Biophysics最新文献

Nitrosonium Cation as an Antitumor Component of Dinitrosyl Iron Complexes with Thiol-containing Ligands

IF 4.033

Biophysics Pub Date : 2025-03-17 DOI: 10.1134/S0006350924701288

A. F Vanin, L. A. Ostrovskaya, D. B. Korman, V. A. Rykova, N. V. Bluhterova, M. M. Fomina, V. D. Mikoyan, N. A. Tkachev

{"title":"Nitrosonium Cation as an Antitumor Component of Dinitrosyl Iron Complexes with Thiol-containing Ligands","authors":"A. F Vanin, L. A. Ostrovskaya, D. B. Korman, V. A. Rykova, N. V. Bluhterova, M. M. Fomina, V. D. Mikoyan, N. A. Tkachev","doi":"10.1134/S0006350924701288","DOIUrl":"10.1134/S0006350924701288","url":null,"abstract":"<div><p>The combined use of two compounds, the binuclear form of dinitrozyl iron complexes with glutathione (100 µM/kg, subcutaneously) and sodium diethyldithiocarbamate (500 µM/kg, intraperitoneally), administered 8 times, caused complete inhibition of the development of a solid tumor in mice for 2 weeks after transplantation (Lewis lung carcinoma). However, when the antitumor effect was assessed on the 20th day after the end of drug administration, the maximum activity, inhibition of tumor growth by 60%, was observed when the drugs were administered in the sequence “diethyldithiocarbamate, and then 1 h later binuclear dinitrozyl iron complexes”; whereas when the drugs were used in the reverse sequence, inhibition of tumor growth did not exceed 30%. Based on the analysis of EPR measurements of tumor tissues (on the 15th day of tumor development), it was concluded that the inhibition of tumor growth was caused by nitrosonium cations released from binuclear dinitrosyl iron-glutathione complexes during the breakdown of these complexes under the action of sodium diethyldithiocarbamate.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 6","pages":"1195 - 1201"},"PeriodicalIF":4.033,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polysaccharide from Solanum tuberosum L. as a Potential Antiulcer Drug 作为潜在抗溃疡药物的茄属多糖

IF 4.033

Biophysics Pub Date : 2025-03-17 DOI: 10.1134/S0006350924701276

L. V. Generalova, K. A. Kritskaya, D. P. Laryushkin, E. A. Generalov

引用次数: 0

The Protective Effect of Low-Intensity Extremely-High-Frequency Electromagnetic Radiation on an Experimental Model of Intestinal Dysbiosis in Laboratory Rats 低强度极高频电磁辐射对实验鼠肠道菌群失调实验模型的保护作用

IF 4.033

Biophysics Pub Date : 2025-03-17 DOI: 10.1134/S0006350924701252

A. B. Gapeyev, T. P. Kulagina, E. S. Zhukova, A. V. Aripovsky, M. A. Pozdnyakova

{"title":"The Protective Effect of Low-Intensity Extremely-High-Frequency Electromagnetic Radiation on an Experimental Model of Intestinal Dysbiosis in Laboratory Rats","authors":"A. B. Gapeyev, T. P. Kulagina, E. S. Zhukova, A. V. Aripovsky, M. A. Pozdnyakova","doi":"10.1134/S0006350924701252","DOIUrl":"10.1134/S0006350924701252","url":null,"abstract":"<div><p>Using an experimental model of dysbiosis caused by the antibiotic gentamicin sulfate, the effect of low-intensity electromagnetic radiation of extremely high frequencies (42.2 GHz, 100 µW/cm<sup>2</sup>, pulse modulation by a meander with a frequency of 1 Hz, exposure for 30 min per day for 10 consecutive days, starting from the 4th day after induction of dysbiosis) on the fatty acid composition of the thymus, blood plasma, activity of antioxidant enzymes, the content of malondialdehyde and lactate in the blood of laboratory rats was studied. A significant decrease in the content of myristic, palmitic, stearic, palmitoleic, oleic and linoleic fatty acids in the thymus of animals was shown. The activity of antioxidant enzymes, the content of fatty acids and malonic dialdehyde in the blood did not change. The content of lactate in the blood plasma decreased. Irradiation of animals with dysbiosis normalized the fatty acid composition of the thymus and the content of lactate in plasma. The high efficiency of low-intensity extremely-high-frequency electromagnetic radiation with certain parameters in dysbiosis opens up fundamentally new possibilities for using radiation in this range for the prevention and treatment of a number of pathological conditions associated with dysbiosis.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 6","pages":"1161 - 1168"},"PeriodicalIF":4.033,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycerol Permeation Through the Human Sperm Membrane During Cryopreservation

IF 4.033

Biophysics Pub Date : 2025-03-17 DOI: 10.1134/S0006350924701094

A. A. Ivanova, I. I. Leonov, E. S. Gnilozub-Volobueva, M. A. Ovchinnikov, S. A. Yakovenko, E. Yu. Simonenko

{"title":"Glycerol Permeation Through the Human Sperm Membrane During Cryopreservation","authors":"A. A. Ivanova, I. I. Leonov, E. S. Gnilozub-Volobueva, M. A. Ovchinnikov, S. A. Yakovenko, E. Yu. Simonenko","doi":"10.1134/S0006350924701094","DOIUrl":"10.1134/S0006350924701094","url":null,"abstract":"<div><p>Cryopreservation of biological material is an important issue in many fields of biology and medicine, especially for assisted reproductive technologies. Cryoprotective media, including cell-permeating and non-permeating components, are used for effective cryopreservation of cells. Glycerol at various concentrations is often used as a permeating component. However, elucidating the mechanisms of the cryoprotective effect of these compounds, as well as increasing the proportion of surviving cells after cryopreservation, remain urgent tasks of cryobiology. In this study, the mechanism of glycerol transfer through sperm membranes with the participation of aquaporins was investigated by mathematical modeling. The presented model describes the dynamics of changes in sperm volume depending on the concentration of glycerol and sucrose in a cryoprotective medium. As a result of the analysis, the characteristic time for establishing equilibrium between the cryoprotective medium under study and the cells was calculated, which was <i>t</i> = 80 s in the presence of 12 vol. % of glycerol. The sucrose concentration (0.16 M) was determined at which the cell returns to its original volume after incubation in the cryoprotective medium. It has been shown that the human sperm can contain 6750 aquaporins of the 7th type with a permeability <i>P</i><sub>AQP7</sub>= 0.00366 µm<sup>3</sup>/s. The experiments performed showed an increase in the sperm motility index after adjusting the sucrose concentration in the solution in accordance with the simulation results.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 6","pages":"1010 - 1018"},"PeriodicalIF":4.033,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiviral Effect Against SARS-CoV-2 of Dinitrosyl Iron Complex Solutions when Inhaled to Syrian Hamsters in a “Nose-Only” Chamber 叙利亚仓鼠在 "仅鼻腔 "中吸入二亚硝基铁复合物溶液对 SARS-CoV-2 的抗病毒效果

IF 4.033

Biophysics Pub Date : 2025-03-17 DOI: 10.1134/S0006350924701227

A. V. Shipovalov, A. F. Vanin, N. A. Tkachev, O. V. Pyankov, N. B. Asanbaeva, S. V. An’kov, E. G. Bagryanskaya, A. M. Baklanov, S. V. Valiulin, M. E. Stekleneva

{"title":"Antiviral Effect Against SARS-CoV-2 of Dinitrosyl Iron Complex Solutions when Inhaled to Syrian Hamsters in a “Nose-Only” Chamber","authors":"A. V. Shipovalov, A. F. Vanin, N. A. Tkachev, O. V. Pyankov, N. B. Asanbaeva, S. V. An’kov, E. G. Bagryanskaya, A. M. Baklanov, S. V. Valiulin, M. E. Stekleneva","doi":"10.1134/S0006350924701227","DOIUrl":"10.1134/S0006350924701227","url":null,"abstract":"<div><p>There was no noticeable change in the antiviral effect of inhalation of solutions of binuclear dinitrozyl iron complexes with glutathione (B-DNIC-GSH) and sodium diethyldithiocarbamate (DETC) to Syrian hamsters infected with the SARS-CoV-2 virus when they were successively administered to animals in a “nose-only” chamber or a “whole-body” chamber. Inhalation in the “whole-body” chamber, which led to wetting of the animals’ bodies, led only to a decrease in the level of mononuclear dinitrosyl iron complexes with thiol groups of proteins recorded by the EPR method in the lungs, without changing the total content of the binuclear and mononuclear forms of dinitrosyl iron complexes. It is assumed that the antiviral effect of B-DNIC-GSH + DETC against the SARS-CoV-2 virus was due to nitrosonium (NO<sup>+</sup>) cations released from B-DNIC-GSH as a result of its destruction by DETC molecules. A similar antiviral effect on the Syrian hamster models was exerted by a less stable than B-DNIC-GSH complex with mercaptosuccinate, without additional aerosol treatment of animals with a solution of DETC.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 6","pages":"1137 - 1146"},"PeriodicalIF":4.033,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of the Functional State of Cardiac Mitochondria in Inbred Mice with Type 2 Diabetes Mellitus 近交系 2 型糖尿病小鼠心脏线粒体功能状态研究

IF 4.033

Biophysics Pub Date : 2025-03-17 DOI: 10.1134/S0006350924701112

Y. L. Baburina, I. V. Odinokova, R. R. Krestinin, A. I. Zvyagina, L. D. Sotnikova, O. V. Krestinina

{"title":"Study of the Functional State of Cardiac Mitochondria in Inbred Mice with Type 2 Diabetes Mellitus","authors":"Y. L. Baburina, I. V. Odinokova, R. R. Krestinin, A. I. Zvyagina, L. D. Sotnikova, O. V. Krestinina","doi":"10.1134/S0006350924701112","DOIUrl":"10.1134/S0006350924701112","url":null,"abstract":"<div><p>The origin of diabetes mellitus, one of the most common metabolic diseases in the developed world, is associated with impaired insulin secretion or cell resistance to the effect of this hormone (type I and type II diabetes, respectively). In both cases, a common pathological change is an increase in blood glucose level, hyperglycemia, which can eventually lead to serious damage to organs and tissues of the body. Mitochondria are believed to be one of the main targets of diabetes at the intracellular level. In this study, we investigated the functional state of the mitochondria of the heart of inbred mice of the C57BL/Ks–db+/+m line carrying the recessive <i>diabetes-db</i> gene (diabetic mice). Histological analysis of the left ventricle of the heart of diabetic and control mice was performed. In the heart tissue samples of diabetic mice, an increase in the intensity of eosin staining was observed, which implies damage to the structure of cytoplasmic proteins. In addition, it was shown that respiratory control and Ca<sup>2+</sup> capacity in the mitochondria of diabetic mice decreased compared to controls. Changes in mitochondrial dynamics and mitophagy under these conditions were investigated.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 6","pages":"1028 - 1037"},"PeriodicalIF":4.033,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Administration of Phospholipids with Polyunsaturated Fatty Acids in the Tail Groups Makes it Possible to Prevent a Decrease of Sperm Motility in the Fruit Fly Drosophila melanogaster in the Early Period of Readaptation after Space Flight 在尾部施用含有多不饱和脂肪酸的磷脂可防止果蝇在太空飞行后重新适应的早期精子活力下降

IF 4.033

Biophysics Pub Date : 2025-03-17 DOI: 10.1134/S0006350924701124

I. V. Ogneva, K. K. Gogichaeva, Yu. S. Zhdankina, O. V. Kotov

{"title":"Administration of Phospholipids with Polyunsaturated Fatty Acids in the Tail Groups Makes it Possible to Prevent a Decrease of Sperm Motility in the Fruit Fly Drosophila melanogaster in the Early Period of Readaptation after Space Flight","authors":"I. V. Ogneva, K. K. Gogichaeva, Yu. S. Zhdankina, O. V. Kotov","doi":"10.1134/S0006350924701124","DOIUrl":"10.1134/S0006350924701124","url":null,"abstract":"<div><p>The aim of this study was an attempt to prevent the decrease in sperm motility of the fruit fly <i>Drosophila melanogaster</i> that occurs in the early period of readaptation after space flight by adding phospholipids with polyunsaturated fatty acids in the tail groups (essential phospholipids) to the nutrient medium. The study was carried out as part of space flights on board the Russian segment of the ISS during the periods of September 15, 2023–September 27, 2023 (ISS-69) and March 23, 2024–April 6, 2024 (ISS-70). The content of cytoskeletal proteins in the testes was determined by Western blotting and sperm motility by video recording and subsequent analysis. The results of the study show that modification of the nutrient medium by adding essential phospholipids leads to a decrease in cholesterol content and an increase in actin content, which prevents a decrease in tubulin content and a decrease in the speed of movement of fruit fly sperm in the early period of readaptation (up to 16 hours) after space flight. The data we obtained, on the one hand, demonstrate a possible mechanism for triggering mechanotransduction, and on the other, allow us to propose essential phospholipids as one of the means of protection against negative changes associated with changes in the gravity field acting on the cell.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 6","pages":"1038 - 1044"},"PeriodicalIF":4.033,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Cytoprotective and Antioxidant Effects of Resveratrol on Human Lymphocytes Modified with Hydrogen Peroxide and UV Light

IF 4.033

Biophysics Pub Date : 2025-03-17 DOI: 10.1134/S0006350924701100

M. A. Nakvasina, V. G. Artyukhov, E. N. Chursanova, O. V. Myachina, E. I. Korpusova, A. Yu. Deenkova, V. A. Shestykh

引用次数: 0

The Cytotoxicity of Gold and Silver Polyacrylates for Tumor Cells

IF 4.033

Biophysics Pub Date : 2025-03-17 DOI: 10.1134/S0006350924701148

L. A. Ostrovskaya, D. B. Korman, E. I. Nekrasova, N. V. Bluhterova, U. A. Hochenkova, K. A. Abzaeva

引用次数: 0

The Radioprotective Effect of Exogenous Peroxiredoxin 6 in Mice Exposed to Different Doses of Whole-Body Ionizing Radiation 外源性过氧化还原酶 6 对暴露于不同剂量全身电离辐射的小鼠的辐射防护作用

IF 4.033

Biophysics Pub Date : 2025-03-17 DOI: 10.1134/S0006350924701240

E. E. Karmanova, R. G. Goncharov, V. I. Bruskov, V. I. Novoselov, M. G. Sharapov

{"title":"The Radioprotective Effect of Exogenous Peroxiredoxin 6 in Mice Exposed to Different Doses of Whole-Body Ionizing Radiation","authors":"E. E. Karmanova, R. G. Goncharov, V. I. Bruskov, V. I. Novoselov, M. G. Sharapov","doi":"10.1134/S0006350924701240","DOIUrl":"10.1134/S0006350924701240","url":null,"abstract":"<div><p>The search for effective and safe radio-modulating compounds that reduce cancer cell radioresistance and/or the concomitant damage to normal tissues caused by radiation remains a significant challenge in radiation therapy. Peroxiredoxin 6, a member of the thiol-specific peroxidase family, is a promising candidate to solve this problem. However, often in studies, insufficient attention is paid to radiation parameters and there is no information about how different radiation parameters influence the radioprotective effect of exogenous peroxiredoxin 6. This article shows the peculiarities of the radioprotective effects of intravenous peroxiredoxin 6 at a concentration of 20 μg/g body weight and its mutant form Prx6-C47S (without peroxidase activity) administered shortly before X-ray or γ-irradiation with different dose rates and exposure times. Survival rates of the 10 Gy γ-irradiation 0.125 Gy/min and 7 Gy X-ray irradiation 1 Gy/min animal groups (an 8-fold difference in dose rate and exposure time) were correlated both for control and experimental animals.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 6","pages":"1153 - 1160"},"PeriodicalIF":4.033,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0