Ability of black soldier fly larvae to bioaccumulate tocopherols from different substrates and measurement of larval tocopherol bioavailability in vitro

IF 4.7 3区农林科学 Q1 ENTOMOLOGY

Journal of Insects as Food and Feed Pub Date : 2023-11-14 DOI:10.1163/23524588-20230183b

L. Morand-Laffargue, D. Vairo, C. Halimi, E. Chiarello, B. Creton, D. Sabatier, P. Borel

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引用次数: 0

Abstract

Edible insects are an emerging approach to provide sustainable proteins in feed. Black soldier fly larvae (BSFL) can also bioaccumulate micronutrients from various substrates. The purpose of this study was to assess whether BSFL can bioaccumulate significant concentrations of bioavailable α and ɣ-tocopherol (TOC) from vitamin E (VE) rich substrates. BSFL were reared on VE rich substrates, e.g. wheat germ oil, bran, etc. α and γ-tocopherol were quantified in larvae and substrates by HPLC. VE bioaccessibility was estimated using an in vitro model of digestion. Uptake efficiency of micellarized VE by intestinal cell was estimated using Caco-2 cells. BSFL were at least as rich in α-TOC, but not ɣ-TOC, as their rearing substrates. VE bioaccessibility was almost always significantly lower in BSFL than in corresponding substrates. Conversely, VE uptake efficiency was either not significantly different or significantly higher in BSFLs than in substrates. Thus, VE enrichment of BSFL from VE rich substrates, in particular co-products such as brans and oil cakes, could be an innovative way to recycle VE and to provide significant amounts of sustainable VE in farm animal feed.

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黑翅大实蝇幼虫从不同底物中生物累积生育酚的能力以及幼虫生育酚生物利用率的体外测定

食用昆虫是在饲料中提供可持续蛋白质的一种新兴方法。黑甲蝇幼虫（BSFL）也能从各种基质中生物累积微量营养素。本研究的目的是评估 BSFL 是否能从富含维生素 E (VE) 的基质中生物累积大量生物可利用的 α 和ɣ-生育酚 (TOC)。用富含 VE 的基质（如小麦胚芽油、麸皮等）饲养 BSFL，通过 HPLC 对幼虫和基质中的α和γ-生育酚进行定量。利用体外消化模型估算 VE 的生物可接受性。使用 Caco-2 细胞估算了肠细胞对胶束化 VE 的吸收效率。BSFL 至少与饲养底物一样富含 α-TOC，而不是 ɣ-TOC 。在 BSFL 中，VE 的生物可及性几乎总是明显低于相应的基质。相反，BSFL 对 VE 的吸收效率要么没有明显差异，要么明显高于底物。因此，从富含 VE 的基质（特别是诸如麸皮和油饼等副产品）中富集 BSFL 的 VE，可能是一种回收 VE 并在农场动物饲料中提供大量可持续 VE 的创新方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Insects as Food and Feed Agricultural and Biological Sciences-Insect Science

CiteScore

7.00

自引率

17.60%

发文量

133

期刊介绍： The Journal of Insects as Food and Feed covers edible insects from harvesting in the wild through to industrial scale production. It publishes contributions to understanding the ecology and biology of edible insects and the factors that determine their abundance, the importance of food insects in people’s livelihoods, the value of ethno-entomological knowledge, and the role of technology transfer to assist people to utilise traditional knowledge to improve the value of insect foods in their lives. The journal aims to cover the whole chain of insect collecting or rearing to marketing edible insect products, including the development of sustainable technology, such as automation processes at affordable costs, detection, identification and mitigating of microbial contaminants, development of protocols for quality control, processing methodologies and how they affect digestibility and nutritional composition of insects, and the potential of insects to transform low value organic wastes into high protein products. At the end of the edible insect food or feed chain, marketing issues, consumer acceptance, regulation and legislation pose new research challenges. Food safety and legislation are intimately related. Consumer attitude is strongly dependent on the perceived safety. Microbial safety, toxicity due to chemical contaminants, and allergies are important issues in safety of insects as food and feed. Innovative contributions that address the multitude of aspects relevant for the utilisation of insects in increasing food and feed quality, safety and security are welcomed.