Biophysics最新文献_第5页

Brain Biopotentials as a Factor of Automatic Modulation of Therapeutic Sensory Stimulation Parameters 脑生物电位作为治疗性感觉刺激参数自动调节的一个因素

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925700988

A. I. Fedotchev

引用次数: 0

A Study of the Structure and Function of Musculature in Planarians (Platyhelminthes) 拟涡虫（Platyhelminthes）肌肉组织结构与功能的研究

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S000635092570085X

N. D. Kreshchenko

{"title":"A Study of the Structure and Function of Musculature in Planarians (Platyhelminthes)","authors":"N. D. Kreshchenko","doi":"10.1134/S000635092570085X","DOIUrl":"10.1134/S000635092570085X","url":null,"abstract":"<div><p>The functional activity and morphological characteristics of the musculature of planarians have been studied. A physiological study conducted on isolated muscle cells of the planarian <i>Procerodes littoralis</i> (Turbellaria, Platyhelminthes) showed that caffeine in concentrations of 1–10 mM and depolarization caused by a high content of potassium ions (20–75 mM) had a dose-dependent stimulatory effect on the musculature of planarians. Tapsigargin and cyclopiazonic acid, which are calcium reuptake blockers in the sarcoplasmic reticulum depot, reduced the number of muscle responses induced by both caffeine and depolarization. In addition, contractions of single <i>P. littoralis</i> muscle fibers induced by a medium with a high content of potassium ions were inhibited by ryanodine, which in mM concentrations is a blocker of the ryanodine calcium channels of the sarcoplasmic reticulum. The data we obtained suggest that intracellular calcium depots, as an intracellular source of calcium ions, play a role in the mechanism of contractile muscle response in flatworms. Histochemical examination using frozen sections and staining with fluorescently labeled phalloidin demonstrated the conservative organization of the body wall musculature of <i>Schmidtea mediterranea</i> planarians. The musculature of the body wall contains circular, diagonal, and longitudinal muscle fibers. The thinnest circular fibers are densely packed in the outer layer of the musculature. The longitudinal fibers are the thickest and are mostly assembled in pairs. The diagonal fibers extend in two directions at an angle of 110–115 degrees to each other. Dorso-ventral muscle bundles penetrate the planarian body. The intestinal lumen is surrounded by thin longitudinal muscle fibers. These experiments have shown that planarians can be successfully used as a model object for studying the mechanisms of muscle contraction in flatworms.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 5","pages":"796 - 806"},"PeriodicalIF":4.033,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure and Cooperative Interactions between the Guanine Quadruplexes of the Promoter of Gallus gallus βA-Globin Gene 鸡β a -珠蛋白基因启动子鸟嘌呤四联体的结构及协同相互作用

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925700812

E. V. Marilovtseva, D. O. Koshkina, A. V. Feofanov, V. M. Studitsky

{"title":"Structure and Cooperative Interactions between the Guanine Quadruplexes of the Promoter of Gallus gallus βA-Globin Gene","authors":"E. V. Marilovtseva, D. O. Koshkina, A. V. Feofanov, V. M. Studitsky","doi":"10.1134/S0006350925700812","DOIUrl":"10.1134/S0006350925700812","url":null,"abstract":"<div><p>Guanine quadruplexes are secondary nucleic acid structures present in the genomes of all eukaryotes, from yeast to mammals, where they play an important role in maintaining telomere integrity, creating TAD boundaries, and regulating transcription, alternative splicing, and translation. It was found that, contributing to the formation of a nucleosome-free region, guanine quadruplexes formed by two G-rich motifs inside the replication origin localized in the promoter of the <i>Gallus gallus</i> β<sup>A</sup>-globin gene are necessary for the initiation of replication. In our work, circular dichroism spectroscopy was used to study the structures and dynamic properties of guanine quadruplexes formed by the β<sup>A</sup>-globin promoter/origin sequence in vitro. The data obtained show that quadruplexes located on the same DNA fragment are formed cooperatively, influencing the structures of each other and the entire DNA fragment on which they are located. These data suggest that the structures of guanine quadruplexes may be determined by their genomic environment, and also help explain some of the properties of quadruplexes observed in vivo.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 5","pages":"753 - 761"},"PeriodicalIF":4.033,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein Adsorption on Polymeric Nanoparticles: Impact of Polyvinyl Alcohol 聚乙烯醇对蛋白质在高分子纳米颗粒上吸附的影响

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925700848

O. E. Kamaeva, M. B. Sokol, I. A. Gulyaev, M. A. Klimenko, N. G. Yabbarov, M. R. Mollaeva, M. V. Chirkina, S. A. Brezgin, S. L. Kuznetsov, E. D. Nikolskaya

{"title":"Protein Adsorption on Polymeric Nanoparticles: Impact of Polyvinyl Alcohol","authors":"O. E. Kamaeva, M. B. Sokol, I. A. Gulyaev, M. A. Klimenko, N. G. Yabbarov, M. R. Mollaeva, M. V. Chirkina, S. A. Brezgin, S. L. Kuznetsov, E. D. Nikolskaya","doi":"10.1134/S0006350925700848","DOIUrl":"10.1134/S0006350925700848","url":null,"abstract":"<p>The protein corona has a significant impact on the biodistribution, pharmacokinetics and therapeutic functionality of nanosystems, which determines the need for an in-depth study of the factors influencing its formation. The auxiliary substances included in the nanoparticles, in particular, polyvinyl alcohol, can affect their physico-chemical properties, thereby affecting the formation of a protein corona. In this work, nanoparticles based on a copolymer of lactic and glycolic acids loaded with paclitaxel were synthesized by single emulsification method using various concentrations of polyvinyl alcohol. The obtained nanoparticles were characterized by dynamic and electrophoretic light scattering methods. The adsorption models of Langmuir, Freundlich, Langmuir–Freundlich and Brunauer, Emmett and Teller were used to describe the process of protein adsorption on the surface of nanoparticles. The obtained data indicate that the concentration of adsorbed proteins decreases when the residual polyvinyl alcohol concentration on the particle surface is increased. The revealed relationship will allow optimizing the design of nanosystems based on a copolymer of lactic and glycolic acids in order to increase the effectiveness of therapy.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 5","pages":"787 - 795"},"PeriodicalIF":4.033,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and Dimensional Diversity of Gastric Juice Extracellular Vesicles in the Norm and at Gastric Cancer 正常人和胃癌患者胃液细胞外囊泡形态和尺寸的差异

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925701167

A. A. Beliaeva, V. A. Pogosyan, G. O. Skryabin, S. A. Galetsky, D. V. Bagrov, A. V. Moiseenko, T. S. Trifonova, O. T. Imaraliev, I. A. Karasev, E. M. Tchevkina

{"title":"Morphological and Dimensional Diversity of Gastric Juice Extracellular Vesicles in the Norm and at Gastric Cancer","authors":"A. A. Beliaeva, V. A. Pogosyan, G. O. Skryabin, S. A. Galetsky, D. V. Bagrov, A. V. Moiseenko, T. S. Trifonova, O. T. Imaraliev, I. A. Karasev, E. M. Tchevkina","doi":"10.1134/S0006350925701167","DOIUrl":"10.1134/S0006350925701167","url":null,"abstract":"<p>Complex morphological and dimensional profiling of extracellular vesicles of human gastric juice by cryo-electron microscopy has been performed. It has been shown that in gastric cancer, the proportion of classical individual vesicles surrounded by a single layer of the plasma membrane increases statistically significantly (up to 74% versus 40% in healthy controls), while the representation of multilayer forms decreases. A comparative analysis of data from cryo-electron microscopy and analysis of nanoparticle trajectories has been carried out, demonstrating that cryo-electron microscopy reveals a significantly higher proportion of particles in the range of 40–70 nm. Thus, the paper describes for the first time the composition of extracellular vesicles of gastric juice and reveals significant differences in the morphological distribution between the norm and pathology, as well as systematic discrepancies between the methods of cryo-electron microscopy and analysis of nanoparticle trajectories in the direction of greater sensitivity of cryo-electron microscopy to small vesicles.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 6","pages":"1073 - 1081"},"PeriodicalIF":4.033,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Deep Learning Approach to Predict Histone Variant Effects on Nucleosome Stability 预测组蛋白变异对核小体稳定性影响的深度学习方法

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925701027

E. A. Bogdanova, A. V. Chernukhin, A. O. Matyushevskaya, V. N. Novoseletsky, G. A. Komarova, A. K. Shaytan

{"title":"A Deep Learning Approach to Predict Histone Variant Effects on Nucleosome Stability","authors":"E. A. Bogdanova, A. V. Chernukhin, A. O. Matyushevskaya, V. N. Novoseletsky, G. A. Komarova, A. K. Shaytan","doi":"10.1134/S0006350925701027","DOIUrl":"10.1134/S0006350925701027","url":null,"abstract":"<p>Histones play a key role in chromatin organization and gene regulation by modulating nucleosome stability and DNA accessibility. Different histone variants can alter the packing density of DNA and affect cell function. This study demonstrates the successful application of the developed ProBAN neural network to predict the affinity of protein–protein interactions, which can be used to assess the effect of various histone variants on the stability of nucleosomes. It has been shown that the incorporation of histone variants into the nucleosome can stabilize or destabilize the structure of the histone octamer through altered patterns of intermolecular interactions. In particular, the replacement histone variants H2A.Z, TSH2A.1-TSH2B.1 (in the form of a dimer), and H3.3 form more stable structures inside the nucleosome than canonical histones, while other variants such as TSH2A.1, TSH2B.1 (separately) and H3.6, demonstrated less stable interactions. The observed differences may explain how cells regulate genes in specialized tissues or during development. These results demonstrate the effect of histone sequence variations on the stability of nucleosomes, which is important for the regulation of transcription and epigenetic mechanisms. The ProBAN algorithm has demonstrated high prediction accuracy as a valuable tool for further study of the effect of histone variants on chromatin functioning and disease development.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 6","pages":"950 - 959"},"PeriodicalIF":4.033,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidized Disulfiram Derivatives Activate an Integrated Stress Response and Cause Paraptosis of Human Cancer Cells BT474 氧化双硫仑衍生物激活综合应激反应并引起人类癌细胞BT474的凋亡

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925700964

M. E. Solovieva, I. V. Odinokova, Yu. V. Shatalin, A. A. Mishukov, E. L. Holmuhamedov, V. S. Akatov

引用次数: 0

Piezopulsometric Analysis of the Cardiovascular System Functional State in Patients with Metabolic Syndrome 代谢综合征患者心血管系统功能状态的压脉冲分析

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925700976

M. F. Ballyuzek, A. I. Burdygin, E. E. Guryanova, K. B. Ivanov, K. V. Sobol, I. V. Shemarova, V. P. Nesterov

{"title":"Piezopulsometric Analysis of the Cardiovascular System Functional State in Patients with Metabolic Syndrome","authors":"M. F. Ballyuzek, A. I. Burdygin, E. E. Guryanova, K. B. Ivanov, K. V. Sobol, I. V. Shemarova, V. P. Nesterov","doi":"10.1134/S0006350925700976","DOIUrl":"10.1134/S0006350925700976","url":null,"abstract":"<div><p>The results of using differential heart rate monitoring for noninvasive screening and analysis of the functional state of the cardiovascular system and the nature of its autonomous regulation in patients with metabolic syndrome are presented. The indicators characterizing the general cardiohemodynamics, as well as the variability of the <i>V</i><sub>maxPP</sub> and PP parameters of cyclic waves of blood pulse pressure, reflecting the regulatory effect of peripheral mechanisms of the autonomic nervous system and the endocrine system on the cardiovascular system of patients with metabolic syndrome, are evaluated. The involvement and role of myocytes of the wall of the left ventricle of the heart as a universal muscle effector providing both the main pumping function of the cardiovascular system and the possibility of regulating the strength and speed of contractions of the left ventricle of the heart is considered. The prognostic significance of a comprehensive piezopulsometric examination of the cardiovascular system of patients for early noninvasive detection of signs of metabolic syndrome and its treatment is discussed.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 5","pages":"904 - 913"},"PeriodicalIF":4.033,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the Possibility of Using the Registration of Fluorescence of Stained Cells Isolated from the Skin to Study the Severity of Oxidative Stress 利用皮肤分离的染色细胞的荧光登记来研究氧化应激严重程度的可能性评价

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925700952

L. A. Romodin, A. A. Moskovskij, E. S. Chelarskaya, C. C. Sodboev, O. V. Nikitenko, T. M. Bychkova, A. V. Mitrofanova, V. I. Pustovoit

{"title":"Evaluation of the Possibility of Using the Registration of Fluorescence of Stained Cells Isolated from the Skin to Study the Severity of Oxidative Stress","authors":"L. A. Romodin, A. A. Moskovskij, E. S. Chelarskaya, C. C. Sodboev, O. V. Nikitenko, T. M. Bychkova, A. V. Mitrofanova, V. I. Pustovoit","doi":"10.1134/S0006350925700952","DOIUrl":"10.1134/S0006350925700952","url":null,"abstract":"<div><p>Oxidative stress is a key link in the development of many pathological processes. Fluorescent dyes sensitive to reactive oxygen species, in particular dichlorofluorescein and its derivatives, are widely used for its evaluation. These dyes are actively used when working with cell cultures, but there is very little data on their use for the analysis of cells isolated directly from animal tissues. The present study was devoted to the investigation of the possibility of using 6-carboxy-dichlorofluorescein to register oxidative stress in cells isolated from rat skin flaps. To induce reactive oxygen species, skin fragments were incubated in a medium with hydrogen peroxide, after which cell suspensions were prepared from them. The cells were stained with 6-carboxy-dichlorofluorescein and Hoechst-33342, which allowed the fluorescent signal to be normalized by the number of cells in the sample. In separate experiments, the precursor of 6-carboxy-dichlorofluorescein was injected intermediately to live animals. Evaluation of the fluorescence ratio of 6-carboxy-2',7'-dichlorofluorescein and Hoechst-33342 showed the possibility of registering only pronounced oxidative stress, while it was not possible to distinguish its degree or record low-intensity changes. Additionally, microscopic analysis revealed the presence of cellular aggregates and fragments of the intercellular matrix, which reduced the reliability of the results. The data we obtained indicate the limitations of the method of staining skin cells with dichlorofluorescein derivatives to assess the intensity of oxidative stress in native tissue.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 5","pages":"886 - 893"},"PeriodicalIF":4.033,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Combined Static and Alternating Low-Frequency Magnetic Fields on the Respiratory Burst Intensity in Mouse Neutrophils Depending on the Magnitude of the Alternating Field Component 静交变低频磁场对小鼠中性粒细胞呼吸爆发强度随交变磁场分量大小的影响

IF 4.033

Biophysics Pub Date : 2026-04-01 DOI: 10.1134/S0006350925701064

V. V. Novikov, E. V. Yablokova

引用次数: 0