Biosensors-Basel最新文献_第7页

Cellular Mechanical Phenotypes of Drought-Resistant and Drought-Sensitive Rice Species Distinguished by Double-Resonator Piezoelectric Cytometry Biosensors. 利用双谐振压电细胞仪生物传感器识别抗旱和抗旱水稻品种的细胞力学表型。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-23 DOI: 10.3390/bios15060334

Ding Tang, Tiean Zhou, Weisong Pan, Shimei Wang, Muhammad Ahmad Hassan

{"title":"Cellular Mechanical Phenotypes of Drought-Resistant and Drought-Sensitive Rice Species Distinguished by Double-Resonator Piezoelectric Cytometry Biosensors.","authors":"Ding Tang, Tiean Zhou, Weisong Pan, Shimei Wang, Muhammad Ahmad Hassan","doi":"10.3390/bios15060334","DOIUrl":"10.3390/bios15060334","url":null,"abstract":"Various high-throughput screening methods have been developed to explore plant phenotypes, primarily at the organ and whole plant levels. There is a need to develop phenomics methods at the cellular level to narrow down the genotype to phenotype gap. This study used double-resonator piezoelectric cytometry biosensors to capture the dynamic changes in mechanical phenotypes of living cells of two rice species, drought-resistant Lvhan No. 1 and drought-sensitive 6527, under PEG6000 drought stress. In rice cells of Lvhan No. 1 and 6527, mechanomics parameters, including cell-generated surface stress (ΔS) and viscoelastic parameters (G', G″, G″/G'), were measured and compared under 5-25% PEG6000. Lvhan No. 1 showed larger viscoelastic but smaller surface stress changes with the same concentration of PEG6000. Moreover, Lvhan No. 1 cells showed better wall-plasma membrane-cytoskeleton continuum structure maintaining ability under drought stress, as proven by transient tension stress (ΔS > 0) and linear G'~ΔS, G″~ΔS relations at higher 15-25% PEG6000, but not for 6527 cells. Additionally, two distinct defense and drought resistance mechanisms were identified through dynamic G″/G' responses: (i) transient hardening followed by softening recovery under weak drought, and (ii) transient softening followed by hardening recovery under strong drought. The abilities of Lvhan No. 1 cells to both recover from transient hardening to softening and to recover from transient softening to hardening are better than those of 6527 cells. Overall, the dynamic mechanomics phenotypic patterns (ΔS, G', G″, G″/G', G'~ΔS, G″~ΔS) verified that Lvhan No. 1 has better drought resistance than that of 6527, which is consistent with the field data.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 6","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12191338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Progress on Rare-Earth-Doped Upconversion Nanomaterials for Bioassay Applications. 稀土掺杂上转换纳米生物检测材料研究进展。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-23 DOI: 10.3390/bios15060335

Jiling Xu, Hengyuan Cao, Chenwei Wu, Ting Wang, Liheng Sun, Biao Dong

引用次数: 0

Ultrasensitive Analysis of BRCA-1 Based on Gold Nanoparticles and Molybdenum Disulfide Electrochemical Immunosensor with Enhanced Signal Amplification. 基于金纳米粒子和二硫化钼电化学免疫传感器增强信号放大的BRCA-1超灵敏分析。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-21 DOI: 10.3390/bios15050330

Derya Bal Altuntaş

{"title":"Ultrasensitive Analysis of BRCA-1 Based on Gold Nanoparticles and Molybdenum Disulfide Electrochemical Immunosensor with Enhanced Signal Amplification.","authors":"Derya Bal Altuntaş","doi":"10.3390/bios15050330","DOIUrl":"10.3390/bios15050330","url":null,"abstract":"The BRCA-1 protein, recognized for its diagnostic relevance in a wide spectrum of malignancies, has been the focus of extensive investigation. In this study, an electrochemical immunosensor specifically designed for BRCA-1 detection was fabricated. The sensing platform utilizes disposable pencil graphite electrodes modified with a nanocomposite composed of gold nanoparticles (AuNPs), molybdenum disulfide (MoS2), and chitosan (CS). This multifunctional nanostructure significantly promotes electron transfer efficiency and supports the effective immobilization of antibodies. The constructed immunosensor exhibited excellent analytical performance, with a linear detection range between 0.05 and 20 ng/mL for BRCA-1 and a notably low limit of detection at 0.04 ng/mL. The device maintained a relative standard deviation of 3.59% (n = 3), indicating strong reproducibility. In addition, a high recovery rate of 98 ± 3% was achieved in spiked serum samples, even in the presence of common electroactive interferents such as dopamine and ascorbic acid. These findings highlight the sensor's promising applicability for the clinical detection of BRCA-1 and potentially other cancer-related biomarkers.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109648/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probing the Influence of Specular Reflection and Overexposure on Backscattering Mueller Matrix Polarimetry for Tissue Imaging and Sensing. 探究镜面反射和过度曝光对后向散射穆勒矩阵偏振法用于组织成像和传感的影响。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-21 DOI: 10.3390/bios15050333

Wei Jiao, Nan Zeng, Rui Hao, Hui Ma, Chao He, Honghui He

{"title":"Probing the Influence of Specular Reflection and Overexposure on Backscattering Mueller Matrix Polarimetry for Tissue Imaging and Sensing.","authors":"Wei Jiao, Nan Zeng, Rui Hao, Hui Ma, Chao He, Honghui He","doi":"10.3390/bios15050333","DOIUrl":"10.3390/bios15050333","url":null,"abstract":"Mueller matrix polarimetry has great potential for tissue detection and clinical diagnosis due to its ability to provide rich microstructural information accurately. However, in practical in vivo tissue imaging based on backscattering Mueller matrix polarimetry, specular reflection is often inevitable, leading to overexposed regions and the following inaccurate polarization information acquisition of tissues. In this study, we probe the influence of specular reflection and overexposure on backscattering Mueller matrix polarimetry for tissue imaging and sensing. We investigate in detail the differentiation of polarization behaviors between the specular reflection and non-specular reflection tissue regions using a 3 × 3 backscattering Mueller matrix measurement. Then, we obtain the vertical projection profiles to further quantify the Mueller matrix elements of porcine liver tissue in different specular reflection regions. Finally, we calculate the polarization feature parameters derived from a 3 × 3 Mueller matrix and analyze their behavior in overexposed regions. Based on the quantitative analysis and comparisons, we obtain a group of polarization feature parameters with strong immunity to the specular reflection process. This study offers a strategy for selecting the polarization parameters during in vivo polarimetric imaging applications, provides valuable references for further eliminating the characterization errors induced by specular reflection, and may contribute to the advancement of quantitative tissue polarimetric imaging and sensing.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12110177/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silver Nanoparticles for Biosensing and Drug Delivery: A Mechanical Study on DNA Interaction. 用于生物传感和药物传递的纳米银：DNA相互作用的力学研究。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-21 DOI: 10.3390/bios15050331

Katarína Nemčeková, Patrícia Dudoňová, Tomáš Holka, Sabína Balážová, Michaela Hornychová, Viktória Szebellaiová, Monika Naumowicz, Pavol Gemeiner, Tomáš Mackuľak, Miroslav Gál, Veronika Svitková

{"title":"Silver Nanoparticles for Biosensing and Drug Delivery: A Mechanical Study on DNA Interaction.","authors":"Katarína Nemčeková, Patrícia Dudoňová, Tomáš Holka, Sabína Balážová, Michaela Hornychová, Viktória Szebellaiová, Monika Naumowicz, Pavol Gemeiner, Tomáš Mackuľak, Miroslav Gál, Veronika Svitková","doi":"10.3390/bios15050331","DOIUrl":"10.3390/bios15050331","url":null,"abstract":"Silver nanoparticles (AgNPs) have attracted tremendous attention in recent years due to their unique physicochemical properties, including pronounced surface plasmon resonance, tunable size, and amenability to functionalization. These attributes underpin the growing interest in AgNPs as SMART nanocarriers for targeted drug delivery and as active components in biosensing platforms. In this work, we discuss various synthesis strategies for AgNPs-ranging from conventional chemical methods to green approaches-and highlight their subsequent functionalization with anticancer drugs, notably doxorubicin (DOX). We also examine the potential of AgNPs in biosensor applications, emphasizing electrochemical and optical detection modalities capable of monitoring drug release, oxidative stress, and relevant biomarkers. Our experimental data support the conclusion that AgNPs can effectively improve therapeutic efficacy by exploiting tumor-specific conditions (e.g., lower pH) while also enhancing biosensor sensitivity via surface plasmon resonance and electrochemical signal amplification. We provide a thorough discussion of the results, including mechanistic aspects of reactive oxygen species (ROS) generation, drug release kinetics, and sensor performance metrics. Overall, AgNP-based nanocarriers emerge as a powerful platform to address current challenges in precision oncology and medical diagnostics.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12110216/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzyme-Based Solid-Phase Electrochemiluminescence Sensors with Stable, Anchored Emitters for Sensitive Glucose Detection. 基于酶的固相电化学发光传感器，稳定，锚定发射器用于敏感的葡萄糖检测。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-21 DOI: 10.3390/bios15050332

Chunyin Wei, Yanyan Zheng, Fei Yan, Lifang Xu

{"title":"Enzyme-Based Solid-Phase Electrochemiluminescence Sensors with Stable, Anchored Emitters for Sensitive Glucose Detection.","authors":"Chunyin Wei, Yanyan Zheng, Fei Yan, Lifang Xu","doi":"10.3390/bios15050332","DOIUrl":"10.3390/bios15050332","url":null,"abstract":"Glucose (Glu) detection, as a fundamental analytical technique, has applications in medical diagnostics, clinical testing, bioanalysis and environmental monitoring. In this work, a solid-phase electrochemiluminescence (ECL) enzyme sensor was developed by immobilizing the ECL emitter in a stable manner within bipolar silica nanochannel array film (bp-SNA), enabling sensitive glucose detection. The sensor was constructed using an electrochemical-assisted self-assembly (EASA) method with various siloxane precursors to quickly modify the surface of indium tin oxide (ITO) electrodes with a bilayer SNA of different charge properties. The inner layer, including negatively charged SNA (n-SNA), attracted the positively charged ECL emitter tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)32+) via electrostatic interaction, while the outer layer, including positively charged SNA (p-SNA), repelled it, forming a barrier that efficiently concentrated the Ru(bpy)32+ emitter in a stable manner. After modifying the amine groups on the p-SNA surface with aldehyde groups, glucose oxidase (GOx) was covalently immobilized, forming the enzyme electrode. In the presence of glucose, GOx catalyzed the conversion of glucose to hydrogen peroxide (H2O2), which acted as a quencher for the Ru(bpy)32+/triethanolamine (TPA) system, reducing the ECL signal and enabling quantitative glucose analysis. The sensor exhibited a wide linear range from 10 μM to 7.0 mM and a limit of detection (LOD) of 1 μM (S/N = 3). Glucose detection in fetal bovine serum was realized. By replacing the enzyme type on the electrode surface, this sensing strategy holds the potential to provide a universal platform for the detection of different metabolites.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strain Plethysmography Using a Hermetically Sealed MEMS Strain Sensor. 使用密闭式MEMS应变传感器进行应变体积描记。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-20 DOI: 10.3390/bios15050325

Xinyu Jiang, Brian Sang, Haoran Wen, Gregory Junek, Jin-Woo Park, Farrokh Ayazi

{"title":"Strain Plethysmography Using a Hermetically Sealed MEMS Strain Sensor.","authors":"Xinyu Jiang, Brian Sang, Haoran Wen, Gregory Junek, Jin-Woo Park, Farrokh Ayazi","doi":"10.3390/bios15050325","DOIUrl":"10.3390/bios15050325","url":null,"abstract":"We present a hermetically sealed capacitive microelectromechanical system (MEMS) strain sensor designed for arterial pulse waveform extraction using the strain plethysmography (SPG) modality. The MEMS strain sensor features a small form factor of 3.3 mm × 3.3 mm × 1 mm, leverages a nano-gap fabrication process to improve the sensitivity, and uses a differential sensing mechanism to improve the linearity and remove the common mode drift. The MEMS strain sensor is interfaced with an application-specific integrated circuit (ASIC) to form a compact strain sensing system. This system exhibits a high strain sensitivity of 316 aF/µε, a gauge factor (GF) of 35, and a strain sensing resolution of 1.26 µε, while maintaining a linear range exceeding 700 µε. SPG signals have been reliably captured at both the fingertip and wrist using the MEMS strain sensor with high signal quality, preserving various photoplethysmography (PPG) features. Experimental results demonstrate that heart rate (HR) and heart rate variability (HRV) can be estimated from the SPG signal collected at the fingertip and wrist using the sensor with an accuracy of over 99%. Pulse arrival time (PAT) and pulse transit time (PTT) have been successfully extracted using the sensor together with a MEMS seismometer, showcasing its potential for ambulatory BP monitoring (ABPM) application.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12110129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Convenient Biochemical Testing Technologies for Oral Disease Risk Warning: Opportunities and Challenges. 便捷的口腔疾病风险预警生化检测技术：机遇与挑战。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-20 DOI: 10.3390/bios15050327

Ying Liu, Jincheng Xu, Siyuan Wang, Yuanfang Li, Li Ji, Dong Xie, Jianhua Zhou

{"title":"Convenient Biochemical Testing Technologies for Oral Disease Risk Warning: Opportunities and Challenges.","authors":"Ying Liu, Jincheng Xu, Siyuan Wang, Yuanfang Li, Li Ji, Dong Xie, Jianhua Zhou","doi":"10.3390/bios15050327","DOIUrl":"10.3390/bios15050327","url":null,"abstract":"In recent years, attention toward oral health issues has increased with economic development and improvements in quality of life. Biochemical testing technologies offer an efficient method for identifying insidious pathological changes in the oral cavity. Frequent home-based self-screening can enable early identification of dental disease risks, thus facilitating timely interventions. Convenient home-based biochemical testing methods must be user-friendly, cost-effective, and operable without specialized equipment or extensive training. This review summarizes recent advances in convenient biochemical testing methods for the detection and diagnosis of oral diseases, focusing on their reliability, user compliance, and practicality for home-based applications. This review highlights the significance of biomarker distribution imaging for simultaneously identifying multiple lesions and provides perspectives on future research directions. By promoting interdisciplinary collaboration in biochemical diagnostics, this review outlines pathways toward personalized oral healthcare, precision dentistry, and enhanced overall health outcomes.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the Possibility of Fluorescent Capture Immunoassays on a Contact Lens. 隐形眼镜荧光捕获免疫分析的可能性。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-20 DOI: 10.3390/bios15050326

Kundan Sivashanmugan, E Albert Reece, Joseph R Lakowicz

{"title":"On the Possibility of Fluorescent Capture Immunoassays on a Contact Lens.","authors":"Kundan Sivashanmugan, E Albert Reece, Joseph R Lakowicz","doi":"10.3390/bios15050326","DOIUrl":"10.3390/bios15050326","url":null,"abstract":"Blood samples and testing are routine in healthcare. Presently, there is a growing interest in using tear samples in place of blood. Tear samples can be obtained non-invasively and collection does not require the skills of a trained phlebotomist. Red blood cells and other cells are not present in tears, which avoids centrifugation. Importantly, basal tear samples contain most of the biomarkers present in blood. The difficulty is the small volume of basal tears, which is about 7 μL in each eye. Any contact with the eye results in additional reflex tears with a different chemical composition. The small tear samples are collected with capillary tubes and then sent out for amplified assays, such as enzyme-linked immunosorbent assay (ELISA) or polymerase chain reaction (PCR). The results are not available for several days or a week and, therefore, are less useful in an ophthalmology office. We propose the use of a contact lens that contains bound antibodies for fluorescence immunoassays. The lenses could be removed from the patient for point-of-care measurements at the bedside. To prove that this concept is possible, we performed a three-layer protein capture assay that mimics an immunoassay. For convenience, we used lysozyme (Lys), which spontaneously coats silicon hydrogel (SiHG) contact lenses (CL). Anti-lysozyme IgG was the second layer captured, with anti-lysozyme considered to be the target biomarker. The third layer was rhodamine or Alexa Fluor-labeled Ab against the IgG Fc region, considered to be the detection antibody. The multiple protein layers were stable and did not wash off the SiHG lenses. These results strongly suggest the contact lens can be used for capture immunoassays for a wide variety of biomarkers.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12110756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Biomodification and Biomimetic Synthesis of 2D Nanomaterial-Based Nanohybrids for Biosensor Applications: A Review. 基于二维纳米材料的生物修饰和仿生合成在生物传感器中的应用综述。

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-05-20 DOI: 10.3390/bios15050328

Ranran Wang, Xinyue Wang, Yan Wang, Gang Wei

{"title":"The Biomodification and Biomimetic Synthesis of 2D Nanomaterial-Based Nanohybrids for Biosensor Applications: A Review.","authors":"Ranran Wang, Xinyue Wang, Yan Wang, Gang Wei","doi":"10.3390/bios15050328","DOIUrl":"10.3390/bios15050328","url":null,"abstract":"Two-dimensional nanomaterials (2DNMs) exhibit significant potential for the development of functional and specifically targeted biosensors, owing to their unique planar nanosheet structures and distinct physical and chemical properties. Biomodification and biomimetic synthesis offer green and mild approaches for the fabrication of multifunctional nanohybrids with enhanced catalytic, fluorescent, electronic, and optical properties, thereby expanding their utility in constructing high-performance biosensors. In this review, we present recent advances in the synthesis of 2DNM-based nanohybrids via both biomodification and biomimetic strategies for biosensor applications. We discuss covalent and non-covalent biomodification methods involving various biomolecules, including peptides, proteins, DNA/RNA, enzymes, biopolymers, and bioactive polysaccharides. The engineering of biomolecule-nanomaterial interfaces for the creation of biomodified 2DNM-based nanohybrids is also explored. Furthermore, we summarize the biomimetic synthesis of 2DNM-based bio-nanohybrids through pathways such as bio-templating, biomolecule-directed self-assembly, biomineralization, and biomimetic functional integration. The potential applications of these nanohybrids in diverse biosensing platforms-including colorimetric, surface plasmon resonance, electrochemical, fluorescence, photoelectrochemical, and integrated multimodal biosensors-are introduced and discussed. Finally, we analyze the opportunities and challenges associated with this rapidly developing field. We believe this comprehensive review will provide valuable insights into the biofunctionalization of 2DNMs and guide the rational design of advanced biosensors for diagnostic applications.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12110365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0