Biophysics最新文献_第9页

CRISPR/Cas System Photocontrolled at the Guide RNA Level CRISPR/Cas 系统在引导 RNA 水平上进行光控

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S000635092470043X

L. V. Sakovina, E. S. Gorlenko, D. S. Novopashina

引用次数: 0

Photochemical Energy Conversion of Far-Red Light in Photosystem I Reaction Centers from Cyanobacterium Acaryochloris marina 蓝藻Acaryochloris marina光系统I反应中心的远红光光化学能量转换

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S0006350924700507

A. A. Petrova, A. P. Casazza, S. Santabarbara, D. A. Cherepanov

{"title":"Photochemical Energy Conversion of Far-Red Light in Photosystem I Reaction Centers from Cyanobacterium Acaryochloris marina","authors":"A. A. Petrova, A. P. Casazza, S. Santabarbara, D. A. Cherepanov","doi":"10.1134/S0006350924700507","DOIUrl":"10.1134/S0006350924700507","url":null,"abstract":"<div>The conversion of light energy in the near-infrared spectral range by photosynthetic pigment–protein complexes has been intensively studied in recent years in connection with the discovery of cyanobacteria, in the photosynthetic apparatus of which chlorophylls f and d absorbing long-wavelength light are present. Acaryochloris marina occupies a special place among such cyanobacteria. Its photosystem I contains mainly chlorophyll d; it is a part of a special P740 pair, the absorption spectrum of which is shifted to the red region by 40 nm. This causes a decrease in the energy of the excited state of P740 by ~0.1 eV compared with photosystem I containing chlorophyll a. Complexes of photosystem I from A. marina also have the following peculiarities: four chlorophyll a molecules of the reaction center are replaced by chlorophyll d, and the third pair of chlorophyll a molecules involved in electron transfer are replaced by pheophytin a. The presence of spectrally different cofactors (chlorophyll d and pheophytin a) made it possible to reliably establish the intermediates of primary charge separation reactions in this photosystem I. In this review, we considered the latest results of studies of photochemical energy conversion in the reaction centers of photosystem I of A. marina and possible mechanisms for compensating energy losses of the use of low-energy far-red light for photosynthesis.</div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 3","pages":"418 - 433"},"PeriodicalIF":4.033,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142859546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effects of Caffeic Acid, Hispidin and a Discovered Activating Component on Luminescence of Mycelium and a Luminescent System of the Basidiomycete Neonothopanus nambi 咖啡酸、Hispidin和一种已发现的活化成分对新南白担子菌菌丝发光及发光系统的影响

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S0006350924700544

N. O. Ronzhin, E. D. Posokhina, V. M. Le, O. A. Mogilnaya, Yu. V. Zakharova, A. S. Sukhikh, V. S. Bondar

{"title":"The Effects of Caffeic Acid, Hispidin and a Discovered Activating Component on Luminescence of Mycelium and a Luminescent System of the Basidiomycete Neonothopanus nambi","authors":"N. O. Ronzhin, E. D. Posokhina, V. M. Le, O. A. Mogilnaya, Yu. V. Zakharova, A. S. Sukhikh, V. S. Bondar","doi":"10.1134/S0006350924700544","DOIUrl":"10.1134/S0006350924700544","url":null,"abstract":"<div>It was found in vivo that the addition of caffeic acid and a low-molecular-weight bioluminescence activator that we have discovered to the mycelium of the luminous fungus Neonothopanus nambi led to a rapid and significant (by an order of magnitude and more) increase in the intensity of its light emission. It is proposed that the observed effect of activation of the fungus glow may be mediated by the oxidation of added substances by enzymes of the ligninolytic complex of basidiomycetes (in particular, peroxidases) with the emission of visible light quanta. Parallel in vivo experiments have shown that the addition of hispidin (a precursor of luciferin in luminous higher fungi) did not affect the intensity of light emission of the mycelium. In vitro studies have shown that caffeic acid and the discovered low-molecular-weight glow activator did not affect the light emission level of the enzyme luminescent system isolated from the mycelium of N. nambi in the presence of NADPH and significantly suppressed the luminescent reaction of the system induced by NADPH and hispidin. The totality of the data we obtained indicated the presence in luminous higher fungi of different biochemical pathways for the generation of visible light quanta involving different enzymes (or enzyme systems) and different substrates.</div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 3","pages":"470 - 476"},"PeriodicalIF":4.033,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142859598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Study of the Effects of Physico-Chemical Factors on the Frequency of Plasmid Transduction by Bacteriophage RB49 理化因素对噬菌体RB49质粒转导频率影响的研究

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S0006350924700532

A. N. Nikulina, N. A. Nikulin, A. A. Zimin

{"title":"A Study of the Effects of Physico-Chemical Factors on the Frequency of Plasmid Transduction by Bacteriophage RB49","authors":"A. N. Nikulina, N. A. Nikulin, A. A. Zimin","doi":"10.1134/S0006350924700532","DOIUrl":"10.1134/S0006350924700532","url":null,"abstract":"<div>T4-related bacteriophage RB49 is capable of general plasmid transduction with a relatively high frequency. Due to this mechanism, bacteria can acquire the ability to adapt to changing environmental conditions and explore new ecological niches. The effects of pH, temperature, and irradiation with long-wavelength ultraviolet light (λ = 366 nm) on the characteristics of the phage RB49 preparation containing transducing particles with pTurboGFP-B plasmid DNA and virulent particles with their own DNA were studied. Data on changes in the titer of virulent particles and the frequency of transduction of the pTurboGFP-B plasmid by the RB49 phage were obtained. After exposure to UV radiation for 2 h, the frequency of transduction of the pTurboGFP-B plasmid by the RB49 phage increased by ~3 times. After storing the preparation in ice for 40 min, a several-fold increase in the transduction frequency was observed. Based on the experimental data we obtained, it was assumed that the transducing particles of the RB49 phage may be more resistant to long-wavelength UV radiation and temperatures close to 0°C than virulent ones and provide the transduction process more efficiently than under normal conditions. Similar processes can occur in well-lit reservoirs, including cold ones, in which phages related to RB49 may occur. This indicates the possibility of more intensive horizontal gene transfer in aquatic ecotopes than previously assumed.</div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 3","pages":"459 - 469"},"PeriodicalIF":4.033,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142859600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of a Combination of Local Moderate Heating and Illumination on the Indicators of Water Metabolism of Intact Parts of Wheat Measured by Thermal Imaging 局部适度加热与光照组合对热成像测定小麦完整部位水分代谢指标的影响

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S0006350924700593

A. Yu. Popova, Yu. A. Zolin, V. S. Sukhov, E. M. Sukhova, L. M. Yudina

{"title":"The Effect of a Combination of Local Moderate Heating and Illumination on the Indicators of Water Metabolism of Intact Parts of Wheat Measured by Thermal Imaging","authors":"A. Yu. Popova, Yu. A. Zolin, V. S. Sukhov, E. M. Sukhova, L. M. Yudina","doi":"10.1134/S0006350924700593","DOIUrl":"10.1134/S0006350924700593","url":null,"abstract":"<div>Natural stress factors can lead to crop loss; when they act locally, stress signals spread, modifying the physiological state and increasing plant resistance. The purpose of this work was to study the effect of a combination of local factors on water metabolism under watering and drought. Wheat was grown in a greenhouse cabinet; drought was created by stopping watering. To assess the water metabolism, a modified index of stomatal conductivity measured by a thermal imager, the water conductivity of the leaf, and the relative water content in the leaves were used. It was found that the conductivity index decreased during drought and had a strong correlation with the hydration status of the plant (R > 0.7, p < 0.05). Under watering, the combined effect led to a decrease in the conductivity index compared to the condition without stimulation; the response decreased with increasing the distance from the stimulation zone. Soil drought reduced the amplitude of changes in the conductivity index. The local action of heating alone and illumination alone did not cause conductivity index changes. The results showed that the local effect of the combination of heating and illumination caused stress signals that reduced the water metabolism of wheat. Potentially, such signals might be of an electrical nature; however, the absence of changes in the conductivity index under drought and the effect of heating alone did not confirm this hypothesis.</div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 3","pages":"522 - 532"},"PeriodicalIF":4.033,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142859645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Genistein on the Structure of Nucleosomes and the Formation of Complexes with PARP1 染料木素对核小体结构及与PARP1复合物形成的影响

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S0006350924700441

T. V. Andreeva, A. V. Efremenko, A. V. Feofanov, A. V. Lyubitelev, A. N. Korovina, V. M. Studitsky, N. V. Maluchenko

引用次数: 0

Azobenzene-Based Voltage-Gated Sodium Channel Blockers with Light-Controlled Local Anesthetic and Antiarrhythmic Activity 偶氮苯基电压门控钠通道阻滞剂具有光控局部麻醉和抗心律失常活性

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S0006350924700568

A. N. Noev, S. G. Kovalenko, E. D. Gataulina, E. A. Turchaninova, V. D. Dzhabrailov, A. A. Aitova, D. A. Likhobabina, J. A. Sutemieva, S. R. Frolova, L. E. Ruppel, D. A. Minakov, N. V. Suvorov, P. V. Ostroverkhov, Yu. L. Vasil’ev, M. V. Nikolaev, V. A. Tsvelaya, K. I. Agladze, M. A. Grin

{"title":"Azobenzene-Based Voltage-Gated Sodium Channel Blockers with Light-Controlled Local Anesthetic and Antiarrhythmic Activity","authors":"A. N. Noev, S. G. Kovalenko, E. D. Gataulina, E. A. Turchaninova, V. D. Dzhabrailov, A. A. Aitova, D. A. Likhobabina, J. A. Sutemieva, S. R. Frolova, L. E. Ruppel, D. A. Minakov, N. V. Suvorov, P. V. Ostroverkhov, Yu. L. Vasil’ev, M. V. Nikolaev, V. A. Tsvelaya, K. I. Agladze, M. A. Grin","doi":"10.1134/S0006350924700568","DOIUrl":"10.1134/S0006350924700568","url":null,"abstract":"The work is devoted to the synthesis of new light-controlled voltage-gated sodium (Nav) channel blockers based on azobenzene and the study of their local anesthetic and antiarrhythmic properties. The effect of ethercaine and two of its new derivatives on native Nav channels was studied in vitro using the patch-clamp method on isolated excitable cells (neurons and cardiomyocytes) of a rat, and local anesthetic activity of the compounds was evaluated on rabbit’s eye cornea. Studies of the effect of the obtained compounds on the conductivity of excitation waves in cardiomyocyte cultures using the optical mapping method were performed. The effective light-dependent biological activity of ethercaine and its derivatives allows us to consider the resulting compounds as potential tools for light-controlled local anesthesia, as well as for non-invasive ablation of arrhythmia in the heart in cardiology.","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 3","pages":"485 - 503"},"PeriodicalIF":4.033,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142859535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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The Involvement of Carbonic Anhydrases in Chloroplasts of C3 Higher Plants in Adaptation Changes of Photosynthetic Reactions C3高等植物叶绿体碳酸酐酶参与光合反应的适应性变化

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S0006350924700477

B. N. Ivanov, N. N. Rudenko

引用次数: 0

Prevention of Mitochondrial Dysfunction with 2-Ethyl-6-Methyl-3-Hydroxypyridine Carnitinate 用 2-乙基-6-甲基-3-羟基吡啶 Carnitinate 预防线粒体功能障碍

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S000635092470026X

I. V. Zhigacheva, I. F. Rusina, N. I. Krikunova, Yu. V. Kuznetsov, M. M. Rasulov, M. A. Yakovleva, A. N. Goloshchapov

{"title":"Prevention of Mitochondrial Dysfunction with 2-Ethyl-6-Methyl-3-Hydroxypyridine Carnitinate","authors":"I. V. Zhigacheva, I. F. Rusina, N. I. Krikunova, Yu. V. Kuznetsov, M. M. Rasulov, M. A. Yakovleva, A. N. Goloshchapov","doi":"10.1134/S000635092470026X","DOIUrl":"10.1134/S000635092470026X","url":null,"abstract":"<div>The biological activity of 2-ethyl-6-methyl-3-hydroxypyridine carnitinate was studied. The drug showed high antiradical and antioxidant activity; this could indicate anti-stress properties, which were studied on a model of acute hypobaric hypoxia. Acute hypobaric hypoxia activated lipid peroxidation by 2.3 times, which resulted in changes in the content of C18 and C20 fatty acids in mitochondrial membranes; the index of double bonds of C18 fatty acids decreased by 18.2%, the content of 20:3ω3 by 13%, 20:2ω6 by 80% and 20:1ω9 by 33%. These changes were accompanied by changes in the bioenergetic characteristics of mitochondria. The maximum oxidation rates of NAD-dependent substrates decreased by 28–35%. The administration of 10–6 mol/kg of 2-ethyl-6-methyl-3-hydroxypyridine carnitinate to animals for 5 days inhibited lipid peroxidation, prevented changes in the composition of fatty acids of mitochondrial membranes, and, consequently, changes in the bioenergetic characteristics of mitochondria; this probably determined the anti-stress properties of the drug, as indicated by an increase in life expectancy by 3.5–4.0 times and an increase in the survival rate of mice by 12–40% under conditions of various types of hypoxia. In addition, the drug stimulated the germination and growth of wheat seedlings.</div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"252 - 260"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Study of the Effect of Sodium-μ2-Dithiosulphate-Tetranitrosyl Diferrate Tetrahydrate on the Structure of Mitochondrial Membranes Isolated from Epicotyls of Pea Seedlings 从豌豆幼苗外胚叶中分离的线粒体膜结构对四水合钠-μ2-二硫代硫酸盐-四亚硝基二重酸盐影响的研究

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700271

N. Yu. Gerasimov, O. V. Nevrova, I. V. Zhigacheva, N. I. Krikunova, A. K. Vorobyova, I. P. Generozova, A. N. Goloshchapov

{"title":"A Study of the Effect of Sodium-μ2-Dithiosulphate-Tetranitrosyl Diferrate Tetrahydrate on the Structure of Mitochondrial Membranes Isolated from Epicotyls of Pea Seedlings","authors":"N. Yu. Gerasimov, O. V. Nevrova, I. V. Zhigacheva, N. I. Krikunova, A. K. Vorobyova, I. P. Generozova, A. N. Goloshchapov","doi":"10.1134/S0006350924700271","DOIUrl":"10.1134/S0006350924700271","url":null,"abstract":"<div>Nitric oxide is a signaling molecule of plants under stressful conditions; this molecule can also have a toxic effect. A study of the dose-dependent effect of the nitric oxide donor sodium-μ2-dithiosulphate-tetranitrosyl diferrate tetrahydrate on the structural state of mitochondrial membranes of epicotyles of pea seedlings has been carried out. Upon treating mitochondria with this drug at a concentration of 10–8 M, thermally induced structural transitions were observed at temperatures from 15°C to 21°C and from 30°C to 36°C in lipid regions, and at temperatures from 12°C to 15°C and from 27°C to 33°C in the protein regions of the membranes. The drug showed antioxidant properties in the lipid regions of the membranes, leading to the accumulation of long unsaturated fatty acids. Treatment of mitochondria with sodium-μ2-dithiosulphate-tetranitrosyl diferrate tetrahydrate at a dose of 10–4 M caused a significant increase in the level of lipid peroxidation and the fluidity of the lipid phase of membranes.</div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"245 - 251"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0