1H-NMR Spectroscopy of Blood Plasma for Detection of Changes in Metabolism during the Development of M-1 Sarcoma in Rats

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology

Biophysics Pub Date : 2025-02-26 DOI:10.1134/S0006350924700829

A. Y. Egorov, A. S. Bykov, T. I. Ponomareva, M. V. Molchanov, N. M. Pankratova, A. N. Pankratov, A. G. Arakelyan, S. N. Koryakin, M. A. Timchenko

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Abstract

The effectiveness of ¹H-NMR-based metabolomic analysis for the detection of metabolic changes during carcinogenesis was assessed based on a comparison of the quantitative composition of metabolites in the blood plasma of healthy rats and rats that receiving M-1 sarcoma grafts. Plasma was collected from the rats under study on day 12 and day 36 after sarcoma transplantation to identify metabolites associated with tumor development. Analysis of the NMR spectra using multivariate statistical methods showed differences in the composition of metabolites for the groups of animals under study as soon as on day 12 after sarcoma transplantation; on day 36 the differences were significant. Twenty three metabolites were quantified. On day 12, only the lactate and allantoin levels were significantly different between the groups, while on day 36, the levels of nine metabolites were different between the two groups. All of the identified metabolites are involved in cancer metabolism, which makes ¹H-NMR spectroscopy a promising method for cancer diagnosis.

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来源期刊

Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

1.20

自引率

0.00%

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期刊介绍： Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.