Biosensors-Basel最新文献

{"title":"Impedimetric Biosensors for the Quantification of Serum Biomarkers for Early Detection of Lung Cancer.","authors":"Mahdi Arabnejad, Ibtisam E Tothill, Iva Chianella","doi":"10.3390/bios14120624","DOIUrl":"10.3390/bios14120624","url":null,"abstract":"<p><p>Lung cancer is the most common type of cancer diagnosed worldwide and is also among the most fatal. Early detection, before symptoms become evident, is fundamental for patients' survival. Therefore, several lung cancer biomarkers have been proposed to enable a prompt diagnosis, including neuron-specific enolase (NSE) and carcinoembryonic antigen (CEA). NSE and CEA are two serum proteins whose elevated levels have been associated with lung cancer. Hence, in this study, impedimetric biosensors (immunosensors) able to quantify NSE and CEA were developed as proof-of-concept devices for lung cancer diagnosis. The sensing platform exploited for the immunosensors comprises a novel combination of a magnetic platform, screen-printed gold electrode (SPGE), and magnetic nanobeads (MB). The MB were functionalized with antibodies to capture the analyte from the sample and to move it over the sensing area. The immunosensors were then developed by immobilizing another set of antibodies for either CEA or NSE on the SPGE through formation of self-assembled monolayer (SAM). The second set of antibodies enabled a sandwich assay to be formed on the surface of the sensor, while MB manipulation was applied during the sensor performance to depict a microfluidic system and increase antigen-antibody complex formation prior to CEA or NSE detection and quantification. The optimized immunosensors were successfully tested to measure various concentrations of CEA and NSE (0-100 ng/mL) in both phosphate buffer and 100% human serum samples. Clinically relevant detection limits of 0.26 ng/mL and 0.18 ng/mL in buffer and 0.76 ng/mL and 0.52 ng/mL in 100% serum for CEA and NSE, respectively, were achieved via electrochemical impedance spectroscopy with the use of potassium ferri/ferrocyanide as a redox probe. Hence, the two immunosensors demonstrated great potential as tools to be implemented for the early detection of lung cancer.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"14 12","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wearable Fabric System for Sarcopenia Detection.","authors":"Zhenhe Huang, Qiuqian Ou, Dan Li, Yuanyi Feng, Liangling Cai, Yue Hu, Hongwei Chu","doi":"10.3390/bios14120622","DOIUrl":"10.3390/bios14120622","url":null,"abstract":"<p><p>Sarcopenia has been a serious concern in the context of an increasingly aging global population. Existing detection methods for sarcopenia are severely constrained by cumbersome devices, the necessity for specialized personnel, and controlled experimental environments. In this study, we developed an innovative wearable fabric system based on conductive fabric and flexible sensor array. This fabric system demonstrates remarkable pressure-sensing capabilities, with a high sensitivity of 18.8 kPa^-1 and extraordinary stability. It also exhibits excellent flexibility for wearable applications. By interacting with different parts of the human body, it facilitates the monitoring of various physiological activities, such as pulse dynamics, finger movements, speaking, and ambulation. Moreover, this fabric system can be seamlessly integrated into sole to track critical indicators of sarcopenia patients, such as walking speed and gait. Clinical evaluations have shown that this fabric system can effectively detect variations in indicators relevant to sarcopenia patients, proving that it offers a straightforward and promising approach for the diagnosis and assessment of sarcopenia.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"14 12","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Continuous Estimation of Blood Pressure by Utilizing Seismocardiogram Signal Features in Relation to Electrocardiogram.","authors":"Aleksandra Zienkiewicz, Vesa Korhonen, Vesa Kiviniemi, Teemu Myllylä","doi":"10.3390/bios14120621","DOIUrl":"10.3390/bios14120621","url":null,"abstract":"<p><p>There is an ongoing search for a reliable and continuous method of noninvasive blood pressure (BP) tracking. In this study, we investigate the feasibility of utilizing seismocardiogram (SCG) signals, i.e., chest motion caused by cardiac activity, for this purpose. This research is novel in examining the temporal relationship between the SCG-measured isovolumic moment and the electrocardiogram (PEP_IM). Additionally, we compare these results with the traditionally measured pre-ejection period with the aortic opening marked as an endpoint (PEP_AO). The accuracy of the BP estimation was evaluated beat to beat against invasively measured arterial BP. Data were collected on separate days as eighteen sets from nine subjects undergoing a medical procedure with anesthesia. Results for PEP_IM showed a correlation of 0.67 ± 0.18 (<i>p</i> < 0.001), 0.66 ± 0.17 (<i>p</i> < 0.001), and 0.67 ± 0.17 (<i>p</i> < 0.001) when compared to systolic BP, diastolic BP, and mean arterial pressure (MAP), respectively. Corresponding results for PEP_AO were equal to 0.61 ± 0.22 (<i>p</i> < 0.001), 0.61 ± 0.21 (<i>p</i> < 0.001), and 0.62 ± 0.22 (<i>p</i> < 0.001). Values of PEP_IM were used to estimate MAP using two first-degree models, the linear regression model (achieved RMSE of 11.7 ± 4.0 mmHg) and extended model with HR (RMSE of 10.8 ± 4.2 mmHg), and two corresponding second-degree models (RMSE of 10.8 ± 3.7 mmHg and RMSE of 8.5 ± 3.4 mmHg for second-degree polynomial and second-degree extended, respectively). In the intrasubject testing of the second-degree model extended with HR based on PEP_IM values, the mean error of MAP estimation in three follow-up measurements was in the range of 7.5 to 10.5 mmHg, without recalibration. This study demonstrates the method's potential for further research, particularly given that both proximal and distal pulses are measured in close proximity to the heart and cardiac output. This positioning may enhance the method's capacity to more accurately reflect central blood pressure compared to peripheral measurements.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"14 12","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paper-Based Aptasensor Assay for Detection of Food Adulterant Sildenafil.","authors":"Murat Kavruk, Veli Cengiz Ozalp","doi":"10.3390/bios14120620","DOIUrl":"10.3390/bios14120620","url":null,"abstract":"<p><p>Sildenafil is used to treat erectile dysfunction and pulmonary arterial hypertension but is often illicitly added to energy drinks and chocolates. This study introduces a lateral flow strip test using aptamers specific to sildenafil for detecting its illegal presence in food. The process involved using graphene oxide SELEX to identify high-affinity aptamers, which were then converted into molecular gate structures on mesoporous silica nanoparticles, creating a unique signaling system. This system was integrated into lateral flow chromatography strips and tested on buffers and chocolate samples containing sildenafil. The method simplifies the lateral flow assay (LFA) for small molecules and provides a tool for signal amplification. The detection limit for these strips was found to be 68.2 nM (31.8 µg/kg) in spiked food samples.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"14 12","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Entropy-Driven Molecular Beacon Assisted Special RCA Assay with Enhanced Sensitivity for Room Temperature DNA Biosensing.","authors":"Shurui Tao, Yi Long, Guozhen Liu","doi":"10.3390/bios14120618","DOIUrl":"10.3390/bios14120618","url":null,"abstract":"<p><p>The Phi29 DNA polymerase is renowned for its processivity in synthesizing single-stranded DNA amplicons by rolling around a circularized DNA template. However, DNA synthesis rolling circle amplification (RCA) is significantly hindered by the secondary structure in the circular template. To overcome this limitation, an engineered circular template without secondary structure could be utilized to improve the sensitivity of RCA-based assays without increasing its complexity. We herein proposed an entropy-driven special RCA technology for the detection of HPV16 E7 gene at room temperature. The strategy is composed of a molecular beacon containing a loop region for nucleic acid target recognition and a stem region to initiate RCA. With the target analyte, the stem region of the molecular beacon will be exposed and then hybridized with a special circular template to initiate the DNA amplification. We tested different designs of the molecular beacon sequence and optimized the assay's working conditions. The assay achieved a sensitivity of 1 pM in 40 min at room temperature. The sensitivity of this assay, at 1 pm, is about a hundred-fold greater than that of conventional linear RCA performed in solution. Our proposed sensor can be easily reprogrammed for detecting various nucleic acid markers by altering the molecular beacon's loop. Its simplicity, rapid assay time, and low cost make it superior to RCA sensors that utilize similar strategies.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"14 12","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"P300 Latency with Memory Performance: A Promising Biomarker for Preclinical Stages of Alzheimer's Disease.","authors":"Manal Mohamed, Nourelhuda Mohamed, Jae Gwan Kim","doi":"10.3390/bios14120616","DOIUrl":"10.3390/bios14120616","url":null,"abstract":"<p><p>Detecting and tracking the preclinical stages of Alzheimer's disease (AD) is now of particular interest due to the aging of the world's population. AD is the most common cause of dementia, affecting the daily lives of those afflicted. Approaches in development can accelerate the evaluation of the preclinical stages of AD and facilitate early treatment and the prevention of symptom progression. Shifts in P300 amplitude and latency, together with neuropsychological assessments, could serve as biomarkers in the early screening of declines in cognitive abilities. In this study, we investigated the ability of the P300 indices evoked during a visual oddball task to differentiate pre-clinically diagnosed participants from normal healthy adults (HCs). Two preclinical stages, named asymptomatic AD (AAD) and prodromal AD (PAD), were included in this study, and a total of 79 subjects participated, including 35 HCs, 22 AAD patients, and 22 PAD patients. A mixed-design ANOVA test was performed to compare the P300 indices among groups during the processing of the target and non-target stimuli. Additionally, the correlation between these neurophysiological variables and the neuropsychological tests was evaluated. Our results revealed that neither the peak amplitude nor latency of P300 can distinguish AAD from HCs. Conversely, the peak latency of P300 can be used as a biomarker to differentiate PAD from AAD and HCs. The correlation results revealed a significant relationship between the peak latency of P300 and memory domain tasks, showing that less time-demanding neuropsychological assessments can be used. In summary, our findings showed that a combination of P300 latency and memory-requiring tasks can be used as an efficient biomarker to differentiate individuals with AAD from HCs.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"14 12","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Active Surface-Enhanced Raman Scattering Platform Based on a 2D Material-Flexible Nanotip Array.","authors":"Yong Bin Kim, Satyabrat Behera, Dukhyung Lee, Seon Namgung, Kyoung-Duck Park, Dai-Sik Kim, Bamadev Das","doi":"10.3390/bios14120619","DOIUrl":"10.3390/bios14120619","url":null,"abstract":"<p><p>Two-dimensional materials with a nanostructure have been introduced as promising candidates for SERS platforms for sensing application. However, the dynamic control and tuning of SERS remains a long-standing problem. Here, we demonstrated active tuning of the enhancement factor of the first- and second-order Raman mode of monolayer (1L) MoS₂ transferred onto a flexible metallic nanotip array. Using mechanical strain, the enhancement factor of 1L MoS₂/nanotip is modulated from 1.23 to 8.72 for 2LA mode. For the same mode, the SERS intensity is enhanced by ~31 times when silver nanoparticles of ~13 nm diameter are deposited on 1L MoS₂/nanotip, which is tuned up to ~34 times by compressive strain. The change in SERS enhancement factor is due to the decrease (increase) in gap width as the sample is bent inwardly (outwardly). This is corroborated by FEM structural and electromagnetic simulation. We also observed significant control over mode peak and linewidth, which may have applications in biosensing, chemical detection, and optoelectronics.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"14 12","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674311/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Highly Stable Electrochemical Sensor Based on a Metal-Organic Framework/Reduced Graphene Oxide Composite for Monitoring the Ammonium in Sweat.","authors":"Yunzhi Hua, Junhao Mai, Rourou Su, Chengwei Ma, Jiayi Liu, Cong Zhao, Qian Zhang, Changrui Liao, Yiping Wang","doi":"10.3390/bios14120617","DOIUrl":"10.3390/bios14120617","url":null,"abstract":"<p><p>The demand for non-invasive, real-time health monitoring has driven advancements in wearable sensors for tracking biomarkers in sweat. Ammonium ions (NH₄⁺) in sweat serve as indicators of metabolic function, muscle fatigue, and kidney health. Although current ion-selective all-solid-state printed sensors based on nanocomposites typically exhibit good sensitivity (~50 mV/log [NH₄⁺]), low detection limits (LOD ranging from 10^-6 to 10^-7 M), and wide linearity ranges (from 10^-5 to 10^-1 M), few have reported the stability test results necessary for their integration into commercial products for future practical applications. This study presents a highly stable, wearable electrochemical sensor based on a composite of metal-organic frameworks (MOFs) and reduced graphene oxide (rGO) for monitoring NH₄⁺ in sweat. The synergistic properties of Ni-based MOFs and rGO enhance the sensor's electrochemical performance by improving charge transfer rates and expanding the electroactive surface area. The MOF/rGO sensor demonstrates high sensitivity, with a Nernstian response of 59.2 ± 1.5 mV/log [NH₄⁺], an LOD of 10^-6.37 M, and a linearity range of 10^-6 to 10^-1 M. Additionally, the hydrophobic nature of the MOF/rGO composite prevents water layer formation at the sensing interface, thereby enhancing long-term stability, while its high double-layer capacitance minimizes potential drift (7.2 µV/s (i = ±1 nA)) in short-term measurements. Extensive testing verified the sensor's exceptional stability, maintaining consistent performance and stable responses across varying NH₄⁺ concentrations over 7 days under ambient conditions. On-body tests further confirmed the sensor's suitability for the continuous monitoring of NH₄⁺ levels during physical activities. Further investigations are required to fully elucidate the impact of interference from other sweat components (such as K⁺, Na⁺, Ca²⁺, etc.) and the influence of environmental factors (including the subject's physical activity, posture, etc.). With a clearer understanding of these factors, the sensor has the potential to emerge as a promising tool for wearable health monitoring applications.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"14 12","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674821/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Massive Screening of Food Extracts for Quality Assessment and Standardization of Allergenic Activity.","authors":"Luis Antonio Tortajada-Genaro","doi":"10.3390/bios14120615","DOIUrl":"10.3390/bios14120615","url":null,"abstract":"<p><p>(1) Background: In drug discovery and pharmaceutical quality control, a challenge is to assess protein extracts used for allergy therapy and in vivo diagnosis, such as prick tests. Indeed, there are significant differences between the features of marketed products due to variations in raw materials, purification processes, and formulation techniques. (2) Methods: A protein array technology has been developed to provide comprehensive information on protein-biomarker interactions on a large scale to support the pharmaceutical industry and clinical research. The biosensing method is based on immobilizing low volumes of protein extracts (40 nL) on thermoplastic chips in array format. The biological activity was estimated by incubating with serum from representative food allergy patients. (3) Results: The reproducible optical signals were registered (deviation lower than 10%) using low-cost technologies such as a smartphone and a reader of digital versatile discs. The method was applied to pharmaceutical products to diagnose ten common food allergies, including barley, kiwi, milk, prawn, egg, peanut, wheat, peach, walnut, and squid. Quality indicators were established from spot intensities, enabling an effective comparison of manufacturers. (4) Conclusions: A biosensing-based strategy for screening pharmaceutical products emerges as a reliable and advantageous alternative to traditional approaches such as electrophoresis, fluorescence chips, and ELISA assays. This high-throughput method can contribute to understanding complex biological processes and evaluate the performance of pharmaceutical products.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"14 12","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensing Platform Based on Gold Nanoclusters and Nanoporous Anodic Alumina for Preeclampsia Detection.","authors":"Josep Maria Cantons, Akash Bachhuka, Lluis F Marsal","doi":"10.3390/bios14120610","DOIUrl":"10.3390/bios14120610","url":null,"abstract":"<p><p>Preeclampsia is a pregnancy-specific hypertensive syndrome recognized as the leading cause of maternal and fetal morbidity worldwide. Early diagnosis is crucial for mitigating its adverse effects, and recent investigations have identified endoglin as a potential biomarker for this purpose. Here, we present the development of a hybrid biosensor platform for the ultrasensitive detection of endoglin, aimed at enabling the early diagnosis of preeclampsia. This platform integrates the high surface area properties of nanoporous anodic alumina (NAA) with the unique optical characteristics of gold nanoclusters (AuNCs) to achieve enhanced detection capabilities. The NAA surface functionalized to promote attachment of AuNCs, which then was functionalized with specific antibodies to confer selectivity towards endoglin. Photoluminescence (PL) analysis of the biosensor demonstrated a linear detection range of 10-50 ng/mL, with a detection limit of 5.4 ng/mL and a sensitivity of 0.004 a.u./(ng/mL). This proof-of-concept study suggests that the NAA-AuNCs-based biosensing platform holds significant potential for the development of ultrasensitive, portable, and cost-effective diagnostic tools for preeclampsia, offering a promising avenue for advancing prenatal care.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"14 12","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}