Biosensors-Basel最新文献

{"title":"Melanin-Related Materials in Electrochemical Sensors for Monitoring the Environment and Food.","authors":"Agata Pane, Silvia Vicenzi, Chiara Mattioli, Dario Mordini, Arianna Menichetti, Marco Montalti","doi":"10.3390/bios15090631","DOIUrl":"10.3390/bios15090631","url":null,"abstract":"<p><p>Melanin-related materials efficiently emulate the adhesion properties of natural mussel filaments and have been used advantageously for surface modification and for fabrication of electrochemical sensors for detection of environmentally relevant targets. The most applicable advantages of melanin-based coatings are their biocompatibility and versatility, and they can be easily prepared and modified according to simple and highly environmentally friendly procedures. For these reasons, melanin-related materials, in particular polydopamine, which can be obtained simply via oxidative polymerization of dopamine in an aqueous solution in the presence of atmospheric oxygen, have been applied in a large variety of scientific and technological fields. Here, we summarize and critically discuss the most recent and important applications of melanin-related materials in the development of electrochemical sensors for monitoring the environment and food. In particular, the examples used in this paper include toxic metal ions, drugs, and pesticides. In the final section of this paper, the actual limitations of the existing approach are discussed and possible future design improvements are suggested.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SERS-Based Immunoassay for α-Fetoprotein Biomarker Detection Using an Au-Ag Nanostars Platform.","authors":"Josué Ismael García-Ramírez, Marcos Luna-Cervantes, Irma Yadira Izaguirre-Hernández, Julián Hernández-Torres, Enrique Juárez-Aguilar, Pablo Thomas-Dupont, José María Remes-Troche, Luis Zamora-Peredo","doi":"10.3390/bios15090632","DOIUrl":"10.3390/bios15090632","url":null,"abstract":"<p><p>Spiky Au-Ag nanostars offer intense plasmonic enhancement due to their sharp-tipped morphology, enabling powerful surface-enhanced Raman scattering (SERS). Here, we report a liquid-phase SERS platform that addresses current limitations in cancer biomarker detection, such as low sensitivity and dependence on Raman reporters. Nanostar concentration was tuned by simple centrifugation (10, 30, and 60 min), and their SERS performance was evaluated using methylene blue (MB) and mercaptopropionic acid (MPA) as probe molecules. Signal intensity scaled with nanostar content, enabling sensitive detection. Optimized nanostars were functionalized with MPA, 1-Ethyl-3-(3-dimethylamino1-Ethyl-3-(3dimethylaminopropyl1) carbodiimide (EDC), and N-Hydroxy succinimide (NHS) for covalent attachment of monoclonal anti-α-fetoprotein antibodies (AFP-Ab), facilitating the detection of AFP antigens across 167-38 ng/mL (antibody) and 500-0 ng/mL (antigen) ranges. The limit of detection (LOD) for the antigens was determined to be 16.73 ng/mL. Unlike conventional SERS systems, this aqueous, surfactant-free platform exploits the intrinsic vibrational modes of AFP, enabling sensitive and rapid biomarker detection with strong potential for early cancer diagnostics.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12468422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aptasensors for Rapid Detection of Hazards in Food: Latest Developments and Trends.","authors":"Anjie Guo, Yuan Zhang, Meifeng Jiang, Li Chen, Xinrong Jiang, Xiaobo Zou, Zongbao Sun","doi":"10.3390/bios15090629","DOIUrl":"10.3390/bios15090629","url":null,"abstract":"<p><p>The presence of hazardous substances in food poses a serious threat to our health. It is important to develop fast, convenient, and inexpensive assays for on-site sensitive analysis of various hazards in food. With the emergence and popularization of aptamers and biosensors, aptasensors have gradually become one of the most important detection techniques for substances such as nucleic acids and small molecules. This paper reviews the recent research progress in the field of aptasensor based on different technologies (such as electrochemistry, fluorescence, colorimetry, among others) for the rapid detection of hazards (such as foodborne pathogens, mycotoxins, pesticides, among others) in food. In addition, the current challenges of different aptasensors are described for the readers, and the future direction of aptasensors is envisioned by comparing the different technologies in order to develop a more suitable aptasensor. This review will not only promote the advancement of aptasensors but also their practical application in daily life to safeguard human health and food safety.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467590/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly Sensitive THz SPR Biosensor Based on Graphene-Coupled Prism Otto Structure.","authors":"Yu Xie, Zean Shen, Mingming Zhang, Mengjiao Ren, Wei Huang, Leyong Jiang","doi":"10.3390/bios15090630","DOIUrl":"10.3390/bios15090630","url":null,"abstract":"<p><p>This study presents a theoretical investigation of a terahertz (THz) surface plasmon resonance (SPR) optical biosensor utilizing a graphene-integrated Otto configuration. Through systematic numerical simulations, we demonstrate that actively modulating graphene's conductivity via an external magnetic field enables tunable SPR behavior with high phase sensitivity. The proposed sensor achieves a phase sensitivity of up to 3.1043×105 deg RIU^-1 in liquid sensing and 2.5854×104 deg RIU^-1 in gas sensing. This simulation-based work establishes a foundational framework for the development of highly sensitive, magneto-optically tunable optical sensors, highlighting their potential in chemical detection and medical diagnostics.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rolling Circle Amplification as a Molecular Tool for Spatially Resolved Signal Amplification in Single Molecule Counting Assay.","authors":"Juhwan Park","doi":"10.3390/bios15090628","DOIUrl":"10.3390/bios15090628","url":null,"abstract":"<p><p>There have been rising interests in ultra-sensitive biosensing technologies for early diagnosis and prognosis monitoring of infectious diseases, cancers, and neurodegenerative diseases. Digital signal readout strategy represented by digital ELISA or digital PCR, advanced biosensing field enormously, which enables detection of biomolecules under the detection limit of conventional biosensing methods. However, due to the need for compartmentalization and limited multiplex capability, it has been hurdled for utilization in applications requiring hierarchical resolution analysis such as sub-cellular molecules or molecular cargo of single cells or single extracellular vesicles (EVs). Rolling circle amplification (RCA), an isothermal DNA amplification method enabling localization of an amplified signal, can eliminate the need for compartmentalization and increase multiplex capability. It also has potential to expand applications of single molecule counting assay for understanding hierarchy of biological systems. In this review, recent advances in RCA-based single molecule counting assay are overviewed and their applications in single cells and single EVs quantitative analysis are discussed. Furthermore, the limitations and outlook of RCA-based single molecule counting assay are highlighted.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12468232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advances in Microfluidic Biofuel Cells.","authors":"Takahiro Kawaguchi, Shota Ito, Daisuke Nakane, Takashiro Akitsu","doi":"10.3390/bios15090627","DOIUrl":"10.3390/bios15090627","url":null,"abstract":"<p><p>Traditionally, fuel cells operate by using small fuel molecules such as hydrogen and methanol to produce energy, water, and carbon dioxide. Enzyme biofuel cells use enzymes rather than precious metals as electrode catalysts. In recent years, enzyme-immobilized electrodes have been developed by combining enzyme biofuel cells with microfluidic technology to improve the efficiency and performance of fuel cells. In this review, we will provide an overview and describe the current status of recent enzyme biofuel cells, microfluidic technology, and their applications to microfluidic fuel cells.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress and Prospects in FRET for the Investigation of Protein-Protein Interactions.","authors":"Yue Zhang, Xinyue Ma, Meihua Zhu, Vivien Ya-Fan Wang, Jiajia Guo","doi":"10.3390/bios15090624","DOIUrl":"10.3390/bios15090624","url":null,"abstract":"<p><p>Protein-protein interactions (PPIs) play a crucial role in various biological processes, including signal transduction, transcriptional regulation, and metabolic pathways. Over the years, many methods have been developed to study PPIs, such as yeast two-hybrid (Y2H), co-immunoprecipitation (Co-IP), pull-down assays, and surface plasmon resonance (SPR). However, each of these techniques has its own limitations, including false positives, a lack of specific binding partners, and restricted interaction zones. Fluorescence resonance energy transfer (FRET) has emerged as a powerful technique for investigating PPIs, offering several advantages over traditional methods. Recent advancements in fluorescence microscopy have further enhanced its application in PPI studies. In this review, we summarize recent developments in FRET-based approaches and their applications in PPIs research over the past five years, including conventional FRET, time-resolved FRET (TR-FRET), fluorescence lifetime imaging microscopy-FRET (FLIM-FRET), single-molecule FRET (smFRET), fluorescence cross-correlation spectroscopy FRET (FCCS-FRET), and provide guidance on selecting the most appropriate method for PPIs studies.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467375/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fiber Bragg Grating Embedded 3D-Printed Insole with Commercial and Portable Reader for Stance Phase Determination.","authors":"Arnaldo Leal-Junior, Mariana Silveira, Jan Nedoma, Radek Martinek","doi":"10.3390/bios15090623","DOIUrl":"10.3390/bios15090623","url":null,"abstract":"<p><p>This paper presents development and application of a Fiber Bragg Grating (FBG) array embedded in a 3D-printed insole for ground reaction force (GRF) estimation. In this case, a 3D-printed insole is fabricated from a scanned commercial insole in which a 5-FBGs array is integrated. The FBGs are characterized as a function of the applied transverse force, where a mean sensitivity of 0.11 ± 0.10 pm/N was obtained considering all FBGs. A portable FBG signal acquisition system was connected to the FBG array embedded in the insole and tested for the GRF analysis in a healthy volunteer. The gait tests results indicate stance and swing phases of 41.0 ± 6.5% and 59 ± 6.5%, respectively, which are within reference values of the literature. Furthermore, a 0.904 R² was found in the correlation analysis of the measured GRF response and the conventional M-shaped curve for the GRF in which all subdivisions of the stance phase were detected.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kinematic Biomarkers of Functional Disability in Older Adults: Analysis of the Timed Up and Go Test.","authors":"Juliana Moreira, Bruno Cunha, José Félix, Rubim Santos, Andreia S P Sousa","doi":"10.3390/bios15090621","DOIUrl":"10.3390/bios15090621","url":null,"abstract":"<p><p>The Timed Up and Go (TUG) test is used to assess mobility in older adults, but its reliance on completion time limits its insight into detailed movement patterns that could serve as early indicators of functional decline. This study aimed to identify lower limb and trunk kinematic biomarkers during the TUG test that distinguish between older adults with and without functional disability, emphasizing the potential for wearable sensor applications. Sixty adults aged 60+ participated in this cross-sectional study. Three-dimensional lower limb and trunk range of motion (ROM), velocity, center of mass (CoM) displacement, and velocity were analyzed using an optoelectronic system across TUG subphases: sit-to-walk, walk-forward, turn, walk-back, and turn-to-sit. Principal component analysis identified eleven principal components (PCs), explaining 84.33% of the total variance. PCs included sagittal hip and knee motion and CoM velocity during turn-to-sit and walking (PC1); tri-dimensional trunk velocity during turning, walk-back, and sit-to-walk transitions (PC2, PC4, PC6); sagittal knee and hip velocity in sit-to-walk (PC3); and frontal and transverse plane knee ROM and velocity during turning (PC5). Significant differences between functional disability groups were found for PC1 and PC4. These findings provide benchmark data for developing and validating wearable biosensors aimed at monitoring kinematic biomarkers.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467501/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced Electrochemical Sensors for Rapid and Sensitive Monitoring of Tryptophan and Tryptamine in Clinical Diagnostics.","authors":"Janani Sridev, Arif R Deen, Md Younus Ali, Wei-Ting Ting, M Jamal Deen, Matiar M R Howlader","doi":"10.3390/bios15090626","DOIUrl":"10.3390/bios15090626","url":null,"abstract":"<p><p>Tryptophan (Trp) and tryptamine (Tryp), critical biomarkers in mood regulation, immune function, and metabolic homeostasis, are increasingly recognized for their roles in both oral and systemic pathologies, including neurodegenerative disorders, cancers, and inflammatory conditions. Their rapid, sensitive detection in biofluids such as saliva-a non-invasive, real-time diagnostic medium-offers transformative potential for early disease identification and personalized health monitoring. This review synthesizes advancements in electrochemical sensor technologies tailored for Trp and Tryp quantification, emphasizing their clinical relevance in diagnosing conditions like oral squamous cell carcinoma (OSCC), Alzheimer's disease (AD), and breast cancer, where dysregulated Trp metabolism reflects immune dysfunction or tumor progression. Electrochemical platforms have overcome the limitations of conventional techniques (e.g., enzyme-linked immunosorbent assays (ELISA) and mass spectrometry) by integrating innovative nanomaterials and smart engineering strategies. Carbon-based architectures, such as graphene (Gr) and carbon nanotubes (CNTs) functionalized with metal nanoparticles (Ni and Co) or nitrogen dopants, amplify electron transfer kinetics and catalytic activity, achieving sub-nanomolar detection limits. Synergies between doping and advanced functionalization-via aptamers (Apt), molecularly imprinted polymers (MIPs), or metal-oxide hybrids-impart exceptional selectivity, enabling the precise discrimination of Trp and Tryp in complex matrices like saliva. Mechanistically, redox reactions at the indole ring are optimized through tailored electrode interfaces, which enhance reaction kinetics and stability over repeated cycles. Translational strides include 3D-printed microfluidics and wearable sensors for continuous intraoral health surveillance, demonstrating clinical utility in detecting elevated Trp levels in OSCC and breast cancer. These platforms align with point-of-care (POC) needs through rapid response times, minimal fouling, and compatibility with scalable fabrication. However, challenges persist in standardizing saliva collection, mitigating matrix interference, and validating biomarkers across diverse populations. Emerging solutions, such as AI-driven analytics and antifouling coatings, coupled with interdisciplinary efforts to refine device integration and manufacturing, are critical to bridging these gaps. By harmonizing material innovation with clinical insights, electrochemical sensors promise to revolutionize precision medicine, offering cost-effective, real-time diagnostics for both localized oral pathologies and systemic diseases. As the field advances, addressing stability and scalability barriers will unlock the full potential of these technologies, transforming them into indispensable tools for early intervention and tailored therapeutic monitoring in global healthcare.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467939/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}