Journal of Oral Biosciences最新文献

{"title":"Impact of enamel matrix proteins combined with collagen membranes on osteoblastic cell cultures","authors":"Vanessa Cordeiro Silva ,&nbsp;Gabrielle Moreira Matos ,&nbsp;Iara Gonçalves de Aquino ,&nbsp;Elizabeth Ferreira Martinez ,&nbsp;Larissa Moreira Spinola de Castro-Raucci ,&nbsp;Andresa Borges Soares ,&nbsp;Vera Cavalcanti de Araújo ,&nbsp;Lucas Novaes Teixeira","doi":"10.1016/j.job.2025.100659","DOIUrl":"10.1016/j.job.2025.100659","url":null,"abstract":"<div><h3>Objective</h3><div>Biodegradable collagen membranes are widely used in guided bone regeneration. This study evaluated the effect of enamel matrix proteins (Emdogain®) combined with two collagen membranes (Bio-Gide® or Collprotect®) on osteoblastic cell cultures.</div></div><div><h3>Methods</h3><div>Human osteoblastic cells were cultured on Bio-Gide® or Collprotect® membranes coated with Emdogain® or left uncoated. The assessed parameters included amelogenin quantification at 1 h, 12 h, 1 day, 3 days, and 7 days; cell morphology at 1 day; cell proliferation at 1, 2, and 3 days; gene expression of <em>COL1A1</em>, <em>IBSP</em>, <em>SPP1</em>, and <em>BGLAP</em> at 1 day; Collagen type I quantification at 3 and 7 days; alkaline phosphatase (ALP) activity at 3 and 7 days; and mineralization at 14 days. Data were analyzed using two-way analysis of variance or the Kruskal-Wallis test.</div></div><div><h3>Results</h3><div>Higher amelogenin levels were detected in Bio-Gide® cultures than in Collprotect® cultures after 3 and 7 days. Cells adhered and spread in all experimental groups. Cell proliferation was higher in Bio-Gide® cultures after 3 days (p &lt; 0.05). Gene expression of <em>COL1A1</em>, <em>IBSP</em>, and <em>SPP1</em> was greater in Bio-Gide® cultures with Emdogain® after 1 day. There was higher collagen type I secretion by cultures grown on collagen membranes coated with Emdogain®, particularly on Bio-Gide® cultures at 7 days. ALP activity was also higher in Bio-Gide® cultures at 7 days (p &lt; 0.05). Greater mineralization was detected in cultures grown on Bio-Gide® with Emdogain® (p &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>Combining collagen membranes, particularly Bio-Gide®, with enamel matrix proteins promotes osteogenesis in vitro, potentially advancing bone tissue regeneration and preservation methods.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100659"},"PeriodicalIF":2.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Saliva composition from birth to adolescence: a systematic review of the literature","authors":"Samira Moradi ,&nbsp;Floris J. Bikker ,&nbsp;Daniela Hesse","doi":"10.1016/j.job.2025.100661","DOIUrl":"10.1016/j.job.2025.100661","url":null,"abstract":"<div><h3>Background</h3><div>This systematic review examined the saliva composition of healthy children from birth to 18 years of age by assessing the salivary flow rate, pH, buffering capacity, and ion levels. This review followed the PRISMA guidelines. A systematic search was performed using PubMed, the Cochrane Library, and Scopus. Information regarding the salivary flow rate, pH, buffering capacity, and ion levels in whole saliva was systematically collected, and a qualitative synthesis was performed. The methodological quality of the studies was assessed using the JBI Critical Appraisal Tools.</div></div><div><h3>Highlight</h3><div>Of the 3,268 retrieved studies, 41 were eligible for inclusion. Unstimulated salivary flow showed an age-related decrease, whereas stimulated salivary flow increased with age. The pH of the saliva remained consistent across different ages, whereas the buffering capacity showed an age-related increase. Salivary sodium, calcium, and potassium levels were lower in the younger children than in the older children. An investigation of salivary ion levels revealed lower average fluoride concentrations in Asian populations than in European populations. Most studies had a low or moderate risk of bias.</div></div><div><h3>Conclusion</h3><div>This systematic review highlights age-dependent differences in salivary flow and composition in healthy children from birth to 18 years of age. The flow rate of unstimulated saliva decreased with age, while the flow rate of stimulated saliva increased. The salivary pH remained stable, whereas the buffering capacity increased with age. Furthermore, salivary sodium, calcium, and potassium levels tend to increase with age. These findings underscore the dynamic nature of salivary composition from childhood to adolescence.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100661"},"PeriodicalIF":2.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive anatomical dissection procedure with special reference to the layer-structured facial muscles and fasciae and mouth floor","authors":"Hisako Takami ,&nbsp;Yuka Kobayashi ,&nbsp;Sanako Makishi-Takano ,&nbsp;Yuji Katsumi ,&nbsp;Noboru Sato ,&nbsp;Hayato Ohshima","doi":"10.1016/j.job.2025.100660","DOIUrl":"10.1016/j.job.2025.100660","url":null,"abstract":"<div><h3>Objectives</h3><div>Conventional head and neck practice procedures fail to address several clinical issues. Anatomical structures were removed from the surface layers in that order, and the dissection was shifted to the deeper layer. This approach makes it difficult for medical and dental students to understand the relationships among various anatomical structures. Furthermore, it is difficult to determine the risk on the mandibular nerves and blood vessels during conventional mandible removal procedures. This study aims to develop an anatomical technique that maintains the skin, superficial facial muscles/fasciae, and mandibles, and to reveal the relationships among the bones, muscles, fasciae, blood vessels, and nerves of the face and floor of the mouth.</div></div><div><h3>Methods</h3><div>A total of 43 human cadavers were examined during gross anatomy courses at Niigata University from 2018 to 2022.</div></div><div><h3>Results</h3><div>Using this dissection method, students were able to understand the relationship between the facial muscles/fasciae, nerves, blood vessels, and muscles without removing the skin. Furthermore, this dissection helps surgeons to understand the risk of nerve and vascular damage during surgery as well as the efficiency of facelift methods.</div></div><div><h3>Conclusions</h3><div>This study provided a clarity on anatomical dissection in the head and neck region and the relationship between anatomical structures from a clinical perspective.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100660"},"PeriodicalIF":2.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of DLX3/SAHH axis on osteogenic differentiation of BMSCs in alveolar bone","authors":"Shichen Sun ,&nbsp;Yuxi Jiang ,&nbsp;Kang Chen ,&nbsp;Xinyi Chen ,&nbsp;Jinyou Chai ,&nbsp;Haipeng Sun","doi":"10.1016/j.job.2025.100658","DOIUrl":"10.1016/j.job.2025.100658","url":null,"abstract":"<div><h3>Objectives</h3><div>Bone marrow mesenchymal stem cells (BMSCs) promote alveolar bone formation and repair. Distal-less homeobox 3 (DLX3) plays a key regulatory role in BMSC osteogenesis. However, the precise pathway by which it mediates osteogenesis in BMSCs remains unclear. In this study, we investigated the potential epigenetic mechanisms underlying DLX3-mediated BMSCs osteogenesis.</div></div><div><h3>Methods</h3><div>BMSCs were isolated from the alveolar bone. DLX3 overexpression and knockdown cell lines were established using lentivirus-mediated gene transfer. Osteogenic differentiation was evaluated using alkaline phosphatase expression, alizarin red staining, real-time quantitative polymerase chain reaction, western blotting, chromatin immunoprecipitation, RNA immunoprecipitation, and S-adenosyl-homocysteine hydrolase (SAHH) activity measurements.</div></div><div><h3>Results</h3><div>DLX3 enhanced the osteogenic differentiation of BMSCs, positively regulated SAHH, and enhancer of zeste homolog 2 (EZH2) activity. In addition, the long non-coding RNA H19 (H19) bound to SAHH in BMSCs. DLX3 regulated the expression of H19, and rescue experiments showed that H19 knockdown increased SAHH activity, thereby promoting osteogenic differentiation in the DLX3-overexpression group.</div></div><div><h3>Conclusions</h3><div>The DLX3/SAHH axis may regulate the activity of EZH2 involved in the osteogenic differentiation of BMSCs.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100658"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suppressive effects of lemon myrtle extract against the colonization and virulence factors of Candida spp.","authors":"Siyuan Liu ,&nbsp;Yi Wang ,&nbsp;Chun Xu","doi":"10.1016/j.job.2025.100657","DOIUrl":"10.1016/j.job.2025.100657","url":null,"abstract":"<div><h3>Objectives</h3><div><em>Candida</em> species (<em>Candida</em> spp.) are among the most common opportunistic pathogens inhabiting the oral cavity and frequently cause infection in immunocompromised individuals. Conventional antibiotic treatments for <em>Candida</em> infections face significant challenges, including the emergence of antimicrobial resistance. This highlights the urgent need for alternative therapeutic strategies, particularly those leveraging natural products.</div></div><div><h3>Methods</h3><div>In this study, we evaluated the inhibitory effects of an aqueous lemon myrtle extract on the colonization and virulence of six <em>Candida</em> spp., including microbial adhesion, biofilm formation, extracellular polysaccharide production, hyphal production, and several invasion-associated virulence factors.</div></div><div><h3>Results</h3><div>The extract significantly reduced <em>Candida</em> adhesion to hard surfaces and inhibited biofilm formation. Additionally, it suppressed the production of insoluble extracellular polysaccharides and various invasion-associated virulence factors, including phospholipase, ergosterol, protease, and hyphal formation.</div></div><div><h3>Conclusions</h3><div>These findings provide a better understanding of the potential role of lemon myrtle extract as a natural therapeutic agent for controlling <em>Candida</em> colonization and mitigating its invasive capabilities. This study provides a foundation for further exploration of lemon myrtle as a promising alternative for the management of <em>Candida</em> infections.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100657"},"PeriodicalIF":2.6,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial signatures in oral submucous fibrosis and oral squamous cell carcinoma: Implications of tobacco and betel quid consumption","authors":"Namrata Jiya ,&nbsp;Avinash Sanap ,&nbsp;Shruti Srivastava ,&nbsp;Supriya Kheur ,&nbsp;Avinash Sharma","doi":"10.1016/j.job.2025.100656","DOIUrl":"10.1016/j.job.2025.100656","url":null,"abstract":"<div><h3>Objectives</h3><div>We compared the oral bacteriome and mycobiome of patients with oral submucosal fibrosis (OSMF) and oral squamous cell carcinoma (OSCC) who consumed tobacco/betel quid with those of healthy individuals.</div></div><div><h3>Methods</h3><div>Targeted amplicon sequencing coupled with bioinformatics analysis was performed to assess the bacterial and fungal communities in the oral cavity.</div></div><div><h3>Results</h3><div>Distinct variations in the microbial communities were observed among the healthy, OSMF, and OSCC samples. Firmicutes was dominant at the phylum level: 21.71 %, 21.73 %, and 24.56 % in the healthy control, OSMF, and OSCC samples, respectively. <em>Lactobacillus</em> (9.6 %, 9.04 %, and 8.9 %) and <em>Streptococcus</em> (7.3 %, 7.55 %, and 6.1 %) showed minimal variation among the healthy, OSMF, and OSCC samples, respectively, at the genus level. <em>Rothia</em> (2.73 %) and <em>Veillonella</em> (1.52 %) were prominent in those with OSMF, and <em>Gemella</em> (2.2 %) and <em>Fusobacterium</em> (1.5 %) were prominent in the OSCC samples. The oral mycobiome was dominated by Ascomycota and Basidiomycota in all samples, with Ascomycota accounting for 18.95 %, 16.05 %, and 15.87 %, and Basidiomycota for 13.55 %, 15.79 %, and 15.96 % in the OSCC, OSMF, and healthy samples, respectively. PICRUSt2 analysis revealed the key metabolic pathways linked to disease and xenobiotic degradation, related to the harmful compounds from tobacco, highlighting the clinical relevance of tobacco and betel quid associated microbial communities as well as the differences between OSCC and OSMF.</div></div><div><h3>Conclusions</h3><div>Microbial signatures associated with OSCC and OSMF could serve as biomarkers for early cancer detection. These findings highlight how tobacco and betel quid consumption promote cancer and alter the oral microbome.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100656"},"PeriodicalIF":2.6,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alendronate inhibits bone-specific blood vessels in the femoral metaphyses of mice","authors":"Tomoka Hasegawa ,&nbsp;Mako Sakakibara ,&nbsp;Xuanyu Liu ,&nbsp;Hirona Yoshino ,&nbsp;Yan Shi ,&nbsp;Jiaxin Cui ,&nbsp;Mai Haraguchi-Kitakamae ,&nbsp;Weisong Li ,&nbsp;Wang Haoyu ,&nbsp;Tomomaya Yamamoto ,&nbsp;Hotaka Ishizu ,&nbsp;Tamaki Sekiguchi ,&nbsp;Tomohiro Shimizu ,&nbsp;Norio Amizuka","doi":"10.1016/j.job.2025.100655","DOIUrl":"10.1016/j.job.2025.100655","url":null,"abstract":"<div><div>To determine whether alendronate affects vascular endothelial cells and regulates the interactions between blood vessels and osteoblasts, we have examined the femoral metaphyses of alendronate-administered mice. Following administration, the bone-specific blood vessels exhibited significantly reduced luminal diameters and rough luminal surfaces with numerous small protrusions and vesicles. Although the osteoclast distribution remained unchanged in alendronate-treated mice, osteoblasts were inactivated in the metaphyseal regions where blood vessels had shrunk. Additionally, the expression of genes such as <em>Ephb4</em>/<em>Efnb2</em>, which mediate vascular endothelial cell–osteoblast interactions, was diminished. Therefore, alendronate may primarily affect bone-specific blood vessels, thus leading to osteoblast inactivation.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100655"},"PeriodicalIF":2.6,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of antigen-induced arthritis and compressive mechanical stress on condylar head of mandible","authors":"Shinnosuke Nogami ,&nbsp;Tomonari Kajita ,&nbsp;Yuta Yanagisawa ,&nbsp;Hikari Suzuki ,&nbsp;Yuri Takeda ,&nbsp;Ko Ito ,&nbsp;Akira Kumasaka ,&nbsp;Christoph Steiner ,&nbsp;Alexander Gaggl ,&nbsp;Masahiro Iikubo ,&nbsp;Hiroyuki Kumamoto ,&nbsp;Kensuke Yamauchi","doi":"10.1016/j.job.2025.100654","DOIUrl":"10.1016/j.job.2025.100654","url":null,"abstract":"<div><h3>Objective</h3><div>Compressive mechanical stress was applied to the temporomandibular joints (TMJ) affected by antigen-induced arthritis to assess the etiological factors of idiopathic condylar resorption.</div></div><div><h3>Methods</h3><div>Following ovariectomy in 27 female rabbits, 12 received ovalbumin (OVA), 12 received phosphate-buffered saline, and three formed the control group. Each treated rabbit underwent an osteotomy, and a custom device was employed for one week postoperatively, with the length increased by 0.25 mm every 12 h to provide compressive mechanical stress to the TMJ. Thereafter, samples were obtained from the treated groups, subjected to histological staining and immunohistochemistry, and examined using micro-computed tomography.</div></div><div><h3>Results</h3><div>At each examination, the OVA group showed a greater area and depth of bone resorption, with bone resorption continuing for three weeks following distraction. Additionally, subcondylar bone resorption was noted significantly earlier and had a greater prevalence in the OVA group and greater numbers of tartrate-resistant acid phosphatase-positive cells. Immunostaining for metalloproteinase (MMP)-3 and MMP-13 of the anterior condylar head in the OVA group after two and three weeks revealed high levels of both proteins from the surface to the deep cartilage layer.</div></div><div><h3>Conclusion</h3><div>Therefore, coexisting TMJ pathology factors, such as antigen-induced arthritis, promote a significantly greater amount of condylar head anterior surface bone resorption.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100654"},"PeriodicalIF":2.6,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyclic compression loading alters osteoarthritis-related gene expression in three-dimensionally cultured human articular chondrocytes via a different mechanism than interleukin-1β induction","authors":"Minami Hikida ,&nbsp;Takashi Kanamoto ,&nbsp;Yoshihito Tachi ,&nbsp;Kosuke Ebina ,&nbsp;Masahiro Nakajima ,&nbsp;Ken Nakata","doi":"10.1016/j.job.2025.100653","DOIUrl":"10.1016/j.job.2025.100653","url":null,"abstract":"<div><h3>Objectives</h3><div>Mechanical and inflammatory stimuli are key factors in the pathophysiology of osteoarthritis (OA). However, the effects of mechanical stimulation on joint tissues and cells at the molecular level and the mechanisms of interaction after stimulation with inflammatory cytokines remains uninvestigated.</div></div><div><h3>Methods</h3><div>Three-dimensional cyclic compression loading (CCL) was applied to human articular chondrocytes, and the expression of OA-related genes was analyzed using reverse transcription quantitative real-time polymerase chain reaction. Additionally, the effects of CCL after the chondrocytes were stimulated with interleukin (IL)-1β were evaluated. A DNA microarray assay was used to compare changes in gene expression after chondrocytes were stimulated with IL-1β and CCL was applied, and to search for pathways that are affected by CCL.</div></div><div><h3>Results</h3><div>CCL of 40 kPa significantly upregulated the expression of IL-8, cyclooxygenase (COX)-2, nerve growth factor, matrix metalloproteinase (MMP)-1, and MMP-3. Transcription of IL-8, COX-2, and MMP-3 was synergistically promoted by CCL and IL-1β. The top 10 pathways enriched in the Kyoto Encyclopedia of Genes and Genomes enrichment analysis of differentially expressed genes were not common in either group, except for the “cytokine-cytokine receptor interaction”. The “tumor necrosis factor signaling pathway” and the “nuclear factor-kappa B signaling pathway” in the IL-1β group and “cell cycle” and the “Hippo signaling pathway” in the CCL group were included.</div></div><div><h3>Conclusions</h3><div>Comprehensive gene expression analysis revealed that CCL-induced changes in gene expression were different to those induced by stimulation with IL-1β. Our results provide new insights into the involvement of mechanical stimulation in the pathogenesis of OA.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100653"},"PeriodicalIF":2.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical, radiographic, and histological features of buccal bifurcation cysts: A systematic review to aid accurate diagnosis and treatment decisions","authors":"Matheus de Castro Costa ,&nbsp;Rani Kanthan ,&nbsp;Marina Lara de Carli ,&nbsp;Felipe Fornias Sperandio","doi":"10.1016/j.job.2025.100652","DOIUrl":"10.1016/j.job.2025.100652","url":null,"abstract":"<div><h3>Objective</h3><div>This systematic review delves into the nuanced landscape of buccal bifurcation cysts (BBCs), emphasizing their clinical significance amid the diagnostic challenges in oral and maxillofacial pathology. We trace the evolution of BBC classification from historical perspectives to its current status in the World Health Organization's classification system, aiming to equip dental professionals with crucial insights for accurate diagnosis and effective management.</div></div><div><h3>Methods</h3><div>This systematic review (PROSPERO: CRD42023405169) followed PRISMA guidelines to examine the epidemiological characteristics of BBCs. Observational studies were included, while reviews, meta-analyses, and experimental studies were excluded. A comprehensive search across five databases identified eligible studies. Two independent reviewers screened articles, resolving disagreements by consensus or a third reviewer. Data extraction included clinical, histological, and imaging findings. Risk of bias was assessed using Murad's framework for case reports/series and the Newcastle–Ottawa Scale for other study types, with studies rated as low, moderate, or high quality.</div></div><div><h3>Results</h3><div>The information presented here is crucial for preventing past treatment errors associated with BBC. In addition, this review confirms that BBCs predominantly affect the posterior mandible of pediatric patients and exhibit consistent clinical and histopathological features, aiding in their differentiation from similar maxillofacial lesions. Thus, well-informed clinicians should be able to diagnose BBC and make a proper treatment choice after familiarizing themselves with this review, which will ultimately lead to a favorable prognostic outcome and reduced risk of lesion recurrence.</div></div><div><h3>Conclusion</h3><div>This study provides a comprehensive analysis of BBC, aiming to enhance clinical understanding and ultimately improve patient care.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100652"},"PeriodicalIF":2.6,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}