrounded by gonadal somatic cells and are considered to enter a new differentiation stage at this phase, the newly synthesized XTdrd6/Xtr protein may regulate the translation of mRNAs involved in the new steps of germ cell differentiation.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 1","pages":"66-74"},"PeriodicalIF":2.5,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72015946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of ancestral gnathostome Gli3 enhancers with activity in mammals 哺乳动物中具有活性的祖先颚体Gli3增强子的鉴定。

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-11-04 DOI: 10.1111/dgd.12901

Shahid Ali, Muhammad Abrar, Irfan Hussain, Fatima Batool, Rabail Zehra Raza, Hizran Khatoon, Matteo Zoia, Axel Visel, Neil H. Shubin, Marco Osterwalder, Amir Ali Abbasi

{"title":"Identification of ancestral gnathostome Gli3 enhancers with activity in mammals","authors":"Shahid Ali, Muhammad Abrar, Irfan Hussain, Fatima Batool, Rabail Zehra Raza, Hizran Khatoon, Matteo Zoia, Axel Visel, Neil H. Shubin, Marco Osterwalder, Amir Ali Abbasi","doi":"10.1111/dgd.12901","DOIUrl":"10.1111/dgd.12901","url":null,"abstract":"Abnormal expression of the transcriptional regulator and hedgehog (Hh) signaling pathway effector Gli3 is known to trigger congenital disease, most frequently affecting the central nervous system (CNS) and the limbs. Accurate delineation of the genomic cis-regulatory landscape controlling Gli3 transcription during embryonic development is critical for the interpretation of noncoding variants associated with congenital defects. Here, we employed a comparative genomic analysis on fish species with a slow rate of molecular evolution to identify seven previously unknown conserved noncoding elements (CNEs) in Gli3 intronic intervals (CNE15–21). Transgenic assays in zebrafish revealed that most of these elements drive activities in Gli3 expressing tissues, predominantly the fins, CNS, and the heart. Intersection of these CNEs with human disease associated SNPs identified CNE15 as a putative mammalian craniofacial enhancer, with conserved activity in vertebrates and potentially affected by mutation associated with human craniofacial morphology. Finally, comparative functional dissection of an appendage-specific CNE conserved in slowly evolving fish (elephant shark), but not in teleost (CNE14/hs1586) indicates co-option of limb specificity from other tissues prior to the divergence of amniotes and lobe-finned fish. These results uncover a novel subset of intronic Gli3 enhancers that arose in the common ancestor of gnathostomes and whose sequence components were likely gradually modified in other species during the process of evolutionary diversification.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"66 1","pages":"75-88"},"PeriodicalIF":2.5,"publicationDate":"2023-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71488433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adult neurogenesis research in China 中国成人神经发生研究。

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-10-29 DOI: 10.1111/dgd.12900

Xing Luo, Mingyue Xu, Weixiang Guo

引用次数: 0