IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925700940

L. P. Savochkina, T. N. Belyanko, Z. I. Tsokolaeva, R. Sh. Bibilashvili

{"title":"A Method for Obtaining Covalent Conjugates of QD Particles with Enzymes Involved in Thrombosis and Thrombolysis","authors":"L. P. Savochkina, T. N. Belyanko, Z. I. Tsokolaeva, R. Sh. Bibilashvili","doi":"10.1134/S0006350925700940","DOIUrl":"10.1134/S0006350925700940","url":null,"abstract":"<div><p>Covalent complexes of prourokinase, miniplasmin, and thrombin with QD nanoparticles containing aldehyde, amino, and carboxyl groups on the surface were obtained. It was shown that under the selected immobilization conditions, the listed enzymes immobilized on QD nanoparticles fully retained their enzymatic activity and the formed complexes were stable colloids. The coating density of nanoparticles with enzymes reached 50–100 molecules per particle. The immobilization of enzymes on carboxylic nanoparticles was performed using cabodiimide. Unlike the technology with carboxyl groups, the immobilization of enzymes on aldehyde nanoparticles does not require the use of carbodiimide to form a covalent complex and does not require a procedure for washing reagents before adding enzymes to nanoparticles, which improves the reproducibility of the immobilization procedure. It has been shown that particles with immobilized thrombin, when incubated with fibrinogen or blood plasma, form microemboli, which in vitro are completely destroyed by plasmin in the physiological concentration of the latter.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 5","pages":"871 - 885"},"PeriodicalIF":4.033,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accumulation of Nitric Oxide Stabilized Forms in Rat Organs as a Result of Dinitrosyl Iron Complexes with N-Acetyl-L-Cysteine Ligand Administration 二硝基铁配合物与n -乙酰- l-半胱氨酸配体在大鼠器官中积累一氧化氮稳定形式

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925701118

A. A. Timoshin, V. L. Lakomkin, A. A. Abramov, M. V. Ivanova, K. B. Shumaev

引用次数: 0

Peroxiredoxins as Key Markers of Radioresistance and Potential Targets in Cancer Radiotherapy 过氧化物还毒素是肿瘤放疗中放射耐药的关键标志物和潜在靶点

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925701155

E. E. Karmanova, S. B. Parfenyuk, O. V. Glushkova, D. E. Burmistrov, V. I. Novoselov, M. G. Sharapov

引用次数: 0

Interaction of Ascorbic Acid with Spin-Labeled Derivatives of Stearic Acid Embedded in Liposome Membranes 抗坏血酸与脂质体膜中自旋标记硬脂酸衍生物的相互作用

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925701040

V. A. Medvedeva, M. V. Ivanova, E. K. Ruuge, K. B. Shumaev

{"title":"Interaction of Ascorbic Acid with Spin-Labeled Derivatives of Stearic Acid Embedded in Liposome Membranes","authors":"V. A. Medvedeva, M. V. Ivanova, E. K. Ruuge, K. B. Shumaev","doi":"10.1134/S0006350925701040","DOIUrl":"10.1134/S0006350925701040","url":null,"abstract":"<div><p>The interaction of ascorbic acid with nitroxyl radicals from spin labels of 5-doxyl stearic and 16-doxyl stearic acids embedded in phosphatidylcholine liposomal membranes was studied using electron paramagnetic resonance (EPR) spectroscopy. Ascorbic acid was shown to effectively restore the 5-doxyl stearic acid tag in liposomes. After 90 min of incubation with 10 mM ascorbic acid the EPR signal from this label decreased by 70%. After 20 min, the signal decreased by only 50%. At the same time, there was a decrease in the EPR amplitude of 16-doxyl stearic acid by no more than 35% after incubation of the liposomes for 120 min at a concentration of 100 mM ascorbic acid. It is also significant that the signal of 16-doxyl stearic acid remained stable for more than 3 hours. During the reduction of 5-doxyl stearic acid an ascorbate radical was formed but this was not observed for 16-doxyl stearic acid. This is likely due to the limited diffusion of ascorbic acid into the bilayer. In the absence of active transport, ascorbic acid is able to penetrate the membrane and interact with radicals near the polar region, reaching the level of 5-doxyl stearic acid tags. These findings are important for our understanding of the antioxidant role of vitamin C in lipid membranes.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 6","pages":"970 - 976"},"PeriodicalIF":4.033,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of Polyclonal Antibodies Specific to Obscurin of the Long-Tailed Ground Squirrel Urocitellus undulatus: Problems and Prospects 长尾地鼠波尾鼠盲蛋白多克隆抗体的制备：问题与展望

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925701015

T. A. Uryupina, S. S. Popova, G. Z. Mikhailova, L. G. Bobyleva, A. D. Ulanova, A. G. Bobylev, S. N. Udaltsov, N. M. Zakharova, I. M. Vikhlyantsev

引用次数: 0

Brain Biopotentials as a Factor of Automatic Modulation of Therapeutic Sensory Stimulation Parameters 脑生物电位作为治疗性感觉刺激参数自动调节的一个因素

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925700988

A. I. Fedotchev

引用次数: 0

A Study of the Structure and Function of Musculature in Planarians (Platyhelminthes) 拟涡虫（Platyhelminthes）肌肉组织结构与功能的研究

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S000635092570085X

N. D. Kreshchenko

{"title":"A Study of the Structure and Function of Musculature in Planarians (Platyhelminthes)","authors":"N. D. Kreshchenko","doi":"10.1134/S000635092570085X","DOIUrl":"10.1134/S000635092570085X","url":null,"abstract":"<div><p>The functional activity and morphological characteristics of the musculature of planarians have been studied. A physiological study conducted on isolated muscle cells of the planarian <i>Procerodes littoralis</i> (Turbellaria, Platyhelminthes) showed that caffeine in concentrations of 1–10 mM and depolarization caused by a high content of potassium ions (20–75 mM) had a dose-dependent stimulatory effect on the musculature of planarians. Tapsigargin and cyclopiazonic acid, which are calcium reuptake blockers in the sarcoplasmic reticulum depot, reduced the number of muscle responses induced by both caffeine and depolarization. In addition, contractions of single <i>P. littoralis</i> muscle fibers induced by a medium with a high content of potassium ions were inhibited by ryanodine, which in mM concentrations is a blocker of the ryanodine calcium channels of the sarcoplasmic reticulum. The data we obtained suggest that intracellular calcium depots, as an intracellular source of calcium ions, play a role in the mechanism of contractile muscle response in flatworms. Histochemical examination using frozen sections and staining with fluorescently labeled phalloidin demonstrated the conservative organization of the body wall musculature of <i>Schmidtea mediterranea</i> planarians. The musculature of the body wall contains circular, diagonal, and longitudinal muscle fibers. The thinnest circular fibers are densely packed in the outer layer of the musculature. The longitudinal fibers are the thickest and are mostly assembled in pairs. The diagonal fibers extend in two directions at an angle of 110–115 degrees to each other. Dorso-ventral muscle bundles penetrate the planarian body. The intestinal lumen is surrounded by thin longitudinal muscle fibers. These experiments have shown that planarians can be successfully used as a model object for studying the mechanisms of muscle contraction in flatworms.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 5","pages":"796 - 806"},"PeriodicalIF":4.033,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure and Cooperative Interactions between the Guanine Quadruplexes of the Promoter of Gallus gallus βA-Globin Gene 鸡β a -珠蛋白基因启动子鸟嘌呤四联体的结构及协同相互作用

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925700812

E. V. Marilovtseva, D. O. Koshkina, A. V. Feofanov, V. M. Studitsky

{"title":"Structure and Cooperative Interactions between the Guanine Quadruplexes of the Promoter of Gallus gallus βA-Globin Gene","authors":"E. V. Marilovtseva, D. O. Koshkina, A. V. Feofanov, V. M. Studitsky","doi":"10.1134/S0006350925700812","DOIUrl":"10.1134/S0006350925700812","url":null,"abstract":"<div><p>Guanine quadruplexes are secondary nucleic acid structures present in the genomes of all eukaryotes, from yeast to mammals, where they play an important role in maintaining telomere integrity, creating TAD boundaries, and regulating transcription, alternative splicing, and translation. It was found that, contributing to the formation of a nucleosome-free region, guanine quadruplexes formed by two G-rich motifs inside the replication origin localized in the promoter of the <i>Gallus gallus</i> β<sup>A</sup>-globin gene are necessary for the initiation of replication. In our work, circular dichroism spectroscopy was used to study the structures and dynamic properties of guanine quadruplexes formed by the β<sup>A</sup>-globin promoter/origin sequence in vitro. The data obtained show that quadruplexes located on the same DNA fragment are formed cooperatively, influencing the structures of each other and the entire DNA fragment on which they are located. These data suggest that the structures of guanine quadruplexes may be determined by their genomic environment, and also help explain some of the properties of quadruplexes observed in vivo.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 5","pages":"753 - 761"},"PeriodicalIF":4.033,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein Adsorption on Polymeric Nanoparticles: Impact of Polyvinyl Alcohol 聚乙烯醇对蛋白质在高分子纳米颗粒上吸附的影响

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925700848

O. E. Kamaeva, M. B. Sokol, I. A. Gulyaev, M. A. Klimenko, N. G. Yabbarov, M. R. Mollaeva, M. V. Chirkina, S. A. Brezgin, S. L. Kuznetsov, E. D. Nikolskaya

{"title":"Protein Adsorption on Polymeric Nanoparticles: Impact of Polyvinyl Alcohol","authors":"O. E. Kamaeva, M. B. Sokol, I. A. Gulyaev, M. A. Klimenko, N. G. Yabbarov, M. R. Mollaeva, M. V. Chirkina, S. A. Brezgin, S. L. Kuznetsov, E. D. Nikolskaya","doi":"10.1134/S0006350925700848","DOIUrl":"10.1134/S0006350925700848","url":null,"abstract":"<p>The protein corona has a significant impact on the biodistribution, pharmacokinetics and therapeutic functionality of nanosystems, which determines the need for an in-depth study of the factors influencing its formation. The auxiliary substances included in the nanoparticles, in particular, polyvinyl alcohol, can affect their physico-chemical properties, thereby affecting the formation of a protein corona. In this work, nanoparticles based on a copolymer of lactic and glycolic acids loaded with paclitaxel were synthesized by single emulsification method using various concentrations of polyvinyl alcohol. The obtained nanoparticles were characterized by dynamic and electrophoretic light scattering methods. The adsorption models of Langmuir, Freundlich, Langmuir–Freundlich and Brunauer, Emmett and Teller were used to describe the process of protein adsorption on the surface of nanoparticles. The obtained data indicate that the concentration of adsorbed proteins decreases when the residual polyvinyl alcohol concentration on the particle surface is increased. The revealed relationship will allow optimizing the design of nanosystems based on a copolymer of lactic and glycolic acids in order to increase the effectiveness of therapy.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 5","pages":"787 - 795"},"PeriodicalIF":4.033,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and Dimensional Diversity of Gastric Juice Extracellular Vesicles in the Norm and at Gastric Cancer 正常人和胃癌患者胃液细胞外囊泡形态和尺寸的差异

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925701167

A. A. Beliaeva, V. A. Pogosyan, G. O. Skryabin, S. A. Galetsky, D. V. Bagrov, A. V. Moiseenko, T. S. Trifonova, O. T. Imaraliev, I. A. Karasev, E. M. Tchevkina

{"title":"Morphological and Dimensional Diversity of Gastric Juice Extracellular Vesicles in the Norm and at Gastric Cancer","authors":"A. A. Beliaeva, V. A. Pogosyan, G. O. Skryabin, S. A. Galetsky, D. V. Bagrov, A. V. Moiseenko, T. S. Trifonova, O. T. Imaraliev, I. A. Karasev, E. M. Tchevkina","doi":"10.1134/S0006350925701167","DOIUrl":"10.1134/S0006350925701167","url":null,"abstract":"<p>Complex morphological and dimensional profiling of extracellular vesicles of human gastric juice by cryo-electron microscopy has been performed. It has been shown that in gastric cancer, the proportion of classical individual vesicles surrounded by a single layer of the plasma membrane increases statistically significantly (up to 74% versus 40% in healthy controls), while the representation of multilayer forms decreases. A comparative analysis of data from cryo-electron microscopy and analysis of nanoparticle trajectories has been carried out, demonstrating that cryo-electron microscopy reveals a significantly higher proportion of particles in the range of 40–70 nm. Thus, the paper describes for the first time the composition of extracellular vesicles of gastric juice and reveals significant differences in the morphological distribution between the norm and pathology, as well as systematic discrepancies between the methods of cryo-electron microscopy and analysis of nanoparticle trajectories in the direction of greater sensitivity of cryo-electron microscopy to small vesicles.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 6","pages":"1073 - 1081"},"PeriodicalIF":4.033,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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