Hee-Seung Han, Hye-Rim Shin, Sungtae Kim, Young-Dan Cho
{"title":"多核苷酸与交联透明质酸减少炎症和增加胶原合成。","authors":"Hee-Seung Han, Hye-Rim Shin, Sungtae Kim, Young-Dan Cho","doi":"10.5051/jpis.2402520126","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>Gingival fibroblasts are a major cellular component of the connective tissue and play a crucial role in wound healing and tissue regeneration through collagen synthesis. This study aimed to assess the effect of polynucleotide (PN) combined with cross-linked hyaluronic acid (HA) on the proliferation, differentiation, and anti-inflammatory responses of human gingival fibroblasts-1 (HGF-1).</p><p><strong>Methods: </strong>In this <i>in vitro</i> experimental study, HGF-1 cells were cultured with PN/HA, PN, HA, and enamel matrix derivative (EMD). Cell proliferation and migration were assessed at 24 hours and 48 hours, respectively. Long-term cultures were evaluated for collagen and proteoglycan deposition using sirius red and alcian blue staining. Levels of phospho-nuclear factor-κB (NF-κB) p65, collagen type I (Col 1), and interleukin-6 (IL-6) were measured using Western blot analysis or enzyme-linked immunosorbent assay.</p><p><strong>Results: </strong>All experimental groups exhibited increased cellular proliferation and migration compared to the control group. Similar to EMD, PN/HA promoted the synthesis of Col 1 and proteoglycans. At concentrations of 2.5 mg/mL for PN and HA, and 1.0 mg/mL for PN/HA, significant anti-inflammatory effects were observed. These effects included reductions in phospho-NF-κB p65 levels and IL-6 production when exposed to lipopolysaccharides from <i>Porphyromonas gingivalis</i>. EMD, however, did not demonstrate these anti-inflammatory effects.</p><p><strong>Conclusions: </strong>Within the limitations of this study, we concluded that PN/HA improves gingival tissue healing by promoting cell proliferation and migration, facilitating rapid collagen deposition, and exerting anti-inflammatory effects.</p>","PeriodicalId":48795,"journal":{"name":"Journal of Periodontal and Implant Science","volume":" ","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Polynucleotide with cross-linked hyaluronic acid reduces inflammation and increases collagen synthesis.\",\"authors\":\"Hee-Seung Han, Hye-Rim Shin, Sungtae Kim, Young-Dan Cho\",\"doi\":\"10.5051/jpis.2402520126\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>Gingival fibroblasts are a major cellular component of the connective tissue and play a crucial role in wound healing and tissue regeneration through collagen synthesis. This study aimed to assess the effect of polynucleotide (PN) combined with cross-linked hyaluronic acid (HA) on the proliferation, differentiation, and anti-inflammatory responses of human gingival fibroblasts-1 (HGF-1).</p><p><strong>Methods: </strong>In this <i>in vitro</i> experimental study, HGF-1 cells were cultured with PN/HA, PN, HA, and enamel matrix derivative (EMD). Cell proliferation and migration were assessed at 24 hours and 48 hours, respectively. Long-term cultures were evaluated for collagen and proteoglycan deposition using sirius red and alcian blue staining. Levels of phospho-nuclear factor-κB (NF-κB) p65, collagen type I (Col 1), and interleukin-6 (IL-6) were measured using Western blot analysis or enzyme-linked immunosorbent assay.</p><p><strong>Results: </strong>All experimental groups exhibited increased cellular proliferation and migration compared to the control group. Similar to EMD, PN/HA promoted the synthesis of Col 1 and proteoglycans. At concentrations of 2.5 mg/mL for PN and HA, and 1.0 mg/mL for PN/HA, significant anti-inflammatory effects were observed. These effects included reductions in phospho-NF-κB p65 levels and IL-6 production when exposed to lipopolysaccharides from <i>Porphyromonas gingivalis</i>. EMD, however, did not demonstrate these anti-inflammatory effects.</p><p><strong>Conclusions: </strong>Within the limitations of this study, we concluded that PN/HA improves gingival tissue healing by promoting cell proliferation and migration, facilitating rapid collagen deposition, and exerting anti-inflammatory effects.</p>\",\"PeriodicalId\":48795,\"journal\":{\"name\":\"Journal of Periodontal and Implant Science\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-01-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Periodontal and Implant Science\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.5051/jpis.2402520126\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Periodontal and Implant Science","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.5051/jpis.2402520126","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Polynucleotide with cross-linked hyaluronic acid reduces inflammation and increases collagen synthesis.
Purpose: Gingival fibroblasts are a major cellular component of the connective tissue and play a crucial role in wound healing and tissue regeneration through collagen synthesis. This study aimed to assess the effect of polynucleotide (PN) combined with cross-linked hyaluronic acid (HA) on the proliferation, differentiation, and anti-inflammatory responses of human gingival fibroblasts-1 (HGF-1).
Methods: In this in vitro experimental study, HGF-1 cells were cultured with PN/HA, PN, HA, and enamel matrix derivative (EMD). Cell proliferation and migration were assessed at 24 hours and 48 hours, respectively. Long-term cultures were evaluated for collagen and proteoglycan deposition using sirius red and alcian blue staining. Levels of phospho-nuclear factor-κB (NF-κB) p65, collagen type I (Col 1), and interleukin-6 (IL-6) were measured using Western blot analysis or enzyme-linked immunosorbent assay.
Results: All experimental groups exhibited increased cellular proliferation and migration compared to the control group. Similar to EMD, PN/HA promoted the synthesis of Col 1 and proteoglycans. At concentrations of 2.5 mg/mL for PN and HA, and 1.0 mg/mL for PN/HA, significant anti-inflammatory effects were observed. These effects included reductions in phospho-NF-κB p65 levels and IL-6 production when exposed to lipopolysaccharides from Porphyromonas gingivalis. EMD, however, did not demonstrate these anti-inflammatory effects.
Conclusions: Within the limitations of this study, we concluded that PN/HA improves gingival tissue healing by promoting cell proliferation and migration, facilitating rapid collagen deposition, and exerting anti-inflammatory effects.
期刊介绍:
Journal of Periodontal & Implant Science (JPIS) is a peer-reviewed and open-access journal providing up-to-date information relevant to professionalism of periodontology and dental implantology. JPIS is dedicated to global and extensive publication which includes evidence-based original articles, and fundamental reviews in order to cover a variety of interests in the field of periodontal as well as implant science.