用数学方法评估天线异质性和光系统 II 氧发生复合物的活性

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology

Biophysics Pub Date : 2024-12-20 DOI:10.1134/S0006350924700490

N. S. Degtereva, T. Yu. Plyusnina, S. S. Khrushchev, R. N. Chervitsov, E. N. Voronova, O. V. Yakovleva, T. K. Antal, G. Yu. Riznichenko, A. B. Rubin

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引用次数: 0

摘要

光系统II是光合作用的主要色素-蛋白复合物之一，对不利环境因子高度敏感。光系统II特性的异质性是自养生物抵抗逆境因子的必要条件。光系统II非均质性评价可用于环境监测，实现环境污染的快速检测。提出了一种评估光系统II异质性的综合方法；利用数学模型和JIP测试参数对3-（3,4-二氯苯基）-1,1-二甲基尿素处理过的样品的叶绿素a荧光诱导曲线形状进行了数学分析。在8和16 W/ m-2的光照下，对小球藻、场景藻、弓形虫、胸膜绿藻和Stichococcus细胞培养物进行荧光诱导曲线分析。对不同天线尺寸的反应中心（α中心和β中心）的比例进行了估计，并确定了活性和非活性析氧配合物的比例。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Assessment of Antenna Heterogeneity and the Activity of the Oxygen-Evolving Complex of Photosystem II Using Mathematical Methods

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Assessment of Antenna Heterogeneity and the Activity of the Oxygen-Evolving Complex of Photosystem II Using Mathematical Methods

Photosystem II is one of the main pigment–protein complexes of photosynthesis, which is highly sensitive to adverse environmental factors. The heterogeneity of photosystem II properties is necessary for the resistance of autotrophic organisms to stress factors. The assessment of photosystem II heterogeneity can be used in environmental monitoring for the rapid detection of environmental pollution. A comprehensive approach to the assessment of photosystem II heterogeneity is proposed; it is based on a mathematical analysis of the shape of the chlorophyll a fluorescence induction curve of samples treated with 3-(3,4-dichlorophenyl)-1,1-dimethyl urea using a mathematical model and JIP test parameters. Fluorescence induction curves obtained for treated samples of Chlorella, Scenedesmus, Ankistrodesmus, Pleurochloris, and Stichococcus cell cultures were analyzed that grew in light with an intensity of 8 and 16 W/m^–2. For all cases, the ratio of reaction centers with different antenna sizes (alpha and beta centers) was estimated and the proportion of active and inactive oxygen-evolving complexes was determined.

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来源期刊

Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.