Biosensors-Basel最新文献

{"title":"Development of a QCM-D-Based Aptasensor for the Real-Time Detection of β-Lactoglobulin.","authors":"Tuba Çanak-Ipek, Melis Güler Girbas, Nicolas Casadei, Christian Schlensak, Anna-Kristina Marel, Meltem Avci-Adali","doi":"10.3390/bios15090563","DOIUrl":"10.3390/bios15090563","url":null,"abstract":"<p><p>The prevalence of food allergies has been steadily increasing in recent years. β-lactoglobulin (β-LG), the main allergenic protein of milk and dairy allergies, is more commonly observed in infants and children. In this study, a β-LG-specific aptamer was selected using the combinatorial chemistry process known as systematic evolution of ligands by exponential enrichment (SELEX), and a quartz crystal microbalance with dissipation monitoring (QCM-D)-based aptasensor was developed using a novel surface functionalization technique, which mimics an artificial cell membrane on the QCM-D sensor surface, creating a physiologically relevant environment for the binding of the target to the sensor. Through SELEX combined with next-generation sequencing (NGS), the aptamer Apt 356 was identified. Its binding to β-LG was confirmed via dot blot analysis. The selected Apt 356 was then used for the development of a QCM-D-based sensor. To fabricate the sensor, the quartz surface was functionalized with a supported lipid bilayer (SLB). The β-LG-specific aptamer was immobilized onto this SLB. The results demonstrated that the QCM-D system allows real-time observation and evaluation of the binding of β-LG. While there have been some studies on aptasensors for the β-LG protein, to the best of our knowledge, this is the first QCM-D-based aptasensor developed specifically for β-LG protein detection.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12468009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Design and Application of Wearable Ultrasound Devices for Detection and Imaging.","authors":"Yuning Lei, Jinjie Duan, Qi Qi, Jie Fang, Qian Liu, Shuang Zhou, Yuxiang Wu","doi":"10.3390/bios15090561","DOIUrl":"10.3390/bios15090561","url":null,"abstract":"<p><p>The convergence of flexible electronics and miniaturized ultrasound transducers has accelerated the development of wearable ultrasound devices, offering innovative solutions for continuous, non-invasive physiological monitoring and disease diagnosis. This review systematically examines the recent progress in the field, focusing on three key aspects: physical principles, device design, and clinical applications. From the perspective of physical principles, we provide an in-depth analysis of the fundamental theories underlying ultrasound imaging, including acoustic wave propagation in biological tissues, interface reflection mechanisms, and Doppler effects. In terms of device design, we compare technical approaches for rigid and flexible ultrasound transducers, with particular emphasis on innovative designs for flexible transducers. The key developments discussed include optimization of piezoelectric materials, the fabrication of stretchable electrodes, and advances in flexible encapsulation materials. Regarding clinical applications, we categorize the use cases by anatomical region and illustrate their diagnostic value through representative examples, demonstrating their utility in disease detection, health monitoring, and sports medicine. Finally, we identify critical challenges such as signal stability, coupling material compatibility, and long-term wearability, while outlining future directions including AI-assisted diagnosis and multifunctional integration. This review aims to provide a comprehensive reference for both fundamental research and clinical translation of wearable ultrasound technologies.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Approach to Examine Cell-Antibody Avidity with Surface Plasmon Resonance Imaging.","authors":"Richard B M Schasfoort, Elise van Doorn, Jos van Weperen, Anouk Mentink, Ruchi Bansal","doi":"10.3390/bios15090559","DOIUrl":"10.3390/bios15090559","url":null,"abstract":"<p><p>In recent years, avidity has emerged as a critical parameter in antibody design, yet most current analytical instruments are limited to measuring affinity alone. This study aims to evaluate the capabilities and advantages of a novel surface plasmon resonance imaging instrument, CellVysion, designed to quantify cell-antibody avidity using a continuous antibody density gradient. A key feature of this approach is the identification of a \"tipping point\"-the specific ligand density, measured in µRIUs, at which cells remain bound to the sensor surface under defined shear flow conditions. In this paper, we present the technical principles and application of this method, demonstrating how avidity can be quantitatively assessed across different antibody-cell line combinations.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467693/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress in Nickel MOF-Based Materials for Electrochemical Biosensor and Supercapacitor Applications.","authors":"Shanmugam Vignesh, Khursheed Ahmad, Tae Hwan Oh","doi":"10.3390/bios15090560","DOIUrl":"10.3390/bios15090560","url":null,"abstract":"<p><p>Nickel-based metal-organic frameworks (Ni-MOFs) have received enormous amounts of attention from the scientific community due to their excellent porosity, larger specific surface area, tunable structure, and intrinsic redox properties. In previous years, Ni-MOFs and their hybrid composite materials have been extensively explored for electrochemical sensing applications. As per the reported literature, Ni-MOF-based hybrid materials have been used in the fabrication of electrochemical sensors for the monitoring of ascorbic acid, glucose, L-tryptophan, bisphenol A, carbendazim, catechol, hydroquinone, 4-chlorophenol, uric acid, kaempferol, adenine, _L-cysteine, etc. The presence of synergistic effects in Ni-MOF-based hybrid materials plays a crucial role in the development of highly selective electrochemical sensors. Thus, Ni-MOF-based materials exhibited enhanced sensitivity and selectivity with reasonable real sample recovery, which suggested their potential for practical applications. In addition, Ni-MOF-based hybrid composites were also adopted as electrode modifiers for the development of supercapacitors. The Ni-MOF-based materials demonstrated excellent specific capacitance at low current densities with reasonable cyclic stability. This review article provides an overview of recent advancements in the utilization of Ni-MOF-based electrode modifiers with metal oxides, carbon-based materials, MXenes, polymers, and LDH, etc., for the electrochemical detection of environmental pollutants and biomolecules and for supercapacitor applications. In addition, Ni-based bimetallic and trimetallic catalysts and their composites have been reviewed for electrochemical sensing and supercapacitor applications. The key challenges, limitations, and future perspectives of Ni-MOF-based materials are discussed. We believe that the present review article may be beneficial for the scientific community working on the development of Ni-MOF-based materials for electrochemical sensing and supercapacitor applications.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthetic Gene Circuits Enable Sensing in Engineered Living Materials.","authors":"Yaxuan Cai, Yujie Wang, Shengbiao Hu","doi":"10.3390/bios15090556","DOIUrl":"10.3390/bios15090556","url":null,"abstract":"<p><p>Engineered living materials (ELMs) integrate living cells-such as bacteria, yeast, or mammalian cells-with synthetic matrices to create responsive, adaptive systems for sensing and actuation. Among ELMs, those endowed with sensing capabilities are gaining increasing attention for applications in environmental monitoring, biomedicine, and smart infrastructure. Central to these sensing functions are synthetic gene circuits, which enable cells to detect and respond to specific signals. This mini-review focuses on recent advances in sensing ELMs empowered by synthetic gene circuits. Here, we highlight how rationally designed genetic circuits enable living materials to sense and respond to diverse inputs-including environmental chemicals, light, heat, and mechanical loadings-via programmable signal transduction and tailored output behaviors. Input signals are classified by their source and physicochemical properties, including synthetic inducers, environmental chemicals, light, thermal, mechanical, and electrical signals. Particular emphasis is placed on the integration of genetically engineered microbial cells with hydrogels and other functional scaffolds to construct robust and tunable sensing platforms. Finally, we discuss the current challenges and future opportunities in this rapidly evolving field, providing insights to guide the rational design of next-generation sensing ELMs.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467368/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-Dimensional SERS Substrates: Architectures, Hot Spot Engineering, and Biosensing Applications.","authors":"Xiaofeng Zhou, Siqiao Liu, Hailang Xiang, Xiwang Li, Chunyan Wang, Yu Wu, Gen Li","doi":"10.3390/bios15090555","DOIUrl":"10.3390/bios15090555","url":null,"abstract":"<p><p>Three-dimensional (3D) surface-enhanced Raman scattering (SERS) substrates have demonstrated remarkable abilities of ultrasensitive and reproducible molecular detection. The combination of both electromagnetic and chemical enhancement processes, light trapping, and multiple scattering effects of 3D structures are what enhance their performance. The principles of underlying enhancements are summarized systematically, and the main types of 3D substrates-vertically aligned nanowires, dendritic and fractal nanostructures, porous frameworks and aerogels, core-shell and hollow nanospheres, and hierarchical hybrid structures-are categorized in this review. Advances in fabrication techniques, such as template-assisted growth, electrochemical and galvanic deposition, dealloying and freeze-drying, self-assembly, and hybrid integration, are critically evaluated in terms of structural tunability and scalability. Novel developments in the field of biosensing are also highlighted, including non-enzymatic glucose sensing, tumor biomarker sensing, and drug delivery. The remaining limitations, such as low reproducibility, mechanical stability, and substrate standardization, are also noted, and future directions, such as stimuli-responsive designs, multifunctional hybrid platforms, and data-driven optimization strategies of SERS technologies, are also included.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sophisticated Interfaces Between Biosensors and Organoids: Advancing Towards Intelligent Multimodal Monitoring Physiological Parameters.","authors":"Yuqi Chen, Shuge Liu, Yating Chen, Miaomiao Wang, Yage Liu, Zhan Qu, Liping Du, Chunsheng Wu","doi":"10.3390/bios15090557","DOIUrl":"10.3390/bios15090557","url":null,"abstract":"<p><p>The integration of organoids with biosensors serves as a miniaturized model of human physiology and diseases, significantly transforming the research frameworks surrounding drug development, toxicity testing, and personalized medicine. This review aims to provide a comprehensive framework for researchers to identify suitable technical approaches and to promote the advancement of organoid sensing towards enhanced biomimicry and intelligence. To this end, several primary methods for technology integration are systematically outlined and compared, which include microfluidic integrated systems, microelectrode array (MEA)-based electrophysiological recording systems, optical sensing systems, mechanical force sensing technologies, field-effect transistor (FET)-based sensing techniques, biohybrid systems based on synthetic biology tools, and label-free technologies, including impedance, surface plasmon resonance (SPR), and mass spectrometry imaging. Through multimodal collaboration such as the combination of MEA for recording electrical signals from cardiac organoids with micropillar arrays for monitoring contractile force, these technologies can overcome the limitations inherent in singular sensing modalities and enable a comprehensive analysis of the dynamic responses of organoids. Furthermore, this review discusses strategies for integrating strategies of multimodal sensing approaches (e.g., the combination of microfluidics with MEA and optical methods) and highlights future challenges related to sensor implantation in vascularized organoids, signal stability during long-term culture, and the standardization of clinical translation.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of the Tolerance of Novel Coronaviruses to Temperature Changes Based on SERS Technology.","authors":"Yusi Peng, Shuai Zhao, Masaki Tanemura, Yong Yang, Ming Liu","doi":"10.3390/bios15090558","DOIUrl":"10.3390/bios15090558","url":null,"abstract":"<p><p>Motivated by the rapid development of SERS technology, trace detection of various viruses in the sewage and body fluid environments and accurate positive and negative diagnosis of detection samples can be achieved. However, evaluating the environmental survival ability of viruses based on SERS technology remains an unexplored issue, but holds significant guiding significance for effective epidemic prevention and control as well as inactivation treatment. In this work, Au nanoarrays were fabricated on silicon substrates through a simple Ar ion sputtering route as ultra-sensitive SERS chips. With the synergistic contribution of the \"lightning rod\" effect and the enhanced coupling surface plasmon caused by the nanoarrays, the ultra-sensitive detection of SARS-CoV-2 S protein with a concentration of 1 pg/mL and SERS enhancement factor of 4.89 × 10⁹ can be achieved. Exploration of the environmental survival ability of the SARS-CoV-2 virus indicates that the Raman activity of SARS-CoV-2 S protein exhibited higher temperature tolerance from 0 °C to 60 °C than SARS-CoV S protein, suggesting that the SARS-CoV-2 virus has less temperature influence from increasing air temperature than the SARS-CoV virus to a certain extent, which explains the seasonal recurrence pattern and regional transmission pattern of the novel coronavirus that are different from the SARS virus.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 9","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467563/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered TtgR-Based Whole-Cell Biosensors for Quantitative and Selective Monitoring of Bioactive Compounds.","authors":"Kyeongseok Song, Haekang Ji, Jiwon Lee, Geupil Jang, Youngdae Yoon","doi":"10.3390/bios15080554","DOIUrl":"https://doi.org/10.3390/bios15080554","url":null,"abstract":"<p><p>TtgR, a transcriptional repressor from <i>Pseudomonas putida</i>, plays a key role in regulating multidrug resistance by controlling the expression of genes in response to various ligands. Despite its broad specificity, TtgR represents a promising candidate for the development of transcription factor (TF)-based biosensors. In this study, we utilized TtgR and its native promoter region (P<i>_ttgABC</i>) as genetic components to construct TF-based biosensors in <i>Escherichia coli</i>. By coupling TtgR and P<i>_ttgABC</i> with <i>egfp</i>, we developed a biosensor responsive to diverse flavonoids. To enhance the selectivity and specificity of the biosensor, we genetically engineered a TtgR-binding pocket. Engineered TtgR variants exhibited altered sensing profiles, enabling the development of biosensors with tailored ligand responses. Computational structural analysis and ligand docking provided insights into the interaction mechanisms between TtgR variants and flavonoids. Notably, biosensors based on wild-type TtgR and its N110F mutant were capable of quantifying resveratrol and quercetin at 0.01 mM with >90% accuracy. Although the precise molecular mechanisms involved remain unclear and further optimization is needed, the biosensors developed herein demonstrate strong potential for applications in numerous fields. This study lays the foundation for future research that could extend the utility of TtgR-based biosensors to synthetic biology, metabolic engineering, and beyond.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 8","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12384351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144975074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Sensitive Recombinase-Aided Amplification Integrated Test Strip for <i>Pseudomonas fluorescens</i> in Milk via Dual Gene Probes.","authors":"Guangying Zhang, Lili Zhang, Jingqin Ye, Dongshu Wang, Ying Lu","doi":"10.3390/bios15080553","DOIUrl":"https://doi.org/10.3390/bios15080553","url":null,"abstract":"<p><p><i>Pseudomonas fluorescens</i> is the main spoilage bacterium in milk, and its proliferation is one of the factors leading to the deterioration of the quality of raw milk. In this study, a rapid detection system for <i>P. fluorescens</i> was developed based on recombinase-aided amplification combined with a test strip (RAA-TS), which contained a double test line (DTL) targeting the virulence gene <i>aprX</i> of <i>P. fluorescens</i> and the housekeeping gene <i>gyrB</i> of <i>Pseudomonas</i>. Visual observation could detect <i>gyrB</i> (50 CFU/mL) and <i>aprX</i> (250 CFU/mL) within 90 min, including sample pretreatment and RAA reaction and detection steps. No cross-reactions were observed with <i>Pseudomonas</i> or other bacteria (n = 19). The quantitative detection limits (LOD) of <i>gyrB</i> and <i>aprX</i> for <i>P. fluorescens</i> in milk were 37 CFU/mL and 233 CFU/mL, respectively. Compared with polymerase chain reaction-agarose gel electrophoresis (PCR-AGE), the sensitivity of the developed RAA-TS-DTL system was increased by approximately four times. Furthermore, it could detect live <i>P. fluorescens</i> in milk when combined with optimized sample pretreatment by propidium monoazide (PMAxx). Its consistency with the traditional culture method in the detection of <i>P. fluorescens</i> spiked in milk samples (n = 25) was 100%. The developed RAA-TS-DTL had the advantages of high accuracy and short time consumption. Thus, it provides a new way or tool for the rapid screening or detection of <i>P. fluorescens</i> in milk.</p>","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 8","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12385110/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144975067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}