Journal of Oral Biosciences最新文献

{"title":"Gene expression profiles in human dental pulp stem cells treated short-term with lipopolysaccharides before and after osteoinduction","authors":"Ai Orimoto ,&nbsp;Ziyi Wang ,&nbsp;Mitsuaki Ono ,&nbsp;Chiaki Kitamura ,&nbsp;Kentaro Ono","doi":"10.1016/j.job.2024.100603","DOIUrl":"10.1016/j.job.2024.100603","url":null,"abstract":"<div><h3>Objectives</h3><div>Dental pulp stem cells (DPSCs) are essential for reparative dentinogenesis following damage or infection. DPSCs surrounding theblood vessels in the central region of the dental pulp actively proliferate after tooth injury and differentiate into new odontoblast-like cells or odontoblasts to form reparative dentin. However, the signaling pathways involved in undifferentiated and osteodifferentiated DPSCs under inflammatory conditions remain unclear. This study aimed to compare the expression profiles of immortalized undifferentiated and osteo-differentiated human DPSCs (hDPSCs) treated with and without lipopolysaccharide (LPS) to elucidate the molecular regulatory mechanisms involved in inflammatory conditions.</div></div><div><h3>Methods</h3><div>We investigated the differences between undifferentiated and osteodifferentiated hDPSCs in response to LPS. RNA-seq analyses of undifferentiated and osteodifferentiated hDPSCs were performed with and without LPS.</div></div><div><h3>Results</h3><div>Whole-transcriptome profiling revealed distinct differences in the expression patterns of LPS-treated undifferentiated and osteodifferentiated DPSCs. Death-associated protein kinase 1 levels downregulated in LPS-treated osteodifferentiated cells, inhibiting apoptosis and enhancing cell survival After LPS treatment, osteodifferentiated DPSCs exhibited higher expression levels of various inflammatory cytokines and chemokines than undifferentiated DPSCs.</div></div><div><h3>Conclusion</h3><div>This study provides valuable transcriptomic data as a critical resource for uncovering potential therapeutic targets to enhance cell survival and regulate inflammation within the dental pulp. By elucidating the key molecular mechanisms and identifying specific gene expression changes linked to inflammatory and immune responses, these findings provide significant insights into osteo-differentiated hDPSCs.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100603"},"PeriodicalIF":2.6,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential role of chromodomain helicase DNA-binding protein 3 in defining the cervical width by regulating the early growth stage of the apical papilla during tooth development","authors":"Kento Shimamura ,&nbsp;Toshiki Nojiri ,&nbsp;Hisatomo Kondo ,&nbsp;Yunosuke Ikeda ,&nbsp;Rika Yasuhara ,&nbsp;Hiroko Ida-Yonemochi ,&nbsp;Keishi Otsu ,&nbsp;Hidemitsu Harada ,&nbsp;Kenji Mishima ,&nbsp;Hayato Ohshima ,&nbsp;Takuya Kobayashi ,&nbsp;Tarou Irié","doi":"10.1016/j.job.2024.100604","DOIUrl":"10.1016/j.job.2024.100604","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to evaluate the role of the chromodomain helicase DNA-binding protein 3 (CHD3) in tooth morphogenesis in <em>Chd3</em> knockout mice.</div></div><div><h3>Methods</h3><div><em>Chd3</em> knockout mice were generated using the CRISPR-Cas9 method. Mandibular first molars were extracted from the mice and their littermates and morphometrically analyzed. Subsequent histological and immunohistochemical analyses of teeth were performed at each developmental stage. <em>Chd3</em> knockdown in mesenchymal cells from the dental papilla (mDP) and Hertwig's epithelial root sheath (HERS) was performed by <em>Chd3</em> shRNA transduction or a control using an adenoviral vector. These effects were examined using cell proliferation assays and quantitative real-time polymerase chain reaction.</div></div><div><h3>Results</h3><div>Narrowing of tooth cervical width was observed in mandibular first molars of <em>Chd3</em> knockout mice. On postnatal day (PN) 8, the cervical width was narrow before root formation in tooth germs. The number of Ki-67-positive cells decreased in the dental mesenchyme at PN1 and apical papilla at PN8. <em>Chd3</em> promoted the proliferation of dental mesenchymal cells, but no significant changes were observed in HERS epithelial cells. <em>Chd3</em> maintained sonic hedgehog (<em>Shh</em>) expression and inhibited that of bone morphogenetic protein (<em>Bmp</em>)<em>4</em> in dental mesenchymal cells, maintaining <em>Shh</em> and <em>Wnt3a</em> expression and inhibited that of <em>Bmp2</em> in HERS epithelial cells.</div></div><div><h3>Conclusion</h3><div><em>Chd3</em> may regulate tooth cervical width during the early growth stage of the apical papilla via <em>Shh</em>, <em>Bmp,</em> and <em>Wnt</em> signaling.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100604"},"PeriodicalIF":2.6,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-molecular-weight polyphenol promotes cell sensitivity to cisplatin and alleviates cancer-related muscle atrophy via NF-κB suppression in oral squamous cell carcinoma","authors":"Yun-Ching Chang ,&nbsp;Yu-Yan Lan ,&nbsp;Hung-Yu Lin ,&nbsp;Cheng Liu ,&nbsp;Sue-Joan Chang","doi":"10.1016/j.job.2024.100595","DOIUrl":"10.1016/j.job.2024.100595","url":null,"abstract":"<div><h3>Objective</h3><div>Drug resistance and subsequent adverse effects, such as cancer cachexia, limit the clinical use of cisplatin. Oligonol® (Olg), a low-molecular-weight polyphenol, exhibits NF-κB inhibitory properties. NF-κB activation has been implicated in cisplatin resistance of cancer cells and skeletal muscle wasting. Therefore, we hypothesized that combined cisplatin and Olg could overcome chemoresistance and reduce muscle atrophy.</div></div><div><h3>Methods</h3><div>To investigate the efficiency of Olg, oral squamous cell carcinoma (OSCC) cells were used for chemosensitivity, and human skeletal muscle myoblast (HSkMC) was used for muscle atrophy. HSkMCs treated with OSCC cell-derived conditioned medium were used to examine the role of Olg in muscle atrophy mediated by the tumor inflammatory microenvironment.</div></div><div><h3>Results</h3><div>Olg exerted little effect on the viability of OSCC cells by promoting apoptotic cell death. However, it exhibited excellent capability to enhance the sensitivity of OSCC cells to cisplatin and overcome the acquired cisplatin resistance of OSCC. We revealed that NF-κB signaling contributes to cisplatin resistance in OSCC cells, whereas Olg enhances cell sensitivity to cisplatin by NF-κB suppression. Conversely, Olg contributes to a positive protein turnover and alleviates cisplatin-induced muscle atrophy by regulating Akt/mTOR/p70S6K and NF-κB/MuRF1 pathway. Olg represses TNF-α and interleukin 6 driven from OSCC cells and alleviates muscle atrophy mediated by the tumor inflammatory microenvironment.</div></div><div><h3>Conclusions</h3><div>Olg enhanced cisplatin chemosensitivity and reduced its adverse effects on skeletal muscle, suggesting its potential as a chemosensitizing agent for cisplatin. Further animal and clinical studies are required to validate these findings.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100595"},"PeriodicalIF":2.6,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histochemical assessment of the femora of spontaneously diabetic torii-leprfa (SDT-fa/fa) rats that mimic type II diabetes","authors":"Qi Yao ,&nbsp;Kanako Tsuboi ,&nbsp;Hiromi Hongo ,&nbsp;Mako Sakakibara ,&nbsp;Tomomaya Yamamoto ,&nbsp;Mai Haraguchi-Kitakamae ,&nbsp;Hotaka Ishizu ,&nbsp;Xuanyu Liu ,&nbsp;Yan Shi ,&nbsp;Weisong Li ,&nbsp;Jiaxin Cui ,&nbsp;Tomohiro Shimizu ,&nbsp;Norio Amizuka ,&nbsp;Atsuro Yokoyama ,&nbsp;Tomoka Hasegawa ,&nbsp;Kiwamu Sakaguchi","doi":"10.1016/j.job.2024.100602","DOIUrl":"10.1016/j.job.2024.100602","url":null,"abstract":"<div><h3>Objective</h3><div>To elucidate the mechanisms underlying diabetic osteoporosis, we conducted a comprehensive histological examination of the femora of Spontaneously Diabetic Torii-<em>Lepr</em>^<em>fa</em> (SDT-<em>fa/fa</em>) rats, an established model of obesity-related type 2 diabetes.</div></div><div><h3>Materials and methods</h3><div>Femora from 12 30-week-old male SDT-<em>fa/fa</em> rats and age-matched Sprague–Dawley (SD) rats (controls) were used for detailed histochemical analyses, including tartrate-resistant acid phosphatase (TRAP), cathepsin K, tissue nonspecific alkaline phosphatase (ALP), phosphoethanolamine/phosphocholine phosphatase 1 (PHOSPHO1), dentin matrix protein 1 (DMP-1), matrix extracellular phosphoglycoprotein (MEPE), sclerostin, osteocalcin staining, silver impregnation, von Kossa staining, and micro-computed tomography (CT).</div></div><div><h3>Results</h3><div>Micro-CT and hematoxylin-eosin staining demonstrated significantly reduced trabecular bone volume in the femoral metaphyses of SDT-<em>fa/fa</em> rats. Although the number of TRAP-positive osteoclasts per bone surface remained comparable between both groups, SDT-<em>fa/fa</em> rats exhibited reduced areas of ALP-positive and PHOSPHO1-reactive mature osteoblasts/BS. Silver impregnation revealed a well-organized osteocytic lacunar–canalicular system in both groups. However, immunostaining identified aberrant DMP-1 and MEPE expression localized predominantly in the lacunae in SDT-<em>fa/fa</em> rats and in the lacunae and canaliculi of SD rats. Additionally, intense osteocalcin and sclerostin immunoreactivity was detected in osteocytes, along with a higher proportion of osteocalcin-positive osteocytes in SDT-<em>fa/fa</em> rats, distinguishing them from controls.</div></div><div><h3>Conclusion</h3><div>SDT-<em>fa/fa</em> rats displayed a significant decline in osteoblastic function and distinctive distribution patterns of osteocyte-derived peptides, suggesting that this diabetic model may manifest alterations in osteoblastic activity and the osteocytic lacunar-canalicular network.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100602"},"PeriodicalIF":2.6,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of anterior disc displacement and estrogen deficiency on rabbit mandibular condyle","authors":"Takuya Iwasaki ,&nbsp;Namiaki Takahara ,&nbsp;Vu Viet Duc ,&nbsp;Nobuyoshi Tomomatsu ,&nbsp;Makoto J. Tabata ,&nbsp;Tetsuya Yoda","doi":"10.1016/j.job.2024.100599","DOIUrl":"10.1016/j.job.2024.100599","url":null,"abstract":"<div><h3>Objective</h3><div>In this study, we aimed to establish an experimental model of idiopathic condylar resorption by combining surgically induced anterior disc displacement and estrogen deficiency in growing rabbits. This study aimed to investigate the individual and combined effects of these factors on condylar resorption.</div></div><div><h3>Materials and methods</h3><div>Seventeen female Japanese White rabbits were divided into four groups: control, ovariectomy, anterior disc displacement surgery, and combination of ovariectomy and anterior disc displacement surgery. Micro-computed tomography and histological evaluations were performed to analyze changes in the trabecular bone structure and cartilage thickness of the condyle.</div></div><div><h3>Results</h3><div>The combined group exhibited the most significant changes in trabecular bone parameters, including the lowest bone volume per tissue volume and trabecular number and the highest trabecular separation and spacing. Histologically, the fibrous layer thinned and the hypertrophic layer thickened in the anterior and central parts of the condyle in the anterior disc displacement and combined groups. The Modified Mankin Score indicated the highest level of degenerative change in the combined group.</div></div><div><h3>Conclusion</h3><div>The combination of surgically induced anterior disc displacement and estrogen deficiency in growing rabbits effectively modeled idiopathic condylar resorption, demonstrating the synergistic impact of disc displacement and estrogen deficiency on condylar resorption. This model provides valuable insight into the pathogenesis of idiopathic condylar resorption.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100599"},"PeriodicalIF":2.6,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142839994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Measurement of advanced glycation end products (AGEs) accumulated in dentin collagen","authors":"Ruri Asami,&nbsp;Takuya Sato,&nbsp;Koji Sakiyama","doi":"10.1016/j.job.2024.100600","DOIUrl":"10.1016/j.job.2024.100600","url":null,"abstract":"<div><h3>Objective</h3><div>In the Maillard reaction, reducing sugars bind to proteins in a non-enzymatic manner, and the final products are collectively named, advanced glycation end products (AGEs). AGEs accumulate in the human body with age and cause various disorders in tissues and cells. In this study, pentosidine, an AGE that can be stably measured, was used to investigate the differences in pentosidine accumulation within dentin, and whether the results can indicate age.</div></div><div><h3>Methods</h3><div>High-performance liquid chromatography was used to measure pentosidine, an AGE that accumulates in dentin collagen, in extracted teeth with no caries and a clear history.</div></div><div><h3>Results</h3><div>The dentin pentosidine concentration increased with age, with a higher pentosidine concentration observed at the enamel-dentin junction aspect than at the pulp cavity aspect. A close correlation was observed between the dentin pentosidine level and age.</div></div><div><h3>Conclusions</h3><div>These findings suggest the utility of measuring accumulation of AGEs in dentin collagen as an indicator of age.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100600"},"PeriodicalIF":2.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential anti-inflammatory effect of hyaluronic acid gel alone or in combination with grapefruit seed extract on induced periodontitis in mandibular molars of Wistar rats","authors":"Sana Mostafa ,&nbsp;Aiah A. El-Rashidy ,&nbsp;Mohamed Talaat Elbehwashy ,&nbsp;Manar A. Abdul-Aziz ,&nbsp;Nermeen AbuBakr","doi":"10.1016/j.job.2024.100598","DOIUrl":"10.1016/j.job.2024.100598","url":null,"abstract":"<div><h3>Objectives</h3><div>Antimicrobial agents have been used in conjunction with conventional chemomechanical therapy to improve the treatment outcomes of periodontitis. This study aimed to evaluate the ameliorating effect of topical application of hyaluronic acid (HA) with or without grapefruit seed extract (GFSE) (5, 10, and 15 wt %) in induced periodontitis in rats.</div></div><div><h3>Methods</h3><div>Surgical alveolar bone defects were created in 30 adult male Wistar rats, followed by the introduction of a ligature impregnated with Escherichia coli lipopolysaccharide for four weeks to induce periodontitis. Rats were distributed into five groups (n = 6); an untreated periodontitis group and four treated groups in which gel (HA±GFSE) was injected into the sulcus once weekly for two weeks. All rats were euthanized six weeks after starting the experiment, and the mandibles were prepared for histopathological and histomorphometric analyses. Enzyme-linked immunosorbent assay was used for measuring tissue levels of tumor necrosis factor-alpha (TNF-α), transforming growth factor-β1 (TGF-β1), and paraoxonase-1 (PON-1) enzyme.</div></div><div><h3>Results</h3><div>HA enhanced new bone formation at defect margins, thereby diminishing defect width. This effect significantly increased as HA was combined with GFSE in a dose-dependent manner. Moreover, the HA and GFSE mixture suppressed the tissue levels of TNF-α and TGF-β1, thereby increasing PON-1.</div></div><div><h3>Conclusion</h3><div>The HA and GFSE mixtures exhibited synergistic therapeutic potential for the treatment of chronic periodontitis in a dose-dependent manner.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100598"},"PeriodicalIF":2.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of trigeminal ganglion satellite glial cells in masseter muscle pain hypersensitivity","authors":"Sho Sawada ,&nbsp;Suzuro Hitomi ,&nbsp;Yoshinori Hayashi ,&nbsp;Hirotaka Shinozuka ,&nbsp;Yoshiyuki Yonehara ,&nbsp;Koichi Iwata ,&nbsp;Masamichi Shinoda","doi":"10.1016/j.job.2024.100596","DOIUrl":"10.1016/j.job.2024.100596","url":null,"abstract":"<div><h3>Objectives</h3><div>The underlying mechanism of masseter muscle pain hypersensitivity by sustained masseter muscle contraction (SMMC) is not well understood. This study aimed to examine whether the activation of satellite glial cells in the trigeminal ganglion (TG) contributes to masseter muscle pain hypersensitivity induced by SMMC.</div></div><div><h3>Methods</h3><div>Electrodes were placed on the masseter muscle fascia of rats to induce strong contractions, by daily electrical stimulation. Pain sensitivity in the masseter muscle was measured and the activation level of satellite glial cells in the TG was examined. The localization of P2Y₁₂ and the effects of P2Y₁₂ receptor inhibition on SMMC-induced pain hypersensitivity were evaluated. The amount of tumor necrosis factor alpha (TNF-α) and TNF-α receptor localization were determined in the TG.</div></div><div><h3>Results</h3><div>SMMC induced masseter muscle pain hypersensitivity and activation of satellite glial cells. P2Y₁₂ receptors were expressed in satellite glial cells and masseter muscle pain hypersensitivity was suppressed by intra-TG P2Y₁₂ receptor antagonism. TG neurons innervating the sustained-contracted masseter muscle expressed TNF-α receptor and SMMC increased TNF-α levels in TG.</div></div><div><h3>Conclusion</h3><div>SMMC-induced activation of satellite glial cells though the P2Y₁₂ receptor signaling may contribute to masseter muscle pain hypersensitivity via the TNF-α signaling pathway.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100596"},"PeriodicalIF":2.6,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The cortical areas processing periodontal ligament nociception in mice","authors":"Risako Okuma ,&nbsp;Shutaro Kobayashi ,&nbsp;Satomi Kobayashi ,&nbsp;Yoshinori Arai ,&nbsp;Naoyuki Matsumoto ,&nbsp;Mitsuru Motoyoshi ,&nbsp;Masayuki Kobayashi ,&nbsp;Satoshi Fujita","doi":"10.1016/j.job.2024.100597","DOIUrl":"10.1016/j.job.2024.100597","url":null,"abstract":"<div><h3>Objectives</h3><div>Toothaches are often poorly localized. Although periodontal pain is better localized, it can spread to other areas. Ultimately, the cerebral cortex processes nociception, with somatotopic organization possibly playing a role in localizing the origin. However, the exact cortical area in the periodontal ligament (PDL) remains unclear.</div></div><div><h3>Methods</h3><div>This study examined cortical responses to electrical stimulation of the molar PDL in anesthetized male mice using <em>in vivo</em> optical imaging with a voltage-sensitive dye, autofluorescent flavin fluorescence, and immunohistochemistry for c-Fos protein expression.</div></div><div><h3>Results</h3><div>On optical imaging, cortical responses to the stimulation of the ipsilateral and contralateral PDL of the upper and lower teeth were observed in the primary somatosensory cortex (S1) and area from the insular cortex (IC) to the ventral edge of the secondary somatosensory cortex (S2), defined as the area caudal to the middle cerebral artery (C-area). Responses in S1 were faint and unstable, but were consistent in the C-area. The initial response locations were similar regardless of which PDL was stimulated, and the activated areas in the C-area almost overlapped. Three-dimensional construction of c-Fos-immunopositive cells responding to upper or lower PDL stimulation revealed bilateral distribution in the cingulate gyrus, secondary auditory cortex, temporal association cortex, ectorhinal cortex, and IC, but not in the S1 and S2.</div></div><div><h3>Conclusion</h3><div>These results suggest that the somatotopic organization of the S1, S2, and IC cannot explain the localization of PDL nociception. The predominance of responses in the contralateral IC may provide clues for identifying the laterality.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100597"},"PeriodicalIF":2.6,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142819828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mirror-polished ceria-stabilized zirconia/alumina nanocomposite enhances gingival junctional epithelial cell adhesion","authors":"Shoma Yamamori ,&nbsp;Eri Urano-Morisawa ,&nbsp;Ayako Mochizuki ,&nbsp;Ryo Aizawa ,&nbsp;Fuminori Iwasa ,&nbsp;Matsuo Yamamoto ,&nbsp;Kazuyoshi Baba","doi":"10.1016/j.job.2024.100593","DOIUrl":"10.1016/j.job.2024.100593","url":null,"abstract":"<div><h3>Objectives</h3><div>The aim of this study was to determine the optimal surface roughness (Ra) of ceria-stabilized zirconia/alumina nanocomposite (Ce-TZP/Al₂O₃) implants for mouse gingival junctional epithelial cell (JE-1) adhesion and soft tissue sealing <em>in vitro</em>.</div></div><div><h3>Methods</h3><div>Titanium and Ce-TZP/Al₂O₃ disks were prepared, mechanically polished (M), and mirror-polished (Mr). The surface morphology of each disk was evaluated, and the Ra was measured using scanning electron microscopy and atomic force microscopy. JE-1 cells were cultured on each disk, and cell proliferation was assessed by measuring the absorbance using the MTS assay. We also analyzed the expression of the adhesion proteins Laminin-5, Integrin β4, and Cadherin-1 using immunostaining and quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The adhesion strength of the JE-1 cells to each disk was measured using a shaking stimulation test.</div></div><div><h3>Results</h3><div>M disks had rough surfaces, whereas Mr disks had smooth morphologies. JE-1 cell proliferation was proportional to the culture time, and the Mr disks showed higher values than the M disk. Immunofluorescence and qRT-PCR showed that expression of Laminin-5 and Integrin β4 was higher with Mr disks than with M on the Ce-TZP/Al₂O₃ disks. The oscillatory stimulation test also showed that the adhesive strength of JE-1 cells was significantly higher with Mr than with M on the Ce-TZP/Al₂O₃ disks.</div></div><div><h3>Conclusions</h3><div>Mirror polishing of Ce-TZP/Al₂O₃ disks enhances epithelial cell proliferation and adhesion more than mechanical polishing. These findings have implications for the optimization of implant surface characteristics to improve epithelial sealing.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100593"},"PeriodicalIF":2.6,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}