Anti-fungal effects of slightly acidic electrolyzed water on Candida species.

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences Pub Date : 2024-11-06 DOI:10.1016/j.job.2024.10.005

Chia-Hsin Wu, Yoshino Kaneyasu, Kanako Yano, Hideo Shigeishi, Honami Kitasaki, Tomoko Maehara, Yoshie Niitani, Toshinobu Takemoto, Yuichi Mine, Mi Nguyen-Tra Le, Miki Kawada-Matsuo, Hitoshi Komatsuzawa, Kouji Ohta

{"title":"Anti-fungal effects of slightly acidic electrolyzed water on Candida species.","authors":"Chia-Hsin Wu, Yoshino Kaneyasu, Kanako Yano, Hideo Shigeishi, Honami Kitasaki, Tomoko Maehara, Yoshie Niitani, Toshinobu Takemoto, Yuichi Mine, Mi Nguyen-Tra Le, Miki Kawada-Matsuo, Hitoshi Komatsuzawa, Kouji Ohta","doi":"10.1016/j.job.2024.10.005","DOIUrl":null,"url":null,"abstract":"Objectives: Slightly acidic electrolyzed water (SAEW) is produced by electrolyzing 2-6% diluted hydrochloric acid in a membrane-less chamber, resulting in 5.0-6.5 pH, and can be applied to various foods as a disinfectant. Although SAEW has shown to have bactericidal activity, the details of its anti-fungal effects towards Candida species remain unknown. Therefore, we examined the fungicidal effects of SAEW on Candida spp. and biofilms on acrylic resins.Methods: The fungicidal effects of SAEW on Candida spp. at different reaction times and total numbers of colonies in culture plates were examined. Subsequently, SAEW was added to Candida spp. biofilms formed on polystyrene plates, and adenosine triphosphate (ATP) in SAEW was measured to examine its fungicidal effects towards Candida spp. biofilms. The fungicidal effect of SAEW on Candida spp. biofilms was determined by counting the number of colonies on the acrylic resin after adding SAEW.Results: SAEW completely killing activity within 1 min with the tested Candida spp.. C. albicans and C. glabrata ATP were increased 5 min after adding SAEW compared with the controls, suggesting the removal of biofilm. Of the C. albicans on acrylic resin, > 99.9%were killed by SAEW compared to their levels in deionized distilled water (DW) (76.2x102/mL and 43.3x102/mL, respectively). Similarly, 93.1% of C. glabrata were killed by SAEW compared to DW (159.3x102/mL).Conclusions: SAEW may be useful in preventing oral candidiasis as part of oral care.","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":" ","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Oral Biosciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.job.2024.10.005","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives: Slightly acidic electrolyzed water (SAEW) is produced by electrolyzing 2-6% diluted hydrochloric acid in a membrane-less chamber, resulting in 5.0-6.5 pH, and can be applied to various foods as a disinfectant. Although SAEW has shown to have bactericidal activity, the details of its anti-fungal effects towards Candida species remain unknown. Therefore, we examined the fungicidal effects of SAEW on Candida spp. and biofilms on acrylic resins.

Methods: The fungicidal effects of SAEW on Candida spp. at different reaction times and total numbers of colonies in culture plates were examined. Subsequently, SAEW was added to Candida spp. biofilms formed on polystyrene plates, and adenosine triphosphate (ATP) in SAEW was measured to examine its fungicidal effects towards Candida spp. biofilms. The fungicidal effect of SAEW on Candida spp. biofilms was determined by counting the number of colonies on the acrylic resin after adding SAEW.

Results: SAEW completely killing activity within 1 min with the tested Candida spp.. C. albicans and C. glabrata ATP were increased 5 min after adding SAEW compared with the controls, suggesting the removal of biofilm. Of the C. albicans on acrylic resin, > 99.9%were killed by SAEW compared to their levels in deionized distilled water (DW) (76.2x10²/mL and 43.3x10²/mL, respectively). Similarly, 93.1% of C. glabrata were killed by SAEW compared to DW (159.3x102/mL).

Conclusions: SAEW may be useful in preventing oral candidiasis as part of oral care.

查看原文本刊更多论文

微酸性电解水对念珠菌的抗真菌作用

目的：微酸性电解水（SAEW）是通过在无膜室中电解 2-6% 的稀盐酸而产生的，pH 值为 5.0-6.5，可用作各种食品的消毒剂。虽然 SAEW 具有杀菌活性，但其对念珠菌的抗真菌效果尚不清楚。因此，我们研究了 SAEW 对念珠菌属和丙烯酸树脂上的生物膜的杀菌效果：方法：研究 SAEW 在不同反应时间下对念珠菌属的杀菌效果以及培养皿中的菌落总数。随后，在聚苯乙烯板上形成的念珠菌生物膜中加入 SAEW，测定 SAEW 中的三磷酸腺苷（ATP），以考察其对念珠菌生物膜的杀菌效果。加入 SAEW 后，通过计数丙烯酸树脂上的菌落数量来确定 SAEW 对念珠菌属生物膜的杀菌效果：结果：SAEW 在 1 分钟内完全杀死了被测试的念珠菌属。与对照组相比，加入 SAEW 5 分钟后，白色念珠菌和光滑念珠菌的 ATP 增加，表明生物膜已被清除。与它们在去离子水（DW）中的含量（分别为 76.2x102/mL 和 43.3x102/mL）相比，SAEW 杀死了丙烯酸树脂上 99.9% 以上的白僵菌。同样，与去离子水（159.3x102/mL）相比，SAEW杀死了93.1%的水虱：结论：作为口腔护理的一部分，SAEW 可用于预防口腔念珠菌病。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Oral Biosciences DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

4.40

自引率

12.50%

发文量

审稿时长

37 days