Journal of Oral Biosciences最新文献

{"title":"Growing lesser horn of the hyoid in human fetuses with special references to muscles attaching to it","authors":"Jose Francisco Rodríguez-Vázquez ,&nbsp;Kenta Abe ,&nbsp;Kazuma Morita ,&nbsp;Yuki Yoshihashi ,&nbsp;Masahito Yamamoto ,&nbsp;Shin-ichi Abe","doi":"10.1016/j.job.2026.100733","DOIUrl":"10.1016/j.job.2026.100733","url":null,"abstract":"<div><h3>Objectives</h3><div>The hyoid lesser horn (LH) forms a transient joint with the greater horn in human fetuses. The chondroglossus muscle attaches to the LH; however, its morphology remains unclear. We hypothesized that the muscles involved in LH accelerate joint formation.</div><div>Methods: We examined 34 human fetuses with crown-rump length of 39–230 mm.</div></div><div><h3>Results</h3><div>The LH joint was bilaterally absent in eight fetuses; the largest specimen “without” joints was 110 mm crown-rump length, whereas the smallest specimen “with” joints was 55 mm. The chondroglossus was identified as oblique muscle fibers attached to the LH medial aspect (23/34). The chondroglossus, Adjacent to the transverse lingual muscle (TLM), the chondroglossus ran supero-antero-medially to join the genioglossus. The TLM (21/34) and middle pharyngeal constrictor (pars chondropharyngica; 21/34) originated from the LH. The latter ran posteriorly from the LH medial to the hypoglossal nerve, whereas the middle constrictor arose largely from the greater horn. Notably, without LH attachment, the anterior margin of the mylohyoideus muscle (MPCM) was connected to the TLM using thick fascia wrapped around the LH. Therefore, LH disrupts the muscular ring surrounding the oropharynx.</div></div><div><h3>Conclusions</h3><div>The variation in joint formation timing (9–16 weeks gestational age) suggests that the chondroglossus, TLM, and MPCM development do not require joint movement. Alternatively, an elongated greater horn may provide joint-like space.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"68 1","pages":"Article 100733"},"PeriodicalIF":2.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145976195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral biosciences: The annual review 2025","authors":"Hayato Ohshima ,&nbsp;Kentaro Ono ,&nbsp;Kenji Mishima","doi":"10.1016/j.job.2026.100750","DOIUrl":"10.1016/j.job.2026.100750","url":null,"abstract":"<div><h3>Background</h3><div>The <em>Journal of Oral Biosciences</em> is dedicated to advancing and disseminating fundamental knowledge across the full spectrum of oral biosciences. This editorial features recently published review articles spanning key domains, including “craniofacial biology”; “bone, tooth, and mineral biology”; “periodontal and pulp biology”; “microbiology and immunology”; “pharmacology”; “biomaterials”; “oncology”; “salivary research”; and “regenerative medicine.”</div></div><div><h3>Highlight</h3><div>The featured review articles address a diverse range of topics, including buccal bifurcation cysts, mandibular prognathism, glucose metabolism, gingival overgrowth, dextrins, chemokine receptor 5, electrical stimulation, the tumor microenvironment, computer-aided diagnosis, adiponectin, myoepithelial cells, secretory granules, apoptosis, dental regenerative medicine, and three-dimensional <em>in vitro</em> models.</div></div><div><h3>Conclusion</h3><div>The review articles featured in the <em>Journal of Oral Biosciences</em> provide comprehensive insights into contemporary research themes and emerging concepts in oral biosciences. This editorial discusses their key findings and underscores their significance in advancing the understanding of oral health and disease.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"68 1","pages":"Article 100750"},"PeriodicalIF":2.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146229148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abaloparatide increases bone mass by increasing bone formation regardless of alendronate pretreatment in mice","authors":"Akito Makino ,&nbsp;Tomoka Hasegawa ,&nbsp;Tomomaya Yamamoto ,&nbsp;Hideko Takagi ,&nbsp;Takeshi Iijima ,&nbsp;Norio Amizuka","doi":"10.1016/j.job.2025.100708","DOIUrl":"10.1016/j.job.2025.100708","url":null,"abstract":"<div><div>The effects of bisphosphonate pretreatment on the bone anabolic effects of abaloparatide (ABL) in mice were investigated. Mice were administered alendronate (ALN) at different doses for 21 days, followed by 28 days of ABL. ABL increased femoral bone mineral density at all ALN doses, with effects equal to or greater than those observed in mice without ALN. Histological analysis demonstrated that ABL increased ALPase- and PHOSPHO1-positive osteoblasts, irrespective of ALN pretreatment. However, the increase in TRAP-positive osteoclasts by ABL was attenuated at higher ALN doses. These findings suggest that ABL promotes bone formation in mice, independently of ALN pretreatment.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"68 1","pages":"Article 100708"},"PeriodicalIF":2.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145580406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross-disease transcriptomic screening identifies fibroblast-associated mediators shared across periodontitis and rheumatoid arthritis","authors":"Yuta Izumi ,&nbsp;Taisuke Hani ,&nbsp;Tomoo Kudo ,&nbsp;Yukihiro Numabe ,&nbsp;Yuuichi Soeno","doi":"10.1016/j.job.2026.100751","DOIUrl":"10.1016/j.job.2026.100751","url":null,"abstract":"<div><h3>Objectives</h3><div>Periodontitis and rheumatoid arthritis (RA) are chronic inflammatory diseases characterized by progressive tissue destruction, and numerous studies have suggested that they are linked. However, the molecular mechanisms underlying this association remain unclear. The aim of this study was to identify molecular pathways beyond classical inflammation and immunity that are commonly dysregulated in periodontitis and RA.</div></div><div><h3>Methods</h3><div>We performed <em>in silico</em> screening of publicly available transcriptomic datasets for periodontitis and RA. Next, we performed experimental validation of candidate molecules in an <em>in vitro</em> culture model designed to mimic periodontal tissue under RA-associated conditions.</div></div><div><h3>Results</h3><div>Comparative analyses of healthy controls identified 540 differentially expressed genes (DEGs) in periodontitis, 4019 DEGs in RA, and 117 commonly upregulated DEGs. Among these, <em>CHI3L2</em> and <em>MMP3</em> were selected for further investigation and were found to be prominently expressed in gingival fibroblasts. Quantitative real-time polymerase chain reaction analyses showed that both genes were significantly upregulated by lipopolysaccharide stimulation and by conditioned medium derived from RA synovial cells, with a synergistic enhancement observed when both stimuli were applied simultaneously. Co-culture and wound-healing assays showed that RA synovial cells impaired proliferation and migration of gingival fibroblasts.</div></div><div><h3>Conclusions</h3><div>RA-associated pathological factors may influence the responsiveness of periodontal tissue to inflammatory stimuli. Although periodontitis and RA are primarily driven by immune-mediated inflammation, tissue-level molecular expression and sensitivity to stimulus may also be altered, providing complementary insights into shared disease mechanisms and their potential relevance for clinical management.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"68 1","pages":"Article 100751"},"PeriodicalIF":2.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Porphyromonas gingivalis-derived lipopolysaccharide on the barrier function of cultured junctional epithelial cells","authors":"Ryo Aizawa ,&nbsp;Masaru Sunaga ,&nbsp;Keisuke Tanaka ,&nbsp;Marika Sugano ,&nbsp;Daisuke Saito ,&nbsp;Koki Okada ,&nbsp;Junichi Tanaka ,&nbsp;Kenji Mishima ,&nbsp;Matsuo Yamamoto","doi":"10.1016/j.job.2025.100721","DOIUrl":"10.1016/j.job.2025.100721","url":null,"abstract":"<div><h3>Objectives</h3><div>The junctional epithelium (JE) plays an important role in maintaining the protective integrity of periodontal tissues by forming an epithelial barrier that impedes bacterial invasion. This study examined the effects of <em>Porphyromonas gingivalis</em> lipopolysaccharide (PG-LPS) on JE-1 cells, a mouse-derived junctional epithelial cell line.</div></div><div><h3>Methods</h3><div>JE-1 cells were exposed to PG-LPS, and the effects on cell viability, migration, gene expression, protein expression, and barrier function at various time points were evaluated according to the experimental type.</div></div><div><h3>Results</h3><div>PG-LPS (1 μg/mL) did not affect JE-1 cell viability but significantly inhibited migration. Treatment with PG-LPS upregulated inflammatory genes (<em>Il-6, Tlr2, Tlr4, Traf6</em>) and altered the expression of chemokines (increased <em>Cxcl2</em> and decreased <em>Cxcl10</em>) and protective factors (increased <em>Nfe2l2</em> and <em>Slpi</em>). Notably, 1 μg/mL of PG-LPS increased permeability and decreased adhesion molecule expression (<em>Cdh1</em> and <em>Itgb4</em>), whereas 10 μg/mL showed a non-linear response (apparent permeability [cm/s]: 0.868, 0.915, 1.416, 1.728, 1.224, and 1.176 with 0, 0.1, 0.5, 1, 5, and 10 μg/mL respectively), indicating compensatory mechanisms.</div></div><div><h3>Conclusions</h3><div>PG-LPS disrupts JE barrier function and alters its immunomodulatory properties, potentially contributing to periodontitis progression. These findings enhance our understanding of the underlying causes of periodontitis and inform targeted treatments to maintain JE defense against periodontopathogenic bacteria.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"68 1","pages":"Article 100721"},"PeriodicalIF":2.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145690960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone morphogenetic protein-2 and transforming growth factor-β1 regulate cell proliferation and ectopic claudin-4 expression in parotid acinar cells","authors":"Katsumasa Ueki,&nbsp;Megumi Yokoyama,&nbsp;Osamu Katsumata-Kato,&nbsp;Junko Fujita-Yoshigaki","doi":"10.1016/j.job.2026.100735","DOIUrl":"10.1016/j.job.2026.100735","url":null,"abstract":"<div><h3>Objectives</h3><div>Salivary gland secretory function is impaired by tissue injury, but subsequently recovers if the damage is not severe. We previously reported that tissue injury can cause secretory granule loss and ectopic expression of claudins and stem cell markers including nestin in parotid acinar cells. Such alterations may indicate acinar cell dedifferentiation contributing to cell survival. We investigated transforming growth factor (TGF)-β superfamily member expression profiles and functions to identify tissue injury responsive signals.</div></div><div><h3>Methods</h3><div>Acinar cells were isolated from the rat parotid glands via extracellular matrix digestion with collagenase and hyaluronidase and cultured in the absence or presence of the Src kinase inhibitor PP1. TGF-β superfamily expression was determined by reverse transcription quantitative PCR (RT-qPCR). Acinar cells were then cultured with inhibitors of bone morphogenetic protein (BMP) or TGF-β. A Cell Counting Kit-8 was used to assess cell proliferation, and immunoblotting was used to examine the expression of cell adhesion molecules and the stem cell marker nestin.</div></div><div><h3>Results</h3><div>Increased BMP-2, BMP-6, and TGF-β1 expression was observed in primary culture of parotid acinar cells. Their expression decreased under Src inhibitor treatment. BMP-2/4 and TGF-β signaling inhibitors suppressed cell proliferation. These inhibitors also decreased claudin-4 and nestin expression, which was absent in intact acinar tissue, but present after cell isolation.</div></div><div><h3>Conclusions</h3><div>BMP-2 and TGF-β1 may contribute to tissue regeneration and protection by enhancing paracellular barrier function.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"68 1","pages":"Article 100735"},"PeriodicalIF":2.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145976194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dipeptidyl peptidase from Streptococcus anginosus with substrate specificity for Xaa-Pro/Ala and potential impact on tumor immunity","authors":"Shu Suzuki ,&nbsp;Toshitaka Miura ,&nbsp;Yu Shimoyama ,&nbsp;Yuko Ohara-Nemoto ,&nbsp;Takayuki K. Nemoto ,&nbsp;Hiroyuki Yamada ,&nbsp;Taichi Ishikawa","doi":"10.1016/j.job.2025.100723","DOIUrl":"10.1016/j.job.2025.100723","url":null,"abstract":"<div><h3>Objectives</h3><div><em>Streptococcus anginosus</em>, an oral commensal bacterium, is a potential risk factor for malignancies of the oral cavity and upper gastrointestinal tract. Although this species harbors an Xaa-Pro dipeptidyl peptidase (DPP), its enzymatic characteristics and potential role in tumor-associated immune modulation remain unclear. In this study, <em>S. anginosus</em> Xaa-Pro DPP was characterized and its ability to cleave chemokine-related peptides was evaluated.</div></div><div><h3>Methods</h3><div>Six oral streptococcal strains were analyzed for DPP activity using fluorogenic dipeptidyl substrates. The gene encoding <em>S. anginosus</em> Xaa-Pro DPP was cloned and the recombinant enzyme was purified and characterized. The enzymatic activity of the peptidase against synthetic dipeptidyl 7-amino-4-methylcoumarin (MCA) and chemokine-derived peptides was assessed using fluorescence assays and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The effect of the DPP4 inhibitor, P32/98, was also examined.</div></div><div><h3>Results</h3><div><em>S. anginosus</em> had the highest DPP activity among the tested oral streptococci, particularly towards Gly-Pro- and Lys-Ala-MCA. The recombinant enzyme selectively removed Xaa-Pro and Xaa-Ala dipeptides and the N-terminal Lys-Pro dipeptide from the CXCL12-derived peptide, whereas incretins were minimally affected. The enzymatic activity of Xaa-Pro DPP against synthetic substrates was markedly inhibited by P32/98.</div></div><div><h3>Conclusion</h3><div><em>S. anginosus</em> Xaa-Pro DPP has specificity for N-terminal Xaa-Pro/Ala in peptides and exhibits the ability to inactivate chemokine-related peptides, suggesting a potential contribution to immune dysregulation in the tumor microenvironment.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"68 1","pages":"Article 100723"},"PeriodicalIF":2.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145747944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein behavior of the ligand of Numb-protein X 2 (LNX2) in mouse ameloblasts at the maturation stage","authors":"Takumi Sugimoto ,&nbsp;Miwako Matsuki-Fukushima ,&nbsp;Masakazu Nitta ,&nbsp;Takahiro Funatsu ,&nbsp;Naoko Nonaka","doi":"10.1016/j.job.2026.100752","DOIUrl":"10.1016/j.job.2026.100752","url":null,"abstract":"<div><h3>Objectives</h3><div>Enamel formation comprises two main stages: matrix formation (secretory), during which ameloblasts secrete an immature enamel matrix; and maturation, during which the matrix is degraded and remodeled. During maturation, the position of the cellular tight junctions change between the ruffle- and smooth-end phases; however, the regulatory mechanisms underlying this morphological alteration remain unclear. We focused on the Ligand of Numb-protein X (LNX): We would like to delete “ family of proteins” and add “PDZ-domain containig” to define the LNXs have ability to bind tight junctional proteins, which have been identified as PDZ-domain containing E3 ubiquitin ligases, and examined the changes in their expression and localization in ameloblasts.</div></div><div><h3>Methods</h3><div>Enamel organs were isolated from 5- and 13-day-old mouse molars, and rat incisors were sectioned at the secretory and maturation stages to evaluate mRNA expression. Double-immunofluorescence staining was performed using antibodies against LNX2 and other LNX2-related proteins in paraformaldehyde-fixed, decalcified mouse incisors.</div></div><div><h3>Results</h3><div>Among the two LNXs, LNX1 and LNX2, LNX2 had higher mRNA expression levels at the secretory and maturation stages, in the molars and in the incisors. Double immunofluorescence staining of the LNX-associated protein, Numb, an interaction partner of LNX2, was performed. LNX2 staining in ruffle-end ameloblasts during maturation was observed in two forms: (1) aggregated intracellular vesicles co-localized with Numb and (2) diffuse cytoplasmic labeling not co-localized with Numb. The lysosomal marker, lysosome-associated membrane protein-1, and claudin-7, a tight-junction membrane protein that predominantly localizes to the basolateral membrane, were present in the proximal and distal cytoplasmic regions, where LNX2 was also detected.</div></div><div><h3>Conclusions</h3><div>These results suggest that LNX2 contributes to the degradation of Numb and claudin-7 in ruffle-end ameloblasts during maturation.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"68 1","pages":"Article 100752"},"PeriodicalIF":2.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mouse strain and sex differences in the number and distribution of salivary gland IgA plasma cells","authors":"Midori Maekawa ,&nbsp;Yukinori Tanaka ,&nbsp;Kentaro Mizuta ,&nbsp;Shunji Sugawara","doi":"10.1016/j.job.2026.100755","DOIUrl":"10.1016/j.job.2026.100755","url":null,"abstract":"<div><h3>Objective</h3><div>Salivary immunoglobulin A (IgA) produced by IgA plasma cells (PCs) in the salivary glands (SGs) plays a key role in maintaining immune homeostasis in the oral mucosa. We investigated differences by sex and mouse strain in salivary IgA concentration and IgA PC distribution among the major SGs.</div></div><div><h3>Methods</h3><div>Pilocarpine-induced saliva was collected from male and female BALB/c and C57BL/6 mice, and salivary IgA concentrations were assessed using ELISA. Cells were isolated from the parotid, submandibular, and sublingual glands and analyzed using flow cytometry to quantify IgA PCs.</div></div><div><h3>Results</h3><div>Salivary IgA concentrations were higher in BALB/c mice than in C57BL/6 mice, with no sex-related differences. Total numbers of IgA PCs in the three major SGs were consistently higher in BALB/c mice than in C57BL/6 mice, with no sex differences. In both strains, male mice exhibited parotid gland-dominant distribution of IgA PCs, whereas female mice exhibited submandibular/sublingual gland-dominant distribution.</div></div><div><h3>Conclusions</h3><div>The present results reveal distinct strain and sex differences in salivary IgA concentrations and in numbers and distribution of SG IgA PCs.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"68 1","pages":"Article 100755"},"PeriodicalIF":2.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamin 2 is involved in osteoblast migration by regulating the organization of F-actin","authors":"Takumi Moriya ,&nbsp;A. Surong ,&nbsp;Nanami Tatsumi ,&nbsp;Hiroshi Yamada ,&nbsp;Fumiko Takemoto ,&nbsp;Hiroshi Kamioka ,&nbsp;Hirohiko Okamura ,&nbsp;Mika Ikegame","doi":"10.1016/j.job.2025.100720","DOIUrl":"10.1016/j.job.2025.100720","url":null,"abstract":"<div><h3>Objectives</h3><div>Dynamin, a GTPase that regulates membrane dynamics, has recently been implicated in actin cytoskeletal remodeling. This study aimed to elucidate the role of dynamin in osteoblast migration by examining the effects of dynamin inhibition on the localization and organization of F-actin and dynamin 2 in MC3T3-E1 cells.</div></div><div><h3>Methods</h3><div>MC3T3-E1 cells were treated with dynamin inhibitors (Dyngo 4a and Dynole 34-2), and cell migration was assessed using a wound-healing assay. Fluorescent staining was performed to analyze the intracellular localization of F-actin and dynamin 2.</div></div><div><h3>Results</h3><div>Dynamin inhibition significantly reduced the migration of MC3T3-E1 cells. Fluorescence analysis revealed a marked decrease in the accumulation and colocalization of F-actin and dynamin 2 at the protrusion edge. Additionally, dynamin inhibition suppressed the formation of lamellipodia and stress fibers while promoting the appearance of abnormal F-actin clusters in the cytoplasm.</div></div><div><h3>Conclusions</h3><div>These findings suggest that dynamin plays an essential role in osteoblast migration by regulating actin cytoskeletal remodeling, particularly through the formation of lamellipodia and stress fibers.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"68 1","pages":"Article 100720"},"PeriodicalIF":2.3,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145690962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}