Journal of Oral Biosciences最新文献

{"title":"Microbial profiling of bold green tea bottled beverages: A screening experiment","authors":"Manami Imai ,&nbsp;Miho Kawachi ,&nbsp;Anna Wakui ,&nbsp;Misato Miyazawa ,&nbsp;Mirai Sekiguchi ,&nbsp;Yuki Kato ,&nbsp;Haruna Sato ,&nbsp;Yuka Naruse ,&nbsp;Nanami Asano ,&nbsp;Momoko Morohashi ,&nbsp;Hiroto Sano ,&nbsp;Yuki Abiko ,&nbsp;Jumpei Washio ,&nbsp;Kaori Tanaka ,&nbsp;Nobuhiro Takahashi ,&nbsp;Takuichi Sato","doi":"10.1016/j.job.2025.100697","DOIUrl":"10.1016/j.job.2025.100697","url":null,"abstract":"<div><h3>Objectives</h3><div>To explore the storage potential and drinking safety of leftover bottled tea beverages from various manufacturers after directly drinking from the bottle, we conducted a screening experiment on the growth of salivary bacteria in plastic bottles of bold green tea.</div></div><div><h3>Methods</h3><div>Resting whole saliva (n = 14) was collected from each participant (aged 19–25 years). The saliva samples (1.0 mL of each diluted saliva sample [5.0 × 10⁵ CFU/mL]), were inoculated into plastic bottles containing 280 mL of green tea, which included six types of bold green tea beverages. The bottles were stored at 37 °C for 24 h; subsequently, 1.0 mL of each sample was inoculated onto a blood agar plate and incubated anaerobically at 37 °C. Genomic DNA was extracted from the resulting individual colonies and the bacterial species were identified by 16S rDNA sequencing.</div></div><div><h3>Results</h3><div>More than 60 % of the samples of six types of bold green tea beverages, Bold Oi Ocha®, Suntory Bold Green Tea Iyemon®, Bold Oi Ocha Premium Strong®, Healthya®, Bold Ayataka®, and Catechin Green Tea® showed low bacterial levels (&lt;10³ CFU/mL) after 1 day of storage. However, in some cases, former members of the genus <em>Lactobacillus</em>, such as <em>Limosilactobacillus</em> and <em>Lactiplantibacillus</em> spp., were specifically detected as the predominant bacteria (37.6–100 %), although these bacteria usually account for the minority among the oral microbiome.</div></div><div><h3>Conclusions</h3><div>Although the antibacterial effects of catechins may have affected the total bacterial counts and compositions, no clear correlation was observed between total tea catechin concentrations and total bacterial growth inhibitory effects.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 4","pages":"Article 100697"},"PeriodicalIF":2.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Senescence in palatal rugae development","authors":"Finsa Tisna Sari ,&nbsp;Maiko Kawasaki ,&nbsp;Katsushige Kawasaki ,&nbsp;Vanessa Utama ,&nbsp;Alex Kesuma ,&nbsp;Makoto Fukushima ,&nbsp;Takehisa Kudo ,&nbsp;Akira Fujita ,&nbsp;Kaya Ichikawa ,&nbsp;Yurie Sato-Yamada ,&nbsp;Ippei Shimizu ,&nbsp;Tohru Minamino ,&nbsp;Jun Nihara ,&nbsp;Atsushi Ohazama","doi":"10.1016/j.job.2025.100688","DOIUrl":"10.1016/j.job.2025.100688","url":null,"abstract":"<div><h3>Objectives</h3><div>Periodic patterning is a common feature observed in many organs and is believed to share common molecular mechanisms during development. Palatal rugae, periodically patterned ridges located on the mammalian hard palate, provide an excellent experimental model for investigating the mechanisms of periodic patterning.</div></div><div><h3>Methods</h3><div>We investigated senescence during palatal rugae development in wild-type mice.</div></div><div><h3>Results</h3><div>Senescence-associated β-galactosidase (SA-β-gal) activity was observed during palatal rugae development in wild-type mice. During embryonic days (E) 12–14, SA-β-gal activity was detected in the placodes of rugae 1–4 and 8. IL-6 and p21 expression were present in the placodes of these rugae at these stages. SA-β-gal signal began to disappear at E15 and was no longer detectable in all rugae at E16. The expression of macrophage markers was observed as SA-β-gal activity disappeared. SA-β-gal activity persisted in mice with epithelial conditional deletion of <em>p21</em>. Furthermore, we successfully reduced SA-β-gal activity using a senolytic drug.</div></div><div><h3>Conclusions</h3><div>Senescence-like cells, activated via a p21-independent pathway, are present during palatal rugae development.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 4","pages":"Article 100688"},"PeriodicalIF":2.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144826412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fibroblast growth factor 2 promotes matrix vesicle-mediated mineralization of human umbilical cord perivascular cells by regulating phosphate metabolism","authors":"Sakurako Asada ,&nbsp;Risako Chiba-Ohkuma ,&nbsp;Takeo Karakida ,&nbsp;Ryuji Yamamoto ,&nbsp;Shun Nonoyama ,&nbsp;Kazuhiro Gomi ,&nbsp;Takatoshi Nagano ,&nbsp;Yasuo Yamakoshi","doi":"10.1016/j.job.2025.100693","DOIUrl":"10.1016/j.job.2025.100693","url":null,"abstract":"<div><h3>Objectives</h3><div>We aimed to investigate the role of fibroblast growth factor 2 (FGF2) in promoting osteogenic differentiation and mineralization of human umbilical cord perivascular cells (HUCPVCs) and to elucidate the underlying molecular mechanisms, particularly the matrix vesicle-mediated mineralization pathway.</div></div><div><h3>Methods</h3><div>HUCPVCs were cultured under osteogenic conditions with or without FGF2 in the presence of activated vitamin D₃, LDN-193189, and transforming growth factor β1. Mineralization was assessed using alkaline phosphatase (ALP) activity assay, Alizarin Red S staining, ALP staining, and calcium quantification. The selective FGF receptor inhibitor LY2874455 and exogenous tissue-non-specific ALP (TNAP) were used to evaluate the effects of FGF2. Transcriptomic analysis and expression profiling of matrix vesicle-mediated mineralization- and myofibroblast-related markers were performed using next-generation sequencing (NGS), quantitative polymerase chain reaction, and immunofluorescence staining. Extracellular pyrophosphate (PPi) was quantified using colorimetric assays. Additionally, the mineralization capacity of HUCPVCs was assessed on titanium and zirconia disks.</div></div><div><h3>Results</h3><div>FGF2 significantly enhanced ALP activity and mineralization, which were abrogated by LY2874455. FGF2 upregulated TNAP and phosphate transporter 1 while suppressing ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) and alpha-smooth muscle actin expression. In HUCPVCs cultured without FGF2, elevated ENPP1 and PPi levels correlated with reduced mineralization, which was rescued by supplementation with TNAP. NGS revealed upregulation of matrix vesicle-mediated mineralization-related genes. Significant mineralization was observed in HUCPVCs cultured with FGF2 on titanium and zirconia disks.</div></div><div><h3>Conclusions</h3><div>FGF2 enhances HUCPVC-mediated mineralization via dual regulation: ALP induction and ENPP1–PPi suppression. These findings support the therapeutic potential of FGF2 in bone tissue engineering and implant surface integration.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 4","pages":"Article 100693"},"PeriodicalIF":2.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144918881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep learning approaches for pathological image classification","authors":"Masayuki Tsuneki","doi":"10.1016/j.job.2025.100696","DOIUrl":"10.1016/j.job.2025.100696","url":null,"abstract":"<div><h3>Background</h3><div>Computer-aided diagnosis using deep learning has emerged as a transformative tool in pathology that enables the automated and consistent interpretation of whole slide images. Among the key tasks in pathological image analysis, classification-based deep learning models have shown promise in distinguishing cancer subtypes and predicting genetic or molecular features. However, challenges remain due to the limited availability of high-quality labeled datasets, particularly for rare cancers, which hinders the widespread applicability of conventional data-driven approaches.</div></div><div><h3>Highlight</h3><div>The methodology for developing classification-based deep learning models involves data preparation, annotation strategies, training techniques, and performance evaluation. Core supervised learning approaches are used alongside convolutional and recurrent neural networks to enhance classification performance. In scenarios in which training datasets are scarce, transfer learning serves as an effective strategy to improve the model training efficiency. In addition, this review introduces emerging techniques such as the use of simulators to generate synthetic data and formula-driven approaches that simulate realistic scenarios, with the aim of overcoming the limitations of conventional training datasets. Visualization tools such as probability heatmaps are critical for model interpretability and validation.</div></div><div><h3>Conclusion</h3><div>Classification-based deep learning models are expected to play an increasing role in precision medicine by expanding capabilities beyond diagnosis to include prognostic prediction and treatment decision making. With innovations in augmented intelligence, deep learning models can comprehensively support clinicians across the diagnostic and therapeutic spectrum, particularly in an era of increasing demand and limited human resources in pathology.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 4","pages":"Article 100696"},"PeriodicalIF":2.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145010297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of Gremlin1 reduces osteoclast activation and alleviates inflammation-induced bone loss by disrupting the NF-κB signaling pathway","authors":"Yuting Wang ,&nbsp;Wenlan Li ,&nbsp;Yifei Tang ,&nbsp;Wenli Mu ,&nbsp;Mingfei Wang ,&nbsp;Wenjing Liu ,&nbsp;Xiaomei Guo ,&nbsp;Tiezhou Hou ,&nbsp;Xiaoyue Guan","doi":"10.1016/j.job.2025.100686","DOIUrl":"10.1016/j.job.2025.100686","url":null,"abstract":"<div><h3>Objectives</h3><div>Gremlin1 plays a crucial role in the development of various inflammatory diseases that cause bone loss, including periodontitis and apical periodontitis (AP). The impact of Gremlin1 on osteoclast activation and its mechanisms in the progression of inflammation-induced bone loss remains unclear. This study aimed to investigate the role of Gremlin1 in the activation of osteoclasts within the inflamed peri-apical region and its possible mechanism.</div></div><div><h3>Methods</h3><div>A local Gremlin1 knock-down AP animal model was established. Micro-computed tomography and hematoxylin and eosin staining were used to assess the volume of bone defects and the extent of inflammatory infiltration. Tartrate-resistant acid phosphatase staining was used to quantify osteoclast numbers and immunohistochemical staining was used to assess the expression of osteoclast differentiation-related proteins in inflamed peri-apical tissues. Subsequently, THP-1 cells with differential expression of Gremlin1 were stimulated to develop into osteoclasts and the activation of osteoclasts and variables associated with osteoclastogenesis were re-evaluated. The activation level of the NF-κB signaling pathway was examined using in vivo and in vitro models.</div></div><div><h3>Results</h3><div>Local administration of adeno-associated virus-silenced Gremlin1 to peri-apical tissues significantly inhibited alveolar bone loss. The number of osteoclasts and the expression of genes associated with osteoclastogenesis were markedly decreased. The in vivo and in vitro experimental findings were consistent and revealed that the silencing of Gremlin1 effectively suppresses activation of the NF-κB signaling pathway. Conversely, over-expression of Gremlin1 may facilitate activation of this pathway.</div></div><div><h3>Conclusion</h3><div>Gremlin1 regulates osteoclasts differentiation through the NF-κB signaling pathway, thereby attenuating the development of inflammation-induced bone loss.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 4","pages":"Article 100686"},"PeriodicalIF":2.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144863230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydroxyapatite-based cavitary varnish derived from eggshells: An in vitro analysis of dentin surface","authors":"Ana Cláudia Dalmolin ,&nbsp;Márcia Thaís Pochapski ,&nbsp;Geovana Stafin ,&nbsp;Carlos Guilherme Murr ,&nbsp;Sandra Regina Masetto Antunes ,&nbsp;Fábio André dos Santos","doi":"10.1016/j.job.2025.100703","DOIUrl":"10.1016/j.job.2025.100703","url":null,"abstract":"<div><h3>Objectives</h3><div>We aimed to evaluate the effectiveness of a cavitary varnish containing an experimental eggshell-derived hydroxyapatite (HA) on dentin surfaces.</div></div><div><h3>Methods</h3><div>One hundred and twenty bovine dentin samples were prepared and randomly assigned to five groups: distilled water (DW); cavitary varnish (CV); Colgate® Sensitive Pro-Relief™ (CS); commercial HA NanoP™ (NP); and cavitary varnish with experimental HA. After treatment, samples were subjected to simulated brushing. Surface roughness, morphology, chemical composition, dentin tubule diameter, and permeability were evaluated. Data were analyzed using two-way analysis of variance with Bonferroni post-hoc test.</div></div><div><h3>Results</h3><div>HA samples without brushing exhibited the highest surface roughness. Scanning electron microscopy revealed complete dentinal tubule occlusion in HA samples, which persisted after brushing, whereas other groups showed partial occlusion or exposed tubules. Chemical analysis demonstrated lower phosphorus levels in HA samples without brushing, with an increase after brushing; NP and HA exhibited the highest phosphorus levels after brushing. DW samples showed the highest intertubular calcium levels, whereas CS samples showed the lowest levels after brushing. DW presented the largest tubule diameters without brushing, while brushing significantly increased the tubule diameters in all groups except DW group. Regarding dentin permeability, HA samples had significantly reduced hydraulic conductance compared with DW samples without brushing, with a performance comparable to commercial products; after brushing, no significant differences were detected among groups.</div></div><div><h3>Conclusions</h3><div>Eggshell-derived HA promoted complete and persistent dentinal tubule occlusion and significantly reduced the permeability, even after brushing, confirming its potential as a sustainable and cost-effective biomaterial for dental applications.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 4","pages":"Article 100703"},"PeriodicalIF":2.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145332387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spermidine enhances Immunoglobulin A secretory capacity in rat salivary glands: An ex vivo study","authors":"Yuta Hosomi ,&nbsp;Yuko Yamamoto ,&nbsp;Ryuta Endo ,&nbsp;Masahiro Sugimoto ,&nbsp;Wakako Sakaguchi ,&nbsp;Shinya Fuchida ,&nbsp;Toshiya Morozumi ,&nbsp;Juri Saruta ,&nbsp;Keiichi Tsukinoki","doi":"10.1016/j.job.2025.100687","DOIUrl":"10.1016/j.job.2025.100687","url":null,"abstract":"<div><h3>Objectives</h3><div>Salivary Immunoglobulin A (IgA) plays a crucial role in mucosal immunity. However, the mechanisms that regulate its production are unclear. Spermidine (SPD), a polyamine involved in various cellular functions, has been implicated in immune modulation. In this study, we investigated the presence of SPD in rat salivary glands and its potential role in enhancing production of IgA.</div></div><div><h3>Methods</h3><div>Male Wistar rats were fed a low-polyamine diet for eight weeks. Submandibular and parotid glands were harvested and metabolomic analysis was used to determine the SPD concentration, reverse transcription quantitative polymerase chain reaction (RT-qPCR) was used to evaluate gene expression related to polyamine metabolism and autophagy, enzyme-linked immunosorbent assay (ELISA) was used to quantify secretion of IgA, and immunohistochemistry was used to localize spermidine synthase (SRM).</div></div><div><h3>Results</h3><div>Metabolomic analysis revealed that the concentration of SPD was significantly higher in the submandibular gland than in the parotid gland. RT-qPCR revealed higher <em>SRM</em> and <em>LC3b</em> mRNA expression, and lower <em>Rubicon</em> expression in the submandibular gland. Ex vivo SPD supplementation significantly increased secretion of IgA from both glands. Immunohistochemistry confirmed strong SRM expression in the acinar and ductal cells of the submandibular gland.</div></div><div><h3>Conclusions</h3><div>These findings suggest that SPD is endogenously synthesized in rat salivary glands, particularly in the submandibular gland, and may enhance the ex vivo production capacity of IgA in salivary gland tissues. These results provide insights into the role of polyamines in mucosal immunity and suggest potential therapeutic applications of SPD in enhancing oral immune defense.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 4","pages":"Article 100687"},"PeriodicalIF":2.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144830178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Localization of high endothelial venules is important for the prognosis of patients with oral squamous cell carcinoma","authors":"Takashi Niiyama ,&nbsp;Aya Matsuda ,&nbsp;Nako Maishi ,&nbsp;Yasuhiro Hida ,&nbsp;Alam Mohammad Towfik ,&nbsp;Saki Shinohara ,&nbsp;Amane Mizuno ,&nbsp;Ren Zixiao ,&nbsp;Lihong Lin ,&nbsp;Wataru Kakuguchi ,&nbsp;Kenichiro Sakata ,&nbsp;Yoichi Ohiro ,&nbsp;Yoshimasa Kitagawa ,&nbsp;Hiroaki Suzuki ,&nbsp;Michihiro Ueda ,&nbsp;Kyoko Hida","doi":"10.1016/j.job.2025.100695","DOIUrl":"10.1016/j.job.2025.100695","url":null,"abstract":"<div><h3>Objectives</h3><div>Surgery, chemotherapy, and radiation therapy are currently used for the treatment of malignant tumors, and immunotherapy has recently been established as the fourth method for treating cancer. Therefore, cancer cells and their surrounding microenvironments have been the focus of attention. High endothelial venules (HEVs) that mediate lymphocyte extravasation into lymphoid organs have been reported in cancerous tissues. However, the role of HEVs remains controversial. In this study, the clinical significance of HEVs in patients with oral squamous cell carcinoma (OSCC) was examined.</div></div><div><h3>Methods</h3><div>Eighty-three patients with OSCC of the tongue who had surgery as initial treatment were included. HEVs and lymphocytes were immunohistochemically stained, and their numbers and localization were evaluated.</div></div><div><h3>Results</h3><div>The prognosis significantly improved in patients with a high number of HEVs. Additionally, localization of HEVs to the tumor margins was associated with a good prognosis. Patients with localized infiltration of CD8-positive cells at the tumor margin had a significantly better prognosis, although no correlation was observed between the number of CD8-positive cells around the HEVs and the prognosis. Cox proportional hazards model revealed that TNM Stage, localization of HEVs, and localization of CD8-positive cells are prognostic factors affecting disease-free survival in patients with OSCC.</div></div><div><h3>Conclusions</h3><div>The localization of HEVs and CD8-positive cells affect the prognosis of patients with OSCC and they are beneficial prognostic factors.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 4","pages":"Article 100695"},"PeriodicalIF":2.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered expression of tenascin C in pericoronal tissues by odontogenic epithelial cells during tooth eruption","authors":"Kanako Suyama ,&nbsp;Shohei Yoshimoto ,&nbsp;Masahide Taguchi ,&nbsp;Satoko Sumi ,&nbsp;Tetsuya Kumagai ,&nbsp;Kayoko Ogata ,&nbsp;Shirabe Kurihara ,&nbsp;Youichi Kuba ,&nbsp;Kyoko Oka","doi":"10.1016/j.job.2025.100698","DOIUrl":"10.1016/j.job.2025.100698","url":null,"abstract":"<div><h3>Objectives</h3><div>We aimed to elucidate the role of odontogenic epithelium in pericoronal tissue during tooth eruption by investigating the morphology of epithelial cells and expression of tenascin C.</div></div><div><h3>Methods</h3><div>Biopsy samples from human patients diagnosed with lower first molar eruption disorders, along with mandibular molar tissues from ICR mice, collected between postnatal day 10 (PN10) and PN15, were examined. Immunohistochemical analyses were performed to evaluate the expression of cytokeratin, tenascin C, E-cadherin, pSmads, and Ki67. <em>In situ</em> hybridization was used to detect expression of tenascin C mRNA.</div></div><div><h3>Results</h3><div>In human lower first molar specimens, a band-like configuration of epithelial islands was observed in the pericoronal connective tissue, with and without expression of tenascin C. In one patient, a high level of tenascin C mRNA was detected in the continuous epithelial layer covering the crown of impacted first molars, and the expression of tenascin C correlated with Ki67, a marker of cell proliferation. During lower molar tooth eruption in mice, odontogenic epithelial cells transformed from a mesh-like to a sheet-like configuration, accompanied by a reduction in the expression of tenascin C protein and mRNA. There was no proliferation or expression of pSmads in the epithelial component.</div></div><div><h3>Conclusions</h3><div>Persistent secretion of tenascin C by odontogenic epithelial cells may promote proliferation of the cells and increase accumulation of tenascin C in the pericoronal tissue. These findings suggest that sustained expression of tenascin C suppresses the progression of tooth eruption.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 4","pages":"Article 100698"},"PeriodicalIF":2.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Piezo1 negatively regulates proliferation, but enhances mineralization in human cementoblasts","authors":"Toshihiro Hasegawa ,&nbsp;Takehito Ouchi ,&nbsp;Ryuya Kurashima ,&nbsp;Maki Kimura ,&nbsp;Seiji Asoda ,&nbsp;Taneaki Nakagawa ,&nbsp;Yoshiyuki Shibukawa","doi":"10.1016/j.job.2025.100705","DOIUrl":"10.1016/j.job.2025.100705","url":null,"abstract":"<div><h3>Objectives</h3><div>Cementoblasts play essential roles in secreting collagenous and non-collagenous matrix proteins and in mineralization to produce cementum. Cementum is a mineralized tissue deposited in layers on the surface of the tooth root, where it is subjected throughout life to mechanical stresses including chewing and occlusal forces. To date, the detailed mechanosensitivity mechanisms regulating cementoblasts remain unclear.</div></div><div><h3>Methods</h3><div>We investigated cellular functions driven by mechanosensitive processes in human cementoblasts (HCEM) by analyzing protein expression using immunofluorescence staining and mechanical stimulation-induced Ca²⁺ signaling by measuring intracellular free Ca²⁺ concentration ([Ca²⁺]_i) using the Ca²⁺ indicator fura-2. We also assessed cell proliferation modulation using colony-forming unit fibroblast (CFU–F) analysis, and mineralization efficacy using Alizarin Red and von Kossa staining.</div></div><div><h3>Results</h3><div>HCEM were immunopositive for cementoblast marker proteins, cementum attachment protein, and cementum protein 1, and the mechanosensitive cation channels Piezo1 and Piezo2. Both direct mechanical stimulation and pharmacological stimulation with the Piezo1 activator Yoda1 elicited transient increases in [Ca²⁺]_i, which were significantly suppressed by Dooku1, a pharmacological Yoda1 inhibitor. In the CFU-F assay, colony formation was significantly enhanced by treatment with pharmacological Piezo1 inhibitors, and by GsMTx4, Dooku1, and Piezo1 gene silencing, but was suppressed by Yoda1. HCEM mineralization efficacy was significantly promoted by Yoda1 but significantly suppressed by GsMTx4, Dooku1, and Piezo1 gene silencing.</div></div><div><h3>Conclusions</h3><div>Piezo1 suppresses colony formation, but activates HCEM-mediated mineralization, suggesting that Piezo1-induced Ca²⁺ signaling plays an important role in cementum mineralization and deposition by cementoblasts.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 4","pages":"Article 100705"},"PeriodicalIF":2.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145416052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}