Biosensors-Basel最新文献_第10页

Advances in Machine Learning-Aided Thermal Imaging for Early Detection of Diabetic Foot Ulcers: A Review. 机器学习辅助热成像在糖尿病足溃疡早期检测中的研究进展

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2024-12-13 DOI: 10.3390/bios14120614

Longyan Wu, Ran Huang, Xiaoyan He, Lisheng Tang, Xin Ma

引用次数: 0

Establishment of Sample-to-Answer Loop-Mediated Isothermal Amplification-Based Nucleic Acid Testing Using the Sampling, Processing, Incubation, Detection and Lateral Flow Immunoassay Platforms. 利用采样、处理、孵育、检测和侧流免疫分析平台建立样本到应答环介导的等温扩增核酸检测。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2024-12-13 DOI: 10.3390/bios14120609

Lilas Pommiès, Hervé Boutal, David Fras, Hervé Volland

{"title":"Establishment of Sample-to-Answer Loop-Mediated Isothermal Amplification-Based Nucleic Acid Testing Using the Sampling, Processing, Incubation, Detection and Lateral Flow Immunoassay Platforms.","authors":"Lilas Pommiès, Hervé Boutal, David Fras, Hervé Volland","doi":"10.3390/bios14120609","DOIUrl":"10.3390/bios14120609","url":null,"abstract":"Diagnostics often require specialized equipment and trained personnel in laboratory settings, creating a growing need for point-of-care tests (POCTs). Among the genetic testing methods available, Loop-mediated Isothermal Amplification (LAMP) offers a viable solution for developing genetic POCT due to its compatibility with simplified devices. This study aimed to create a genetic test that integrates all steps from sample processing to analyzing results while minimizing the complexity, handling, equipment, and time required. Several challenges were addressed to achieve this goal: (1) the development of a buffer for bacterial DNA extraction that is compatible with both LAMP and immunochromatographic tests; (2) the adaption of the LAMP protocol for use with the SPID device; and (3) the optimization of the detection protocol for specific test conditions, with a lateral flow immunoassay format selected for its POCT compatibility. Following these developments, the test was validated using Escherichia coli (E. coli) and non-E. coli strains. A portable heating station was also developed to enable amplification without costly equipment. The resulting genetic POCT achieved 100% sensitivity and 85% specificity, with results available in 60 to 75 min. This study demonstrated that our POCT efficiently performs DNA extraction, amplification, and detection for bacterial identification. The test's simplicity and cost-effectiveness will support its implementation in various settings.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"14 12","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674888/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing Sensitivity of Point-of-Care Thyroid Diagnosis via Computational Analysis of Lateral Flow Assay Images Using Novel Textural Features and Hybrid-AI Models. 利用新型纹理特征和混合人工智能模型对侧流分析图像进行计算分析，提高即时甲状腺诊断的敏感性。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2024-12-13 DOI: 10.3390/bios14120611

Towfeeq Fairooz, Sara E McNamee, Dewar Finlay, Kok Yew Ng, James McLaughlin

{"title":"Enhancing Sensitivity of Point-of-Care Thyroid Diagnosis via Computational Analysis of Lateral Flow Assay Images Using Novel Textural Features and Hybrid-AI Models.","authors":"Towfeeq Fairooz, Sara E McNamee, Dewar Finlay, Kok Yew Ng, James McLaughlin","doi":"10.3390/bios14120611","DOIUrl":"10.3390/bios14120611","url":null,"abstract":"Lateral flow assays are widely used in point-of-care diagnostics but face challenges in sensitivity and accuracy when detecting low analyte concentrations, such as thyroid-stimulating hormone biomarkers. This study aims to enhance assay performance by leveraging textural features and hybrid artificial intelligence models. A modified Gray-Level Co-occurrence Matrix, termed the Averaged Horizontal Multiple Offsets Gray-Level Co-occurrence Matrix, was utilised to compute the textural features of the biosensor assay images. Significant textural features were selected for further analysis. A deep learning Convolutional Neural Network model was employed to extract features from these textural features. Both traditional machine learning models and hybrid artificial intelligence models, which combine Convolutional Neural Network features with traditional algorithms, were used to categorise these textural features based on the thyroid-stimulating hormone concentration levels. The proposed method achieved accuracy levels exceeding 95%. This pioneering study highlights the utility of textural aspects of assay images for accurate predictive disease modelling, offering promising advancements in diagnostics and management within biomedical research.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"14 12","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674693/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Imaging Applications of a Red-Emitting π-Acceptor (π-A) Pyrene-Benzothiazolium Dye. 发光π-受体（π-A）芘-苯并噻唑染料的成像应用研究。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2024-12-13 DOI: 10.3390/bios14120612

Chathura S Abeywickrama, Enya Huang, Wenhui Yan, Michael A Vrionides, Paaramitha Warushavithana, Kristen A Johnson, Robert V Stahelin, Yi Pang, Tomoyasu Mani, Kaveesha J Wijesinghe

{"title":"Exploring Imaging Applications of a Red-Emitting π-Acceptor (π-A) Pyrene-Benzothiazolium Dye.","authors":"Chathura S Abeywickrama, Enya Huang, Wenhui Yan, Michael A Vrionides, Paaramitha Warushavithana, Kristen A Johnson, Robert V Stahelin, Yi Pang, Tomoyasu Mani, Kaveesha J Wijesinghe","doi":"10.3390/bios14120612","DOIUrl":"10.3390/bios14120612","url":null,"abstract":"Bright biocompatible fluorescent imaging dyes with red to near-infrared (NIR) emissions are ideal candidates for fluorescence microscopy applications. Pyrene-benzothiazolium hemicyanine dyes are a new class of lysosome-specific probes reported on recently. In this work, we conduct a detailed implementation study for a pyrene-benzothiazolium derivative, BTP, to explore its potential imaging applications in fluorescence microscopy. The optical properties of BTP are studied in intracellular environments through advanced fluorescence microscopy techniques, with BTP exhibiting a noticeable shift toward blue (λem ≈ 590 nm) emissions in cellular lysosomes. The averaged photon arrival time (AAT)-based studies exhibit two different emissive populations of photons, indicating the probe's dynamic equilibrium between two distinctively different lysosomal microenvironments. Here, BTP is successfully utilized for time-lapse fluorescence microscopy imaging in real-time as a 'wash-free' imaging dye with no observed background interference. BTP exhibits an excellent ability to highlight microorganisms (i.e., bacteria) such as Bacillus megaterium through fluorescence microscopy. BTP is found to be a promising candidate for two-photon fluorescence microscopy imaging. The two-photon excitability of BTP in COS-7 cells is studied, with the probe exhibiting an excitation maximum at λTP ≈ 905 nm.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"14 12","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674487/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine Learning-Driven Innovations in Microfluidics. 微流体中机器学习驱动的创新。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2024-12-13 DOI: 10.3390/bios14120613

Jinseok Park, Yang Woo Kim, Hee-Jae Jeon

引用次数: 0

An MSRE-Assisted Glycerol-Enhanced RPA-CRISPR/Cas12a Method for Methylation Detection. msre辅助甘油增强RPA-CRISPR/Cas12a甲基化检测方法

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2024-12-12 DOI: 10.3390/bios14120608

Zhiquan Lu, Zilu Ye, Ping Li, Yike Jiang, Sanyang Han, Lan Ma

{"title":"An MSRE-Assisted Glycerol-Enhanced RPA-CRISPR/Cas12a Method for Methylation Detection.","authors":"Zhiquan Lu, Zilu Ye, Ping Li, Yike Jiang, Sanyang Han, Lan Ma","doi":"10.3390/bios14120608","DOIUrl":"10.3390/bios14120608","url":null,"abstract":"Background: Nasopharyngeal carcinoma (NPC) is a malignant tumor with high prevalence in southern China. Aberrant DNA methylation, as a hallmark of cancer, is extensively present in NPC, the detection of which facilitates early diagnosis and prognostic improvement of NPC. Conventional methylation detection methods relying on bisulfite conversion have limitations such as time-consuming, complex processes and sample degradation; thus, a more rapid and efficient method is needed.Methods: We propose a novel DNA methylation assay based on methylation-sensitive restriction endonuclease (MSRE) HhaI digestion and Glycerol-enhanced recombinase polymerase amplification (RPA)-CRISPR/Cas12a detection (HGRC). MSRE has a fast digestion rate, and HhaI specifically cleaves unmethylated DNA at a specific locus, leaving the methylated target intact to trigger the downstream RPA-Cas12a detection step, generating a fluorescence signal. Moreover, the detection step was supplemented with glycerol for the separation of Cas12a-containing components and RPA- and template-containing components, which avoids over-consumption of the template and, thus, enhances the amplification efficiency and detection sensitivity.Results: The HGRC method exhibits excellent performance in the detection of a CNE2-specific methylation locus with a (limit of detection) LOD of 100 aM and a linear range of 100 aM to 100 fM. It also responds well to different methylation levels and is capable of distinguishing methylation levels as low as 0.1%. Moreover, this method can distinguish NPC cells from normal cells by detecting methylation in cellular genomes. This method provides a rapid and sensitive approach for NPC detection and also holds good application prospects for other cancers and diseases featuring DNA methylation as a biomarker.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"14 12","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674872/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced Analytical Performance in CYFRA 21-1 Detection Using Lateral Flow Assay with Magnetic Bioconjugates: Integration and Comparison of Magnetic and Optical Registration. 使用磁性生物偶联物的横向流动试验增强CYFRA 21-1检测的分析性能：磁性和光学配准的整合和比较。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2024-12-11 DOI: 10.3390/bios14120607

Artemiy M Skirda, Alexey V Orlov, Juri A Malkerov, Sergey L Znoyko, Alexandra S Rakitina, Petr I Nikitin

{"title":"Enhanced Analytical Performance in CYFRA 21-1 Detection Using Lateral Flow Assay with Magnetic Bioconjugates: Integration and Comparison of Magnetic and Optical Registration.","authors":"Artemiy M Skirda, Alexey V Orlov, Juri A Malkerov, Sergey L Znoyko, Alexandra S Rakitina, Petr I Nikitin","doi":"10.3390/bios14120607","DOIUrl":"10.3390/bios14120607","url":null,"abstract":"A novel approach to developing lateral flow assays (LFAs) for the detection of CYFRA 21-1 (cytokeratin 19 fragment, a molecular biomarker for epithelial-origin cancers) is proposed. Magnetic bioconjugates (MBCs) were employed in combination with advanced optical and magnetic tools to optimize assay conditions. The approach integrates such techniques as label-free spectral-phase interferometry, colorimetric detection, and ultrasensitive magnetometry using the magnetic particle quantification (MPQ) technique. For the first time in LFA applications, the MPQ-based and colorimetry-based detection methods were compared side by side, and superior analytical performance was demonstrated. The limit of detection (LOD) of 0.9 pg/mL was achieved using MPQ, and 2.9 pg/mL with optical detection. This study has demonstrated that MPQ provides elimination of signal saturation, higher sensitivity (slope of the calibration curve), and a 19-fold wider dynamic range of detected signals. Both optical and magnetic detection results are comparable to the best laboratory-based tests with the added benefits of a 20-min assay duration and the LFA format convenience. The assay effectiveness was validated in human serum and artificial saliva, and high recovery rates were observed. The proposed approach offers rapid and reliable detection of molecular biomarkers and holds significant potential for point-of-care diagnostics, particularly in resource-limited settings.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"14 12","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial Responses to Bacterial Metabolic Activity and Biofilm Formation Studied Using Microbial Fuel Cell-Based Biosensors. 利用基于微生物燃料电池的生物传感器研究细菌代谢活性和生物膜形成的抗菌反应。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2024-12-11 DOI: 10.3390/bios14120606

Wenguo Wu, Huiya Hong, Jia Lin, Dayun Yang

{"title":"Antimicrobial Responses to Bacterial Metabolic Activity and Biofilm Formation Studied Using Microbial Fuel Cell-Based Biosensors.","authors":"Wenguo Wu, Huiya Hong, Jia Lin, Dayun Yang","doi":"10.3390/bios14120606","DOIUrl":"10.3390/bios14120606","url":null,"abstract":"Simultaneous monitoring of antimicrobial responses to bacterial metabolic activity and biofilm formation is critical for efficient screening of new anti-biofilm drugs. A microbial fuel cell-based biosensor using Pseudomonas aeruginosa as an electricigen was constructed. The effects of silver nanoparticles (AgNPs) on the cellular metabolic activity and biofilm formation of P. aeruginosa in the biosensors were investigated and compared with the traditional biofilm detection method. The crystal violet staining results showed that the concentration of AgNPs being increased to 20 and 40 μg/mL had a slight and obvious inhibitory effect on biofilm formation, respectively. In comparison, the detection sensitivity of the biosensor was much higher. When the concentration of AgNPs was 5 μg/mL, the output voltage of the biosensor was suppressed, and the inhibition gradually increased with the AgNPs dose. AgNPs inhibited the activity of planktonic cells in the anolyte and the formation of biofilm on the anode surface, and it had a dose-dependent effect on the secretion of phenazine in the anolyte. The biosensor could monitor the impacts of AgNPs not only on biofilm formation but also on cell activity and metabolic activity. It provides a new and sensitive method for the screening of anti-biofilm drugs.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"14 12","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674809/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical and Optical Multi-Detection of Escherichia coli Through Magneto-Optic Nanoparticles: A Pencil-on-Paper Biosensor. 利用磁光纳米粒子对大肠杆菌进行电化学和光学多重检测：一种笔对纸的生物传感器。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2024-12-10 DOI: 10.3390/bios14120603

Furkan Soysaldı, Derya Dincyurek Ekici, Mehmet Çağrı Soylu, Evren Mutlugun

{"title":"Electrochemical and Optical Multi-Detection of Escherichia coli Through Magneto-Optic Nanoparticles: A Pencil-on-Paper Biosensor.","authors":"Furkan Soysaldı, Derya Dincyurek Ekici, Mehmet Çağrı Soylu, Evren Mutlugun","doi":"10.3390/bios14120603","DOIUrl":"10.3390/bios14120603","url":null,"abstract":"Escherichia coli (E. coli) detection suffers from slow analysis time and high costs, along with the need for specificity. While state-of-the-art electrochemical biosensors are cost-efficient and easy to implement, their sensitivity and analysis time still require improvement. In this work, we present a paper-based electrochemical biosensor utilizing magnetic core-shell Fe2O3@CdSe/ZnS quantum dots (MQDs) to achieve fast detection, low cost, and high sensitivity. Using electrochemical impedance spectroscopy (EIS) as the detection technique, the biosensor achieved a limit of detection of 2.7 × 102 CFU/mL for E. coli bacteria across a concentration range of 102-108 CFU/mL, with a relative standard deviation (RSD) of 3.5781%. From an optical perspective, as E. coli concentration increased steadily from 104 to 107 CFU/mL, quantum dot fluorescence showed over 60% lifetime quenching. This hybrid biosensor thus provides rapid, highly sensitive E. coli detection with a fast analysis time of 30 min. This study, which combines the detection advantages of electrochemical and optical biosensor systems in a graphite-based paper sensor for the first time, has the potential to meet the needs of point-of-care applications. It is thought that future studies that will aim to examine the performance of the production-optimized, portable, graphite-based sensor system on real food samples, environmental samples, and especially medical clinical samples will be promising.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"14 12","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Distinct Second-Order Data Approaches for Thiamine Quantification via Carbon Dot/Silver Nanoparticle FRET Reversion. 通过碳点/银纳米粒子FRET还原探索硫胺素定量的不同二阶数据方法。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2024-12-10 DOI: 10.3390/bios14120604

Rafael C Castro, Ricardo N M J Páscoa, M Lúcia M F S Saraiva, João L M Santos, David S M Ribeiro

{"title":"Exploring Distinct Second-Order Data Approaches for Thiamine Quantification via Carbon Dot/Silver Nanoparticle FRET Reversion.","authors":"Rafael C Castro, Ricardo N M J Páscoa, M Lúcia M F S Saraiva, João L M Santos, David S M Ribeiro","doi":"10.3390/bios14120604","DOIUrl":"10.3390/bios14120604","url":null,"abstract":"Accurate and selective monitoring of thiamine levels in multivitamin supplements is essential for preventing deficiencies and ensuring product quality. To achieve this, a Förster resonance energy transfer (FRET) system using carbon dots (CDs) as energy donors and citrate-stabilized silver nanoparticles (AgNPs) as energy acceptors was developed. The aqueous synthesis of AgNPs using microwave irradiation was optimized to obtain efficient plasmonic nanoparticles for FRET applications, targeting maximal absorbance intensity, stability, and wavelength alignment. Using a central composite orthogonal design (CCOD), the optimal conditions were identified as a 12.5 min microwave reaction time, a Ag molar ratio of 0.72, and a pH of 8.28. The FRET sensing scheme was applied for thiamine determination, where the vitamin's presence impaired the FRET process, restoring CDs' photoluminescence (PL) emission in a concentration-dependent manner. To mitigate interference from other vitamins, PL kinetic data and excitation-emission matrix (EEM) data were analyzed using unfolded partial least-squares (U-PLS) with the subsequent application of the residual bilinearization technique (RBL), achieving high sensitivity and specificity for thiamine detection. This method demonstrated its accuracy and robustness by attaining a determination coefficient (R2) of 0.952 and a relative error of prediction (REP%) of 11%. This novel method offers highly sensitive and interference-free thiamine detection, with significant potential for a wide range of analytical applications.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"14 12","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0