Molecular & Cellular Biomechanics最新文献

{"title":"High Glucose Reduces the Shear Stress-Induced CD59 Expression on EPCs through F-Actin Alteration","authors":"Na Liu, Xiaoyun Zhang, Yuzhen Ding, Hong Li, Xiumei Guan, Min Cheng, Xiaodong Cui","doi":"10.32604/MCB.2019.05751","DOIUrl":"https://doi.org/10.32604/MCB.2019.05751","url":null,"abstract":"Endothelial progenitor cells (EPCs) play a vital role in postnatal vascular injury and repair, especially vasculogenesis and angiogenesis. The purpose of this study was to investigate the effect of laminar shear stress in attenuating the decreased-expression of complement regulatory protein CD59 and the mechanism of cytoskeleton F-actin. Methods: EPCs were isolated from human umbilical vein blood and planted on glass slides, which applied to the laminar shear stress force (12 dyne/cm2) in a high glucose (20 mM) culture environment. The gene and protein expression of CD59 were detected by SYBGreen quantitative PCR and fluorescence activated cell sorter (FACS) respectively. The rearrangement of cytoskeleton F-actin was detected by FITC-phalloidin staining. Results: The elevated effect of shear stress on the expression of CD59 was significantly reduced in high glucose condition. Moreover, we found that F-actin was disorganized by high glucose, while rearrangement of cytoskeleton would be reversed by a moderate concentration of jasplakinolide (JAS) intervention. Conclusion: \u0000Our study indicated that high glucose inhibiting the rearrangement of EPCs cytoskeleton resulted the sensitivity of EPCs to laminar shear stress which should elevate the expression of complement regulatory protein CD59. As a result, EPCs was sensitive to membrane attack complex (MAC) -mediated cell autolysis.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90746144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research and Clinical Applications of Biomechanical Analysis in Optimization of Coronary Interventions","authors":"S. Tu","doi":"10.32604/MCB.2019.05704","DOIUrl":"https://doi.org/10.32604/MCB.2019.05704","url":null,"abstract":"Coronary artery disease (CAD) is the leading cause of mortality and morbidity worldwide. It is the disease of the blood vessels supplying the heart muscle. The fatty plaques built within the walls of the coronary arteries might rupture, creating a thrombus, thereby blocking the entire flow through the vessel, which is followed by a heart attack. Patients who suffer from CAD with documented ischemia are predominately sent to the catheterization laboratory for an invasive procedure (PCI, or percutaneous coronary intervention) to open the vessel by the placement of a “stent” as a scaffolding device to release from ischemia. Identifying the culplit lesions that cause the actual ischemia is crucial for PCI optimization. It has been shown in many clinical trials that the integration of coronary imaging and physiology is better in guiding PCI compared to imaging alone. Over the past years, we have developed approaches to derive coronary physiological data using image reconstruction and biomechanical analysis, thus realizing seamless co-registration between imaging and physiology without using extra invasive devices to measure coronary physiology. Some of these approaches are being transferred into clinical applications that have potential to increase the utility of physiological assessment in patients with CAD. In this talk, I will present these activities and our efforts in developing practical solutions for tailored treatment strategies.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81511112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Intracoronary OCT in Diagnosis and Treatment of Acute Coronary Syndrome","authors":"H. Jia, Bo Yu","doi":"10.32604/MCB.2019.05708","DOIUrl":"https://doi.org/10.32604/MCB.2019.05708","url":null,"abstract":"Coronary angiography is the traditional standard imaging modality for visual evaluation of coronary anatomy and guidance of percutaneous coronary interventions (PCI). However, the 2-dimensional lumenogram cannot depict the arterial vessel per se and plaque characteristics, or directly assess the stenting result. Intracoronary imaging by means of intravascular ultrasound (IVUS) and optical coherence tomography (OCT) provides valuable incremental information that can be used clinically to optimize stent implantation and thereby minimize stent-related problems. Beyond guidance of stent selection and optimisation, imaging provides critical insights into the pathophysiology of acute coronary syndrome (ACS), greater clarity when confronted with angiographically ambiguous lesions and highlights the dynamic nature and significance of atherosclerotic coronary plaque. For several decades, most physicians have believed that ACS is caused by coronary thrombosis resulting from rupture of vulnerable plaque characterized by a thin fibrous cap overlying a large necrotic core and massive inflammatory cell infiltration. However, nearly one-third of ACS cases are caused by plaque erosion characterized by intact fibrous cap, less or absent necrotic core, less inflammation, and large lumen. Because of the limitations of current imaging modalities, including angiography and intravascular ultrasound, the importance of plaque erosion as a cause of acute coronary events is less well known. OCT as an emerging modality with extremely high resolution is the only intravascular imaging modality available for identification of plaque erosion in vivo, which provides new insight into the mechanism of ACS. More importantly, the introduction of OCT to clinical practice enables us to differentiate the patients with ACS caused by plaque erosion from those caused by plaque rupture, thereby providing precise and personalized therapy based on the different underlying mechanisms. This presentation will systematically review the morphological characteristics of plaque erosion identified by OCT and its implications for the management of ACS.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"112 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77713887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ventricle Stress/Strain Comparison Between Models Using Different Zero-Load Diastole and Systole Morphologies and Models Using Only One Zero-Load Morphologies","authors":"Han Yu, P. J. Nido, T. Geva, Chun Yang, Zheyang Wu, R. Rathod, Xueying Huang, K. Billiar, D. Tang","doi":"10.32604/MCB.2019.05837","DOIUrl":"https://doi.org/10.32604/MCB.2019.05837","url":null,"abstract":"Ventricle mechanical stress and strain calculations play an important role in cardiovascular investigations. Patients with repaired tetralogy of Fallot (TOF) account for the majority of cases with late onset right ventricular (RV) failure. The current surgical approach, including pulmonary valve replacement(PVR), has yielded mixed results with some patients recover RV function after pulmonary valve insertion with or without concomitant RV remodeling surgery but some do not[Therrien, Siu and McLaughlin (2000);]. Cardiac magnetic resonance (CMR) data were collected from 6 healthy volunteers and 12 Tetralogy of Fallot (TOF) patients before PVR with consent obtained. 12 patients were divided into two groups depending on right ventricle post-surgery recover( 6 for each group). 3D patient-specific CMR-based ventricle models with different zero-load diastole and systole geometries were constructed to qualify right ventricle (RV) stress and strain values at begin-filling, end-filling, begin-ejection, and end-ejection, respectively. The models are solved with ADINA. Our new models (called 2G models) could provide end-diastole and end-systole stress/strain values which the old models with only one zero-load geometries (called 1G models) could not provide[Tang, Del Nido, Yang, et al., 2016]. Logistic regression with 5-fold cross validation was adopted to predict pulmonary valve replacement outcome.  The results showed 2G mean end-ejection stress value from the 18 participants was 321.4% higher than that from 1G models (p=0.0002). 2G mean strain values was 230% higher than that of 1G models (p=0.0002). TOF group (TG) end-ejection mean stress value was 105.4% higher than that of healthy group (HG) (17.54±7.42kPa vs. 8.54±0.92kPa, p=0.0245).  Worse outcome group (WG) begin-ejection mean stress was 57.4% higher than that of better outcome group (BG, 86.94±26.29 vs. 52.93±22.86 kPa; p=0.041). Among 7 chosen parameters (stress, strain, age, gender, right volume end-diastole volume index, right volume end-systole volume index and ejection fracture), end-filling stress was the best predictor to differentiate BG patients from WG patients with prediction accuracy = 0.8208.  2G models may provide more accurate stress/strain results than 1G models and be applied in clinical situation, potentially.  Large scale studies are still needed for validation.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75962927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neovascularization and Intraplaque Hemorrhage in Atherosclerotic Plaque Destabilization-A Mathematical Model","authors":"Muyi Guo, Y. Cai, Zhiyong Li","doi":"10.32604/MCB.2019.05727","DOIUrl":"https://doi.org/10.32604/MCB.2019.05727","url":null,"abstract":"Observational studies have identified angiogenesis from the adventitial vasa vasorum and intraplaque hemorrhage (IPH) as critical factors in atherosclerotic plaque progression and destabilization. Here we propose a mathematical model incorporating intraplaque neo vascularization and hemodynamic calculation for the quantitative evaluation of atherosclerotic plaque hemorrhage. An angiogenic microvasculature based on histology of a patient’s carotid plaque is generated by two - dimensional nine - point model of endothelial cell migration. Three key cells (endothelial cells, smooth muscle cells and macrophages) and three key chemicals (vascular endothelial growth factors, extracellular matrix and matrix metalloproteinase) are involved in the intraplaque angiogenesis model, and described by the reaction - diffusion partial equations. The hemodynamic calculation of the microcirculation on the generated microvessel network is carried out by coupling the intravascular, interstitial and transvascular flow. The plasma concentration in the interstitial domain is defined as the description of IPH area according to the diffusion and convection with the interstitial fluid flow, as well as the extravascular movement across the leaky vessel wall. The simulation results demonstrate a series of pathophysiological phenomena during the progression of an atherosclerotic plaq ue, including the high microvessel density (MVD) region at the shoulder areas, the transvascular flow through the capillary wall and the intraplaque hemorrhage. The hemodynamic results show significant consistency with both the histology data and the MR im aging data in quality and quantity. In addition, the sensitivity analysis of IPH to model parameters reveals that the decreased MVD and the vessel permeability may reduce the IPH area dramatically.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77639128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Ultrafine Nano-Zinc Particles on Cardiac Structure and Function in Myocardial Infarction Rabbits","authors":"Pei Niu, Li Li, Yufan Huang, Y. Huo","doi":"10.32604/MCB.2019.05724","DOIUrl":"https://doi.org/10.32604/MCB.2019.05724","url":null,"abstract":"Due to ultrafine Nano-zinc particles are major component of PM0.1, so we aimed to  investigate how ultrafine Nano-zinc particles effect on cardiac structure and function in myocardial infarction (MI) rabbits.  We chose twenty-four New Zealand rabbits who were divided into sham group, MI group and MI exposure group randomly, 8 rabbits in each group. We preformed LAD ligation operation in MI group and MI exposure group. After two weeks rabbits suffered from MI successfully, we put the MI exposure group into ventilation chamber filled with 500ug/m3 ultrafine Nano-zinc particles for 6 hours per day. Sham group and MI group were raised in normal environment. Four weeks later, the heart function was detected using vevo2100 machine and all rabbits sacrificed, because we need to collect blood sample and heart tissue. According to echocardiography measurements, we found EF and FS both in MI group and MI exposure group decreased significantly, especially MI exposure group had severe decrease compared with MI group. In addition, left ventricular end-diastolic pressure (LVEDP) in MI exposure group increased obviously and LV +dp/dt max decreased significant. Both the formers in MI group changed to a lesser degree. HE staining results showed myocytes disorganization and LV wall thinning in MI and MI exposure groups. Masson trichrome staining showed that MI exposure group had maximum collagen. In summary, inhalation of ultrafine Nano-zinc particles indeed is harmful for MI rabbits. Moreover, ultrafine Nano-zinc particles can effect on myocardial systolic and diastolic function, thus promote the development from MI to heart failure.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"99 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85279597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Degree of Question Mark of Aorta Can Predict the Thrombosis Rate in the False Lumen of a Type-B Aortic Dissection After TEVAR","authors":"Da Li, Liqing Peng, Yi Wang, D. Yuan, T. Zheng","doi":"10.32604/MCB.2019.05718","DOIUrl":"https://doi.org/10.32604/MCB.2019.05718","url":null,"abstract":"Objective：Thoracic endovascular aortic repair (TEVAR) of type B aortic dissection (AD) is to initiate the thrombosis in the false lumen to eventually result in aortic remodeling. We aim to find out whether the false lumen (FL) thrombosis rate after TEVAR can be predicted accurately by an index that expresses the degree of aortic arch angulation. Method: Three-dimensional aortic arch geometry of 39 type B AD patients (mean age: 48years) after TEVR were reconstructed from post-operative CT images. For the first time, the question mark which take into account the curvature of both aortic arch and the descending aorta was introduced to indicate the aortic arch morphology. The degree of question mark and the angles of aortic arch defined by previous literatures were measured. Moreover, hemodynamics for ADs with different question mark degree were numerically computed using different models. Result: No correlation between the aortic arch angles defined by previous researchers and the rate of thrombosis. However, the degree of question mark has negatively correlation with FL thrombosis (r=-.7394; p<.001). In addition, regions of recirculating flow and low wall shear stress (WSS) in the FLs decreased with the increase of the degree of question mark of the aorta. Conclusion: The degree of question mark proposed in this paper is a good description of the aortic arch geometry after TEVAR in patients with aortic B-type dissection. It is negatively correlated with postoperative FL thrombosis, the higher the question mark degree was, the less likely it was to form a complete thrombus.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"138 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79817382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanical Characterization and Constitutive Modeling of Rabbit Aortas in Health and Diabetes","authors":"Zhi Zhang, J. Tong","doi":"10.32604/MCB.2019.05721","DOIUrl":"https://doi.org/10.32604/MCB.2019.05721","url":null,"abstract":"Diabetes is a major risk factor to cause macrovascular diseases and plays a pivotal role in aortic wall remodeling. However, the effects of diabetes on elastic properties of aortas remain largely unknown. Thirty adult rabbits (1.6-2.2 kg) were collected and the type I diabetic rabbit model was induced by injection of alloxan. A total of 15 control and 15 diabetic rabbit (abdominal) aortas were harvested. Uniaxial and biaxial tensile tests were performed to measure ultimate tensile strength and to characterize biaxial mechanical behaviors of the aortas. A material model was fitted to the biaxial experimental data to obtain constitutive parameters. Histological and mass fraction analyses were performed to investigate the underlying microstructure and dry weight percentages of elastin and collagen in the control and the diabetic aortas. No statistically significant difference was found in ultimate tensile strength between the control and the diabetic aortas. Regarding biaxial mechanical responses, the diabetic aortas exhibited significantly lower extensibility and significantly higher tissue stiffness than the control aortas. Notably, tissue stiffening occurred in both circumferential and axial directions for the diabetic aortas; however, mechanical anisotropy does not change significantly. The material model was able to fit biaxial experimental data very well. Histology showed that a number of isolated foam cells were embedded in the diabetic aortas and hyperplasia of collagen was identified. The dry weight percentages of collagen within the diabetic aortas increased significantly as compared to the control aortas, whereas no significant change was found for that of elastin. The results suggest that the diabetes impairs elastic properties and alters microstructure of the aortas and consequently, these changes may further contribute to complex aortic wall remodeling.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79850413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TET1 Alternative Isoform Regulates Oscillatory Shear Stress Induced Endothelial Dysfunction","authors":"Lu Huang, J. Qiu, Guixue Wang","doi":"10.32604/MCB.2019.05714","DOIUrl":"https://doi.org/10.32604/MCB.2019.05714","url":null,"abstract":"Oscillatory shear stress (OSS) is one of the major risk factors related to endothelial (EC) dysfunction, which contributes to atherosclerosis. Our previous study indicated that inhibitor of DNA binding1 (Id1) play vital role in the regulation of OSS mediated EC function related to atherosclerosis. However, the initiation mechanism during this process remains to be elucidated. Ten-eleven Translocation protein 1 alternative isoform (Tet1s) is a newly reported protein that may have function in adult tissue. Here, we investigate the role of Tet1s in regulating OSS mediated endothelial dysfunction and its underlying mechanism. \u0000    First, physical interaction between Tet1s and Id1 was found and proved by immunoprecipitation. By using carotid partial ligation mice model in vivo and OSS applied on human umbilical venous endothelial cell (HUVEC) in vitro, we found that EC proliferation rate and adhesion molecule expression were upregulated in the local area with OSS characteristics. Compared to the grater curvature (laminar shear stress), a lower Tet1s expression level in atheroprone lesser curvature (OSS) suggested Tet1s regulate the EC function under OSS. This notion is supported by the decline of Tet1s expression in cell culture model. In order to explore the Tet1s expression regulated mechanism, the potential binding sites in Tet1s promoter region for CEBPB was identified by in silico analysis. By using PKA/CEBPB inhibitor H89, we found that H89 inhibited Tet1s expression. HUVEC cell proliferation, proinflammation gene expression as well as monocytes adhesion were enhanced after knockdown of Tet1s  by specific siRNA. And overexpression of Tet1s eliminated OSS induced HUVEC proliferation and inflammation. Further studies revealed Tet1s negatively regulated the expression of Id1. Meanwhile, knockdown of Tet1s induced nucleocytoplasmic shuttling of Id1. \u0000    Our finding indicates a significant role of Tet1s in regulating OSS mediated endothelial dysfunction with respect of abnormal proliferation and inflammation though Id1-dependent pathway.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"106 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88038656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using 3D Thin-Layer Model with in Vivo Patient-Specific Vessel Material Properties to Assesse Carotid Atherosclerotic Plaque Vulnerability","authors":"Qingyu Wang, D. Tang, G. Canton, Zheyang Wu, T. Hatsukami, K. Billiar, C. Yuan","doi":"10.32604/MCB.2019.05748","DOIUrl":"https://doi.org/10.32604/MCB.2019.05748","url":null,"abstract":"Image-based computational models have been introduced to calculate plaque stress/strain conditions and investigate their association with plaque progression and rupture [Tang, Yang, Zheng, et al. (2004)]. However, the accuracy of the computational results is heavily dependent on the data and assumptions used by those models. Patient-specific vessel material properties are in general lacking in image-based computational models, limiting the accuracy of their stress/strain calculations. \u0000A noninvasive approach of combining in vivo 3D multi-contrast and Cine magnetic resonance imaging (MRI) and computational modeling was used to quantify patient-specific carotid plaque material properties for potential plaque model improvements [Wang, Canton, Guo, et al. (2017)]. The stress-based plaque vulnerability index (SPVI) was proposed to combine mechanical analysis, plaque morphology and composition for more complete carotid plaque vulnerability assessment.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"242 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80523629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}