Journal of Oral Biosciences最新文献

{"title":"Specific localization of fibroblasts at the intercalated duct in the major salivary glands of rats","authors":"Go Onozawa ,&nbsp;Arata Nagasaka ,&nbsp;Yasuhiko Bando ,&nbsp;Koji Sakiyama ,&nbsp;Nobuharu Yamamoto ,&nbsp;Osamu Amano","doi":"10.1016/j.job.2024.02.004","DOIUrl":"10.1016/j.job.2024.02.004","url":null,"abstract":"<div><h3>Objectives</h3><p>Immunohistochemical methods were employed to investigate the morphological heterogeneity and localization of fibroblasts associated with the function of major salivary glands in rats.</p></div><div><h3>Methods</h3><p>Histochemical and electron microscopic observations were made in rat parotid, submandibular, and sublingual glands and pancreas. Fibroblasts were immunostained using their specific marker, 47 kDa heat shock protein (Hsp47).</p></div><div><h3>Results</h3><p>Hsp47-immunopositive fibroblasts within the intralobular connective tissue exhibited a notably smaller size compared with the interlobular connective tissue. They were loosely distributed throughout the connective tissue. However, fibroblasts with elongated long processes were explicitly identified at the intercalated ducts in parotid, sublingual, and submandibular glands. Fibroblastic bodies and processes were tightly approximated with the basement membrane of the duct. Electron microscopy confirmed these findings, revealing a thin layer consisting of collagen fibers was found between the fibroblasts and the basement membrane. Double staining of Hsp47 and α-smooth muscle actin (αSMA) in parotid glands indicating that Hsp47-positive fibroblasts enveloped both the duct and αSMA-positive myoepithelial cells. Additionally, They projected long and thin processes longitudinally at the straight portion or circularly at the bifurcated portion of the duct. The three-dimensional reconstruction showed a frame-like structure of fibroblasts surrounding the intercalated duct with longitudinal myoepithelial cells. However, such specific localization of fibroblasts was not detected in the exocrine pancreas lacking myoepithelium.</p></div><div><h3>Conclusions</h3><p>Small fibroblasts with long processes connecting or overwrapping each other and thin collagen layers surround the intercalated ducts in rat major salivary glands, presumably contributing to protecting the ducts from salivary flow and myoepithelial contraction.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 2","pages":"Pages 456-464"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139933484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abnormal bone regeneration induced by FK506 in medaka fin revealed by in vivo imaging","authors":"Kai Otake ,&nbsp;Yuki Azetsu ,&nbsp;Masahiro Chatani ,&nbsp;Akiko Karakawa ,&nbsp;Satoko Nishida ,&nbsp;Aiko Hirayama ,&nbsp;Rina Kobayashi ,&nbsp;Nobuhiro Sakai ,&nbsp;Noriyuki Suzuki ,&nbsp;Masamichi Takami","doi":"10.1016/j.job.2024.02.007","DOIUrl":"10.1016/j.job.2024.02.007","url":null,"abstract":"<div><h3>Objectives</h3><p>Bone tissue in bony fish demonstrates a remarkable ability to regenerate, particularly evident following induction of extensive bone defects, such as fin amputation. This regenerative capacity has been reported to be promoted by the immunosuppressant FK506, yet its precise effects on bone cells during fin regeneration remains insufficiently elucidated. This study aims to investigate the effects of FK506 treatment on bone morphology, osteoblasts, and osteoclasts in the bony fin rays of <em>osterix</em> promoter-DsRed/<em>TRAP</em> promoter-EGFP double transgenic (Tg) medaka.</p></div><div><h3>Methods</h3><p>The caudal fin of double Tg medaka was amputated, followed by a 20-day treatment with FK506 (1.0 μg/ml) to observe its effects on fin regeneration. Additionally, the regenerated caudal fin area underwent evaluation using genetic analysis and cell proliferation assays.</p></div><div><h3>Results</h3><p>FK506 treatment significantly increased <em>osterix</em>-positive osteoblast formation, resulting in both a significantly longer fin length and fewer joints in the bony fin rays formed during fin regeneration. Notably, <em>TRAP</em>-positive osteoclast formation and bone resorption were observed to occur primarily during the latter stages of fin regeneration. Furthermore, while the expression levels of osteoblast-related genes in the regenerated area remained unchanged following FK506 treatment, a heightened cell proliferation was observed at the tip of the fin.</p></div><div><h3>Conclusions</h3><p>Our findings suggest that treatment with FK506 promotes bone regeneration by increasing the number of osteoblasts in the amputated area of the fin. However, long-term treatment disrupts regular bone metabolism by inducing abnormal osteoclast formation.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 2","pages":"Pages 381-390"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139997820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-cancer agent piperlongumine is an inhibitor of transient receptor potential melastatin 7 channel in oral squamous cell carcinoma","authors":"Tao Su ,&nbsp;Yi-hui Chen ,&nbsp;Kan-kui Wu ,&nbsp;Xiao-hong Xu","doi":"10.1016/j.job.2024.03.002","DOIUrl":"10.1016/j.job.2024.03.002","url":null,"abstract":"<div><h3>Objectives</h3><p>To elucidate the association between the anticancer activities of piperlongumine (PL) and its potential target, transient receptor potential melastatin 7 channel (TRPM7), in oral squamous cell carcinoma (OSCC).</p></div><div><h3>Methods</h3><p>The expression levels and electrical characteristics of TRPM7 as well as cell viability in response to various PL treatments were investigated in the OSCC cell line Cal27.</p></div><div><h3>Results</h3><p>PL treatment resulted in a concentration- and time-dependent reduction in <em>TRPM7</em> mRNA and protein expression in Cal27 cells. Furthermore, PL treatment inhibited TRPM7-like rectifying currents in Cal27 cells; however, this inhibition was less effective than that of the TRPM7 antagonist waixenicin A. Rapid perfusion and washout experiments revealed an immediate inhibitory effect of PL on TRPM7-like currents. The antagonistic effect of PL occurred within 1 min and was not completely reversed following washout. Notably, the extracellular Ca²⁺ concentration still influenced PL-induced changes in the TRPM7-like current, indicating that PL can directly but gently antagonize the TRPM7 channel. Functional changes in TRPM7 correlated with the observed antiproliferative and cytotoxic effects of PL in Cal27 cells.</p></div><div><h3>Conclusions</h3><p>These findings suggest that PL exhibits potent inhibitory effects on TRPM7 and exerts its anti-cancer effects by downregulating TRPM7 expression and antagonizing channel currents.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 2","pages":"Pages 430-438"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140060723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional roles of descending projections from the cerebral cortex to the trigeminal spinal subnucleus caudalis in orofacial nociceptive information processing","authors":"Masayuki Kobayashi ,&nbsp;Yuka Nakaya ,&nbsp;Satomi Kobayashi","doi":"10.1016/j.job.2024.05.004","DOIUrl":"10.1016/j.job.2024.05.004","url":null,"abstract":"<div><h3>Background</h3><p>The trigeminal spinal subnucleus caudalis (Sp5C), also known as the medullary dorsal horn, receives orofacial somatosensory inputs, particularly nociceptive inputs, from the trigeminal nerve. In the Sp5C, excitatory and inhibitory neurons, glutamatergic and GABAergic/glycinergic neurons, respectively, form the local circuits. The axons of the glutamatergic neurons in lamina I ascend toward the thalamic and parabrachial nuclei, and this projection is the main pathway of orofacial nociception. Additionally, the axons of the higher brain regions, including the locus coeruleus, dorsal raphe, and cerebral cortex, are sent to the Sp5C.</p></div><div><h3>Highlight</h3><p>Among these descending projections, this review focuses on the functional profiles of the corticotrigeminal projections to the Sp5C, along with their anatomical aspects. The primary and secondary somatosensory and insular cortices are of particular interest.</p></div><div><h3>Conclusion</h3><p>Corticotrigeminal projections from the somatosensory cortex to the Sp5C play a suppressive role in nociceptive information processing, whereas recent studies have demonstrated a facilitative role of the insular cortex in nociceptive information processing at the Sp5C level.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 2","pages":"Pages 304-307"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140909455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor microenvironment of ameloblastoma with a focus on osteoclastogenesis, cell migration, and malignant transformation","authors":"Shohei Yoshimoto ,&nbsp;Kazuhiko Okamura","doi":"10.1016/j.job.2024.05.002","DOIUrl":"10.1016/j.job.2024.05.002","url":null,"abstract":"<div><h3>Background</h3><p>Odontogenic tumors arise in the jawbone and originate from cells associated with tooth development. Therefore, understanding odontogenic tumors requires knowledge of all aspects of dental research, including tooth development and eruption. Ameloblastoma is the most common odontogenic tumor.</p></div><div><h3>Highlight</h3><p>Although a benign tumor, ameloblastoma progresses with marked jawbone resorption. Because of its locally aggressive features, it can be treated surgically by resecting the surrounding bone. From a molecular pathology perspective, several genetic mutations and dysregulated signaling pathways involved in ameloblastoma tumorigenesis have been identified. Histopathologically, ameloblastomas consist of peripheral ameloblast-like cells and an inner stellate reticulum. The stromal region consists of fibrovascular connective tissue, showing a characteristic sparse myxoid histology. In general, the tumor microenvironment, including the surrounding non-tumor cells, contributes to tumorigenesis and progression. In this review, we focus on the tumor microenvironment of ameloblastomas. In addition, we present some of our recent studies on osteoclastogenesis, tubulin acetylation-induced cell migration, and hypoxia-induced epithelial–mesenchymal transition in ameloblastomas.</p></div><div><h3>Conclusion</h3><p>Further research on ameloblastomas can lead to the development of new treatments and improve patients’ quality of life.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 2","pages":"Pages 314-319"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140909462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of tooth loss on behavioral and psychological symptoms of dementia in app knock-in mice","authors":"Masae Furukawa ,&nbsp;Hirobumi Tada ,&nbsp;Resmi Raju ,&nbsp;Jingshu Wang ,&nbsp;Haruna Yokoi ,&nbsp;Mitsuyoshi Yamada ,&nbsp;Yosuke Shikama ,&nbsp;Takashi Saito ,&nbsp;Takaomi C. Saido ,&nbsp;Kenji Matsushita","doi":"10.1016/j.job.2024.03.005","DOIUrl":"10.1016/j.job.2024.03.005","url":null,"abstract":"<div><h3>Objectives</h3><p>Many patients with Alzheimer's disease (AD) experience behavioral and psychological symptoms of dementia (BPSD), which significantly affect their quality of life. It is known that 5-Hydroxytryptamine (5-HT) plays a crucial role in the development of BPSD. While the relationship between tooth loss and AD symptoms has been acknowledged, the aspect of aggression has not been focused on until now. Despite the established importance of 5-HT in BPSD, how tooth loss is related to the exacerbation of AD symptoms, especially in terms of aggression, remains largely unexplored. Although nutritional status is known to influence the progression of dementia, the specific effect of tooth loss on peripheral symptoms, notably aggression, is not well understood.</p></div><div><h3>Methods</h3><p>In our study, we conducted maxillary molar extractions in aged C57BL/6J and <em>App</em>^{<em>NL-G-F</em>} mice and observed their condition over a 3-month period. During this time, we documented significant behavioral and genetic differences between mice in the control groups and mice that underwent tooth extraction. Notably, mice that underwent tooth extraction exhibited a considerable decline in cognitive function and increased in aggression 3 months after tooth extraction compared with the control groups (C57BL/6J and <em>App</em>^{<em>NL-G-F</em>}mice).</p></div><div><h3>Results</h3><p>Our findings suggest that molar loss may lead to reduced 5-HT levels in the hippocampus, possibly mediated by the trigeminal nerve, contributing to the development of aggression and BPSD in AD.</p></div><div><h3>Conclusion</h3><p>This study sheds light on the intricate relationships between oral health, 5-HT, and AD symptoms, offering valuable insights into potential therapeutic avenues for managing BPSD in patients with dementia.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 2","pages":"Pages 329-338"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1349007924000744/pdfft?md5=5ddb384ef8a89c918dfd65de96394830&pid=1-s2.0-S1349007924000744-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140194811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transposon insertion in Rothia dentocariosa","authors":"Boang Liu ,&nbsp;Chiho Mashimo ,&nbsp;Takayuki Nambu ,&nbsp;Hugo Maruyama ,&nbsp;Toshinori Okinaga","doi":"10.1016/j.job.2024.04.006","DOIUrl":"10.1016/j.job.2024.04.006","url":null,"abstract":"<div><h3>Objectives</h3><p><em>Rothia</em> spp. are emerging as significant bacteria associated with oral health, with <em>Rothia dentocariosa</em> being one of the most prevalent species. However, there is a lack of studies examining these properties at the genetic level. This study aimed to establish a genetic modification platform for <em>R. dentocariosa.</em></p></div><div><h3>Methods</h3><p><em>Rothia</em> spp. were isolated from saliva samples collected from healthy volunteers. Subsequently, <em>R. dentocariosa</em> strains were identified through colony morphology, species-specific polymerase chain reaction (PCR), and 16S ribosomal RNA gene sequencing. The identified strains were then transformed with plasmid pJRD215, and the most efficient strain was selected. Transposon insertion mutagenesis was performed to investigate the possibility of genetic modifications.</p></div><div><h3>Results</h3><p>A strain demonstrating high transforming ability, designated as <em>R. dentocariosa</em> LX16, was identified. This strain underwent transposon insertion mutagenesis and was screened for 5-fluoroorotic acid-resistant transposants. The insertion sites were confirmed using arbitrary primed PCR, gene-specific PCR, and Sanger sequencing.</p></div><div><h3>Conclusion</h3><p>This study marks the first successful genetic modification of <em>R. dentocariosa</em>. Investigating <em>R. dentocariosa</em> at the genetic level can provide insights into its role within the oral microbiome.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 2","pages":"Pages 358-364"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140785624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Title Page (without cities)","authors":"","doi":"10.1016/S1349-0079(24)00096-3","DOIUrl":"https://doi.org/10.1016/S1349-0079(24)00096-3","url":null,"abstract":"","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 2","pages":"Page i"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1349007924000963/pdfft?md5=9622197237dbe4c88c186c497a4739c7&pid=1-s2.0-S1349007924000963-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141308428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osteoblast differentiation of Gli1⁺ cells via Wnt and BMP signaling pathways during orthodontic tooth movement","authors":"Yuri Seki ,&nbsp;Hiroaki Takebe ,&nbsp;Yuya Nakao ,&nbsp;Kohei Sato ,&nbsp;Toshihide Mizoguchi ,&nbsp;Hiroaki Nakamura ,&nbsp;Masahiro Iijima ,&nbsp;Akihiro Hosoya","doi":"10.1016/j.job.2024.03.004","DOIUrl":"10.1016/j.job.2024.03.004","url":null,"abstract":"<div><h3>Objectives</h3><p>Factors that induce bone formation during orthodontic tooth movement (OTM) remain unclear. Gli1 was recently identified as a stem cell marker in the periodontal ligament (PDL). Therefore, we evaluated the mechanism of differentiation of Cre/LoxP-mediated Gli1/Tomato⁺ cells into osteoblasts during OTM.</p></div><div><h3>Methods</h3><p>After the final administration of tamoxifen to 8-week-old Gli1-Cre^ERT2/ROSA26-loxP-stop-loxP-tdTomato mice for 2 days, nickel–titanium closed coil springs were attached between the upper anterior alveolar bone and the first molar. Immunohistochemical localizations of β-catenin, Smad4, and Runx2 were observed in the PDL on 2, 5, and 10 days after OTM initiation.</p></div><div><h3>Results</h3><p>In the untreated tooth, few Gli1/Tomato⁺ cells were detected in the PDL. Two days after OTM initiation, the number of Gli1/Tomato⁺ cells increased in the PDL on the tension side. On this side, 49.3 ± 7.0% of β-catenin⁺ and 48.7 ± 5.7% of Smad4⁺ cells were found in the PDL, and Runx2 expression was detected in some Gli1/Tomato⁺ cells apart from the alveolar bone. The number of positive cells in the PDL reached a maximum on day 5. In contrast, on the compression side, β-catenin and Smad4 exhibited less immunoreactivity. On day 10, Gli1/Tomato⁺ cells were aligned on the alveolar bone on the tension side, with some expressing Runx2.</p></div><div><h3>Conclusions</h3><p>Gli1⁺ cells in the PDL differentiated into osteoblasts during OTM. Wnt and bone morphogenetic proteins signaling pathways may be involved in this differentiation.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 2","pages":"Pages 373-380"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140159190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of citrullinated α1-antitrypsin (A1AT) in saliva in a mouse model of rheumatoid arthritis","authors":"Wakako Sakaguchi ,&nbsp;Juri Saruta ,&nbsp;Yuko Yamamoto ,&nbsp;Tomoko Shimizu ,&nbsp;Shinya Fuchida ,&nbsp;Keiichi Tsukinoki","doi":"10.1016/j.job.2024.03.007","DOIUrl":"10.1016/j.job.2024.03.007","url":null,"abstract":"<div><h3>Objectives</h3><p>Rheumatoid arthritis (RA) is an autoimmune disease characterized by progressive joint destruction. Early diagnosis and treatment, before joint deformation or destruction occurs, are crucial. Identifying novel biomarkers for RA in saliva could potentially enable early detection of the disease, prior to its onset.</p></div><div><h3>Methods</h3><p>We conducted a comprehensive proteomic analysis of salivary proteins in a mouse model of RA. Proteins were identified using western blotting and enzyme-linked immunosorbent assay in the serum, saliva, and ankle joints of DBA/1JJmsSlc mice, a model of RA. Ankle joints and submandibular glands were stained with hematoxylin and eosin and immunostained, and the results were compared with those of control mice.</p></div><div><h3>Results</h3><p>Citrullinated alpha-1 antitrypsin (A1AT, 46 kDa) was commonly detected in the saliva, serum, and ankle joints of mice with severe RA and was confirmed through proteomic analysis. Western blotting showed a band corresponding to 46 kDa in the serum, saliva, and ankle joints. Immunostaining of the ankle joints with the A1AT antibody showed a strong positive signal in the synovium.</p></div><div><h3>Conclusions</h3><p>In DBA/1JJmsSlc mice, cyclic citrullinated peptide antibodies and A1AT may be involved in citrullination and contribute to the development and severity of RA, making them valuable treatment targets requiring further study.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 2","pages":"Pages 473-482"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140330215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}