Biosensors-Basel最新文献_第8页

Molecularly Imprinted SERS Plasmonic Sensor for the Detection of Malachite Green. 分子印迹SERS等离子体传感器检测孔雀石绿。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-20 DOI: 10.3390/bios15050329

Hao Zhang, Dani Sun, Yuhao Wen, Mengyuan Wang, Jingying Huang, Ziru Lian, Jinhua Li

{"title":"Molecularly Imprinted SERS Plasmonic Sensor for the Detection of Malachite Green.","authors":"Hao Zhang, Dani Sun, Yuhao Wen, Mengyuan Wang, Jingying Huang, Ziru Lian, Jinhua Li","doi":"10.3390/bios15050329","DOIUrl":"10.3390/bios15050329","url":null,"abstract":"Malachite green (MG) is a highly toxic dye commonly used in industries and aquaculture, leading to significant environmental contamination and health hazards. Therefore, sensitive and selective detection of MG in real samples is urgently needed. This study presents the development of a molecularly imprinted surface-enhanced Raman spectroscopy (MI-SERS) plasmonic sensor for the rapid and sensitive detection of MG. The sensor consists of a gold nanostar (Au NS) layer as the SERS substrate and an imprinted polydopamine layer containing specific recognition sites for MG. Taking full advantage of the plasmonic effect of SERS and selective recognition capability of imprinted materials, under optimized conditions, the sensor demonstrated high sensitivity, with a detection limit of 3.5 × 10-3 mg/L, excellent selectivity against interference from other organic dyes, and robust performance with recoveries of 90.2-114.2% in real seawater samples. The MI-SERS sensor also exhibited good reproducibility, stability, and reusability. These findings suggest that the MI-SERS sensor is a promising tool for real-time monitoring of MG contamination in complicated samples.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12110142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Advances in Paper-Based Electronics: Emphasis on Field-Effect Transistors and Sensors. 纸基电子学的最新进展：着重于场效应晶体管和传感器。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-19 DOI: 10.3390/bios15050324

Dimitris Barmpakos, Apostolos Apostolakis, Fadi Jaber, Konstantinos Aidinis, Grigoris Kaltsas

{"title":"Recent Advances in Paper-Based Electronics: Emphasis on Field-Effect Transistors and Sensors.","authors":"Dimitris Barmpakos, Apostolos Apostolakis, Fadi Jaber, Konstantinos Aidinis, Grigoris Kaltsas","doi":"10.3390/bios15050324","DOIUrl":"10.3390/bios15050324","url":null,"abstract":"Paper-based electronics have emerged as a sustainable, low-cost, and flexible alternative to traditional substrates for electronics, particularly for disposable and wearable applications. This review outlines recent developments in paper-based devices, focusing on sensors and paper-based field-effect transistors (PFETs). Key fabrication techniques such as laser-induced graphene, inkjet printing, and screen printing have enabled the creation of highly sensitive and selective devices on various paper substrates. Material innovations, especially the integration of graphene, carbon-based materials, conductive polymers, and other novel micro- and nano-enabled materials, have significantly enhanced device performance. This review discusses modern applications of paper-based electronics, with a particular emphasis on biosensors, electrochemical and physical sensors, and PFETs designed for flexibility, low power, and high sensitivity. Advances in PFET architectures have further enabled the development of logic gates and memory systems on paper, highlighting the potential for fully integrated circuits. Despite challenges in durability and performance consistency, the field is rapidly evolving, driven by the demand for green electronics and the need for decentralized, point-of-care diagnostic tools. This paper also identifies detection strategies used in paper-based sensors, reviews limitations in the current fabrication methods, and outlines opportunities for the scalable production of multifunctional paper-based systems. This review addresses a critical gap in the literature by linking device-level innovation with real-world sensor applications on paper substrates.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Nanoparticle-Based Immunoassay on Facemasks for Evaluating Neutrophilic Airway Inflammation in COPD Patients. 基于纳米颗粒的面罩免疫分析法评估慢性阻塞性肺病患者中性粒细胞气道炎症。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-19 DOI: 10.3390/bios15050323

Bartomeu Mestre, Nuria Toledo-Pons, Andreu Vaquer, Sofia Tejada, Antonio Clemente, Amanda Iglesias, Meritxell López, Ruth Engonga, Sabina Perelló, Borja G Cosío, Roberto de la Rica

{"title":"A Nanoparticle-Based Immunoassay on Facemasks for Evaluating Neutrophilic Airway Inflammation in COPD Patients.","authors":"Bartomeu Mestre, Nuria Toledo-Pons, Andreu Vaquer, Sofia Tejada, Antonio Clemente, Amanda Iglesias, Meritxell López, Ruth Engonga, Sabina Perelló, Borja G Cosío, Roberto de la Rica","doi":"10.3390/bios15050323","DOIUrl":"10.3390/bios15050323","url":null,"abstract":"Patients with chronic obstructive pulmonary disease (COPD) often experience acute exacerbations characterized by elevated neutrophilic inflammation in the lungs. Currently, this condition is diagnosed through visual inspection of sputum color and volume, a method prone to personal bias and unsuitable for patients who are unable to expectorate spontaneously. In this manuscript, we present a novel approach for measuring and monitoring exhaled myeloperoxidase (MPO), a biomarker of neutrophilic airway inflammation, without the need for sputum analysis. The method involves analyzing an unmodified surgical facemask worn by the patient for 30 min using biosensing decals that transfer antibody-coated nanoparticles. These colloids specifically interact with MPO trapped by the facemask in a dose-dependent manner, enabling the quantification of MPO levels, with a dynamic range up to 3 · 101 µg·mL-1. The proposed diagnostic approach successfully differentiated patients with acute exacerbations from stable patients with 100% sensitivity and specificity. Healthy individuals also showed significantly lower MPO levels compared to COPD patients. Our results suggest that facemask analysis could be a non-invasive diagnostic tool for airway diseases, particularly in patients unable to expectorate.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12110278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasensitive and Real-Time Detection of Kanamycin Residues in Milk Using an Aptasensor Based on Microfluidic Capacitive Strategy. 基于微流控电容策略的适体传感器超灵敏实时检测牛奶中卡那霉素残留。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-18 DOI: 10.3390/bios15050322

Weidong Zheng, Jun Chai, Jayne Wu, Jian Zhang, Haochen Qi

{"title":"Ultrasensitive and Real-Time Detection of Kanamycin Residues in Milk Using an Aptasensor Based on Microfluidic Capacitive Strategy.","authors":"Weidong Zheng, Jun Chai, Jayne Wu, Jian Zhang, Haochen Qi","doi":"10.3390/bios15050322","DOIUrl":"10.3390/bios15050322","url":null,"abstract":"Kanamycin (KanR) is a widely used antibiotic in human and veterinary medicine, as well as in food production and livestock breeding. However, its environmental residue and bioaccumulation in the food chain pose a great threat to human health. A real-time and sensitive aptasensor is developed for KanR detection based on a gold interdigitated electrode (IDE). A microfluidic alternating current electrothermal (ACET) effect is employed for rapid directional manipulation and enrichment of KanR molecules. As an ultrasensitive indicator, solid-liquid capacitance is adopted to reflect the tiny change on the IDE surface caused by target adsorption. The overall detection takes only 60 s from sample to result, and a wide linear detection range of 0.1 fM~1 pM, an ultra-low detection limit of 16.56 aM, and a high selectivity of 7752:1 are simultaneously achieved, with 5 times of repeated use and the shelf life of 10 days. Furthermore, the aptasensor shows excellent practicability in milk samples, with the spiked recovery rate ranging from 86.90% to 116.17%. This aptasensor with the detecting strategy provides a rapid, convenient, and cost-effective solution for real-time monitoring of KanR.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12110345/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MIP-Modified Porous Silicon Optical Sensor for Interleukin-6 Label-Free Quantification. 用于白细胞介素-6无标记定量的mip修饰多孔硅光学传感器

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-17 DOI: 10.3390/bios15050320

Valeria Nocerino, Giulia Siciliano, Monica Bianco, Ilaria Rea, Principia Dardano, Maria Serena Chiriacò, Francesco Ferrara, Giuseppe Gigli, Elisabetta Primiceri, Luca De Stefano

{"title":"MIP-Modified Porous Silicon Optical Sensor for Interleukin-6 Label-Free Quantification.","authors":"Valeria Nocerino, Giulia Siciliano, Monica Bianco, Ilaria Rea, Principia Dardano, Maria Serena Chiriacò, Francesco Ferrara, Giuseppe Gigli, Elisabetta Primiceri, Luca De Stefano","doi":"10.3390/bios15050320","DOIUrl":"10.3390/bios15050320","url":null,"abstract":"In this study, we present an innovative optical biosensor designed to detect Interleukin-6 (IL-6), a pivotal cytokine implicated in many pathological conditions. Our sensing platform is made of a porous silicon (PSi) nanostructured substrate modified with a thin (~5 nm) molecularly imprinted polymer (MIP), ensuring both high specificity and sensitivity toward IL-6 molecules. The fabrication process involves electrochemical etching of silicon chips to create the porous structure, followed by the electrodeposition of the MIP, which is tailored to selectively bind the IL-6 target. Extensive testing over a broad IL-6 concentration range demonstrates a clear, proportional optical response, yielding a limit of detection (LOD) of 13 nM. Moreover, the biosensor robustness was verified by evaluating its performance in bovine serum, a complex biological matrix. Despite the presence of various interfering components, the sensor maintained its selectivity and displayed minimal matrix effects, underlining its practical applicability in real-world diagnostic scenarios.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109668/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Highly Sensitive Electrochemical Immunosensor for Cortisol Detection. 一种用于皮质醇检测的高灵敏度电化学免疫传感器。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-17 DOI: 10.3390/bios15050321

Pritu Parna Sarkar, Ali Ashraf, Ahmed Hasnain Jalal, Fahmida Alam, Nazmul Islam

{"title":"A Highly Sensitive Electrochemical Immunosensor for Cortisol Detection.","authors":"Pritu Parna Sarkar, Ali Ashraf, Ahmed Hasnain Jalal, Fahmida Alam, Nazmul Islam","doi":"10.3390/bios15050321","DOIUrl":"10.3390/bios15050321","url":null,"abstract":"In this research, an interdigitated gear-shaped working electrode is presented for cortisol sensing. Overall, the sensor was designed in a three-electrode system and was fabricated using direct laser scribing. A synthesized conductive ink based on graphene and polyaniline was further employed to enhance the electrochemical performance of the sensor. Scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy were employed for physicochemical characterization of the laser-induced graphene (LIG) sensor. Cortisol, a biomarker essential in detecting stress, was detected both in phosphate-buffered saline (PBS, pH = 7.4) and human serum within a linear range of 100 ng/mL to 100 µg/mL. Ferri/ferrocyanide was employed as the redox probe to detect cortisol in PBS. The electrochemical performance of the developed sensor was assessed via differential pulse voltammetry (DPV), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and chronoamperometry. The electrochemical performance demonstrates high sensitivity and selectivity alongside strong repeatability (relative standard deviation (RSD) = 3.8%, n = 4) and reproducibility (RSD = 5.85%, n = 5). Overall, these results highlight the sensor's reliability, high sensitivity, and repeatability and reproducibility in the detection of cortisol. The sensor successfully detected cortisol in the complex medium of human serum and effectively distinguished it in a ternary mixture containing cortisol and dopamine. Also, the use of direct laser writing on Kapton film makes the approach cost-effective and thus disposable, making it suitable for chronic stress diagnostics and neurological research applications.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12110703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colorimetric Detection of microRNA-378 Based on Y-Shaped Structure Formed by Gold Nanoparticles and Catalytic Hairpin Self-Assembly. 基于金纳米颗粒形成的y形结构和催化发夹自组装的microRNA-378比色检测。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-15 DOI: 10.3390/bios15050319

Yahui Gao, Jinru Pan, Bingyuan Fan, Shan Wang, Qian Wang, Wanru Liu, Fang Hu, Wei Meng

{"title":"Colorimetric Detection of microRNA-378 Based on Y-Shaped Structure Formed by Gold Nanoparticles and Catalytic Hairpin Self-Assembly.","authors":"Yahui Gao, Jinru Pan, Bingyuan Fan, Shan Wang, Qian Wang, Wanru Liu, Fang Hu, Wei Meng","doi":"10.3390/bios15050319","DOIUrl":"10.3390/bios15050319","url":null,"abstract":"The timely and accurate detection of cancer is crucial for preventing disease progression and for the early treatment of confirmed cases. MiRNAs are cancer markers. In this study, a simple miRNA detection method is proposed. Three hairpins were designed based on gold nanoparticles combined with catalytic hairpin assembly nucleic acid amplification technology. The low-pH method was used for rapid coupling, and hairpin H1 was opened by miR-378, triggering the cycle reaction and signal amplification and finally forming a Y-shaped structure, thereby narrowing the distance between gold nanoparticles and achieving colorimetric detection. The absorbance change (A620/A520) was proportional to the concentration of miR-378 (0.05-5 nM), with a detection limit of 0.05 nM. This method also has an evident detection effect on real samples. HeLa and L-02 cell extracts were analyzed using this method. The former showed no obvious color change, whereas the maximum absorption peak of the latter showed a red shift, and the color changed from red to purple. The minimum number of cells that could be detected using HeLa cells was 500 cells/mL.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12110092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasensitive Bead-Based Immunoassay for Real-Time Continuous Sample Flow Analysis. 用于实时连续样品流分析的超灵敏珠免疫分析法。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-15 DOI: 10.3390/bios15050316

Yuri M Shlyapnikov, Elena A Shlyapnikova

{"title":"Ultrasensitive Bead-Based Immunoassay for Real-Time Continuous Sample Flow Analysis.","authors":"Yuri M Shlyapnikov, Elena A Shlyapnikova","doi":"10.3390/bios15050316","DOIUrl":"10.3390/bios15050316","url":null,"abstract":"The performance of heterophase immunoassays is often limited by the kinetics of analyte binding. This problem is partially solved by bead-based assays, which are characterized by rapid diffusion in the particle suspension. However, at low analyte concentrations, the binding rate is still low. Here, we demonstrate a further improvement of analyte binding kinetics in bead-based immunoassays by simultaneously concentrating both an analyte and magnetic beads in a compact spatial region where binding occurs. The analyte is electrophoretically concentrated in a flow cell where beads are magnetically retained and dragged along the channel by viscous force. The flow cell is integrated with a microarray-based signal detection module, where beads with bound analyte scan the microarray surface and are retained on it by single specific interactions, assuring ultra-high sensitivity of the method. Thus, a continuous flow assay system is formed. Its performance is demonstrated by simultaneous detection of model pathogen biomarkers, cholera toxin (CT) and staphylococcal enterotoxin B (SEB), with a detection limit of 0.1 fM and response time of under 10 min. The assay is capable of real-time online sample monitoring, as shown by a 12 h long continuous flow analysis of tap water for SEB and CT.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Immunoassays for Foodborne Pathogenic Bacteria Detection Using PolyHRP for Signal Enhancement. 多hrp信号增强法检测食源性致病菌的研究进展。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-15 DOI: 10.3390/bios15050318

Yijia Zhang, Junkang Pan, Qiyi He, Zhihao Xu, Bruce D Hammock, Dongyang Li

{"title":"Development of Immunoassays for Foodborne Pathogenic Bacteria Detection Using PolyHRP for Signal Enhancement.","authors":"Yijia Zhang, Junkang Pan, Qiyi He, Zhihao Xu, Bruce D Hammock, Dongyang Li","doi":"10.3390/bios15050318","DOIUrl":"10.3390/bios15050318","url":null,"abstract":"The rapid and accurate detection of foodborne pathogens is essential for ensuring food safety. Escherichia coli O157:H7 (E. coli O157:H7) and Salmonella Typhimurium (S. Typhimurium) are major foodborne pathogenic bacteria that pose significant public health risks, highlighting the need for effective detection methods. In this study, highly sensitive double-antibody sandwich-based enzyme-linked immunosorbent assays (ELISAs) were developed for the rapid detection of E. coli O157:H7 and S. Typhimurium, utilizing a streptavidin-polymerized horseradish peroxidase (SA-PolyHRP)-based signal enhancement system. Systematic optimization was performed on key parameters, including the capture antibody concentration, detection antibody, and blocking agent. Compared to the method using SA-HRP, substitution with SA-PolyHRP significantly improved detection sensitivity, achieving limits of detection (LODs) of 1.4 × 104 CFU/mL for E. coli O157:H7 and 6.0 × 103 CFU/mL for S. Typhimurium, with sensitivity enhancements of 7.86-fold and 1.83-fold, respectively. Specificity tests confirmed no cross-reactivity with non-target or closely related pathogenic strains. The matrix effect was effectively mitigated through 10-fold and 100-fold dilutions for E. coli O157:H7 and S. Typhimurium, respectively. Both pathogens were successfully detected in beef samples spiked with 5 CFU after 5 h of incubation. This study demonstrates the effectiveness of PolyHRP-based signal enhancement for the highly sensitive and specific detection of foodborne pathogens, offering a promising approach for rapid food safety monitoring and public health protection.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109803/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-Pot Detection of miRNA by Dual Rolling Circle Amplification at Ambient Temperature with High Specificity and Sensitivity. 室温双滚圈扩增一锅检测miRNA的高特异性和灵敏度。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-15 DOI: 10.3390/bios15050317

Wenhua Sun, Kunling Hu, Ziting Song, Ran An, Xingguo Liang

{"title":"One-Pot Detection of miRNA by Dual Rolling Circle Amplification at Ambient Temperature with High Specificity and Sensitivity.","authors":"Wenhua Sun, Kunling Hu, Ziting Song, Ran An, Xingguo Liang","doi":"10.3390/bios15050317","DOIUrl":"10.3390/bios15050317","url":null,"abstract":"Rolling circle amplification (RCA) at ambient temperature is prone to false positive signals during nucleic acid detection, which makes it challenging to establish an efficient RCA detection method. The false positive signals are primarily caused by binding of non-target nucleic acids to the circular single-stranded template, leading to non-specific amplification. Here, we present an RCA method for miRNA detection at 37 °C using two circular ssDNAs, each of which is formed by ligating the intramolecularly formed nick (without any splint) in a secondary structure. The specific target recognition is realized by utilizing low concentrations (0.1 nM) of circular ssDNA1 (C1). A phosphorothioate modification is present at G*AATTC on C1 to generate a nick for primer extension during the primer self-generated rolling circle amplification (PG-RCA). The fragmented amplification products are used as primers for the following RCA that serves as signal amplification using circular ssDNA2 (C2). Notably, the absence of splints and the low concentration of C1 significantly inhibits non-target binding, thus minimizing false positive signals. A high concentration (10 nM) of C2 is used to carry out linear rolling circle amplification (LRCA), which is highly specific. This strategy demonstrates a good linear response to 0.01-100 pM of miRNA with a detection limit of 7.76 fM (miR-155). Moreover, it can distinguish single-nucleotide mismatch in the target miRNA, enabling the rapid one-pot detection of miRNA at 37 °C. Accordingly, this method performs with high specificity and sensitivity. This approach is suitable for clinical serum sample analysis and offers a strategy for developing specific biosensors and diagnostic tools.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109915/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0