Biometals最新文献_第3页

The importance of zinc, copper and manganese and their impact on growth, immunity and fertility of male cattle: a review. 锌、铜、锰的重要性及其对雄性牛生长、免疫和生育力的影响

IF 4.1 3区生物学

Biometals Pub Date : 2025-06-01 Epub Date: 2025-05-19 DOI: 10.1007/s10534-025-00692-8

Prasanth M Nair, Rashika Srivastava, Veena Mani, S Arulkumar, Nitin Tyagi, Goutam Mondal

{"title":"The importance of zinc, copper and manganese and their impact on growth, immunity and fertility of male cattle: a review.","authors":"Prasanth M Nair, Rashika Srivastava, Veena Mani, S Arulkumar, Nitin Tyagi, Goutam Mondal","doi":"10.1007/s10534-025-00692-8","DOIUrl":"10.1007/s10534-025-00692-8","url":null,"abstract":"Trace minerals are essential for the growth, development and total well-being of all living organisms. Although there are various standards regarding the amount of supplementation of minerals, compared with other nutrients like energy, protein, and others; trace minerals are still unexplored, especially in the case of male livestock. These minerals help in improving the growth rate of calves, the utilization of absorbed nutrients, and the immunity of animals thereby preventing metabolic diseases. They also act as growth promoters that can hasten the growth rate of calves and help them reach early puberty. Therefore, the deficiency of these minerals can compromise the growth and development of male calves and impair the fertility of adult bulls. Their physiological importance and role are often underestimated, and their presence in feed in adequate quantities is taken for granted. However, they are essential for maintaining various functions in the body, such as optimising growth, development and reproduction, and fortifying the immune response therefore determining the overall well-being and health status of the animal. The current review highlights specific trace minerals, which include Zn, Cu, and Mn, and their effect on the growth, immunity and reproductive development of males. Artificial insemination is becoming popular in cattle, and this has significantly increased conception rates and per capita milk production. However, due to inadequate management techniques and inadequate feed and trace mineral intakes, India has a very low (30%) AI coverage. Male breeding animals have different needs from lactating and maintenance females. They influence the productivity of a large herd of animals. As the guidelines for trace mineral requirements for breeding animals are not specifically provided, the needs of male livestock are often misinterpreted.","PeriodicalId":491,"journal":{"name":"Biometals","volume":" ","pages":"763-784"},"PeriodicalIF":4.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis, characterization and biological potential of nitroso-R salt with transition metal complexes. 含过渡金属配合物的亚硝基r盐的合成、表征及生物潜力。

IF 4.1 3区生物学

Biometals Pub Date : 2025-05-29 DOI: 10.1007/s10534-025-00698-2

Saddam Hussain, Mehreen Zafar, Nauman Ali, Takashiro Akitsu, Waseem Hassan

{"title":"Synthesis, characterization and biological potential of nitroso-R salt with transition metal complexes.","authors":"Saddam Hussain, Mehreen Zafar, Nauman Ali, Takashiro Akitsu, Waseem Hassan","doi":"10.1007/s10534-025-00698-2","DOIUrl":"https://doi.org/10.1007/s10534-025-00698-2","url":null,"abstract":"Coordination complexes exhibited interesting potentials in different fields such as medical, industrial, pharmaceutical, and analytical. They possess versatile biological applications in drugs synthesis, extraction of noble metals like silver and gold from their ores and in metals purification. In current project we aim to synthesized and characterized transition metal complexes like Mn, Fe, Co, Ni, Cu and Zn using Nitroso-R Salt as a ligand. This study presents a novel approach to develop stable metal complexes using Nitroso R Salt, a ligand comprising nitrosyl, hydroxyl, and sulfonate groups. This study offers a combined analysis of both chemical and functional properties, which was not commonly investigated with NRS, by combining biological screening with thorough structural characterizations (FTIR, CHN, XRD and UV). The complexes were then subjected to various tests to explore their deoxyribose degradation inhibition potential, antioxidant and antimicrobial potential. Copper and Zinc Complexes showed good potential 173 and 182% against iron at 100 mM. The highest potential was recorded for Nickel complex potential 2, 7 and 14% against H2O2, Fe (II) and in combine form. It was further confirmed respectively by antioxidant DPPH assay. Ligand and complexes showed good inhibitory potential by showing 18.66, 39.32, 38.22, 28.89, 20.71, 19.14 and 28.89% for NRS, Iron, Cobalt, Nickel, Zinc, Copper and Manganese complexes at high concentration of 200uM, respectively. The complexes showed promising antibacterial and antioxidant properties. Nickel complexes demonstrated a strong inhibition against reactive species, but copper and zinc complexes showed a notably high antioxidant capacity. Effective inhibitory zones were found in antimicrobial testing, especially for Fe, Mn, Co, and Cu complexes against Klebsiella pneumoniae and Bacillus subtilis. However, further studies are needed to examine the exact mechanisms of action of these discoveries, which point to possible biomedical uses.","PeriodicalId":491,"journal":{"name":"Biometals","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermophiles in nanosized biocalcification: a novel approach for heavy metal remediation. 纳米级生物钙化中的嗜热菌：重金属修复的新方法。

IF 4.1 3区生物学

Biometals Pub Date : 2025-05-24 DOI: 10.1007/s10534-025-00700-x

Sujata Negi, Shagun Sharma, Deepak Pant, Sonali Sharma, Kalpana Chauhan, Anand Giri, Manoj Kumar, Kulamani Parida

{"title":"Thermophiles in nanosized biocalcification: a novel approach for heavy metal remediation.","authors":"Sujata Negi, Shagun Sharma, Deepak Pant, Sonali Sharma, Kalpana Chauhan, Anand Giri, Manoj Kumar, Kulamani Parida","doi":"10.1007/s10534-025-00700-x","DOIUrl":"https://doi.org/10.1007/s10534-025-00700-x","url":null,"abstract":"Bio deposition of minerals is a prevalent occurrence in the biological realm, facilitated by various organisms such as bacteria, fungi, protists, and plants. Calcium carbonate is one such mineral that precipitates naturally as a consequence of microbial metabolic processes. This study investigates an innovative approach for MICP- mediated heavy metal remediation, carbon dioxide (CO2) sequestration by utilizing thermophilic microorganisms isolated from such geographical area which is not yet been subjected to any systematic scientific study. Beyond the well-established urea hydrolysis pathway, this research highlights the contribution of non-ureolytic MICP mechanisms driven by the oxidation of organic compounds within the bacterial extracellular polymeric substances and cell wall components of Bacillus licheniformis. Notably, both strains of Bacillus licheniformis redirect its great potential towards biocalcification yielding 89.36 ± 1.8, 88.21 ± 1.5 mg CaCO3 cells/ml and 90% efficiency for heavy metal remediation with the formation of nanosized (35.85 nm, 38.58 nm) biominerals. The influence of various parameters, such as temperature, pH, incubation time, CO2 concentration, and calcium concentration on maximum CaCO3 biosynthesis was evaluated. FTIR, XRD, and SEM-EDX analyses confirmed characteristic peaks for both calcite and vaterite polymorphs, consistent with these Pb incorporation into the mineral structure, rather than surface adsorption is observed. These comparative findings provide valuable insights for promising bioremediation approach for the sustainable, eco-friendly, energy-efficient immobilization of metal contaminants and bio-based carbonate production for efficient CO2 sequestration.","PeriodicalId":491,"journal":{"name":"Biometals","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic applications of quercetin-metallic complexes: a review. 槲皮素-金属配合物的治疗应用综述。

IF 4.1 3区生物学

Biometals Pub Date : 2025-05-22 DOI: 10.1007/s10534-025-00696-4

Nasreen Ramzan, Huma Butt, Muhammad Azeem, Muhammad Hanif, Khalid Mahmood, Sadia Rehman, Dure Shahwar, Muhammad Zeeshan, Qurat-Ul-Ain Ahmad, Mehreen Jabeen

{"title":"Therapeutic applications of quercetin-metallic complexes: a review.","authors":"Nasreen Ramzan, Huma Butt, Muhammad Azeem, Muhammad Hanif, Khalid Mahmood, Sadia Rehman, Dure Shahwar, Muhammad Zeeshan, Qurat-Ul-Ain Ahmad, Mehreen Jabeen","doi":"10.1007/s10534-025-00696-4","DOIUrl":"https://doi.org/10.1007/s10534-025-00696-4","url":null,"abstract":"Flavonoids, especially quercetin, are widely recognized for their antioxidant, anti-inflammatory, anti-microbial, anti-diabetic, and anti-tumor properties, but their clinical application is limited by poor permeability. Different techniques of permeability enhancement of quercetin, i.e., preparation of phytosomes, conjugation with glucose, and interaction of piperine, are reported extensively, but formation of metal complexes is considered more precise and reliable. This review focuses on research conducted on the chelation of quercetin with metals, i.e., copper (II), zinc (II), iron and ruthenium. In-vitro and in-vivo therapeutic activities, biomedical applications of metal/quercetin inclusion complexes and discussions of stability concerns of quercetin alone and its metallic complexes. Conjugation of metals with quercetin augments its potential to the next level. Numerous studies revealed that quercetin/copper (Q/Cu) and quercetin/zinc (Q/Zn) can become efficacious candidates in acute myeloid leukaemia, orthopaedics and severe acute respiratory syndrome coronavirus (SARS-CoV) infection.","PeriodicalId":491,"journal":{"name":"Biometals","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of copper-induced oxidative damage on the endoplasmic reticulum quality control system in broiler kidneys. 铜诱导的氧化损伤对肉鸡肾脏内质网质量控制系统的影响。

IF 4.1 3区生物学

Biometals Pub Date : 2025-05-22 DOI: 10.1007/s10534-025-00695-5

Feiyang Ma, Mengran Wang, Gaolong Zhong, Jianzhao Liao, Yihui Huo, Zekai Wang, Shaojun He

{"title":"The impact of copper-induced oxidative damage on the endoplasmic reticulum quality control system in broiler kidneys.","authors":"Feiyang Ma, Mengran Wang, Gaolong Zhong, Jianzhao Liao, Yihui Huo, Zekai Wang, Shaojun He","doi":"10.1007/s10534-025-00695-5","DOIUrl":"https://doi.org/10.1007/s10534-025-00695-5","url":null,"abstract":"Copper (Cu) is a pervasive element utilized in economic animal production. However, overuse can have toxic effects on animals and threaten public food safety. To gain a deeper understanding of the mechanisms underlying Cu-induced nephrotoxicity, an in-depth analysis was conducted on the effects of Cu on the renal endoplasmic reticulum quality control (ERQC) system. In the course of this experiment, one-day-old chicks were fed diets comprising Cu levels (11, 110, 220 and 330 mg/kg) for 49 days. Our findings indicate that an excess of Cu may result in oxidative stress, which may then induce tissue damage within the kidney. Furthermore, the experimental results indicated that elevated Cu levels may disrupt to the ERQC system in chicken kidneys. The mRNA levels of GRP78, GRP94, ATF4, IRE1, and XBP1, as well as the protein levels of GRP78, GRP94, IRE1, XBP1, and CHOP, were markedly elevated in all treatment groups relative to the control group. Conversely, the mRNA and protein levels of eIF2α and ATF6 exhibited a notable decline with the increase in Cu levels. Similarly, RTN3, ATL1, and ATL2 mRNA levels as well as RTN3 and ATL3 protein levels exhibited a notable elevation in conjunction with an appreciable decline in FAM134B and SEC62 mRNA and protein levels, respectively, as Cu levels increased. Furthermore, bioinformatics analyses indicated a correlation between oxidative damage and ERQC markers. The above results suggest that Cu-induced oxidative damage may injure to chicken kidneys via disturbances in the ERQC system.","PeriodicalId":491,"journal":{"name":"Biometals","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ferrous sulfate and lipopolysaccharide co-exposure induce neuroinflammation, neurobehavioral motor deficits, neurodegenerative and histopathological biomarkers relevant to Parkinson's disease-like symptoms in Wistar rats. 硫酸亚铁和脂多糖共暴露诱导Wistar大鼠神经炎症、神经行为运动缺陷、神经退行性和与帕金森病样症状相关的组织病理学生物标志物。

IF 4.1 3区生物学

Biometals Pub Date : 2025-05-16 DOI: 10.1007/s10534-025-00693-7

Shivam Kumar Pandey, Anjuman Nanda, Avtar Singh Gautam, Apurva Chittoda, Aman Tiwari, Rakesh Kumar Singh

{"title":"Ferrous sulfate and lipopolysaccharide co-exposure induce neuroinflammation, neurobehavioral motor deficits, neurodegenerative and histopathological biomarkers relevant to Parkinson's disease-like symptoms in Wistar rats.","authors":"Shivam Kumar Pandey, Anjuman Nanda, Avtar Singh Gautam, Apurva Chittoda, Aman Tiwari, Rakesh Kumar Singh","doi":"10.1007/s10534-025-00693-7","DOIUrl":"https://doi.org/10.1007/s10534-025-00693-7","url":null,"abstract":"Parkinson's disease (PD) is a progressive neurodegenerative disorder characterized by the loss of dopaminergic neurons in the substantia nigra region of the brain. Although iron is one of the essential micronutrients in the brain, its excess exposure and accumulation in the brain substantia nigra and striatum regions may induce critical pathological changes relevant to PD. This study has evaluated neurobehavioral, biochemical, and structural alterations resembling PD-like symptoms induced through a 4-week co-exposure of ferrous sulfate (FeSO4) with lipopolysaccharide (LPS) in Wistar rats. Our results revealed motor deficits, oxidative stress, neuroinflammation, iron dysregulation, protein aggregation, ferroptosis, and apoptotic cell death. Notably, we observed decreased tyrosine hydroxylase levels and increased α-synuclein accumulation, consistent with PD pathology. The immunohistopathological assessments showed astrocyte activation and iron deposition, supporting their roles in neuroinflammation and oxidative stress. Furthermore, we identified alterations in apoptosis and ferroptosis markers, suggesting dose-related involvement of FeSO4 in neuronal death in the rat brain. These findings have highlighted the multifaceted mechanisms during the co-exposure of FeSO4 and LPS-induced neurodegeneration and neuroinflammation relevant to PD. This study emphasizes that therapeutic targeting of these pathological mechanisms may offer a promising therapeutic intervention in PD.","PeriodicalId":491,"journal":{"name":"Biometals","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A dual-modal approach to lung cancer treatment: in vitro and in silico. Evaluation of a hybrid nanocomposite for synergistic chemotherapy. 肺癌治疗的双模态方法：体外和计算机。混合纳米复合材料协同化疗的评价。

IF 4.1 3区生物学

Biometals Pub Date : 2025-05-14 DOI: 10.1007/s10534-025-00694-6

Omar Alnasra, Fawwaz I Khalili, Lama Hamadneh, Mohammad Alwahsh, Rana Omar, Amani AlDoridee, Aya Hasan

{"title":"A dual-modal approach to lung cancer treatment: in vitro and in silico. Evaluation of a hybrid nanocomposite for synergistic chemotherapy.","authors":"Omar Alnasra, Fawwaz I Khalili, Lama Hamadneh, Mohammad Alwahsh, Rana Omar, Amani AlDoridee, Aya Hasan","doi":"10.1007/s10534-025-00694-6","DOIUrl":"https://doi.org/10.1007/s10534-025-00694-6","url":null,"abstract":"This study investigates the therapeutic potential of a nanosilica-cysteine composite loaded with arsenic trioxide (SC-As) in combination with cisplatin (CIS), paclitaxel (PTX), and doxorubicin (DOX) for lung/breast cancer treatment. Through comprehensive synthesis, characterization (ATR-FTIR, XRD, SEM, TEM, DLS), and cytotoxicity assessments, SC-As demonstrated superior potency with IC₅₀ values as low as 7.29 ± 1.40 µM in lung cancer (A549) and 8.60 ± 1.20 µM in breast cancer (MCF-7) cell lines. This study employs a dual-modal approach, combining in silico computational predictions (CompuSyn) with in vitro experiments to evaluate synergistic chemotherapy regimens, ensuring robust validation of therapeutic outcomes. The computational synergy analysis and the experimental validation in lung cancer cell lines revealed synergistic interactions between SC-As and CIS (CI < 1), enabling significant dose reductions (DRI > 1). Conversely, antagonism was observed with PTX and DOX in A549 cells, though H1299 cells exhibited unanticipated synergistic interactions with PTX/DOX. Given that H1299 cells represent a more aggressive and metastatic form of lung cancer, these results suggest that PTX and DOX combinations may have enhanced therapeutic potential in treating highly malignant lung cancer subtypes. These findings underscore the composite's potential as a targeted delivery system and highlight the necessity of integrating computational predictions with empirical validation to optimize combinatorial efficacy and minimize toxicity, providing a foundation for future in vivo and clinical studies.","PeriodicalId":491,"journal":{"name":"Biometals","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anticancer potential of lactoferrin: effects, drug synergy and molecular interactions 乳铁蛋白的抗癌潜力：作用、药物协同作用和分子相互作用。

IF 4.1 3区生物学

Biometals Pub Date : 2025-03-21 DOI: 10.1007/s10534-025-00672-y

D. B. León-Flores, L. I. Siañez-Estada, B. F. Iglesias-Figueroa, T. S. Siqueiros-Cendón, E. A. Espinoza-Sánchez, A. Varela-Ramírez, R. J. Aguilera, Q. Rascón-Cruz

{"title":"Anticancer potential of lactoferrin: effects, drug synergy and molecular interactions","authors":"D. B. León-Flores, L. I. Siañez-Estada, B. F. Iglesias-Figueroa, T. S. Siqueiros-Cendón, E. A. Espinoza-Sánchez, A. Varela-Ramírez, R. J. Aguilera, Q. Rascón-Cruz","doi":"10.1007/s10534-025-00672-y","DOIUrl":"10.1007/s10534-025-00672-y","url":null,"abstract":"<div>Cancer treatment is among today’s most active and challenging research fields. In recent years, significant progress has been made in developing new cancer therapies, including nutraceuticals and natural compounds with anticancer properties. Lactoferrin, a glycoprotein present in mammals, is of significant interest due to its pleiotropic behavior, demonstrating a broad spectrum of biological activities such as antimicrobial, antioxidant, anti-inflammatory, immunomodulatory, and anticancer effects. In this review, we examine the current knowledge of Lf's role in cancer. In addition, it exhibits a synergistic effect along with conventional drugs, potentially enhancing their efficacy and, at the same time, reducing the side effects associated with most traditional therapies. However, it is essential to consider the precise molecular mechanism by which Lf exerts its antitumor activity. Searching interactions with several molecules can provide insight into this mechanism. Additionally, finding lactoferrin receptors can improve the strategies for the specific release of the conjugates. For all these reasons, Lactoferrin becomes a potential therapeutic agent that should be examined in depth.</div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":"38 2","pages":"465 - 484"},"PeriodicalIF":4.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustained release of Sr and Ca from a micronanotopographic titanium surface improves osteoblast function 从微纳钛表面持续释放硒和钙可改善成骨细胞功能。

IF 4.1 3区生物学

Biometals Pub Date : 2025-03-17 DOI: 10.1007/s10534-025-00668-8

Luan Viana Faria, Maria Eduarda Scordamaia Lopes, Diego Pedreira de Oliveira, Fernando Santos da Silva, Cecilio Sadao Fugivara, Andressa Vilas Boas Nogueira, James Deschner, Joni Augusto Cirelli

{"title":"Sustained release of Sr and Ca from a micronanotopographic titanium surface improves osteoblast function","authors":"Luan Viana Faria, Maria Eduarda Scordamaia Lopes, Diego Pedreira de Oliveira, Fernando Santos da Silva, Cecilio Sadao Fugivara, Andressa Vilas Boas Nogueira, James Deschner, Joni Augusto Cirelli","doi":"10.1007/s10534-025-00668-8","DOIUrl":"10.1007/s10534-025-00668-8","url":null,"abstract":"<div>The surface chemistry and topographical features of dental implants play a crucial role in influencing the osseointegration process. Alkaline earth elements such as strontium (Sr) and calcium (Ca) exert beneficial effects in promoting bone formation. This study aimed to evaluate micronanotopographic cpTi substrates that doped these elements, Sr and Ca. The composition and morphology were analyzed by X-ray photoelectron spectroscopy, scanning electron microscopy (SEM), and atomic force microscopy (AFM). Wettability assays, Sr and Ca release tests, and electrochemical behavior were also conducted. Proliferation, adhesion, and differentiation of MC3T3-E1 cells on this surface were evaluated in vitro. Direct fluorescence assays and SEM, cell viability, alkaline phosphatase (ALP) activity, and mineralization nodule formation were performed. The biological results showed the absence of cytotoxicity after the treatments, increased cell spreading on the micronanotopographic substrates, and greater mineralization nodule formation on surfaces doped with Sr and Sr/Ca. Gene and protein expression of osteoblastic markers were assessed through PCR and ELISA, and some genes were regulated on the doped surfaces at three and seven days of cell culture, like Bglap, Ibsp, Spp, Col1a1, and Runx2. The micronanotopographic substrates modified the physicochemical properties and morphology of the pre-osteoblasts. The results indicate that the biological effect of implants treated with Sr and Sr/Ca was significantly superior to that of polished surfaces and undoped micronanotopographic implants. Furthermore, the addition of Sr alone was sufficient to improve events related to osseointegration.</div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":"38 2","pages":"623 - 646"},"PeriodicalIF":4.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In memoriam: Carl J. Carrano : July 14, 1950-January 26, 2022. 纪念：卡尔·j·卡拉诺：1950年7月14日- 2022年1月26日。

IF 4.1 3区生物学

Biometals Pub Date : 2025-03-12 DOI: 10.1007/s10534-024-00651-9

Frithjof C Küpper, Eleanor Carrano, Kenneth N Raymond, Eric Miller, Ricardo Cruz-López, Ana Mijovilovich, Kyoko Yarimizu, Andrew Cooksy, Wes Harris, Shady A Amin, Alison Butler, John C Carrano

引用次数: 0