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Pharmacological Blockade of High Threshold L-Type Calcium Channels in Cardiomyocytes Restores Respiratory Chain Activity Impaired by Vibration 药理阻断心肌细胞中的高阈值 L 型钙通道可恢复受振动影响的呼吸链活动
IF 4.033
Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700301
V. V. Vorobieva, O. S. Levchenkova, P. D. Shabanov
{"title":"Pharmacological Blockade of High Threshold L-Type Calcium Channels in Cardiomyocytes Restores Respiratory Chain Activity Impaired by Vibration","authors":"V. V. Vorobieva,&nbsp;O. S. Levchenkova,&nbsp;P. D. Shabanov","doi":"10.1134/S0006350924700301","DOIUrl":"10.1134/S0006350924700301","url":null,"abstract":"<div><p>The transfer of calcium ions through the cell membrane of a cardiomyocyte is associated with overcoming a high energy barrier and can be impaired by vibration. Since drugs of the calcium channel blocker group are able to reduce damage to mitochondria and cell membranes, the effect of the calcium channel blocker nifedipine (adalat) at a dose of 7.5 mg/kg on the energy metabolism of cardiomyocytes in a vibration-mediated (56 sessions of vibration with a frequency of 44 Hz and an amplitude of 0.5 mm) model of cellular hypoxia was evaluated. The functional states of mitochondria in the composition of 30% homogenate of the myocardial tissue of the left ventricle of the rabbit heart were studied using the polarographic method with a closed galvanic oxygen sensor in a thermostatically controlled 1 mL cuvette with a protein content of 2.4 ± 0.5 mg, measured by a modified microbiuretic assay. In animals subjected to vibration in the presence of calcium channel blockers, the rate of endogenous respiration (<i>V</i><sub>e</sub>) remained at the level of intact animals and was 16.4 ± 4.1 [ng-atom O] min<sup>–1</sup> mg<sup>–1</sup> protein; sensitivity to amytal increased by 39% and sensitivity to malonate decreased by 40% compared with the indicators in animals subjected to vibration without pharmacological protection. The indicators of the ratio of the activity of mitochondrial enzyme complexes I and II and the processes of regulation in different metabolic states of mitochondria confirmed the restoration of the activity of mitochondrial enzyme complex I suppressed by vibration and the regulatory restriction of mitochondrial enzyme complex II. The revealed energy-protective effect of calcium channel blockers increased the vibration resistance of the tissue, preventing the development of necrotic changes in it.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"261 - 266"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Effect of Viral Lysis on Light Absorption by the Black Sea Microalgae Tetraselmis viridis 病毒溶解对黑海微藻 Tetraselmis viridis 光吸收的影响
IF 4.033
Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700313
S. A. Sholar, O. A. Stepanova
{"title":"The Effect of Viral Lysis on Light Absorption by the Black Sea Microalgae Tetraselmis viridis","authors":"S. A. Sholar,&nbsp;O. A. Stepanova","doi":"10.1134/S0006350924700313","DOIUrl":"10.1134/S0006350924700313","url":null,"abstract":"<div><p>Using spectrophotometry, the effect of viral lysis on light absorption in a virus-infected culture of microalgae <i>Tetraselmis viridis</i> was studied compared with the growth of a control uninfected culture. Some methodological issues of conducting multi-day experiments have been worked out, considering the natural titers of algal viruses, equal volumes of composite microbiota (algal viruses, nutrient medium, and microalgae cultures), conditions (temperature and illumination) in the experiment and control with the prevention of the possibility of viral contamination of microalgae in the control. It was found in the dynamics of experiments that during destruction (lysis) of hosts (microalgae cells) by viruses there was an increase in the amount of dissolved organic matter.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"267 - 271"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Relationship between the Temperature in the Deep Layers of the Somatosensory Cortex and Blood Flow Velocity in the Brain of Anesthetized Mice 躯体感觉皮层深层温度与麻醉小鼠大脑血流速度之间的关系
IF 4.033
Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700374
A. M. Romshin, A. A. Osypov, V. K. Krohaleva, S. G. Zhuravlev, O. N. Egorova, I. I. Vlasov, I. Yu. Popova
{"title":"The Relationship between the Temperature in the Deep Layers of the Somatosensory Cortex and Blood Flow Velocity in the Brain of Anesthetized Mice","authors":"A. M. Romshin,&nbsp;A. A. Osypov,&nbsp;V. K. Krohaleva,&nbsp;S. G. Zhuravlev,&nbsp;O. N. Egorova,&nbsp;I. I. Vlasov,&nbsp;I. Yu. Popova","doi":"10.1134/S0006350924700374","DOIUrl":"10.1134/S0006350924700374","url":null,"abstract":"<div><p>Despite the obvious importance of temperature for brain functioning, it remains one of the least studied parameters of the brain. The temperature is determined by the balance between the release of heat during metabolism and its removal by the bloodstream, which is regulated by neurovascular coupling. Using the method of laser speckle contrast imaging and micro-diamond thermometry, we performed a long-term simultaneous recording of blood flow velocity and temperature in the brain of anesthetized animals <i>in vivo</i> for the first time. To study the coupling between temperature and blood flow two approaches were used: enhancing the general blood flow by intraperitoneal injection of adrenaline and enhancing neuronal activity by applying KCl solution to the brain surface. The data we obtained indicated that the temperature of the nerve tissue had a more complex dynamics compared to blood flow, which was apparently associated with direct or indirect activation of both individual neurons and neural ensembles. Studies of temperature dynamics can make a significant contribution to understanding the nature of neurovascular coupling.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"306 - 313"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
On the Role of Priming in the Development of Modern Rehabilitation Technologies 论引导在现代康复技术发展中的作用
IF 4.033
Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700428
A. I. Fedotchev
{"title":"On the Role of Priming in the Development of Modern Rehabilitation Technologies","authors":"A. I. Fedotchev","doi":"10.1134/S0006350924700428","DOIUrl":"10.1134/S0006350924700428","url":null,"abstract":"<div><p>Recent studies using priming, the effect of precedence, in the restoration of impaired functions and cognitive rehabilitation of neurological clinic patients have been analyzed. Various types of priming were considered, including transcranial magnetic and electrical stimulation, as well as the preliminary presentation of acoustic and visual stimuli. The presented data show that the range of conditions and specific types of successful application of priming in the clinic is quite wide, and the number of studies increases annually. It is believed that the activation of neuroplasticity mechanisms underlies the positive effects of priming in the treatment of many neurological and psychogenic disorders. Using the example of the author’s own research, the advantages of visual priming in the form of resonance scanning and LED photostimulation with a gradually increasing frequency within the basic rhythms of the electroencephalogram are emphasized.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"345 - 348"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biophysical Methods for Testing Nanomaterials for Biomedical and Ecotoxicological Purposes Using Daphnia magna as a Model Organism 以大型蚤为模式生物测试用于生物医学和生态毒理学目的的纳米材料的生物物理方法
IF 4.033
Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700386
N. B. Savina, D. V. Uskalova, D. T. Petrosova, E. I. Sarapultseva
{"title":"Biophysical Methods for Testing Nanomaterials for Biomedical and Ecotoxicological Purposes Using Daphnia magna as a Model Organism","authors":"N. B. Savina,&nbsp;D. V. Uskalova,&nbsp;D. T. Petrosova,&nbsp;E. I. Sarapultseva","doi":"10.1134/S0006350924700386","DOIUrl":"10.1134/S0006350924700386","url":null,"abstract":"<div><p>A review of image visualization methods and other biophysical approaches that demonstrate the validity of using higher invertebrates from the crustacean subtype <i>Daphnia magna</i> as a test model for biomedical and ecotoxicological studies is presented. The main characteristics of animals are described, such as a transparent body, small size, filter type of nutrition, and the presence of well-formed organs, as well as a short life cycle, high fertility and sensitivity to toxicants, which allow the use of this test organism in <i>in vivo</i> experiments as a model alternative to warm-blooded animals. The publications are reviewed in which <i>D. magna</i> was used as a model for visualizing the accumulation and distribution of nanomaterials in the body, as well as for analyzing the mechanisms of cytotoxicity. The methods of fluorescence imaging, interference microscopy and spectrophotometry are described.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"314 - 319"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ambiol Modulates the Functional Characteristics of Pea Seedlings Mitochondria 安倍醇调节豌豆幼苗线粒体的功能特征
IF 4.033
Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700295
I. V. Zhigacheva, N. I. Krikunova, Yu. V. Kuznetsov, A. N. Goloshchapov
{"title":"Ambiol Modulates the Functional Characteristics of Pea Seedlings Mitochondria","authors":"I. V. Zhigacheva,&nbsp;N. I. Krikunova,&nbsp;Yu. V. Kuznetsov,&nbsp;A. N. Goloshchapov","doi":"10.1134/S0006350924700295","DOIUrl":"10.1134/S0006350924700295","url":null,"abstract":"<div><p>The effect of the plant growth regulator ambiol on the functional state of mitochondria of etiolated pea seedlings was studied. The drug prevented the activation of lipid peroxidation in mitochondrial membranes in the concentration range of 10<sup>–5</sup>–10<sup>–6</sup> and 10<sup>–9</sup> M. The introduction of ambiol into the incubation medium of these organelles resulted in an increase in the oxidation rates of NAD-dependent substrates. The most effective concentrations were 10<sup>–5</sup> and 10<sup>–6</sup> M. Changes in the bioenergetic characteristics of mitochondria were accompanied by changes in the fatty acid composition of mitochondrial membranes. The most significant changes occurred in the content of 18:2ω6 and 18:3ω3 fatty acids. It was hypothesized that an increase in the content of these fatty acids in the membranes of mitochondria indicated an increase in the content of cardiolipin, which ensured the effective functioning of the mitochondrial respiratory chain and, consequently, the maintenance of energy metabolism at a high level. The treatment of pea seeds with ambiol not only prevented a decrease in the growth rate of pea seedlings under conditions of water stress, but also stimulated their growth.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"231 - 236"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Combined Effects of Electrochemical and Ultrasonic Treatment for Water Purification from Pathogenic Contaminants Based on the Example of Escherichia coli 以大肠埃希氏菌为例，探讨电化学和超声波处理对净化水中致病污染物的联合作用
IF 4.033
Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700325
S. B. Bibikov, A. I. Sergeev, I. I. Barashkova, M. V. Motyakin
{"title":"The Combined Effects of Electrochemical and Ultrasonic Treatment for Water Purification from Pathogenic Contaminants Based on the Example of Escherichia coli","authors":"S. B. Bibikov,&nbsp;A. I. Sergeev,&nbsp;I. I. Barashkova,&nbsp;M. V. Motyakin","doi":"10.1134/S0006350924700325","DOIUrl":"10.1134/S0006350924700325","url":null,"abstract":"<div><p>The combined effects of electrolysis and ultrasound on the population of <i>E. coli</i> bacteria in an aqueous solution of sodium sulfate have been studied. The kinetics of bacterial inactivation with this method for water purification have been determined. It has been shown that the combined effect of ultrasonic and electrochemical treatment of an aqueous solution significantly increased the rate of bacterial inactivation. It has been suggested that hydroxyl radicals formed during combined treatment are responsible for the death of bacteria. A correlation has been found between the rate of formation of hydroxyl radicals and the rate of inactivation of bacteria.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"272 - 277"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Relationship between the Composition of Natural Water and State Lipid Peroxidation in Biological Objects 天然水成分与生物体内脂质过氧化状态之间的关系
IF 4.033
Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700350
L. N. Shishkina, A. S. Dubovik, V. O. Shvydkiy, M. V. Kozlov, E. V. Shtamm, A. G. Georgiadi
{"title":"The Relationship between the Composition of Natural Water and State Lipid Peroxidation in Biological Objects","authors":"L. N. Shishkina,&nbsp;A. S. Dubovik,&nbsp;V. O. Shvydkiy,&nbsp;M. V. Kozlov,&nbsp;E. V. Shtamm,&nbsp;A. G. Georgiadi","doi":"10.1134/S0006350924700350","DOIUrl":"10.1134/S0006350924700350","url":null,"abstract":"<div><p>The relationship between the compositions of five natural water samples and the parameters of the lipid peroxidation regulation in a model system based on natural phospholipids, as well as their participation in the toxicity of natural water, have been studied. It has been shown that the presence of N- and P-containing compounds in natural water samples led to inhibition of lecithin autooxidation and luminescence intensity of luminous bacteria; it had a significant effect on spontaneous aggregation of lecithin and an increase in the content of hydrophobic compounds increased the negative value of the ζ potential of its particles. The high sensitivity of the parameters of the lipid peroxidation regulation system to the composition of natural water, even at low concentrations of its components, allows them to be considered as promising indicators for assessing the effect of natural water on biological objects. Mathematical processing of ultraviolet spectra of natural water samples using the Gauss method can be used as an express test for the analysis of its hydrochemical composition. The effect of natural water components on the state of membranes and intracellular processes of biological objects has been confirmed by biotesting methods.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"292 - 298"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An X-Ray Diffraction Study of Lipid Films with ICHPHAN 用 ICHPHAN 对脂质薄膜进行 X 射线衍射研究
IF 4.033
Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700258
A. V. Krivandin, A. N. Goloshchapov
{"title":"An X-Ray Diffraction Study of Lipid Films with ICHPHAN","authors":"A. V. Krivandin,&nbsp;A. N. Goloshchapov","doi":"10.1134/S0006350924700258","DOIUrl":"10.1134/S0006350924700258","url":null,"abstract":"<div><p>X-ray diffraction was used to study oriented films formed from solutions in chloroform of egg phosphatidylcholine, the synthetic hybrid antioxidant ICHPHAN-10-C-10, and their mixtures containing 12.5 and 32 wt % of ICHPHAN. The air-dried films obtained from phosphatidylcholine and ICHPHAN were multiphase and, in addition to nonlayered lipid phases, contained the crystalline phase of ICHPHAN. With an increase in the degree of hydration of such films, the formation of a single lamellar phase consisting of lipids and ICHPHAN occurred in them. The appearance and disappearance of the crystalline phase of ICHPHAN in the films with a change in their degree of hydration was reversible. The electron density profiles of lipid membranes calculated with a resolution of ~1.2 nm showed that in the presence of ICHPHAN, the membrane thickness was reduced and the ordering of lipid hydrocarbon chains in it decreased. The results we obtained indicated the ability of ICHPHAN to integrate from the crystalline phase into lipid membranes in large quantities.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"225 - 230"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gold and Silver Compounds as Potential Antitumor Drugs 作为潜在抗肿瘤药物的金银化合物
IF 4.033
Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700416
L. A. Ostrovskaya, D. B. Korman, E. I. Nekrasova, A. K. Chigasova, N. V. Bluhterova, V. A. Rikova, M. M. Fomina, Yu. A. Khochenkova, K. A. Abzaeva
{"title":"Gold and Silver Compounds as Potential Antitumor Drugs","authors":"L. A. Ostrovskaya,&nbsp;D. B. Korman,&nbsp;E. I. Nekrasova,&nbsp;A. K. Chigasova,&nbsp;N. V. Bluhterova,&nbsp;V. A. Rikova,&nbsp;M. M. Fomina,&nbsp;Yu. A. Khochenkova,&nbsp;K. A. Abzaeva","doi":"10.1134/S0006350924700416","DOIUrl":"10.1134/S0006350924700416","url":null,"abstract":"<div><p>The generalized results of the study of antitumor activity, cytotoxic effect, and mechanism of action of gold (aurumacryl) and silver (argacryl) polyacrylates are presented in the context of modern ideas about the prospects for the possible use of gold- and silver-containing compounds as potential antitumor drugs based on their experimental study.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"333 - 344"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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