发布求助
文献互助 智能选刊 最新文献

Biophysics最新文献

筛选
英文 中文
A Decrease in the Background Production of Reactive Oxygen Species by Neutrophils after the Action of Hypomagnetic Field Is Not Accompanied by a Violation of Their Chemiluminescent Response to Respiratory Burst Activators 低磁场作用后中性粒细胞活性氧本底生成的减少并不伴有它们对呼吸爆发激活剂化学发光反应的失常
IF 4.033
Biophysics Pub Date : 2024-03-18 DOI: 10.1134/S0006350923060155
V. V. Novikov, E. V. Yablokova, I. A. Shaev, N. I. Novikova, E. E. Fesenko
{"title":"A Decrease in the Background Production of Reactive Oxygen Species by Neutrophils after the Action of Hypomagnetic Field Is Not Accompanied by a Violation of Their Chemiluminescent Response to Respiratory Burst Activators","authors":"V. V. Novikov,&nbsp;E. V. Yablokova,&nbsp;I. A. Shaev,&nbsp;N. I. Novikova,&nbsp;E. E. Fesenko","doi":"10.1134/S0006350923060155","DOIUrl":"10.1134/S0006350923060155","url":null,"abstract":"<p>It has been shown that a decrease in background production of reactive oxygen species in peritoneal neutrophils of mice after a short-term (40 min) stay in hypomagnetic conditions (residual field of ~10 nT) at physiological temperatures, detected by the method of lucigenin-dependent chemiluminescence, was not accompanied by a violation of the chemiluminescent response to respiratory burst activators of formylated peptide <i>N</i>-formyl–Met–Leu–Phe (fMLP) and forbol ether forbol-12-meristate-13-acetate (FMA). These results were obtained by activated chemiluminescence method using lucigenin and luminol and various combinations of activators for the production of reactive oxygen species (forbol-12-meristate-13-acetate and/or <i>N</i>-formyl–Met–Leu–Phe). The study, together with the previously obtained results, makes it possible to exclude the systems controlling the respiratory burst in neutrophils from the main targets and acceptors that react to short-term deprivation of a magnetic field.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"68 6","pages":"978 - 983"},"PeriodicalIF":4.033,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140172478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Meta-Analysis of Randomized Clinical Trials on the Speed of Pulse Wave Propagation 关于脉搏波传播速度的随机临床试验的元分析
IF 4.033
Biophysics Pub Date : 2024-03-18 DOI: 10.1134/S0006350923060088
B. G. Gharamyan
{"title":"Meta-Analysis of Randomized Clinical Trials on the Speed of Pulse Wave Propagation","authors":"B. G. Gharamyan","doi":"10.1134/S0006350923060088","DOIUrl":"10.1134/S0006350923060088","url":null,"abstract":"<p>A theoretical analysis of the literature data on the pulse-wave propagation velocity using a combination of electrocardiographic and photoplethysmographic signals was carried out. Its diagnostic value in medical practice lies in its use as a noninvasive method for measuring blood pressure, as well as for determining arterial stiffness. Some studies have shown a relationship between the velocity of pulse-wave propagation and risk factors for cardiovascular diseases. Pulse-wave propagation velocity is an early marker and predictor of cardiovascular risk in both people with normal blood pressure and people with hypertension; it is used as the “gold standard” in determining arterial stiffness; however, the issue of accuracy and automation of measuring the pulse-wave propagation velocity remains unresolved.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"68 6","pages":"1053 - 1065"},"PeriodicalIF":4.033,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140172479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Mechanism of the Cytotoxic Effect of Noble Metal Polyacrylates on Tumor Cells 贵金属聚丙烯酸酯对肿瘤细胞的细胞毒性作用机制
IF 4.033
Biophysics Pub Date : 2024-03-18 DOI: 10.1134/S0006350923060040
A. K. Chigasova, L. A. Ostrovskaya, D. B. Korman, N. V. Bluhterova
{"title":"The Mechanism of the Cytotoxic Effect of Noble Metal Polyacrylates on Tumor Cells","authors":"A. K. Chigasova,&nbsp;L. A. Ostrovskaya,&nbsp;D. B. Korman,&nbsp;N. V. Bluhterova","doi":"10.1134/S0006350923060040","DOIUrl":"10.1134/S0006350923060040","url":null,"abstract":"<p>A comparative study of the cytotoxic activity and mechanism of action of polyacrylates containing gold (aurumacryl) and silver (argacryl) against human tumor cells (MCF-7 cell culture) has been carried out. The high cytotoxic activity of the drugs has been shown. Argacryl was found to be 20 times more effective than aurumacryl. It was found that apoptosis was the dominant mechanism in the death of tumor cells induced by aurumacryl and argacryl. Differences in the effect of drugs on the DNA structure of tumor cells were revealed.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"68 6","pages":"984 - 995"},"PeriodicalIF":4.033,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140172663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Piezo Pulsometric Analysis of the Functional State of the Human Cardiovascular System 压电脉搏计对人体心血管系统功能状态的分析
IF 4.033
Biophysics Pub Date : 2024-03-18 DOI: 10.1134/S0006350923060131
V. P. Nesterov, A. I. Burdygin, K. B. Ivanov, K. V. Sobol, I. V. Shemarova
{"title":"Piezo Pulsometric Analysis of the Functional State of the Human Cardiovascular System","authors":"V. P. Nesterov,&nbsp;A. I. Burdygin,&nbsp;K. B. Ivanov,&nbsp;K. V. Sobol,&nbsp;I. V. Shemarova","doi":"10.1134/S0006350923060131","DOIUrl":"10.1134/S0006350923060131","url":null,"abstract":"<p>The article expounds, with examples, how to apply the computer method of arterial piezo pulsometry designed to provide a rapid noninvasive test for the functional state and the nature of autonomic regulation of the cardiovascular (CV) system in patients varying in age and health status. The involvement and role were assessed for the contractile component of left ventricular (LV) myocytes as a universal muscle effector that ensures both the basic pumping function of the heart and the implementation of the autonomic regulation of heart contractions via peripheral neurohumoral mechanisms that maintain the optimal level of cardiac hemodynamics. The analysis was found to enhance the predictive value of early noninvasive pulsometric diagnosis of the individual functional state and the nature of the regulation of the human CV system during ontogeny. This knowledge helps identifying the risk that may threaten the evolutionary ability of the CV system to maintain a dynamic balance, self-regulation, and adaptation to changing environmental conditions and is associated with distorted homeostasis of the body.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"68 6","pages":"1066 - 1075"},"PeriodicalIF":4.033,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140172483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
On Methodological Errors in Estimating the Initial Velocities of Enzyme-Catalyzed Reactions and Approaches to Their Correction: A Case Study with Lactate Dehydrogenase 论估算酶催化反应初始速度的方法误差及其纠正方法：乳酸脱氢酶案例研究
IF 4.033
Biophysics Pub Date : 2024-03-18 DOI: 10.1134/S0006350923060118
A. Yu. Lianguzov, N. M. Malygina, T. A. Petrova
{"title":"On Methodological Errors in Estimating the Initial Velocities of Enzyme-Catalyzed Reactions and Approaches to Their Correction: A Case Study with Lactate Dehydrogenase","authors":"A. Yu. Lianguzov,&nbsp;N. M. Malygina,&nbsp;T. A. Petrova","doi":"10.1134/S0006350923060118","DOIUrl":"10.1134/S0006350923060118","url":null,"abstract":"<p>A universal algorithm for calculating the initial velocities of enzymatic reactions at the “zero” moment of time according to the kinetic curve of substrate consumption or product accumulation during direct kinetic measurements of enzyme activity is presented. The study was performed using the example of a commercial lactate dehydrogenase preparation and a standard biochemical set of reagents. The proposed approach makes it possible to practically eliminate systematic measurement error, to determine the initial reaction velocity with equal accuracy regardless of the level of enzyme activity, to reduces the analysis time to 1 min, and to expand the scope of application of standard reagent kits for the study of samples of enzymes of different origin in solving a wide range of tasks. The algorithm cannot be applied when using conjugated enzyme systems in an analytical procedure, since in this case there is a long initial lag phase on the kinetic curves.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"68 6","pages":"924 - 928"},"PeriodicalIF":4.033,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140172476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome-Wide Association Analysis in Chickpea Landraces and Cultivars 鹰嘴豆地方品种和栽培品种的全基因组关联分析
IF 4.033
Biophysics Pub Date : 2024-03-18 DOI: 10.1134/S0006350923060076
M. A. Duk, A. A. Kanapin, M. P. Bankin, M. A. Vishnyakova, S. V. Bulyntsev, M. G. Samsonova
{"title":"Genome-Wide Association Analysis in Chickpea Landraces and Cultivars","authors":"M. A. Duk,&nbsp;A. A. Kanapin,&nbsp;M. P. Bankin,&nbsp;M. A. Vishnyakova,&nbsp;S. V. Bulyntsev,&nbsp;M. G. Samsonova","doi":"10.1134/S0006350923060076","DOIUrl":"10.1134/S0006350923060076","url":null,"abstract":"<p>The chickpea <i>Cicer arientinum</i> is an important leguminous crop and is especially widely grown in the Near East. Because chickpea is highly susceptible to fungal diseases, such as Ascochyta blight and Fusarium blight, in wet weather conditions, disease-resistant and early-ripening varieties are critically important to breed. Genome associations were studied in 171 samples of chickpea plants grown in two experimental stations in Krasnodar krai (Kuban experimental station) and Astrakhan (Astrakhan experimental station). Relationships with genes were assessed for 12 phenotypic traits and 3 hallmarks of resistance to the pathogens <i>Fusarium</i>, <i>Ascochyta</i>, and Noctuidae. Variants associated with different phenotypic traits were identified using genome-wide association study (GWAS) methods.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"68 6","pages":"952 - 963"},"PeriodicalIF":4.033,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140172661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Refinement of the Parameters of β-Turns Using Neutron Diffraction Data 利用中子衍射数据完善 β 旋转参数
IF 4.033
Biophysics Pub Date : 2024-03-18 DOI: 10.1134/S0006350923060106
A. A. Korobkov, A. A. Khurmuzakiy, N. G. Esipova, V. G. Tymanyan, A. A. Anashkina
{"title":"The Refinement of the Parameters of β-Turns Using Neutron Diffraction Data","authors":"A. A. Korobkov,&nbsp;A. A. Khurmuzakiy,&nbsp;N. G. Esipova,&nbsp;V. G. Tymanyan,&nbsp;A. A. Anashkina","doi":"10.1134/S0006350923060106","DOIUrl":"10.1134/S0006350923060106","url":null,"abstract":"<p>β-Bends are a difficult to interpret type of polypeptide chain backbone structure of globular proteins. β-Bends are usually classified according to the dihedral angles φ and ψ of amino acid residues <i>i</i> + 1 and <i>i</i> + 2. Ramachandran map analysis of amino acid residues <i>i</i> + 1 and <i>i</i> + 2 indicates the resulting conformational stresses in bending. This kind of conformation stresses can only be compensated by additional interactions, such as additional hydrogen bonds, whose geometry and energy compensates for the β-bending stress. Neutronography is the only available direct method for determining the position of hydrogen atoms in the structures of chemical compounds, including proteins. In this work, β-bends from 176 protein structures from PDB established by neutronography are studied. In these structures, 3733 β-bends were found using the <i>i</i> → <i>i</i> + 3 hydrogen bonding criterion. Using clustering by the magnitude of conformational angles, eight types of bends were newly identified. The magnitudes of conformational angles for each type of bend were determined. The hypothesis of additional hydrogen bonding to stabilize the bend was not confirmed, suggesting that the bending stress is compensated by other factors.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"68 6","pages":"945 - 951"},"PeriodicalIF":4.033,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140172399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Thermodynamic Model of the Formation of Protein Aggregates on a Matrix 基质上蛋白质聚集体形成的热力学模型
IF 4.033
Biophysics Pub Date : 2024-03-18 DOI: 10.1134/S0006350923060210
E. O. Vasilenko, S. A. Kozin, V. A. Mitkevich, A. S. Buchelnikov, Yu. D. Nechipurenko
{"title":"A Thermodynamic Model of the Formation of Protein Aggregates on a Matrix","authors":"E. O. Vasilenko,&nbsp;S. A. Kozin,&nbsp;V. A. Mitkevich,&nbsp;A. S. Buchelnikov,&nbsp;Yu. D. Nechipurenko","doi":"10.1134/S0006350923060210","DOIUrl":"10.1134/S0006350923060210","url":null,"abstract":"<p>The formation of pathological protein aggregates is associated with many neurodegenerative diseases. The aggregates can form by multilayer adsorption of ligands on a matrix in some cases; for example, the set of membrane protein receptors may act as a matrix. A thermodynamic model was developed to describe the formation of linear aggregates in which ligands are stacked. Cases where complexes of the same type or two different types form the stacks are considered. The model can be used to study pathological aggregation of various proteins and, primarily, amyloid β and its isoforms. The mean, variance, and entropy were calculated for a theoretical distributions of aggregate sizes. A comparison was made with other theoretical models of the formation of amyloid aggregates.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"68 6","pages":"934 - 944"},"PeriodicalIF":4.033,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140172665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lipase and Esterase Activity in Soils from Ancient Burial Vessels 古代墓葬土壤中的脂肪酶和酯酶活性
IF 4.033
Biophysics Pub Date : 2024-03-18 DOI: 10.1134/S000635092306009X
N. N. Kashirskaya, E. V. Chernysheva, S. N. Udaltsov, L. N. Plekhanova
{"title":"Lipase and Esterase Activity in Soils from Ancient Burial Vessels","authors":"N. N. Kashirskaya,&nbsp;E. V. Chernysheva,&nbsp;S. N. Udaltsov,&nbsp;L. N. Plekhanova","doi":"10.1134/S000635092306009X","DOIUrl":"10.1134/S000635092306009X","url":null,"abstract":"<p>The activity of lipolytic enzymes was evaluated by the microplate method using heteromolecular exchange in soils from the bottom of the burial ware, followed by cluster analysis. Groups with a contrasting ratio of C12–C18 lipases and C4–C8 esterases were identified in a sample including 57 burial pots aged 2000 to 3000 years. The total lipolytic activity of soil samples from ware with an estimated initial presence of fat-containing funeral food products was above 6% of the maximum value within the sample. The inhibition of esterases in the presence of high concentrations of fatty acids was reflected in extremely low relative esterase activity of the soil microbial community at maximum C18 and C12 lipase activity in two groups of pots. In three groups, esterase activity was observed at high relative activity of C16 lipases, average relative activity of C18 lipases, and extremely low activity of true lipases. In the group of pots with high total lipolytic activity (with the exception of certain extreme values of this indicator), the proportion of true lipase activity was 1.7–1.8 times higher than the proportion of esterase activity. The grouping of funeral dishes according to the ratio of lipase and esterase activity reflected the characteristics of funeral food, whereas the absolute values of enzyme activity among selected vessels with the highest total lipolytic activity were grouped according to the type of burial.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"68 6","pages":"996 - 1003"},"PeriodicalIF":4.033,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140172669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of the Relationship between Activity of the Reticular Formation of the Medulla Oblongata and the Respiratory and Cardiovascular Systems in Rats under Painful Stimulation 疼痛刺激下大鼠延髓网状结构的活动与呼吸系统和心血管系统的关系分析
IF 4.033
Biophysics Pub Date : 2024-03-18 DOI: 10.1134/S0006350923060064
O. E. Dick
{"title":"Analysis of the Relationship between Activity of the Reticular Formation of the Medulla Oblongata and the Respiratory and Cardiovascular Systems in Rats under Painful Stimulation","authors":"O. E. Dick","doi":"10.1134/S0006350923060064","DOIUrl":"10.1134/S0006350923060064","url":null,"abstract":"<p>Using a method based on averaging the conditional probabilities of recurrence intervals, a comparative analysis was performed to estimate the direction of coupling between different physiological rhythms (fluctuations in blood pressure, respiration, and neuronal activity in the reticular formation of the medulla oblongata) in two groups of anesthetized rats with or without painful stimulation. Different variants were observed for the directions of coupling between the rhythms. A statistically significant unidirectionality of coupling was detected with most of the data obtained from rats of the control group and rats with experimentally induced colitis. Painful stimulation did not affect the direction of coupling, but slightly reduced the amount of data with unidirectional coupling. Rhythms associated with the cardiovascular system (blood pressure variability) were controlled by rhythms of the respiratory system, and the respiratory rhythm controlled the variability of neuronal activity in the reticular formation of the medulla oblongata.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"68 6","pages":"1013 - 1020"},"PeriodicalIF":4.033,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140172428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信