A Mobile Sperm Analyzer with User-Friendly Microfluidic Chips for Rapid On-Farm Semen Evaluation.

IF 4.9 3区工程技术 Q1 CHEMISTRY, ANALYTICAL

Biosensors-Basel Pub Date : 2025-06-18 DOI:10.3390/bios15060394

Shu-Sheng Lin, Chang-Yu Chen, Cheng-Ming Lin, Tsun-Chao Chiang, Yu-Siang Tang, Chang-Ching Yeh, Wei-Fan Hsu, Andrew M Wo

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Abstract

This study presents a mobile-based sperm analysis system featuring a user-friendly, droplet-loaded microfluidic chip that enables non-specialist users to perform the rapid and accurate quantitative evaluation of boar semen directly on the farm. The iSperm system integrates a tablet, optical module, heater, and real-time image analysis app to deliver automated measurements of sperm concentration, motility, and progressive motility in under one minute. Precision and user variability tests demonstrated high concordance with CASA and the hemocytometer, with minimal differences between trained and untrained users. A method comparison using 77 farm-collected samples confirmed agreement through Passing-Bablok regression and Bland-Altman analysis. ROC curve analyses further validated diagnostic accuracy for all parameters, with AUC values exceeding 0.95. The iSperm platform offers a reliable, user-friendly, and field-deployable solution for on-site semen quality assessment, improving decision-making in swine artificial insemination.

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一种带有用户友好微流控芯片的移动精子分析仪，用于农场精液的快速评估。

本研究提出了一种基于移动的精子分析系统，该系统具有用户友好的微流控芯片，使非专业用户能够直接在农场对野猪精液进行快速准确的定量评估。iSperm系统集成了平板电脑、光学模块、加热器和实时图像分析应用程序，可在一分钟内自动测量精子浓度、活力和渐进活力。精确度和用户可变性测试表明，CASA和血细胞计具有高度一致性，训练有素和未训练的用户之间的差异极小。通过Passing-Bablok回归和Bland-Altman分析，对77个农场采集的样本进行了方法比较。ROC曲线分析进一步验证了各参数的诊断准确性，AUC值均超过0.95。iSperm平台为现场精液质量评估提供了一个可靠的、用户友好的、可现场部署的解决方案，提高了猪人工授精的决策。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry

CiteScore

6.60

自引率

14.80%

发文量

983

审稿时长

11 weeks

期刊介绍： Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.