Journal of Oral Biosciences最新文献

{"title":"O-GlcNAcase transiently translocates to the cytoplasm and regulates osteoblast differentiation","authors":"Xinyu Zheng ,&nbsp;Airi Tanai ,&nbsp;Heriati Sitosari ,&nbsp;Yao Weng ,&nbsp;Anggun Dwi Andini ,&nbsp;Koji Kimura ,&nbsp;Mika Ikegame ,&nbsp;Hirohiko Okamura ,&nbsp;Xiaohua Xie","doi":"10.1016/j.job.2025.100672","DOIUrl":"10.1016/j.job.2025.100672","url":null,"abstract":"<div><h3>Objectives</h3><div>O-GlcNAcylation is a reversible post-translational modification mediated by O-GlcNAcase (OGA) and O-GlcNAc transferase (OGT). Although localization of OGT during differentiation has been well studied, the spatial regulation and role of OGA in the maturation of osteoblasts remains unclear. This study investigated the translocation of OGA and its functional effects during the differentiation of osteoblasts.</div></div><div><h3>Methods</h3><div>Localization of OGA was assessed in mouse calvarial osteoblastic cells using immunohistochemistry and in pre-osteoblastic MC3T3-E1 cells using <em>in vitro</em> staining. OGA-knockout (OGA-KO) MC3T3-E1 cells were generated to evaluate differentiation using the osteogenic markers, Sp7, Dlx5, and Runx2, alkaline phosphatase (ALP) activity, and mineralization stains (von Kossa and Alizarin red).</div></div><div><h3>Results</h3><div>OGA was primarily cytoplasmic in osteoblastic cells of the mouse calvaria. In MC3T3-E1 cells, OGA was translocated from the nucleus to the cytoplasm by differentiation Day 3 and was stabilized by Day 6. OGA-KO cells had enhanced differentiation, increased ALP activity and mineralization, and upregulated Sp7 and Dlx5 expression. Immunohistochemistry showed that Sp7 mirrored the shift in localization of OGA, moving from the nucleus to the cytoplasm by Day 6, whereas Runx2 remained in the nucleus throughout differentiation.</div></div><div><h3>Conclusion</h3><div>Our findings reveal that dynamic translocation of OGA is a key event in early differentiation of osteoblasts that regulates maturation of osteoblasts. These insights suggest a novel regulatory role for OGA and identify potential targets for therapeutic strategies in the regeneration of bone.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 3","pages":"Article 100672"},"PeriodicalIF":2.6,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vascular inflammation and cancer malignancy","authors":"Yuya Sakurai ,&nbsp;Li Yu ,&nbsp;Aya Matsuda ,&nbsp;Nako Maishi ,&nbsp;Kyoko Hida","doi":"10.1016/j.job.2025.100671","DOIUrl":"10.1016/j.job.2025.100671","url":null,"abstract":"<div><h3>Background</h3><div>Vascular inflammation is a key contributor to cancer progression and metastasis. Tumor endothelial cells (TECs) respond to microbial, metabolic, and therapeutic stimuli by upregulating adhesion molecules and cytokines, which facilitates tumor cell adhesion and immune evasion.</div></div><div><h3>Highlight</h3><div>This review focuses on three representative vascular inflammatory triggers: <em>Streptococcus mutans</em>-induced endothelial activation, the oxLDL/LOX-1 signaling axis, and chemotherapy-induced vascular dysfunction. These mechanisms converge to establish a pre-metastatic niche. Emerging strategies including microbiota modulation, metabolic targeting, and low-dose metronomic (LDM) chemotherapy, have shown promise in preclinical studies for preserving vascular integrity and reducing inflammation.</div></div><div><h3>Conclusion</h3><div>Targeting vascular inflammation is a novel therapeutic approach to suppressing metastasis and cardiovascular events. Further studies are required to validate predictive biomarkers and optimize these strategies for clinical applications.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100671"},"PeriodicalIF":2.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide analyses of susceptibility genes responsible for mandibular prognathism in the Japanese population","authors":"Marie Hoshi-Numahata ,&nbsp;Atsuko Nakanishi-Kimura ,&nbsp;Haruhisa Watanabe ,&nbsp;Mai Nishiura ,&nbsp;Shinnosuke Nishimoto ,&nbsp;Fumi Ueno ,&nbsp;Riyu Koguchi ,&nbsp;Akira Oka ,&nbsp;Yoshiaki Sato ,&nbsp;Takashi S. Kajii ,&nbsp;Tadahiro Iimura","doi":"10.1016/j.job.2025.100670","DOIUrl":"10.1016/j.job.2025.100670","url":null,"abstract":"<div><h3>Background</h3><div>Mandibular prognathism (MP) is a type of malocclusion characterized by an imbalance in the anteroposterior position of the upper and lower jaws. The prevalence of MP in Japan is relatively high, suggesting a unique genetic background in the population.</div></div><div><h3>Highlight</h3><div>Genome-wide analyses identified susceptibility genes responsible for mandibular prognathism in the Japanese population.</div></div><div><h3>Conclusion</h3><div>Identification of the genes associated with malocclusion will pave the way for personalized and precise medicine and contribute to craniofacial biology.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100670"},"PeriodicalIF":2.6,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of pro-inflammatory cytokines induced by Porphyromonas gingivalis on cell cycle regulation in brain endothelial cells","authors":"Andrea Fernanda Rodríguez ,&nbsp;Juan Sebastian Buitrago ,&nbsp;Yormaris Castillo ,&nbsp;Gloria Inés Lafaurie ,&nbsp;Diana Marcela Buitrago-Ramirez","doi":"10.1016/j.job.2025.100668","DOIUrl":"10.1016/j.job.2025.100668","url":null,"abstract":"<div><h3>Objectives</h3><div>Advanced periodontitis potentially contributes to Alzheimer's disease (AD) development and progression by altering the blood–brain barrier microenvironment in the cerebral microvascular endothelium. This results, in cytotoxicity, cell cycle disruption, and increased pro-inflammatory cytokine expression, allowing pathogens to enter the brain and damage the central nervous system (CNS). This study evaluated the effects of <em>Porphyromonas gingivalis</em> W83 infection on pro-inflammatory response, cell viability, and cell cycle regulation in mouse brain endothelial cells (mBECs).</div></div><div><h3>Methods</h3><div>mBECs were stimulated with live <em>P. gingivalis</em> at different multiplicity of infection (MOI) values (1:5, 1:10, 1:50, 1:100, 1:200) for 6, 12, 24, and 48 h. Cell viability, cell cycle regulation, and pro-inflammatory cytokine mRNA expression were assessed using the alamarBlue assay, flow cytometry, and reverse transcription quantitative polymerase chain reaction (RT-qPCR), respectively.</div></div><div><h3>Results</h3><div><em>P. gingivalis</em> reduced cell viability, induced morphological changes in mBECs by &gt;50 % after 48 h (p &lt; 0.05) and caused concentration-dependent arrest in the S and G0/G1 phases of the cell cycle at MOI = 1:100 and 1:200. The <em>Il6</em>, <em>Il1b</em>, and tumor necrosis factor alpha (<em>Tnf</em>) mRNA expression increased significantly compared to that of the controls (p &lt; 0.05).</div></div><div><h3>Conclusions</h3><div><em>P. gingivalis</em> reduced cellular metabolism and induced early cell cycle arrest at the G0/G1 phase in mBECs cells. It also increased the pro-inflammatory response, which could be associated with cell death and possible senescence of brain endothelial cells. These results suggested a possible role for <em>P. gingivalis</em> in the pathogenesis of AD. Further studies are required to elucidate these underlying mechanisms.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100668"},"PeriodicalIF":2.6,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of electrical stimulation for antimicrobial capacity of titanium materials implants: A systematic review and meta-analysis","authors":"Simone Kreve,&nbsp;Andréa C. dos Reis","doi":"10.1016/j.job.2025.100669","DOIUrl":"10.1016/j.job.2025.100669","url":null,"abstract":"<div><h3>Background</h3><div>Antimicrobial resistance undermines the effectiveness of drugs for treating implant-associated infections. Consequently, there is growing interest in identifying alternative methods to prevent and eliminate infections. The aim of this systematic review was to ascertain whether the electrical stimulation of titanium implants or titanium-based implant materials has antimicrobial properties against bacterial biofilms. The search was conducted in various databases, including PubMed/Medline, Web of Science, EMBASE, SCOPUS, and Google Scholar, in February 2024. In addition, a manual search of the reference lists of the included articles was conducted. The eligibility criteria included in vivo and in vitro studies evaluating the effects of electrical stimulation on titanium implants or titanium-based implant materials in reducing biofilm formation or adhesion as well as eradicating or reducing the viability of bacterial biofilms. The variability between studies was determined using the inverse variance method with random- and fixed-effects models. Heterogeneity was assessed using the I2 and prediction interval statistics. Publication bias was qualitatively evaluated using funnel plots.</div></div><div><h3>Highlights</h3><div>Different electrical stimulation (ES) parameters (current and voltage) exhibited antibacterial activity, resulting in either bacteriostatic or bactericidal effects.</div></div><div><h3>Conclusions</h3><div>ES in titanium or titanium-based implant materials confers antimicrobial capacity against bacterial biofilms, and its effectiveness depends on the applied tension. The association between ES and antimicrobials was more robust than with ES administered individually.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100669"},"PeriodicalIF":2.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of glucose metabolism in amelogenesis","authors":"Hiroko Ida-Yonemochi","doi":"10.1016/j.job.2025.100667","DOIUrl":"10.1016/j.job.2025.100667","url":null,"abstract":"<div><h3>Background</h3><div>Cell energy metabolism plays a pivotal role in organ development and function by regulating cell behavior in pathophysiological conditions. Glucose metabolism is the central cascade for obtaining energy in mammalian cells, and cells alter the glucose metabolic pathway depending on intra- and extracellular environments. Therefore, glucose metabolism is closely associated with cell differentiation stages, and cell energy metabolism plays a vital role not only in energy production but also in cell fate regulation in organogenesis.</div></div><div><h3>Highlight</h3><div>During enamel formation, the timing of the expression of passive and active glucose transporters, glycogen synthesis, and glycogen degradation is strictly regulated according to the energy demand of ameloblast-lineage cells. These glucose metabolic reactions are particularly active in the maturation stage of ameloblasts. Furthermore, autophagy, a key regulator of cellular energy homeostasis that modulates glucose metabolism, occurs during both the secretory and maturation stages of ameloblasts. Disruption of glucose metabolism cascade and autophagy induces enamel hypoplasia, as demonstrated in both <em>in vitro</em> and <em>in vivo</em> models.</div></div><div><h3>Conclusion</h3><div>Adequate energy supply via glucose metabolism is essential for enamel matrix secretion and maturation. A thorough understanding of the precise regulation of energy metabolism in amelogenesis facilitates comprehension of the normal enamel formation process and pathological conditions affecting it. This review summarizes glucose metabolic processes during amelogenesis, focusing on glucose uptake, glycogenesis, and glycogenolysis.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100667"},"PeriodicalIF":2.6,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemokine receptor 5 signaling in oral diseases and degenerative temporomandibular joint disease","authors":"Haruhisa Watanabe ,&nbsp;Riyu Koguchi ,&nbsp;Takashi S. Kajii ,&nbsp;Yutaka Maruoka ,&nbsp;Tadahiro Iimura","doi":"10.1016/j.job.2025.100666","DOIUrl":"10.1016/j.job.2025.100666","url":null,"abstract":"<div><h3>Background</h3><div>Chemokine receptor 5 (CCR5)-mediated signals are involved in various biological responses and inflammatory diseases. Recent studies have revealed the roles of this signaling pathway in bone metabolism, metabolic bone diseases, and joint diseases.</div></div><div><h3>Highlight</h3><div>Through preclinical and clinical studies, our research group has demonstrated that CCR5 signaling is deeply involved in degenerative changes in the temporomandibular joint (TMJ).</div></div><div><h3>Conclusion</h3><div>In this short review, we outline the diverse functions of CCR5 signaling in oral and degenerative TMJ diseases.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100666"},"PeriodicalIF":2.6,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neisseria perflava isolated from a clinical sample reduces influenza virus replication in respiratory cells","authors":"Keisuke Nishioka ,&nbsp;Maki Nakagawa ,&nbsp;Yoko Tanino ,&nbsp;Takaaki Nakaya","doi":"10.1016/j.job.2025.100665","DOIUrl":"10.1016/j.job.2025.100665","url":null,"abstract":"<div><h3>Objectives</h3><div>Various bacteria are present in the oral cavity and constitute the oral microbiota. Although the oral microbiota has been analyzed using next-generation sequencing, few studies have investigated whether specific commensal bacteria directly affect immune responses to infections. Here, we focused on <em>Neisseria</em> species present in the oral cavity and investigated their effects on respiratory cells infected with several viruses.</div></div><div><h3>Methods</h3><div>Six <em>Neisseria</em> species were isolated from human saliva. The epithelial cell lines were stimulated with bacterial culture supernatants before viral infection. Changes in the viral susceptibility were assessed.</div></div><div><h3>Results</h3><div>Culture supernatants of two <em>Neisseria</em> species were found to affect cells susceptible to influenza viral infection and suppress influenza viral replication. The mechanism underlying the suppression of <em>N. perflava</em> was further investigated. This activity was observed in the 10–30 kDa protein range fractionated by ultrafiltration. Although viral replication was suppressed by stimulation with bacterial proteins, the infection efficiency of the virus and cytokine production were unaffected. Replication of SARS-CoV-2 and human rhinovirus were also suppressed.</div></div><div><h3>Conclusion</h3><div>Viral infection was performed after supernatant stimulation, suggesting that exposure to oral bacteria directly affects viral infection in the surrounding cells. This effect has been observed for several viruses. Viral genome replication in cells may be suppressed by enhanced expression of viral replication suppression genes. Further analyses are required to elucidate the detailed underlying mechanisms.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100665"},"PeriodicalIF":2.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143869412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histochemical assessment of the anabolic effects of abaloparatide on the femoral metaphyses of ovariectomized mice","authors":"Xuanyu Liu ,&nbsp;Tomoka Hasegawa ,&nbsp;Mako Sakakibara ,&nbsp;Tomomaya Yamamoto ,&nbsp;Mai Haraguchi-Kitakamae ,&nbsp;Hotaka Ishizu ,&nbsp;Yan Shi ,&nbsp;Jiaxin Cui ,&nbsp;Weisong Li ,&nbsp;Wang Haoyu ,&nbsp;Hiromi Hongo ,&nbsp;Tomohiro Shimizu ,&nbsp;Yoichi Ohiro ,&nbsp;Norio Amizuka","doi":"10.1016/j.job.2025.100663","DOIUrl":"10.1016/j.job.2025.100663","url":null,"abstract":"<div><h3>Objective</h3><div>To clarify the mechanism of bone anabolism induced by the parathyroid hormone-related peptide analog abaloparatide, we histochemically examined the femora of ovariectomized mice treated with abaloparatide.</div></div><div><h3>Methods</h3><div>Twelve-week-old female C57BL/6J mice underwent ovariectomies (OVX), and were then administered either abaloparatide (30 μg/kg/day: OVX + ABL group) or vehicle (OVX group) via daily intraperitoneal injection. Femora were harvested at 0, 2, 4, and 6 weeks post-administration and subjected to micro-CT imaging, TRAP, cathepsin K, ALP, and PHOSPHO1 staining, along with calcein labeling.</div></div><div><h3>Results</h3><div>In the OVX group, trabecular number and bone volume gradually decreased over time, whereas the OVX + ABL group maintained these values to 6 weeks after OVX. The numbers of TRAP-positive/cathepsin K-reactive osteoclasts per bone surface area were similar between the OVX and OVX + ABL group, except for a temporary increase at 4 weeks in the OVX group. In the OVX group, the areas of ALP-positive osteoblastic cells and PHOSPHO1-reactive mature osteoblasts decreased, whereas in the OVX + ABL group, ALP-positive osteoblastic cells surrounded the trabeculae, and long lines of PHOSPHO1-reactive mature osteoblasts expanded to the terminal region of the trabeculae. In addition, long continuous calcein labeling was seen on slightly convex new bone, indicating modeling-based bone formation in the OVX + ABL group. The bone formation rate/bone surface ratio and the total length of modeling-based bone formation sites were higher in the OVX + ABL group than in the OVX group.</div></div><div><h3>Conclusion</h3><div>Abaloparatide suppresses bone loss following ovariectomy by promoting both remodeling-based and modeling-based bone formation.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100663"},"PeriodicalIF":2.6,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143894619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of PilX, pilus component of Streptococcus sanguinis","authors":"Li Yixuan ,&nbsp;Masanobu Nakata ,&nbsp;Hirono Migita ,&nbsp;Airi Matsumoto ,&nbsp;Yuichi Oogai ,&nbsp;Katsuki Takebe ,&nbsp;Masaya Yamaguchi ,&nbsp;Nobuo Okahashi ,&nbsp;Tomoko Sumitomo ,&nbsp;Shigetada Kawabata","doi":"10.1016/j.job.2025.100664","DOIUrl":"10.1016/j.job.2025.100664","url":null,"abstract":"<div><h3>Objectives</h3><div><em>Streptococcus sanguinis</em> is an oral commensal bacterium that promotes dental biofilm formation and causes infective endocarditis. <em>S. sanguinis</em> strain SK36 produces pili comprising PilA, PilB, and PilC. This study determined whether the <em>ssa</em>1635 gene adjacent to the pilus-related gene locus encodes a pilus component and its roles in biofilm formation and eukaryotic cell adhesion.</div></div><div><h3>Methods</h3><div>Using a series of mutant strains and antisera against PilA, PilB, PilC, and SSA1635, immunoblot analyses and immunoprecipitation assays were performed for SSA1635 characterization. Both involvement of the deduced pilus-specific transpeptidase SrtC in pilus assembly and SSA1635 localization were examined by immunoblot analysis of various mutant strains. Furthermore, biofilm formation assays on saliva-coated surfaces and adhesion to HeLa cells were conducted to assess functions.</div></div><div><h3>Results</h3><div>SSA1635, designated as PilX, formed complexes with PilA, PilB, and PilC. PilX was identified as a tip pilin incorporated into the pilus structure by SrtC. Notably, deletion of <em>pilX</em> impaired polymerization of other pilins. Furthermore, a <em>pilX</em> deletion mutant exhibited decreased biofilm formation compared with the wild-type and revertant strains and comparable rates of adherence to HeLa cells.</div></div><div><h3>Conclusions</h3><div>PilX is a potential pilin tip that may aid in facilitating the polymerization of other pilins. PilX contributes to biofilm formation, although it appears to be dispensable for adhesion to HeLa cells. Further characterization of PilX-binding specificities will provide valuable insights into the colonization mechanism of <em>S. sanguinis</em>.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100664"},"PeriodicalIF":2.6,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}