Bioluminescence Enzymatic Bioassay of Saliva for Occupational Monitoring of the Functional State of the Body in Rail Transportation Workers

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology

Biophysics Pub Date : 2024-12-20 DOI:10.1134/S0006350924700647

L. V. Stepanova, O. A. Kolenchukova, G. V. Zhukova, O. S. Sutormin, V. A. Kratasyuk

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Abstract

A bioluminescence enzymatic assay of saliva was proposed as a noninvasive method to monitor the functional state of the body. The luminescence intensity from coupled enzymatic reactions catalyzed by NADH:FMN oxidoreductase and luciferase on exposure to saliva served as an indicator of the body state of workers at work. The absence of a significant difference between bioluminescence values measured before and after a shift with no changes in biochemical, physical, and chemical parameters of saliva suggested adaptation to a workload. An increased bioluminescence intensity was typical for workers in a state of chronic fatigue; a decreased intensity was observed in workers with chronic diseases and harmful habits. Bioluminescence intensity was found to depend on the concentrations of lactate and lipid peroxidation products, the ionic and mineral composition, and parameters of free radical oxidation and antiradical protection. Thus, the dependence of the saliva constituents on the life conditions and health status of workers can be detected using the bioluminescence enzymatic bioassay, which is suitable for rapid occupational monitoring.

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来源期刊

Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics

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1.20

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0.00%

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期刊介绍： Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.