Biophysics最新文献_第6页

Effect of the Normacor and Gelofusine Combination on the Preservation of an Isolated Rat Heart during Gas Hypothermic Storage under a Hyperbaric Mixture of Carbon Monoxide and Oxygen Normacor和Gelofusine联合使用对大鼠离体心脏在一氧化碳和氧气高压混合物下低温保存的影响

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925701179

A. E. Gurin, E. L. Gagarinsky, E. R. Valeeva, E. E. Fesenko Jr.

{"title":"Effect of the Normacor and Gelofusine Combination on the Preservation of an Isolated Rat Heart during Gas Hypothermic Storage under a Hyperbaric Mixture of Carbon Monoxide and Oxygen","authors":"A. E. Gurin, E. L. Gagarinsky, E. R. Valeeva, E. E. Fesenko Jr.","doi":"10.1134/S0006350925701179","DOIUrl":"10.1134/S0006350925701179","url":null,"abstract":"One of the key problems of transplantation is the short period of safe hypothermic storage of a donor heart. A promising method of its prolongation is gas hypothermic preservation in mixtures of oxygen with carbon monoxide and other gas agents. However, the standardization of this approach requires the selection of conditions for cardiac arrest and washing it of blood before gas exposure. The aim of this work was to test the possibility of using the method of hyperbaric gas hypothermic preservation of the rat heart in combination with the use of Normacor cardioplegic solution mixed with the blood plasma substitute Gelofusine. An experimental study was conducted on isolated rat hearts (n = 30). Functional (contractility and heart rate) and morphological parameters were evaluated after 12 and 24 h of storage at 4°C and 6.5 atm pressure of a CO : O2 (1 : 1) gas mixture. Three preparation protocols were compared: perfusion with Normacor® solution + Gelofusine at 37°C, perfusion with Custodiol solution at 4°C and storage without perfusion. It was shown that the combination Normakor® + Gelofusine ensures stable restoration of the contractile function of the heart (~50% of the native control) and its morphological preservation after 12 and 24 h of storage. After 12 h of storage, the “Normacor” group showed significantly better functional heart recovery results compared to the “Custodiol” group. Histological analysis revealed moderate and potentially reversible ischemic reperfusion injuries in all groups after 24 h of exposure to a gaseous medium. It was concluded that the combination of Normacor and Gelofusine solutions registered in Russia is an effective means of pretreatment of an organ for subsequent gas hypothermic preservation, which may contribute to the introduction of this approach into clinical practice to extend the shelf life of a donor heart.","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 6","pages":"1082 - 1089"},"PeriodicalIF":4.033,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Pro- and Antioxidant Properties of Compositions Based on Endogenous Choline Derivatives 内源性胆碱衍生物组成物的亲氧化性和抗氧化性

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S000635092570099X

D. A. Krugovov, I. F. Rusina, N. V. Zakhvatova, Yu. N. Egorova, L. A. Smurova, V. V. Vedutenko, V. G. Kondratovich, M. P. Berezin, O. T. Kasaikina

{"title":"Pro- and Antioxidant Properties of Compositions Based on Endogenous Choline Derivatives","authors":"D. A. Krugovov, I. F. Rusina, N. V. Zakhvatova, Yu. N. Egorova, L. A. Smurova, V. V. Vedutenko, V. G. Kondratovich, M. P. Berezin, O. T. Kasaikina","doi":"10.1134/S000635092570099X","DOIUrl":"10.1134/S000635092570099X","url":null,"abstract":"The effect of endogenous quaternary ammonium compounds, derivatives of choline, acetylcholine, and L-carnitine, on the decomposition of hydroperoxides has been studied. It has been established that choline and acetylcholine in organic media form joint nanoaggregates {QAC–ROOH}, in which the radical decomposition of hydroperoxides is accelerated; choline and acetylcholine immobilized on a solid carrier (microcrystalline cellulose) retain the ability to generate radicals in the presence of hydroperoxides and initiate chain processes of oxidation and radical polymerization. L-Carnitine, unlike acetylcholine and choline, is an internal salt, and the R4N+ cation is neutralized by the carboxyl group –({text{CO}}_{2}^{ - }), that is, L-carnitine does not have an external counterion and, probably for this reason, differs from acetylcholine and choline in the mechanisms of adsorption and interaction with hydroperoxides. Individual L-carnitine does not affect lipid oxidation processes, but in combination with 2-ethyl-6-methyl-3-hydroxypyridine forms 2-ethyl-6-methyl-3-hydroxypyridinium carnitinate , which has antioxidant and biological activity. In this work, choline, L-carnitine, and 2-ethyl-6-methyl-3-hydroxypyridinium carnitinate are compared in terms of growth-stimulating activity using the example of wheat seed germination.","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 6","pages":"923 - 929"},"PeriodicalIF":4.033,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Collagen Peptides Made of Pork Hyaline Cartilages for Nutritiology and Biomedicine 猪肉透明软骨胶原蛋白多肽的营养和生物医学研究

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925700939

T. I. Nikolaeva, T. N. Pachovkin, E. V. Grishina, K. S. Laurinavichu, P. V. Shekhovtsov

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The Radioprotective Effect of Extremely High Frequency Electromagnetic Radiation in vitro: Dependence on Parameters and Exposure Conditions 极高频电磁辐射的体外辐射防护作用：对参数和暴露条件的依赖

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S000635092570112X

A. B. Gapeyev, N. A. Lukyanova, A. V. Bakanov, T. P. Kulagina

{"title":"The Radioprotective Effect of Extremely High Frequency Electromagnetic Radiation in vitro: Dependence on Parameters and Exposure Conditions","authors":"A. B. Gapeyev, N. A. Lukyanova, A. V. Bakanov, T. P. Kulagina","doi":"10.1134/S000635092570112X","DOIUrl":"10.1134/S000635092570112X","url":null,"abstract":"In modern conditions, open and self-organizing biological systems are exposed to a complex spectrum of multi-frequency and modulated electromagnetic radiation of various frequency ranges. This work is devoted to the study of the effect of X-ray irradiation at a dose of 4 Gy on the level of DNA damage in peripheral blood leukocytes of mice pre-exposed to extremely high frequency electromagnetic radiation (EHF EMR) in vitro depending on the carrier and modulating frequencies, exposure duration, intensity and duty cycle of the modulating signal. Using the DNA comet method, it was found that pre-irradiation of cells with electromagnetic radiation of extremely high frequencies leads to a statistically significant decrease in radiation damage to DNA during pulse modulation of radiation with frequencies of 0.1, 1, 16, 32, and 50 Hz. At the same time, irradiation both in the continuous generation mode and in the case of amplitude modulation by a harmonic signal was ineffective. The greatest radioprotective effect was observed at carrier frequencies of 42.2, 51.8, and 61.22 GHz, an exposure duration of 20–30 min, an incident power density of 100–250 μW/cm2, and a duty cycle of the modulation pulse signal of 2–4. The mechanisms of implementation of the obtained biological effects of extremely high frequency electromagnetic radiation associated with the induction of free radicals are discussed.","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 6","pages":"1033 - 1041"},"PeriodicalIF":4.033,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Modification of the Extracellular Matrix of the Umbilical Cord Mesenchymal Stromal Cells during Long-Term Simulating of Microgravity Effects 长期模拟微重力作用时脐带间充质间质细胞外基质的修饰

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925700873

A. N. Gornostaeva, Yu. A. Romanov, L. B. Buravkova

{"title":"Modification of the Extracellular Matrix of the Umbilical Cord Mesenchymal Stromal Cells during Long-Term Simulating of Microgravity Effects","authors":"A. N. Gornostaeva, Yu. A. Romanov, L. B. Buravkova","doi":"10.1134/S0006350925700873","DOIUrl":"10.1134/S0006350925700873","url":null,"abstract":"<div>It is known that microgravity leads to significant changes in the functioning of human physiological systems. In vitro, mechanosensitive cells also adapt to microgravity, demonstrating a rearrangement of cytoskeletal elements and functional activity. The effect of prolonged microgravity simulated on a Gravite position randomization device was studied on cultured multipotent mesenchymal stromal cells of umbilical cord tissue. After 21 days of exposure, the cells retained high viability and a characteristic stromal phenotype. The expression of CD90 and CD105 markers involved in cell–cell and cell–matrix adhesion increased on their surface. The cytokine profile changed, and the concentration of pleiotropic cytokines MCP-3, GM-CSF, and PDGF-AA, which potentiate metalloproteinase activity, increased. The expression of genes encoding matrix metalloproteinase MMP-1 and osteocalcin increased, while osteopontin decreased. The main extracellular matrix proteins, fibronectin, collagen, and osteopontin were visualized in both experimental groups, while collagen was more pronounced after microgravity. The described changes indicate adaptive shift in the local microenvironment and remodeling of the extracellular matrix in response to prolonged exposure to simulated microgravity while maintaining the functional activity of mesenchymal stromal cells.</div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 5","pages":"816 - 824"},"PeriodicalIF":4.033,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Comparative Analysis of Sensitizing Activity and Biodistribution Characteristics of Chlorin e6 Derivatives: Effect of the Nature of Charged Groups 氯e6衍生物致敏活性及生物分布特性的比较分析：带电基团性质的影响

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925700836

T. E. Zorina, T. I. Ermilova, I. V. Kablov, I. E. Kravchenko, T. V. Shman, T. V. Kustova, D. B. Berezin, I. S. Khudyaeva, V. P. Zorin

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Biogeochemical Engineering Technologies for Creating Artificial Soils 人造土壤的生物地球化学工程技术

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925701088

V. N. Bashkin, A. O. Alekseev

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Normalization of Ca2+ Homeostasis Does Not Lead to Correction of Intracellular pH in Hippocampal Culture Neurons under the Influence of Ammonium Ions 在铵离子的影响下，Ca2+稳态的正常化不会导致海马培养神经元细胞内pH的纠正

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925701052

A. I. Sergeev, P. V. Nekrasov

{"title":"Normalization of Ca2+ Homeostasis Does Not Lead to Correction of Intracellular pH in Hippocampal Culture Neurons under the Influence of Ammonium Ions","authors":"A. I. Sergeev, P. V. Nekrasov","doi":"10.1134/S0006350925701052","DOIUrl":"10.1134/S0006350925701052","url":null,"abstract":"In pathologies associated with excitotoxicity and impaired calcium homeostasis hyperexcitation of neurons is often observed, including during ischemic stroke, hyperammonemia, and neurodegenerative diseases. In the case of long-term sustained depolarization, this hyperactivity disrupts many processes, including the energy balance of ATP, and can lead to cell death. These reactions are often accompanied by prolonged acidification of the cytosol. This affects the activity of a large number of ion channels dependent on intracellular pH, other molecular objects that regulate the concentration of [Ca2+]i, and other ions in the cell, as well as the membrane potential. Such processes may additionally lead to hyperexcitation and disruption of ion homeostasis. Understanding the relationship between [Ca2+]i and pHi is important for the development of methods to eliminate these dysfunctions. In particular, it remains unclear whether correction of Ca2+ homeostasis entails normalization of pHi. In this work, it was shown that the elimination of hyperexcitation manifestations, namely, the ultra-high frequency of [Ca2+]i pulses and the elevated level of basal [Ca2+]i by using the dopamine D2-like receptor (D2-like) agonist PD-168077 (10 μM) or the α2-adrenergic receptor agonist guanfacine (10 μM) does not lead to a noticeable correction of pHi in hippocampal neurons and astrocytes. Thus, to eliminate the effects of hyperexcitation, pHi regulation is a separate task, independent of approaches to normalize calcium homeostasis.","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 6","pages":"977 - 985"},"PeriodicalIF":4.033,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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The Effect of Inflammatory Activation on Mitochondrial Function and Expression of Apoptosis Inhibitor Genes in THP-1 Acute Myeloid Leukemia Cells 炎症活化对THP-1急性髓系白血病细胞线粒体功能及凋亡抑制基因表达的影响

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925701076

E. I. Meshcheriakova, M. I. Kobyakova, I. V. Odinokova, A. V. Kuzovlev, I. S. Fadeeva, R. S. Fadeev

{"title":"The Effect of Inflammatory Activation on Mitochondrial Function and Expression of Apoptosis Inhibitor Genes in THP-1 Acute Myeloid Leukemia Cells","authors":"E. I. Meshcheriakova, M. I. Kobyakova, I. V. Odinokova, A. V. Kuzovlev, I. S. Fadeeva, R. S. Fadeev","doi":"10.1134/S0006350925701076","DOIUrl":"10.1134/S0006350925701076","url":null,"abstract":"A study was conducted on the key functions of mitochondrial respiration and the expression of genes of the BCL2 and IAP families in THP-1 cells of acute myeloid leukemia upon lipopolysaccharide and zymosan A-induced inflammatory activation, as well as under conditions of aseptic inflammatory activation simulating the inflammatory microenvironment using three-dimensional cell cultures. It was found that under conditions of both lipopolysaccharide and zymosan A-induced as well as aseptic inflammatory activation, a decrease in mitochondrial membrane potential and the rate of oxygen consumption in mitochondria occurred along with increased secretion of proinflammatory mediators IL-1β, TNF-α and IL-6. In addition, an increase in the production of reactive oxygen species and lactate was shown. It was also shown that in THP-1 cells, under conditions of inflammatory activation, there was an increase in the expression of the BIRC3, BIRC7, BCL2, BCL2L1, BCL2L2, and BCL2A1 genes encoding the corresponding antiapoptotic proteins.","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 6","pages":"993 - 1000"},"PeriodicalIF":4.033,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Ultrasound in the 0.88–8.00 MHz Range on the Cracking Processes of Frozen Cryoprotective Solutions When Cooled to –196°C 0.88-8.00 MHz范围内超声波对冷冻冷冻保护液冷却至-196℃时开裂过程的影响

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925701106

A. A. Andreev, D. G. Sadikova, T. N. Pashovkin

{"title":"The Effect of Ultrasound in the 0.88–8.00 MHz Range on the Cracking Processes of Frozen Cryoprotective Solutions When Cooled to –196°C","authors":"A. A. Andreev, D. G. Sadikova, T. N. Pashovkin","doi":"10.1134/S0006350925701106","DOIUrl":"10.1134/S0006350925701106","url":null,"abstract":"During cryopreservation of biological material, the stage of formation of a solid phase (ice) during cooling of living cells and a cryoprotective mixture is of great importance. The formation of ice, along with other factors, significantly affects the survival of cells during the freezing–thawing process. Most researchers limit their studies to a temperature range of 0 to –80°C. This range is considered the most critical due to damage to biological material caused by intracellular and extracellular ice crystal formation. However, the processes that occur during further cooling of the frozen material to storage temperatures (–196°C) are poorly understood. When cooled below –100°C, thermomechanical stress in the frozen medium increases due to its compression, thus causing cracking. Exposure to ultrasound at the initial stage of freezing eliminated supercooling in all studied solutions and significantly altered the temperature curve. This study demonstrates the effect of ultrasound in the 0.88–8.00 MHz frequency range on the cracking of frozen cryoprotective solutions and the formation of ice microparticles. Freezing was performed in a thin 0.2-mm layer at cooling to –196°C. The shape and size of the ice microparticles were determined by cryomicroscopy. With increasing frequency, the degree of homogeneity (size and area) of the microparticles and the shape factor (degree of roundness) increased. It has been shown that ultrasound can effectively influence the formation of cracks in frozen cryoprotective media, especially in the high-frequency range. The greatest effect of increasing the degree of roundness of microparticles was observed in a medium containing saline solution, 10% dimethyl sulfoxide, and 10% egg yolk.","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 6","pages":"1022 - 1026"},"PeriodicalIF":4.033,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0