Journal of Periodontal and Implant Science最新文献

{"title":"Identification of susceptibility modules and genes for peri-implantitis compared to periodontitis within the same host environment using weighted gene co-expression network analysis.","authors":"Ju-Young Lee, Yeongjoo Kim, Jung-Min Oh, Yun Hak Kim, Hyun-Joo Kim","doi":"10.5051/jpis.2401500075","DOIUrl":"https://doi.org/10.5051/jpis.2401500075","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to identify new susceptibility modules and genes by analyzing the transcriptional profiles of peri-implantitis and periodontitis within the same host environment, using weighted gene co-expression network analysis (WGCNA).</p><p><strong>Methods: </strong>Gingival tissue samples were collected from 10 patients, each presenting with both periodontitis and peri-implantitis sites, and were used for RNA sequencing. We conducted WGCNA to identify key modules that showed distinct transcriptional expression profiles between periodontitis and peri-implantitis. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses were carried out using R software. Genes with an adjusted <i>P</i> value greater than 0.05 were excluded from gene selection using the Pearson correlation method.</p><p><strong>Results: </strong>A total of 2,226 regulated genes were identified, and those with similar expression patterns were grouped into 5 color-coded functional modules using WGCNA. Among these, 3 modules showed distinct differences in expression profiles between peri-implantitis and periodontitis. The turquoise and yellow modules were associated with upregulation in peri-implantitis, while the blue module was linked to periodontitis. This finding suggests that peri-implantitis and periodontitis have significantly different transcriptional signatures. Over-representation analysis was conducted to explore the component genes of the established modules. The top-ranked genes, selected based on their network connectivity within the modules, were identified using DESeq2 and were considered hub genes.</p><p><strong>Conclusions: </strong>WGCNA revealed distinct modular gene patterns in peri-implantitis and periodontitis, highlighting transcriptional differences between the 2 conditions. Notably, we identified 10 key genes from each of the 3 modules-the blue module associated with periodontitis-dominant pathways, and the turquoise and yellow modules associated with peri-implantitis-dominant pathways. The hub genes and pathways unveiled in this research are likely key contributors to the progression of peri-implantitis and warrant further exploration as promising candidates.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term clinical and radiographic outcomes of a bone-level, 2-piece, internal connection implant system with coronal microthreads over 10 years of follow-up: a retrospective clinical study.","authors":"Young Woo Song, Seung Ha Yoo, Jin-Young Park, Jae-Kook Cha, Jeong-Won Paik, Jung-Seok Lee, Daniel S Thoma, Ui-Won Jung","doi":"10.5051/jpis.2401100055","DOIUrl":"https://doi.org/10.5051/jpis.2401100055","url":null,"abstract":"<p><strong>Purpose: </strong>This study retrospectively investigated the long-term clinical and radiographic outcomes of a bone-level type, 2-piece, internal connection dental implant system characterized by coronal microthreads.</p><p><strong>Methods: </strong>A total of 872 implants placed in 284 patients were selected from 1,845 implants placed in 691 patients by experienced periodontists at Yonsei University Dental Hospital. These selected implants had been followed up for over 10 years and were included in the present study. A statistical evaluation of implant survival and treatment success, based on changes in marginal bone levels, was conducted using electronic records and consecutively taken radiographs.</p><p><strong>Results: </strong>Over a follow-up period of 12.3±2.0 years, 830 of the 872 implants remained intact, yielding a cumulative survival rate of 95.2% at the implant level and 88.4% at the patient level. The cumulative treatment success rates, characterized by marginal bone loss of less than 2 mm, stood at 87.0% for implants and 76.1% for patients. Among the 830 surviving implants, 113 fixtures were classified as ailing, with an average marginal bone loss of 4.09±1.44 mm. Cox regression analysis revealed that implants 8 mm in length or shorter were significantly more likely to fail or experience pathologic marginal bone resorption, with hazard ratios of 3.71 and 2.00, respectively (<i>P</i><0.05).</p><p><strong>Conclusions: </strong>The survival and treatment success of the investigated microthreaded, bone-level, 2-piece, internal connection implants were acceptable over a follow-up period exceeding 10 years. However, shorter dental implants exhibited a higher propensity for failure and excessive marginal bone loss.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term assessment of a modified tunneling technique for root coverage in lower anterior gingival recession: a retrospective study.","authors":"Sungtae Kim, Hee-Seung Han, Hyunkyung Kim, Hyunjae Kim, Yang-Jo Seol, Young-Dan Cho","doi":"10.5051/jpis.2402660133","DOIUrl":"https://doi.org/10.5051/jpis.2402660133","url":null,"abstract":"<p><strong>Purpose: </strong>Root coverage (RC) procedures require long-term evaluation. This study assessed the clinical validity and long-term stability of a modified tunneling technique for lower anterior gingival recession (GR) using a subepithelial connective tissue graft (SCTG) and a volume-stable collagen matrix.</p><p><strong>Methods: </strong>Across 39 patients, 66 mandibular incisors with ≥1.0 mm of GR were examined before and after RC surgery. Clinical photographs documenting the results of RC were taken at baseline (T₀) and the most recent follow-up visit (T<i>₁</i>). Impressions were obtained either at baseline (T₀) or 3 weeks later (T₃). The recession depth, Miller classification, and rates of RC and complete root coverage (CRC) were assessed.</p><p><strong>Results: </strong>This study analyzed 66 GR sites across 39 patients, with an average follow-up period of 41.3 months. Overall, the mean RC achieved was 86.2%±15.7%. Among single recessions, the RC was 85.2%±25.6% for Miller class I, 91.5%±10.4% for class II, and 79.2%±18.3% for class III. Regarding multiple recessions, the RC was 85.1%±16.2% for Miller class I, 87.0%±12.5% for class II, and 89.8%±16.0% for class III. By Miller classification, the RC was 85.1%±16.8% for class I, 88.7%±11.6% for class II, and 85.8%±17.3% for class III. Furthermore, the RC varied by follow-up duration: 72.5%±15.1% at 12 months, 90.1%±12.6% at 25-36 months, 89.0%±16.7% at 37-48 months, 91.10%±9.88% at 49-60 months, and 97.6±4.79% for longer than 61 months, with 77.8% of the last group achieving CRC. RC also differed based on the initial recession depth, at 88.0%±16.8% for 1-3 mm, 83.1%±14.1% for 3-6 mm, and 80.2%±5.04% for depths exceeding 6 mm.</p><p><strong>Conclusions: </strong>A modified tunneling technique, utilizing SCTG and a volume-stable collagen matrix, appears to represent a reliable option for the long-term management of GR in the lower anterior region, even in cases involving multiple Miller class III GRs.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The development and validation of a Korean version of the oral hygiene-related self-efficacy tool.","authors":"Soo-Auk Park, Eun-Ae Kim, Jae-Young Lee","doi":"10.5051/jpis.2401780089","DOIUrl":"https://doi.org/10.5051/jpis.2401780089","url":null,"abstract":"<p><strong>Purpose: </strong>Self-efficacy is an important factor in the management of chronic oral diseases. This study aimed to develop a Korean version of a self-efficacy tool related to personal oral hygiene management, and verify its validity and reliability.</p><p><strong>Methods: </strong>This study evaluated the validity and reliability of a Korean version of the oral health-related self-efficacy measurement tool (OHSE-K). The sub-factors of this self-efficacy tool are tooth brushing, interdental hygiene management, and dental visits. The original items were translated into Korean, and their content validity was confirmed. Initially, a preliminary survey was conducted, followed by the main survey. The main survey comprised 19 content-verified items. The validity and reliability of the main survey were evaluated through repeated exploratory factor analyses. A randomly selected sample of Korean adults, aged 19 years or older, completed the OHSE-K online between May 10 and June 2, 2023. The study recruited 400 adults for the preliminary survey. Data were analyzed using PASW 25.0.</p><p><strong>Results: </strong>The OHSE-K demonstrated a high level of overall reliability (Cronbach's α=0.891). Exploratory factor analysis revealed 3 significant factors: tooth-brushing self-efficacy, interdental hygiene management self-efficacy, and dental visit self-efficacy, with a cumulative explanation rate of 65.114%. The criterion validity results indicated that oral healthcare awareness and behavior, as well as unmet dental treatment needs, were significant (<i>P</i><0.05). Additionally, the OHSE-K scores showed significant correlations with all 3 sub-factors (<i>P</i><0.05).</p><p><strong>Conclusions: </strong>The OHSE-K is a reliable tool. Our results demonstrated its validity and reliability.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does defect configuration affect the outcomes of alveolar ridge preservation? An experimental <i>in vivo</i> study.","authors":"Dongseob Lee, Jin Uk Choi, Young-Chang Ko, Ki-Tae Koo, Yang-Jo Seol, Yong-Moo Lee, Jungwon Lee","doi":"10.5051/jpis.2401480074","DOIUrl":"https://doi.org/10.5051/jpis.2401480074","url":null,"abstract":"<p><strong>Purpose: </strong>The purpose of this study was to compare the bone healing potential of 1-, 2-, and 3-wall defects following alveolar ridge preservation (ARP) treatment, as well as to evaluate the efficacy of ARP as a treatment option for destructive sites.</p><p><strong>Methods: </strong>Three groups, characterized by 1-, 2-, and 3-wall defects, were randomly assigned to the maxillary second, third, and fourth premolars in each of 8 beagle dogs. Each defect was created at either the mesial or distal root site of the tooth, which was hemi-sectioned and extracted. The contralateral root was preserved to superimpose with the experimental site for histomorphometric analysis. For each site, either spontaneous healing (SH; control) or ARP (test intervention) was randomly applied. Each group was divided in half and underwent a healing period of either 4 or 12 weeks. The Mann-Whitney <i>U</i> test and Kruskal-Wallis test were used for histomorphometric analyses. Statistical significance was set at <i>P</i><0.05.</p><p><strong>Results: </strong>Qualitative analysis revealed a higher percentage of new bone in the apical area compared to the coronal area, regardless of defect type and healing period. In quantitative analysis, the 3-wall defect exhibited a significantly higher percentage of mineralization in the ARP group after 12 weeks of healing (ARP: 61.73%±7.52%; SH: 48.84%±3.06%; <i>P</i>=0.029). An increased percentage of mineralization was observed with a greater number of remaining bony walls, although this finding did not reach statistical significance.</p><p><strong>Conclusions: </strong>Within the limitations of this study, ARP treatment for compromised sockets appears to yield a higher percentage of mineralization compared to SH. Although the effectiveness of the remaining bony walls was limited, their presence appeared to improve the percentage of mineralization in ARP treatment.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142510971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The importance of international collaboration in periodontology and global leadership.","authors":"Jeong-Ho Yun","doi":"10.5051/jpis.245405edi01","DOIUrl":"10.5051/jpis.245405edi01","url":null,"abstract":"","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":"54 5","pages":"293-294"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11543330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone regeneration using activin A/BMP2 chimera (AB204) with collagen membrane in rats with calvarial defects.","authors":"Haeji Yum, Hee-Seung Han, Jung-Tae Lee, Young-Dan Cho, Sungtae Kim","doi":"10.5051/jpis.2303820191","DOIUrl":"10.5051/jpis.2303820191","url":null,"abstract":"<p><strong>Purpose: </strong>Collagen has long been recognized as an excellent carrier for growth factors, and membrane-type collagen has been widely applied in dentistry for guided bone regeneration. This study was conducted to examine the effects of an activin A/BMP2 chimera (AB204) combined with a collagen membrane (CM) on bone repair in a rat calvarial defect model.</p><p><strong>Methods: </strong>A unilateral calvarial defect measuring 5.0 mm was surgically created in 32 Sprague-Dawley rats. The rats were then randomly assigned to 1 of 4 groups, each consisting of 8 animals: control (untreated), CM (treated with a CM only), CM/bone morphogenetic protein 2 (BMP2) (treated with a CM and 1.0 μg of BMP2), and CM/AB204 (treated with a CM and 1.0 μg of AB204). Bone regeneration was evaluated using micro-computed tomography (CT) and histological analysis at 2 and 4 weeks following surgery.</p><p><strong>Results: </strong>Micro-CT analysis revealed that bone formation in the CM/BMP2 and CM/AB204 groups was superior to that observed in the control and CM groups at both 2 and 4 weeks postoperatively. BMP2 induced greater bone regeneration than AB204 at 2 weeks; however, AB204 resulted in a greater bone volume at 4 weeks, achieving the highest values recorded. No significant differences were found between the CM/BMP2 and CM/AB204 groups at either time point (<i>P</i>>0.05). On histological examination, new bone formation was evident in both CM/BMP2 and CM/AB204 groups.</p><p><strong>Conclusions: </strong>Within the limitations of this study, the findings indicate that AB204 may enhance osteogenic potential when used in combination with CM for bone regeneration.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":" ","pages":"309-321"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11543331/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive treatment protocol for peri-implantitis: an up-to date narrative review of the literature.","authors":"Inpyo Hong, Ki-Tae Koo, Sang-Yoon Oh, Hwee Woong Park, Ignacio Sanz-Martín, Jae-Kook Cha","doi":"10.5051/jpis.2303360168","DOIUrl":"10.5051/jpis.2303360168","url":null,"abstract":"<p><p>This narrative review describes up-to-date treatment options for peri-implantitis and proposes a treatment protocol and flowchart based on the current scientific evidence. Peri-implantitis treatment should be based on the phased treatment protocol for periodontitis, which is a continuous flow of decisions for extraction, nonsurgical and surgical treatments with step-by-step re-evaluation. The protocol's goals are to fulfill the success criteria for peri-implantitis treatment (probing depth of ≤5 mm, and absence of bleeding on probing, suppuration, and progressive bone loss) and to halt disease progression. Fixtures with peri-implantitis can initially be classified as failed or failing. A failed implant needs to be removed. In contrast, nonsurgical and surgical treatments can be applied to a failing implant. Nonsurgical treatment should be the initial treatment for failing implants; however, sole nonsurgical treatment was regarded as inefficient for peri-implantitis. Recent studies have found that the adjunctive use of antibiotics to nonsurgical debridement increased the success of nonsurgical treatment for peri-implantitis. Surgical treatments can be classified into resective, access, and reconstructive surgeries. The technique should be selected according to the patient's bone defect configuration, which relate to regenerative potential. Various combinations of decontamination methods (e.g., mechanical, chemical, and pharmacological approaches) are required to achieve absolute surface decontamination. Clinicians should select an appropriate surface decontamination strategy according to the purpose of surgery. After signs of disease disappear and its progression is halted through active peri-implantitis treatment, it is necessary to enroll patients into maintenance programs. Compliance of patients with the maintenance program reduces the recurrence of peri-implantitis and sustains clinical success after treatment. Maintenance visits should include professional plaque control and hygiene care reinforcement for patients, and their interval should be set according to individual peri-implantitis risk. Clinicians should remind that peri-implantitis treatment is not a single procedure, but rather a continuing cycle of treatment and re-evaluation.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":" ","pages":"295-308"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11543328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139643199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The activin/BMP-2 chimera AB204 promotes periodontal tissue regeneration in a buccal dehiscence model: a pilot study.","authors":"Hee-Seung Han, Jung-Tae Lee, Young-Dan Cho, Sungtae Kim","doi":"10.5051/jpis.2303600180","DOIUrl":"10.5051/jpis.2303600180","url":null,"abstract":"<p><strong>Purpose: </strong>A combination of activin and bone morphogenetic protein-2 (BMP-2), termed AB204, has been shown to improve osteogenic potential with fewer side effects than BMP-2 alone. This study was performed to evaluate the effect of AB204 on periodontal tissue regeneration in a dog buccal dehiscence model.</p><p><strong>Methods: </strong>Buccal dehiscence defects were created on the maxillary premolars (P1, P2, and P3) of 6 mongrel dogs. After 5 weeks, the dogs were randomly assigned to 1 of 3 groups: the control, collagen matrix (CM), and CM/AB204 groups. Grafting procedures were then performed. The dogs were sacrificed 8 weeks after the grafting procedure, and volumetric and histological analyses were conducted.</p><p><strong>Results: </strong>The thickness of the buccal gingiva in the CM/AB204 group was greater than those in the other groups at 2 weeks (<i>P</i><0.05). The ridge width in the AB204/CM group exceeded the width in the other groups at 4 and 8 weeks; however, the difference was not statistically significant. Histological analysis revealed that the CM/AB204 group demonstrated the formation of new bone surrounded by newly formed periodontal ligament and cementum (<i>P</i>=0.035).</p><p><strong>Conclusions: </strong>The combined application of CM and AB204 shows promise in facilitating the regeneration of periodontal attachment, including the formation of new bone, cementum, and periodontal ligament.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":" ","pages":"322-335"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11543333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140897326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guided bone regeneration of calcium phosphate-coated and strontium ranelate-doped titanium mesh in a rat calvarial defect model.","authors":"Seon Mi Byeon, Tae Sung Bae, Min Ho Lee, Seung Geun Ahn","doi":"10.5051/jpis.2303000150","DOIUrl":"10.5051/jpis.2303000150","url":null,"abstract":"<p><strong>Purpose: </strong>When applied alone, titanium (Ti) mesh may not effectively block the penetration of soft tissues, resulting in insufficient new bone formation. This study aimed to confer bioactivity and improve bone regeneration by doping calcium phosphate (CaP) precipitation and strontium (Sr) ranelate onto a TiO₂ nanotube (TNT) layer on the surface of a Ti mesh.</p><p><strong>Methods: </strong>The TNT layer was obtained by anodizing on the Ti mesh, and CaP was formed by cyclic pre-calcification. The final specimens were produced by doping with Sr ranelate. The surface properties of the modified Ti mesh were investigated using high-resolution field emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray diffraction. To evaluate the effects of surface treatment on cell viability, osteoblasts were cultured for 1-3 days, and their absorbance was subsequently measured. In an <i>in vivo</i> experiment, critical-size defects were created in rat calvaria (Ф=8 mm). After 5 weeks, the rats were sacrificed (n=4 per group) and bone blocks were taken for micro-computed tomography and histological analysis.</p><p><strong>Results: </strong>After immersing the Sr ranelate-doped Ti mesh in simulated body fluid, the protrusions observed in the initial stage of hydroxyapatite were precipitated as a dense structure. On day 3 of osteoblast culture, cell viability was significantly higher on the pre-calcified Sr ranelate-doped Ti mesh surface than on the untreated Ti mesh surface (<i>P</i><0.05). In the <i>in vivo</i> experiment, a bony bridge formed between the surrounding basal bone and the new bone under the Sr ranelate-doped Ti mesh implanted in a rat calvarial defect, closing the defect. New bone mineral density (0.91±0.003 g/mm³) and bone volume (29.35±2.082 mm³) significantly increased compared to the other groups (<i>P</i><0.05).</p><p><strong>Conclusions: </strong>Cyclic pre-calcification of a Ti mesh with a uniform TNT layer increased bioactivity, and subsequent doping with Sr ranelate effectively improved bone regeneration in bone defects.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":" ","pages":"336-348"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11543329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139643200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}