Journal of Oral Biosciences最新文献_第2页

Anti-Streptococcus mutans and anti-inflammatory effects of ginsenoside Compound K and enzyme-treated red ginseng extract (BTEX-K). 人参皂苷化合物 K 和酶处理红参提取物（BTEX-K）的抗变异链球菌和抗炎作用。

IF 2.6

Journal of Oral Biosciences Pub Date : 2024-08-09 DOI: 10.1016/j.job.2024.08.001

Jin-Hwan Oh, SangJoon Mo, Le Thi Nhu Ngoc, Jonghyuk Lee, Moon-Young Kim, Hae-Seo Park, Jin-Hee Kim, Yu-Jin Ha, Lee Sung, Young-Chul Lee, Youl Hour

{"title":"Anti-Streptococcus mutans and anti-inflammatory effects of ginsenoside Compound K and enzyme-treated red ginseng extract (BTEX-K).","authors":"Jin-Hwan Oh, SangJoon Mo, Le Thi Nhu Ngoc, Jonghyuk Lee, Moon-Young Kim, Hae-Seo Park, Jin-Hee Kim, Yu-Jin Ha, Lee Sung, Young-Chul Lee, Youl Hour","doi":"10.1016/j.job.2024.08.001","DOIUrl":"https://doi.org/10.1016/j.job.2024.08.001","url":null,"abstract":"<p><strong>Objectives: </strong>Dental caries, or tooth decay, is an oral health issue worldwide. Oral healthcare researchers are considering how to develop safe and effective preventive measures and treatments for dental caries. This study evaluated the potential applications of Compound K and BTEX-K, a Compound K-rich red ginseng extract, for the prevention and treatment of dental caries. Moreover, this study briefly confirmed its inhibitory effect on inflammation, an important factor in dental health.</p><p><strong>Methods: </strong>The amount of organic acids produced by bacteria in biofilm was determined using in vitro and in vivo assays. The ability of these extracts to promote tooth remineralization and microhardness was evaluated using an in vivo mouse assay. We evaluated their anti-inflammatory potential by inhibiting proinflammatory cytokine expression and lipopolysaccharide-induced nitrous oxide production in cell lines.</p><p><strong>Results: </strong>Compound K (10-20 μg/mL) and BTEX-K (50-100 μg/mL) effectively inhibited the growth of Streptococcus mutans bacteria, demonstrating significant antibacterial properties. They can potentially prevent biofilm formation by reducing lactic acid production in the teeth. These compounds showed a strong ability to promote tooth remineralization and improve the microhardness of acid-producing bacteria. They also possess potent anti-inflammatory properties that downregulate proinflammatory cytokine (interleukin-6, interleukin-1β, inducible nitric oxide synthase) expression, suppress nuclear factor-kappa B transcription factor activation (∼1.6 times), and reduce nitrous oxide production in lipopolysaccharide-induced RAW264.7 cells.</p><p><strong>Conclusions: </strong>Compounds K and BTEX-K may provide a novel approach to dental caries prevention as well as inflammation prevention and treatment.</p>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141917734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular microbiological profiling of bottled unsweetened tea beverages: A screening experiment 瓶装不加糖茶饮料的分子微生物分析：筛选实验。

IF 2.6

Journal of Oral Biosciences Pub Date : 2024-07-26 DOI: 10.1016/j.job.2024.07.006

引用次数: 0

Therapeutic Effects of Mesenchymal Stem Cells Derived from Bone Marrow and Adipose Tissue in a Rat Model of Temporomandibular Osteoarthritis. 骨髓和脂肪组织间充质干细胞对颞下颌关节炎大鼠模型的治疗效果

IF 2.6

Journal of Oral Biosciences Pub Date : 2024-07-24 DOI: 10.1016/j.job.2024.07.007

Saba Khazeni, Mohammadali Ghavimi, Mehran Mesgari-Abbasi, Leila Roshangar, Sara Abedi, Tannaz Pourlak

{"title":"Therapeutic Effects of Mesenchymal Stem Cells Derived from Bone Marrow and Adipose Tissue in a Rat Model of Temporomandibular Osteoarthritis.","authors":"Saba Khazeni, Mohammadali Ghavimi, Mehran Mesgari-Abbasi, Leila Roshangar, Sara Abedi, Tannaz Pourlak","doi":"10.1016/j.job.2024.07.007","DOIUrl":"10.1016/j.job.2024.07.007","url":null,"abstract":"<p><strong>Objectives: </strong>To examine the potential of intra-articular administration of mesenchymal stem cells (MSCs) derived from bone marrow or adipose tissue to mitigate synovial inflammation in a rat model of temporomandibular joint (TMJ) osteoarthritis (OA).</p><p><strong>Methods: </strong>In this experimental study, 40 rats were divided into 4 groups: (1) Control group; (2) Untreated TMJ-OA group; (3) TMJ-OA group treated with bone marrow-derived MSCs; (4) TMJ-OA group treated with adipose tissue-derived MSCs. The TMJ-OA model was established by inducing synovial inflammation through the intra-articular administration of complete Freund's adjuvant (CFA). After 8 weeks of TMJ-OA establishment, the animals were sacrificed and each mandibular condyle was extracted for histological evaluation.</p><p><strong>Results: </strong>The untreated TMJ-OA group had significantly higher synovial inflammation, as indicated microscopically by higher grades of synovial membrane hyperplasia and adhesion, vascular vasodilation, and fibrin deposition than the control group (p < 0.001). Both TMJ-OA groups treated with MSCs had lower grades of synovial inflammation and less severe synovitis than the untreated TMJ-OA group (p < 0.001). The TMJ-OA group treated with adipose tissue-derived MSCs showed lower grades of synovial membrane hyperplasia and higher grades of fibrin deposition than the that treated with bone marrow-derived MSCs (p < 0.001). Other indicators of synovial inflammation and synovitis severity were comparable between the two treatment groups.</p><p><strong>Conclusions: </strong>Administration of CFA to the TMJ-OA rat model augmented synovial inflammation. Intra-articular administration of MSCs derived from either bone marrow or adipose tissue attenuated the microscopic manifestations of this inflammation, indicating the therapeutic potential of this treatment for TMJ-OA.</p>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141767628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytoarchitecture and intercellular interactions in the trigeminal ganglion: Associations with neuropathic pain in the orofacial region 三叉神经节的细胞结构和细胞间相互作用：与口面部神经性疼痛的关联

IF 2.6

Journal of Oral Biosciences Pub Date : 2024-07-18 DOI: 10.1016/j.job.2024.07.003

{"title":"Cytoarchitecture and intercellular interactions in the trigeminal ganglion: Associations with neuropathic pain in the orofacial region","authors":"","doi":"10.1016/j.job.2024.07.003","DOIUrl":"10.1016/j.job.2024.07.003","url":null,"abstract":"<div><h3>Background</h3><p>Disorders of the trigeminal nerve, a sensory nerve of the orofacial region, often lead to complications in dental practice, including neuropathic pain, allodynia, and ectopic pain. Management of these complications requires an understanding of the cytoarchitecture of the trigeminal ganglion, where the cell bodies of the trigeminal nerve are located, and the mechanisms of cell-cell interactions.</p></div><div><h3>Highlights</h3><p>In the trigeminal ganglion, ganglion, satellite, Schwann, and immune cells coexist and interact. Cell-cell interactions are complex and occur through direct contact via gap junctions or through mediators such as adenosine triphosphate, nitric oxide, peptides, and cytokines. Interactions between the nervous and immune systems within the trigeminal ganglion may have neuroprotective effects during nerve injury or may exacerbate inflammation and produce chronic pain. Under pathological conditions of the trigeminal nerve, cell-cell interactions can cause allodynia and ectopic pain. Although cell-cell interactions that occur via mediators can act at some distance, they are more effective when the cells are close together. Therefore, information on the three-dimensional topography of trigeminal ganglion cells is essential for understanding the pathophysiology of ectopic pain.</p></div><div><h3>Conclusions</h3><p>A three-dimensional map of the somatotopic localization of trigeminal ganglion neurons revealed that ganglion cells innervating distant orofacial regions are often apposed to each other, interacting with and potentially contributing to ectopic pain. Elucidation of the complex network of mediators and their receptors responsible for intercellular communication within the trigeminal ganglion is essential for understanding ectopic pain.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1349007924001518/pdfft?md5=03deb21023fdafb0f2ce165d9d73cd9f&pid=1-s2.0-S1349007924001518-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-neuronal cells act as crucial players in neuropathic orofacial pain 非神经元细胞是导致神经性口面痛的关键因素。

IF 2.6

Journal of Oral Biosciences Pub Date : 2024-07-18 DOI: 10.1016/j.job.2024.07.005

{"title":"Non-neuronal cells act as crucial players in neuropathic orofacial pain","authors":"","doi":"10.1016/j.job.2024.07.005","DOIUrl":"10.1016/j.job.2024.07.005","url":null,"abstract":"<div><h3>Background</h3><p><span>Following peripheral nerve damage, various non-neuronal cells are activated, triggering accumulation in the peripheral and </span>central nervous systems<span>, and communicate with neurons. Evidence suggest that neuronal and non-neuronal cell communication is a critical mechanism of neuropathic pain<span>; however, its detailed mechanisms in contributing to neuropathic orofacial pain development remain unclear.</span></span></p></div><div><h3>Highlight</h3><p><span><span><span>Neuronal and non-neuronal cell communication in the trigeminal ganglion (TG) is believed to cause neuronal </span>hyperactivation following </span>trigeminal nerve damage, resulting in neuropathic orofacial pain. Trigeminal nerve damage activates and accumulates non-neuronal cells, such as satellite cells and macrophages in the TG and </span>microglia<span><span>, astrocytes, and oligodendrocytes<span> in the trigeminal spinal subnucleus caudalis (Vc) and upper cervical spinal cord (C1–C2). These non-neuronal cells release various molecules, contributing to the hyperactivation of TG, Vc, and C1–C2 nociceptive neurons. These hyperactive nociceptive neurons release molecules that enhance non-neuronal </span></span>cell activation. This neuron and non-neuronal cell crosstalk causes hyperactivation of nociceptive neurons in the TG, Vc, and C1–C2. Here, we addressed previous and recent data on the contribution of neuronal and non-neuronal cell communication and its involvement in neuropathic orofacial pain development.</span></p></div><div><h3>Conclusion</h3><p>Previous and recent data suggest that neuronal and non-neuronal cell communication in the TG, Vc, and C1–C2 is a key mechanism that causes neuropathic orofacial pain associated with trigeminal nerve damage.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Platelet-rich fibrin as an adjunct to scaling and root planing in treatment of shallow periodontal pockets: A randomized clinical trial 富血小板纤维蛋白辅助洗牙和根面平整治疗浅牙周袋：随机临床试验。

IF 2.6

Journal of Oral Biosciences Pub Date : 2024-07-10 DOI: 10.1016/j.job.2024.07.002

{"title":"Platelet-rich fibrin as an adjunct to scaling and root planing in treatment of shallow periodontal pockets: A randomized clinical trial","authors":"","doi":"10.1016/j.job.2024.07.002","DOIUrl":"10.1016/j.job.2024.07.002","url":null,"abstract":"<div><h3>Objectives</h3><p>To evaluate the efficacy of platelet-rich fibrin (PRF) as an adjunct to scaling and root planing<span> (ScRp) for healing shallow periodontal pockets.</span></p></div><div><h3>Methods</h3><p>Twelve patients with periodontitis<span><span><span> were enrolled in this split-mouth, randomized clinical trial<span><span><span>. A total of 24 shallow periodontal pockets (4–6 mm) were treated by either ScRp alone (control) or PRF (test). </span>Clinical attachment loss (CAL), probing pocket depth (PPD), </span>bleeding on probing (BOP), and </span></span>plaque index (PLI), as well as platelet-derived growth factor-BB (PDGF-BB) by enzyme-linked immunosorbent assay (ELISA) in </span>gingival crevicular fluid (GCF) were measured at baseline and at 1- and 3-month follow-up visits.</span></p></div><div><h3>Results</h3><p>At 1- and 3-month follow-up visits, greater CAL gains (2.6 ± 0.25 mm and 3.26 ± 0.31 mm, respectively) and PPD reductions (2.58 ± 0.38 and 3.31 ± 0.39 mm, respectively) were observed in the test group compared to those in controls (CAL gain of 1.01 ± 0.49 mm and 1.43 ± 0.48 mm; PPD reduction of 1.1 ± 0.55 and 1.37 ± 0.49 mm, respectively). In addition, the increase in PDGF-BB in GCF in the test group (724.5 ± 186.09 pg/μl and 1957.5 ± 472.9 pg/μl) was significantly greater than that in controls (109.3 ± 24.07 and 614.64 ± 209.3 pg/μl) at 1- and 3-month follow-up visits, respectively.</p></div><div><h3>Conclusions</h3><p>The noninvasive use of PRF as an adjunct to ScRp successfully improved clinical periodontal parameters and might contribute to increased PDGF-BB in GCF.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141601885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intracellular Signaling Pathways Involved in the Regulation of Gene Expression by Pilocarpine. 参与皮洛卡品基因表达调控的细胞内信号通路

IF 2.6

Journal of Oral Biosciences Pub Date : 2024-07-09 DOI: 10.1016/j.job.2024.07.004

Hirohito Sakazume, Takao Morita, Haruka Yamaguchi, Akira Tanaka

{"title":"Intracellular Signaling Pathways Involved in the Regulation of Gene Expression by Pilocarpine.","authors":"Hirohito Sakazume, Takao Morita, Haruka Yamaguchi, Akira Tanaka","doi":"10.1016/j.job.2024.07.004","DOIUrl":"https://doi.org/10.1016/j.job.2024.07.004","url":null,"abstract":"<p><strong>Objectives: </strong>Pilocarpine is commonly used clinically to treat dry mouth. The long-term administration of pilocarpine reportedly improves salivary secretion more effectively than short-term administration. Therefore, we hypothesized that pilocarpine alters gene expression in salivary glands via muscarinic receptor stimulation. This study aimed to investigate the effects of pilocarpine use on gene expression mediated by mitogen-activated protein kinase (MAPK) activity.</p><p><strong>Methods: </strong>The effects of pilocarpine on gene expression were investigated in rats and human salivary gland (HSY) cells using several inhibitors of intracellular signaling pathways. Gene expression in the rat submandibular gland and HSY cells was determined using reverse transcription-quantitative polymerase chain reaction analysis of total RNA.</p><p><strong>Results: </strong>In animal experiments, at 7 days after pilocarpine stimulation, Ctgf and Sgk1 expressions were increased in the submandibular gland. In cell culture experiments, pilocarpine increased Ctgf expression in HSY cells. The mitogen-activated protein kinase kinase inhibitor trametinib, the Src inhibitor PP2, and the muscarinic acetylcholine receptor antagonist atropine suppressed the effect of pilocarpine on gene expression.</p><p><strong>Conclusions: </strong>Pilocarpine enhances Ctgf and Sgk1 expressions by activating Src-mediated MAPK activity. Although further studies are required to fully understand the roles of Ctgf and Sgk1, changes in gene expression may play an important role in improving salivary secretions.</p>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141591671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dipotassium glycyrrhizate prevents oral dysbiosis caused by Porphyromonas gingivalis in an in vitro saliva-derived polymicrobial biofilm model 在体外唾液衍生多微生物生物膜模型中，甘草酸二钾可预防牙龈卟啉单胞菌引起的口腔菌群失调。

IF 2.6

Journal of Oral Biosciences Pub Date : 2024-07-05 DOI: 10.1016/j.job.2024.07.001

{"title":"Dipotassium glycyrrhizate prevents oral dysbiosis caused by Porphyromonas gingivalis in an in vitro saliva-derived polymicrobial biofilm model","authors":"","doi":"10.1016/j.job.2024.07.001","DOIUrl":"10.1016/j.job.2024.07.001","url":null,"abstract":"<div><h3>Objectives</h3><p><span><span>Oral microbiome </span>dysbiosis<span><span> prevention is important to avoid the onset and progression of periodontal disease. Dipotassium glycyrrhizate (GK2) is a </span>licorice root<span> extract with anti-inflammatory effects, and its associated mechanisms have been well-reported. However, their effects on the oral microbiome<span> have not been investigated. This study aimed to elucidate the effects of GK2 on the oral microbiome using an </span></span></span></span><em>in vitro</em> polymicrobial biofilm model.</p></div><div><h3>Methods</h3><p>An <em>in vitro</em><span><span><span> saliva-derived polymicrobial biofilm model was used to evaluate the effects of GK2 on the oral microbiome. One-week anaerobic culture was performed, in which GK2 was added to the medium. Subsequently, microbiome analysis was performed based on the V1–V2 region of the 16 S </span>rRNA gene, and </span>pathogenicity indices were assessed. We investigated the effects of GK2 on various bacterial monocultures by evaluating its inhibitory effects on cell growth, based on culture turbidity.</span></p></div><div><h3>Results</h3><p>GK2 treatment altered the microbiome structure and decreased the relative abundance of periodontal pathogenic bacteria, including <span><span>Porphyromonas</span></span><span><span>. Moreover, GK2 treatment reduced the DPP4 activity —a pathogenicity<span> index of periodontal disease. Specifically, GK2 exhibited selective </span></span>antibacterial activity against periodontal pathogenic bacteria.</span></p></div><div><h3>Conclusions</h3><p><span>These findings suggest that GK2 has a selective antibacterial effect against periodontal pathogenic bacteria; thus, preventing oral microbiome dysbiosis. Therefore, GK2 is expected to contribute to </span>periodontal disease prevention by modulating the oral microbiome toward a state with low inflammatory potential, thereby utilizing its anti-inflammatory properties on the host.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of anti-vascular endothelial growth factor antibody restores the function of saliva secretion in a type 2 diabetes mouse model 应用抗血管内皮生长因子抗体恢复 2 型糖尿病小鼠模型的唾液分泌功能。

IF 2.6

Journal of Oral Biosciences Pub Date : 2024-06-27 DOI: 10.1016/j.job.2024.06.011

{"title":"Application of anti-vascular endothelial growth factor antibody restores the function of saliva secretion in a type 2 diabetes mouse model","authors":"","doi":"10.1016/j.job.2024.06.011","DOIUrl":"10.1016/j.job.2024.06.011","url":null,"abstract":"<div><h3>Objectives</h3><p><span><span><span>Xerostomia<span><span>, a common complication of type 2 diabetes, leads to an increased risk of caries, </span>dysphagia, and </span></span>dysgeusia. Although anti-vascular endothelial growth factor (VEGF) antibodies, such as </span>ranibizumab (RBZ), have been used to treat </span>diabetic retinopathy<span>, their effects on the salivary glands<span> are unknown. This study evaluated the effects of RBZ on salivary glands<span> to reduce inflammation and restore salivary function in a mouse model of type 2 diabetes.</span></span></span></p></div><div><h3>Methods</h3><p>Male KK-A<sup>y</sup><span> mice with type 2 diabetes (10–12 weeks old) were used. The diabetes mellitus (DM) group received phosphate-buffered saline, while the DM + RBZ group received an intraperitoneal administration of RBZ (100 μg/kg) 24 h before the experiment.</span></p></div><div><h3>Results</h3><p><span><span>Ex vivo</span></span><span><span> perfusion experiments showed a substantial increase in salivary secretion<span> from the submandibular gland<span><span><span> (SMG) in the DM + RBZ group. In addition, the mRNA expression levels of TNF-α and IL-1β were considerably lower in this group. In contrast, those of </span>aquaporin 5 were substantially higher in the DM + RBZ group, as revealed by </span>quantitative reverse transcription PCR. Furthermore, the number of </span></span></span>lymphocyte infiltration spots in the SMG was notably lower in the DM + RBZ group. Finally, intracellular Ca</span><sup>2+</sup><span> signaling in acinar cells was considerably higher in the DM + RBZ group than that in the DM group.</span></p></div><div><h3>Conclusion</h3><p>Treating a type 2 diabetic mouse model with RBZ restored salivary secretion through its anti-inflammatory effects.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141471487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regional difference in the distribution of alkaline phosphatase, PHOSPHO1, and calcein labeling in the femoral metaphyseal trabeculae in parathyroid hormone-administered mice 服用甲状旁腺激素的小鼠股骨骺小梁中碱性磷酸酶、PHOSPHO1和钙蓝蛋白标记分布的区域差异。

IF 2.6

Journal of Oral Biosciences Pub Date : 2024-06-26 DOI: 10.1016/j.job.2024.06.007

{"title":"Regional difference in the distribution of alkaline phosphatase, PHOSPHO1, and calcein labeling in the femoral metaphyseal trabeculae in parathyroid hormone-administered mice","authors":"","doi":"10.1016/j.job.2024.06.007","DOIUrl":"10.1016/j.job.2024.06.007","url":null,"abstract":"<div><h3>Objectives</h3><p>This study aimed to elucidate whether the administration of parathyroid hormone<span> (PTH) results in remodeling- or modeling-based bone formation in different regions of the murine femora, and whether the PTH-driven bone formation would facilitate osteoblastic differentiation into osteocytes.</span></p></div><div><h3>Methods</h3><p>Six-week-old male C57BL/6J mice were employed to examine the distribution of alkaline phosphatase<span><span> (ALP), PHOSPHO1, podoplanin, and </span>calcein<span> labeling in two distinct long bone regions: the metaphyseal trabeculae close to the chondro-osseous junction (COJ) and those distant from the COJ in three mouse groups, a control group receiving a vehicle (sham group) and groups receiving hPTH (1–34) twice a day (PTH BID group) or four times a day (PTH QID group) for two weeks.</span></span></p></div><div><h3>Results</h3><p><span><span>The sham group showed PHOSPHO1-reactive mature osteoblasts localized primarily at the COJ, whereas the PTH BID/QID groups exhibited extended lines of PHOSPHO1-reactive osteoblasts even in regions distant from the COJ. The PTH QID group displayed fragmented </span>calcein<span> labeling in trabeculae close to the COJ, whereas continuous labeling was observed in trabeculae distant from the COJ. Osteoblasts tended to express podoplanin and PHOSPHO1 independently in the close and distant regions of the sham group, while osteoblasts in the PTH-administered groups showed </span></span>immunoreactivity of podoplanin and PHOSPHO1 together in the close and distant regions.</p></div><div><h3>Conclusions</h3><p>Administration of PTH may accelerate remodeling-based bone formation in regions close to the COJ while predominantly inducing modeling-based bone formation in distant regions. PTH appeared to simultaneously facilitate osteoblastic bone mineralization<span> and differentiation into osteocytes in both remodeling- and modeling-based bone formation.</span></p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141471491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0