Biosensors-Basel最新文献_第9页

Dual Monitoring of Blood Acetylcholinesterase Content and Catalytic Activity Utilizing Fluorometry-Integrated Surface Plasmon Resonance. 利用荧光测定法--集成表面等离子体共振对血液乙酰胆碱酯酶含量和催化活性进行双重监测

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-17 DOI: 10.3390/bios15020118

Yuanyuan Xie, Yifei Hou, Mengwei Hu, Hongzhuan Chen, Hao Wang, Lanxue Zhao, Jianrong Xu

{"title":"Dual Monitoring of Blood Acetylcholinesterase Content and Catalytic Activity Utilizing Fluorometry-Integrated Surface Plasmon Resonance.","authors":"Yuanyuan Xie, Yifei Hou, Mengwei Hu, Hongzhuan Chen, Hao Wang, Lanxue Zhao, Jianrong Xu","doi":"10.3390/bios15020118","DOIUrl":"10.3390/bios15020118","url":null,"abstract":"Acetylcholinesterase inhibitors (AChEIs), particularly donepezil, are commonly used to treat mild-to-moderate Alzheimer's disease (AD). However, drug accumulation during long-term use could change AChE activity and content, leading to peripheral side effects and prompting medication discontinuation. However, there are a lack of methods to simultaneously determine the content and catalytic activity of AChE. By using phosphatidylinositol-specific phospholipase C to strip AChE from erythrocyte surfaces, we developed a novel method combining surface plasmon resonance and fluorescence detection for the simultaneous detection of AChE content and activity, producing stable, reliable, and accurate results. The established determination range spans from 263.37 ng/mL to 3000 ng/mL (4.05 nM to 46.15 nM) for concentration, and from 39.02 mU/mL to 1000 mU/mL for activity. Compared to traditional methods, this approach simplifies operations, reduces detection time, expands the dynamic range, and lowers detection limits, potentially advancing AChE-related research and supporting clinical diagnostics and drug development.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11853072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transforming Sleep Monitoring: Review of Wearable and Remote Devices Advancing Home Polysomnography and Their Role in Predicting Neurological Disorders.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-17 DOI: 10.3390/bios15020117

Diana Vitazkova, Helena Kosnacova, Daniela Turonova, Erik Foltan, Martin Jagelka, Martin Berki, Michal Micjan, Ondrej Kokavec, Filip Gerhat, Erik Vavrinsky

{"title":"Transforming Sleep Monitoring: Review of Wearable and Remote Devices Advancing Home Polysomnography and Their Role in Predicting Neurological Disorders.","authors":"Diana Vitazkova, Helena Kosnacova, Daniela Turonova, Erik Foltan, Martin Jagelka, Martin Berki, Michal Micjan, Ondrej Kokavec, Filip Gerhat, Erik Vavrinsky","doi":"10.3390/bios15020117","DOIUrl":"10.3390/bios15020117","url":null,"abstract":"This paper explores the progressive era of sleep monitoring, focusing on wearable and remote devices contributing to advances in the concept of home polysomnography. We begin by exploring the basic physiology of sleep, establishing a theoretical basis for understanding sleep stages and associated changes in physiological variables. The review then moves on to an analysis of specific cutting-edge devices and technologies, with an emphasis on their practical applications, user comfort, and accuracy. Attention is also given to the ability of these devices to predict neurological disorders, particularly Alzheimer's and Parkinson's disease. The paper highlights the integration of hardware innovations, targeted sleep parameters, and partially advanced algorithms, illustrating how these elements converge to provide reliable sleep health information. By bridging the gap between clinical diagnosis and real-world applicability, this review aims to elucidate the role of modern sleep monitoring tools in improving personalised healthcare and proactive disease management.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11853583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Signal Differentiation of Moving Magnetic Nanoparticles for Enhanced Biodetection and Diagnostics. 移动磁性纳米粒子的信号分辨，用于增强生物检测和诊断。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-17 DOI: 10.3390/bios15020116

Kee Young Hwang, Dakota Brown, Supun B Attanayake, Dan Luu, Minh Dang Nguyen, T Randall Lee, Manh-Huong Phan

{"title":"Signal Differentiation of Moving Magnetic Nanoparticles for Enhanced Biodetection and Diagnostics.","authors":"Kee Young Hwang, Dakota Brown, Supun B Attanayake, Dan Luu, Minh Dang Nguyen, T Randall Lee, Manh-Huong Phan","doi":"10.3390/bios15020116","DOIUrl":"10.3390/bios15020116","url":null,"abstract":"Magnetic nanoparticles are extensively utilized as markers/signal labelling in various biomedical applications. Detecting and distinguishing magnetic signals from similarly sized moving magnetic nanoparticles in microfluidic systems is crucial yet challenging for biosensing. In this study, we have developed an original method to detect and differentiate magnetic signals from moving superparamagnetic (SPM) and ferrimagnetic (FM) nanoparticles of comparable sizes. Our approach utilizes a highly sensitive magnetic-coil-based sensor that harnesses the combined effects of giant magnetoimpedance (GMI) and an LC-resonance circuit, offering performance superior to that of conventional GMI sensors. Iron oxide nanoparticles, which have similar particle sizes but differing coercivities (zero for SPM and non-zero for FM) or similar zero coercivities but differing particle sizes, flow through the magnetic coil at controlled velocities. Their distinct effects are analyzed through changes in the complex impedance of the sensing system. Our findings provide a unique pathway for utilizing SPM and FM nanoparticles as innovative magnetic markers to identify specific biological entities, thereby expanding their potential applications.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11852982/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Ratiometric Fluorescence Nano pH Biosensor for Live-Cell Imaging Using Cerasome.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-16 DOI: 10.3390/bios15020114

Zhongqiao Zhang, Xiaoshan Luo, Xuanbo Wang, Meng Liu, Xiuli Yue, Zhaozhu Zheng

引用次数: 0

SERS-Active Micro/Nanomachines for Biosensing.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-16 DOI: 10.3390/bios15020115

Chenbing Li, Wenqing Zhang, Kai Zheng, Jianhe Guo

引用次数: 0

High-Accuracy Intermittent Strabismus Screening via Wearable Eye-Tracking and AI-Enhanced Ocular Feature Analysis. 通过可穿戴眼球跟踪和人工智能增强眼部特征分析进行高精度间歇性斜视筛查。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-14 DOI: 10.3390/bios15020110

Zihe Zhao, Hongbei Meng, Shangru Li, Shengbo Wang, Jiaqi Wang, Shuo Gao

{"title":"High-Accuracy Intermittent Strabismus Screening via Wearable Eye-Tracking and AI-Enhanced Ocular Feature Analysis.","authors":"Zihe Zhao, Hongbei Meng, Shangru Li, Shengbo Wang, Jiaqi Wang, Shuo Gao","doi":"10.3390/bios15020110","DOIUrl":"10.3390/bios15020110","url":null,"abstract":"An effective and highly accurate strabismus screening method is expected to identify potential patients and provide timely treatment to prevent further deterioration, such as amblyopia and even permanent vision loss. To satisfy this need, this work showcases a novel strabismus screening method based on a wearable eye-tracking device combined with an artificial intelligence (AI) algorithm. To identify the minor and occasional inconsistencies in strabismus patients during the binocular coordination process, which are usually seen in early-stage patients and rarely recognized in current studies, the system captures temporally and spatially continuous high-definition infrared images of the eye during wide-angle continuous motion, and is effective in inducing intermittent strabismus. Based on the collected eye motion information, 16 features of the oculomotor process with strong physiological interpretations, which help biomedical staff understand and evaluate results generated later, are calculated through the introduction of pupil-canthus vectors. These features can be normalized, and reflect individual differences. After these features are processed by the random forest (RF) algorithm, this method experimentally yields 97.1% accuracy in strabismus detection in 70 people under diverse indoor testing conditions, validating the high accuracy and robustness of the method, and implying that the method has strong potential to support widespread and highly accurate strabismus screening.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11852461/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parallel Detection of the Unamplified Carbapenem Resistance Genes bla_NDM-1 and bla_OXA-1 Using a Plasmonic Nano-Biosensor with a Field-Portable DNA Extraction Method.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-14 DOI: 10.3390/bios15020112

Kaily Kao, Evangelyn C Alocilja

{"title":"Parallel Detection of the Unamplified Carbapenem Resistance Genes blaNDM-1 and blaOXA-1 Using a Plasmonic Nano-Biosensor with a Field-Portable DNA Extraction Method.","authors":"Kaily Kao, Evangelyn C Alocilja","doi":"10.3390/bios15020112","DOIUrl":"10.3390/bios15020112","url":null,"abstract":"Antimicrobial resistance (AMR) is a rapidly growing global concern resulting from the overuse of antibiotics in agricultural and clinical settings. The challenge is exacerbated by the lack of rapid surveillance for resistant bacteria in clinical, environmental, and food supply settings. The increasing resistance to carbapenems, an important sub-class of beta-lactam antibiotics, is a major concern in the healthcare community. Carbapenem resistance (CR) has been found in the environment and food supply chain, where it has the potential to spread to pathogens, animals, and humans through direct or indirect contact. Rapid detection for preventative and control measures should be developed. This study utilized a gold nanoparticle-based plasmonic biosensor for the parallel detection of the CR genes blaNDM-1 and blaOXA-1. To explore the field portability, DNA was extracted using two methods: a commercial extraction kit and a boiling method. The results were compared between the two methods using a spectrophotometer and a cellphone application for RGB values to quantify the visual results. The results showed that the boiling method of extraction was more effective than extraction with a commercial kit for this analysis. The parallel detection of unamplified genes extracted via the boiling method is novel. When combined with other portable testing equipment, the approach has the potential to be an inexpensive, rapid, and simple on-site CR gene detection protocol.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11853256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioluminescent Microbial Bioreporters: A Personal Perspective.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-14 DOI: 10.3390/bios15020111

Shimshon Belkin

{"title":"Bioluminescent Microbial Bioreporters: A Personal Perspective.","authors":"Shimshon Belkin","doi":"10.3390/bios15020111","DOIUrl":"10.3390/bios15020111","url":null,"abstract":"This review attempts to summarize my three decades-long involvement in, and contribution to, the design, construction and testing of bioluminescent microbial sensor strains (bioreporters). With the understanding that such a document cannot be completely free of bias, the review focuses on studies from my own lab only, with almost no coverage of the parallel progress made by others in similar fields. This admittedly subjective approach by no way detracts from the achievements of countless excellent researchers who are not mentioned here, and whose contributions to the field are at least as important as that of my own. The review covers basic aspects of microbial sensor design, and then progresses to describe approaches to performance improvement of sensor strains aimed at the detection of either specific chemicals, groups of chemicals sharing similar characteristics, or global effects, such as toxicity and genotoxicity. The need for integration of live sensor cells into a compatible hardware platform is highlighted, as is the importance of long-term maintenance of the cells' viability and activity. The use of multi-member sensors' panels is presented as a means for enhancing the detection spectrum and sample \"fingerprinting\", along with a list of different purposes to which such sensors have been put to use.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11853290/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Equivalent Circuit Modeling and Analysis for Microfluidic Electrical Impedance Monitoring of Single-Cell Growth. 单细胞生长微流体电阻抗监测的等效电路建模与分析

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-14 DOI: 10.3390/bios15020113

Yingying Wang, Haoran Wu, Yulu Geng, Zhao Zhang, Jiaming Fu, Jia Ouyang, Zhen Zhu

{"title":"Equivalent Circuit Modeling and Analysis for Microfluidic Electrical Impedance Monitoring of Single-Cell Growth.","authors":"Yingying Wang, Haoran Wu, Yulu Geng, Zhao Zhang, Jiaming Fu, Jia Ouyang, Zhen Zhu","doi":"10.3390/bios15020113","DOIUrl":"10.3390/bios15020113","url":null,"abstract":"Microfluidics has significantly advanced the field of single-cell analysis, particularly in studies related to cell growth, division, and heterogeneity. Electrical impedance spectroscopy (EIS), a label-free and non-invasive biosensing technique, has been integrated into microfluidic devices for high-throughput and long-term monitoring of single budding yeast cells. Accurate interpretation of EIS measurements of cell growth dynamics necessitates the establishment of theoretical equivalent circuit models for the single-cell sensing system. Here, we report on the development of equivalent circuit models of an in situ EIS sensing system to elucidate cell growth. Firstly, finite element modeling and simulation of an EIS measurement of cell growth in the EIS sensing unit were performed, guiding the fittings of electrical components for an established equivalent circuit model (ECM). From the ECM, we extracted an equivalent volume fraction applicable to various cell and sensing unit geometries to describe the geometry-dependent sensing characteristics corresponding to the electrical response in the model. Then, EIS measurements of an immobilized cell in a microfluidic device were conducted via peripheral circuits. A lumped parameter model for the entire EIS measurement system was established, with electrical components determined by fitting to experimental data. The rationality of the proposed theoretical model was validated through the long-term impedance variation induced by cell growth in experiments, demonstrating its feasibility in linking EIS data with the bio-physics underlying the experimental phenomenon.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11853229/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Embroidered Electrochemical Sensor to Measure Glucose Made with Commercially Available Textile Materials.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-14 DOI: 10.3390/bios15020109

Marc Martínez-Estrada, Ignacio Gil, Raúl Fernández-García

{"title":"An Embroidered Electrochemical Sensor to Measure Glucose Made with Commercially Available Textile Materials.","authors":"Marc Martínez-Estrada, Ignacio Gil, Raúl Fernández-García","doi":"10.3390/bios15020109","DOIUrl":"10.3390/bios15020109","url":null,"abstract":"A textile electrochemical sensor manufactured with commercially available textile materials is presented to determine glucose concentration. The sensor design consists of three electrodes manufactured with two different conductive yarns, one made with a silver coating and the other with stainless steel fibres. Different combinations of them are used to prepare three different electrochemical textile sensor combinations. The first sensor is built only with silver-coated yarn and used as a reference sensor. The other two sensors are prepared with different combinations of conductive yarns. The textile sensors perform a cyclic voltammetric test, where it is demonstrated that the glucose concentration over the sensor can be related with the increase in the current measured. The results allow us to identify feeding voltages where the concentration-current relation is close to linear. The textile sensor shows a sensitivity between 0.0145 and 0.0452 μA/(mg/dL) for the 45-180 mg/dL glucose concentration range and 0.0012 and 0.0035 μA/(mg/dL) for the 180-1800 mg/dL range for the different sensor types presented. The regression coefficients for the sensitivities range between 0.9266 and 0.9954. This research demonstrates the feasibility to develop a fully integrated textile electrochemical sensor made completely with commercially available textile materials.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11853155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0