多核苷酸与交联透明质酸减少炎症和增加胶原合成。

IF 2.2 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE
Hee-Seung Han, Hye-Rim Shin, Sungtae Kim, Young-Dan Cho
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引用次数: 0

摘要

目的:牙龈成纤维细胞是结缔组织的主要细胞成分,通过胶原合成在伤口愈合和组织再生中发挥重要作用。本研究旨在评估多核苷酸(PN)联合交联透明质酸(HA)对人牙龈成纤维细胞-1 (HGF-1)增殖、分化和抗炎反应的影响。方法:体外实验研究HGF-1细胞分别用PN/HA、PN、HA和搪瓷基质衍生物(EMD)培养。分别在24小时和48小时评估细胞增殖和迁移。用天狼星红和阿利新蓝染色评估长期培养的胶原蛋白和蛋白多糖沉积。采用Western blot或酶联免疫吸附法检测磷酸化核因子-κB (NF-κB) p65、ⅰ型胶原(Col 1)和白细胞介素-6 (IL-6)水平。结果:与对照组相比,所有实验组的细胞增殖和迁移均有所增加。与EMD类似,PN/HA促进了Col 1和蛋白聚糖的合成。在PN和HA浓度为2.5 mg/mL和PN/HA浓度为1.0 mg/mL时,均有显著的抗炎作用。这些影响包括暴露于牙龈卟啉单胞菌脂多糖时磷酸化nf -κB p65水平和IL-6产生的降低。然而,EMD没有表现出这些抗炎作用。结论:在本研究的局限性内,我们得出PN/HA通过促进细胞增殖和迁移、促进胶原快速沉积和发挥抗炎作用来促进牙龈组织愈合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Polynucleotide with cross-linked hyaluronic acid reduces inflammation and increases collagen synthesis.

Purpose: Gingival fibroblasts are a major cellular component of the connective tissue and play a crucial role in wound healing and tissue regeneration through collagen synthesis. This study aimed to assess the effect of polynucleotide (PN) combined with cross-linked hyaluronic acid (HA) on the proliferation, differentiation, and anti-inflammatory responses of human gingival fibroblasts-1 (HGF-1).

Methods: In this in vitro experimental study, HGF-1 cells were cultured with PN/HA, PN, HA, and enamel matrix derivative (EMD). Cell proliferation and migration were assessed at 24 hours and 48 hours, respectively. Long-term cultures were evaluated for collagen and proteoglycan deposition using sirius red and alcian blue staining. Levels of phospho-nuclear factor-κB (NF-κB) p65, collagen type I (Col 1), and interleukin-6 (IL-6) were measured using Western blot analysis or enzyme-linked immunosorbent assay.

Results: All experimental groups exhibited increased cellular proliferation and migration compared to the control group. Similar to EMD, PN/HA promoted the synthesis of Col 1 and proteoglycans. At concentrations of 2.5 mg/mL for PN and HA, and 1.0 mg/mL for PN/HA, significant anti-inflammatory effects were observed. These effects included reductions in phospho-NF-κB p65 levels and IL-6 production when exposed to lipopolysaccharides from Porphyromonas gingivalis. EMD, however, did not demonstrate these anti-inflammatory effects.

Conclusions: Within the limitations of this study, we concluded that PN/HA improves gingival tissue healing by promoting cell proliferation and migration, facilitating rapid collagen deposition, and exerting anti-inflammatory effects.

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来源期刊
Journal of Periodontal and Implant Science
Journal of Periodontal and Implant Science DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
3.30
自引率
5.30%
发文量
38
期刊介绍: Journal of Periodontal & Implant Science (JPIS) is a peer-reviewed and open-access journal providing up-to-date information relevant to professionalism of periodontology and dental implantology. JPIS is dedicated to global and extensive publication which includes evidence-based original articles, and fundamental reviews in order to cover a variety of interests in the field of periodontal as well as implant science.
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