Biophysics最新文献_第7页

The Nature of Intermolecular Interactions Affecting Oligomerization of Nt.BspD6I Nickase

IF 4.033

Biophysics Pub Date : 2025-02-26 DOI: 10.1134/S0006350924700672

V. N. Antipova, A. K. Yunusova, R. I. Artyukh

引用次数: 0

Adrenergic Regulation of Myocardial and Vascular Function in Hypothermia

IF 4.033

Biophysics Pub Date : 2025-02-26 DOI: 10.1134/S0006350924700805

M. H. Galimova, A. S. Averin

{"title":"Adrenergic Regulation of Myocardial and Vascular Function in Hypothermia","authors":"M. H. Galimova, A. S. Averin","doi":"10.1134/S0006350924700805","DOIUrl":"10.1134/S0006350924700805","url":null,"abstract":"<div><p>Regulation of the cardiovascular system in hypothermia and after its exposure is one of the important and urgent issues of biomedical research. This is due both to the severe consequences of hypothermia and to the potential benefits of its use for medical purposes. Adrenergic regulation is key for the normal functioning of the cardiovascular system. There are three groups of adrenergic receptors, α1, α2, and β, which are expressed in different proportions in the myocardium and blood vessels. Activation of each of the subtypes of adrenoreceptors can cause multidirectional effects, which are largely modified at low temperatures. The effectiveness of β-adrenergic stimulation decreases and can even act unidirectionally with hypothermia, leading to disorders in the work of the myocardium. The severity of the effects of α1-adrenoagonists in the heart and blood vessels also decreases; however, sensitivity to stimulation of α1a receptors in blood vessels may increase. Activation of this type of receptor has a cardioprotective effect. Activation of α2-adrenergic receptors also causes a wide range of cardioprotective effects. At the same time, there is evidence of direct inotropic and vascular effects mediated by this type of receptor. There is some evidence of an increase in these effects in hypothermia. The present review considers the current state of research on the effects of stimulation of certain types of adrenoreceptors in normal and hypothermic conditions. The mechanisms of physiological effects and the prospects for their further research are discussed.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 4","pages":"720 - 728"},"PeriodicalIF":4.033,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143496688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Features of the Effects of Exposure to 90–170 MeV Proton Radiation on the Blood-Forming Organs in Mice under Total Irradiation with Proton Pencil Scanning Beam Depending on the Linear Energy Transfer of Particles

IF 4.033

Biophysics Pub Date : 2025-02-26 DOI: 10.1134/S0006350924700854

O. M. Rozanova, T. A. Belyakova, E. N. Smirnova, S. S. Sorokina, A. R. Dyukina, A. E. Shemyakov, N. S. Strelnikova

{"title":"Features of the Effects of Exposure to 90–170 MeV Proton Radiation on the Blood-Forming Organs in Mice under Total Irradiation with Proton Pencil Scanning Beam Depending on the Linear Energy Transfer of Particles","authors":"O. M. Rozanova, T. A. Belyakova, E. N. Smirnova, S. S. Sorokina, A. R. Dyukina, A. E. Shemyakov, N. S. Strelnikova","doi":"10.1134/S0006350924700854","DOIUrl":"10.1134/S0006350924700854","url":null,"abstract":"<div><p>The effect of proton radiation on the induction of cytogenetic damage in the bone marrow, the production of reactive oxygen species in whole blood, and the state of the thymus and spleen depending on linear energy transfer were studied during total irradiation of mice before the Bragg peak and at the peak in the dose range 0.1–1.5 Gy. It was found that the yield of polychromatophilic erythrocytes with micronuclei at all doses of proton radiation at the Bragg peak with a linear energy transfer of 2.5 keV/μm was close to the level of linear energy transfer with micronuclei for the corresponding doses of X-ray radiation with a linear energy transfer of 2.0 keV/μm; while when irradiated before the Bragg peak with a linear energy transfer of 0.7 keV/μm, the level of cytogenetic damage was significantly lower. The coefficient of relative biological effectiveness of proton irradiation calculated from the linear energy transfer by estimating micronuclei frequency at the Bragg peak was 1.15, and before the peak it was 0.63. Organ-specific differences in the patterns of pathophysiological effects were revealed, depending on the dose and linear energy transfer of protons on the thymus and spleen of mice, as well on the state of the antioxidant system of blood cells.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 4","pages":"775 - 783"},"PeriodicalIF":4.033,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143496747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Synchronizing the Human Heart Rhythm with Geomagnetic Field Variations: Are There Particular Frequencies?

IF 4.033

Biophysics Pub Date : 2025-02-26 DOI: 10.1134/S0006350924700878

T. A. Zenchenko, N. I. Khorseva, A. A. Stankevich

{"title":"The Effect of Synchronizing the Human Heart Rhythm with Geomagnetic Field Variations: Are There Particular Frequencies?","authors":"T. A. Zenchenko, N. I. Khorseva, A. A. Stankevich","doi":"10.1134/S0006350924700878","DOIUrl":"10.1134/S0006350924700878","url":null,"abstract":"<div><p>Studies of the phenomenology of the effect of synchronization of heart rate fluctuations in a person at rest with variations of the geomagnetic field in the range of 3–40 min periods have been continued. During 2012–2023, 508 experiments (lasting 100–120 min each) were conducted to monitor minute heart rate indicators in three practically healthy women (55, 45 and 30 years old). Individual experimental samples comprised 328, 113, and 67 records, respectively. It was shown that for each of the three volunteers, in about 60% of the experiments, the wavelet spectrum of heart rates turned out to be very close to the spectrum of synchronous variations of at least one of the horizontal components of the geomagnetic field (<i>X</i> or <i>Y</i>). As well, within the studied frequency range of 3–40 min, three sub-ranges were identified in which the degree of synchronicity of oscillation occurrence was maximum: 3.5 min, 10–12 min, and 33–36 min. It can be concluded that the effect of biogeosynchronization apparently did not occur uniformly over the entire range of 3–40 min, but mainly in these sub-ranges.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 4","pages":"792 - 802"},"PeriodicalIF":4.033,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143496749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of TRO19622 (Olesoxime) on the Functional Activity of Isolated Mitochondria and Cell Viability

IF 4.033

Biophysics Pub Date : 2025-02-26 DOI: 10.1134/S0006350924700714

A. I. Ilzorkina, N. V. Belosludtseva, A. A. Semenova, M. V. Dubinin, K. N. Belosludtsev

{"title":"The Effect of TRO19622 (Olesoxime) on the Functional Activity of Isolated Mitochondria and Cell Viability","authors":"A. I. Ilzorkina, N. V. Belosludtseva, A. A. Semenova, M. V. Dubinin, K. N. Belosludtsev","doi":"10.1134/S0006350924700714","DOIUrl":"10.1134/S0006350924700714","url":null,"abstract":"<div><p>TRO19622 (olesoxime, a cholesterol-like cytoprotector) is an experimental drug developed for the potential therapy of a number of incurable degenerative diseases. The main molecular target of this compound in the cell is the porin proteins of the outer mitochondrial membrane, which play a key role in regulating the exchange of metabolites between mitochondria and the rest of the cell. Violations of the activity of these channels can cause the development of mitochondrial dysfunction in healthy cells. This paper evaluates the main indicators of mitochondrial functions and the cell viability index in cultures after their incubation with TRO19622. It was found that TRO19622 at concentrations of 15–30 μM inhibited the rates of phosphorylating and uncoupled respiration of isolated mitochondria (states 3 and 3UDNP) with succinate as a substrate, but did not affect the enzymatic activity of complexes I–IV of the respiratory chain. It was shown that TRO19622 in the studied doses did not affect the rate of H<sub>2</sub>O<sub>2</sub> formation in mitochondria and the parameter of calcium capacity, reflecting the resistance of organelles to the opening of a calcium-dependent nonspecific pore. Incubation of human skin fibroblasts and breast adenocarcinoma cells (MCF-7) with 30 μM TRO19622 for 48 h had no effect on the production of reactive oxygen species and cell viability. The mechanisms of action of TRO19622 on the oxidative phosphorylation system and the prospects of using this mitochondrial-directed therapeutic agent are discussed.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 4","pages":"630 - 638"},"PeriodicalIF":4.033,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143496714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioluminescence Enzymatic Bioassay of Saliva for Occupational Monitoring of the Functional State of the Body in Rail Transportation Workers 唾液生物发光酶生物测定法用于轨道交通工人身体功能状态的职业监测

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S0006350924700647

L. V. Stepanova, O. A. Kolenchukova, G. V. Zhukova, O. S. Sutormin, V. A. Kratasyuk

{"title":"Bioluminescence Enzymatic Bioassay of Saliva for Occupational Monitoring of the Functional State of the Body in Rail Transportation Workers","authors":"L. V. Stepanova, O. A. Kolenchukova, G. V. Zhukova, O. S. Sutormin, V. A. Kratasyuk","doi":"10.1134/S0006350924700647","DOIUrl":"10.1134/S0006350924700647","url":null,"abstract":"<div><p>A bioluminescence enzymatic assay of saliva was proposed as a noninvasive method to monitor the functional state of the body. The luminescence intensity from coupled enzymatic reactions catalyzed by NADH:FMN oxidoreductase and luciferase on exposure to saliva served as an indicator of the body state of workers at work. The absence of a significant difference between bioluminescence values measured before and after a shift with no changes in biochemical, physical, and chemical parameters of saliva suggested adaptation to a workload. An increased bioluminescence intensity was typical for workers in a state of chronic fatigue; a decreased intensity was observed in workers with chronic diseases and harmful habits. Bioluminescence intensity was found to depend on the concentrations of lactate and lipid peroxidation products, the ionic and mineral composition, and parameters of free radical oxidation and antiradical protection. Thus, the dependence of the saliva constituents on the life conditions and health status of workers can be detected using the bioluminescence enzymatic bioassay, which is suitable for rapid occupational monitoring.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 3","pages":"575 - 583"},"PeriodicalIF":4.033,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142859640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Measurements of Photochemical Reflectance Index as a Tool for Remote Monitoring of Photosynthetic Parameters of Plants 光化学反射率的测量作为植物光合参数远程监测的工具

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S0006350924700581

Yu. A. Zolin, E. M. Sukhova, V. S. Sukhov

{"title":"Measurements of Photochemical Reflectance Index as a Tool for Remote Monitoring of Photosynthetic Parameters of Plants","authors":"Yu. A. Zolin, E. M. Sukhova, V. S. Sukhov","doi":"10.1134/S0006350924700581","DOIUrl":"10.1134/S0006350924700581","url":null,"abstract":"<div><p>Developing remote and proximal sensing techniques to detect the early photosynthetic responses in stress conditions is of importance for agriculture and ecology. The photochemical reflectance index (PRI), which is typically calculated from the reflected light intensities at 531 and 570 nm, is potentially sensitive to rapid changes in photosynthesis under unfavorable conditions. Mechanisms of changes in PRI are thought to include chloroplast shrinkage, aggregation of light-harvesting complexes, transitions in the xanthophyll cycle, and changes in chlorophyll and carotenoid concentrations. This renders it difficult to employ PRI difficult in monitoring plant health. Use of pulsed light for measurements, study of the light-induced changes in PRI, and analysis of modified PRIs provide means to improve the efficiency of PRI applications. Other means (e.g., developing methods to estimate PRI on the basis of RGB imaging) are also impossible to exclude. Improving techniques to measure and analyze PRI holds significant promise for monitoring the photosynthetic responses of plants to environmental stress factors.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 3","pages":"512 - 521"},"PeriodicalIF":4.033,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142859641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prolonged Luminescence Kinetics of Erythrosine in the Breast Tissue in vitro 红霉素在乳腺组织中的体外长时间发光动力学

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S0006350924700611

S. N. Letuta, A. T. Ishemgulov, M. A. Senchukova

引用次数: 0

Antioxidant Properties of Plant Plastoquinone in Vivo and in Vitro 植物质体醌体内外抗氧化性能的研究

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S0006350924700520

D. V. Vetoshkina, A. A. Nikolaev, M. M. Borisova-Mubarakshina

{"title":"Antioxidant Properties of Plant Plastoquinone in Vivo and in Vitro","authors":"D. V. Vetoshkina, A. A. Nikolaev, M. M. Borisova-Mubarakshina","doi":"10.1134/S0006350924700520","DOIUrl":"10.1134/S0006350924700520","url":null,"abstract":"<div><p>Plastoquinone (PQ) is a mobile electron carrier from photosystem II to the cytochrome <i>b</i><sub>6</sub>/<i>f</i> complex in the photosynthetic electron transport chain (PETC). In addition, PQ acts together with many other isoprenoids to perform the antioxidant function and to protect plant cells from reactive oxygen species (ROS). The review describes the PQ reactions with ROS, including singlet oxygen, superoxide anion radical, and hydrogen peroxide, in chloroplasts of higher plants. Data from many studies on the effect of various stress factors on PQ biosynthesis were analyzed to demonstrate that the PQ content increases in almost all stress conditions. An increase in PQ biosynthesis is therefore a factor that contributes to the plant viability by protecting the photosynthetic apparatus from oxidative degradation. A special section describes the methods to create plants with a higher PQ level and to assess their resistance to environmental factors. Applications of plant PQ as a protector of membrane structures from oxidation are discussed on the basis of its high antioxidant activity and the capability of diffusing in the lipid phase.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 3","pages":"445 - 458"},"PeriodicalIF":4.033,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142859599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Unique Features of the Luminescent Fungus Mycena gombakensis gombakensis发光真菌的独特特征

IF 4.033

Biophysics Pub Date : 2024-12-20 DOI: 10.1134/S000635092470057X

A. P. Puzyr, E. D. Posokhina, A. A. Timofeev, A.E. Burov, S. E. Medvedeva, I. N. Pavlov

引用次数: 0