Expression and localization of adiponectin in myoepithelial cells in sublingual glands of normal and diabetic rats

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences Pub Date : 2024-11-28 DOI:10.1016/j.job.2024.100590

Genki Miyake , Arata Nagasaka , Yasuhiko Bando , Koji Sakiyama , Shoichi Iseki , Hideaki Sakashita , Osamu Amano

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Abstract

Objectives

Adiponectin is a hormone produced by adipocytes with anti-atherosclerotic and anti-diabetic properties. We previously discovered that adiponectin is specifically localized in the myoepithelial cells of rat sublingual glands. This study aims to investigate the localization of adiponectin and its receptors, AdipoR1 and AdipoR2, in adult rats, postnatally developing rats, and diabetic model rats.

Methods

We examined the localization and expression of adiponectin and its receptors by immunohistochemistry and RT-PCR in the sublingual glands of adult rats and in two diabetic rat models: Streptozotocin (STZ)-treated rats for type 1 diabetes and GK rats for type 2 diabetes.

Results

In rat sublingual glands, adiponectin was localized in the cytoplasm of myoepithelial cells, while AdipoR1 and AdipoR2 were localized in the basolateral membrane of mucous acinar cells. In GK rats, there was a significant decrease in the immunoreactivity and mRNA levels of adiponectin, while both AdipoR1 and AdipoR2 expression levels were upregulated. In STZ-treated rats, both adiponectin and its receptors showed reduced expression.

Conclusions

Adiponectin acts as a paracrine factor in sublingual myoepithelial cells, influencing salivary secretion through upregulated receptors in acinar cells, particularly in type 2 diabetes. This process is associated with a reduction in myoepithelial adiponectin levels.

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脂联素在正常和糖尿病大鼠舌下腺肌上皮细胞中的表达和定位。

目的：脂联素是一种由脂肪细胞产生的具有抗动脉粥样硬化和抗糖尿病特性的激素。我们先前发现脂联素特异性地定位于大鼠舌下腺的肌上皮细胞。本研究旨在探讨脂联素及其受体AdipoR1和AdipoR2在成年大鼠、出生后发育大鼠和糖尿病模型大鼠中的定位。方法：采用免疫组织化学和RT-PCR方法检测脂联素及其受体在成年大鼠和两种糖尿病大鼠模型中的定位和表达：链脲佐菌素（STZ）治疗的1型糖尿病大鼠和GK治疗的2型糖尿病大鼠。结果：在大鼠舌下腺中，脂联素定位于肌上皮细胞的细胞质中，而AdipoR1和AdipoR2定位于粘液腺泡细胞的基底外膜中。GK大鼠免疫反应性和脂联素mRNA水平显著降低，AdipoR1和AdipoR2表达水平上调。在stz处理的大鼠中，脂联素及其受体的表达均降低。结论：脂联素在舌下肌上皮细胞中作为旁分泌因子，通过腺泡细胞中受体的上调影响唾液分泌，特别是在2型糖尿病中。这一过程与肌上皮脂联素水平的降低有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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