Journal of Periodontal and Implant Science最新文献

{"title":"Herbal-derived bioactive compounds in alveolar bone regeneration: a narrative review.","authors":"Nguyen Pham-Hanh Luong, Kyoung-Hwa Kim, Yang-Jo Seol","doi":"10.5051/jpis.2600740037","DOIUrl":"https://doi.org/10.5051/jpis.2600740037","url":null,"abstract":"<p><p>Adequate alveolar bone volume is essential for successful implant therapy; however, current regenerative approaches are limited by donor-site morbidity, the biological passivity of bone substitutes, and safety concerns surrounding high-dose growth factors. This narrative review evaluates the potential of herbal-derived bioactive compounds to address these limitations through targeted osteoimmunomodulation, with particular emphasis on establishing a microenvironment conducive to predictable alveolar bone regeneration. The biological mechanisms of five major classes of herbal-derived bioactive compounds-flavonoids, polyphenols, terpenoids, alkaloids, and polysaccharides-were examined through a literature search of English-language articles published between 2000 and 2025. The review focused on their capacity to resolve inflammation, promote angiogenesis, and stimulate osteogenesis. Current evidence suggests that these agents exert pleiotropic effects that may improve nonpermissive recipient sites and restore a healing microenvironment favorable to bone formation. The literature also indicates a shift from compositionally variable crude extracts toward chemically defined and structurally modified compounds. QG3030 exemplifies this approach as a lappaconitine derivative designed to enhance osteoinductive potency while reducing systemic toxicity. Despite encouraging <i>in vitro</i> and <i>in vivo</i> findings, a substantial translational gap remains because few high-quality clinical trials have evaluated quantifiable hard-tissue outcomes. Herbal-derived bioactive compounds may therefore provide a useful means of biofunctionalizing conventional scaffolds. Future studies should prioritize the integration of chemically defined agents with stimuli-responsive biomaterials to achieve spatiotemporally controlled drug release and determine whether preclinical efficacy can be translated into predictable alveolar bone regeneration.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149138881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of smoking cessation on periodontal regeneration therapies: a propensity score-matched study.","authors":"Yoshito Ochiai, Yumi Saito, Misaki Matsuzaki, Takumi Morita, Kazuma Igarashi, Masatomo Wada, Arisa Yamaguchi, Mizuho Yamazaki-Takai, Hideki Takai, Yorimasa Ogata, Nozomi Ogayama, Masayuki Sakamoto, Yohei Nakayama","doi":"10.5051/jpis.2602840142","DOIUrl":"https://doi.org/10.5051/jpis.2602840142","url":null,"abstract":"<p><strong>Purpose: </strong>Smoking adversely affects the outcomes of periodontal therapy; however, no studies have evaluated whether smoking cessation is associated with improved outcomes after periodontal regenerative therapy. This retrospective observational study with a quasi-analytical design used propensity score (PS) matching to compare postoperative outcomes between current and former smokers and to evaluate the association between smoking cessation and the outcomes of periodontal regenerative therapy.</p><p><strong>Methods: </strong>Data were retrospectively collected for all sites treated with periodontal regenerative therapy at Nihon University School of Dentistry at Matsudo Hospital between February 2017 and June 2024. Clinical parameters, radiographic assessments, and smoking histories were extracted from electronic medical records. After PS matching, outcomes were compared between current and former smokers. The primary outcome was radiographic bone fill (RBF), and the secondary outcomes were clinical attachment level (CAL) gain and probing depth (PD) reduction at 12 months postoperatively.</p><p><strong>Results: </strong>Before PS matching, 30 sites in current smokers and 25 sites in former smokers were analyzed. After matching, 15 sites from each group were included. Mean RBF was 34.4%±20.0% in current smokers and 56.5%±27.6% in former smokers. Mean CAL gain and PD reduction were 2.07±1.39 mm and 2.67±1.76 mm, respectively, in current smokers and 3.07±1.39 mm and 2.87±1.46 mm, respectively, in former smokers. In a forced-entry multivariable regression analysis, former-smoker status was associated with greater RBF at 12 months postoperatively (regression coefficient, 23.5; 95% confidence interval, 4.15-42.7). CAL gain and PD reduction did not differ significantly between current and former smokers.</p><p><strong>Conclusions: </strong>Although the retrospective study design required comparison of different individuals, smoking cessation was associated with 23.5 percentage points greater RBF at 12 months after periodontal regenerative therapy.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149138938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of biomechanical anchorage between apatite- and calcium titanate-modified implant surfaces.","authors":"Hee-Seung Han, Sungtae Kim, Narasaem Lee, Yongjoon Kim, Kyung-Il Kim, Young-Dan Cho","doi":"10.5051/jpis.2601500075","DOIUrl":"https://doi.org/10.5051/jpis.2601500075","url":null,"abstract":"<p><strong>Purpose: </strong>Implant surface chemistry and microstructure can substantially influence early osseointegration even when overall roughness is comparable. This study compared the surface characteristics, <i>in vitro</i> ion release, and <i>in vivo</i> osseointegration of a calcium titanate (CaTiO₃) nanofilm formed on a sandblasting combined with acid etching (SLA) surface (C surface) and an SLA surface bearing an additional ultrathin apatite nanolayer (A surface).</p><p><strong>Methods: </strong>Implant surface morphology and roughness were evaluated using field-emission scanning electron microscopy, laser confocal microscopy, and contact profilometry. Calcium ion release was quantified using the Arsenazo III colorimetric assay. Eight male mongrel dogs underwent extraction of the mandibular premolars and first molars. After a 3-month healing period, C- or A-surface implants were placed using standardized drilling protocols, and insertion torque was recorded. At 3 and 6 weeks, removal torque (RT) was measured, and histomorphometric analysis was performed to calculate the proportion of the implant surface in direct contact with newly formed bone.</p><p><strong>Results: </strong>Both surfaces exhibited moderately rough micro- and nanostructured topographies with similar roughness parameters. However, calcium ion release was significantly greater from the A surface. RT was also significantly higher for the A surface than for the C surface at both 3 and 6 weeks.</p><p><strong>Conclusions: </strong>Despite similar macrodesign and overall roughness, an ultrathin apatite nanolayer can accelerate early interfacial strengthening without altering midterm osseointegration.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149138917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immediate implant placement using a digital surgical guide with hydroxyapatite nano-coated versus uncoated implants: 1-year results of a single-blind randomized controlled trial.","authors":"Sungrak Ahn, Young-Chang Ko, Jungwon Lee, Dongseob Lee, Yong-Moo Lee","doi":"10.5051/jpis.2600520026","DOIUrl":"https://doi.org/10.5051/jpis.2600520026","url":null,"abstract":"<p><strong>Purpose: </strong>The primary purpose of this study was to compare 1-year implant success rates between hydroxyapatite (HA) nano-coated implants and uncoated sandblasted, large-grit, acid-etched (SLA) implants placed with a digital surgical guide in the posterior maxilla. Secondary aims were to evaluate peri-implant clinical parameters, placement accuracy, emergence angle, and the influence of abutment height.</p><p><strong>Methods: </strong>Twenty-four implants in 19 patients in the HA nano-coated group and 26 implants in 18 patients in the uncoated group were placed flaplessly using digital surgical guides. Outcomes included implant success, probing depth, mucosal recession, keratinized mucosa, vestibular depth, modified plaque index, modified sulcular bleeding index, marginal bone levels (MBLs), implant placement accuracy, and abutment height. Linear mixed-effects models included guide type and implant surface as fixed effects and patient as a random intercept; guide type was categorized as tooth-supported or free-end extension. Regression analyses were used to assess associations between abutment height and emergence angle.</p><p><strong>Results: </strong>At 1 year, implant success did not differ significantly between groups. Clinical indices and MBLs remained stable. Most emergence angles were <30°. In unadjusted comparisons, several deviations from the planned implant position were smaller in the HA nano-coated group. However, in mixed-effects models, guide type was the principal determinant of deviation, with tooth-supported guides showing smaller deviations than free-end extension guides. Abutment height was negatively correlated with emergence angle, and an abutment height of approximately 2 mm was associated with an emergence angle of approximately 30°.</p><p><strong>Conclusions: </strong>At 1 year, HA nano-coated and uncoated SLA implants showed comparable clinical and radiographic outcomes after immediate placement in the posterior maxilla. HA nano-coating provided no measurable benefit, possibly due to a ceiling effect of contemporary SLA surfaces. Digital guide to ensure sufficient abutment height and implant depth may help maintain emergence angles ≤30° and support peri-implant tissue health.</p><p><strong>Trial registration: </strong>Clinical Research Information Service (CRIS) Identifier: KCT0006364.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149138873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic periodontitis induces cognitive decline in young adult mice.","authors":"Kavitha Sajukumar, Prabhakar Yadav, Sang-Joun Yu, Gum Hwa Lee","doi":"10.5051/jpis.2504440222","DOIUrl":"https://doi.org/10.5051/jpis.2504440222","url":null,"abstract":"<p><strong>Purpose: </strong>This study investigated whether chronic periodontitis induces cognitive impairment in young adult mice and examined the mechanisms underlying periodontitis-associated cognitive decline.</p><p><strong>Methods: </strong>Chronic periodontitis was induced in 4-month-old female C57BL/6N mice by bilateral ligature placement around the maxillary molars. Learning and memory were assessed using the Morris water maze (MWM) and novel object recognition tests at 4 and 8 weeks after ligation. At 2 and 12 weeks post-ligation, tissues from the brain, including the hippocampus and prefrontal cortex, as well as the trigeminal ganglion, gingiva, palate, and maxilla, were harvested. Alveolar bone loss and periodontal tissue destruction were evaluated using micro-computed tomography and histology, respectively. Neuroinflammatory markers, including glial fibrillary acidic protein (GFAP), tumor necrosis factor-α, interleukin (IL)-6, and IL-1β, and synapse-related gene expression, including glutamate ionotropic AMPA-type subunit 2 (<i>Gria2</i>), glutamate ionotropic NMDA-type subunit 2A (<i>Grin2a</i>), and semaphorin 4D (<i>Sema4d</i>), were quantified using immunofluorescence and reverse transcription quantitative polymerase chain reaction.</p><p><strong>Results: </strong>Ligature placement resulted in significant alveolar bone loss and periodontal tissue destruction. Early neuroinflammatory changes were evident, including increased expression levels of GFAP in the molecular layer of the dentate gyrus, GFAP in the hippocampus, and IL-6 in the trigeminal ganglion. Behavioral assessments revealed hippocampus-dependent cognitive impairment: at 4 weeks, spatial memory deficits were observed in the MWM; at 8 weeks, increased anxiety-like behavior, impaired recognition memory, and delayed spatial learning were observed. Gene expression analysis demonstrated dysregulation of synapse-related genes, including increased IL-1β and decreased <i>Gria2</i> expression in the prefrontal cortex and reduced <i>Gria2</i>, <i>Grin2a</i>, and <i>Sema4d</i> expression in the hippocampus.</p><p><strong>Conclusions: </strong>Ligature-induced chronic periodontitis in young adult mice caused alveolar bone loss and was associated with neuroinflammation, cognitive deficits, and dysregulated synaptic gene expression. These findings suggest that periodontal disease may contribute to cognitive decline through neuroinflammatory and synaptic mechanisms in younger individuals.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149138890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"State-of-the-art deep learning for dental implant detection: a standardized benchmark for clinical practice.","authors":"Jun-Seok Kang, Yeon-Tae Kim, Akhilanand Chaurasia, Jae-Hong Lee","doi":"10.5051/jpis.2601900095","DOIUrl":"https://doi.org/10.5051/jpis.2601900095","url":null,"abstract":"<p><strong>Purpose: </strong>Although deep-learning approaches show promise for detecting dental implants, the absence of standardized benchmarks has hindered evidence-based model selection. This study systematically compared five contemporary architectures on the same dataset under controlled conditions. A three-seed training-stability analysis was also performed to characterize between-run variability.</p><p><strong>Methods: </strong>YOLOv8-Large, YOLOv11-Large, real-time detection transformer (RT-DETR)-Large, lightweight detection transformer (LW-DETR)-Large, and Faster R-CNN ResNet50-FPN were evaluated using 3,996 annotated panoramic radiographs divided into training, validation, and test sets at an 80:10:10 ratio. Each model was trained using three random seeds to assess training stability. Detection accuracy, measured using the F1 score and mean average precision (mAP), end-to-end pipeline speed, measured using frames per second and latency, and graphics processing unit memory use were evaluated. Pairwise statistical comparisons were performed using the independent-samples <i>t</i>-test with Bonferroni correction. To ensure strict comparability among architectures, all mAP values were calculated using a single unified evaluator, pycocotools COCOeval, applied identically to every model.</p><p><strong>Results: </strong>YOLOv11-Large achieved the highest mAP@0.5 (0.9873±0.0004) and the fastest end-to-end processing speed (87.2 frames per second), with an F1 score of 0.9853±0.0010. YOLOv8-Large performed similarly, with an mAP@0.5 of 0.9859±0.0005 and an F1 score of 0.9868±0.0002; the 95% confidence intervals for mAP@0.5 overlapped between the two models. RT-DETR-Large had the third-highest accuracy (mAP@0.5 = 0.9806±0.0055). LW-DETR-Large achieved an mAP@0.5 of 0.9699±0.0025 but had substantially slower inference (7.1 frames per second), attributable to quadratic attention scaling at a resolution of 1,536×1,536 pixels. Faster R-CNN ResNet50-FPN had lower accuracy (mAP@0.5 = 0.9214±0.0051) and processing speed (14.2 frames per second). All four contemporary detectors significantly outperformed Faster R-CNN ResNet50-FPN (Bonferroni-corrected <i>P</i><0.005).</p><p><strong>Conclusions: </strong>Within this single-center benchmark based on panoramic radiographs, YOLOv11-Large provided the best balance between processing speed and mAP on workstation-class hardware. LW-DETR-Large achieved competitive accuracy but was constrained by Vision Transformer attention scaling at the clinical image resolution used in this study. These findings may inform model selection for dental implant detection on panoramic radiographs. External multicenter and cross-modality validation is required before broader claims regarding clinical deployment can be made.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149138913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of the CXCL12/CXCR4 axis in periodontitis: bioinformatic and clinical evidence.","authors":"Jie Mei, Renjie Shuai, Xiaosong Feng, Kaiwen Nan, Changlin Tang, Fu Jin, Jun Duan","doi":"10.5051/jpis.2600180009","DOIUrl":"https://doi.org/10.5051/jpis.2600180009","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to identify key genes associated with periodontitis using bioinformatic approaches and to validate the expression of the candidate molecules CXCL12 and CXCR4 in gingival tissue samples from patients with periodontitis.</p><p><strong>Methods: </strong>Two large periodontitis-related gene-expression datasets, GSE16134 and GSE10334, were retrieved from the Gene Expression Omnibus database. The datasets were processed, integrated, and screened to identify genes associated with periodontitis. The 10 genes with the highest node degrees were designated hub genes. Least absolute shrinkage and selection operator (LASSO) regression and support vector machine-recursive feature elimination (SVM-RFE) were then used to select signature genes, followed by correlation analysis. Gingival tissues discarded during periodontal surgery were collected from patients with stage III-IV chronic periodontitis. Morphological changes were evaluated using hematoxylin and eosin (H&E) staining, and CXCL12 and CXCR4 protein expression was assessed using immunohistochemistry (IHC). Quantitative polymerase chain reaction (qPCR) was performed to measure <i>CXCL12</i> and <i>CXCR4</i> mRNA expression.</p><p><strong>Results: </strong>Bioinformatic analysis identified <i>CXCL12</i> and <i>CXCR4</i> as the leading signature genes. <i>CXCL12</i> ranked first in both the LASSO and SVM-RFE analyses, and <i>CXCR4</i> showed the strongest correlation with CXCL12 among the hub genes. H&E staining demonstrated markedly greater inflammatory-cell infiltration in the gingival lamina propria of the periodontitis group than in the control group. IHC and qPCR confirmed that CXCL12 and CXCR4 protein and mRNA levels, respectively, were significantly higher in the periodontitis group than in the control group (<i>P</i><0.05).</p><p><strong>Conclusions: </strong>Upregulation of CXCL12 and CXCR4 was significantly associated with periodontitis. These molecules may participate in the periodontal inflammatory response, although their causal and mechanistic roles require further functional investigation.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149138904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between apical periodontitis and retrograde peri-implantitis: a 10-year retrospective study.","authors":"Weiyao Xia, Hao Xu, Yi Man, Yili Qu, Jing Wang","doi":"10.5051/jpis.2501480074","DOIUrl":"https://doi.org/10.5051/jpis.2501480074","url":null,"abstract":"<p><strong>Purpose: </strong>Retrograde peri-implantitis (RPI) has been hypothesized to be associated with apical periodontitis (AP). This retrospective study evaluated whether adjacent AP was associated with RPI and whether implant-to-lesion distance modified this association.</p><p><strong>Methods: </strong>A total of 1,047 patients with 1,819 implants were included; 56 implants in 56 patients were placed near teeth with AP. RPI was diagnosed during follow-up using radiographic and clinical examinations. Firth's penalized logistic regression was first used to assess the association between adjacent AP and RPI development. Implant-to-lesion distance was then analyzed as a continuous variable using Firth's penalized logistic regression and restricted cubic splines to determine whether RPI risk increased as distance decreased. Based on the linear relationship, previous literature, and clinical feasibility, distance was subsequently dichotomized at 3 mm (<3 mm vs. ≥3 mm). Potential risk factors for RPI, including this distance category and other covariates, were examined using a multivariable Firth model. The manifestations and management of RPI were also summarized.</p><p><strong>Results: </strong>The incidence of RPI was 10.71% among implants placed adjacent to teeth with AP (odds ratio [OR], 26.51, <i>P</i><0.01). In the continuous-distance model, shorter implant-to-lesion distance was associated with a higher risk of RPI (β=0.541; OR per 1-mm change, 0.58; 95% confidence interval, 0.25-0.96; <i>P</i>=0.030). Using the statistically and clinically meaningful 3-mm threshold, an implant-to-lesion distance <3 mm was significantly associated with RPI occurrence (OR, 6.48; <i>P</i>=0.03). Five of the 6 AP-related RPI cases received relevant treatment and showed an 83% survival rate at the last follow-up.</p><p><strong>Conclusions: </strong>Within the limitations of this retrospective study, AP in adjacent teeth was associated with RPI. Maintaining a clearance of at least 3 mm from adjacent AP may help reduce the risk of RPI.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149138855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From pages to podiums: launching the <i>JPIS</i> Evidence-Based International Congress and navigating the future of periodontology.","authors":"Jong-Hyuk Chung","doi":"10.5051/jpis.265604edi01","DOIUrl":"10.5051/jpis.265604edi01","url":null,"abstract":"","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":"56 4","pages":"267-268"},"PeriodicalIF":3.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13547811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148876120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"circMYO9B affects the progression of periodontitis by targeting the miR-4731-5p/USP15 axis.","authors":"Huimin Xie, Huiling Qin, Zhengyi Chu, Zhenhua Dai","doi":"10.5051/jpis.2504260213","DOIUrl":"10.5051/jpis.2504260213","url":null,"abstract":"<p><strong>Purpose: </strong>Periodontitis is a common oral disease characterized by inflammation and destruction of periodontal tissues. Competitive endogenous RNAs (ceRNAs) are known to regulate inflammatory responses. This study aimed to investigate the role of circular RNA MYO9B (circMYO9B), as a potential ceRNA, in the progression of periodontitis and to elucidate its underlying regulatory mechanism.</p><p><strong>Methods: </strong>This study included 69 healthy controls and 71 patients with periodontitis. Lipopolysaccharide (LPS)-treated periodontal ligament cells (PDLCs) were used as an <i>in vitro</i> model of periodontitis. Reverse transcription-quantitative polymerase chain reaction was performed to quantify circMYO9B, miR-4731-5p, and ubiquitin-specific peptidase 15 (USP15) expression, while enzyme-linked immunosorbent assays were used to measure inflammatory cytokine levels. Cell proliferation, migration, and apoptosis were assessed using the Cell Counting Kit-8 assay, Transwell migration assay, and flow cytometry, respectively. Dual-luciferase reporter assays were conducted to validate the interactions among circMYO9B, miR-4731-5p, and USP15.</p><p><strong>Results: </strong>circMYO9B expression was significantly elevated in the gingival crevicular fluid of patients with periodontitis and in LPS-treated PDLCs. Receiver operating characteristic curve analysis demonstrated that circMYO9B had diagnostic potential for periodontitis (area under the curve: 0.919). LPS stimulation reduced PDLC viability and migratory capacity while increasing inflammatory cytokine production and apoptosis. circMYO9B directly targeted and negatively regulated miR-4731-5p. Silencing circMYO9B significantly attenuated LPS-induced PDLC dysfunction, whereas inhibition of miR-4731-5p abolished the protective effects of circMYO9B knockdown. USP15 was identified as a direct downstream target of miR-4731-5p: overexpression of miR-4731-5p alleviated LPS-induced cellular injury in PDLCs, but this protective effect was markedly reversed by concomitant USP15 overexpression.</p><p><strong>Conclusions: </strong>circMYO9B is associated with periodontitis progression through the miR-4731-5p/USP15 regulatory axis, suggesting its potential utility as a therapeutic target in periodontitis.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":" ","pages":"308-323"},"PeriodicalIF":3.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13547819/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148764898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}