IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S000635092470026X

I. V. Zhigacheva, I. F. Rusina, N. I. Krikunova, Yu. V. Kuznetsov, M. M. Rasulov, M. A. Yakovleva, A. N. Goloshchapov

{"title":"Prevention of Mitochondrial Dysfunction with 2-Ethyl-6-Methyl-3-Hydroxypyridine Carnitinate","authors":"I. V. Zhigacheva, I. F. Rusina, N. I. Krikunova, Yu. V. Kuznetsov, M. M. Rasulov, M. A. Yakovleva, A. N. Goloshchapov","doi":"10.1134/S000635092470026X","DOIUrl":"10.1134/S000635092470026X","url":null,"abstract":"<div><p>The biological activity of 2-ethyl-6-methyl-3-hydroxypyridine carnitinate was studied. The drug showed high antiradical and antioxidant activity; this could indicate anti-stress properties, which were studied on a model of acute hypobaric hypoxia. Acute hypobaric hypoxia activated lipid peroxidation by 2.3 times, which resulted in changes in the content of C18 and C20 fatty acids in mitochondrial membranes; the index of double bonds of C18 fatty acids decreased by 18.2%, the content of 20:3ω3 by 13%, 20:2ω6 by 80% and 20:1ω9 by 33%. These changes were accompanied by changes in the bioenergetic characteristics of mitochondria. The maximum oxidation rates of NAD-dependent substrates decreased by 28–35%. The administration of 10<sup>–6</sup> mol/kg of 2-ethyl-6-methyl-3-hydroxypyridine carnitinate to animals for 5 days inhibited lipid peroxidation, prevented changes in the composition of fatty acids of mitochondrial membranes, and, consequently, changes in the bioenergetic characteristics of mitochondria; this probably determined the anti-stress properties of the drug, as indicated by an increase in life expectancy by 3.5–4.0 times and an increase in the survival rate of mice by 12–40% under conditions of various types of hypoxia. In addition, the drug stimulated the germination and growth of wheat seedlings.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"252 - 260"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Study of the Effect of Sodium-μ2-Dithiosulphate-Tetranitrosyl Diferrate Tetrahydrate on the Structure of Mitochondrial Membranes Isolated from Epicotyls of Pea Seedlings 从豌豆幼苗外胚叶中分离的线粒体膜结构对四水合钠-μ2-二硫代硫酸盐-四亚硝基二重酸盐影响的研究

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700271

N. Yu. Gerasimov, O. V. Nevrova, I. V. Zhigacheva, N. I. Krikunova, A. K. Vorobyova, I. P. Generozova, A. N. Goloshchapov

{"title":"A Study of the Effect of Sodium-μ2-Dithiosulphate-Tetranitrosyl Diferrate Tetrahydrate on the Structure of Mitochondrial Membranes Isolated from Epicotyls of Pea Seedlings","authors":"N. Yu. Gerasimov, O. V. Nevrova, I. V. Zhigacheva, N. I. Krikunova, A. K. Vorobyova, I. P. Generozova, A. N. Goloshchapov","doi":"10.1134/S0006350924700271","DOIUrl":"10.1134/S0006350924700271","url":null,"abstract":"<div><p>Nitric oxide is a signaling molecule of plants under stressful conditions; this molecule can also have a toxic effect. A study of the dose-dependent effect of the nitric oxide donor sodium-μ2-dithiosulphate-tetranitrosyl diferrate tetrahydrate on the structural state of mitochondrial membranes of epicotyles of pea seedlings has been carried out. Upon treating mitochondria with this drug at a concentration of 10<sup>–8</sup> M, thermally induced structural transitions were observed at temperatures from 15°C to 21°C and from 30°C to 36°C in lipid regions, and at temperatures from 12°C to 15°C and from 27°C to 33°C in the protein regions of the membranes. The drug showed antioxidant properties in the lipid regions of the membranes, leading to the accumulation of long unsaturated fatty acids. Treatment of mitochondria with sodium-μ2-dithiosulphate-tetranitrosyl diferrate tetrahydrate at a dose of 10<sup>–4</sup> M caused a significant increase in the level of lipid peroxidation and the fluidity of the lipid phase of membranes.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"245 - 251"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacological Blockade of High Threshold L-Type Calcium Channels in Cardiomyocytes Restores Respiratory Chain Activity Impaired by Vibration 药理阻断心肌细胞中的高阈值 L 型钙通道可恢复受振动影响的呼吸链活动

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700301

V. V. Vorobieva, O. S. Levchenkova, P. D. Shabanov

{"title":"Pharmacological Blockade of High Threshold L-Type Calcium Channels in Cardiomyocytes Restores Respiratory Chain Activity Impaired by Vibration","authors":"V. V. Vorobieva, O. S. Levchenkova, P. D. Shabanov","doi":"10.1134/S0006350924700301","DOIUrl":"10.1134/S0006350924700301","url":null,"abstract":"<div><p>The transfer of calcium ions through the cell membrane of a cardiomyocyte is associated with overcoming a high energy barrier and can be impaired by vibration. Since drugs of the calcium channel blocker group are able to reduce damage to mitochondria and cell membranes, the effect of the calcium channel blocker nifedipine (adalat) at a dose of 7.5 mg/kg on the energy metabolism of cardiomyocytes in a vibration-mediated (56 sessions of vibration with a frequency of 44 Hz and an amplitude of 0.5 mm) model of cellular hypoxia was evaluated. The functional states of mitochondria in the composition of 30% homogenate of the myocardial tissue of the left ventricle of the rabbit heart were studied using the polarographic method with a closed galvanic oxygen sensor in a thermostatically controlled 1 mL cuvette with a protein content of 2.4 ± 0.5 mg, measured by a modified microbiuretic assay. In animals subjected to vibration in the presence of calcium channel blockers, the rate of endogenous respiration (<i>V</i><sub>e</sub>) remained at the level of intact animals and was 16.4 ± 4.1 [ng-atom O] min<sup>–1</sup> mg<sup>–1</sup> protein; sensitivity to amytal increased by 39% and sensitivity to malonate decreased by 40% compared with the indicators in animals subjected to vibration without pharmacological protection. The indicators of the ratio of the activity of mitochondrial enzyme complexes I and II and the processes of regulation in different metabolic states of mitochondria confirmed the restoration of the activity of mitochondrial enzyme complex I suppressed by vibration and the regulatory restriction of mitochondrial enzyme complex II. The revealed energy-protective effect of calcium channel blockers increased the vibration resistance of the tissue, preventing the development of necrotic changes in it.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"261 - 266"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Viral Lysis on Light Absorption by the Black Sea Microalgae Tetraselmis viridis 病毒溶解对黑海微藻 Tetraselmis viridis 光吸收的影响

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700313

S. A. Sholar, O. A. Stepanova

引用次数: 0

The Relationship between the Temperature in the Deep Layers of the Somatosensory Cortex and Blood Flow Velocity in the Brain of Anesthetized Mice 躯体感觉皮层深层温度与麻醉小鼠大脑血流速度之间的关系

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700374

A. M. Romshin, A. A. Osypov, V. K. Krohaleva, S. G. Zhuravlev, O. N. Egorova, I. I. Vlasov, I. Yu. Popova

{"title":"The Relationship between the Temperature in the Deep Layers of the Somatosensory Cortex and Blood Flow Velocity in the Brain of Anesthetized Mice","authors":"A. M. Romshin, A. A. Osypov, V. K. Krohaleva, S. G. Zhuravlev, O. N. Egorova, I. I. Vlasov, I. Yu. Popova","doi":"10.1134/S0006350924700374","DOIUrl":"10.1134/S0006350924700374","url":null,"abstract":"<div><p>Despite the obvious importance of temperature for brain functioning, it remains one of the least studied parameters of the brain. The temperature is determined by the balance between the release of heat during metabolism and its removal by the bloodstream, which is regulated by neurovascular coupling. Using the method of laser speckle contrast imaging and micro-diamond thermometry, we performed a long-term simultaneous recording of blood flow velocity and temperature in the brain of anesthetized animals <i>in vivo</i> for the first time. To study the coupling between temperature and blood flow two approaches were used: enhancing the general blood flow by intraperitoneal injection of adrenaline and enhancing neuronal activity by applying KCl solution to the brain surface. The data we obtained indicated that the temperature of the nerve tissue had a more complex dynamics compared to blood flow, which was apparently associated with direct or indirect activation of both individual neurons and neural ensembles. Studies of temperature dynamics can make a significant contribution to understanding the nature of neurovascular coupling.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"306 - 313"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the Role of Priming in the Development of Modern Rehabilitation Technologies 论引导在现代康复技术发展中的作用

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700428

A. I. Fedotchev

引用次数: 0

Biophysical Methods for Testing Nanomaterials for Biomedical and Ecotoxicological Purposes Using Daphnia magna as a Model Organism 以大型蚤为模式生物测试用于生物医学和生态毒理学目的的纳米材料的生物物理方法

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700386

N. B. Savina, D. V. Uskalova, D. T. Petrosova, E. I. Sarapultseva

引用次数: 0

Ambiol Modulates the Functional Characteristics of Pea Seedlings Mitochondria 安倍醇调节豌豆幼苗线粒体的功能特征

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700295

I. V. Zhigacheva, N. I. Krikunova, Yu. V. Kuznetsov, A. N. Goloshchapov

{"title":"Ambiol Modulates the Functional Characteristics of Pea Seedlings Mitochondria","authors":"I. V. Zhigacheva, N. I. Krikunova, Yu. V. Kuznetsov, A. N. Goloshchapov","doi":"10.1134/S0006350924700295","DOIUrl":"10.1134/S0006350924700295","url":null,"abstract":"<div><p>The effect of the plant growth regulator ambiol on the functional state of mitochondria of etiolated pea seedlings was studied. The drug prevented the activation of lipid peroxidation in mitochondrial membranes in the concentration range of 10<sup>–5</sup>–10<sup>–6</sup> and 10<sup>–9</sup> M. The introduction of ambiol into the incubation medium of these organelles resulted in an increase in the oxidation rates of NAD-dependent substrates. The most effective concentrations were 10<sup>–5</sup> and 10<sup>–6</sup> M. Changes in the bioenergetic characteristics of mitochondria were accompanied by changes in the fatty acid composition of mitochondrial membranes. The most significant changes occurred in the content of 18:2ω6 and 18:3ω3 fatty acids. It was hypothesized that an increase in the content of these fatty acids in the membranes of mitochondria indicated an increase in the content of cardiolipin, which ensured the effective functioning of the mitochondrial respiratory chain and, consequently, the maintenance of energy metabolism at a high level. The treatment of pea seeds with ambiol not only prevented a decrease in the growth rate of pea seedlings under conditions of water stress, but also stimulated their growth.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"231 - 236"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Combined Effects of Electrochemical and Ultrasonic Treatment for Water Purification from Pathogenic Contaminants Based on the Example of Escherichia coli 以大肠埃希氏菌为例，探讨电化学和超声波处理对净化水中致病污染物的联合作用

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700325

S. B. Bibikov, A. I. Sergeev, I. I. Barashkova, M. V. Motyakin

引用次数: 0

The Relationship between the Composition of Natural Water and State Lipid Peroxidation in Biological Objects 天然水成分与生物体内脂质过氧化状态之间的关系

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700350

L. N. Shishkina, A. S. Dubovik, V. O. Shvydkiy, M. V. Kozlov, E. V. Shtamm, A. G. Georgiadi

{"title":"The Relationship between the Composition of Natural Water and State Lipid Peroxidation in Biological Objects","authors":"L. N. Shishkina, A. S. Dubovik, V. O. Shvydkiy, M. V. Kozlov, E. V. Shtamm, A. G. Georgiadi","doi":"10.1134/S0006350924700350","DOIUrl":"10.1134/S0006350924700350","url":null,"abstract":"<div><p>The relationship between the compositions of five natural water samples and the parameters of the lipid peroxidation regulation in a model system based on natural phospholipids, as well as their participation in the toxicity of natural water, have been studied. It has been shown that the presence of N- and P-containing compounds in natural water samples led to inhibition of lecithin autooxidation and luminescence intensity of luminous bacteria; it had a significant effect on spontaneous aggregation of lecithin and an increase in the content of hydrophobic compounds increased the negative value of the ζ potential of its particles. The high sensitivity of the parameters of the lipid peroxidation regulation system to the composition of natural water, even at low concentrations of its components, allows them to be considered as promising indicators for assessing the effect of natural water on biological objects. Mathematical processing of ultraviolet spectra of natural water samples using the Gauss method can be used as an express test for the analysis of its hydrochemical composition. The effect of natural water components on the state of membranes and intracellular processes of biological objects has been confirmed by biotesting methods.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"292 - 298"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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