阳离子防腐剂对光系统I和II分离光合配合物光谱特性和电子传递的影响

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology

Biophysics Pub Date : 2024-12-20 DOI:10.1134/S0006350924700519

V. Z. Paschenko, E. P. Lukashev, M. D. Mamedov, D. A. Gvozdev, B. N. Korvatovsky, P. P. Knox, M. G. Strakhovskaya

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引用次数: 0

摘要

研究了阳离子抗菌剂（微摩尔浓度）对具有完整水氧化配合物的菠菜中PSII活性核心配合物和蓝细菌PCC6803中PSII活性核心配合物的影响。在所研究的防腐剂（米拉米司汀、氯己定、西替尼定和匹氯代）中，西替尼定的效果最好。综上所述，其对PSII的影响主要在于影响了光收集天线（CP43和CP47）的结构，激发能通过该结构传递到反应中心。结果，这种结构的叶绿素分子不稳定，其光学和功能特性发生了变化。在PSI的蓝藻复合体中也观察到类似的效果。此外，防腐剂还影响了PSI天线复合物中激发态平衡分布的建立速度。辛替尼啶对PS I络合物中的电子转移速率也有显著影响：在辛替尼啶的存在下，光活性色素P700与末端受体FA/FB之间的光分离电荷的重组速度提高了一倍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

The Effects of Cationic Antiseptics on Spectral Characteristics and Electron Transport in Isolated Photosynthetic Complexes of Photosystems I and II

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The Effects of Cationic Antiseptics on Spectral Characteristics and Electron Transport in Isolated Photosynthetic Complexes of Photosystems I and II

The effect of cationic antiseptics (at micromolar concentrations) on PSII active core complexes isolated from spinach plants with an intact water oxidation complex and on PSI core complexes from cyanobacterium Synechocystis sp. PCC6803 were studied. Of the antiseptics studied (miramistin, chlorhexidine, octenidine, and picloxydine), octenidine had the greatest effect. It was concluded that its effect on PSII consisted primarily in affecting the structure of the light-harvesting antenna (CP43 and CP47), through which the excitation energy is delivered to the reaction center. As a result, the chlorophyll molecules in this structure were destabilized and their optical and functional characteristics changed. Similar effects were also observed in cyanobacterial complexes of PSI. In addition, the antiseptic affected the rate of establishment of the equilibrium distribution of excited states by spectral forms in the PSI antenna complex. A significant effect of octenidine on the electron transfer rate in the PS I complex was also found: in its presence, the recombination of photo-separated charges between the photoactive pigment P700 and the terminal acceptor F_A/F_B occurred twice as fast.

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来源期刊

Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.