Biosensors-Basel最新文献

The Personal Glucose Meter as the Measurement Principle in Point-of-Care Applications.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-19 DOI: 10.3390/bios15020121

Mònica Cano, Manel Del Valle

{"title":"The Personal Glucose Meter as the Measurement Principle in Point-of-Care Applications.","authors":"Mònica Cano, Manel Del Valle","doi":"10.3390/bios15020121","DOIUrl":"10.3390/bios15020121","url":null,"abstract":"A personal glucose meter (PGM) is a medical device that measures blood glucose levels and can be found worldwide. Owing to their sensitivity, simplicity, portability, and low cost, PGMs stand as one of the most frequently utilized analytical methods. This work reviews the different applied methodologies for detecting analytes other than glucose employing a PGM and how it can be incorporated for point-of-care diagnosis needs. To visualize the variants, first, a classification is made according to the biorecognition elements used (aptamers, antibodies, etc.), and where the determination of different analytes is done through the glucose signal using different glucose-generating enzymes such as invertase or glucosidase. Transduction can also be based on the use of nanocarriers that generally encapsulate glucose, although it is also possible to find a combination of the two aforementioned strategies. The PGM can also be used for the direct detection of interfering substances of the biosensor, such as NADH or paracetamol. Lastly, we discuss how a PGM might have been implemented to detect COVID-19 and how it could be used on a massive scale for the point-of-care diagnosis of a pandemic.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11852977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Precision Field- Effect Transistor Biosensor for Analyzing Differential Effects of Anti-Cancer Drugs on Cancerous and Non-Cancerous Cells.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-19 DOI: 10.3390/bios15020125

Can Li, Can Hu, Ling Li, Feng He, Xiaofei Li

{"title":"High-Precision Field- Effect Transistor Biosensor for Analyzing Differential Effects of Anti-Cancer Drugs on Cancerous and Non-Cancerous Cells.","authors":"Can Li, Can Hu, Ling Li, Feng He, Xiaofei Li","doi":"10.3390/bios15020125","DOIUrl":"10.3390/bios15020125","url":null,"abstract":"A high-precision biosensor technique is introduced, offering the capability to independently evaluate the effects of anti-cancer drugs on both cancerous (RAJI) and non-cancerous (WIL2S) cells. By analyzing and fitting current change curves and transfer characteristic curves under two drugs, camptothecin and doxorubicin, this technique quantifies both the magnitude of drug-induced current changes in cells and the rate of drug entry into cells. Flow cytometry was utilized to validate the entry rates of two drugs, camptothecin and doxorubicin, into the cells. The biosensor leverages the exceptional sensitivity of two-dimensional electron gas to detect proximal charge variations at ultralow concentrations, even in fluids with high ionic strength. The findings reveal that anti-cancer drugs have a more pronounced impact on tumor cells, with the effects and interaction speeds differing across normal cells and tumor cells. This innovative approach not only enhances our understanding of the specificity and action mechanisms of anti-cancer drugs but also provides a valuable tool for screening potential tumor anti-cancer drugs and advancing targeted cancer therapies.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11852787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogen Peroxide Fuel Cells and Self-Powered Electrochemical Sensors Based on the Principle of a Fuel Cell with Biomimetic and Nanozyme Catalysts.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-19 DOI: 10.3390/bios15020124

Yunong Zhang, Yuxin Liu, Andreas Offenhäusser, Yulia Mourzina

{"title":"Hydrogen Peroxide Fuel Cells and Self-Powered Electrochemical Sensors Based on the Principle of a Fuel Cell with Biomimetic and Nanozyme Catalysts.","authors":"Yunong Zhang, Yuxin Liu, Andreas Offenhäusser, Yulia Mourzina","doi":"10.3390/bios15020124","DOIUrl":"10.3390/bios15020124","url":null,"abstract":"The operating principle of a fuel cell is attracting increasing attention in the development of self-powered electrochemical sensors (SPESs). In this type of sensor, the chemical energy of the analyzed substance is converted into electrical energy in a galvanic cell through spontaneous electrochemical reactions, directly generating an analytical signal. Unlike conventional (amperometric, voltammetric, and impedimetric) sensors, no external energy in the form of an applied potential is required for the redox detection reactions to occur. SPESs therefore have several important advantages over conventional electrochemical sensors. They do not require a power supply and modulation system, which saves energy and costs. The devices also offer greater simplicity and are therefore more compatible for applications in wearable sensor devices as well as in vivo and in situ use. Due to the dual redox properties of hydrogen peroxide, it is possible to develop membraneless fuel cells and fuel-cell-based hydrogen peroxide SPESs, in which hydrogen peroxide in the analyzed sample is used as the only source of energy, as both an oxidant and a reductant (fuel). This also suppresses the dependence of the devices on the availability of oxygen. Electrode catalyst materials for different hydrogen peroxide reaction pathways at the cathode and the anode in a one-compartment cell are a key technology for the implementation and characteristics of hydrogen peroxide SPESs. This article provides an overview of the operating principle and designs of H2O2-H2O2 fuel cells and H2O2 fuel-cell-based SPESs, focusing on biomimetic and nanozyme catalysts, and highlights recent innovations and prospects of hydrogen-peroxide-based SPESs for (bio)electrochemical analysis.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11852683/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Latest Advances in Microfluidic DLD Cell Sorting Technology: The Optimization of Channel Design.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-19 DOI: 10.3390/bios15020126

Dan Fan, Yi Liu, Yaling Liu

引用次数: 0

Autofluorescence of Red Blood Cells Infected with P. falciparum as a Preliminary Analysis of Spectral Sweeps to Predict Infection.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-19 DOI: 10.3390/bios15020123

Miguel A Garrido-Tamayo, Alejandro Rincón Santamaría, Fredy E Hoyos, Tamara González Vega, David Laroze

{"title":"Autofluorescence of Red Blood Cells Infected with P. falciparum as a Preliminary Analysis of Spectral Sweeps to Predict Infection.","authors":"Miguel A Garrido-Tamayo, Alejandro Rincón Santamaría, Fredy E Hoyos, Tamara González Vega, David Laroze","doi":"10.3390/bios15020123","DOIUrl":"10.3390/bios15020123","url":null,"abstract":"Malaria, an infectious disease caused by parasites of the genus Plasmodium-including the most lethal species, Plasmodium falciparum-alters the physicochemical properties of host red blood cells, including their intrinsic autofluorescence after infecting them. This exploratory study aims to investigate the possibility of using autofluorescence as a method for detecting infection in red blood cells. The autofluorescence spectra of uninfected and in vitro infected red blood cells with Plasmodium falciparum were monitored and compared across an excitation wavelength range of 255 to 630 nm. Principal Component Analysis revealed that only two wavelengths (315 and 320 nm), previously undocumented, were able to accurately differentiate infected from uninfected red blood cells, showing an increase in autofluorescence in the ultraviolet and blue regions. This phenomenon is hypothetically associated with the presence of natural fluorophores such as tryptophan, FAD, NADH, porphyrins, and lipopigments. To classify the samples, Linear Discriminant Analysis (LDA) was employed, and Wilks' Lambda test confirmed that the discriminant function was significant, enabling correct classification of samples in more than 91% of cases. Overall, our results support the potential use of autofluorescence as an effective approach for detecting malaria parasite infection in red blood cells, with the possibility of implementation in portable devices for rapid field diagnostics.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11853592/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Wide-Range, Highly Stable Intelligent Flexible Pressure Sensor Based on Micro-Wrinkled SWCNT/rGO-PDMS with Efficient Thermal Shrinkage.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-19 DOI: 10.3390/bios15020122

Lei Fan, Zhaoxin Wang, Tao Yang, Qiang Zhao, Zhixin Wu, Yijie Wang, Xue Qi, Lei Zhang

{"title":"A Wide-Range, Highly Stable Intelligent Flexible Pressure Sensor Based on Micro-Wrinkled SWCNT/rGO-PDMS with Efficient Thermal Shrinkage.","authors":"Lei Fan, Zhaoxin Wang, Tao Yang, Qiang Zhao, Zhixin Wu, Yijie Wang, Xue Qi, Lei Zhang","doi":"10.3390/bios15020122","DOIUrl":"10.3390/bios15020122","url":null,"abstract":"Flexible pressure sensors have drawn growing attention in areas like human physiological signal monitoring and human-computer interaction. Nevertheless, it still remains a significant challenge to guarantee their long-term stability while attaining a wide detection range, a minute pressure testing limit, and high sensitivity. Inspired by the wrinkles on animal skins, this paper introduces a flexible pressure sensor with wrinkled microstructures. This sensor is composed of a composite of reduced graphene oxide (rGO), single-walled carbon nanotubes (SWCNTs), and polydimethylsiloxane (PDMS). After optimizing the proportion of the composite materials, the flexible pressure sensor was manufactured using highly efficient heat-shrinkable films. It has a sensitivity as high as 15.364 kPa-1. Owing to the wrinkled microstructures, the sensor can achieve an ultra-wide pressure detection range, with the maximum reaching 1150 kPa, and is capable of detecting water wave vibrations at the minimum level. Moreover, the wrinkled microstructures were locked by PDMS. The sensor acquired waterproof performance and its mechanical stability was enhanced. Even after 18,000 cycles of repeated loading and unloading, its performance remained unchanged. By combining with an artificial neural network, high-precision recognition of different sounds and postures when grasping different objects was realized, with the accuracies reaching 98.3333% and 99.1111%, respectively. Through the integration of flexible WIFI, real-time wireless transmission of sensing data was made possible. In general, the studied sensor can facilitate the application of flexible pressure sensors in fields such as drowning monitoring, remote traditional Chinese medicine, and intelligent voice.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11852995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Brief Review of Aptamer-Based Biosensors in Recent Years.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-18 DOI: 10.3390/bios15020120

Wenjing Wang, Yumin He, Suxiang He, Lei Deng, Hui Wang, Zhong Cao, Zemeng Feng, Benhai Xiong, Yulong Yin

引用次数: 0

Silicon-Based Biosensors: A Critical Review of Silicon's Role in Enhancing Biosensing Performance.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-18 DOI: 10.3390/bios15020119

Waqar Muhammad, Jaeyoon Song, Sehyeon Kim, Falguni Ahmed, Eunseo Cho, Huiseop Lee, Jinsik Kim

{"title":"Silicon-Based Biosensors: A Critical Review of Silicon's Role in Enhancing Biosensing Performance.","authors":"Waqar Muhammad, Jaeyoon Song, Sehyeon Kim, Falguni Ahmed, Eunseo Cho, Huiseop Lee, Jinsik Kim","doi":"10.3390/bios15020119","DOIUrl":"10.3390/bios15020119","url":null,"abstract":"This review into recent advancements in silicon-based technology, with a particular emphasis on the biomedical applications of silicon sensors. Owing to their diminutive size, high sensitivity, and intrinsic compatibility with electronic systems, silicon-based sensors have found widespread utilization across healthcare, industrial, and environmental monitoring domains. In the realm of biomedical sensing, silicon has demonstrated significant potential to enhance human health outcomes while simultaneously driving progress in microfabrication techniques for multifunctional device development. The review systematically examines the versatile roles of silicon in the fabrication of electrodes, sensing channels, and substrates. Silicon electrodes are widely used in electrochemical biosensors for glucose monitoring and neural activity recording, while sensing channels in field-effect transistor biosensors enable the detection of cancer biomarkers and small molecules. Porous silicon substrates are applied in optical biosensors for label-free protein and pathogen detection. Key challenges in this field, including the interaction of silicon with biomolecules, the economic barriers to miniaturization, and issues related to signal stability, are critically analyzed. Proposed strategies to address these challenges and improve sensor functionality and reliability are also discussed. Furthermore, the article explores emerging developments in silicon-based biosensors, particularly their integration into wearable technologies. The pivotal role of artificial intelligence (AI) in enhancing the performance, functionality, and real-time capabilities of these sensors is also highlighted. This review provides a comprehensive overview of the current state, challenges, and future directions in the field of silicon-based biomedical sensing technologies.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11852904/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual Monitoring of Blood Acetylcholinesterase Content and Catalytic Activity Utilizing Fluorometry-Integrated Surface Plasmon Resonance. 利用荧光测定法--集成表面等离子体共振对血液乙酰胆碱酯酶含量和催化活性进行双重监测

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-17 DOI: 10.3390/bios15020118

Yuanyuan Xie, Yifei Hou, Mengwei Hu, Hongzhuan Chen, Hao Wang, Lanxue Zhao, Jianrong Xu

{"title":"Dual Monitoring of Blood Acetylcholinesterase Content and Catalytic Activity Utilizing Fluorometry-Integrated Surface Plasmon Resonance.","authors":"Yuanyuan Xie, Yifei Hou, Mengwei Hu, Hongzhuan Chen, Hao Wang, Lanxue Zhao, Jianrong Xu","doi":"10.3390/bios15020118","DOIUrl":"10.3390/bios15020118","url":null,"abstract":"Acetylcholinesterase inhibitors (AChEIs), particularly donepezil, are commonly used to treat mild-to-moderate Alzheimer's disease (AD). However, drug accumulation during long-term use could change AChE activity and content, leading to peripheral side effects and prompting medication discontinuation. However, there are a lack of methods to simultaneously determine the content and catalytic activity of AChE. By using phosphatidylinositol-specific phospholipase C to strip AChE from erythrocyte surfaces, we developed a novel method combining surface plasmon resonance and fluorescence detection for the simultaneous detection of AChE content and activity, producing stable, reliable, and accurate results. The established determination range spans from 263.37 ng/mL to 3000 ng/mL (4.05 nM to 46.15 nM) for concentration, and from 39.02 mU/mL to 1000 mU/mL for activity. Compared to traditional methods, this approach simplifies operations, reduces detection time, expands the dynamic range, and lowers detection limits, potentially advancing AChE-related research and supporting clinical diagnostics and drug development.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11853072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transforming Sleep Monitoring: Review of Wearable and Remote Devices Advancing Home Polysomnography and Their Role in Predicting Neurological Disorders.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-17 DOI: 10.3390/bios15020117

Diana Vitazkova, Helena Kosnacova, Daniela Turonova, Erik Foltan, Martin Jagelka, Martin Berki, Michal Micjan, Ondrej Kokavec, Filip Gerhat, Erik Vavrinsky

{"title":"Transforming Sleep Monitoring: Review of Wearable and Remote Devices Advancing Home Polysomnography and Their Role in Predicting Neurological Disorders.","authors":"Diana Vitazkova, Helena Kosnacova, Daniela Turonova, Erik Foltan, Martin Jagelka, Martin Berki, Michal Micjan, Ondrej Kokavec, Filip Gerhat, Erik Vavrinsky","doi":"10.3390/bios15020117","DOIUrl":"10.3390/bios15020117","url":null,"abstract":"This paper explores the progressive era of sleep monitoring, focusing on wearable and remote devices contributing to advances in the concept of home polysomnography. We begin by exploring the basic physiology of sleep, establishing a theoretical basis for understanding sleep stages and associated changes in physiological variables. The review then moves on to an analysis of specific cutting-edge devices and technologies, with an emphasis on their practical applications, user comfort, and accuracy. Attention is also given to the ability of these devices to predict neurological disorders, particularly Alzheimer's and Parkinson's disease. The paper highlights the integration of hardware innovations, targeted sleep parameters, and partially advanced algorithms, illustrating how these elements converge to provide reliable sleep health information. By bridging the gap between clinical diagnosis and real-world applicability, this review aims to elucidate the role of modern sleep monitoring tools in improving personalised healthcare and proactive disease management.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11853583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0