V. V. Malysheva, L. V. Stepanova, A. M. Vyshedko, L. V. Bel’skaya, E. A. Sarf, Z. Khalzhanova, O. A. Kolenchukova, V. A. Kratasyuk
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引用次数: 0
Abstract—The saliva of athletes with anaerobic (track and field athletes) and aerobic (swimmers) physical loads was studied. Saliva was collected before (in a state of physical rest) and after training. It was found that physical load significantly increased the concentration of total protein in saliva and the activity of hydrogen ions (pH) in the saliva. Catalase activity in saliva was significantly increased only after aerobic physical load, as opposed to anaerobic load. The bioluminescent saliva index was significantly increased for athletes engaged in aerobic physical load compared to that for an anaerobic load. Saliva biomarkers such as the concentration of lactate and total protein, catalase activity, concentration of calcium, potassium, and magnesium ions, as well as the content of group IV amides, had a significant effect on the enhancement of light emission in a bioluminescent two-enzyme reaction. The content of group IV amides and a complex of oligosaccharides, polysaccharides, phosphatase, and phospholipids in the saliva of athletes engaged in anaerobic physical load could more strongly inhibit bioluminescent glow than the concentration of triene conjugates and the content of group II amides in the saliva of athletes engaged in aerobic physical load. Thus, significant biomarkers of the physiological state of the body have been identified in the saliva of athletes engaged in aerobic or anaerobic physical loads, which were able to affect the integral response of a bioluminescent enzymatic biotest. A change in the integral bioluminescent saliva index, depending on the type of physical load for an athlete, can be used in sports medicine to prevent physical overloads.
摘要 研究了无氧运动（田径运动员）和有氧运动（游泳运动员）运动员的唾液。在训练前（身体休息状态）和训练后采集唾液。结果发现，体力负荷会明显增加唾液中总蛋白质的浓度和唾液中氢离子的活性（pH 值）。只有在有氧运动负荷后，唾液中过氧化氢酶的活性才会明显增加，而无氧运动负荷则相反。与无氧负荷相比，有氧负荷运动员的唾液生物发光指数明显增加。唾液中的生物标志物，如乳酸盐和总蛋白质的浓度、过氧化氢酶的活性、钙、钾和镁离子的浓度以及第 IV 组酰胺的含量，对生物发光双酶反应中光发射的增强有明显的影响。与从事有氧运动的运动员唾液中三烯共轭物的浓度和 II 族酰胺的含量相比，从事无氧运动的运动员唾液中 IV 族酰胺的含量和由低聚糖、多糖、磷酸酶和磷脂组成的复合物对生物发光的抑制作用更强。因此，在进行有氧或无氧体力负荷的运动员的唾液中发现了人体生理状态的重要生物标志物，它们能够影响生物发光酶生物测试的整体反应。生物发光唾液综合指数的变化取决于运动员的身体负荷类型，可用于运动医学，以防止身体超负荷。
BiophysicsBiochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍：
Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.
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