A Bioluminescent Test System Based on Recombinant L. mingrelica Firefly Luciferase as a Means of Investigating the Efficacy of the Gentamicin Effect on Live E. coli Cells
IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology
{"title":"A Bioluminescent Test System Based on Recombinant L. mingrelica Firefly Luciferase as a Means of Investigating the Efficacy of the Gentamicin Effect on Live E. coli Cells","authors":"G. Yu. Lomakina, S. S. Kaminskaya, N. N. Ugarova","doi":"10.1134/S0006350924700556","DOIUrl":null,"url":null,"abstract":"<div><p>The possibilities of using a test system based on live <i>E. coli</i> BL-21 (DE3) Codon Plus cells expressing pH-resistant <i>Luciola mingrelica</i> firefly luciferase to study the kinetics of the effect of aminoglycosides (using the example of gentamicin) on the content of ATP and luciferase inside and outside the cells were demonstrated. It has been shown that after 3 h of incubation of bacteria with an antibiotic it was possible to assess changes in cell viability and the effectiveness of the antibiotic, as well as to predict the formation of persisters. The method is promising for conducting rapid primary high-performance screening of antibacterial agents and drug forms to assess their effectiveness and the mechanism of action.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 3","pages":"477 - 484"},"PeriodicalIF":4.0330,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biophysics","FirstCategoryId":"4","ListUrlMain":"https://link.springer.com/article/10.1134/S0006350924700556","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
The possibilities of using a test system based on live E. coli BL-21 (DE3) Codon Plus cells expressing pH-resistant Luciola mingrelica firefly luciferase to study the kinetics of the effect of aminoglycosides (using the example of gentamicin) on the content of ATP and luciferase inside and outside the cells were demonstrated. It has been shown that after 3 h of incubation of bacteria with an antibiotic it was possible to assess changes in cell viability and the effectiveness of the antibiotic, as well as to predict the formation of persisters. The method is promising for conducting rapid primary high-performance screening of antibacterial agents and drug forms to assess their effectiveness and the mechanism of action.
BiophysicsBiochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍：
Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
