Journal of Oral Biosciences最新文献

{"title":"Assessment of biofilm formation on ceramic, metal, and plastic brackets in orthodontic materials by new method using renG-expressing Streptococcus mutans","authors":"Hiroyuki Kato ,&nbsp;Hiroko Yoshida ,&nbsp;Masanori Saito ,&nbsp;Tomomi Hashizume-Takizawa ,&nbsp;Shinichi Negishi ,&nbsp;Hidenobu Senpuku","doi":"10.1016/j.job.2024.100594","DOIUrl":"10.1016/j.job.2024.100594","url":null,"abstract":"<div><h3>Objective</h3><div>Oral biofilm has a high acid-producing capacity, increases the risk of enamel demineralization around brackets, and has been identified as a problem in orthodontic treatment. Here, we assessed the risk of biofilm formation by <em>Streptococcus mutans</em>, which is associated with the development of white spot lesions (WSL) on tooth surfaces, using multibracket devices.</div></div><div><h3>Methods</h3><div>Various types of brackets were used for the biofilm formation assay with <em>S. mutans</em> coated with human saliva, immersed in <em>renG</em>-expressing <em>S. mutans</em> UA159 (strain with the luciferase gene inserted), and incubated overnight at 37 °C under aerobic conditions containing 5% CO₂. The biofilm was washed twice with phosphate-buffered saline (PBS), and 200 μL of luciferin dissolved in PBS was added to each well. The mixture was light shielded and allowed to react for 20 min. Luminescence was measured as the amount of biofilm formed by live cells on the bracket surfaces using an optical emission spectrophotometer.</div></div><div><h3>Results</h3><div>Biofilm formation was greater in plastic brackets than in ceramic and metal brackets in a number-dependent manner. However, biofilm formation was inhibited as the plastic bracket was coated with saliva.</div></div><div><h3>Conclusion</h3><div>For preventive treatments of WSL onset during orthodontic treatment, orthodontists should carefully select and customize brackets based on patient needs, goals, and biomechanical principles. This study developed a new measurement method using <em>renG</em>-expressing <em>S. mutans</em> UA159 to accurately assess active biofilm formation on bracket surfaces.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100594"},"PeriodicalIF":2.6,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142796369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional morphology of myoepithelial cells in the rat salivary glands: A review","authors":"Osamu Amano ,&nbsp;Go Onozawa ,&nbsp;Fuyoko Taira ,&nbsp;Yoshihiro Kawabe ,&nbsp;Kenichi Mizobe ,&nbsp;Miyuki Toda ,&nbsp;Arata Nagasaka ,&nbsp;Yasuhiko Bando ,&nbsp;Koji Sakiyama","doi":"10.1016/j.job.2024.100592","DOIUrl":"10.1016/j.job.2024.100592","url":null,"abstract":"<div><h3>Background</h3><div>The acini, the secretory endpieces of the salivary glands, are composed of serous and/or mucous acinar cells and surrounded by myoepithelial cells. Myoepithelial cells are multipolar, stellate cells with long processes encircling the acini and intercalated ducts. These cells contract to facilitate salivary secretion and transport.</div></div><div><h3>Highlight</h3><div>In rat major salivary gland acini, the morphology of myoepithelial cells varies across glands: parotid glands lack myoepithelial cells, submandibular glands contain \"slender\"-shaped cells, and sublingual glands contain \"macho\"-shaped cells. These morphological variations are thought to depend on the salivary viscosity. Myoepithelial cells in the intercalated ducts exhibit minimal variation across the major salivary glands, with processes oriented parallel to the duct axis. These cells are covered by a thin collagen layer and small fibroblasts, collectively termed \"the peri-intercalated duct sheath.\" Ebner's glands, located beneath the circumvallate and foliate papillae and containing numerous taste buds, develop myoepithelial cells in both the acini and intercalated ducts to facilitate vigorous saliva secretion, enhancing gustatory sensitivity.</div></div><div><h3>Conclusions</h3><div>The morphology of myoepithelial cells is influenced by their functional roles under different anatomical, physiological, and pathological conditions. Increased thickness and branching occur to adapt to salivary viscosity and/or enhance secretion. In the intercalated ducts, myoepithelial cells support salivary transport with the aid of the surrounding collagen layer and fibroblasts in \"the peri-intercalated duct sheath\".</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100592"},"PeriodicalIF":2.6,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142773435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression and localization of adiponectin in myoepithelial cells in sublingual glands of normal and diabetic rats","authors":"Genki Miyake ,&nbsp;Arata Nagasaka ,&nbsp;Yasuhiko Bando ,&nbsp;Koji Sakiyama ,&nbsp;Shoichi Iseki ,&nbsp;Hideaki Sakashita ,&nbsp;Osamu Amano","doi":"10.1016/j.job.2024.100590","DOIUrl":"10.1016/j.job.2024.100590","url":null,"abstract":"<div><h3>Objectives</h3><div>Adiponectin is a hormone produced by adipocytes with anti-atherosclerotic and anti-diabetic properties. We previously discovered that adiponectin is specifically localized in the myoepithelial cells of rat sublingual glands. This study aims to investigate the localization of adiponectin and its receptors, AdipoR1 and AdipoR2, in adult rats, postnatally developing rats, and diabetic model rats.</div></div><div><h3>Methods</h3><div>We examined the localization and expression of adiponectin and its receptors by immunohistochemistry and RT-PCR in the sublingual glands of adult rats and in two diabetic rat models: Streptozotocin (STZ)-treated rats for type 1 diabetes and GK rats for type 2 diabetes.</div></div><div><h3>Results</h3><div>In rat sublingual glands, adiponectin was localized in the cytoplasm of myoepithelial cells, while AdipoR1 and AdipoR2 were localized in the basolateral membrane of mucous acinar cells. In GK rats, there was a significant decrease in the immunoreactivity and mRNA levels of adiponectin, while both AdipoR1 and AdipoR2 expression levels were upregulated. In STZ-treated rats, both adiponectin and its receptors showed reduced expression.</div></div><div><h3>Conclusions</h3><div>Adiponectin acts as a paracrine factor in sublingual myoepithelial cells, influencing salivary secretion through upregulated receptors in acinar cells, particularly in type 2 diabetes. This process is associated with a reduction in myoepithelial adiponectin levels.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100590"},"PeriodicalIF":2.6,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142755513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral microbiome profiles of gingivitis and periodontitis by next-generation sequencing among a group of hospital patients in Korea: A cross-sectional study","authors":"Yeon-Hee Lee ,&nbsp;Hae Jeong Park ,&nbsp;Su-Jin Jeong ,&nbsp;Q-Schick Auh ,&nbsp;Junho Jung ,&nbsp;Gi-Ja Lee ,&nbsp;Seungil Shin ,&nbsp;Ji-Youn Hong","doi":"10.1016/j.job.2024.100591","DOIUrl":"10.1016/j.job.2024.100591","url":null,"abstract":"<div><h3>Objectives</h3><div>The oral microbiome plays an important role in the development and progression of periodontal disease. The purpose of this study was to compare microbial profiles of oral cavities in good health, with gingivitis, and in a state of periodontitis, and to identify novel pathogens involved in periodontal diseases.</div></div><div><h3>Methods</h3><div>One hundred and two participants, including 33 healthy controls, 41 patients with gingivitis, and 28 patients with periodontitis, were included in this cross-sectional study. Salivary oral microbiomes were investigated using 16S rRNA metagenomic sequencing, and the microbial profiles of each group were compared using age- and sex-adjusted general linear models.</div></div><div><h3>Results</h3><div>The abundance of amplicon sequence variants and Chao1 diversity were significantly elevated in the gingivitis and periodontitis groups relative to healthy controls (<em>p</em> = 0.046). Based on linear discriminant analysis (LDA) scores (&gt;2), <em>Tenericutes, Mollicutes, Mycoplasmatales, Mycoplasmataceae, Mycoplasma, Bacteroidaceae,</em> and <em>Phocaeicola</em> were significantly enriched in the gingivitis group, and <em>Synergistetes, Synergistia, Synergistales, Synergistaceae, Fretibacterium, Sinanaerobacter,</em> and <em>Filifactor</em> were enriched in the periodontitis group. The relative abundances of <em>Fretibacterium fastidiosum</em>, <em>Sinanaerobacter chloroacetimidivorans</em>, and <em>Filifactor alocis</em> (<em>q</em> = 0.008, all bacteria) were highest in the periodontitis group and lowest in the control group. The relative abundance of <em>Treponema denticola</em> was significantly elevated in the periodontitis group compared to the other two groups (<em>q</em> = 0.024).</div></div><div><h3>Conclusions</h3><div>Oral microbiomes differed between groups. <em>T. denticola</em>, <em>F. fastidiosum</em>, <em>S. chloroacetimidivorans</em> and <em>F</em>. <em>alocis</em> were significantly more abundant in the periodontitis group than in the control group. Additionally, <em>the abundance of T. denticola</em> and <em>F. fastidiosum</em> in the periodontitis group was significantly different from that in the gingivitis group.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100591"},"PeriodicalIF":2.6,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-fungal effects of slightly acidic electrolyzed water on Candida species","authors":"Chia-Hsin Wu ,&nbsp;Yoshino Kaneyasu ,&nbsp;Kanako Yano ,&nbsp;Hideo Shigeishi ,&nbsp;Honami Kitasaki ,&nbsp;Tomoko Maehara ,&nbsp;Yoshie Niitani ,&nbsp;Toshinobu Takemoto ,&nbsp;Yuichi Mine ,&nbsp;Mi Nguyen-Tra Le ,&nbsp;Miki Kawada-Matsuo ,&nbsp;Hitoshi Komatsuzawa ,&nbsp;Kouji Ohta","doi":"10.1016/j.job.2024.10.005","DOIUrl":"10.1016/j.job.2024.10.005","url":null,"abstract":"<div><h3>Objectives</h3><div>Slightly acidic electrolyzed water (SAEW) is produced by electrolyzing 2–6% diluted hydrochloric acid in a membrane-less chamber, resulting in 5.0–6.5 pH, and can be applied to various foods as a disinfectant. Although SAEW has shown to have bactericidal activity, the details of its anti-fungal effects towards <em>Candida specie<u>s</u></em> remain unknown. Therefore, we examined the fungicidal effects of SAEW on <em>Candida</em> spp. and biofilms on acrylic resins.</div></div><div><h3>Methods</h3><div>The fungicidal effects of SAEW on <em>Candida</em> spp. at different reaction times and total numbers of colonies in culture plates were examined. Subsequently, SAEW was added to <em>Candida</em> spp. biofilms formed on polystyrene plates, and adenosine triphosphate (ATP) in SAEW was measured to examine its fungicidal effects towards <em>Candida</em> spp. biofilms. The fungicidal effect of SAEW on <em>Candida</em> spp. biofilms was determined by counting the number of colonies on the acrylic resin after adding SAEW.</div></div><div><h3>Results</h3><div>SAEW completely killing activity within 1 min with the tested <em>Candida</em> spp. <em>C. albicans</em> and <em>C. glabrata</em> ATP were increased 5 min after adding SAEW compared with the controls, suggesting the removal of biofilm. Of the <em>C. albicans</em> on acrylic resin, &gt;99.9%were killed by SAEW compared to their levels in deionized distilled water (DW) (76.2 × 10²/mL and 43.3 × 10²/mL, respectively). Similarly, 93.1% of C. glabrata were killed by SAEW compared to DW (159.3x102/mL).</div></div><div><h3>Conclusions</h3><div>SAEW may be useful in preventing oral candidiasis as part of oral care.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100573"},"PeriodicalIF":2.6,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142630030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a new antibody drug to treat congenital tooth agenesis","authors":"K. Takahashi ,&nbsp;H. Kiso ,&nbsp;E. Mihara ,&nbsp;J. Takagi ,&nbsp;Y. Tokita ,&nbsp;A. Murashima-Suginami","doi":"10.1016/j.job.2024.10.002","DOIUrl":"10.1016/j.job.2024.10.002","url":null,"abstract":"<div><h3>Background</h3><div>This study aimed to develop a therapeutic agent promoting teeth regeneration from autologous tissues for congenital tooth agenesis, specifically for hypodontia (≤5 missing congenital teeth, 10% prevalence) and oligodontia (≥6 missing congenital teeth, 0.1% prevalence).</div></div><div><h3>Highlight</h3><div>We studied mice genetically deficient in the USAG-1 protein, an antagonist of BMP/Wnt which forms excessive teeth. We identified USAG-1 as a target molecule for increasing the number of teeth. Crossing USAG-1-deficient mice with a congenital tooth agenesis model restored tooth formation. We produced anti-USAG-1 neutralizing antibodies as potential therapeutic agents for the treatment of congenital tooth agenesis. Mice anti-USAG-1 neutralizing antibodies can potentially rescue the developmentally arrested tooth germ programmed to a certain tooth type. A humanized anti-USAG-1 antibody was developed as the final candidate.</div></div><div><h3>Conclusion</h3><div>Targeting USAG-1 shows promise for treating missing congenital tooth. Anti-USAG-1 neutralizing antibodies have been developed and will progress towards clinical trials, which may regenerate missing congenital teeth in conditions, such as hypodontia and oligodontia. The protocol framework for a phase 1 study has been finalized, and preparation for future studies is underway.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 4","pages":"Pages 1-9"},"PeriodicalIF":2.6,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142401587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Herbal medicine Ninjinyoeito inhibits RANKL-induced osteoclast differentiation and bone resorption activity by regulating NF-κB and MAPK pathway","authors":"Kaung Htike ,&nbsp;Kunihiro Yoshida ,&nbsp;Takanori Eguchi ,&nbsp;Katsuki Takebe ,&nbsp;Xueming Li ,&nbsp;Yaxin Qu ,&nbsp;Eiko Sakai ,&nbsp;Takayuki Tsukuba ,&nbsp;Kuniaki Okamoto","doi":"10.1016/j.job.2024.09.007","DOIUrl":"10.1016/j.job.2024.09.007","url":null,"abstract":"<div><h3>Objectives</h3><div>Osteoporosis is a systemic bone metabolism disorder characterized by decreased bone mass and strength. Osteoclasts (OCs) are giant multinucleated cells that regulate bone homeostasis by degrading bone matrix. Excessive OC differentiation and activity can lead to serious bone metabolic disorders including osteoporosis. Current treatments, including antiresorptive drugs, exert considerable adverse effects, including jaw osteonecrosis. Herbal medicines, such as Ninjinyoeito (NYT), may also offer efficacy, but with fewer adverse effects. In this study, we investigated NYT's effects on osteoclastogenesis.</div></div><div><h3>Methods</h3><div>Tartrate-resistant acid phosphatase (TRAP) staining and bone resorption assays were performed to examine NYT's effects on OC differentiation and function. OC-related gene expression at mRNA and protein levels was investigated to confirm NYT's inhibitory action against osteoclastogenesis. We also demonstrated involvement of signaling pathways mediated by IκBα and mitogen-activated protein kinases (MAPK) [extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38] and showed nuclear translocation of nuclear factor of activated T-cell cytoplasmic 1 (NFATc1) and nuclear factor kappa B (NF-κB) p65 during osteoclastogenesis.</div></div><div><h3>Results</h3><div>TRAP staining and bone resorption assays confirmed that NYT significantly inhibited OC differentiation and function. Western blot and RT-PCR results showed that NYT ameliorated osteoclastogenesis by suppressing mRNA and protein level expression of OC-related genes. Moreover, blots and immunocytochemistry (ICC) data clarified that NYT abrogates signaling pathways mediated by IκBα and MAPK (ERK, JNK, p38), and demonstrated nuclear translocation of NFATc1 and NF-κB p65 during OC differentiation.</div></div><div><h3>Conclusions</h3><div>These findings suggest NYT is an alternative therapeutic candidate for treating osteoporosis.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 4","pages":"Pages 49-57"},"PeriodicalIF":2.6,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142376160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potassium nitrate suppresses hyperactivities of Vc neurons of the model with dentin hypersensitivity","authors":"Shiori Sugawara ,&nbsp;Koichi Iwata ,&nbsp;Toshiki Takamizawa ,&nbsp;Masashi Miyazaki ,&nbsp;Masayuki Kobayashi","doi":"10.1016/j.job.2024.09.005","DOIUrl":"10.1016/j.job.2024.09.005","url":null,"abstract":"<div><h3>Objective</h3><div>Potassium nitrate (KNO₃) suppresses nociception induced by dental hypersensitivity (HYS). We aimed to examine the effects of KNO₃ on the neural activity of the trigeminal spinal subnucleus caudalis (Vc) in HYS model rats.</div></div><div><h3>Methods</h3><div>KNO₃ or vehicle was applied to the exposed dentin of HYS rats for 3 days. c-Fos expression and neuronal activity in the Vc after acetone treatment for cold stimulation were examined to evaluate the effects of KNO₃ application on dentin.</div></div><div><h3>Results</h3><div>The number of c-Fos-immunoreactive cells in the Vc was lower in the group that received KNO₃ (KNO₃ group) than in the group that received vehicle (control group). Spike firing of Vc neurons in response to cold stimulation of the dentin was recorded before and after KNO₃ application to the cavity, and the increased neural activity was effectively suppressed by KNO₃ application. Scanning electron microscopy revealed that the dentin tubules were not occluded by deposits in any of the groups.</div></div><div><h3>Conclusions</h3><div>KNO₃-induced suppression of Vc neuronal activity does not involve physical occlusion of the dentin tubules but likely involves suppression of Aδ or C-fiber activities in the tooth pulp, resulting in the suppression of Vc neuronal activities.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 4","pages":"Pages 41-48"},"PeriodicalIF":2.6,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142298231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of rhythmic jaw muscle activities induced by electrical stimulations of the corticobulbar tract during rapid eye movement sleep with those during wakefulness and non-rapid eye movement sleep in freely moving Guinea pigs","authors":"Makoto Higashiyama ,&nbsp;Yuji Masuda ,&nbsp;Ayano Katagiri ,&nbsp;Hiroki Toyoda ,&nbsp;Masaharu Yamada ,&nbsp;Atsushi Yoshida ,&nbsp;Takafumi Kato","doi":"10.1016/j.job.2024.09.004","DOIUrl":"10.1016/j.job.2024.09.004","url":null,"abstract":"<div><h3>Objective</h3><div>Rhythmic jaw muscle activities (RJMAs) occur during rapid eye movement (REM) sleep in humans and animals even though motoneurons are inhibited. The present study compared the characteristics of jaw muscle activities induced by electrical microstimulations of the corticobulbar tract (CT) during REM sleep with those during wakefulness and non-REM sleep.</div></div><div><h3>Methods</h3><div>Eleven guinea pigs were surgically prepared for polygraphic recordings with the implantation of a stimulating electrode. Long- and short-train repetitive electrical microstimulations were applied to the CT under freely moving conditions. The response rate, latency, burst amplitude, and cycle length in the digastric muscle were calculated and cortical and cardiac activities were quantified.</div></div><div><h3>Results</h3><div>Long-train microstimulations induced RJMAs in the digastric muscle followed by masseter muscle activity during wakefulness and non-REM sleep and only induced rhythmic digastric muscle activity during REM sleep. The response rate of RJMAs and the burst amplitude of digastric muscles were significantly lower during REM sleep than during wakefulness and non-REM sleep. However, response latency did not significantly differ between REM sleep and wakefulness. Transient cortical and cardiac changes were associated with RJMAs induced during non-REM sleep, but not during REM sleep. Short-train microstimulations induced a short-latency digastric response, the amplitude of which was significantly lower during REM sleep than during non-REM sleep and wakefulness.</div></div><div><h3>Conclusions</h3><div>These results suggest that the masticatory CPG was activated by electrical CT stimulations independently of the motoneuron inhibitory system during REM sleep.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 4","pages":"Pages 58-66"},"PeriodicalIF":2.6,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142298230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antihypertensive agent losartan promotes tongue squamous cell carcinoma cell proliferation via EGFR/ERK1/2/cyclin D1 signaling axis","authors":"Luo-Yun Wu ,&nbsp;Bor-Chyuan Su ,&nbsp;Hsin-Hsien Yu ,&nbsp;Chih-Cheng Cheng ,&nbsp;Chia-Chi Tsai ,&nbsp;Pei-Ling Hsu ,&nbsp;Chu-Wan Lee","doi":"10.1016/j.job.2024.09.003","DOIUrl":"10.1016/j.job.2024.09.003","url":null,"abstract":"<div><h3>Objective</h3><div>To study the effects of losartan, an angiotensin II receptor blocker, in the SCC4 and SCC25 human tongue squamous cell carcinoma cell lines.</div></div><div><h3>Methods</h3><div>Cell proliferation was measured by MTS/PMS activity and trypan blue exclusion assays. The levels of the cell proliferation marker, cyclin D1, were analyzed by western blotting. Apoptosis was assessed by caspase-3 activation and Annexin V-FITC/propidium iodide double staining. Activation of epidermal growth factor receptor (EGFR) and ERK1/2 was validated by western blotting.</div></div><div><h3>Results</h3><div>Moderate concentrations of losartan enhanced the proliferation of SCC4 and SCC25 cells. However, high losartan concentrations induced apoptosis in SCC4 cells. Losartan activated the EGFR/ERK1/2/cyclin D1 signaling axis, which in turn promoted cell proliferation. Afatinib (EGFR inhibitor) and U0126 (ERK1/2 inhibitor) abolished losartan-induced cell proliferation. In contrast, UC2288 (p21 inhibitor) enhanced it.</div></div><div><h3>Conclusions</h3><div>Losartan exhibited dual effects on tongue squamous cell carcinoma cells. Moderate losartan concentrations facilitated cell proliferation, whereas high concentrations induced cytotoxicity in tongue carcinoma cells.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 4","pages":"Pages 74-80"},"PeriodicalIF":2.6,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142157193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}