CRISPR/Cas System Photocontrolled at the Guide RNA Level

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology
L. V. Sakovina, E. S. Gorlenko, D. S. Novopashina
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引用次数: 0

Abstract

Improving the efficiency and precision of gene editing systems is of utmost importance for modern molecular biology and genetic engineering. Of particular interest is the design of controlled CRISPR/Cas9 systems, the activity of which could be regulated using various physicochemical stimuli, such as light irradiation, pH change, temperature, change of molecule concentration, and others. A promising direction in this area is the development of approaches to control activity at the level of guide RNA through photosensitive modifications to the structure and sequence of guide RNA, and additional oligonucleotides. This review focuses on the analysis of publications on design of photosensitive guide RNAs and their applications in gene editing systems using CRISPR.

Abstract Image

CRISPR/Cas 系统在引导 RNA 水平上进行光控
提高基因编辑系统的效率和精度对现代分子生物学和基因工程至关重要。特别令人感兴趣的是可控CRISPR/Cas9系统的设计,其活性可以通过各种物理化学刺激来调节,如光照射、pH变化、温度、分子浓度变化等。该领域的一个有希望的方向是通过光敏修饰引导RNA的结构和序列以及额外的寡核苷酸来控制引导RNA水平上的活性。本文综述了光敏引导rna的设计及其在CRISPR基因编辑系统中的应用。
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来源期刊
Biophysics
Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍: Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.
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