Biophysics最新文献_第8页

The Effects of Cationic Antiseptics on Spectral Characteristics and Electron Transport in Isolated Photosynthetic Complexes of Photosystems I and II 阳离子防腐剂对光系统I和II分离光合配合物光谱特性和电子传递的影响

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S0006350924700519

V. Z. Paschenko, E. P. Lukashev, M. D. Mamedov, D. A. Gvozdev, B. N. Korvatovsky, P. P. Knox, M. G. Strakhovskaya

{"title":"The Effects of Cationic Antiseptics on Spectral Characteristics and Electron Transport in Isolated Photosynthetic Complexes of Photosystems I and II","authors":"V. Z. Paschenko, E. P. Lukashev, M. D. Mamedov, D. A. Gvozdev, B. N. Korvatovsky, P. P. Knox, M. G. Strakhovskaya","doi":"10.1134/S0006350924700519","DOIUrl":"10.1134/S0006350924700519","url":null,"abstract":"<div><p>The effect of cationic antiseptics (at micromolar concentrations) on PSII active core complexes isolated from spinach plants with an intact water oxidation complex and on PSI core complexes from cyanobacterium <i>Synechocystis</i> sp. PCC6803 were studied. Of the antiseptics studied (miramistin, chlorhexidine, octenidine, and picloxydine), octenidine had the greatest effect. It was concluded that its effect on PSII consisted primarily in affecting the structure of the light-harvesting antenna (CP43 and CP47), through which the excitation energy is delivered to the reaction center. As a result, the chlorophyll molecules in this structure were destabilized and their optical and functional characteristics changed. Similar effects were also observed in cyanobacterial complexes of PSI. In addition, the antiseptic affected the rate of establishment of the equilibrium distribution of excited states by spectral forms in the PSI antenna complex. A significant effect of octenidine on the electron transfer rate in the PS I complex was also found: in its presence, the recombination of photo-separated charges between the photoactive pigment P700 and the terminal acceptor F<sub>A</sub>/F<sub>B</sub> occurred twice as fast.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 3","pages":"434 - 444"},"PeriodicalIF":4.033,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142859545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effects of Thorium-232 on the Bioluminescent Enzymatic System and Radioprotective Activity of Humic Substances 钍-232对腐殖质生物发光酶系统及辐射防护活性的影响

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S0006350924700453

T. V. Rozhko, O. V. Kolesnik, A. S. Sachkova, N. Yu. Romanova, D. I. Stom, N. S. Kudryasheva

{"title":"The Effects of Thorium-232 on the Bioluminescent Enzymatic System and Radioprotective Activity of Humic Substances","authors":"T. V. Rozhko, O. V. Kolesnik, A. S. Sachkova, N. Yu. Romanova, D. I. Stom, N. S. Kudryasheva","doi":"10.1134/S0006350924700453","DOIUrl":"10.1134/S0006350924700453","url":null,"abstract":"<div><p>The study of low-dose effects is relevant in connection with the expansion of areas with anthropogenic load. Thorium is one of the background-forming elements in natural ecosystems, its content can increase in the environment during the extraction of natural resources and the operation of thermal power plants. Of particular interest are the bioeffects of thorium in the presence of humic substances, natural detoxifiers. The aim of this study was to evaluate the neutralizing effect of humic substances under conditions of low-dose exposure to thorium-232 (<0.04 Gy). A bioluminescent system of enzymatic reactions, including bacterial luciferase and NAD(P)H:FMN-oxidoreductase, was used as a model biological object. Activation of bioluminescence at the initial stage of thorium-232 exposure (up to 50 min) and an increase in the content of reactive oxygen species after 50 min of exposure were observed. The relationship between the intensity of bioluminescence and the content of reactive oxygen species (correlation coefficient of –0.86) was shown. The presence of humic substances neutralized the activating effect of thorium and reduced the content of reactive oxygen species to a control level. The effect of humic substances on the rate of the NADH-dependent enzymatic redox process has been demonstrated. The results we obtained contribute to the understanding of the molecular mechanisms of thorium-232 exposure and the neutralization of its effect on enzymatic processes.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 3","pages":"370 - 379"},"PeriodicalIF":4.033,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142859782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acclimation of Primary Photosynthetic Reactions in the Cells of Chlamydomonas reinhardtii to Cadmium: Analysis of Cell Population Heterogeneity 莱茵衣藻细胞初级光合反应对镉的驯化：细胞群体异质性分析

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S0006350924700489

A. A. Volgusheva, I. V. Konyukhov, T. K. Antal

{"title":"Acclimation of Primary Photosynthetic Reactions in the Cells of Chlamydomonas reinhardtii to Cadmium: Analysis of Cell Population Heterogeneity","authors":"A. A. Volgusheva, I. V. Konyukhov, T. K. Antal","doi":"10.1134/S0006350924700489","DOIUrl":"10.1134/S0006350924700489","url":null,"abstract":"<div><p>The mechanisms of acclimation of primary photosynthetic reactions in the green microalgae <i>Chlamydomonas reinhardtii</i> to the toxic action of cadmium were investigated by analyzing the dynamics of the distribution of key photosynthetic parameters in the cell population. A synchronous culture of microalgae was incubated for 96 h in the presence of 25 μM Cd, and OJIP-transient curves of individual cells were recorded at different stages of incubation with the toxicant using an original microfluorometer. The analysis of OJIP-transient curves made it possible to determine distributions of key JIP-test parameters: <i>F</i><sub>V</sub>/<i>F</i><sub>M</sub>, <i>ET</i><sub>O</sub>/<i>ABS</i>, <i>RC</i>/<i>ABS</i>, which represent photochemical activity of PS II, electron transport in PS II, and light absorption per active reaction center in PS II, respectively. Acclimation of primary photosynthetic reactions in microalgae to cadmium was accompanied by the appearance of two dominating cell fractions, which demonstrated the stable values of photosynthetic parameters when energy imbalance occurred due to the presence of the toxicant. The cells related to the first fraction retained moderate PS II activity by the effect of reduced PS II antenna size, while the cells of the second fraction showed low photochemical activity of PS II keeping antenna size similar to that of the control cells.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 3","pages":"401 - 407"},"PeriodicalIF":4.033,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142859547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of the Component Composition of Saliva on the Orientation of Two-Enzyme Bioluminescent Analysis Depending on the Type of Physical Load 不同物理负荷下唾液组分对双酶生物发光分析取向的影响

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S0006350924700635

V. V. Malysheva, L. V. Stepanova, A. M. Vyshedko, L. V. Bel’skaya, E. A. Sarf, Z. Khalzhanova, O. A. Kolenchukova, V. A. Kratasyuk

{"title":"The Effect of the Component Composition of Saliva on the Orientation of Two-Enzyme Bioluminescent Analysis Depending on the Type of Physical Load","authors":"V. V. Malysheva, L. V. Stepanova, A. M. Vyshedko, L. V. Bel’skaya, E. A. Sarf, Z. Khalzhanova, O. A. Kolenchukova, V. A. Kratasyuk","doi":"10.1134/S0006350924700635","DOIUrl":"10.1134/S0006350924700635","url":null,"abstract":"<div><p><b>Abstract</b>—The saliva of athletes with anaerobic (track and field athletes) and aerobic (swimmers) physical loads was studied. Saliva was collected before (in a state of physical rest) and after training. It was found that physical load significantly increased the concentration of total protein in saliva and the activity of hydrogen ions (pH) in the saliva. Catalase activity in saliva was significantly increased only after aerobic physical load, as opposed to anaerobic load. The bioluminescent saliva index was significantly increased for athletes engaged in aerobic physical load compared to that for an anaerobic load. Saliva biomarkers such as the concentration of lactate and total protein, catalase activity, concentration of calcium, potassium, and magnesium ions, as well as the content of group IV amides, had a significant effect on the enhancement of light emission in a bioluminescent two-enzyme reaction. The content of group IV amides and a complex of oligosaccharides, polysaccharides, phosphatase, and phospholipids in the saliva of athletes engaged in anaerobic physical load could more strongly inhibit bioluminescent glow than the concentration of triene conjugates and the content of group II amides in the saliva of athletes engaged in aerobic physical load. Thus, significant biomarkers of the physiological state of the body have been identified in the saliva of athletes engaged in aerobic or anaerobic physical loads, which were able to affect the integral response of a bioluminescent enzymatic biotest. A change in the integral bioluminescent saliva index, depending on the type of physical load for an athlete, can be used in sports medicine to prevent physical overloads.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 3","pages":"566 - 574"},"PeriodicalIF":4.033,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142859642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photobiology in Russia 俄罗斯的光生物学

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S0006350924700659

A. A. Tsygankov, M. M. Borisova-Mubarakshina, E. S. Vysotsky, A. E. Solovchenko, N. V. Suvorov, V. V. Tuchin

引用次数: 0

Assessment of Antenna Heterogeneity and the Activity of the Oxygen-Evolving Complex of Photosystem II Using Mathematical Methods 用数学方法评估天线异质性和光系统 II 氧发生复合物的活性

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S0006350924700490

N. S. Degtereva, T. Yu. Plyusnina, S. S. Khrushchev, R. N. Chervitsov, E. N. Voronova, O. V. Yakovleva, T. K. Antal, G. Yu. Riznichenko, A. B. Rubin

{"title":"Assessment of Antenna Heterogeneity and the Activity of the Oxygen-Evolving Complex of Photosystem II Using Mathematical Methods","authors":"N. S. Degtereva, T. Yu. Plyusnina, S. S. Khrushchev, R. N. Chervitsov, E. N. Voronova, O. V. Yakovleva, T. K. Antal, G. Yu. Riznichenko, A. B. Rubin","doi":"10.1134/S0006350924700490","DOIUrl":"10.1134/S0006350924700490","url":null,"abstract":"<div><p>Photosystem II is one of the main pigment–protein complexes of photosynthesis, which is highly sensitive to adverse environmental factors. The heterogeneity of photosystem II properties is necessary for the resistance of autotrophic organisms to stress factors. The assessment of photosystem II heterogeneity can be used in environmental monitoring for the rapid detection of environmental pollution. A comprehensive approach to the assessment of photosystem II heterogeneity is proposed; it is based on a mathematical analysis of the shape of the chlorophyll <i>a</i> fluorescence induction curve of samples treated with 3-(3,4-dichlorophenyl)-1,1-dimethyl urea using a mathematical model and JIP test parameters. Fluorescence induction curves obtained for treated samples of <i>Chlorella</i>, <i>Scenedesmus</i>, <i>Ankistrodesmus</i>, <i>Pleurochloris</i>, and <i>Stichococcus</i> cell cultures were analyzed that grew in light with an intensity of 8 and 16 W/m<sup>–2</sup>. For all cases, the ratio of reaction centers with different antenna sizes (alpha and beta centers) was estimated and the proportion of active and inactive oxygen-evolving complexes was determined.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 3","pages":"408 - 417"},"PeriodicalIF":4.033,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142859733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Viscous Media on the Quantum Yield of Bioluminescence in a Reaction Catalyzed by Bacterial Luciferase 粘性介质对细菌荧光素酶催化反应中生物发光量子产率的影响

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S0006350924700465

A. E. Lisitsa, L. A. Sukovatyi, V. A. Kratasyuk, E. V. Nemtseva

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A Bioluminescent Test System Based on Recombinant L. mingrelica Firefly Luciferase as a Means of Investigating the Efficacy of the Gentamicin Effect on Live E. coli Cells 基于重组l.m mingrelica萤火虫荧光素酶的生物荧光检测系统研究庆大霉素对活大肠杆菌细胞的作用

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S0006350924700556

G. Yu. Lomakina, S. S. Kaminskaya, N. N. Ugarova

引用次数: 0

Photodynamic and Plasmonic Photothermal Combination Therapy of Transplanted Tumors of a Rat Model 光动力与等离子体光热联合治疗大鼠移植瘤模型

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S0006350924700623

A. B. Bucharskaya, N. A. Navolokin, D. A. Mudrak, G. N. Maslyakova, B. N. Khlebtsov, N. G. Khlebtsov, V. D. Genin, E. A. Genina, V. V. Tuchin

{"title":"Photodynamic and Plasmonic Photothermal Combination Therapy of Transplanted Tumors of a Rat Model","authors":"A. B. Bucharskaya, N. A. Navolokin, D. A. Mudrak, G. N. Maslyakova, B. N. Khlebtsov, N. G. Khlebtsov, V. D. Genin, E. A. Genina, V. V. Tuchin","doi":"10.1134/S0006350924700623","DOIUrl":"10.1134/S0006350924700623","url":null,"abstract":"<div><p>The aim of this study was to develop a combined technology of plasmon photothermal therapy and photodynamic therapy of transplanted cholangiocarcinoma PC-1 in rats. For photodynamic therapy, rats were intratumorally administered with indocyanine green diluted in polyethylene glycol at a ratio of 1 : 100 at a dose of 2 mg/kg. For plasmon photothermal therapy, gold nanorods diluted in polyethylene glycol (400 μg/mL) were intratumorally injected in a volume of 30% of the tumor volume. At 1 h after injection, the tumor was percutaneously irradiated with a diode infrared laser with a wavelength of 808 nm at a power density of 2.3 W/cm<sup>2</sup> for 15 min. The animals were removed from the experiment 72 h and 21 days after therapy. Morphological studies of the tumor were performed on sections stained with standard and immunohistochemical methods. A significant increase in tumor temperature was noted, up to 60.0 ± 4.1°C with combined plasmon photothermal and photodynamic therapy. After 72 h, pronounced necrotic changes were observed in the tumor tissue and intact tumor cells were observed only on the periphery of the tumor. At 21 days after therapy, a significant inhibition of tumor growth was noted; the inhibition index by tumor mass was 77.4%.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 3","pages":"559 - 565"},"PeriodicalIF":4.033,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142859639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Underwater Measurements of Transmitted Light Spectra in Stratified Reservoirs of the White Sea Coast as a Key to the Understanding of Pigment Composition of Phototrophs in the Chemocline Zone 水下测量白海沿岸分层水库的透射光光谱是了解化合层区光养生物色素组成的关键

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S000635092470060X

E. A. Labunskaya, D. A. Voronov, V. I. Lobyshev, E. D. Krasnova

{"title":"Underwater Measurements of Transmitted Light Spectra in Stratified Reservoirs of the White Sea Coast as a Key to the Understanding of Pigment Composition of Phototrophs in the Chemocline Zone","authors":"E. A. Labunskaya, D. A. Voronov, V. I. Lobyshev, E. D. Krasnova","doi":"10.1134/S000635092470060X","DOIUrl":"10.1134/S000635092470060X","url":null,"abstract":"<div><p>Measurements of the spectral composition of light at the boundary of the photic zone in seven coastal reservoirs, which are isolated from the sea to varying degrees, as well as in the marine water area and in a freshwater lake showed that in marine and brackish reservoirs, mainly green light reaches it; in lakes with a fresh upper layer it is orange, red and far-red. In meromictic reservoirs, the photic zone was limited by a colored layer of water with a massive development of phototrophic microorganisms. Their pigment composition is in good agreement with the spectral composition of transmitted light. The marine bays and lagoons were dominated by taxa with red pigments: cryptophytic algae with phycoerythrin-545, purple sulfur bacteria with okenone carotenoid, brown green sulfur bacteria with isorenieratene and bacteriochlorophyll <i>e</i>. In lakes with fresh upper water, unicellular algae and green sulfur bacteria with chlorobactene and bacteriochlorophyll <i>d</i> developed. The spectral range can serve as a selective factor that determines the composition of a community of phototrophs with structurally different antennas and similar light absorption spectra.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 3","pages":"533 - 550"},"PeriodicalIF":4.033,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142859644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0