Biophysics最新文献_第4页

Ambiol Modulates the Functional Characteristics of Pea Seedlings Mitochondria 安倍醇调节豌豆幼苗线粒体的功能特征

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700295

I. V. Zhigacheva, N. I. Krikunova, Yu. V. Kuznetsov, A. N. Goloshchapov

{"title":"Ambiol Modulates the Functional Characteristics of Pea Seedlings Mitochondria","authors":"I. V. Zhigacheva, N. I. Krikunova, Yu. V. Kuznetsov, A. N. Goloshchapov","doi":"10.1134/S0006350924700295","DOIUrl":"10.1134/S0006350924700295","url":null,"abstract":"<div><p>The effect of the plant growth regulator ambiol on the functional state of mitochondria of etiolated pea seedlings was studied. The drug prevented the activation of lipid peroxidation in mitochondrial membranes in the concentration range of 10<sup>–5</sup>–10<sup>–6</sup> and 10<sup>–9</sup> M. The introduction of ambiol into the incubation medium of these organelles resulted in an increase in the oxidation rates of NAD-dependent substrates. The most effective concentrations were 10<sup>–5</sup> and 10<sup>–6</sup> M. Changes in the bioenergetic characteristics of mitochondria were accompanied by changes in the fatty acid composition of mitochondrial membranes. The most significant changes occurred in the content of 18:2ω6 and 18:3ω3 fatty acids. It was hypothesized that an increase in the content of these fatty acids in the membranes of mitochondria indicated an increase in the content of cardiolipin, which ensured the effective functioning of the mitochondrial respiratory chain and, consequently, the maintenance of energy metabolism at a high level. The treatment of pea seeds with ambiol not only prevented a decrease in the growth rate of pea seedlings under conditions of water stress, but also stimulated their growth.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"231 - 236"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Combined Effects of Electrochemical and Ultrasonic Treatment for Water Purification from Pathogenic Contaminants Based on the Example of Escherichia coli 以大肠埃希氏菌为例，探讨电化学和超声波处理对净化水中致病污染物的联合作用

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700325

S. B. Bibikov, A. I. Sergeev, I. I. Barashkova, M. V. Motyakin

引用次数: 0

The Relationship between the Composition of Natural Water and State Lipid Peroxidation in Biological Objects 天然水成分与生物体内脂质过氧化状态之间的关系

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700350

L. N. Shishkina, A. S. Dubovik, V. O. Shvydkiy, M. V. Kozlov, E. V. Shtamm, A. G. Georgiadi

{"title":"The Relationship between the Composition of Natural Water and State Lipid Peroxidation in Biological Objects","authors":"L. N. Shishkina, A. S. Dubovik, V. O. Shvydkiy, M. V. Kozlov, E. V. Shtamm, A. G. Georgiadi","doi":"10.1134/S0006350924700350","DOIUrl":"10.1134/S0006350924700350","url":null,"abstract":"<div><p>The relationship between the compositions of five natural water samples and the parameters of the lipid peroxidation regulation in a model system based on natural phospholipids, as well as their participation in the toxicity of natural water, have been studied. It has been shown that the presence of N- and P-containing compounds in natural water samples led to inhibition of lecithin autooxidation and luminescence intensity of luminous bacteria; it had a significant effect on spontaneous aggregation of lecithin and an increase in the content of hydrophobic compounds increased the negative value of the ζ potential of its particles. The high sensitivity of the parameters of the lipid peroxidation regulation system to the composition of natural water, even at low concentrations of its components, allows them to be considered as promising indicators for assessing the effect of natural water on biological objects. Mathematical processing of ultraviolet spectra of natural water samples using the Gauss method can be used as an express test for the analysis of its hydrochemical composition. The effect of natural water components on the state of membranes and intracellular processes of biological objects has been confirmed by biotesting methods.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"292 - 298"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An X-Ray Diffraction Study of Lipid Films with ICHPHAN 用 ICHPHAN 对脂质薄膜进行 X 射线衍射研究

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700258

A. V. Krivandin, A. N. Goloshchapov

引用次数: 0

Gold and Silver Compounds as Potential Antitumor Drugs 作为潜在抗肿瘤药物的金银化合物

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700416

L. A. Ostrovskaya, D. B. Korman, E. I. Nekrasova, A. K. Chigasova, N. V. Bluhterova, V. A. Rikova, M. M. Fomina, Yu. A. Khochenkova, K. A. Abzaeva

引用次数: 0

Reductive Nitrosylation of Hemoglobin and Myoglobin and its Antioxidant Effect 血红蛋白和肌红蛋白的还原亚硝基化及其抗氧化作用

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700210

K. B. Shumaev, D. I. Grachev, O. V. Kosmachevskaya, A. F. Topunov, E. K. Ruuge

{"title":"Reductive Nitrosylation of Hemoglobin and Myoglobin and its Antioxidant Effect","authors":"K. B. Shumaev, D. I. Grachev, O. V. Kosmachevskaya, A. F. Topunov, E. K. Ruuge","doi":"10.1134/S0006350924700210","DOIUrl":"10.1134/S0006350924700210","url":null,"abstract":"<div><p>Angeli’s salt is a nitroxyl donor, due to which it can prevent the negative consequences of hemolysis, such as the vasoconstrictive effect of free hemoglobin in blood plasma. However, the molecular mechanisms of the interaction of nitroxyl with various hemoproteins have not been sufficiently elucidated. It is known that oxoferryl forms of hemoproteins arising from oxidative stress are strong prooxidants. In this study, the reductive nitrosylation of meth- and oxoferryl forms of hemoglobin and myoglobin with nitroxyl was investigated. The experiments were carried out in vitro by detecting nitrosyl forms of hemoproteins using electron paramagnetic resonance spectroscopy. The results indicate the antioxidant effect of Angeli’s salt in systems simulating the oxidation of hemoglobin or myoglobin by hydrogen peroxide. As well, the addition of hydrogen peroxide to hemoglobin and myoglobin metforms led to the appearance of an EPR signal of free radicals with <i>g</i> = 2.005 associated with the protein part of these hemoproteins. Thus, nitroxyl acted simultaneously as a reducing and nitrosylating agent, thereby preventing the formation of oxoferryl forms of hemoproteins. The therapeutic properties of Angeli’s salt may be largely related to the antioxidant effect exerted on blood components.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"195 - 200"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Study of the Physiological Role of the Spectral Composition of Daily Light Exposure in the Development of Childhood Myopia 关于日常光照的光谱成分在儿童近视发展中的生理作用的研究

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700398

N. N. Trofimova, Y. S. Petronyuk, E. A. Khramtsova, V. V. Sokolova, K. G. Antipova, T. S. Gurieva, E. I. Mednikova

{"title":"A Study of the Physiological Role of the Spectral Composition of Daily Light Exposure in the Development of Childhood Myopia","authors":"N. N. Trofimova, Y. S. Petronyuk, E. A. Khramtsova, V. V. Sokolova, K. G. Antipova, T. S. Gurieva, E. I. Mednikova","doi":"10.1134/S0006350924700398","DOIUrl":"10.1134/S0006350924700398","url":null,"abstract":"<div><p>The present study is an attempt to experimentally create conditions for the occurrence of childhood myopia in laboratory animals. Measurements were performed using ultrasound technique for different age groups of Japanese quail <i>Coturnix japonica dom.</i>, from chicks (10, 25, and 45 days) and up to fully mature birds of the (65 days) in situ. To simulate the processes of myopization, everyday lighting sources with narrow spectrum filters, blue (450 ± 10 nm), red (630 ± 10 nm), and the most eye-friendly yellow (550 ± 20 nm), were used. High-frequency focused ultrasound (30–80 MHz) and the <i>B</i>/<i>Z</i> scanning mode provided high-quality visualization of the fine structure of the chick eye and the accuracy of measuring geometric parameters in its sagittal plane. The obtained data on the size of the eyeball, the thickness of the lens and sclera, cornea, vitreous body, and chorioretinal complex made it possible to trace the age-related changes in the eye during the ontogenesis of the animal. Preliminary data on the elasticity of the sclera of the quail eye were obtained.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"320 - 324"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computer Simulation of Short DNA Fragments Induced by High-LET Charged Particles 高放电带电粒子诱导短 DNA 片段的计算机模拟

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700222

Y. A. Eidelman, I. V. Salnikov, S. G. Andreev

引用次数: 0

The Inhibitory Effect of Oxibiol on the Process of Protein Modification by Water-Soluble Products of Photo-Oxidative Destruction of Bisretinoid A2E 羰基二醇对双维甲酸 A2E 光氧化破坏的水溶性产物修饰蛋白质过程的抑制作用

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700246

A. E. Dontsov, N. L. Aronshtam, M. A. Ostrovsky

{"title":"The Inhibitory Effect of Oxibiol on the Process of Protein Modification by Water-Soluble Products of Photo-Oxidative Destruction of Bisretinoid A2E","authors":"A. E. Dontsov, N. L. Aronshtam, M. A. Ostrovsky","doi":"10.1134/S0006350924700246","DOIUrl":"10.1134/S0006350924700246","url":null,"abstract":"<div><p>We have previously shown that the heteroaromatic antioxidant oxibiol (N-acetylcysteinate 6-hydroxy-2-aminobenzothiazole) inhibited the process of fructosylation of serum albumin. The purpose of this study was to elucidate the inhibitory effect of oxibiol on protein modification by photodestruction products of bisretinoid A2E, the main fluorophore of lipofuscin granules of retinal pigment epithelium cells of the human eye. It was found that the water-soluble fraction obtained from A2E-liposomes irradiated with visible light, unlike the water-soluble fraction from nonirradiated A2E-liposomes, significantly modified albumin after a day of incubation at 37°C. Oxibiol at millimolar concentrations effectively inhibited this process. The inhibitory effect of oxibiol was probably based on its antioxidant activity and the ability to competitively interact with reactive aldehydes formed during the photo-oxidative destruction of A2E-cardiolipin liposomes. The acute toxicity of oxibiol in mice with intraperitoneal administration was studied and the values of <i>LD</i><sub>10</sub> and <i>LD</i><sub>50</sub> were determined. The results indicated the promising use of oxibiol in pharmacology for the prevention and treatment of diseases associated with the development of oxidative stress in various fields of medicine, primarily in ophthalmology.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"219 - 224"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Antimicrobial Activity of Metabolite Complexes of Lactobacilli against Pseudomonas aeruginosa 乳酸菌代谢物复合物对铜绿假单胞菌的抗菌活性

IF 4.033

Biophysics Pub Date : 2024-09-27 DOI: 10.1134/S0006350924700337

S. A. Kishilova, A. Y. Kolokolova, I. V. Rozhkova

{"title":"The Antimicrobial Activity of Metabolite Complexes of Lactobacilli against Pseudomonas aeruginosa","authors":"S. A. Kishilova, A. Y. Kolokolova, I. V. Rozhkova","doi":"10.1134/S0006350924700337","DOIUrl":"10.1134/S0006350924700337","url":null,"abstract":"<div><p>The aim of this study was a comparative assessment of the antagonistic activity of the culture collection strains of <i>Lactobacillus helveticus</i> NK1, <i>Lacticaseibacillus rhamnosus</i> F, and fungal kefir starter culture, as well as their metabolic complexes against representatives of <i>Pseudomonas aeruginosa</i> strains. As a result of the study, the high antagonistic activity of the culture collection lactobacilli, fungal kefir starter culture, and their metabolic complexes against conditionally pathogenic bacteria <i>P. aeruginosa</i>, both for the culture collection type strain and wild-type isolates, was confirmed. The <i>L. helveticus</i> NK1 strain was a more active antagonist of <i>P. aeruginosa</i> compared to <i>L. rhamnosus</i> F, which might be due to its ability to produce peptides with antimicrobial activity. These data confirmed the prospects of using the <i>L. helveticus</i> NK1 strain and fungal kefir starter culture for the development of a fermented milk product enriched with metabolites of probiotic cultures. The results justify further studies to determine the mechanisms of their antimicrobial action.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 2","pages":"278 - 284"},"PeriodicalIF":4.033,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0