Sustained release of Sr and Ca from a micronanotopographic titanium surface improves osteoblast function

IF 4.1 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Luan Viana Faria, Maria Eduarda Scordamaia Lopes, Diego Pedreira de Oliveira, Fernando Santos da Silva, Cecilio Sadao Fugivara, Andressa Vilas Boas Nogueira, James Deschner, Joni Augusto Cirelli
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Abstract

The surface chemistry and topographical features of dental implants play a crucial role in influencing the osseointegration process. Alkaline earth elements such as strontium (Sr) and calcium (Ca) exert beneficial effects in promoting bone formation. This study aimed to evaluate micronanotopographic cpTi substrates that doped these elements, Sr and Ca. The composition and morphology were analyzed by X-ray photoelectron spectroscopy, scanning electron microscopy (SEM), and atomic force microscopy (AFM). Wettability assays, Sr and Ca release tests, and electrochemical behavior were also conducted. Proliferation, adhesion, and differentiation of MC3T3-E1 cells on this surface were evaluated in vitro. Direct fluorescence assays and SEM, cell viability, alkaline phosphatase (ALP) activity, and mineralization nodule formation were performed. The biological results showed the absence of cytotoxicity after the treatments, increased cell spreading on the micronanotopographic substrates, and greater mineralization nodule formation on surfaces doped with Sr and Sr/Ca. Gene and protein expression of osteoblastic markers were assessed through PCR and ELISA, and some genes were regulated on the doped surfaces at three and seven days of cell culture, like Bglap, Ibsp, Spp, Col1a1, and Runx2. The micronanotopographic substrates modified the physicochemical properties and morphology of the pre-osteoblasts. The results indicate that the biological effect of implants treated with Sr and Sr/Ca was significantly superior to that of polished surfaces and undoped micronanotopographic implants. Furthermore, the addition of Sr alone was sufficient to improve events related to osseointegration.

Abstract Image

从微纳钛表面持续释放硒和钙可改善成骨细胞功能。
牙种植体的表面化学和地形特征对骨整合过程起着至关重要的作用。碱土元素如锶(Sr)和钙(Ca)对促进骨形成有有益作用。本研究旨在评价掺杂这些元素,Sr和Ca的cpTi衬底的微形貌。通过x射线光电子能谱,扫描电子显微镜(SEM)和原子力显微镜(AFM)分析了其组成和形貌。并进行了润湿性测试、Sr和Ca释放测试以及电化学行为测试。体外观察MC3T3-E1细胞在该表面的增殖、粘附和分化情况。进行了直接荧光测定和扫描电镜、细胞活力、碱性磷酸酶(ALP)活性和矿化结节形成。生物学结果表明,处理后细胞毒性消失,细胞在微纳米基质上扩散增加,并且在掺杂Sr和Sr/Ca的表面上形成更大的矿化结节。通过PCR和ELISA检测成骨细胞标志物的基因和蛋白表达,并在细胞培养3天和7天时在掺杂表面调控部分基因,如Bglap、Ibsp、Spp、Col1a1、Runx2。微形貌基质改变了前成骨细胞的物理化学性质和形态。结果表明,Sr和Sr/Ca处理的微纳米形貌植入物的生物效应明显优于抛光表面和未掺杂的微纳米形貌植入物。此外,仅添加锶就足以改善与骨整合相关的事件。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biometals
Biometals 生物-生化与分子生物学
CiteScore
5.90
自引率
8.60%
发文量
111
审稿时长
3 months
期刊介绍: BioMetals is the only established journal to feature the important role of metal ions in chemistry, biology, biochemistry, environmental science, and medicine. BioMetals is an international, multidisciplinary journal singularly devoted to the rapid publication of the fundamental advances of both basic and applied research in this field. BioMetals offers a forum for innovative research and clinical results on the structure and function of: - metal ions - metal chelates, - siderophores, - metal-containing proteins - biominerals in all biosystems. - BioMetals rapidly publishes original articles and reviews. BioMetals is a journal for metals researchers who practice in medicine, biochemistry, pharmacology, toxicology, microbiology, cell biology, chemistry, and plant physiology who are based academic, industrial and government laboratories.
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