Biosensors-Basel最新文献_第10页

Gait Phase Recognition in Multi-Task Scenarios Based on sEMG Signals. 基于表面肌电信号的多任务场景步态相位识别。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-10 DOI: 10.3390/bios15050305

Xin Shi, Xiaheng Zhang, Pengjie Qin, Liangwen Huang, Yaqin Zhu, Zixiang Yang

{"title":"Gait Phase Recognition in Multi-Task Scenarios Based on sEMG Signals.","authors":"Xin Shi, Xiaheng Zhang, Pengjie Qin, Liangwen Huang, Yaqin Zhu, Zixiang Yang","doi":"10.3390/bios15050305","DOIUrl":"10.3390/bios15050305","url":null,"abstract":"In the human-exoskeleton interaction process, accurately recognizing gait phases is crucial for effectively assessing the assistance provided by the exoskeleton. However, due to the similarity in muscle activation patterns between adjacent gait phases, the recognition accuracy is often low, which can easily lead to confusion in surface electromyography (sEMG) feature extraction. This paper proposes a real-time recognition method based on multi-scale fuzzy approximate root mean entropy (MFAREn) and an Efficient Multi-Scale Attention Convolutional Neural Network (EMACNN), building upon the concept of fuzzy approximate entropy. MFAREn is used to extract the dynamic complexity and energy intensity features of sEMG signals, serving as the input matrix for EMACNN to achieve fast and accurate gait phase recognition. This study collected sEMG signals from 10 subjects performing continuous lower limb gait movements in five common motion scenarios for experimental validation. The results show that the proposed method achieves an average recognition accuracy of 95.72%, outperforming the other comparison methods. The method proposed in this paper is significantly different compared to other methods (p < 0.001). Notably, the recognition accuracy for walking in level walking, stairs ascending, and ramp ascending exceeds 95.5%. This method demonstrates a high recognition accuracy, enabling sEMG-based gait phase recognition and meeting the requirements for effective human-exoskeleton interaction.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-Parametric Electrochemical Sensing Platform: Applications in Animal Welfare. 多参数电化学传感平台：在动物福利中的应用。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-10 DOI: 10.3390/bios15050304

C Ferreira, E Lynch, A O'Herlihy, F Barry, L C Nagle, S R Teixeira, P Galvin

{"title":"Multi-Parametric Electrochemical Sensing Platform: Applications in Animal Welfare.","authors":"C Ferreira, E Lynch, A O'Herlihy, F Barry, L C Nagle, S R Teixeira, P Galvin","doi":"10.3390/bios15050304","DOIUrl":"10.3390/bios15050304","url":null,"abstract":"The rapid growth of the dairy sector requires advanced monitoring tools to ensure sustainable practices that benefit the environment, economy, and human health. Current monitoring devices often lack multi-parametric capabilities, limiting their ability to provide comprehensive data on critical chemical and biochemical parameters. To address this challenge, this work presented the integration of a real-time multi-parametric device with sensors for pH, temperature, nitrate, and nitrite, providing a comprehensive solution to dairy cattle health monitoring. This solution included an electrochemical platform, Portable Unit for Lab-on-Site Electrochemistry (PULSE), and an application for data processing and display. In-house fabricated flexible gold-printed electrodes demonstrated accurate detection of nitrite and nitrate when integrated with the PULSE, achieving sensitivities of 6.32 μA/ppm/cm2 in artificial interstitial fluid and 1.92 μA/ppm/cm2 in phosphate buffered saline, respectively. The PULSE achieved 65.83% and 58.3% lower limits of detection in phosphate buffered saline than a benchtop potentiostat, for nitrate and nitrite, respectively, along with a 24.5% increase in nitrite sensitivity, enhancing its ability to detect lower analyte concentrations. pH sensing was carried out with a commercial screen-printed electrode coated with a layer of iridium oxide. The pH was tested in ruminal complex fluid, obtaining a pH sensitivity of -59.63 mV/pH and an accuracy of 98.9%. These findings highlighted the potential of this technology as an effective tool for dairy cattle health monitoring and its deployment in real-world scenarios.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AFM-Based Monitoring of Enzymatic Activity of Individual Molecules of Cytochrome CYP102A1. 基于afm的细胞色素CYP102A1单个分子酶活性监测。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-10 DOI: 10.3390/bios15050303

Yuri D Ivanov, Natalia S Bukharina, Ivan D Shumov, Oleg N Afonin, Vadim Y Tatur, Anna V Grudo, Alexander I Archakov

{"title":"AFM-Based Monitoring of Enzymatic Activity of Individual Molecules of Cytochrome CYP102A1.","authors":"Yuri D Ivanov, Natalia S Bukharina, Ivan D Shumov, Oleg N Afonin, Vadim Y Tatur, Anna V Grudo, Alexander I Archakov","doi":"10.3390/bios15050303","DOIUrl":"10.3390/bios15050303","url":null,"abstract":"Herein, we report the use of a nanotechnology-based approach for the study of enzyme-functionalized mica surfaces. Atomic force microscopy (AFM) has been employed for the determination of the catalytic activity of single molecules of heme-containing cytochrome P450 CYP102A1 (CYP102A1) enzyme, which was immobilized on the surface of a mica chip. Height fluctuations in individual molecules of the enzyme were measured under near-native conditions by AFM measurements in liquid using a cantilever with a 10 to 20 nm tip curvature radius. We have found that in the process of enzymatic catalysis, the mean amplitude of height fluctuations in individual enzyme molecules is 1.4-fold higher than that of enzyme molecules in an inactive state. The temperature dependence of the mean amplitude of height fluctuations in cytochrome CYP102A1 has been revealed, and the maximum of this dependence has been observed at 22 °C. The proposed nanotechnology-based approach can be employed in studies of a wide variety of enzymes, which are important for the development of novel diagnostic tests and systems for pharmaceutical analysis. The approach developed in our work will favor further miniaturization of enzyme-based biosensors and the transition from traditional sensors to nanobiosensors.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bridging Blood and Skin: Biomarker Profiling in Dermal Interstitial Fluid (dISF) for Minimally Invasive Diagnostics. 桥接血液和皮肤：真皮间质液（dISF）的生物标志物分析用于微创诊断。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-09 DOI: 10.3390/bios15050301

Yann Sprunger, Johan Longo, Ali Saeidi, Adrian M Ionescu

{"title":"Bridging Blood and Skin: Biomarker Profiling in Dermal Interstitial Fluid (dISF) for Minimally Invasive Diagnostics.","authors":"Yann Sprunger, Johan Longo, Ali Saeidi, Adrian M Ionescu","doi":"10.3390/bios15050301","DOIUrl":"10.3390/bios15050301","url":null,"abstract":"Understanding the biochemical relationship between serum and dermal interstitial fluid (dISF) is critical for advancing minimally invasive diagnostics with wearables and point of care devices focusing on most relevant biomarkers accessible in the ISF. This work compares the composition of dISF and serum using Xsensio's microneedle-based collector, which yields an average of 3.4 μL/h. In the first study, total protein content, human serum albumin (HSA), and immunoglobulin G (IgG) are quantified in twelve volunteers. A second study is dedicated to screening 50 inflammation-related protein biomarkers across twenty volunteers. The results demonstrate that dISF closely resembles serum in its major protein constituents but at reduced concentrations (e.g., 57% for total protein). Strong correlations are observed between dISF and serum for CRP and SAA (R2>0.87), primarily driven by a subject with pathological levels, demonstrating the ability of dISF to reflect systemic inflammation. This study originally reports NT-proBNP detection at comparable levels in both fluids, suggesting that dISF could serve as a reliable proxy for blood NT-proBNP in the non-invasive diagnosis of cardiac failure. Cytokine profiling reveals 36 detectable cytokines, including several unique to dISF. Notably, interleukin concentrations are found to be highly similar between the two fluids. These experimental findings support dISF as a promising diagnostic medium for monitoring both localized and systemic biomarkers in clinical applications.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12110442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Electrochemical Aptasensor for Accurate and Sensitive Detection of Exosomes Based on Dual-Probe Recognition and Hybridization Chain Reaction. 基于双探针识别和杂交链反应的准确灵敏检测外泌体的电化学配体传感器。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-09 DOI: 10.3390/bios15050302

Haojie Ma, Jie Li, Mengjia Gao, Yan Dong, Yi Luo, Shao Su

{"title":"An Electrochemical Aptasensor for Accurate and Sensitive Detection of Exosomes Based on Dual-Probe Recognition and Hybridization Chain Reaction.","authors":"Haojie Ma, Jie Li, Mengjia Gao, Yan Dong, Yi Luo, Shao Su","doi":"10.3390/bios15050302","DOIUrl":"10.3390/bios15050302","url":null,"abstract":"The accurate and sensitive detection of tumor-derived exosomes holds significant promise for the early diagnosis of cancer. In this study, an electrochemical aptasensor was developed for the high-performance detection of exosomes by integrating dual-probe recognition and hybridization chain reaction (HCR). A dual-probe recognition unit composed of a MUC1 aptamer (MUC1-Apt) probe and cholesterol probe was designed for capturing target exosomes and reducing the interference from free proteins, significantly improving the accuracy of exosome detection. It should be noted that the dual-probe recognition unit was formed in conjunction with the HCR. Moreover, a large number of biotins were also assembled on the HCR product, which were used to capture avidin-horseradish peroxidase (SA-HRP) for signal amplification. The CD63 aptamer (CD63-Apt) was immobilized on the surface of a gold electrode for specifically capturing exosomes to construct a classical sandwiched structure. The loaded SA-HRP can efficiently catalyze the reaction of 3, 3', 5, 5' tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) to generate a large electrochemical signal. According to this phenomenon, a linear relationship of this proposed aptasensor was achieved between the electrochemical response and 1 × 102-1 × 107 particles/mL exosomes, with a detection limit of 45 particles/mL. Moreover, the aptasensor exhibited accepted stability and potential clinical applicability. All results proved that this aptasensor has a promising application in exosome-based disease diagnostics.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109912/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Association Assay Systems for Imaging Protein-Protein Interactions in Mammalian Cells. 成像蛋白质-哺乳动物细胞中蛋白质相互作用的分子关联分析系统。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-08 DOI: 10.3390/bios15050299

Sung-Bae Kim, Tadaomi Furuta, Suresh Thangudu, Arutselvan Natarajan, Ramasamy Paulmurugan

{"title":"Molecular Association Assay Systems for Imaging Protein-Protein Interactions in Mammalian Cells.","authors":"Sung-Bae Kim, Tadaomi Furuta, Suresh Thangudu, Arutselvan Natarajan, Ramasamy Paulmurugan","doi":"10.3390/bios15050299","DOIUrl":"10.3390/bios15050299","url":null,"abstract":"Molecular imaging probes play a pivotal role in assaying molecular events in various physiological systems. In this study, we demonstrate a new genre of bioluminescent probes for imaging protein-protein interactions (PPIs) in mammalian cells, named the molecular association assay (MAA) probe. The MAA probe is designed to be as simple as a full-length marine luciferase fused to a protein of interest with a flexible linker. This simple fusion protein alone surprisingly works by recognizing a specific ligand, interacting with a counterpart protein of the PPI, and developing bioluminescence (BL) in mammalian cells. We made use of an artificial intelligence (AI) tool to simulate the binding modes and working mechanisms. Our AlphaFold-based analysis on the binding mode suggests that the hinge region of the MAA probe is flexible before ligand binding but becomes stiff after ligand binding and protein association. The sensorial properties of representative MAA probes, FRB-ALuc23 and FRB-R86SG, are characterized with respect to the quantitative feature, BL spectrum, and in vivo tumor imaging using xenografted mice. Our AI-based simulation of the working mechanisms reveals that the association of MAA probes with the other proteins works in a way to facilitate the substrate's access to the active sites of the luciferase (ALuc23 or R86SG). We prove that the concept of MAA is generally applicable to other examples, such as the ALuc16- or R86SG-fused estrogen receptor ligand-binding domain (ER LBD). Considering the versatility of this conceptionally unique and distinctive molecular imaging probe compared to conventional ones, we are expecting the widespread application of these probes as a new imaging repertoire to determine PPIs in living organisms.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109908/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing Whole-Cell Biosensors for the Early Detection of Crop Infections: A Proof-of-Concept Study. 优化全细胞生物传感器用于作物感染的早期检测：概念验证研究。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-08 DOI: 10.3390/bios15050300

Nadav Zanger, Evgeni Eltzov

引用次数: 0

Centrifugation-Induced Stable Colloidal Silver Nanoparticle Aggregates for Reproducible Surface-Enhanced Raman Scattering Detection. 离心诱导稳定胶体银纳米粒子聚集体用于可重复表面增强拉曼散射检测。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-08 DOI: 10.3390/bios15050298

Tianyu Zhou, Zhiyang Zhang

{"title":"Centrifugation-Induced Stable Colloidal Silver Nanoparticle Aggregates for Reproducible Surface-Enhanced Raman Scattering Detection.","authors":"Tianyu Zhou, Zhiyang Zhang","doi":"10.3390/bios15050298","DOIUrl":"10.3390/bios15050298","url":null,"abstract":"Colloidal noble metal nanoparticle aggregates have demonstrated significant advantages in surface-enhanced Raman scattering (SERS) analysis, particularly for online detection, due to their excellent optical properties, spatial homogeneity, and fluidic compatibility. However, conventional chemically induced aggregation methods (such as salt-induced nanoparticle aggregation) suffer from uncontrolled aggregation, limited stability, and narrow detection windows, which restrict their quantitative and long-term applications. In this study, we developed a non-chemical method for fabricating stable colloidal aggregates from uniform β-cyclodextrin-stabilized silver nanoparticles (β-CD@AgNPs) via centrifugation. By precisely controlling the addition rate of silver nitrate, we synthesized β-cyclodextrin-stabilized silver nanoparticles with a uniform size. Surprisingly, these nanoparticles can form highly dispersed and homogeneous colloidal aggregates simply via centrifugation, which is completely different from the behavior of traditional ligand-modified nanoparticles. Notably, the resulting aggregates exhibit excellent SERS enhancement, enabling the sensitive detection of various dyes at nanomolar levels. Furthermore, they maintain a stable SERS signal (RSD = 6.99%) over a detection window exceeding 1 h, markedly improving signal stability and reproducibility compared with salt-induced aggregates. Additionally, using pyocyanin as a model analyte, we evaluated the quantitative performance of these aggregates (LOD = 0.2 nM), achieving satisfactory recovery (82-117%) in spiked samples of drinking water, lake water, and tap water. This study provides a facile strategy for fabricating stable colloidal SERS substrates and paves the way for the advancement of SERS applications in analytical sciences.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12110687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Review of Non-Invasive Imaging Technologies for Cutaneous Melanoma. 皮肤黑色素瘤无创成像技术综述。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-07 DOI: 10.3390/bios15050297

Luke Horton, Joseph W Fakhoury, Rayyan Manwar, Ali Rajabi-Estarabadi, Dilara Turk, Sean O'Leary, Audrey Fotouhi, Steven Daveluy, Manu Jain, Keyvan Nouri, Darius Mehregan, Kamran Avanaki

引用次数: 0

Carbon Nanotube-Based Field-Effect Transistor Biosensors for Biomedical Applications: Decadal Developments and Advancements (2016-2025). 生物医学应用的碳纳米管场效应晶体管生物传感器：十年发展和进步（2016-2025）。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-07 DOI: 10.3390/bios15050296

Joydip Sengupta, Chaudhery Mustansar Hussain

引用次数: 0