Biosensors-Basel最新文献_第7页

On the Feasibility of SERS-Based Monitoring of Drug Loading Efficiency in Exosomes for Targeted Delivery.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-23 DOI: 10.3390/bios15030141

Jun Liu, Siddharth Srivastava, Tieyi Li, Faycal Moujane, John Y Lee, Yiqing Chen, Huinan Liu, Sophie X Deng, Ya-Hong Xie

{"title":"On the Feasibility of SERS-Based Monitoring of Drug Loading Efficiency in Exosomes for Targeted Delivery.","authors":"Jun Liu, Siddharth Srivastava, Tieyi Li, Faycal Moujane, John Y Lee, Yiqing Chen, Huinan Liu, Sophie X Deng, Ya-Hong Xie","doi":"10.3390/bios15030141","DOIUrl":"10.3390/bios15030141","url":null,"abstract":"Cancer, a significant cause of mortality, necessitates improved drug delivery strategies. Exosomes, as natural drug carriers, offer a more efficient, targeted, and less toxic drug delivery system compared to direct dispersal methods via ingestion or injection. To be successfully implemented as drug carriers, efficient loading of drugs into exosomes is crucial, and a deeper understanding of the loading mechanism remains to be solved. This study introduces surface-enhanced Raman scattering (SERS) to monitor drug loading efficacy at the single vesicle level. By enhancing the Raman signal, SERS overcomes limitations in Raman spectroscopy. A gold nanopyramids array-based SERS substrate assesses exosome heterogeneity in drug-loading capabilities with the help of single-layer graphene for precise quantification. This research advances targeted drug delivery by presenting a more efficient method of evaluating drug-loading efficiency into individual exosomes through SERS-based monitoring. Furthermore, the study explores leveraging osmotic pressure variations, enhancing the efficiency of drug loading into exosomes.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 3","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939968/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aptamer-Based Graphene Field-Effect Transistor Biosensor for Cytokine Detection in Undiluted Physiological Media for Cervical Carcinoma Diagnosis.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-23 DOI: 10.3390/bios15030138

Ziran Wang, Wenting Dai, Zaiyu Zhang, Haipeng Wang

{"title":"Aptamer-Based Graphene Field-Effect Transistor Biosensor for Cytokine Detection in Undiluted Physiological Media for Cervical Carcinoma Diagnosis.","authors":"Ziran Wang, Wenting Dai, Zaiyu Zhang, Haipeng Wang","doi":"10.3390/bios15030138","DOIUrl":"10.3390/bios15030138","url":null,"abstract":"Personalized monitoring of disease biomarkers is of great interest in women's health. However, existing approaches typically involve invasive inspection or bulky equipment, making them challenging to implement at home. Hence, we present a general strategy for label-free and specific detection of disease biomarkers in physiological media using an aptamer-based biosensor. The biosensor is a graphene field-effect transistor that involves immobilizing the aptamer and a biomolecule-permeable polyethylene glycol (PEG) layer on the graphene surface. The aptamer is capable of specifically binding with the target biomarker, thus inducing a change in the sensing responses. The PEG layer can effectively reduce the nonspecific adsorption of nontarget molecules in the solution, and increase the effective Debye screening length in the region directly adjacent to the graphene. In this work, studies of a biosensor with modification of the aptamer and PEG show that cervical carcinoma biomarkers such as tumor necrosis factor-α and interleukin 6 can be sensitively and specifically detected in undiluted physiological media, with detection limits as low as 0.13 pM for TNF-a and 0.20 pM for IL-6. This work presents a significant method for the general application of the biosensor for disease diagnosis in women's health.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 3","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Bulk Planar Junctionless Field-Effect Transistor for High-Performance Biosensing.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-22 DOI: 10.3390/bios15030135

Jeongmin Son, Chan Heo, Hyeongyu Kim, M Meyyappan, Kihyun Kim

{"title":"A Novel Bulk Planar Junctionless Field-Effect Transistor for High-Performance Biosensing.","authors":"Jeongmin Son, Chan Heo, Hyeongyu Kim, M Meyyappan, Kihyun Kim","doi":"10.3390/bios15030135","DOIUrl":"10.3390/bios15030135","url":null,"abstract":"Biologically sensitive field-effect transistors (BioFETs) have advanced the biosensing capabilities in various fields such as healthcare, security and environmental monitoring. Here, we propose a junctionless BioFET (JL-BioFET) for the high-sensitivity and low-cost detection of biomolecules and analyze it using detailed device simulations. In contrast to the conventional FET with junctions, the JL-BioFET simplifies fabrication by doping the source, channel and drain simultaneously with the same types of impurities, thereby reducing the fabrication effort and cost. Additionally, if the device is designed with optimal bias, it can operate with only the source and drain terminals, which reduces power consumption. Thus, cost reduction and reduced power consumption are strong motivations to pursue a new design. Therefore, we simulated two JL-BioFET structures (SOI JL, bulk JL) that operate without a gate electrode and compared their biosensing performances. The bulk JL-BioFET showed an average sensitivity three times higher than that of the SOI JL-BioFET across varying charge levels. Then, we optimized the sensing performance of the bulk JL-BioFET by adjusting three key parameters: the active layer thickness and the doping concentrations of the active layer and substrate. These encouraging results are expected to lead to future fabrication efforts to realize bulk JL-BioFETs for high-performance biosensing.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 3","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11940246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-Channel Colloidal Gold-Based Immunochromatographic Test Strip for Rapidly Differentiating Between Two Major Groups of Paracidovorax citrulli.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-22 DOI: 10.3390/bios15030133

Ling Sun, Yuanfei Xing, Xin Yang, Yanli Tian, Wenyao Zhang, Chen Zhang, Rui Fan, Weirong Gong, Jie Hu, Xiaolong Shao, Guoliang Qian, Baishi Hu, Limin Wang

{"title":"Dual-Channel Colloidal Gold-Based Immunochromatographic Test Strip for Rapidly Differentiating Between Two Major Groups of Paracidovorax citrulli.","authors":"Ling Sun, Yuanfei Xing, Xin Yang, Yanli Tian, Wenyao Zhang, Chen Zhang, Rui Fan, Weirong Gong, Jie Hu, Xiaolong Shao, Guoliang Qian, Baishi Hu, Limin Wang","doi":"10.3390/bios15030133","DOIUrl":"10.3390/bios15030133","url":null,"abstract":"Bacterial fruit blotch (BFB) caused by Paracidovorax citrulli is a severe threat to melon, watermelon, and other cucurbit crop production worldwide. The long-term adaptation of the pathogen to environmental conditions has resulted in substantial genetic diversity. In this study, we used P. citrulli strains from two groups as immunogens to obtain antibodies that were used to generate A.C1 and A.C2 colloidal gold immunochromatographic single test strips, which specifically identified group I and group II P. citrulli strains, respectively. We combined the A.C1 and A.C2 single test strips in a dual-channel plastic cartridge to construct a dual-channel colloidal gold immunochromatographic test strip able to distinguish between P. citrulli strains from two distinct groups. Test strip sensitivity reached 106 CFU/mL under ideal conditions. Moreover, it was relatively stable, with no cross-reactions with strains of closely related genera. The dual-channel test strip developed in this study may provide farmers with a useful tool for managing BFB through the prompt implementation of quarantine procedures in the field.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 3","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11940258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging Dual-Gate FET Sensor Paradigm for Ultra-Low Concentration Cortisol Detection in Complex Bioenvironments.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-22 DOI: 10.3390/bios15030134

Seung-Jin Lee, Won-Ju Cho

{"title":"Emerging Dual-Gate FET Sensor Paradigm for Ultra-Low Concentration Cortisol Detection in Complex Bioenvironments.","authors":"Seung-Jin Lee, Won-Ju Cho","doi":"10.3390/bios15030134","DOIUrl":"10.3390/bios15030134","url":null,"abstract":"Cortisol is a pivotal hormone regulating stress responses and is linked to various health conditions, making precise and continuous monitoring essential. Despite their non-invasive nature, conventional cortisol detection methods often suffer from inadequate sensitivity and reliability at low concentrations, limiting their diagnostic utility. To address these limitations, this study introduces a novel paradigm for high sensitivity cortisol detection using field-effect transistor (FET) sensors with dual-gate (DG) structures. The proposed sensor platform enhances sensitivity through capacitive coupling without requiring external circuits. Cortisol detection performance was evaluated by immobilizing monoclonal antibodies activated via 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and N-hydroxysuccinimide onto a SnO2 thin film-based extended-gate. The results revealed a sensitivity of 14.3 mV/dec in single-gate mode, which significantly increased to 243.8 mV/dec in DG mode, achieving a detection limit of 276 pM. Additionally, the reliability and stability of the sensor were validated by evaluating drift effects, confirming its ability to provide accurate detection even in artificial saliva environments containing interfering substances. In conclusion, the DG-FET-based cortisol detection approach developed in this study significantly outperforms conventional FET-based methods, enabling precise monitoring at ultra-low concentrations. This approach holds significant potential for diverse bioassays requiring high sensitivity and reliability in complex environments.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 3","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11940039/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards Rapid and Low-Cost Stroke Detection Using SERS and Machine Learning.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-22 DOI: 10.3390/bios15030136

Cristina Freitas, João Eleutério, Gabriela Soares, Maria Enea, Daniela Nunes, Elvira Fortunato, Rodrigo Martins, Hugo Águas, Eulália Pereira, Helena L A Vieira, Lúcio Studer Ferreira, Ricardo Franco

{"title":"Towards Rapid and Low-Cost Stroke Detection Using SERS and Machine Learning.","authors":"Cristina Freitas, João Eleutério, Gabriela Soares, Maria Enea, Daniela Nunes, Elvira Fortunato, Rodrigo Martins, Hugo Águas, Eulália Pereira, Helena L A Vieira, Lúcio Studer Ferreira, Ricardo Franco","doi":"10.3390/bios15030136","DOIUrl":"10.3390/bios15030136","url":null,"abstract":"Stroke affects approximately 12 million individuals annually, necessitating swift diagnosis to avert fatal outcomes. Current hospital imaging protocols often delay treatment, underscoring the need for portable diagnostic solutions. We have investigated silver nanostars (AgNS) incubated with human plasma, deposited on a simple aluminum foil substrate, and utilizing Surface-Enhanced Raman Spectroscopy (SERS) combined with machine learning (ML) to provide a proof-of-concept for rapid differentiation of stroke types. These are the seminal steps for the development of low-cost pre-hospital diagnostics at point-of-care, with potential for improving patient outcomes. The proposed SERS assay aims to classify plasma from stroke patients, differentiating hemorrhagic from ischemic stroke. Silver nanostars were incubated with plasma and spiked with glial fibrillary acidic protein (GFAP), a biomarker elevated in hemorrhagic stroke. SERS spectra were analyzed using ML to distinguish between hemorrhagic and ischemic stroke, mimicked by different concentrations of GFAP. Key innovations include optimized AgNS-plasma incubates formation, controlled plasma-to-AgNS ratios, and a low-cost aluminum foil substrate, enabling results within 15 min. Differential analysis revealed stroke-specific protein profiles, while ML improved classification accuracy through ensemble modeling and feature engineering. The integrated ML model achieved rapid and precise stroke predictions within seconds, demonstrating the assay's potential for immediate clinical decision-making.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 3","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11940671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic Biosensors for the Detection of Motile Plant Zoospores.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-21 DOI: 10.3390/bios15030131

Peikai Zhang, David E Williams, Logan Stephens, Robert Helps, Irene Patricia Shamini Pushparajah, Jadranka Travas-Sejdic, Marion Wood

{"title":"Microfluidic Biosensors for the Detection of Motile Plant Zoospores.","authors":"Peikai Zhang, David E Williams, Logan Stephens, Robert Helps, Irene Patricia Shamini Pushparajah, Jadranka Travas-Sejdic, Marion Wood","doi":"10.3390/bios15030131","DOIUrl":"10.3390/bios15030131","url":null,"abstract":"Plant pathogen zoospores play a vital role in the transmission of several significant plant diseases, with their early detection being important for effective pathogen management. Current methods for pathogen detection involve labour-intensive specimen collection and laboratory testing, lacking real-time feedback capabilities. Methods that can be deployed in the field and remotely addressed are required. In this study, we have developed an innovative zoospore-sensing device by combining a microfluidic sampling system with a microfluidic cytometer and incorporating a chemotactic response as a means to selectively detect motile spores. Spores of Phytophthora cactorum were guided to swim up a detection channel following a gradient of attractant. They were then detected by a transient change in impedance when they passed between a pair of electrodes. Single-zoospore detection was demonstrated with signal-to-noise ratios of ~17 when a carrying flow was used and ~5.9 when the zoospores were induced to swim into the channel following the gradient of the attractants. This work provides an innovative solution for the selective, sensitive and real-time detection of motile zoospores. It has great potential to be further developed into a portable, remotely addressable, low-cost sensing system, offering an important tool for field pathogen real-time detection applications.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 3","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11940179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Lead Halide Perovskite and Copper Iodide Materials for Fluorescence Sensing of Oxygen.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-21 DOI: 10.3390/bios15030132

Jingwen Jin, Yaning Huang, Chen Zhang, Li Zhang, Shaoxing Jiang, Xi Chen

{"title":"Novel Lead Halide Perovskite and Copper Iodide Materials for Fluorescence Sensing of Oxygen.","authors":"Jingwen Jin, Yaning Huang, Chen Zhang, Li Zhang, Shaoxing Jiang, Xi Chen","doi":"10.3390/bios15030132","DOIUrl":"10.3390/bios15030132","url":null,"abstract":"The most commonly used optical oxygen sensing materials are phosphorescent molecules and functionalized nanocrystals. Many exploration studies on oxygen sensing have been carried out using the fluorescence or phosphorescence of semiconductor nanomaterials. Lead halide perovskite nanocrystals, a new type of ionic semiconductor, have excellent optical properties, making them suitable for use in optoelectronic devices. They also show promising applications in analytical sensing and biological imaging, especially manganese-doped perovskite nanocrystals for optical oxygen sensing. As a class of materials with diverse sources, copper iodide cluster semiconductors have rich structural and excellent luminescent properties, and have attracted attention in recent years. These materials have adjustable optical properties and sensitive stimulus response properties, showing great potential for optical sensing applications. This review paper provides a brief introduction to traditional oxygen sensing using organic molecules and introduces research on oxygen sensing using novel luminescent semiconductor materials, perovskite metal halides and copper iodide hybrid materials in recent years. It focuses on the mechanism and application of these materials for oxygen sensing and evaluates the future development direction of these materials for oxygen sensing.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 3","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11940331/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensing Microorganisms Using Rapid Detection Methods: Supramolecular Approaches.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-21 DOI: 10.3390/bios15030130

Hiya Lahiri, Kingshuk Basu

引用次数: 0

Detecting Respiratory Pathogens for Diagnosing Lower Respiratory Tract Infections at the Point of Care: Challenges and Opportunities.

IF 4.9 3区工程技术

Biosensors-Basel Pub Date : 2025-02-20 DOI: 10.3390/bios15030129

Francisco M Bouzada, Bartomeu Mestre, Andreu Vaquer, Sofía Tejada, Roberto de la Rica

{"title":"Detecting Respiratory Pathogens for Diagnosing Lower Respiratory Tract Infections at the Point of Care: Challenges and Opportunities.","authors":"Francisco M Bouzada, Bartomeu Mestre, Andreu Vaquer, Sofía Tejada, Roberto de la Rica","doi":"10.3390/bios15030129","DOIUrl":"10.3390/bios15030129","url":null,"abstract":"Lower respiratory tract infections (LRTIs) are a leading cause of mortality worldwide, claiming millions of lives each year and imposing significant healthcare costs. Accurate detection of respiratory pathogens is essential for the effective management of LRTIs. However, this process often relies on sputum analysis, which requires extensive pretreatment steps. The viscous nature and complex composition of sputum present additional challenges, especially in settings where a rapid diagnosis at the point of care is essential. In this review, we describe the main types of LRTI, highlighting different patient care pathway and points of care. We review current methods for liquefying sputum samples and provide an overview of current commercially available diagnostic tools used in hospitals for LRTI detection. Furthermore, we critically review recent advancements in the literature focused on detecting respiratory pathogens and mechanisms of antimicrobial resistance in sputum, including nucleic acid amplification tests, immunoassays and other innovative approaches. Throughout the paper, we highlight challenges and opportunities associated with developing new biosensor technologies tailored for detecting respiratory pathogens in lower respiratory specimens. By shedding light on these pressing issues, we aim to inspire scientific community to create innovative diagnostic tools to address the urgent healthcare burden of lung diseases.","PeriodicalId":48608,"journal":{"name":"Biosensors-Basel","volume":"15 3","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11940763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0