Journal of Insects as Food and Feed最新文献_第3页

Fatty acid profile of black soldier fly larvae and frass as affected by different growing substrates 不同生长基质对黑实蝇幼虫和虫体脂肪酸谱的影响

IF 5.1 3区农林科学

Journal of Insects as Food and Feed Pub Date : 2024-01-22 DOI: 10.1163/23524588-00001059

N. F. Addeo, I. Tucciarone, F. Bovera, S. Vozzo, G. Secci, G. Parisi

{"title":"Fatty acid profile of black soldier fly larvae and frass as affected by different growing substrates","authors":"N. F. Addeo, I. Tucciarone, F. Bovera, S. Vozzo, G. Secci, G. Parisi","doi":"10.1163/23524588-00001059","DOIUrl":"https://doi.org/10.1163/23524588-00001059","url":null,"abstract":"\u0000This study aimed to evaluate how different food wastes used as growth substrates affected the lipid composition of Hermetia illucens larvae; the relationship among substrates, larvae, and frass fatty acid (FA) composition was studied. Six thousand five-day-old Hermetia illucens larvae were allotted to one of four different substrates named CTRL (a control substrate made of broiler feed), V50 + B50 (vegetable and butchery wastes, 1:1 ratio), V75 + B25 (vegetable and butchery wastes, 3:1 ratio), and V100 (entirely composed of vegetables). Lipids were extracted from substrates, larvae, and frass separately and the FAs were quantified. Saturated FAs (SFAs) prevailed in all the groups. The CTRL larvae had the highest SFA (64.3%), while the V100 and V50 + B50 larvae had the lowest percentages. The V100 frass recorded a significantly higher SFA value (44.8%) than the others. Lauric acid (C12:0) was high in CTRL (0.2%) and V100 (0.3%) substrates and amount at 7.6 and 9.6% in their correspondent frass, respectively. However, C12:0 content was higher in the CTRL larvae than in the V100 ones (36.7 and 24.5%, respectively), while it had an intermediate value (28.9%) in the V75 + B25 larvae. Finally, the n-3 polyunsaturated FAs (PUFAs) level was high in the V100 substrate and larvae but not in the V100 frass. The relationship between frass FAs and their correspondent amounts in substrates and larvae was significant for C12:0, C18:2n-6, MUFAs, and n-6 PUFAs. There was a positive relationship for C12:0 and MUFAs with both substrates and larvae, while for C18:2n-6 and n-6 PUFAs, the relationship was positive for substrates but negative for larvae. In conclusion, the V100 substrate appeared to be the most suitable treatment for rearing Hermetia illucens because of the positive effects on the fatty acid content of larvae (low SFA and high n-3 PUFA content) and frass (optimal lauric acid levels for fertilizers).","PeriodicalId":48604,"journal":{"name":"Journal of Insects as Food and Feed","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139523298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Can black soldier fly larvae (Hermetia illucens) be reared on waste streams for food and feed? – A safety perspective 能否在废物流中饲养黑翅大实蝇幼虫（Hermetia illucens）作为食物和饲料？- 安全观点

IF 5.1 3区农林科学

Journal of Insects as Food and Feed Pub Date : 2024-01-19 DOI: 10.1163/23524588-20230169

Y. Hoffmans, T. Veldkamp, N. Meijer, G.M.H. Brust, M.G.M. van der Schans, T.W. Prins, K. van Rozen, H. Elissen, P. van Wikselaar, R. Y. van der Weide, H. van der Fels-Klerx, E. F. Hoek-van den Hil

{"title":"Can black soldier fly larvae (Hermetia illucens) be reared on waste streams for food and feed? – A safety perspective","authors":"Y. Hoffmans, T. Veldkamp, N. Meijer, G.M.H. Brust, M.G.M. van der Schans, T.W. Prins, K. van Rozen, H. Elissen, P. van Wikselaar, R. Y. van der Weide, H. van der Fels-Klerx, E. F. Hoek-van den Hil","doi":"10.1163/23524588-20230169","DOIUrl":"https://doi.org/10.1163/23524588-20230169","url":null,"abstract":"\u0000The use of insects as feed and food can be part of the solution towards a circular economy, in case the safety of insect products is assured. Black soldier fly larvae (BSFL, Hermetia illucens) can be reared on different waste streams. However, before BSFL can be legally reared on these streams, the safety of BSFL for feed and food should be assessed thoroughly. This study aimed to investigate several food safety aspects of BSFL grown on waste streams. Therefore, BSFL were reared for 7 days on substrate mixtures of waste streams with similar protein and moisture content. These waste streams included fast food waste (FF), mushroom stem (MS), pig manure solids (PS), poultry meal (PM) and slaughter waste (SW). The substrates, BSFL and the frass were analysed for the presence of metals and veterinary drugs. The substrates and BSFL were also analysed for presence of DNA of ruminant, pig and chicken. Some of the metals accumulated in BSFL, although the concentrations in BSFL (as these would be manufactured as feed) were below maximum limits for feed. Only traces of some of the analysed veterinary drugs were found in the BFSL and no accumulation thereof was observed. DNA of ruminant and pig was traced back in BSFL samples, however, chicken was not. A good understanding of the presence of food safety hazards and possible variance thereof in potential substrates, such as waste streams, and their possible residues in insects is necessary for implementation of this circular way of insect feeding in the food chain.","PeriodicalId":48604,"journal":{"name":"Journal of Insects as Food and Feed","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139612088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Basil (Ocimum basilicum) growth and biochemical attributes are influenced by various black soldier fly (Hermetia illucens) larvae frass types 罗勒（Ocimum basilicum）的生长和生化属性受各种黑刺蝇幼虫虫体类型的影响

IF 5.1 3区农林科学

Journal of Insects as Food and Feed Pub Date : 2024-01-18 DOI: 10.1163/23524588-00001061

N. Romano, G.S.J. Pande, S.N. Datta, A. K. Sinha, S. Islam

{"title":"Basil (Ocimum basilicum) growth and biochemical attributes are influenced by various black soldier fly (Hermetia illucens) larvae frass types","authors":"N. Romano, G.S.J. Pande, S.N. Datta, A. K. Sinha, S. Islam","doi":"10.1163/23524588-00001061","DOIUrl":"https://doi.org/10.1163/23524588-00001061","url":null,"abstract":"\u0000A major by-product of black solider fly larvae (BSFL) farming is the leftover mineral-rich ‘frass’, which shows promise as a soil amendment. The composition of BSFL frass, and potentially their efficacy, is influenced by the initial substrate. In this study, expired fish diets (EFD) or fruits/vegetables (FV) were used to produce BSFL frass, which were separately used as a soil amendment for basil versus basil that received weekly applications of a synthetic fertilizer (SF) (nitrogen, phosphorus, and potassium; 18-18-21). After five weeks, measurements of the sugar content, total phenol/antioxidant capacity, and mineral composition were determined. Basil heights became significantly taller with more biomass in SF (283 g) compared to the EFD frass treatment (115 g) but not the FV frass treatment (186 g). Compared with SF, basil leaves were significantly lower in manganese, calcium, zinc and iron in both frass treatments, but magnesium was significantly higher in the frass treatments. While soluble and insoluble sugar were significantly lower in both frass treatments (total of 26 and 13 mg/g in EFD and FV, respectively) compared to SF (43 mg/g), an opposite trend occurred with total phenol/antioxidant capacity. Results indicate deficiencies of some nutrients and more frequent applications of the BSFL frass might be necessary to overcome reduced production. Overall, FV frass enhanced basil production compared to EFD frass indicating that frass type influences plant growth.","PeriodicalId":48604,"journal":{"name":"Journal of Insects as Food and Feed","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139614249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of mealworm hydrolysates on improving immune function and gut microbiota in cyclophosphamide-induced mice 黄粉虫水解物对改善环磷酰胺诱导小鼠免疫功能和肠道微生物群的影响

IF 5.1 3区农林科学

Journal of Insects as Food and Feed Pub Date : 2024-01-17 DOI: 10.1163/23524588-20230175

S.-M. Kim, Y.-S. Lee, V. Jaiswal, J.-Y. Kim, Eun-Jung Park, H.-J. Lee

{"title":"Effects of mealworm hydrolysates on improving immune function and gut microbiota in cyclophosphamide-induced mice","authors":"S.-M. Kim, Y.-S. Lee, V. Jaiswal, J.-Y. Kim, Eun-Jung Park, H.-J. Lee","doi":"10.1163/23524588-20230175","DOIUrl":"https://doi.org/10.1163/23524588-20230175","url":null,"abstract":"\u0000The immune system plays a crucial role in protecting the host from infections and other diseases. Dysregulation of immune function is a risk factor for various noninfectious diseases, including obesity, cardiovascular disease, diabetes, and cancer. Tenebrio molitor larvae, also known as mealworms, are edible insects that are a protein-rich food source. This study investigated the effects of mealworm hydrolysate (MH) on immune enhancement and gut microbiota changes in a cyclophosphamide-induced immunosuppression mouse model. The findings revealed that MH administration increased natural killer (NK) cell activity and cytokine levels. The MH treatment group demonstrated significantly elevated serum cytokine levels and splenic cytokine mRNA expression compared to the model group. MH also increased the phosphorylation of nuclear factor-kappa B (NF-κB) and NF-κB inhibitor alpha (IκBα). Additionally, MH treatment altered the composition of the gut microbiota, increasing the relative abundance of Akkermansia and Corynebacterium, which are linked to immune function. Collectively, this study shows that MH improves immune-related markers and modulates the gut microbiota, implying that it could be used as a functional food for immune enhancement. \u0000\u0000\u0000 \u0000\u0000","PeriodicalId":48604,"journal":{"name":"Journal of Insects as Food and Feed","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139617008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nutritive value of cereal grains, protein meals and by-products for yellow mealworms 谷物、蛋白粉和副产品对黄粉虫的营养价值

IF 5.1 3区农林科学

Journal of Insects as Food and Feed Pub Date : 2024-01-16 DOI: 10.1163/23524588-00001022

J. J. Pascual, B. Fasce, A. Martı́nez-de-Pablo, C. Andrade, M.C. López, M. Cambra-López

{"title":"Nutritive value of cereal grains, protein meals and by-products for yellow mealworms","authors":"J. J. Pascual, B. Fasce, A. Martı́nez-de-Pablo, C. Andrade, M.C. López, M. Cambra-López","doi":"10.1163/23524588-00001022","DOIUrl":"https://doi.org/10.1163/23524588-00001022","url":null,"abstract":"\u0000Nutritive value of raw materials is needed to formulate diets that fulfil the requirements of the yellow mealworms. The objective of this study was to determine the nutritional value of six raw materials commonly used in yellow mealworm feeding (barley grain, corn grain, hominy feed, wheat bran, corn gluten feed and soybean meal) and to propose prediction equations for the nutritive value of raw materials from their chemical composition. From Monday to Friday 5 g of raw material and 20 g of gel (source of water and micronutrients) were daily supplied. After two days of fasting, frass was collected. This procedure was repeated for 6 weeks. During this period, the initial and final weight of the larvae, the consumption of the raw material offered, and the production of faeces were controlled. Raw materials and faeces were analysed for dry matter (DM), ashes, crude protein (CP), ether extract, acid detergent fibre (ADF) and gross energy (GE), from which their coefficients of digestibility were determined. The digestible energy (DE) content of the raw materials was 13.8, 14.2, 11.6, 8.7, 6.9, 11.3 MJ DE/kg DM and the digestible protein (DP) was 61, 52, 48, 74, 70 and 242 g DP/kg DM for the barley grain, corn grain, hominy feed, wheat bran, corn gluten feed and soybean meal, respectively. DE content of raw materials was closely correlated with their content on ashes, starch and ADF (−0.83, +0.69 and −0.65, respectively; ), while DP content was mainly correlated with their content on CP, starch and ether extract (+0.95, +0.60 and −0.69, respectively; ). It can be concluded that mealworms have a good ability to utilize cereal starch, wheat bran diets should be complemented with digestible carbohydrates and protein sources, and soybean meal may be a good source of vegetable protein for mealworms.","PeriodicalId":48604,"journal":{"name":"Journal of Insects as Food and Feed","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139528476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of antibacterial activities of hemolymph and methanol extracts from black soldier fly (Hermetia illucens) 黑背潜蝇（Hermetia illucens）血淋巴和甲醇提取物的抗菌活性评价

IF 5.1 3区农林科学

Journal of Insects as Food and Feed Pub Date : 2024-01-11 DOI: 10.1163/23524588-20230048

Y.H. Luo, B.P. Ye, S.Q. Gao, G.J. Tang, D.P. Li, Y.H. Huang, Z.Z. Huang, X. Yang, A.M. Naman, Y.X. Qian, Z. Tan, X.Y. Zeng, B. Wang

{"title":"Evaluation of antibacterial activities of hemolymph and methanol extracts from black soldier fly (Hermetia illucens)","authors":"Y.H. Luo, B.P. Ye, S.Q. Gao, G.J. Tang, D.P. Li, Y.H. Huang, Z.Z. Huang, X. Yang, A.M. Naman, Y.X. Qian, Z. Tan, X.Y. Zeng, B. Wang","doi":"10.1163/23524588-20230048","DOIUrl":"https://doi.org/10.1163/23524588-20230048","url":null,"abstract":"\u0000Black soldier fly (BSF) has been recently recognized as a promising source of antibacterial substances such as antimicrobial peptides (AMPs) and medium-chain fatty acids (MCFAs). This study aimed to isolate and identify the antibacterial activities of hemolymph and methanol extracts from BSF. The BSF larvae hemolymph increased the growth of bacteria which is likely due to its nutritional effects. However, the bacteria (Listeria monocytogenes, Staphylococcus aureus, Salmonella typhimurium, and Escherichia coli) treated with the hemolymph from bacteria inoculated BSF larvae showed slower growth when compared with the bacteria treated with the unchallenged hemolymph, and such a difference was dramatically eliminated by proteinase K and proteins/peptides between 3-10 kDa were the most active substances that affected the growth of bacteria. However, the antibacterial activity of BSF hemolymph was not strong enough to develop a visible inhibition zone in the Zone of Inhibition test against any of the four bacteria strains above. Methanol extracts from III instar BSF larvae, V instar BSF larvae, and BSF prepupae showed strong antibacterial activities in the Zone of Inhibition test against the four bacteria strains, and it is resistant to proteinase K treatment. The antibacterial activity and the percentage of fatty acids in the methanol extracts increased with age. Heating at 130 °C for 60 min significantly improved the antibacterial ability of methanol extracts against Staphylococcus aureus without changing its fatty acid profile. In conclusion, bacteria-inoculated BSF larvae develop AMPs in hemolymph which exhibit weak antibacterial activities. The methanol extracts of BSF can be produced in large quantities using larvae and have strong antibacterial activity which is attributed to lauric acid and some unknown compounds.","PeriodicalId":48604,"journal":{"name":"Journal of Insects as Food and Feed","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139533439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heteropterans: a treasure trove of therapeutic proteins 异翅类：治疗蛋白质的宝库

IF 5.1 3区农林科学

Journal of Insects as Food and Feed Pub Date : 2024-01-10 DOI: 10.1163/23524588-00001062

M.R. Devi, A.S. Koijam, A. Brockmann, Y. Rajashekar

引用次数: 0

The more, the better? Effect of density on the growth of the lesser mealworm, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) 越多越好？密度对小黄粉虫（Alphitobius diaperinus (Panzer) ）（鞘翅目：黄粉虫科）生长的影响

IF 5.1 3区农林科学

Journal of Insects as Food and Feed Pub Date : 2023-12-29 DOI: 10.1163/23524588-00001034

G. Baliota, C. Rumbos, C. G. Athanassiou

{"title":"The more, the better? Effect of density on the growth of the lesser mealworm, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)","authors":"G. Baliota, C. Rumbos, C. G. Athanassiou","doi":"10.1163/23524588-00001034","DOIUrl":"https://doi.org/10.1163/23524588-00001034","url":null,"abstract":"Understanding the concept of larval density scaling in mealworm farming enables us to analyze and compare the growth patterns of these species and their overall productivity in terms of produced insect biomass. This paper explores the impact of larval density on the development of the lesser mealworm, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae). Seven different larval densities were examined in laboratory trials, ranging from 7.5 to 53 larvae per cm2, while the volumetric density per vial was consistent through the experiment at 10 larvae per cm3. The results showed a significant effect of larval density, restricted though to specific developmental traits of A. diaperinus larvae. Based on the results, the highest overall yield and biomass production was achieved at 53 larvae per cm2, however, this density resulted in prolonged development time and decreased larval growth and feed consumption metrics. It is suggested that a larval density of 30.3 larvae per cm2 could offer a favorable balance for achieving optimal biomass efficiency in terms of the time required for harvest, growth performance, feed utilization and economic feasibility. Nevertheless, to the best of our knowledge, this is the first work on the effect of larval density on the performance of A. diaperinus larvae and aims to contribute to the increase of the profitability of A. diaperinus mass-rearing production systems.","PeriodicalId":48604,"journal":{"name":"Journal of Insects as Food and Feed","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139143209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploiting fall foliage by-products to optimize Tenebrio molitor nutraceutical value 利用秋叶副产品优化褐藻营养价值

IF 5.1 3区农林科学

Journal of Insects as Food and Feed Pub Date : 2023-12-21 DOI: 10.1163/23524588-20230101

A. Brai, M.P. Provenzani, C. Pasqualini, F. Poggialini, C. Vagaggini, F. Tarchi, R. Frosinini, V. Francardi, S. Simoni, E. Dreassi

{"title":"Exploiting fall foliage by-products to optimize Tenebrio molitor nutraceutical value","authors":"A. Brai, M.P. Provenzani, C. Pasqualini, F. Poggialini, C. Vagaggini, F. Tarchi, R. Frosinini, V. Francardi, S. Simoni, E. Dreassi","doi":"10.1163/23524588-20230101","DOIUrl":"https://doi.org/10.1163/23524588-20230101","url":null,"abstract":"Tenebrio molitor larvae (TML) are a novel smart food recently declared safe by the European Food Safety Authority (EFSA). These edible insects are a complete source of digestible proteins, and their farming requires limited space and resources. With the purpose to increase the sustainability of their farming, different nursery wastes produced during fall foliage were used as feed supplements. Punica granatum (PL), Castanea sativa (CSL) and Robinia pseudoacacia fallen leaves (AL), and the leaves of two plants used in traditional medicine, Laurus nobilis (LL) and Mentha arvensis leaves (MAL), which are rich in polyphenols, flavonoids and proanthocyanidins were added to the TML standard diet as sources of antioxidant compounds. The fortified diets were administered to TML for their entire life cycle, and mean weight and survival rate analysed every 15 days, without observing a negative impact. After 45 days, when pupation exceeded 50%, the experiment was interrupted. TML accumulated antioxidant compounds from leaves, important to prevent inflammation and oxidative stress. Accordingly, we observed an increment of the antioxidant and radical scavenging activities respect to the TML fed with the standard diet (STDSL). Differently from STDSL, total fat content remained constant during development, while the quality of fat ameliorated, with a beneficial reduction of SFAs and an improvement of nutritional indexes associated with cardiovascular disease prevention. In addition, we observed an important reduction in n6/n3 ratio and the accumulation of linolenic acid. Taken together, our results strongly support the use of nursery wastes to fortify TML and improve their nutraceutical value.","PeriodicalId":48604,"journal":{"name":"Journal of Insects as Food and Feed","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139167728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A common edible insect (Antheraea assamensis) protein hydrolysate regulates LPS-induced oxidative stress and inflammation by modulating the TLR4/NF- κ β Signaling Pathway 一种常见的可食用昆虫蛋白水解物通过调节 TLR4/NF- κ β 信号通路来调节 LPS 诱导的氧化应激和炎症反应

IF 5.1 3区农林科学

Journal of Insects as Food and Feed Pub Date : 2023-12-20 DOI: 10.1163/23524588-00001012

R.K. Sahu, S. Borthakur, M. Saikia, S. Sarkar, R.S. Ahmed, D. Dasgupta, R. Dhakal, S. Mech, P. Manna, P. Dutta, J. Kalita

{"title":"A common edible insect (Antheraea assamensis) protein hydrolysate regulates LPS-induced oxidative stress and inflammation by modulating the TLR4/NF- κ β Signaling Pathway","authors":"R.K. Sahu, S. Borthakur, M. Saikia, S. Sarkar, R.S. Ahmed, D. Dasgupta, R. Dhakal, S. Mech, P. Manna, P. Dutta, J. Kalita","doi":"10.1163/23524588-00001012","DOIUrl":"https://doi.org/10.1163/23524588-00001012","url":null,"abstract":"Dietary intake of antioxidant and anti-inflammatory-rich foods is a growing approach for fighting inflammation and its associated disorders. Edible insects are gaining popularity as a food source; however, their therapeutic properties, anti-inflammatory activity and their mechanism of action remain largely unexplored. Herein, we evaluate the effectiveness of Antheraea assamensis pupae hydrolysates against oxidative stress and inflammation. The proteins isolated from the pupae were digested with pepsin or pancreatin, or pepsin + pancreatin and passed through a 30 kDa molecular cut-off membrane. The low molecular weight protein hydrolysates were characterised using RP-HPLC, HRMS, XRD, and FTIR-ATR. Hydrolysate from pepsin + pancreatin digestion showed significantly higher DPPH (77.13±2.57%), superoxide (72.08±1.15%) and hydroxyl (56.32±0.90%) radical scavenging activity in a cell-free system. Further, it reduced intercellular ROS production and suppressed the lipopolysaccharide-induced protein expression of TLR4, p-IKKβ, p-NF-, IL-1β, and COX2 in RAW 264.7 macrophages. Furthermore, it inhibited the secretion of pro-inflammatory mediators, including IL-6, IL-1β, and MIP-2. These findings suggest that A. assamensis pupae hydrolysates can be a potential source of bioactive peptides for managing oxidative stress and inflammation.","PeriodicalId":48604,"journal":{"name":"Journal of Insects as Food and Feed","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139169437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0