Biosensors-Basel最新文献_第10页

Development of a Mass-Producible Microfluidic Device for Single and Bulk Mycobacteria Investigations.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-13 DOI: 10.3390/bios15020108

Adrian J T Teo, Jianhui Gu, Alexander Govyadinov, Pavel Kornilovitch, Peiyun Wang, Serene Goh, Nguyen Truong Tung, Zhen Peng, Keith Koh, King Ho Holden Li

引用次数: 0

Recent Advances in MXene-Based Electrochemical Sensors.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-13 DOI: 10.3390/bios15020107

Ziyi Zhao, Jiayi Cao, Boyu Zhu, Xinru Li, Lin Zhou, Bin Su

{"title":"Recent Advances in MXene-Based Electrochemical Sensors.","authors":"Ziyi Zhao, Jiayi Cao, Boyu Zhu, Xinru Li, Lin Zhou, Bin Su","doi":"10.3390/bios15020107","DOIUrl":"10.3390/bios15020107","url":null,"abstract":"MXene is a new family of two-dimensional nanomaterials with outstanding electrical conductivity, tunable structure, biocompatibility, and a large surface area. Thanks to these unique physicochemical properties, MXene has been used for constructing electrochemical sensors (MECSens) with excellent performance. In particular, the abundant surface termination of MXene can contribute to greatly enhancing the analytical sensitivity and selectivity of MECSens. Recently, MECSens have been widely applied in many fields including clinical diagnosis, infectious disease surveillance, and food security. However, not all MXene materials are suitable for building electrochemical sensors. In this article, we present an overview of different MECSens that have been developed so far. We begin with a short summary of the preparation and characterization of MECSens. Subsequently, the electrochemical performance, detection strategies, and application scenarios of MECSens are classified and briefly discussed. The article ends with a short conclusion and future perspectives. We hope this article will be helpful for designing and constructing MECSens with outstanding activity for electrochemical analysis.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11852424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Biomarkers for Malaria: Advances in Early Detection and Asymptomatic Diagnosis. 探索疟疾生物标志物：早期检测和无症状诊断的进展。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-12 DOI: 10.3390/bios15020106

Jacko Abiwaqash Harmonis, Sri Agung Fitri Kusuma, Yaya Rukayadi, Aliya Nur Hasanah

{"title":"Exploring Biomarkers for Malaria: Advances in Early Detection and Asymptomatic Diagnosis.","authors":"Jacko Abiwaqash Harmonis, Sri Agung Fitri Kusuma, Yaya Rukayadi, Aliya Nur Hasanah","doi":"10.3390/bios15020106","DOIUrl":"10.3390/bios15020106","url":null,"abstract":"Malaria is a tropical disease caused by the Plasmodium parasite, which was responsible for 249 million cases worldwide in 2022. Malaria is currently diagnosed using RDTs, PCR-based methods, or blood smear microscopy. Ideal biomarkers have been identified for malaria, with the potential for improving treatment, diagnosis, and overall clinical outcomes. This review discusses the types of existing biomarkers and the opportunities for new biomarkers to be used as diagnostic components in detecting Plasmodium, including in terms of sensitivity, detection limit, specificity, and the species of Plasmodium that can be detected. Following a comparison, five main ideal malaria biomarkers were identified, namely HRP2, pLDH, hemozoin, aldolase, and pGDH. These biomarkers distinguished themselves markedly from the others in terms of specificity in Plasmodium detection, sensitivity in analysis, and the use of non-invasive samples. Several other biomarkers, such as CRP, Ang-1, Ang-2, and PCT, show potential for malaria detection in terms of their ability to differentiate disease severity, and the levels of these biomarkers can be determined in the body for comparison with malaria parasitemia. Of the five ideal biomarkers, hemozoin and aldolase can still be developed regarding the types of samples used and their sensitivity to different Plasmodium species. Further research on the biomarkers CRP, Ang-1, Ang-2, and PCT is still needed to evaluate their potential.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11853453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomass-Derived Carbon Dots as Fluorescent Probes for Label-Free Sensing of Hemin and as Radical Scavengers.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-12 DOI: 10.3390/bios15020105

Neha Sharma, Hae-Jeung Lee

{"title":"Biomass-Derived Carbon Dots as Fluorescent Probes for Label-Free Sensing of Hemin and as Radical Scavengers.","authors":"Neha Sharma, Hae-Jeung Lee","doi":"10.3390/bios15020105","DOIUrl":"10.3390/bios15020105","url":null,"abstract":"Carbon dots (CDs) derived from biomass are promising fluorescent probes for specific analyte detection due to their specificity, biocompatibility, selectivity, and sensitivity. In this work, carbon dots were prepared hydrothermally from natural material, Myrica esculenta fruits (hereafter referred to as MPCDs), without adding any chemicals. The prepared MPCDs were characterized using optical, microscopic, and spectroscopic methods that revealed the presence of numerous functional groups and fluorescent properties. MPCDs exhibited exceptional characteristics such as water solubility, photostability, excitation-dependent fluorescence emission, and ionic stability. Transmission electron microscopy found that the average size of the MPCDs was 8 nm. MPCDs exhibited remarkable sensing ability for hemin, with a good linearity (R2 = 0.999) and a lower limit of detection of 14.1 nM. MPCDs demonstrated fluorescence quenching-based detection of hemin, primarily owing to ground state complex formation and the inner filter effect. Furthermore, the prepared material exhibited excellent antioxidant potential against 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) and 2,2-diphenyl-1-picrylhydrazyl radicals with EC50 values of 25.4 and 205.4 µg/mL, respectively. The study suggests that CDs from Myrica esculenta fruits could be used as optical sensors for hemin detection as well as to scavenge selected radicals.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11853633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Microfluidic Ion-Exchange Optode System for Point-of-Care Determination of Sodium Concentration in Serum.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-12 DOI: 10.3390/bios15020104

Kuan-Hsun Huang, Cheng-Xue Yu, Chia-Chun Lee, Chin-Chung Tseng, Lung-Ming Fu

{"title":"Rapid Microfluidic Ion-Exchange Optode System for Point-of-Care Determination of Sodium Concentration in Serum.","authors":"Kuan-Hsun Huang, Cheng-Xue Yu, Chia-Chun Lee, Chin-Chung Tseng, Lung-Ming Fu","doi":"10.3390/bios15020104","DOIUrl":"10.3390/bios15020104","url":null,"abstract":"A microfluidic system for detecting sodium ions (Na+) has been developed, incorporating a micro finger-pump chip and a micro-spectrometer platform to measure Na+ concentration in human serum. A small volume (10 μL) of serum sample is introduced into the microchip and reacted with a preloaded reagent mixture through a two-step finger-pump actuation process. The resulting purple complex is directed into the detection area of the chip and analyzed using the micro-spectrometer at wavelengths of 555 and 666 nm. The Na+ concentration is then inversely derived from the measured A555/A666 absorbance ratio using self-written software installed on a Raspberry Pi. The entire detection process is completed in just 3 min, offering a significant advantage in meeting clinical needs compared to the traditional reporting turnaround time of several hours in medical institutions. The experimental results indicate a linear relationship between the measured absorbance ratio and Na+ concentration within the range of 1-200 mM, with a correlation coefficient of R2 = 0.9989. Additionally, the detection results from 60 serum samples collected from chronic kidney disease (CKD) patients showed a strong agreement with those obtained using the conventional indirect ion-selective electrode (ISE) method, achieving a correlation coefficient of R2 = 0.9885 and an average recovery rate of 99.4%. In summary, the proposed system provides a practical, affordable, and rapid alternative to conventional Na+ detection methods, making it highly promising for point-of-care (POC) testing applications.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11853684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NanoBioAnalytical (NBA) Platform to Decipher Extracellular Vesicles Secreted by Microvascular Endothelial Cells Under Benzo[a]pyrene Exposure. 利用纳米生物分析 (NBA) 平台解读微血管内皮细胞在苯并[a]芘暴露下分泌的胞外小泡。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-11 DOI: 10.3390/bios15020103

Geetika Raizada, Joan Guillouzouic, Alain Rouleau, Eric Lesniewska, Eric Le Ferrec, Céline Elie-Caille, Wilfrid Boireau

{"title":"NanoBioAnalytical (NBA) Platform to Decipher Extracellular Vesicles Secreted by Microvascular Endothelial Cells Under Benzo[a]pyrene Exposure.","authors":"Geetika Raizada, Joan Guillouzouic, Alain Rouleau, Eric Lesniewska, Eric Le Ferrec, Céline Elie-Caille, Wilfrid Boireau","doi":"10.3390/bios15020103","DOIUrl":"10.3390/bios15020103","url":null,"abstract":"Recent advances in the clinical extracellular vesicles (EVs) field highlight their potential as biomarkers for diverse diseases and therapeutic applications. This study provides an in-depth characterization of 10k EVs from human microvascular endothelial cells (HMEC-1) exposed to benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon found in food and smoke. Given EVs' complexity, with numerous surface and cargo proteins, phenotyping remains challenging. Here, we introduce a multiplex biosensor, in µarray format, for profiling EVs from distinct cellular conditions, employing a multimodal approach that combines surface plasmon resonance imaging (SPRi) and in situ atomic force microscopy (AFM) to decipher EVs' biochemical and biophysical properties. SPRi experiments showed notable EV capture differences on ligands such as Anti-CD36, Anti-CD81, and Anti-ApoA between treated and control conditions, likely due to B[a]P exposure. A complementary AFM study and statistical analyses revealed size differences between EVs from treated and control samples, with ligands like Annexin-V, Anti-CD36, and Anti-VEGFR1 emerging as ligands specific to potential cytotoxicity biomarkers. Our findings suggest that B[a]P exposure may increase EV size and alter marker expression, indicating phenotypic shifts in EVs under cytotoxic stress. The original combination of SPRi and AFM reveals valuable data on the phenotypical and morphological heterogeneities of EV subsets linked to cytotoxic stresses and highlights the potential of EVs as specific toxicological markers.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11852858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

(Bio)Electroanalysis of Tetracyclines: Recent Developments.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-11 DOI: 10.3390/bios15020101

Maria Madej, Paweł Knihnicki, Radosław Porada, Jolanta Kochana

引用次数: 0

Development of Neurodegenerative Disease Diagnosis and Monitoring from Traditional to Digital Biomarkers. 从传统生物标记物到数字生物标记物的神经退行性疾病诊断和监测的发展。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-11 DOI: 10.3390/bios15020102

Jaeyoon Song, Eunseo Cho, Huiseop Lee, Suyoung Lee, Sehyeon Kim, Jinsik Kim

引用次数: 0

Recent Progress in Flexible Microelectrode Arrays for Combined Electrophysiological and Electrochemical Sensing.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-10 DOI: 10.3390/bios15020100

Umisha Siwakoti, Steven A Jones, Deepak Kumbhare, Xinyan Tracy Cui, Elisa Castagnola

{"title":"Recent Progress in Flexible Microelectrode Arrays for Combined Electrophysiological and Electrochemical Sensing.","authors":"Umisha Siwakoti, Steven A Jones, Deepak Kumbhare, Xinyan Tracy Cui, Elisa Castagnola","doi":"10.3390/bios15020100","DOIUrl":"10.3390/bios15020100","url":null,"abstract":"Understanding brain function requires advanced neural probes to monitor electrical and chemical signaling across multiple timescales and brain regions. Microelectrode arrays (MEAs) are widely used to record neurophysiological activity across various depths and brain regions, providing single-unit resolution for extended periods. Recent advancements in flexible MEAs, built on micrometer-thick polymer substrates, have improved integration with brain tissue by mimicking the brain's soft nature, reducing mechanical trauma and inflammation. These flexible, subcellular-scale MEAs can record stable neural signals for months, making them ideal for long-term studies. In addition to electrical recording, MEAs have been functionalized for electrochemical neurotransmitter detection. Electroactive neurotransmitters, such as dopamine, serotonin, and adenosine, can be directly measured via electrochemical methods, particularly on carbon-based surfaces. For non-electroactive neurotransmitters like acetylcholine, glutamate, and γ-aminobutyric acid, alternative strategies, such as enzyme immobilization and aptamer-based recognition, are employed to generate electrochemical signals. This review highlights recent developments in flexible MEA fabrication and functionalization to achieve both electrochemical and electrophysiological recordings, minimizing sensor fowling and brain damage when implanted long-term. It covers multi-time scale neurotransmitter detection, development of conducting polymer and nanomaterial composite coatings to enhance sensitivity, incorporation of enzyme and aptamer-based recognition methods, and the integration of carbon electrodes on flexible MEAs. Finally, it summarizes strategies to acquire electrochemical and electrophysiological measurements from the same device.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11853293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon Quantum Dots: Synthesis, Characteristics, and Quenching as Biocompatible Fluorescent Probes.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-10 DOI: 10.3390/bios15020099

Arif Kamal, Seongin Hong, Heongkyu Ju

引用次数: 0