Molecular & Cellular Biomechanics最新文献

{"title":"Finite Element Analysis of Fatigue Behavior of Stent in Tapered Arteries","authors":"Xiang Shen, Hongfei Zhu, Ji Song, Jiabao Jiang, Deng Yongquan","doi":"10.32604/MCB.2019.05737","DOIUrl":"https://doi.org/10.32604/MCB.2019.05737","url":null,"abstract":"In order to open up the blocked lumen and remodel the blood environment, vascular stents were usually used to transplant into narrowed blood vessels. Due to its minimally invasive and highly efficiency, stenting has achieved great success in the treatment of cardiovascular diseases. However, failure of stents due to its fatigue will damage the arterial wall, leading to adverse reactions such as thrombosis and in-stent restenosis (ISR), which severely limited its long-term outcome. Therefore, it was very important to predict the service life of stents, especially in tapered arteries. \u0000FEA was adopted to study the effects of arterial tapering and stent material on the fatigue life of stents. Balloon-stent-plaque-vessel coupling systems were established to simulate the working environment of stents in vivo. Five different tapered vessel models were established to study the vessel tapering level on the fatigue life of stents. Besides, the fatigue life of 316L stainless steel stent and L605 cobalt-chromium (Co-Cr) alloy stent deployed into a 0.43° tapered vessel were analyzed and compared. The Goodman diagram method was adopted to evaluate the fatigue resistance of stents. \u0000Results showed that the stress concentration was found on the inner crown of the strut when the stent was subjected to pulsating blood pressure. It was similar to the stress distribution on the stent after expansion. This also indicated that the crown of the strut played a decisive role in the long-term efficacy of the stent. The maximum average stress of Co-Cr alloy stent was higher than 316L stainless stent. However, the fatigue resistance of stents was improved by simply changing stent material from 316L stainless to L605 cobalt-chromium alloy. So the L605 Co-Cr alloy stent can withstand greater stress without fatigue. In addition, the tapering of the vessel will also affect the fatigue performance of the stent. The stent implantation in tapered vessels could lead to greater residual stress and shorter fatigue life of the stent. With the tapering level gradually increased, the minimum fatigue safety factor of the stent decreased, which indicated the stent was more likely to fatigue. Compared to a straight vessel, the fatigue life of the stent was shortened by 9.8%, when it was deployed in a 1.13° tapered vessel. \u0000The obtained results showed that finite element analysis was an effective tool to predict stent fatigue life. The method that predicted stent fatigue life in tapered vessels can help clinicians select stents that are more suitable for tapered vessels and help stent engineers design stents that are more resistant to fatigue.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77803589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Some Aspects in Mechano-Biology of Platelet and Leukocyte in Blood Flows","authors":"Ying Fang, Jianhua Wu","doi":"10.32604/MCB.2019.05695","DOIUrl":"https://doi.org/10.32604/MCB.2019.05695","url":null,"abstract":"For hemostasis and thrombosis, some proteins, such as Von Willebrand Factor (VWF, a multimeric plasma glycoprotein synthesized in endothelial cells and megakaryocytes and secreted to circulation or attached to endothelial cells), the metalloprotease ADAMTS13 (a disintegrin and metalloprotease with a thrombospondin type 1 motif, member 13), P-selectin (one of three selectin family members with a N-terminal C-type lectin domain, an epidermal growth factor (EGF)-like module, a series of consensus repeat (CR) units, a transmembrane segment and a short cyto-plasmic domain) and β2 integrin. In adhesion and aggregation of circulating platelets towards to the sites of vascular injury, VWF on vascular wall captures and activates the circulating platelets through interaction with platelet receptor glycoprotein Ibα (GPIbα). The activated platelets will secrete P-selectin, mediating flowing leukocytes to be captured first to and then rolled on the platelets. Activation of β2 integrin on leukocytes makes the rolling cells slow down and adhere firmly to platelet. Pathological hemodynamic environment may cause platelet-induced inflammation overreaction of leukocytes, leading to mechanical instability of thrombotic plaque. Above mentioned events all are referred to their respective un-well known mechano-chemistry processes. For better understanding on the mechano-chemistry mechanism of interaction of leukocyte with platelet under flows, we have investigated the force-dependent Structure-function of VWF-A domain, force-regulated cleavage of A2 domain of von Willebrand factor (vWF) by ADAMTS13, P-selectin secretion from activated platelet, and P-selectin-mediated Activation of β2 integrin on leukocytes under shear stresses, and so on through AFM and flow chamber experiments and molecular dynamics simulation. Our data showed that, these events mentioned above were biphasic force-dependent. Increasing force stabilizes the globular VWF-A conformation first and then makes it become a spread one with higher affinity with platelet receptor glycoprotein Ibα (GPIbα), the force-regulated cleavage of VWF-A2 domain by ADAMTS13 maybe closely related to the induced-fit of ADMATS13 and VWF-A2, Force triggers and quickens P-selectin-induced Activation of β2 integrin on leukocytes.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73068386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Coronary Atherosclerotic Plaque Composition Based on Convolutional Neural Network (CNN)","authors":"Yifan Yin, Chunliu He, Biao Xu, Zhiyong Li","doi":"10.32604/MCB.2019.05732","DOIUrl":"https://doi.org/10.32604/MCB.2019.05732","url":null,"abstract":"The tissue composition and morphological structure of atherosclerotic plaques determine its stability or vulnerability. Intravascular optical coherence tomography (IVOCT) has rapidly become the method of choice for assessing the pathology of the coronary arterial wall in vivo due to its superior resolution. However, in clinical practice, the analysis of plaque composition of OCT images mainly relies on the interpretation of images by well-trained experts, which is a time-consuming, labor-intensive procedure and it is also subjective. The purpose of this study is to use the Convolutional neural network (CNN) method to automatically extract the best feature information from the OCT images to characterize the three basic components of atherosclerotic plaque (fibrous, lipid, and calcification). This study selected the OCT images of 20 patients from Nanjing Drum Tower Hospital from 2015.12 to 2016.12. The OCT-reading expert first excluded the images containing the brackets, and then divided all the remaining images, resulting in 1500 plaque OCT images. The expert labeled plaque composition in each image, cutting it into 11*11 image patches and obtained 87390 patches. 75000 of them were set as training examples and the others were set for testing. The classification accuracy of the test set served as the evaluation criterion. The experimental results show that the average classification accuracy of the fibrous, calcification, and lipid patches by the CNN classifier was over 75%, especially to characterize the fibrous patches, whose accuracy could reach more than 80%. The proposed method is effective and robust in the analysis of atherosclerotic plaque composition in coronary OCT images, providing a base for further segmentation study.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80948180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Numerical Investigation of the Hemodynamic Environment Change in Patient-Specific Intracranial Aneurysm with Progressive Stenosis in Unilateral Internal Carotid Artery","authors":"Guangyu Zhu, Yuan Wei, Q. Yuan, Ge Yan, Jian Yang","doi":"10.32604/MCB.2019.05730","DOIUrl":"https://doi.org/10.32604/MCB.2019.05730","url":null,"abstract":"Intracranial aneurysm (IAs) is a frequently localized cerebral vascular disorder of an overall prevalence of 5-8% [Cebral (2013)]. Subarachnoid hemorrhage (SAH) caused by IAs rupture is one of the major causes of mortality and morbidity in the modern world. Local hemodynamic characteristics played important roles in the rupture of IAs and has been studied extensively from different perspectives [Liu (2015); Brinjikji (2017)]. However, the influence of progressive cerebral stenosis on the hemodynamic of the distal cerebral aneurysm is yet to be further investigated. In this study, a set of patient-specific computational fluid dynamics (CFD) simulations were performed to investigate the impact of internal carotid artery (ICA) stenosis growing on the hemodynamic environments in an anterior communicating artery aneurysm (ACoAA).","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80014748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Aortic Flow Patterns in Patients with and without Aortic Valve Disease: Hemodynamic Simulation Based on PC-MRI and CTA Data","authors":"Lijian Xu, Lekang Yin, F. Liang","doi":"10.32604/MCB.2019.05741","DOIUrl":"https://doi.org/10.32604/MCB.2019.05741","url":null,"abstract":"Recent studies have revealed that aortic valve diseases are associated with the increased incidence of the aortopathy development. However, the influence of aortic valve diseases on aortic hemodynamics remains unclear. The purpose of this study was therefore to investigate the hemodynamic differenecs in patients with and without aortic valve disease through patient-specific simulations performed on two aorta models (BAV with severe stenosis vs. normal tricuspid aortic valve (TAV)). Realistic geometries and boundary conditions were obtained from computed tomography angiography (CTA) and phase-contrast magnetic resonance imaging (PC-MRI) measurements, respectively. In addition, 4D-MRI were performed to validate the the numerical methods used to simulate transient flow characteristics. Obtained results shown that the 3D streamlines in the patient with normal TAV were relatively symmetric and evenly distributed. For the patient with BAV, concentrated and high-speed inflow jets were found to impinge on the ascending aorta accompanied by strong vortices. These results indicate that the aortic valve phenotype plays a crucial role in featuring the disturbed flows primarily in ascending aorta, which may relate to the development of aortic diseases.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88781948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-Modality Image-Based Modeling Approach for Cardiovascular Disease: Simulation, Assessment, Prediction, and Virtual Surgery","authors":"D. Tang","doi":"10.32604/mcb.2019.05170","DOIUrl":"https://doi.org/10.32604/mcb.2019.05170","url":null,"abstract":"Medical imaging and image-based modeling have made considerable progress in recent years in cardiovascular research, such as identifying atherosclerotic plaque morphological and mechanical risk factors which may be used in developing improved patient screening strategies, and performing virtual heart surgery seeking optimal surgical procedures for best post-surgical outcome. We will report recent progress in using multi-modality image-based models to predict vulnerable plaque progression and vulnerability change.   In particular, we will report our recent results using IVUS+OCT data to obtain more accurate stress/strain calculations. Inflammation and cap erosion will affect cap material properties. If OCT image could provide inflammation and erosion information, cap stress/strain calculation can be further improved. Preliminary results using molecular imaging will be presented. \u0000Patient-specific ventricle models were constructed to perform virtual surgery for patients with repaired tetralogy of Fallot (TOF) to predict post-surgery outcome, and to seek parameters which could be used to identify patients who could benefit more from pulmonary valve replacement surgery. Cardiac magnetic resonance (CMR) data were obtained from 16 TOF patients (8 male, median age, 42.75) and 6 healthy group (HG) volunteers (1 male, median age, 20.1). CMR-based patient-specific computational RV/LV models were constructed to obtain RV wall thickness (WT), volumes, curvature, and mechanical stress and strain for comparative analysis. It was found that mechanical stress could be a good predictor for surgical outcome. \u0000Acknowledgement: This research was supported in part by NIH grants R01 EB004759, R01 HL089269, NSF-China grants 11672001, 81571691, and a Jiangsu Province Science and Technology Agency grant BE2016785. \u0000Keywords: Cardiovascular disease, ventricle model, vulnerable plaque.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75511910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Influence of Enhanced External Counterpulsation Intervention on the Biomechanical Stress Distribution of Advanced Plaque: A 3D FSI Study Based on in vivo Animal Experiment","authors":"Yahui Zhang, Hui Wang, Zhouming Mai, Jianhang Du, Guifu Wu","doi":"10.32604/MCB.2019.05836","DOIUrl":"https://doi.org/10.32604/MCB.2019.05836","url":null,"abstract":"Enhanced external counterpulsation (EECP) is an effective therapy to provide beneficial assistance for the failing heart by reducing cardiac afterload and increasing blood flow perfusion noninvasively. The technique of EECP involves the use of the EECP device to inflate and deflate a series of compression cuffs wrapped around the patient’s calves, lower thighs, and upper thighs. As the result, the enhanced flow perfusion is derived from the device’s propelling blood from veins of lower body to arteries of upper body and increases the blood supply for the important organs and brain. \u0000In the ACCF/AHA Guideline and ESC Guideline on the management of stable ischemic heart disease and coronary artery disease, EECP has been given a Class IIb and Class IIa recommendation respectively. In the ASA Guideline on the early management of Acute Ischemic stroke, EECP has been given a Class IIa recommendation. \u0000However, considering that EECP performance significantly elevates the acute blood pressure level as well as the perfusion level in systemic arterial tree and in diastolic, whether it will increase the risk of hypertension-related events such as atherosclerotic plaque rupture causes great concern in its clinical applications. But, the influence of EECP intervention on plaque progression remains elusive to this day and need to be assessed. \u0000It is generally agreed that biomechanical stresses play important roles in advanced atherosclerotic plaque progression and rupture. We hypothesized that EECP may intervene the advanced plaque progression via induced the variation of mechanical stresses in plaques. \u0000In this present paper, a pilot study containing animal experiment and numerical simulation was conducted to quantify the variations of biomechanical stresses of the plaque during EECP intervention. An experimentally induced hypercholesterolemic porcine model was developed and the basic hemodynamic measurements were performed in vivo before and during EECP intervention. Meanwhile, 3D multi-component idealized models with fluid-structure interactions (FSI) for carotid atherosclerotic plaques were introduced to solve the wall shear stress (WSS) and plaque structural stress (PWS) before and during intervention. Three cases of fibrous cap thickness (FC=600, 200 and 65μm) and three cases of material properties of the plaque components (normal, hard and soft) were involved in the current study. \u0000Numerical results showed that the max WSS level at the throat of the plaque, the 3D critical plaque wall stress (3D CPWS) level at the fibrous cap and the global max plaque wall stress (GMPWS) level at the shoulder of the plaque were all significantly augmented during EECP. When FC=65μm, EECP intervention augmented 118.1%±1.1% of the Max WSS level, 15.56%±1.68% of the 3D CPWS level and 14.78%±0.24% of the GMPWS level comparing to situations without EECP intervention. \u0000Considering that blood flow shear stress and structural stress are indexes that are presently thought might c","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86642998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Finite Element Analysis for Type B Aortic Dissection Treated with Two Types of Stent Grafts","authors":"Zhuangyuan Meng, T. Ma, Shengzhang Wang, Z. Dong, W. Fu","doi":"10.32604/MCB.2019.05706","DOIUrl":"https://doi.org/10.32604/MCB.2019.05706","url":null,"abstract":"Retrograde type A dissection (RTAD) and stent graft-induced new entry (SINE) is one of the most common post-TEVAR complications, and is defined as a new tear caused by the stent-graft itself (Dong Z.H. et al., 2009). Presumably, the mechanical action and potential damage of stent-graft to the aorta is related to the stent-graft and the anchoring position, mainly from two aspects: (1) The metal skeleton functions to fix the whole support to the wall of the aorta because it is self-inflating and strong in radial force. (2) After implantation, the stent-graft is bent into the arch like a bent spring, with a tendency to be straight. This may result in elastic recoil force to the aortic wall. This study investigated the occurring reasons of new lesions from the biomechanical and mechanobiological view when stent-grafts were implanted into the true lumen to treat an aortic dissection.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82841832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting Plaque Progression Using Patient-Specific Fluid-Structure-Interaction Models Based on IVUS and OCT Images with Follow-Up","authors":"Xiaoya Guo, D. Tang, D. Molony, Chun Yang, H. Samady, Jie Zheng, G. Mintz, A. Maehara, Jian Zhu, G. Ma, M. Matsumura, D. Giddens","doi":"10.32604/MCB.2019.05743","DOIUrl":"https://doi.org/10.32604/MCB.2019.05743","url":null,"abstract":"Atherosclerotic plaque progression is generally considered to be closely associated with morphological and mechanical factors. Plaque morphological information on intravascular ultrasound (IVUS) and optical coherence tomography (OCT) images could complement each other and provide for more accurate plaque morphology. Fluid-structure interaction (FSI) models combining IVUS and OCT were constructed to obtain accurate plaque stress/strain and flow shear stress data for analysis. Accuracy and completeness of imaging and advanced modeling lead to accurate plaque progression predictions. \u0000In vivo IVUS and OCT coronary plaque data at baseline and follow-up were acquired from left circumflex coronary and right coronary artery of one patient with patient’s consent obtained. Co-registration and segmentation of baseline and follow-up IVUS and OCT images were performed by experts. Baseline and follow-up 3D FSI models with cyclic bending based on merged IVUS and OCT data were constructed to obtain plaque stress/strain and flow shear stress data for plaque progression prediction. Nine factors (6 morphological factors and 3 mechanical factors) including average cap thickness, lipid area, calcification area, lumen area, plaque area, plaque burden, wall shear stress (WSS), plaque wall stress (PWS) and plaque wall strain (PWSn) were selected for each slice. Plaque area increase (PAI) and plaque burden increase (PBI) were chosen to measure plaque progression and serve as the target variables for prediction. All possible combinations of nine factors were fed to a generalized linear mixed model for PAI and PBI prediction and quantification of their prediction accuracies. \u0000In this paper, prediction accuracy was defined as the sum of sensitivity and specificity. The optimized predictor combining 9 factors gave the best prediction for PAI with accuracy=1.7087 (sensitivity: 0.8679; specificity: 0.8408). PWSn was the best single-factor predictor for PAI with accuracy=1.5918 (sensitivity: 0.7143; specificity 0.8776). A combination of average cap thickness, calcification area, plaque area, PWS and PWSn gave the best prediction for PBI with accuracy=1.8698 (sensitivity: 0.8892; specificity: 0.9806). PWSn was also the best single-factor predictor for PBI with accuracy=1.8461 (sensitivity: 0.8784; specificity 0.9677). Although WSS was commonly accepted as an important factor for plaque progression, it showed relatively poor ability for prediction of plaque progression in any measure (accuracy, sensitivity, specificity of PAI: 1.0607, 0.0893, 0.9714; PBI: 1.5431/0.6811/0.9032). \u0000Combining morphological and mechanical risk factors may lead to more accurate progression prediction, compared to the predictions using single factor. PWSn is better than WSS for plaque progression using single factor. IVUS+OCT formed basis for accurate data for morphological and mechanical factors. \u0000Acknowledgement: This research was supported in part by NIH grant R01 EB004759, and a Jiangsu Province Sc","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83487136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Short-Term Exposure in PM0.1 on Cardiac Remodeling and Dysfunction in Myocardial Infraction Mice","authors":"Yufan Huang, Pei Niu, Li Li, Y. Huo","doi":"10.32604/MCB.2019.05726","DOIUrl":"https://doi.org/10.32604/MCB.2019.05726","url":null,"abstract":"We aimed to illustrate the association between short-term exposure PM0.1 and heart failure in myocardial infarction (MI) mice. Six-week-old ICR mice were divided into three groups randomly: sham group, MI group and MI exposure group, 12 mice in each group. LAD ligation operation was performed in MI group and MI exposure group. After postoperative two weeks MI exposure mice were put into ventilation chamber which filled with 500ug/m3 PM0.1 for 6 hours per day, while MI group mice and sham group mice were cultivated in normal environment. After exposure 8 weeks, we use Vevo 2100 machine to acquire heart function measurements. Then we collected blood sample and killed mice to obtain heart samples. The proliferation of myocardium were measured by immunofluorescence. Elisa was performed to detect the catecholamine expression in plasma. The changes of collagen were measured by Sirus red stain method. Compared with the sham group, the EF and FS in the MI group were significantly decreased (p<0.05), and MI exposure group showed higher amplitude decrease. The immunofluorescence result showed that the number of proliferating cell in MI exposure group did not change significantly. In addition, the IL-11 in the peripheral blood of MI exposure group did not change significantly, while Sirus red stain showed the content of collagen in MI exposure group increased significantly (p<0.05). In conclusion, short-term exposure in PM0.1 can exacerbate cardiac remodeling and dysfunction, while it had effect neither on IL-11 in peripheral blood nor on myocyte proliferation.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87806235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}