Biosensors-Basel最新文献_第9页

Enhanced Transdermal Delivery of Lidocaine Hydrochloride via Dissolvable Microneedles (LH-DMNs) for Rapid Local Anesthesia. 可溶微针（LH-DMNs）增强盐酸利多卡因快速局部麻醉的经皮给药。

IF 5.6 3区工程技术

Biosensors-Basel Pub Date : 2025-08-21 DOI: 10.3390/bios15080552

Shengtai Bian, Jie Chen, Ran Chen, Shilun Feng, Zizhen Ming

{"title":"Enhanced Transdermal Delivery of Lidocaine Hydrochloride via Dissolvable Microneedles (LH-DMNs) for Rapid Local Anesthesia.","authors":"Shengtai Bian, Jie Chen, Ran Chen, Shilun Feng, Zizhen Ming","doi":"10.3390/bios15080552","DOIUrl":"https://doi.org/10.3390/bios15080552","url":null,"abstract":"Microneedles represent an emerging transdermal drug delivery platform offering painless, minimally invasive penetration of the stratum corneum. This study addresses limitations of conventional lidocaine hydrochloride formulations, such as slow onset and poor patient compliance, by developing lidocaine hydrochloride-loaded dissolvable microneedles (LH-DMNs) for rapid local anesthesia. LH-DMNs were fabricated via centrifugal casting using polyvinyl alcohol (PVA) as the matrix material in polydimethylsiloxane (PDMS) negative molds, which imparts high mechanical strength to the microneedles. Biocompatibility assessments showed negligible skin irritation, resolving within 3 min. And drug-loading capacity reached 24.0 ± 2.84 mg per patch. Pharmacodynamic evaluation via mouse hot plate tests demonstrated significant analgesia, increasing paw withdrawal latency to 36.11 ± 1.62 s at 5 min post-application (p < 0.01). The results demonstrated that the LH-DMNs significantly elevated the pain threshold in mice within 5 min, surpassing the efficacy of conventional anesthetic gels and providing a rapid and effective solution for pain relief. These findings validate the system's rapid drug release and efficacy, positioning dissolvable microneedles as a clinically viable alternative for enhanced transdermal anesthesia.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 8","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12384602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144975077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preliminary Study Using Wearable Near-Infrared Spectroscopy for Continuous Monitoring of Hemodynamics Through the Carotid Artery. 可穿戴近红外光谱连续监测颈动脉血流动力学的初步研究。

IF 5.6 3区工程技术

Biosensors-Basel Pub Date : 2025-08-20 DOI: 10.3390/bios15080549

Nisha Maheshwari, Alessandro Marone, Lokesh Sharma, Stephen Kim, Albert Favate, Andreas H Hielscher

{"title":"Preliminary Study Using Wearable Near-Infrared Spectroscopy for Continuous Monitoring of Hemodynamics Through the Carotid Artery.","authors":"Nisha Maheshwari, Alessandro Marone, Lokesh Sharma, Stephen Kim, Albert Favate, Andreas H Hielscher","doi":"10.3390/bios15080549","DOIUrl":"https://doi.org/10.3390/bios15080549","url":null,"abstract":"Non-invasive, continuous monitoring of carotid artery hemodynamics may provide valuable insights on cerebral blood perfusion (CBP). Near-infrared spectroscopy (NIRS) is a non-invasive modality that may be a good candidate for real-time carotid artery monitoring. We designed a wearable NIRS system to monitor the left and right radial and carotid arteries in 20 healthy subjects. The changes in total hemoglobin concentration (HbT) and tissue oxygen saturation (StO2) in all 80 arteries were continuously monitored in response to changes in oxygen supply. Wilcoxon non-parametric equivalence testing was used to compare changes in the radial (reference) and carotid arteries. The system-derived HbT and StO2 trends matched the expected physiological responses over time in the radial and carotid arteries. The mean peak-to-peak amplitude [uM] of HbT during sustained deep breathing was practically equivalent between the left radial (0.9 ± 0.8) and left carotid (1.6 ± 1.1) arteries (p = 0.01). The mean peak-to-peak amplitude [%] of StO2 was practically equivalent between the left radial (0.3 ± 0.2) and left carotid (0.3 ± 0.2) arteries (p < 0.001) and the right radial (0.4 ± 0.5) and right carotid (0.5 ± 0.4) arteries (p = 0.001). These findings indicate that NIRS may be a good option for monitoring the carotid arteries to track changes in CBP.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 8","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12384115/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144975014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protecting Firefighters from Carcinogenic Exposure: Emerging Tools for PAH Detection and Decontamination. 保护消防员免受致癌暴露：多环芳烃检测和净化的新兴工具。

IF 5.6 3区工程技术

Biosensors-Basel Pub Date : 2025-08-20 DOI: 10.3390/bios15080547

Morteza Ghafar-Zadeh, Azadeh Amrollahi Biyouki, Negar Heidari, Niloufar Delfan, Parviz Norouzi, Sebastian Magierowski, Ebrahim Ghafar-Zadeh

{"title":"Protecting Firefighters from Carcinogenic Exposure: Emerging Tools for PAH Detection and Decontamination.","authors":"Morteza Ghafar-Zadeh, Azadeh Amrollahi Biyouki, Negar Heidari, Niloufar Delfan, Parviz Norouzi, Sebastian Magierowski, Ebrahim Ghafar-Zadeh","doi":"10.3390/bios15080547","DOIUrl":"https://doi.org/10.3390/bios15080547","url":null,"abstract":"Polycyclic aromatic hydrocarbons (PAHs) are increasingly recognized as a major contributor to the occupational cancer risk among firefighters. In response, the National Fire Protection Association (NFPA) and other regulatory bodies have recommended rigorous decontamination protocols to minimize PAH exposure. Despite these efforts, a critical gap persists: the absence of real-time, field-deployable devices capable of detecting these invisible and toxic compounds during firefighting operations or within fire stations. Additionally, the lack of effective and optimized methods for the removal of these hazardous substances from the immediate environments of firefighters continues to pose a serious occupational health challenge. Although numerous studies have investigated PAH detection in environmental contexts, current technologies are still largely confined to laboratory settings and are unsuitable for field use. This review critically examines recent advances in PAH decontamination strategies for firefighting and explores alternative sensing solutions. We evaluate both conventional analytical methods, such as gas chromatography, high-performance liquid chromatography, and mass spectrometry, and emerging portable PAH detection technologies. By highlighting the limitations of existing systems and presenting novel sensing approaches, this paper aims to catalyze innovation in sensor development. Our ultimate goal is to inspire the creation of robust, field-deployable tools that enhance decontamination practices and significantly improve the health and safety of firefighters by reducing their long-term risks of cancer.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 8","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12384513/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144974981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrode-Free ECG Monitoring with Multimodal Wireless Mechano-Acoustic Sensors. 多模态无线机械声传感器的无电极心电监测。

IF 5.6 3区工程技术

Biosensors-Basel Pub Date : 2025-08-20 DOI: 10.3390/bios15080550

Zhi Li, Fei Fei, Guanglie Zhang

引用次数: 0

A Review of Machine Learning-Assisted Gas Sensor Arrays in Medical Diagnosis. 机器学习辅助气体传感器阵列在医学诊断中的研究进展。

IF 5.6 3区工程技术

Biosensors-Basel Pub Date : 2025-08-20 DOI: 10.3390/bios15080548

Yueting Yu, Xin Cao, Chenxi Li, Mingyue Zhou, Tianyu Liu, Jiang Liu, Lu Zhang

{"title":"A Review of Machine Learning-Assisted Gas Sensor Arrays in Medical Diagnosis.","authors":"Yueting Yu, Xin Cao, Chenxi Li, Mingyue Zhou, Tianyu Liu, Jiang Liu, Lu Zhang","doi":"10.3390/bios15080548","DOIUrl":"https://doi.org/10.3390/bios15080548","url":null,"abstract":"Volatile organic compounds (VOCs) present in human exhaled breath have emerged as promising biomarkers for non-invasive disease diagnosis. However, traditional VOC detection technology that relies on large instruments is not widely used due to high costs and cumbersome testing processes. Machine learning-assisted gas sensor arrays offer a compelling alternative by enabling the accurate identification of complex VOC mixtures through collaborative multi-sensor detection and advanced algorithmic analysis. This work systematically reviews the advanced applications of machine learning-assisted gas sensor arrays in medical diagnosis. The types and principles of sensors commonly employed for disease diagnosis are summarized, such as electrochemical, optical, and semiconductor sensors. Machine learning methods that can be used to improve the recognition ability of sensor arrays are systematically listed, including support vector machines (SVM), random forests (RF), artificial neural networks (ANN), and principal component analysis (PCA). In addition, the research progress of sensor arrays combined with specific algorithms in the diagnosis of respiratory, metabolism and nutrition, hepatobiliary, gastrointestinal, and nervous system diseases is also discussed. Finally, we highlight current challenges associated with machine learning-assisted gas sensors and propose feasible directions for future improvement.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 8","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12384702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144975133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosensor for Bacterial Detection Through Color Change in Culture Medium. 利用培养基颜色变化检测细菌的生物传感器。

IF 5.6 3区工程技术

Biosensors-Basel Pub Date : 2025-08-20 DOI: 10.3390/bios15080551

Aramis A Sánchez, Grettel Riofrío, Darwin Castillo, J P Padilla-Martínez, Vasudevan Lakshminarayanan

{"title":"Biosensor for Bacterial Detection Through Color Change in Culture Medium.","authors":"Aramis A Sánchez, Grettel Riofrío, Darwin Castillo, J P Padilla-Martínez, Vasudevan Lakshminarayanan","doi":"10.3390/bios15080551","DOIUrl":"https://doi.org/10.3390/bios15080551","url":null,"abstract":"Rapid and accurate bacterial detection is essential in medicine, the food industry, and environmental monitoring. This work presents the development of an optical sensor based on color changes in the culture medium that leverages the optical interaction of bacterial metabolic products. The proposed prototype operates on the principle of optical transmittance through mannitol salt agar (ASM), a selective medium for Staphylococcus aureus. As bacterial growth progresses, the medium undergoes changes in thickness and, primarily, color, which is optically measurable at specific wavelengths depending on the type of illumination provided by the simplified light-emitting diodes (LEDs). The sensor demonstrated the ability to detect bacterial growth in approximately 90-120 min, offering a significant reduction in detection time compared to traditional incubation methods. The system is characterized by its simplicity, sensitivity, low reagent consumption (up to 140 fewer reagents per test), and potential for real-time monitoring. These findings support the viability of the proposed sensor as an efficient alternative for early pathogen detection in both clinical and industrial applications. Finally, a proposal for simplifying the sensor in a system composed of a light-emitting diode and a light-dependent resistor is presented.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 8","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12384555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144975122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards an Implantable Aptamer Biosensor for Monitoring in Inflammatory Bowel Disease. 一种用于炎症性肠病监测的可植入适体生物传感器。

IF 5.6 3区工程技术

Biosensors-Basel Pub Date : 2025-08-19 DOI: 10.3390/bios15080546

Yanan Huang, Wenlu Duan, Fei Deng, Wenxian Tang, Sophie C Payne, Tianruo Guo, Ewa M Goldys, Nigel H Lovell, Mohit N Shivdasani

{"title":"Towards an Implantable Aptamer Biosensor for Monitoring in Inflammatory Bowel Disease.","authors":"Yanan Huang, Wenlu Duan, Fei Deng, Wenxian Tang, Sophie C Payne, Tianruo Guo, Ewa M Goldys, Nigel H Lovell, Mohit N Shivdasani","doi":"10.3390/bios15080546","DOIUrl":"https://doi.org/10.3390/bios15080546","url":null,"abstract":"Inflammatory bowel disease (IBD) is a relapsing-remitting condition resulting in chronic inflammation of the gastrointestinal tract. Present methods are either inadequate or not viable for continuous tracking of disease progression in individuals. In this study, we present the development towards an implantable biosensor for detecting interleukin-6 (IL-6), an important cytokine implicated in IBD. The optimised sensor design includes a gold surface functionalised with a known IL-6-specific aptamer, integrating a recognition sequence and an electrochemical redox probe. The IL-6 aptasensor demonstrated a sensitivity of up to 40% and selectivity up to 10% to the IL-6 target in vitro. Sensors were found to degrade over 7 days when exposed to recombinant IL-6, with the degradation rate rapidly increasing when exposed to intestinal mucosa. A feasibility in vivo experiment with a newly designed implantable gut sensor array confirmed rapid degradation over a 5-h implantation period. We achieved up to a 93% reduction in sensor degradation rates, with a polyvinyl alcohol-methyl acrylate hydrogel coating that aimed to reduce nonspecific interactions in complex analytes compared to uncoated sensors. Degradation was linked to desorption of the monolayer leading to breakage of gold thiol bonds. While there are key challenges to be resolved before a stable implantable IBD sensor is realised, this work highlights the potential of aptamer-based biosensors as effective tools for long-term diagnostic monitoring in IBD.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 8","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12384986/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144975069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design of an MIP-Based Electrochemical Sensor for the Determination of Paracetamol in Pharmaceutical Samples. 基于mip的药物样品中扑热息痛电化学传感器的设计。

IF 5.6 3区工程技术

Biosensors-Basel Pub Date : 2025-08-19 DOI: 10.3390/bios15080544

José Alberto Cabas Rodríguez, Fernando Javier Arévalo, Adrian Marcelo Granero

{"title":"Design of an MIP-Based Electrochemical Sensor for the Determination of Paracetamol in Pharmaceutical Samples.","authors":"José Alberto Cabas Rodríguez, Fernando Javier Arévalo, Adrian Marcelo Granero","doi":"10.3390/bios15080544","DOIUrl":"https://doi.org/10.3390/bios15080544","url":null,"abstract":"Paracetamol (PAR) is a common antipyretic and analgesic extensively used to treat cold and flu symptoms. It has been proven to be effective in headaches and relieving fever and pain. It is usually found as an over-the-counter drug, which has been associated with an increase in cases of poisoning due to overdose. Therefore, the development of new analytical tools for the detection of PAR at low concentrations in different samples is necessary. In this work, a Molecularly Imprinted Polymer (MIP)-based electrochemical sensor was designed for the selective and sensitive determination of PAR using a glassy carbon electrode (GCE) modified with a polymeric film obtained through the electropolymerization of o-aminophenol. A complete characterization based on electrochemical techniques, such as electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV), and scanning electron microscopy (SEM) was used to examine all steps involved in the construction of the MIP-based electrochemical sensor. In addition, all parameters affecting the MIP were optimized. As a result, the MIP-based electrochemical sensor showed a very low limit of detection (LOD) of 10 nM, with an analytical sensitivity of (3.4 ± 0.1) A M⁻¹. In addition, construction of the MIP-based electrochemical sensor showed highly reproducibility, expressed in terms of a variation coefficient lower than 4%. The MIP-based electrochemical sensor was successfully used in an assay for the determination of PAR in pharmaceutical products. The performance of the MIP-based electrochemical sensor was compared to High Performance Liquid Chromatography (HPLC) for the determination of PAR in pharmaceutical samples, showing excellent agreement between the two methodologies. A very important aspect of the developed sensor was its reusability for at least twenty times. The MIP-based electrochemical sensor is a reliable analytical tool for the determination of PAR.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 8","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12385103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144975094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Amperometric Enzyme-Nanozyme Biosensor for Glucose Detection. 用于葡萄糖检测的安培酶-纳米酶生物传感器。

IF 5.6 3区工程技术

Biosensors-Basel Pub Date : 2025-08-19 DOI: 10.3390/bios15080545

Asta Kausaite-Minkstimiene, Aiste Krikstaponyte, Nataliya Stasyuk, Galina Gayda, Almira Ramanaviciene

{"title":"An Amperometric Enzyme-Nanozyme Biosensor for Glucose Detection.","authors":"Asta Kausaite-Minkstimiene, Aiste Krikstaponyte, Nataliya Stasyuk, Galina Gayda, Almira Ramanaviciene","doi":"10.3390/bios15080545","DOIUrl":"https://doi.org/10.3390/bios15080545","url":null,"abstract":"Amperometric biosensors, due to their high sensitivity, fast response time, low cost, simple control, miniaturization capabilities, and other advantages, are receiving significant attention in the field of medical diagnostics, especially in monitoring blood glucose levels in diabetic patients. In this study, an amperometric glucose biosensor based on immobilized enzyme glucose oxidase (GOx) and bimetallic platinum cobalt (PtCo) nanoparticles was developed. The PtCo nanoparticles, deposited on a graphite rod electrode, exhibited peroxidase-like catalytic properties and were able to electrocatalyze the reduction of H2O2. After immobilization of the GOx, an amperometric signal generated by the biosensor was directly proportional to the glucose concentration in the range of 0.04-2.18 mM. The biosensor demonstrated a sensitivity of 19.38 μA mM-1 cm-2, with a detection limit of 0.021 mM and a quantification limit of 0.064 mM. In addition to this analytical performance, the biosensor exhibited excellent repeatability (relative standard deviation (RSD) was 4.90%); operational and storage stability, retaining 98.93% and 95.33% of its initial response after 26 cycles of glucose detection and over a 14-day period, respectively; and anti-interference ability against electroactive species, as well as exceptional selectivity for glucose and satisfactory reproducibility (RSD 8.90%). Additionally, the biosensor was able to detect glucose levels in blood serum with a high accuracy (RSD 5.89%), indicating potential suitability for glucose determination in real samples.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 8","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12384605/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144974927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence Imaging-Activated Microfluidic Particle Sorting Using Optical Tweezers. 荧光成像激活的光镊微流控颗粒分选。

IF 5.6 3区工程技术

Biosensors-Basel Pub Date : 2025-08-18 DOI: 10.3390/bios15080541

Yiming Wang, Xinyue Dai, Qingtong Jiang, Hangtian Fan, Tong Li, Xiao Xia, Yipeng Dou, Yuxin Mao

{"title":"Fluorescence Imaging-Activated Microfluidic Particle Sorting Using Optical Tweezers.","authors":"Yiming Wang, Xinyue Dai, Qingtong Jiang, Hangtian Fan, Tong Li, Xiao Xia, Yipeng Dou, Yuxin Mao","doi":"10.3390/bios15080541","DOIUrl":"https://doi.org/10.3390/bios15080541","url":null,"abstract":"The precise and efficient sorting of microscopic particles is critical in diverse fields, including biomedical diagnostics, drug development, and environmental monitoring. Fluorescence imaging-activated sorting refers to a strategy where fluorescence images are used to dynamically identify target particles and trigger selective manipulation for sorting purposes. In this study, we introduce a novel microfluidic particle sorting platform that combines optical tweezers with real-time fluorescence imaging for detection. High-speed image analysis enables accurate particle identification and classification, while the optical trap is selectively activated to redirect target particles. To validate the system's performance, we used 10 µm green and orange fluorescent polystyrene particles. The platform achieved a sorting purity of 94.4% for orange particles under continuous flow conditions. The proposed platform provides an image-based sorting solution, advancing the development of microfluidic systems for high-resolution particle sorting in complex biological and environmental applications.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 8","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12384192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144975175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0