Biosensors-Basel最新文献_第10页

Correction: Bossi et al. Time-Resolved Fluorescence Spectroscopy of Molecularly Imprinted Nanoprobes as an Ultralow Detection Nanosensing Tool for Protein Contaminants. Biosensors 2023, 13, 745. 更正：Bossi等人。分子印迹纳米探针的时间分辨荧光光谱作为蛋白质污染物的超低检测纳米传感工具。生物医学工程学报，2016,33(4):745 - 745。

IF 5.6 3区工程技术

Biosensors-Basel Pub Date : 2026-02-28 DOI: 10.3390/bios16030141

Alessandra Maria Bossi, Alice Marinangeli, Alberto Quaranta, Lucio Pancheri, Devid Maniglio

引用次数: 0

The Synthesis of Ru-Co-Oxalate MOFs for an Electrochemiluminescent Glyphosate Sensor. 电化学发光草甘膦传感器用ru - co -草酸mof的合成。

IF 5.6 3区工程技术

Biosensors-Basel Pub Date : 2026-02-28 DOI: 10.3390/bios16030140

Karina G Espinosa-Cavazos, Joelis Rodríguez-Hernández, Carlos Gallardo-Vega, Carmen Alvarado-Canché, Marco Antonio Castillo, Roman Torres-Lubian, Perla E García Casillas, Juan Carlos Anaya-Zavaleta, Antonio Ledezma-Pérez, Arxel de León

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Duplex Recombinase-Aided Amplification-Lateral Flow Dipstick (dRAA-LFD) Assay for New Zealand Green-Lipped Mussel Authentication. 双重组酶辅助扩增-横向流动量尺（dRAA-LFD）检测新西兰绿唇贻贝鉴定。

IF 5.6 3区工程技术

Biosensors-Basel Pub Date : 2026-02-27 DOI: 10.3390/bios16030138

Jirakrit Saetang, Maturada Saengthong, Soottawat Benjakul, Gururaj Moorthy

{"title":"Duplex Recombinase-Aided Amplification-Lateral Flow Dipstick (dRAA-LFD) Assay for New Zealand Green-Lipped Mussel Authentication.","authors":"Jirakrit Saetang, Maturada Saengthong, Soottawat Benjakul, Gururaj Moorthy","doi":"10.3390/bios16030138","DOIUrl":"10.3390/bios16030138","url":null,"abstract":"New Zealand green-lipped mussel (Perna canaliculus) is a premium seafood product that may be substituted with morphologically similar mussels after shucking and cooking, particularly Asian green mussel (Perna viridis). This study developed a rapid, on-site duplex recombinase-aided amplification-lateral flow dipstick (dRAA-LFD) assay to authenticate P. canaliculus and differentiate it from P. viridis. Species-specific primers were designed from mitochondrial COI alignment and combined in a dRAA reaction. Reaction conditions were optimized at 37-42 °C and 15-60 min. Specificity was assessed against 11 non-target seafood species, and sensitivity was evaluated using 2-fold serial dilutions. The assay was further validated using DNA from boiled (85 °C, 5-15 min), steamed (105 °C, 10-30 min), and fried (185 °C, 30-90 s) mussels, and 15 restaurant products labeled as New Zealand mussel dishes. Optimal performance was achieved at 40 °C for 30 min, with no cross-reactivity. The LFD detection limits were 0.05 ng/reaction for P. viridis and 0.2-0.1 ng/reaction for P. canaliculus. All cooked samples remained identifiable, and commercial testing classified 13/15 products as P. canaliculus and 2/15 as P. viridis. Overall, the dRAA-LFD assay enables rapid, equipment-light authentication of cooked mussel products for routine screening.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"16 3","pages":""},"PeriodicalIF":5.6,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13023652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147533743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Identification of Trace Pharmacodynamic Substances in Traditional Chinese Medicine via SERS and Deep Learning. 基于SERS和深度学习的中药中痕量药效学物质的快速鉴定。

IF 5.6 3区工程技术

Biosensors-Basel Pub Date : 2026-02-27 DOI: 10.3390/bios16030139

Huixuan Yang, Mingyuan Chen, Jiayi Wang, Xufei Tong, Huiru Li, Hao Chen, Zengshan Yu, Chunying Zhao, Mingli Wang, Guochao Shi

{"title":"Rapid Identification of Trace Pharmacodynamic Substances in Traditional Chinese Medicine via SERS and Deep Learning.","authors":"Huixuan Yang, Mingyuan Chen, Jiayi Wang, Xufei Tong, Huiru Li, Hao Chen, Zengshan Yu, Chunying Zhao, Mingli Wang, Guochao Shi","doi":"10.3390/bios16030139","DOIUrl":"10.3390/bios16030139","url":null,"abstract":"In the modernization of traditional Chinese medicine (TCM), trace detection of pharmacodynamic substances faces a critical challenge: insufficient sensitivity, which significantly hinders accurate quality assessment and standardization. Conventional techniques often fail to measure trace components in complex sample matrices. Therefore, the development of a rapid, effective, sensitive, and reliable analytical method, along with a corresponding quality evaluation system, is of great importance. This study used moth wing (MW) scales as a template to fabricate an Ag/MW SERS substrate via magnetron sputtering. The optimal Ag30/MW SERS substrate (30 min sputtering) achieved an enhancement factor of 6.47 × 106 and good reproducibility (minimum RSD: 7.03%). Principal component analysis (PCA) was integrated with four deep learning algorithms (MLP, Transformer, ResNet, DNN) to detect three typical TCM pharmacodynamic substances in pure standard solutions: atractylon, cimifugin, and timosaponin A-III. The models enabled rapid identification, with the MLP model reaching 95.00% accuracy. This research provides a novel, highly accurate, and efficient detection method with potential for TCM pharmacodynamic substances, demonstrating feasibility for bioactive compound identification in model systems, and shows promising potential for future application in TCM composition analysis and quality control.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"16 3","pages":""},"PeriodicalIF":5.6,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13023520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147533760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Progress on Carbon-Dots-Based Probes for Microbial Labeling and Versatile Analysis Applications. 碳点探针在微生物标记和多功能分析中的应用进展。

IF 5.6 3区工程技术

Biosensors-Basel Pub Date : 2026-02-26 DOI: 10.3390/bios16030137

Ying Liu, Ping Yu, Jinhua Li, Yang Liu, Ming Ma, Sihua Qian, Yuhui Wang, Yunwei Wei

{"title":"Recent Progress on Carbon-Dots-Based Probes for Microbial Labeling and Versatile Analysis Applications.","authors":"Ying Liu, Ping Yu, Jinhua Li, Yang Liu, Ming Ma, Sihua Qian, Yuhui Wang, Yunwei Wei","doi":"10.3390/bios16030137","DOIUrl":"10.3390/bios16030137","url":null,"abstract":"Microbial imbalance and the spread of pathogenic microorganisms pose severe threats to human health and ecological security. Traditional microbial detection methods suffer from several drawbacks such as long detection time, low sensitivity, and insufficient specificity. As an emerging fluorescent probe, carbon dots (CDs) offer an innovative direction for microbial labeling and detection due to their ultra-small particle size, unique optical properties, excellent biocompatibility, and facile surface modifiability. Herein, this article reviews the research progress of CDs on microbial labeling and detection. The content covers a brief introduction of CDs and explores the main recognition strategies including non-covalent interactions and biomolecule-mediated targeted binding. It also elaborates on the application status of multi-modal sensing technologies for microbial detection, such as CDs-based fluorescent sensing, electrochemical sensing, and surface-enhanced Raman scattering (SERS) sensing. Additionally, the challenges faced in current research, such as achieving simultaneous detection of multiple pathogens and in vivo dynamic tracking, are analyzed, and the development prospects of CDs in fields like clinical diagnosis and public health monitoring are prospected. This review aims to provide comprehensive references for further research and application of CDs in the field of microbial detection.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"16 3","pages":""},"PeriodicalIF":5.6,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13023463/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147533755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface Plasmon Resonance as a Tool in Antiviral Drug Discovery Research. 表面等离子体共振作为抗病毒药物发现研究的工具。

IF 5.6 3区工程技术

Biosensors-Basel Pub Date : 2026-02-26 DOI: 10.3390/bios16030136

Katarzyna E Wegrzyn, John M Matsoukas

引用次数: 0

Proof of Concept for Tumor Mutational Burden Prediction Through Biophysical Analysis Based on UHF-Dielectrophoresis. 基于超高频介质电泳的生物物理分析预测肿瘤突变负荷的概念验证。

IF 5.6 3区工程技术

Biosensors-Basel Pub Date : 2026-02-25 DOI: 10.3390/bios16030134

Héloïse Daverat, Nina Blasco, Sandrine Robert, Amandine Rovini, Claire Dalmay, Fabrice Lalloué, Arnaud Pothier, Karine Durand, Thomas Naves

{"title":"Proof of Concept for Tumor Mutational Burden Prediction Through Biophysical Analysis Based on UHF-Dielectrophoresis.","authors":"Héloïse Daverat, Nina Blasco, Sandrine Robert, Amandine Rovini, Claire Dalmay, Fabrice Lalloué, Arnaud Pothier, Karine Durand, Thomas Naves","doi":"10.3390/bios16030134","DOIUrl":"10.3390/bios16030134","url":null,"abstract":"Tumor Mutational Burden (TMB) is a critical biomarker used to determine patient eligibility for immunotherapy with immune checkpoint inhibitors. However, its gold-standard assessment via whole exome sequencing is limited by high costs, technical complexity, and lengthy processing times. To address these challenges, we investigated whether Ultra-High-Frequency (UHF) electromagnetic wave sensing could serve as an alternative method for evaluating TMB. We analyzed the dielectrophoresis crossover frequency spectrum and corresponding electromagnetic signature (EMS) of cancer cells using a lab-on-a-chip biosensor that integrates microfluidics with dielectrophoresis-based electro-manipulation. Across seven solid tumor cell lines exhibiting diverse TMB levels, EMS exhibited an upward shift correlated with higher TMB, suggesting a relationship between mutational load and electromagnetic behavior. To further explore this connection, we artificially increased the somatic variant burden by exposing cells to the mutagen N-ethyl-N-nitrosourea (ENU). EMS measurements reliably detected the induced increase in variant load in ENU-treated cells. Overall, these findings demonstrate that EMS can detect both intrinsic TMB differences and experimentally induced increases in mutational burden, enabling refined categorization of cancer cells. Although further validation is required, this work lays the foundation for developing complementary, rapid, and accessible tools to support cancer cell stratification and guide immunotherapy decision-making.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"16 3","pages":""},"PeriodicalIF":5.6,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13023853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147533682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Immunochromatographic Test Strip and Its Application in Rapid Screening of Pepper Mild Mottle Virus. 免疫层析试纸条及其在辣椒轻度斑驳病毒快速筛选中的应用。

IF 5.6 3区工程技术

Biosensors-Basel Pub Date : 2026-02-25 DOI: 10.3390/bios16030135

Xin Yang, Kelei Han, Wenyao Zhang, Chen Zhang, Rui Fan, Tingtao Chen, Yan Jin, Jiashuo An, Zichen Zhu, Xiaolong Shao, Guoliang Qian, Dankan Yan, Limin Wang

{"title":"An Immunochromatographic Test Strip and Its Application in Rapid Screening of Pepper Mild Mottle Virus.","authors":"Xin Yang, Kelei Han, Wenyao Zhang, Chen Zhang, Rui Fan, Tingtao Chen, Yan Jin, Jiashuo An, Zichen Zhu, Xiaolong Shao, Guoliang Qian, Dankan Yan, Limin Wang","doi":"10.3390/bios16030135","DOIUrl":"10.3390/bios16030135","url":null,"abstract":"The quality and productivity of peppers have been severely impacted by the pepper mild mottle virus (PMMoV). To effectively control the transmission and damage caused by PMMoV, it is crucial to establish a simple, rapid, and field-applicable detection method. In this study, the coat protein of PMMoV was induced expression as an immunogen. After cell fusion and subcloning, a total of 10 hybridoma cell lines that stably secrete the PMMoV monoclonal antibody were screened. Through antibody pairing and screening, using monoclonal antibody 4D7 as the capture antibody and 3B9 as the detection antibody, an immunochromatographic test strip for PMMoV was established. Under ideal conditions, the test strip's color development indicated that its detection limit for the target protein was 1 ng/mL, and the result was obtained in 10 min. The findings of field testing and specific detection demonstrated that this test strip could reliably identify PMMoV and it is capable of distinguishing between different disease samples collected in this study. It is anticipated that this test strip will be able to offer services for PMMoV field detection.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"16 3","pages":""},"PeriodicalIF":5.6,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13024521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147533766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fungal Frontiers in (Bio)sensing. 真菌（生物）传感的前沿。

IF 5.6 3区工程技术

Biosensors-Basel Pub Date : 2026-02-22 DOI: 10.3390/bios16020131

Gerardo Grasso

{"title":"Fungal Frontiers in (Bio)sensing.","authors":"Gerardo Grasso","doi":"10.3390/bios16020131","DOIUrl":"10.3390/bios16020131","url":null,"abstract":"Filamentous fungi are increasingly recognized as versatile biological platforms for the development of advanced (bio)sensing technologies, owing to their extensive secretory capacity, material-forming ability, and intrinsic bioelectrical activity. This review critically surveys recent progress in fungal-based sensing within a multiscale framework spanning molecular, material, computational, and ecological domains, with particular emphasis on developments reported over the past five years. Key advances involving secretome-derived biomolecules, mycogenic nanomaterials, mycelium-based living materials, and fungal electrophysiology are discussed alongside emerging approaches for environmental monitoring that integrate sensor networks, imaging platforms, and data-driven analytics. Collectively, these works demonstrate that fungal systems can enhance biosensor sensitivity, selectivity, and sustainability, while enabling unconventional paradigms of signal transduction, material-integrated sensing, and biologically mediated computation. At larger spatial and temporal scales, mycelial growth dynamics and electrical activity provide measurable responses to mechanical, chemical, and environmental perturbations, supporting early applications in wearable devices, structural materials, and ecosystem monitoring. Despite significant progress, challenges remain in reproducibility, long-term stability, mechanistic understanding, and scalable device integration. Overall, the evidence reviewed highlights filamentous fungi as biologically adaptive and ecologically embedded systems with substantial potential to support next-generation (bio)sensing technologies, while underscoring the need for integrative approaches that combine biological insight with materials science, electronics, and artificial intelligence.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"16 2","pages":""},"PeriodicalIF":5.6,"publicationDate":"2026-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12938827/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147291410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Detection of Fumonisin B1 Using a Fluorescent Aptasensor with Plasmon-Modified Graphene Oxide as a Quencher. 用等离子体修饰氧化石墨烯作为猝灭剂的荧光体传感器快速检测伏马菌素B1。

IF 5.6 3区工程技术

Biosensors-Basel Pub Date : 2026-02-22 DOI: 10.3390/bios16020133

Yi Jiao, Xiaoqing Yang, Junping Hao, Yuhang Wen, Shanshan Wang, Jingbo Zhang, Hengchao E, Zhiyong Zhao, Jianhua Wang, Xianli Yang

{"title":"Rapid Detection of Fumonisin B1 Using a Fluorescent Aptasensor with Plasmon-Modified Graphene Oxide as a Quencher.","authors":"Yi Jiao, Xiaoqing Yang, Junping Hao, Yuhang Wen, Shanshan Wang, Jingbo Zhang, Hengchao E, Zhiyong Zhao, Jianhua Wang, Xianli Yang","doi":"10.3390/bios16020133","DOIUrl":"10.3390/bios16020133","url":null,"abstract":"Fumonisin B1 (FB1) is a secondary metabolite produced by Fusarium species, exhibiting strong toxicity and classified as a Group 2B carcinogen by the International Agency for Research on Cancer. It poses a significant threat to both human and animal health. Therefore, developing a simple and reliable method for FB1 detection and analysis is imperative. In this study, a biosensor based on nucleic acid aptamers was developed, utilizing plasma-modified graphene oxide (mGO) as a fluorescence quencher for FB1 detection. This system leverages the interaction between mGO and FAM-APT (a nucleic acid aptamer labeled with 5-carboxyfluorescein, FAM), achieving fluorescence quenching through fluorescence resonance energy transfer (FRET) under excitation at 490 nm and emission at 520 nm. In the presence of FB1, FAM-APT specifically binds to FB1 and dissociates from the mGO surface, resulting in fluorescence recovery. Quantitative detection of FB1 was achieved by measuring the differential fluorescence intensity. The biosensor demonstrated excellent linearity over a concentration range of 10 to 5 × 106 ng/L, with a detection limit (LOD) as low as 0.16 μg/L. Additionally, the sensor exhibited high specificity for FB1 among six common mycotoxins. In practical sample analysis, recovery rates ranged from 95.8% to 104.7% in corn samples and from 89.3% to 94.5% in rice samples. This aptamer-based biosensor features a simple structure, high sensitivity, and a wide detection range, providing important technical support for advancing mycotoxin research.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"16 2","pages":""},"PeriodicalIF":5.6,"publicationDate":"2026-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12937969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147291465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0