Molecular & Cellular Biomechanics最新文献

{"title":"Co-stimulation effect of fluid shear stress-material surface chemistry on the behavior of human umbilical vein endothelial cells","authors":"Yan Liu, Xiang Zhang, Zheng-Zheng Shi, Lin Zhou, Meng Tong, Lan Cheng","doi":"10.62617/mcb1553","DOIUrl":"https://doi.org/10.62617/mcb1553","url":null,"abstract":"Objective: The improvement of bone repair scaffolds to enhance their bioactivity and in vivo vascularization is a current research hotspot. Method: HUVECs are subjected to both fluid shear stress (FSS) and chemical stimuli simultaneously. The release of ATP, NO, and the expression of eNOS were examined. The adhesion spots and cytoskeleton formed by HUVEC on the material surface were also observed. Result: LFSS (low fluid shear stress, 5 dyn/cm2) did not trigger a response on Glass and -NH2 HUVECs, but induced a strong response on -OH and -CH3, while PFSS (physiological fluid shear stress, 15 dyn/cm2) and HFSS (high fluid shear stress, 20 dyn/cm2) generated responses of all groups of cells, among which the strongest response level was from the -NH2 group, followed by Glass, and among which equal response levels of the -OH and -CH3 groups existed at the lowest. Conclusion: The chemical functional groups changed the initial threshold of HUVECs response to FSS and the shear force stimulation threshold for optimal cellular response by influencing the quality of adhesion spots and cytoskeleton formed by HUVECs on the surface of the material, thereby altering the response state of endothelial cells to shear force stimulation.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"22 5","pages":"1553-1553"},"PeriodicalIF":0.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ojs.sin-chn.com/index.php/mcb/article/download/1553/1114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147921618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro study on the effects of mechanical load on the behavior and signal pathway regulation of pediatric hip cartilage cells","authors":"Yan Liu, Peng Yang, Zhijian Pan, Guangxi Chen, Hongliang Liu, Huang Jh","doi":"10.62617/mcb1240","DOIUrl":"https://doi.org/10.62617/mcb1240","url":null,"abstract":"Objective: To explore the effects of mechanical load on the behavior and related signaling pathways of pediatric hip cartilage cells, providing experimental evidence for optimizing cartilage regeneration and repair strategies. Methods: Human-derived pediatric hip cartilage cells were cultured in vitro and subjected to uniaxial tensile stress at a specific frequency (1 Hz) with varying strain amplitudes (0%, 2%, 5%, 10%). Cell proliferation curves were assessed using the CCK-8 assay, while qPCR was used to detect changes in the expression of COL2A1, ACAN, and genes such as ITGB1, TGF-β1, WNT3A, MAPK1, among others. Western Blot analysis was conducted to measure protein levels of Collagen II, Aggrecan, MMP-13, as well as p-ERK, p-p38, β-catenin, MAPK1, and MAPK14. The relationship between strain amplitude and cellular biological effects was also analyzed. Results: After mechanical strain application, cell proliferation significantly increased on days 5 and 7 (p < 0.05), and COL2A1 and ACAN gene expression levels were upregulated (p < 0.05). Protein levels of Collagen II and Aggrecan significantly increased, while MMP-13 levels decreased (p < 0.05). Upstream signaling molecules such as Integrin β1, TGF-β1, and WNT3A were upregulated (p < 0.05), while TGFBR2 showed no significant changes (p > 0.05). Downstream molecules p-ERK, MAPK1, and MAPK14 were significantly upregulated (p < 0.05), whereas p-p38 and β-catenin showed no significant differences (p > 0.05). ACAN and p-ERK expression levels exhibited a dose-response relationship with increasing strain amplitude (p < 0.05). Conclusion: Mechanical load promotes the proliferation and matrix synthesis of pediatric hip cartilage cells through specific regulation of upstream and downstream signaling pathway molecules, showing strain intensity-dependent molecular responses. This study lays the foundation for precision regulation strategies in cartilage development and repair.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"22 5","pages":"1240-1240"},"PeriodicalIF":0.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ojs.sin-chn.com/index.php/mcb/article/download/1240/1046","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147892674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of a flexible glucose electrochemical sensor and its detection in elderly diabetic patients","authors":"Ning Ma, Yang Song, Lisha Zhang","doi":"10.62617/mcb1453","DOIUrl":"https://doi.org/10.62617/mcb1453","url":null,"abstract":"The study proposes a diabetes detection scheme based on a novel flexible glucose electrochemical sensor for the current situation of diabetes management in China, especially for the elderly diabetic population. The sensor is fabricated using optimized conductive materials and diluents with set printing parameters. It mainly realizes non-invasive monitoring of human blood glucose by detecting human sweat, thus effectively detecting elderly diabetic patients. Simulation experiments showed that the sensor had a detection limit of 7.33 μM (S/N = 3) and a high sensitivity of 23.5 μA mm−2 for simulated sweat, demonstrating good stability and durability. Moreover, in the actual in vitro detection experiments, the sensor detected elderly diabetic patients with an accuracy of more than 98.3%. In addition, the response time of the sensor was very short, only 10.5 s to complete a detection, which was suitable for elderly patients. The above illustrated that the flexible glucose electrochemical sensor prepared in the research had certain feasibility and accuracy in the blood glucose monitoring of elderly diabetic patients. The research results not only provide theoretical basis and technical support for the preparation of flexible glucose electrochemical sensors, but also provide new ideas and methods for blood glucose monitoring and treatment of elderly diabetic patients.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"22 5","pages":"1453-1453"},"PeriodicalIF":0.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ojs.sin-chn.com/index.php/mcb/article/download/1453/1110","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147880022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomechanical influence of organic matter enrichment and sedimentary environment in the Ordos Basin","authors":"Siyuan Feng, Yu Zhang, Bingyuan Wu","doi":"10.62617/mcb1251","DOIUrl":"https://doi.org/10.62617/mcb1251","url":null,"abstract":"To study the correlation between organic matter enrichment and sedimentary environment in the Ordos Basin, hydrocarbon source rocks in the region of the eastern edge of the central plain of the basin are selected for analysis. With reference to the related theories of sedimentary rocks and organic geochemistry, the means including pyrolyzed rocks, organic carbon determination, and detection of main trace elements are adopted. The relationship between the regional sedimentary environment and organic matter enrichment is examined after the features of the paleo-sedimentary environment in the study area are examined. At the same time, from the perspective of biomechanics, the concepts of fluid mechanics and material transport are introduced to analyze the influence of hydrodynamic conditions in sedimentary environments on the migration and enrichment of organic matter. The following results were obtained through the study of organic matter enrichment and sedimentary environment in the Ordos Basin: (1) In the analysis of microscopic features, the study area is mainly composed of matrix vitrinite (accounting for more than 68%) and sericite, and also found woody fibers, microbial debris, and pyrite. (2) In organic matter analysis, it was found that the hydrocarbon generation potential (S1 + S2) of the B-S group was divided into multiple levels. According to the relationship, coal can be classified as a good source rock, while the carbonaceous mudstone in the S group belongs to a poor source rock. Group B-T is mainly composed of type III kerogen (humic type). (3) In the analysis of geochemical indicators, the concentrations of Si, Al, and Fe are very high. Further analysis shows that Si and Ti are the main enriched elements, while Re and Mo are abnormally enriched elements. (4) In the analysis of sample physical properties, sample characterization indicates that the source rock is mainly microporous, with few micropores in sandstone, mainly consisting of fractures and medium to large pores. Research has found that there is a correlation between the pore conditions of the sample and organic matter, and the pore volume increases relatively with the increase of organic matter. (5) Organic matter, abdominal muscles, and sediment environment analysis, according to the examination of geochemical indicators, the research area’s environment changed significantly from humid to arid and semi-arid conditions at various points during the early, middle, and end of the study period. The salinity of the water body in different regions differed significantly, which was related to the regional climate. The study of the carbon content of organic matter and the characteristics of the sedimentary environment displayed that the redox reaction in the early stages of the region and the local climate had a major influence on the enrichment of organic matter. These two factors became the primary controlling factors affecting the region’s organic matter enrichment. The investiga","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"22 4","pages":"1251-1251"},"PeriodicalIF":0.0,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ojs.sin-chn.com/index.php/mcb/article/download/1251/1026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147916626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of dynamic monitoring of blood routine indexes on ECG characteristics of elderly patients with diabetes and the application of sensor technology","authors":"Zhang Lisha, Yan Yang, Ning Ma","doi":"10.62617/mcb1451","DOIUrl":"https://doi.org/10.62617/mcb1451","url":null,"abstract":"To provide a more efficient and real-time blood routine monitoring method for elderly patients with diabetes, and to explore the correlation between blood routine indexes and Electrocardiogram (ECG) characteristics. A real-time blood routine detection device based on electrochemical sensor was designed, and a portable instrument based on optical sensor was developed to monitor trace biomarkers in blood using a labeled electrochemical biogold nanoparticle sensor. The results show that the sensor can monitor bilirubin level in real time, and the correlation coefficient with blood routine results is 0.95, so that the clinical monitoring time is shortened from 2 h to 30 min. The detection limit of white blood cell count was 0.85 × 109/L, and the data collection rate was 50 times/s, which improved the detection accuracy by 15.8% compared with the traditional laboratory method. In addition, the study revealed the mechanism of potential influence of changes in blood routine indicators on ECG characteristics. The monitoring device in this study can reduce the cost, improve the efficiency, and provide a precise and convenient clinical application scheme for the health management of elderly diabetes patients.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"22 4","pages":"1451-1451"},"PeriodicalIF":0.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ojs.sin-chn.com/index.php/mcb/article/download/1451/973","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147887749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of indoor building air microplastics on human living environment health: A biomechanical perspective","authors":"Jing Liang, Liang Zhou, Q.G. Wang, Yong Tian, Ting Tian, Hongjun Xiang","doi":"10.62617/mcb1371","DOIUrl":"https://doi.org/10.62617/mcb1371","url":null,"abstract":"Introduction: Microplastics are plastic particles less than 5 mm in diameter, mainly from synthetic textiles, home decoration materials, cleaning supplies and plastic products wear. These microplastics can enter the body through respiratory inhalation, skin contact or dietary ingestion, posing a potential threat to human health. Studies have found that inhaling microplastics can trigger respiratory inflammation, allergic reactions, and even chronic respiratory diseases (such as bronchitis and asthma). Microplastic particles can accumulate in the lungs, and long-term exposure can exacerbate respiratory diseases. In addition, microplastics may also enter other organs through the blood circulation, affecting the immune system and nervous system function. In the indoor environment, the release of microplastics is closely related to daily activities, with higher concentrations of microplastics in high-frequency activity areas (such as living rooms) and greater exposure risks. Therefore, the health effects of indoor microplastic pollution on long-term residents should not be ignored, and further research on its long-term health effects and measures to reduce exposure risks are needed. Objectives: To precisely determine the concentration levels of microplastics in indoor air and comprehensively assess their potential risks to human health, with a focus on how these microplastics interact with the biomechanical aspects of the human body. Methods: This study explores the impact of indoor building air microplastics on human environmental health and analyzes the human exposure risk of microplastic distribution in different regions. Results: The results showed that the microplastic content in the living room area was 241 ± 21 n/m3, with the highest content, while the kitchen had the lowest. In the assessment of human exposure risk, subjects had the highest daily and annual exposure levels in the living room, with some experiencing symptoms such as allergies and coughing, indicating moderate exposure risk. The daily average exposure of subjects P1 and P5 could reach 1364MPS/day and 1142MPS/day, with an average annual exposure of 1,124,000 and 1,214,000 particles, respectively. Microplastics in indoor air are mainly small particles of 20–100 microns, mainly in the form of fragments, and synthetic rubber and packaging plastics are the most common types. Health risk assessment shows that individuals exposed to high concentrations of microplastics for a long time are prone to allergies, mild cough and other problems, and exposure time is negatively correlated with health scores. Daily and annual exposure levels varied significantly by region, with the living room highest and the kitchen lowest. Conclusion: The study provides quantitative data on indoor exposure levels of microplastics and provides a scientific basis for assessing their health risks. The potential harm mechanism of microplastics to respiratory tract was revealed from the perspective of biomechanic","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"22 4","pages":"1371-1371"},"PeriodicalIF":0.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ojs.sin-chn.com/index.php/mcb/article/download/1371/969","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147919030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fe-doped polyoxometalates nanoclusters for near-infrared mild-temperature photothermal bacterial disinfection: A biomechanical perspective","authors":"Jing Zhou, Mingzhu Lv, Xinyi Wang, Yuan Yong, Guobo Du, Xiaoming Wang","doi":"10.62617/mcb1475","DOIUrl":"https://doi.org/10.62617/mcb1475","url":null,"abstract":"Currently, multidrug-resistant (MDR) pathogens are becoming a human and economic burden worldwide and have posed a grave threat to public health. In this case, mild-temperature photothermal therapy (PTT) has become a promising alternative to conventional antibiotics because it has the characteristics of minimally invasive property, targeted destructiveness, and low toxicity. To realize effective photothermal therapy, it is imperative to fabricate multifunctional photothermal formulations with better performance. In this study, novel Fe-doped polyoxometalate (Fe-POM) nanoclusters were successfully prepared by the one-pot method. Due to the strong absorption efficiency in the near-infrared (NIR) region, high photothermal conversion efficiency, and photothermal cycling stability, Fe-POM nanoclusters are sufficient to be used as an efficient photothermal agent for highly effective mild-temperature photothermal therapy. From a biomechanical perspective, the Fe-POM nanoclusters not only generate heat under NIR irradiation but also produce hydroxyl radicals (·OH) through the redox cycling of Fe2+/Fe3+ and Mo5+/Mo6+ pairs. The reactive oxygen species (ROS) generated disrupt bacterial cell membranes and alter their biomechanical properties, such as membrane stiffness and permeability, rendering the bacteria more susceptible to mild-temperature PTT. The synergistic effect of ROS-induced biomechanical stress and photothermal heating significantly enhances bacterial disinfection. Antibacterial experiments demonstrated that Fe-POM nanoclusters, under PTT conditions, effectively induce bacterial death while maintaining good biocompatibility. This study highlights the potential of Fe-POM nanoclusters as a multifunctional photothermal agent for combating bacterial infections. This work not only advances the field of photothermal therapy but also provides a novel strategy for treating bacteria-associated diseases through biomechanical modulation.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"22 4","pages":"1475-1475"},"PeriodicalIF":0.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ojs.sin-chn.com/index.php/mcb/article/download/1475/949","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147922487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockdown of DNAJC12 slows tumor progression and affects tumor radiosensitivity in esophageal squamous cell carcinoma","authors":"Xiao Ju, Jianbo Zhang, Linke Yang, Pei Li, Ping Wang","doi":"10.62617/mcb1534","DOIUrl":"https://doi.org/10.62617/mcb1534","url":null,"abstract":"Purpose: To look into the influence of DNAJC12 knockdown on the progression and radio-sensitivity of esophageal squamous cell carcinoma (ESCC), with a focus on cellular mechanics and tumor microenvironment interactions. Methods: The TCGA database combined with immunohistochemical staining was used to validate the DNAJC12 expression in ESCC patients from the perspective of the clinic. DNAJC12 knockdown was performed in TE-1 and KYSE-150 cell lines to assess changes in proliferation, migration, invasion, apoptosis, and cellular mechanical properties (e.g., stiffness, adhesion, and contractility). The downstream molecule regulated by DNAJC12 was explored using Western blotting and biomechanical assays. The effect of DNAJC12 knockdown on tumor radiosensitivity was evaluated in vivo, with a focus on tumor stiffness and extracellular matrix (ECM) remodeling under irradiated conditions. Results: Upon analyzing the TCGA database and examining tumor tissue samples from patients, it was discovered that DNAJC12 exhibited high expression levels in tissues of ESCC. Vitro experiments showed that DNAJC12 knockdown significantly decreased cellular proliferation and migration (P < 0.05). Biomechanical assays revealed that DNAJC12 knockdown decreased cellular stiffness and contractility, suggesting a role in regulating cytoskeletal dynamics. Molecular analysis showed downregulation of P-ERK, MMP-2, N-Cadherin, P-P38, Snail, Vimentin, β-Catenin, Fibronectin, and Twist alongside upregulation of E-Cadherin (P < 0.05). Overexpression of SNAI1 could restore the proliferative and migratory capabilities of cells with downregulated DNAJC12. In vivo experiments, knockdown of DNAJC12 resulted in faster tumor growth under irradiated conditions (P < 0.05). Conclusion: DNAJC12 knockdown slows ESCC progression by modulating cellular biomechanical properties and molecular pathways. However, it enhances tumor growth post-radiotherapy, potentially due to altered mechanosensitive signaling and ECM remodeling. These findings highlight the interplay between molecular biology and biomechanics in ESCC progression and treatment response.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"22 4","pages":"1534-1534"},"PeriodicalIF":0.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ojs.sin-chn.com/index.php/mcb/article/download/1534/933","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147897718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanical characterization of hyaluronic acid-modified cationic liposomes for targeted deliver of ONECUT2 shRNA in hepatocellular carcinoma","authors":"Shifeng Liu, Wenli Zhao, Xinran Song, Qing Li, Ligang Zhang, Ning Deng","doi":"10.62617/mcb1543","DOIUrl":"https://doi.org/10.62617/mcb1543","url":null,"abstract":"Hepatocellular carcinoma (HCC) is a globally significant malignancy with high morbidity and mortality. Anti-tumor targeted drug therapy is a promising therapeutic strategy, but the strategy faces challenges related to delivery efficiency and mechanical interactions within the tumor microenvironment. In our previous study, we found that the transcription factors ONECUT2 (OC2) and CD44 receptor have important roles in HCC progression. We designed high molecular weight hyaluronic acid-modified cationic liposomes (HMW-CL) to take advantage of the binding affinity between hyaluronic acid and CD44 to deliver plasmid DNA (pshOC2) encoding a short hairpin RNA targeting OC2 to HCC cells. The results showed that the prepared HMW-CL had a uniform particle size of 179.5 nm, a moderate zeta potential of 15.8 mV, a high encapsulation efficiency of 86%, which not only protected pshOC2 from degradation but also ensured favorable mechanical stability under physiological shear stresses. Biomechanical characterization revealed that the liposomes maintained structural integrity under simulated blood flow conditions, with minimal deformation and optimal adhesion to CD44-expressing HCC cells. In vitro experiments, HMW-CL/pshOC2 liposomes were characterized by high transfection efficacy, lysosomal escape, and low cytotoxicity. They could efficiently deliver pshOC2 to cells, affecting HCC cell proliferation, migration, invasion, and triggering apoptosis. Biomechanical assays further confirmed that the liposomes altered the mechanical properties of HCC cells, reducing their stiffness and migratory capacity, which are critical factors in tumor progression. In vivo experiments, intravenous injection of HMW-CL/pshOC2 liposomes effectively reduced OC2 expression in HCC tumors and inhibited tumor growth at low toxicity with an inhibition rate of 81.77%. Our study demonstrated that OC2 may be a candidate gene suitable for HCC targeted-therapy, and our HMW-CL/pshOC2 liposomes were prepared based on the hyaluronic acid/CD44 binding strategy, with good stability, high transfection efficacy, and low cytotoxicity. Moreover, their favorable biophysical and biomechanical properties make them a promising delivery system for HCC therapy, with potential applications in modulating the mechanical microenvironment of tumors.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"22 4","pages":"1543-1543"},"PeriodicalIF":0.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ojs.sin-chn.com/index.php/mcb/article/download/1543/921","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147894578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Based on SLC7A11/GPX4 signaling pathway, the mechanism of inhibiting cell iron death in the treatment of asthma was investigated","authors":"Yueyang Wang, Xiangming Fang, Weidong Ye","doi":"10.62617/mcb1391","DOIUrl":"https://doi.org/10.62617/mcb1391","url":null,"abstract":"Objective: To investigate the effects of Pingchuanning prescription (PCN) and Ferrostatin 1 inhibitors on airway inflammation in asthmatic rats from the perspective of cell iron death. Methods: Seventy SD rats were randomly divided into 7 groups: normal group, model group, Pinbuening group, Ferrostatin 1 inhibitor group, Pinbuening + Ferrostatin 1 inhibitor group, dexamethasone group, and Guilong Kechuanning group. 10% chicken egg albumin (OVA) was sensitized by peritoneal and limb subcutaneous injection. The asthmatic rat model was stimulated by 2% OVA atomization combined with cold (2–4 ℃) air stimulation. Pingchuanning (6.43 g/kg), Ferrostatin-1 (10 mg/kg), Pingchuanning (6.43 g/kg) + Ferrostatin-1 (2.5 μmol/kg), dexamethasone (0.5 g/kg), Guilong Kecchuanning (10g/kg) by gavage and atomization, Continuous intervention for 3 weeks. After the last stimulation, the lung tissues of rats were stained with hematoxylin-eosin (H&E) to observe airway inflammation and cell proliferation. The contents of IL-10, IL-22, IL-33 and ALOX15 in serum and LF of asthma were detected by enzyme-linked immunosorbent assay (ELISA). Real-time fluorescence quantitative polymerase chain reaction (RT-PCR) was used to detect the mRAN expression levels of SLC7A11 and GPX4, and Western blot was used to detect the protein expression levels of SLC7A11 and GPX4. Results: Compared with blank group, the diet, body weight, emotional irritability, respiratory shortness, airway inflammatory cell infiltration, goblet cell hyperplasia, serum and serum LF IL-10, IL-22, IL-33, ALOX15 inflammatory factors increased significantly in model group. The mRNA and protein expression levels of SLC7A11 and GPX4 were decreased. Compared with the model group, the diet of the rats in the Pinbuening, Ferrostatin 1 inhibitor and Pinbuening +Ferrostatin 1 inhibitor groups was gradually improved, wheezing was relieved, and airway inflammatory cell infiltration was significantly reduced. IL-10, IL-22, IL-33 and ALOX15 inflammatory factors in serum and LF of asthma were decreased (P < 0.001), while the mRNA and protein expressions of SLC7A11 and GPX4 were promoted (P < 0.005). Conclusions: Pinbuterin and its Ferrostatin 1 inhibitors can significantly improve airway inflammation induced by OVA combined with cold stimulation in asthmatic rats, and are related to SLC7A11/GPX4 signaling pathway and cell iron death. The efficacy of Pinbuterin combined with Ferrostatin 1 inhibitors is more obvious. It is suggested that the effect of combined treatment is better than that of single compound or western medicine.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"22 4","pages":"1391-1391"},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ojs.sin-chn.com/index.php/mcb/article/download/1391/897","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}