Biometals最新文献

{"title":"Barley metallothionein isoforms, MT2b2 and MT4, differentially respond to photohormones in barley aleurone layer and their recombinant forms show different affinity for binding to zinc and cadmium","authors":"Zahra Pourjalali,&nbsp;Azar Shahpiri,&nbsp;Pooran Golkar","doi":"10.1007/s10534-022-00452-y","DOIUrl":"10.1007/s10534-022-00452-y","url":null,"abstract":"<div><p>Metallothioneins (MTs) are metal-binding proteins that have important roles in the homeostasis of heavy metals. In this study, the two MT genes was studied in response to phytohormones using the barley aleurone layer as a kind of model system. The aleurone layer was isolated from barley embryo-less half grains and was incubated for 24 h with different phytohormones. Based on the results the genes encoding HvMT2b2 and HvMT4 were down-regulated through gibberellic acid (GA), while they were and up-regulated through salicylic acid (SA). Despite this, these two genes were differentially expressed to other hormones. Furthermore, the proteins HvMT2b2 and HvMT4 were heterologous expressed as GST-fusion proteins in <i>E. coli</i>. The HvMT4 and HvMT2b2 heterologous expression in <i>E. coli</i> gives rise to 10- and 3-fold improvements in the accumulation capacity for Zn²⁺, respectively. Whereas the transgenic <i>E. coli</i> strain that expresses HvMT2b2 could accumulate Cd²⁺ three-fold higher than control. The expression of HvMT4 did not affect the accumulation of Cd²⁺. HvMT4 which is known as seed-specific isoform seems to be able to bind to Zn²⁺ with good affinity and cannot bind Cd²⁺. In comparison, HvMT2b2 was able to bind both Zn²⁺ and Cd²⁺. Therefore HvMT4 could serve a noteworthy role in zinc storage in barley seeds. The expression of HvMT4 is induced by SA 30-fold, concerning the untreated aleurone layer. Such results could provide good insights for the assessment of the effects of phytohormones in the molecular mechanism involved in essential metal storage in cereal seeds.</p></div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":"36 1","pages":"3 - 18"},"PeriodicalIF":3.5,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5171093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metals in seston from Cabo Frio Bay, a region under the influence of upwelling in SE–Brazil","authors":"Vinicius Tavares Kütter,&nbsp;Ana Luiza Spadano Albuquerque,&nbsp;Josino Costa Moreira,&nbsp;Eduardo Vianna de Almeida,&nbsp;Orangel Aguilera,&nbsp;Emmanoel Vieira Silva Filho","doi":"10.1007/s10534-022-00457-7","DOIUrl":"10.1007/s10534-022-00457-7","url":null,"abstract":"<div><p>This study, performed during 2003–2005 and 2008–2009, investigated metals (Al, Fe, Ba, Zn, Mn, Cr, Cu, Ni, Cd) content in seston at Cabo Frio Bay, SE–Brazil. This study may serve as a baseline of seston metal distribution to guide biogeochemical and ecological models. The seston fractions (&gt; 20 µm, &gt; 64 µm, &gt; 100 µm, and &gt; 150 µm) were sampled in sub-surface horizontal hauls. Metals were determined by ICP-MS. The metals range: Al (62.5–56,867.6 µg g⁻¹), Fe (23.5–25,384.0 µg g⁻¹), Mn (2.7–336.8 µg g⁻¹), Ba (&lt; 0.005–356.3 µg g⁻¹), Zn (0.5–94.2 µg g⁻¹), Cr (0.7–35.5 µg g⁻¹), Cu (4.3–41.7 µg g⁻¹), Ni (&lt; 0.005–19.1 µg g⁻¹) and Cd (&lt; 0.0004–2.4 µg g⁻¹). Aluminium, Fe, Mn, Ba, and Zn showed significant differences (p &lt; 0.05) between the seston fraction. The results obtained in this study suggest that the elements (Fe, Mn, Ba) in the seston were strongly influenced by the abiogenic source. In contrast, Zn, Cd, Cu, Cr, and Ni can be biogenic/anthropogenic sources. The significant positive linear correlation of Zn:P, Cd:P, and Cu:P can indicate an intracellular concentration higher than the external adsorption in the plankton community.</p></div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":"36 1","pages":"49 - 66"},"PeriodicalIF":3.5,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4984260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Speciation analysis of manganese against the background of its different content in the blood serum of dairy cows","authors":"S. V. Notova,&nbsp;S. V. Lebedev,&nbsp;O. V. Marshinskaia,&nbsp;T. V. Kazakova,&nbsp;O. P. Ajsuvakova","doi":"10.1007/s10534-022-00456-8","DOIUrl":"10.1007/s10534-022-00456-8","url":null,"abstract":"<div><p>Studies in the field of microelement speciation in the body of farm animals, in particular dairy cattle, are almost completely absent. The average concentration of Mn in the blood serum of all the studied animals (n = 80) was 2.5 μg/L, which corresponds to normal values. Of the total number of animals, 21% were the cows with the low normal values (serum Mn concentration ≤ 2 µg/L, i.e. less than Q₂₅ of the total sample) and 25% were the animals with the high normal values (serum Mn concentration ≥ 2.72 µg/L, i.e. more than Q₇₅ of the total sample). The data obtained in the course of the study indicate that the change in the Mn level, even in the range of normal values, is accompanied by the redistribution of this element over various protein fractions. The six found Mn blood serum forms are presumably represented by α2-macroglobulin (tetramer, dimer, and monomer), transferrin/albumine, manganese citrates, and “free” metal ions. The analyzed fractions of Mn found in the blood serum of cows had the following hierarchy of concentrations: in the group with low-normal values of Mn (“free” Mn &gt;&gt; tetrameric form of α2-macroglobulin &gt;&gt; transferrin/albumine &gt;&gt; dimeric form of α2-macroglobulin &gt;&gt; monomeric form of α2-macroglobulin &gt;&gt; citrate), in the group with high normal manganese values (\"free\" Mn &gt;&gt; monomeric form of α2-macroglobulin &gt;&gt; transferring/albumine &gt;&gt; citrate &gt;&gt; tetrameric form of α2-macroglobulin &gt;&gt; dimeric form of α2-macroglobulin). In the group with high normal Mn values relative to the group with low normal values, there was a percentage decrease in the tetrameric fraction of a2-macroglobulin from 17.2 to 4.4%, dimeric fraction of a2-macroglobulin from 6.9 to 2.2%, “free” Mn from 54.3 to 44.4% and an increase in monomeric fraction of a2-macroglobulin from 6.7 to 23.1%, transferrin/albumine from 10.1 to 17.7%, citrate from 4.8 to 8.2%. Our data demonstrate the features of Mn redistribution of dairy cows, which can be used for an extended assessment of the microelement status of animals.\u0000</p></div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":"36 1","pages":"35 - 48"},"PeriodicalIF":3.5,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10534-022-00456-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4986933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid identification of pyoverdines of fluorescent Pseudomonas spp. by UHPLC-IM-MS","authors":"Karoline Rehm,&nbsp;Vera Vollenweider,&nbsp;Rolf Kümmerli,&nbsp;Laurent Bigler","doi":"10.1007/s10534-022-00454-w","DOIUrl":"10.1007/s10534-022-00454-w","url":null,"abstract":"<div><p>Siderophores are iron-chelating molecules produced by bacteria and other microbes. They are involved with virulence in infections and play key roles in bacterial community assembly and as plant protectants due to their pathogen control properties. Although assays exist to screen whether newly isolated bacteria can produce siderophores, the chemical structures of many of these bio-active molecules remain unidentified due to the lack of rapid analytical procedures. An important group of siderophores are pyoverdines. They consist of a structurally diverse group of chromopeptides, whose amino acid sequence is characteristic for the fluorescent <i>Pseudomonas</i> species that secrets them. Although over 60 pyoverdine structures have been described so far, their characterization is cumbersome and several methods (isoelectrofocusing, iron uptake measurement, mass determination) are typically combined as ambiguous results are often achieved by a single method. Those additional experiments consume valuable time and resources and prevent high-throughput analysis. In this work, we present a new pyoverdine characterisation option by recording their collision cross sections (CCS) using trapped ion mobility spectrometry. This can be done simultaneously in combination with UHPLC and high-resolution MS resulting in a rapid identification of pyoverdines. The high specificity of CCS values is presented for 17 pyoverdines secreted by different <i>Pseudomonas</i> strains. The pyoverdine mass determination by full scan MS was supported by fragments obtained from broadband collision induced dissociation (bbCID). As iron contaminations in laboratories are not uncommon, CCS values of ferripyoverdines were also evaluated. Thereby, unusual and highly characteristic ion mobility patterns were obtained that are suitable as an alternative identification marker.</p></div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":"36 1","pages":"19 - 34"},"PeriodicalIF":3.5,"publicationDate":"2022-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10534-022-00454-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4804916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization and expression analysis of selenoprotein W gene in rainbow trout (Oncorhynchus mykiss) with dietary selenium levels","authors":"Chenlei Liao,&nbsp;Feng Zhang,&nbsp;Zhenlei Teng,&nbsp;Guirong Zhang,&nbsp;Ying Yang,&nbsp;Pengke Xu,&nbsp;Xixuan Huang,&nbsp;Li Wang,&nbsp;Fan Yang,&nbsp;Zhilong Yang,&nbsp;Xuezhen Zhang","doi":"10.1007/s10534-022-00451-z","DOIUrl":"10.1007/s10534-022-00451-z","url":null,"abstract":"<div><p>Selenium (Se) plays an essential role in the growth of fish and performs its physiological functions mainly through incorporation into selenoproteins. Our previous studies suggested that the selenoprotein W gene (<i>selenow</i>) is sensitive to changes in dietary Se in rainbow trout. However, the molecular characterization and tissue expression pattern of <i>selenow</i> are still unknown. Here, we revealed the molecular characterization, the tissue expression pattern of rainbow trout <i>selenow</i> and analyzed its response to dietary Se. The open reading frame (ORF) of the <i>selenow</i> gene was composed of 393 base pairs (bp) and encodes a 130-amino-acid protein. The 3′ untranslated region (UTR) was 372 bp with a selenocysteine insertion sequence (SECIS) element. Remarkably, the rainbow trout <i>selenow</i> gene sequence was longer than those reported for mammals and most other fish. A β1-α1-β2-β3-β4-α2 pattern made up the secondary structure of SELENOW. Furthermore, multiple sequence alignment revealed that rainbow trout SELENOW showed a high level of identity with SELENOW from <i>Salmo salar</i>. In addition, the <i>selenow</i> gene was ubiquitously distributed in 13 tissues with various abundances and was predominantly expressed in muscle and brain. Interestingly, dietary Se significantly increased <i>selenow</i> mRNA expression in muscle. Our results highlight the vital role of <i>selenow</i> in rainbow trout muscle response to dietary Se levels and provide a theoretical basis for studies of <i>selenow</i>.</p></div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":"35 6","pages":"1359 - 1370"},"PeriodicalIF":3.5,"publicationDate":"2022-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4805237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multielement determination (essential and potentially toxic elements) in eye shadows exposed to consumption in Brazil using ICP OES","authors":"Cinira Mello Santana,&nbsp;Thaís Luz de Sousa,&nbsp;Abdon Luiz Ornelas Latif,&nbsp;Lorena Santos Lobo,&nbsp;Gleice Rayanne da Silva,&nbsp;Hemerson Iury Ferreira Magalhães,&nbsp;Mariângela Vieira Lopes,&nbsp;Clícia Maria de Jesus Benevides,&nbsp;Rennan Geovanny Oliveira Araujo,&nbsp;Daniele Cristina Muniz Batista dos Santos,&nbsp;Aníbal de Freitas Santos Júnior","doi":"10.1007/s10534-022-00444-y","DOIUrl":"10.1007/s10534-022-00444-y","url":null,"abstract":"<div><p>Worldwide, cosmetics (especially eye shadows) are widely consumed and have a great impact on the economy. The aim of this study was to determine the multielement composition, focusing on essential and potentially toxic elements, in cosmetics (eye shadow) exposed to consumption in Brazil. Concentrations of 17 elements (Al, As, Ba, Cd, Co, Cr, Cu, Mn, Mo, Ni, Pb, Sb, Se, Sr, Ti, V and Zn) were determined in samples (produced in China and Brazil) using a sequential optical emission spectrometer with inductively coupled plasma (ICP OES) after acid digestion, assisted by a closed digester block (6 mL of HNO₃ + 2 mL of H₂O₂ + 1 mL of Triton ×-100 + 1 mL of ultrapure water). The method was validated by linearity, precision, accuracy, limits of detection (LoD) and quantification (LoQ). The elements were quantified (in µg g⁻¹): Al (852–21,900), Ba (3.47–104), Cd (1.70–6.93), Cr (&lt; 8.53–66.6), Cu (&lt; 0.480–14.5), Mn (92.20–1,190), Ni (&lt; 4.23–40.7), Pb (&lt; 2.16–5.06), Sb (1.10–10.5), Sr (0.760–46.0), Ti (32.0–440), V (&lt; 0.85–1.7) and Zn (24.90–2,600). As, Co, Mo and Se in all the investigated samples were found to be below the LoQ values of ICP OES. In this study, regardless of sample compositions and origins (Brazilian or Chinese), high levels of Al, Cd, Cr, Cu, Mn, Ni, Pb, Sb, Ti, V and Zn were observed, exceeding the recommended maximum tolerable limits, according to Brazilian and global legislations, which may present potential risks to human health and the environment.</p><h3>Graphical abstract</h3>\u0000 <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\u0000 </div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":"35 6","pages":"1281 - 1297"},"PeriodicalIF":3.5,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10534-022-00444-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4734842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nonsense-mediated mRNA decay and metal ion homeostasis and detoxification in Saccharomyces cerevisiae","authors":"Xinyi Zhang,&nbsp;Bessie W. Kebaara","doi":"10.1007/s10534-022-00450-0","DOIUrl":"10.1007/s10534-022-00450-0","url":null,"abstract":"<div><p>The highly conserved Nonsense-mediated mRNA decay (NMD) pathway is a translation dependent mRNA degradation pathway. Although NMD is best known for its role in degrading mRNAs with premature termination codons (PTCs) generated during transcription, splicing, or damage to the mRNAs, NMD is now also recognized as a pathway with additional important functions. Notably, NMD precisely regulates protein coding natural mRNAs, hence controlling gene expression within several physiologically significant pathways. Such pathways affected by NMD include nutritional bio-metal homeostasis and metal ion detoxification, as well as crosstalk between these pathways. Here, we focus on the relationships between NMD and various metal homeostasis and detoxification pathways. We review the described role that the NMD pathway plays in magnesium, zinc, iron, and copper homeostasis, as well as cadmium detoxification.</p></div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":"35 6","pages":"1145 - 1156"},"PeriodicalIF":3.5,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10534-022-00450-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4738500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactoferrin: from the structure to the functional orchestration of iron homeostasis","authors":"Giusi Ianiro,&nbsp;Luigi Rosa,&nbsp;Maria Carmela Bonaccorsi di Patti,&nbsp;Piera Valenti,&nbsp;Giovanni Musci,&nbsp;Antimo Cutone","doi":"10.1007/s10534-022-00453-x","DOIUrl":"10.1007/s10534-022-00453-x","url":null,"abstract":"<div><p>Iron is by far the most widespread and essential transition metal, possessing crucial biological functions for living systems. Despite chemical advantages, iron biology has forced organisms to face with some issues: ferric iron insolubility and ferrous-driven formation of toxic radicals. For these reasons, acquisition and transport of iron constitutes a formidable challenge for cells and organisms, which need to maintain adequate iron concentrations within a narrow range, allowing biological processes without triggering toxic effects. Higher organisms have evolved extracellular carrier proteins to acquire, transport and manage iron. In recent years, a renewed interest in iron biology has highlighted the role of iron-proteins dysregulation in the onset and/or exacerbation of different pathological conditions. However, to date, no resolutive therapy for iron disorders has been found. In this review, we outline the efficacy of Lactoferrin, a member of the transferrin family mainly secreted by exocrine glands and neutrophils, as a new emerging orchestrator of iron metabolism and homeostasis, able to counteract iron disorders associated to different pathologies, including iron deficiency and anemia of inflammation in blood, Parkinson and Alzheimer diseases in the brain and cystic fibrosis in the lung.</p></div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":"36 3","pages":"391 - 416"},"PeriodicalIF":3.5,"publicationDate":"2022-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4439300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iron overload impairs renal function and is associated with vascular calcification in rat aorta","authors":"Yanqiu Song,&nbsp;Ning Yang,&nbsp;Hailong Si,&nbsp;Ting Liu,&nbsp;Hongyu Wang,&nbsp;Hua Geng,&nbsp;Qin Qin,&nbsp;Zhigang Guo","doi":"10.1007/s10534-022-00449-7","DOIUrl":"10.1007/s10534-022-00449-7","url":null,"abstract":"<div><p>Vascular calcification (VC) has been associated with a risk of cardiovascular diseases. Iron may play a critical role in progressive VC. Therefore, we investigated the effects of iron overload on the aorta of rats. A rat model of iron overload was established by intraperitoneal injection of Iron-Dextran. The levels of iron, calcium, and ALP activity were detected. Von Kossa staining and Perl’s staining were conducted. The expression of iron metabolism-related and calcification related factors were examined in the aortic tissue of rats. The results showed serum and aortic tissue iron were increased induced by iron overload and excessive iron induced hepatic and renal damage. In iron overload rats, the expression of divalent metal transporter 1 (DMT1) and hepcidin were higher, but ferroportin1 (FPN1) was lower. Von Kossa staining demonstrated calcium deposition in the aorta of iron overload rats. The calcium content and ALP activity in serum and aortic tissue were increased and iron level in aortic tissue highly correlated with calcium content and ALP activity. The expressions of the osteogenic markers were increased while a decrease of Alpha-smooth muscle actin (α-SMA) in the aortic tissue of iron overload rats. IL-24 was increased during the calcification process induced by iron. Overall, we demonstrated excessive iron accumulation in the aortic tissue and induced organs damage. The iron metabolism-related factors were significantly changed during iron overload. Moreover, we found that iron overload leads to calcium deposition in aorta, playing a key role in the pathological process of VC by mediating osteoblast differentiation factors.</p></div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":"35 6","pages":"1325 - 1339"},"PeriodicalIF":3.5,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10534-022-00449-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4043710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Albumin fusion with human lactoferrin shows enhanced inhibition of cancer cell migration","authors":"Hana Nopia,&nbsp;Daisuke Kurimoto,&nbsp;Atsushi Sato","doi":"10.1007/s10534-022-00447-9","DOIUrl":"10.1007/s10534-022-00447-9","url":null,"abstract":"<div><p>The fusion of human serum albumin (HSA) with human lactoferrin (hLF) (designated as hLF-HSA) has improved the pharmacokinetic properties and anti-proliferative activities of hLF against cancer cells. In this study, we evaluated the anti-migratory activities of hLF and hLF-HSA against the human lung adenocarcinoma PC-14 cell line using wound healing and Boyden chamber assays. Despite the unexpected hLF-induced migration, hLF-HSA clearly demonstrated the complete inhibition of PC-14 cell migration. To examine the mechanism underlying the enhanced PC-14 cell migration by hLF alone but suppressed migration by hLF-HSA, we focused on the matrix metalloproteinase (MMP) family of endopeptidases because MMPs are often reported to play important roles in facilitating the migration and metastasis of cancer cells. Furthermore, hLF is a transactivator of MMP1 transcription. As expected, treatment of cells with hLF and hLF-HSA led to the upregulation and downregulation of MMP1, respectively. In contrast, MMP9 expression levels, which are often associated with cancer migration, were unchanged in the presence of either protein. An MMP inhibitor attenuated hLF-induced migration of PC-14 cells. Therefore, specific enhancement and suppression of MMP1 expression by hLF and hLF-HSA have been implicated as causes of a marked increase and decrease in PC-14 cell migration, respectively. In conclusion, the fusion of HSA with hLF (hLF-HSA) promoted its anti-migratory effects against cancer cells. Therefore, hLF-HSA is a promising anti-cancer drug candidate based on its improved anti-migratory activity towards cancer cells.</p></div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":"36 3","pages":"629 - 638"},"PeriodicalIF":3.5,"publicationDate":"2022-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10534-022-00447-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5064756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}